Hello all, Does anyone know of a company/lab/collection center from where I can get a genomic DNA expression library of E. coli CONSTRUCTED IN A PLASMID VECTOR?? I prefer a insert size of 3 Kb or less but others are also welcome. Please suggest. Thanks, S.V. Dole ...
We are looking for a tobacco genomic library to screen and do not want to pay the companies for what is already available (or reinvent the wheel). Are there any kind and helpful souls who would be willing to share one with us? Thanks, Mark Guiltinan Penn State Biotechnology Institute mjg at psupen.psu.edu ...
A genomic library is a collection of bacteria which have been genetically engineered to hold the entire DNA of an organism. This...
Fully CpG methylated human genomic DNA, genomic DNA extracted from different tissue types, and other assorted DNA extracts can be used for a variety of epigenetic research purposes including Southern blotting, genomic library...
ATCC ® 37051™ Designation: YEp24 TypeStrain=False Application: YE-type (episomal) shuttle vector shuttle vector vector for constructing genomic libraries
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organisms DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis. There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also ...
Metagenomic profiling: microarray analysis of an environmetal genomic library. Vector systems allowing efficient autonomous or integrative gene cloning in Micromonospora sp. strain 40027
OPPORTUNITY TO PROPOSE ORGANISMS FOR BAC LIBRARY CONSTRUCTION Release Date: December 19, 2001 NOTICE: NOT-HG-02-004 National Human Genome Research Institute Annual Submission Dates: February 10, June 10 and October 10 Over the past several years, the bacterial artificial chromosome (BAC) has emerged as the vector system of choice for the construction of the large- insert chromosomal DNA libraries that are needed in genomic studies. Because BAC clones are relatively large and appear to faithfully represent an organisms genome, the BAC system will also be the vehicle of choice for the isolation of targeted regions of genomic DNA from additional organisms being used in specific biological studies, a variety of mouse strains, and even from individual humans. With the increasing interest in genomic approaches to biological research, the demand for new BAC libraries is expected to increase rapidly in the next several years. To meet the need to increase the number of available BAC libraries, NHGRI, ...
Background SELEX is a well established in vitro selection tool to analyze the structure of ligand-binding nucleic acid sequences called aptamers. Genomic SELEX transforms SELEX into a tool to discover novel, genomically encoded RNA or DNA sequences binding a ligand of interest, called genomic aptamers. Concerns have been raised regarding requirements imposed on RNA sequences undergoing SELEX selection. Methodology/Principal Findings To evaluate SELEX and assess the extent of these effects, we designed and performed a Neutral SELEX experiment omitting the selection step, such that the sequences are under the sole selective pressure of SELEXs amplification steps. Using high-throughput sequencing, we obtained thousands of full-length sequences from the initial genomic library and the pools after each of the 10 rounds of Neutral SELEX. We compared these to sequences obtained from a Genomic SELEX experiment deriving from the same initial library, but screening for RNAs binding with high affinity to the E.
ATCC ® 37524™ Designation: pWE16 TypeStrain=False Application: contains easily purifiable cassette(s) for construction expression vector integrating vector shuttle vector vector for constructing genomic libraries vector permitting RNA synthesis in vitro vector permitting positive selection for integration
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3) and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5-AATTCGGCACGAGG-3) followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine). ...
Molecular Biology Section I Gene library and screening I1 Genomic libraries I2 cDNA libraries I3 Screening procedures I1 Genomic libraries Representative gene ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 3c2165-YWNjZ
GECCO is offering development and construction of directed evolution libraries or small focused libraries, where for example several residues are being randomized ...
Best kit for cloning more than 8 kb fragment - posted in Molecular Biology Products: hi all. Im trying to prepare a genomic library of a bacteria.. please suggest me a good kit (vectors+ligation kit) which can clone a fragment of more than 8 kb shyam
Enterohemorrhagic E. coli (EHEC) O157:H7 can cause serious gastrointestinal and systemic disease in humans following direct or indirect exposure to the bacterium excreted in ruminant feces. The main colonization site of EHEC O157:H7 in cattle is the terminal rectum where the bacteria intimately attach to the epithelium and multiply in the intestinal mucus. This study aimed to identify genomic regions of EHEC O157:H7 that contribute to colonization and multiplication at this site. A bacterial artificial chromosome (BAC) library was generated from a derivative of the sequenced E. coli O157:H7 Sakai strain. The library contains 1152 clones averaging 150 kbp. To verify the library, clones containing a complete locus of enterocyte effacement (LEE) were identified by DNA hybridization. In line with a previous report, these did not confer a type III secretion capacity to the K-12 host strain. However, conjugation of one of the BAC clones into a strain containing a partial LEE deletion restored type III
Genomic DNA Library - posted in Molecular Biology: I constructed a genomic DNA library of a bacterium using Partial DNA digestion employing Lambda ZAP II Kit (Stratagene). I did everything alright as shown in kit. I used 2-4 kb fragments for ligation with pre-digested vector supplied in kit. I used 1 uL of ligate for packaging and then amplified the library. In Blue-white screening, all the plaques showed transperant or clear with blue rings at the edges. There was no blue colony. I though...
After all this is verified, we perform mass transformations, plating and pick. We use the following robotic platforms: G3 (Genomic Solutions), QPIX2 (Genetix) and MegaPIX (Genetix). The robots do blue/white selection and pick white clones into 384-well plates of glycerol storage solution. The plates of clones are grown overnight (~18 hours), and then we determine the exact plate and well position for all wells that did not grow (we call these missed wells).. We re-grid a subset of the clones onto 22cm x 22cm agar plates with XGAL+IPTG+Antibiotics and allow the colonies to grow 18 hours, then incubate for several days at a specific temp to allow any blue clones to be very obvious. We then count the blue clones and enter into a database. Example of Custom Mouse BAC Library QC Data There are several sheets in this file, including one that tracks if any "pins" on the robot are going bad. Your team will get a similar Excel file when the library is completed. The combined missed wells and blue ...
The new Centerville Library will cost no more than $3.1 million to build, Houston County Commissioners decided at Tuesday mornings meeting.
Compre online E6270S - NEBNextÆ Fast DNA Library Prep Set for Ion Torrent(TM) - 10 reactions por R$0,00. Faça seu pedido, pague-o online e receba o...
Frequent Hitters Library from ChemDiv, counting 9,450 compounds. These compounds were classified as potentially frequent hitters.
Открытая библиотека для школьников и студентов. Лекции, конспекты и учебные материалы по всем научным направлениям ...
Indicators of service exclusion include: no access to a local doctor or hospital, no access to dental treatment, no childcare for working parents, no aged care for frail older people, and no access to a bank or building society. Indicators of economic exclusion include: not having $500 in savings for use in an emergency, having to pawn or sell something in the past 12 months, not having spent $100 on a special treat in the past 12 months, and living in a jobless household ...
The construction and analysis of metagenomic (microbial community) libraries has provided knowledge of the genetics and biochemistry of noncultivable inhabitants of soil and marine communities (4, 9, 27). We have followed the same technological approach to begin to investigate the metabolic structure of the bowel community and to detect, by a functional screen, enzymes encoded by the genomes of the gut microbiota of mice. Phylogeny of bacterial communities can also be investigated by screening metagenomic libraries for 16S rRNA genes. We did not pursue this option because a catalogue of the murine gut microbiota derived from PCR-amplified 16S rRNA genes has been provided by Salzman et al. (30) and because Béjà et al. (4) have reported that the qualitative phylogenetic representation obtained with a BAC library was in general agreement with previous reports about the recovery of PCR-amplified rRNA genes from a marine community.. As in the case of soil, the digesta contains compounds that ...
To explore CR1 distribution in C. porosus, macroarrays were screened with CR1b-derived overgos or a combination of CR1a and CR1b overgos. Comparison of the CR1b- and CR1a/b-probed macroarrays revealed that the CR1a and CR1b subfamilies are not distinguishable in our assay, i.e., virtually no differences in hybridization pattern and intensity were observed when comparing the CR1b and CR1a/CR1b filters (data not shown). It was clear, however, that elements similar to CR1a and b are fairly abundant in C. porosus. Examination of one-quarter of the CR1a/b-probed macroarray (Figure 3A) indicates that 8.9% of clones show hybridization to the CR1a/b overgos while CR1b overgo hybridization to a filter stamped with the contents of a single 384-well plate from the BAC library suggests that 12.8% of clones (49 of 384) are positive for the CR1b overgo (Figure 4). Densitometric analysis of the macroarray reveals that there is a six-fold variation in positive clone hybridization intensity. If the lightest, but ...
Citation: Murdoch, B., Fu, A., Meng, Y., Li, C., Hansen, C., Snelling, W.M., Moore, S.S. 2004. Assignment of the SIAT4A gene to bovine chromosome 14 by linkage mapping of an associated microsatellite. Animal Genetics 35:146-147. Interpretive Summary: A new DNA marker for the Sialyltransferase 4A (SIAT4A) gene was developed and mapped. The marker was developed from the CHORI-240 bacterial artificial chromosome library. Cattle from an Angus-based commercial seedstock line, and two USDA-MARC reference families were genotyped. The USDA-MARC reference family genotypes were used to map the marker onto cattle chromosome 14. Technical Abstract: CHORI-240 bovine bacterial artificial chromosome library high density filters were probed with gene-specific overgo primers for Sialyltransferase 4A (SIAT4A). All positive clones were confirmed by polymerase chain reaction (PCR) with different gene-specific primers. Subsequently the clones were digested with Sau3 AI and subcloned into the E. coli cloning vector ...
Fosmids are similar to cosmids but are based on the bacterial F-plasmid. The cloning vector is limited, as a host (usually E. coli) can only contain one fosmid molecule. Fosmids can hold DNA inserts of up to 40 kb in size; often the source of the insert is random genomic DNA. A fosmid library is prepared by extracting the genomic DNA from the target organism and cloning it into the fosmid vector. The ligation mix is then packaged into phage particles and the DNA is transfected into the bacterial host. Bacterial clones propagate the fosmid library. The low copy number offers higher stability than vectors with relatively higher copy numbers, including cosmids. Fosmids may be useful for constructing stable libraries from complex genomes. Fosmids have high structural stability and have been found to maintain human DNA effectively even after 100 generations of bacterial growth. Fosmid clones were used to help assess the accuracy of the Public Human Genome Sequence. The fertility plasmid or F-plasmid ...
Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.1 kb. Constructed by Life Technologies. NOTE: based on EST sequencing of several thousand clones from this library, there appears to be a low level of mouse background in this library; please use care when evaluating sequences from this library. Note: this is a Xenopus Gene Collection library. ...
Trevor Charles provides expertise in the areas of bacteria genetics and functional genomics, environmental genomics and functional metagenomics. His group has successfully constructed cosmid metagenomic libraries from soil and activated sludge, and used phenotypic screening methods to isolate clones from these libraries encoding a number of different functions including PHA metabolism, P metabolism and quorum sensing, He will direct construction of metagenomic libraries and contribute to library screening activities and sequence and functional characterization of the isolated clones.. ...
Generate stranded, Illumina®-ready cDNA libraries from 250 pg-10 ng of total mammalian RNA using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian.
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The Library Quantification Kit provides a highly-sensitive, qPCR-based quantification method for measuring DNA libraries for Illumina-based NGS.
2. Transfer 50ul of culture to a 50ml flask with 50ml 2XYT + CM (12.5ug/ml), and incubate/shake for 14-16 hours at 250rpm. (14 hour incubation best ...
This section describes the functions in the various specialized libraries, including device ID (libdevid) and device information (libdevinfo) libraries, executable and linking format (ELF) library (libelf), kernel statistics (libkstat) and kernel VM (libkvm) libraries, and the mathematical library (libm). Readers of this section should be familiar with C programming language constructs.
This section describes the functions in the various specialized libraries, including device ID (libdevid) and device information (libdevinfo) libraries, executable and linking format (ELF) library (libelf), kernel statistics (libkstat) and kernel VM (libkvm) libraries, and the mathematical library (libm). Readers of this section should be familiar with C programming language constructs.
Container libraries by Cornwall (England : County). County Library.; 1 edition; First published in 1972; Subjects: Branch libraries, Traveling libraries
Do you need a faster, more reliable solution for DNA fragmentation and library construction? Our new NEBNext® Ultra™ II FS DNA Library Prep Kit with novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability. Learn more and request a sample! ...
Do you need a faster, more reliable solution for DNA fragmentation and library construction? Our new NEBNext® Ultra™ II FS DNA Library Prep Kit with novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability. Learn more and request a sample! ...
To align the developing chicken BAC contig physical maps with the existing linkage map, its necessary to identify BACs corresponding to the DNA-based markers on the latter. Chicken BAC libraries, derived from DNA of a single UCD001 inbred Red Jungle Fowl, have been generated by our collaborators. Characterization of the first BAC library based on BamHI partial digest fragments initially was done by filter hybridization with pools of labeled, PCR-amplified fragments based on marker or gene DNA sequences. Individual marker/BAC assignments were made by Southern hybridization of BAC DNA with individual marker probes and/or PCR analysis. In this manner, 31 markers from 9 linkage groups generated 71 BamHI BAC candidates (2.3 clones per locus). This approach is labor- and cost-intensive. Thus, we began using pools of overgo probes, that are complementary synthetic oligonucleotides extended in vitro to generate ~40 base pair, double-stranded DNA probes. Two BAC libraries were hybridized to pools of 36 ...
Studies of gene expression in grape berries require extraction of high quality RNA for construction of cDNA libraries and northern blot analyses. We have developed a procedure that yields high quality RNA from grape berries and have used it to extract RNA from fruit at five stages of development, from just after set until just after veraison. The extraction is based on a Tris-borate extraction buffer that has proven useful in other fruit tissue containing large amounts of polysaccharide. The method does not require cesium chloride centrifugation but includes a series of ethanol precipitations to remove polysaccharides. The quality of the RNA was demonstrated by constructing a cDNA library from a berry sample just prior to veraison. The library had 6 x 105 primary recombinants with an average insert size of 1.3 kb. We were able to obtain several full length clones for malic enzyme from the library, one of which was sequenced and is available on Genbank (#L-34836).. ...
We have cloned and characterized the Na,K-ATPase β3 subunit gene (ATP1B3), and a β3 subunit pseudogene (ATP1B3P1), from a human PAC genomic library. The β3 subunit gene is > 50 kb in size and is split
Cloning products and expression vectors assemble, replicate, and amplify recombinant DNA. Components are available together as part of molecular cloning kits or separately as plasmid vectors, competent cells, cDNA, ORF clones, and genomic libraries.
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The Genome Reference Consortium, in conjunction with the Pharmacogenomics Research Network, has also sought to identify and provide reference assembly representation for structural variation at the CYP2D6 locus. Much of this work was done by examining the end-sequence alignments of different fosmid libraries to the reference (Kidd et al.). As the reference assembly was known to represent CYP2D6, CYP2D7 and CYP2D8P each in single copy, we could ascertain potential duplication and deletion alleles of these genes by identification of discordant fosmid end-sequences (Figure 1). ...
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In the information age, the key to success is rapid access to the exact information you need, the needle in the great haystack of digital content. Thats why we created the Content Library - to help you quickly zero in on the facts and background necessary for good business decisions. AQUAs Content Library gathers the most important features and stories from the magazine and website under the most important content categories, and provides them to you in a clean, easy-to-access PDF. Simply complete the free online registration and this modern library of pool and spa related learning is yours ...
The Library Learning Centre (the headquarters of TU Delft Library) is open 365 days per year. During examination periods the Library has extended opening times and the whole building (with exception of Coffee Star) is a quiet zone during XXL-times. ...
The Akron-Summit County Public Library is pleased to offer a wide range of materials for borrowing with information related to borrowing library material.
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