Background: The silkworm, Bombyx mori, is one of the most economically important insects in many developing countries owing to its large-scale cultivation for silk production. With the development of genomic and biotechnological tools, B. mori has also become an important bioreactor for production of various recombinant proteins of biomedical interest. In 2004, two genome sequencing projects for B. mori were reported independently by Chinese and Japanese teams; however, the datasets were insufficient for building long genomic scaffolds which are essential for unambiguous annotation of the genome. Now, both the datasets have been merged and assembled through a joint collaboration between the two groups. Description: Integration of the two data sets of silkworm whole-genome-shotgun sequencing by the Japanese and Chinese groups together with newly obtained fosmid- and BAC-end sequences produced the best continuity (~3.7 Mb in N50 scaffold size) among the sequenced insect genomes and provided a high degree
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Comparative mapping resource for grasses (plant) +, A publicly accessible legume resource that will integrate genetic and molecular data from multiple legume species and enable cross-species comparisons +, The Honey Bee genome database +, Brassica database + and A browser-based tool for the visual comparison of various maps (sequence, genetic, etc.) from any number of species. The CMap package also includes tools for curating map data. + ...
A browser-based tool for the visual comparison of various maps (sequence, genetic, etc.) from any number of species. The CMap package also includes tools for curating map data. +, Comparative mapping resource for grasses (plant) +, A publicly accessible legume resource that will integrate genetic and molecular data from multiple legume species and enable cross-species comparisons +, The Honey Bee genome database + and Brassica database + ...
A new paper came out in Science this week, Worldwide Human Relationships Inferred from Genome-Wide Patterns of Variation, thats getting some media play. The second-to-last author is L. L. Cavalli-Sforza, and the general combination of means and ends on display in The History and Geography of Human Genes, is all over it. From the introduction:…. ...
LOCUS DF090445 668521 bp DNA linear CON 26-OCT-2017 DEFINITION Bombyx mori DNA, scaffold: Bm_scaf130, strain: p50T/Dazao. ACCESSION DF090445 BABH01000000 VERSION DF090445.1 DBLINK BioProject:PRJDA20217 BioSample:SAMD00036537 KEYWORDS WGS. SOURCE Bombyx mori (domestic silkworm) ORGANISM Bombyx mori Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Holometabola; Lepidoptera; Glossata; Ditrysia; Bombycoidea; Bombycidae; Bombycinae; Bombyx. REFERENCE 1 (bases 1 to 668521) AUTHORS Mita,K. and Xia,Q. CONSRTM The international silkworm genome sequencing consortium TITLE Direct Submission JOURNAL Submitted (06-SEP-2007) to the DDBJ/EMBL/GenBank databases. Contact:Kazuei Mita National Institute of Agrobiological Sciences, Insect Genome Research Unit; 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan REFERENCE 2 AUTHORS CONSRTM The International Silkworm Genome Consortium TITLE The genome of a lepidopteran model insect, the silkworm Bombyx mori JOURNAL Insect Biochem. Mol. ...
LOCUS DF090416 1310704 bp DNA linear CON 26-OCT-2017 DEFINITION Bombyx mori DNA, scaffold: Bm_scaf101, strain: p50T/Dazao. ACCESSION DF090416 BABH01000000 VERSION DF090416.1 DBLINK BioProject:PRJDA20217 BioSample:SAMD00036537 KEYWORDS WGS. SOURCE Bombyx mori (domestic silkworm) ORGANISM Bombyx mori Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Holometabola; Lepidoptera; Glossata; Ditrysia; Bombycoidea; Bombycidae; Bombycinae; Bombyx. REFERENCE 1 (bases 1 to 1310704) AUTHORS Mita,K. and Xia,Q. CONSRTM The international silkworm genome sequencing consortium TITLE Direct Submission JOURNAL Submitted (06-SEP-2007) to the DDBJ/EMBL/GenBank databases. Contact:Kazuei Mita National Institute of Agrobiological Sciences, Insect Genome Research Unit; 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan REFERENCE 2 AUTHORS CONSRTM The International Silkworm Genome Consortium TITLE The genome of a lepidopteran model insect, the silkworm Bombyx mori JOURNAL Insect Biochem. Mol. ...
The modENCODE Project will try to identify all of the sequence-based functional elements in the Caenorhabditis elegans and Drosophila melanogaster genomes." ...
Western biology professor Graham Thompson claims that he and Peter Oxley of the University of Sydney in Australia have for the first time isolated a region on the honey bee genome that houses the selfish gene controlling sterility in female workers bees… [Editors Note: The research will be published in the July issue of Genetics ...
View the Fent te surname, family crest and coat of arms. Discover the Fent te family history for the French Origin. What is the origin of the name Fent te?
Identification of components of the intracellular transport machinery of acylated proteins by a genome-wide RNAi screen [Elektronische Ressource] / presented by Julia Ritzerfeld : IDENTIFICATIO N O F CO M PO NENTS O F TH E INTRACELLU LAR TRANSPO RT M ACH INERY O F ACYLATED PRO TEINS BY A GENO M E‐W IDE RNAI SCREEN DISSERTATIO N submitted to the Combined Faculties for the Natural Sciences and for Mathematics of the Ruperto Caro la University of Heidelberg, Germany for the degree of Doctor of Natural Sciences Julia Ritzerfeld
Citation: Collins, A.M., Caperna, T.J., Williams, V.P., Garrett, W.M., Evans, J.D. 2006.Proteomic analyses of male contributions to honey bee sperm storage and mating. Insect Molecular Biology. 15(5):541-549. Interpretive Summary: The honey bee is an important agricultural insect by pollinating more than 90 crops and providing honey and other commercial products. This work reflects two longstanding goals of bee research: improving the egg-laying lifespan of queens and developing methods for preserving genetic lines that show disease resistance and other desirable traits. The latter goal is helpful for beekeepers in the short term and essential for the exploiting new data from the Honey Bee Genome Project in the long term. Here we search the bee genome for matches to proteins collected from male honey bees and from semen itself. We discuss dozens of such matches and show which ones are likely to be important in sperm survival and the effective fertilization of queens. The results will aid ...
In the December 2017 VectorBase release VectorBase announced the adoption of the new Aedes aegypti AaegL5 genome assembly produced by the Aedes aegypti Genome Working group. We have received a number of communications from the community that indicate people are encountering significant problems when working with the new gene set based on this assembly. The NCBI Aedes aegypti annotation release 101 (https://www.ncbi.nlm.nih.gov/genome/annotation_euk/Aedes_aegypti/101/) was a new de novo annotation using the Gnomon software (https://www.ncbi.nlm.nih.gov/genome/annotation_euk/gnomon/), and as such differs significantly from the existing AaegL3 gene set. VectorBase has worked with the NCBI to attempt to map existing stable gene ids between the assemblies, but given the nature of the different assembly and predicted gene models this has not always been possible. Improved contiguation of the AaegL5 assembly has resulted in significant gene model changes at a number of loci as a result of split/merge ...
The honey bee is an excellent model system for studying biological systems including social behavior and gene environment interactions. As a model system, the honey bee is relatively inexpensive when compared to a rodent model, yet still possesses some of the complex attributes such as a social structure and the ability of individuals to learn and communicate with other members of its society. These characteristics make it particularly attractive as a model for studying neurobiology. While possessing these complex attributes, at the same time, the honey bee is a simpler biological model than a higher order animal and as such, offers an increased likelihood for identifying biological molecules or collections of molecules involved in the condition of interest. This can be extremely valuable for dissecting the underlying causes for health and disease when those molecules or collections of molecules have human orthologs. In October of this year, the first draft of the honey bee genome was published ...
April Flowers for redOrbit.com - Your Universe Online. A new study suggests that the reason worker bees are such a highly skilled and specialized workforce is that the genes controlling their behavior are re-shuffled frequently, helping evolution build a better bee.. The new research from York University, published in the Proceedings of the National Academy of Sciences (PNAS), sheds light on how sterile worker bees evolved charismatic and cooperative behaviors. These behaviors include nursing young bees, collecting food for the colony, defending it against intruders, and dancing to communicate the location of profitable flowers to nest mates.. By examining the honey bee genome, the team noticed that the genes associated with worker behavior were found in the area of the genome known to have the highest rate of recombination, which is basically a shuffling of the genetic deck. Biology Professor Amro Zayed says that because of such shuffling, the bees can be strongly varied. For example the ...
FlyBase is an online database of Drosophila (fruit fly) genes and genomes. The FlyBase API allows users to retrieve Chado XML data for FlyBase objects using either the primary FlyBase ID or current symbol. Searchable objects include genes, alleles, transcripts, proteins, clones, etc. The XML data returned contains most of the data used to create FlyBases web reports, excluding images and gene summary information ...
The identifier 17018967 is not present in the current release of the VectorBase database.. This view requires a gene, transcript or protein identifier in the URL. For example: ...
Cornell researchers have played a major role in an international scientific team that has compared the complete set of genes of 12 closely related fruit fly species. As well has having implications for human health, the analysis paves the way for better understanding the evolution of each species.
Description: There is extraordinary diversity in sexual dimorphism (SD) among animals, but little is known about its epigenetic basis. To study the epigenetic architecture of SD in a haplodiploid system, we performed RNA-seq and whole-genome bisulfite sequencing of adult females and males from two closely related parasitoid wasps, Nasonia vitripennis and Nasonia giraulti. More than 75% of expressed genes displayed significantly sex-biased expression. As a consequence, expression profiles are more similar between species within each sex than between sexes within each species. Furthermore, extremely male- and female-biased genes are enriched for totally different functional categories: male-biased genes for key enzymes in sexpheromone synthesis and female-biased genes for genes involved in epigenetic regulation of gene expression. Remarkably, just 70 highly expressed, extremely male-biased genes account for 10% of all transcripts in adult males. Unlike expression profiles, DNA methylomes are ...
Drosophila Melanogaster, the common fruit fly, is a model organism which has been extensively used in entymological research. It is one of the most studied organisms in biological research, particularly in genetics and developmental biology. When its not being used for scientific research, D. melanogaster is a common pest in homes, restaurants, and anywhere else that serves food. They are not to be confused with Tephritidae flys (also known as fruit flys). https://en.wikipedia.org/wiki/Drosophila_melanogaster
FlyBase comprises a series of electronic documents and information processing software, the reference copy of which currently resides at the Internet address ,ftp://ftp.flybase.org/,. This publication may be copied for non-commercial, scientific uses by individuals or organizations (including for-profit organizations). FlyBase is freely distributed to the scientific community on the understanding that it will not be used for commercial gain by any organization. Any commercial use of this publication, or any parts thereof, is expressly prohibited without permission in writing from the FlyBase consortium. Certain portions of FlyBase are copyrighted separately. This notice does not invalidate any prior copyright pertaining to portions of FlyBase. Warranty FlyBase and the Genetics Society of America make no warranty of any kind with respect to the subject matter included herein. FlyBase and the Genetics Society of America specifically disclaim all warranties, expressed, implied or otherwise, ...
FlyBase comprises a series of electronic documents and information processing software, the reference copy of which currently resides at the Internet address ,ftp://ftp.flybase.org/,. This publication may be copied for non-commercial, scientific uses by individuals or organizations (including for-profit organizations). FlyBase is freely distributed to the scientific community on the understanding that it will not be used for commercial gain by any organization. Any commercial use of this publication, or any parts thereof, is expressly prohibited without permission in writing from the FlyBase consortium. Certain portions of FlyBase are copyrighted separately. This notice does not invalidate any prior copyright pertaining to portions of FlyBase. Warranty FlyBase and the Genetics Society of America make no warranty of any kind with respect to the subject matter included herein. FlyBase and the Genetics Society of America specifically disclaim all warranties, expressed, implied or otherwise, ...
Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved invasion suppressors.. RNAi line: 41021 (X ...
Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved invasion suppressors.. RNAi line: 25622 (III ...
abstract = {BACKGROUND: The Drosophila melanogaster genome contains 29 serpin genes, 12 as single transcripts and 17 within 6 gene clusters. Many of these serpins have a conserved "hinge" motif characteristic of active proteinase inhibitors. However, a substantial proportion (42%) lacks this motif and represents non-inhibitory serpin-fold proteins of unknown function. Currently, it is not known whether orthologous, inhibitory serpin genes retain the same target proteinase specificity within the Drosophilid lineage, nor whether they give rise to non-inhibitory serpin-fold proteins or other, more diverged, proteins. RESULTS: We collated 188 orthologues to the D. melanogaster serpins from the other 11 Drosophilid genomes and used synteny to find further family members, raising the total to 226, or 71% of the number of orthologues expected assuming complete conservation across all 12 Drosophilid species. In general the sequence constraints on the serpin-fold itself are loose. The critical Reactive ...
abstract = {BACKGROUND: The Drosophila melanogaster genome contains 29 serpin genes, 12 as single transcripts and 17 within 6 gene clusters. Many of these serpins have a conserved "hinge" motif characteristic of active proteinase inhibitors. However, a substantial proportion (42%) lacks this motif and represents non-inhibitory serpin-fold proteins of unknown function. Currently, it is not known whether orthologous, inhibitory serpin genes retain the same target proteinase specificity within the Drosophilid lineage, nor whether they give rise to non-inhibitory serpin-fold proteins or other, more diverged, proteins. RESULTS: We collated 188 orthologues to the D. melanogaster serpins from the other 11 Drosophilid genomes and used synteny to find further family members, raising the total to 226, or 71% of the number of orthologues expected assuming complete conservation across all 12 Drosophilid species. In general the sequence constraints on the serpin-fold itself are loose. The critical Reactive ...
Hi. I am trying to look at puff genes across the Drosophila melanogaster genome. Is there a downloadable file somewhere with the genome locations of the puffs, preferably with stage information? Any advice would be great. Thanks very much. Rebecca ...
In this paper, we describe the results of a knockdown screen in mouse ES cells to identify factors required for differentiation. Grouping of the identified genes into functional pathways shows that multiple hits are involved in Ras-Mek-Erk signaling. EphB4 receptors can regulate the activity of the Ras family of GTPases, including H-Ras and R-Ras (Zou et al., 1999; Miao et al., 2001; Wang et al., 2006). When Ptpn11 (also called Shp-2), another hit from our screen, was prevented from interacting with a mutated gp130 receptor that failed to activate ERKs, this led to self-renewal (Burdon et al., 1999). These data show that our unbiased, genome-wide knockdown approach identified several factors that were previously identified to be important in self-renewal of ES cells and validate our screening strategy. An shRNA against Capn10 was found in ∼50% of the sequences and, when tested individually, this shRNA showed strong ES colony outgrowth during the first 2 wk after removal of LIF. During the 3rd ...
In the present study, a genome-wide RNA interference screen was combined with an extensive biochemical analysis and quantitative proteomics to better understand the regulation of the heat-shock response (HSR) upon thermal stress. The usage of an endoribon...
drosophila definition: Any of numerous tiny fruit flies for the genus Drosophila, specially D. melanogaster, used extensively in hereditary analysis.; Any fruit fly regarding the genus Drosophila; A genus…
The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...
The "175" family is distinct from MULE families described so far in Arabidopsis (Yu et al., 2000), supporting the claim that short RNA sequences can draw attention to previously unidentified TE families. The longest copy, which contains the coding region of MudrA transposase, is possibly an autonomous element that has degenerated rapidly to produce a heterogeneous group of internally deleted, nonautonomous derivatives (Fig. 2). The short RNA could originate from either the left (F14F8, T4B21) or right (F14J22, F25O24) half of the TIR of an internally deleted copy (Fig. 2). The existence of short RNAs derived from the TIR region suggests transcription through the entire element and intramolecular pairing to form a dsRNA, which would probably be produced most readily with transcripts issuing from one of the more extensively deleted copies (Fig. 2).. The "175" short RNAs are less abundant than the "40" family of short RNAs, as indicated by the recovery of fewer independent clones and a fainter ...
The goals of the Drosophila Genome Center are to finish the sequence of the euchromatic genome of Drosophila melanogaster to high quality and to generate and maintain biological annotations of this sequence. In addition to genomic sequencing, the BDGP is 1) producing gene disruptions using P element-mediated mutagenesis on a scale unprecedented in metazoans; 2) characterizing the sequence and expression of cDNAs; and 3) developing informatics tools that support the experimental process, identify features of DNA sequence, and allow us to present up-to-date information about the annotated sequence to the research community. [Information of the supplier ...
In this study, we have used an automated image analysis pipeline to screen through images from a high-content, genome-wide RNAi screen for genes whose activity is rate-limiting for the growth of Drosophila cells in culture. In doing so, we identified a number of known and novel genes regulating cell size. Interestingly, this screen identified a novel role for autocrine signaling through Pvfs and the receptor tyrosine kinase Pvr in the control of the autonomous growth of Drosophila cells in culture. Previous studies have suggested roles for Pvf/Pvr signaling in the control of cell migration [23, 27, 30], morphogenesis [25, 26, 31], cell viability [22] and proliferation [28, 29]. However, to our knowledge this is the first clear example of this pathway controlling cell size. This reduction in the size of Pvr RNAi cells was accompanied by a reduction in cell proliferation, as revealed by reduced cell numbers in the absence of significant apoptosis (data not shown), and by a delay in the passage of ...
The mir-2 microRNA family includes the microRNA genes mir-2 and mir-13 (MIPF0000049). Mir-2 is widespread in invertebrates, and it is the largest family of microRNAs in the model species Drosophila melanogaster. MicroRNAs from this family are produced from the 3 arm of the precursor hairpin. Leaman et al. showed that the miR-2 family regulates cell survival by translational repression of proapoptotic factors. Based on computational prediction of targets, a role in neural development and maintenance has been suggested. The mir-2 family is specific to protostomes. There are 8 mir-2-related loci in Drosophila melanogaster: mir-2a-1, mir-2a-2, mir-2b-1, mir-2b-2, mir-2c, mir-13a, mir-13b-1 and mir-13b-2. Most other insect genomes host five mir-2 loci although the number varies in other invertebrates. Mir-13 subfamily emerged from mir-2 sequences before the insect radiation. Although mir-11 and mir-6 have similar sequences to mir-2 microRNAs, they are not evolutionarily related, and therefore should ...
Vector biology, population genetics. My overall research interest is in the population genetics of insect vectors of human and animal diseases. I have developed a program that pursues knowledge that may be applied to the control of vectorborne diseases but at the same time addresses critical issues in basic evolutionary genetics. My work has transitioned from classical population genetics to a more contemporary population genomics approach. Whereas the earlier work was based on analyses using genic markers, such as microsatellite DNA and single nucleotide polymorphisms, our current work applies next generation sequencing to study individual insect genomes, allowing us to explore problems with far greater depth and to address questions that were intractable just a few years ago. In parallel with our increasing use of genomics I have established a program in bioinformatics which is essential for both the management and analysis of the large body of data we are generating using next generation ...
WHAT a wonderful time to be a biologist! This month we celebrate the publication of the "complete" genomic sequences of 10 species of Drosophila to complement those of Drosophila melanogaster (Adams et al. 2000) and D. pseudoobscura (Richards et al. 2005). A 13th genome, that of D. mauritiana, has been sequenced in part, but the data have yet to be analyzed (L. Hillier, Washington University, personal communication). This achievement is marked by the publication of two "community" articles in Nature in November 2007 (Drosophila 12 Genomes Consortium 2007; Stark et al. 2007) and nearly 50 articles in other journals, including Genetics. Rather than attempt to summarize these articles (in effect this is done by the community publications), here I will attempt to draw some more general biological and sociological lessons learned from the sequencing of these genomes.. What are the deep biological questions that large-scale sequencing should seek to answer, in particular large-scale sequencing of ...
Background Apoptosis, one of many types of programmed cell loss of life, is conducted and regulated with a organic proteins network. vertebrate, nematode, and insect genomes, got multiple paralogs in the cnidarian-bilaterian ancestor. Different people of the ancestral Apaf-1 family members resulted in the extant protein in nematodes/pests and in deuterostomes, detailing significant functional differences between proteins that until had been thought to be orthologous today. Similarly, 173220-07-0 IC50 the advancement from the Bcl-2 and caspase proteins families appears 173220-07-0 IC50 amazingly complicated and evidently included significant gene reduction in nematodes and pests and expansions in deuterostomes. Bottom line The rising picture from the evolution from the apoptosis network is certainly among a succession of lineage-specific expansions and loss, which combined with limited amount of apoptotic proteins families, led to apparent commonalities between systems in different ...
Read more about Genomic boost to protect Assams muga silkworm on Business Standard. A snapshot of the muga silkworm genome decoded by Indian scientists offers vital clues for imparting disease-resistance to help conserve the economically important insect which spins the distinctive golden yellow fabric synonymous with Assam.
...WEST LAFAYETTE Ind. - Wheats genetic resistance to Hessian flies has...The Purdue and USDA research team developed a method to test toxins fr... For years people have tried to develop a bioassay but that hadnt h...Shukle said the 33 genes known to give wheat resistance to Hessian fly...,Wheat,cant,stop,Hessian,flies,,so,scientists,find,reinforcements,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
Locale, Genomes and Genes, Scientific Experts, Publications, Species, Research Topics about Experts and Doctors on drosophila melanogaster in New York, United States
A genome-wide RNAi screen in Drosophila S2 cells identified 41 genes capable of specifically modifying Aβ secretion without affecting general secretion or viability. As expected, the four γ-secretase complex components showed the most potent Aβ-lowering effects upon knockdown, with ,10% Aβ remaining. This validated the RNAi screen because it demonstrated that the approach could robustly identify relevant Aβ-modulator genes in an unbiased screen. There was a large difference in the magnitude of phenotype between these four genes and the other genes identified, suggesting that no other essential γ-secretase complex protein exists as expected (Edbauer et al., 2003), but rather proteins that serve a modulatory role in Aβ generation. Indeed, another high-throughput RNAi screen found a potent modulator of BACE1 activity but did not describe the identification of any γ-secretase modulators (Majercak et al., 2006). Knockdown of pigeon, the Drosophila homolog of the recently identified GSAP ...
Evolutionarily conserved functional domains of non-coding RNAon chromosome X (roX1 ) have been identified in eight Drosophilaspecies in a prior study.
Francioli, L. C.; Polak, P. P.; Koren, A.; Menelaou, A.; Chun, S.; Renkens, I.; Genome of the Netherlands, C.; van Duijn, C. M.; Swertz, M.; Wijmenga, C. et al.; van Ommen, G.; Slagboom, P. E.; Boomsma, D. I.; Ye, K.; Guryev, V.; Arndt, P. F.; Kloosterman, W. P.; de Bakker, P. I.; Sunyaev, S. R.: Genome-wide patterns and properties of de novo mutations in humans. Nature Genetics 47 (7), pp. 822 - 826 (2015 ...
FlyBase update October 20, 1993 - new P1 clone and P element lists G. M. Rubin and the Drosophila Genome Center have made updated lists of P1 clones and new lists of P element lines available to FlyBase. The lists can be accessed either through the FlyBase Gopher server or by anonymous ftp from the Flybase server (fly.bio.indiana.edu). There are now 2038 P1 clones available from designated distributing laboratories and 508 P element insertion lines available from the Bloomington Drosophila Stock Center. The lists are available in comma-separated value (csv) file form, which can be used by many spreadsheet and database programs, as well as in formatted tables files sorted in order of polytene chromosome band location and files used to support gopher searches by polytene location or clone or P element name. There are three documentation files. P1.doc describes the P1 clone list files, and Pelement.doc describes the P element files. LBL.doc provides information on how the P1 clones and P element ...
MicroRNAs (miRNAs) are short regulatory RNAs that inhibit target genes by complementary binding in 3 untranslated regions (3 UTRs). They are one of the most abundant classes of regulators, targeting a large fraction of all genes, making their comprehensive study a requirement for understanding regulation and development. Here we use 12 Drosophila genomes to define structural and evolutionary signatures of miRNA hairpins, which we use for their de novo discovery. We predict ,41 novel miRNA genes, which encompass many unique families, and 28 of which are validated experimentally. We also define signals for the precise start position of mature miRNAs, which suggest corrections of previously known miRNAs, often leading to drastic changes in their predicted target spectrum. We show that miRNA discovery power scales with the number and divergence of species compared, suggesting that such approaches can be successful in human as dozens of mammalian genomes become available. Interestingly, for some ...
Bordensteins team studied Nasonia parasitic wasps, which are about the size of a sesame seed, and they serve as one of the best models to dissect and characterize the evolution of insect genomes.
The analyses presented here indicate that ubc-25 acts as a negative regulator of steady-state CYE-1 expression, but it is not known whether this regulation is achieved through direct ubiquitinylation of CYE-1 by UBC-25. It is likely that UBC-25 ubiquitinylates a range of targets to regulate a variety of processes. In fact, ubc-25 was previously recognized for roles not directly related to the cell cycle, such as promoting a Ras-mediated cell-fate decision (Rocheleau et al. 2008) and maintaining neuromuscular homeostasis (Schulze et al. 2003). It would be interesting to determine whether the putative interaction partner, C30H7.2, acts with UBC-25 in these alternative processes. Our genetic analyses provide insights into the regulatory mechanisms that may explain the relatively mild loss of function phenotypes observed when individual components are inactivated. First, other genes within a family may provide redundant activity. For example, 22 ubc genes are encoded in the C. elegans genome and we ...
Supplementary MaterialsData_Sheet_1. and KDM5C attenuation affected DNA harm response and improved DNA double-strand breaks (DSBs), and reduced advancement of UV-irradiated embryos. Results from this research exposed that both KDM5B and KDM5C are essential regulators of early advancement in porcine embryos as their attenuation modified H3K4 and H3K9 methylation patterns, perturbed embryo genome activation, and reduced DNA damage restoration capability. Maturation (IVM) Ovaries of prepubertal gilts had been collected at an area slaughterhouse (Olymel S.E.C./L.P., Saint-Esprit, QC, Canada) and transferred to the lab at 32C in saline option including penicillin (100 UI/ml) and streptomycin (10 mg/ml). Cumulus-oocyte complexes (COCs) had been aspirated from 3 to 6 mm follicles utilizing a 10 mL syringe and 20-measure needle in TMP 269 distributor support of COCs having at the least three cumulus cells levels and a homogeneous granulated cytoplasm had been chosen for IVM. Sets of 30 COCs had been ...
Lidane received her B.S. in Molecular Biology from the University of California, San Diego where she was first introduced to the National Drosophila Species Stock Center. Her current role in the centers new location includes overseeing the day-to-day operations such as maintenance and additions to the collection, keeping all social media accounts up to date, hiring and managing student workers, and ensuring all species in the center are available to research labs at any time. ...