Increased soy consumption in Asian diets, resulting in increased serum isoflavone levels, has been associated with a decreased risk for prostate adenocarcinoma (PCa). The isoflavone genistein is believed to be the anticancer agent found in soy, and significant levels of genistein have been detected in human prostatic fluid, implicating the role of genistein in PCa prevention. Recent studies have demonstrated genisteins ability to inhibit cell growth and induce apoptosis in several cell lines; however, the molecular mechanisms of genisteins effect are not known. We have evaluated the mechanism by which genistein may inhibit PCa cell growth. Here we report that genistein inhibits PCa cell growth in culture in a dose-dependent manner, which is accompanied by a G2/M cell cycle arrest. Cell growth inhibition was observed with concomitant downregulation of cyclin B, upregulation of the p21WAF1 growth-inhibitory protein, and induction of apoptosis. Collectively, these results provide experimental ...

Genistein is a phytoestrogen commonly found in plants such as soybeans, lentils, and chickpeas. Humans and animals are most primarily exposed to genistein through the consumption soy and soy-based dietary products. Human exposure to genistein varies based on diet, but is present in many populations of adults and children. Genistein can bind to and signal through estrogen receptors. Therefore, it has the potential to mimic, enhance, or impair the estradiol biosynthesis pathway, and is categorized as an endocrine disrupting chemical. This is of concern to human health because genistein-induced effects on the steroidogenic pathway can result in adverse, long-term effects on female fertility, cardiovascular, bone, and overall health. The ovary is an estrogen receptor-rich tissue, and plays a key role in the regulation of female fertility. This is because the ovarys main functional unit, the follicle, is responsible for the production of sex steroid hormones and consists of the oocyte necessary for ...

The estrogenic soy isoflavone, genistein, stimulates growth of estrogen-dependent human breast cancer (MCF-7) cells in vivo. Genistin is the glycoside form of genistein and the predominant form found in plants. It is generally believed that genistin is metabolized to the aglycone genistein in the lower gut. However, it is unclear if the rate of metabolism of genistin to genistein is sufficient to produce a level of genistein capable of stimulating estrogen-dependent breast cancer cell growth. Our hypothesis was that dietary genistin would stimulate tumor growth similar to that observed with genistein in athymic mice. To test this hypothesis, genistin or genistein was fed to athymic mice containing xenografted estrogen-dependent breast tumors (MCF-7). Mice were fed either genistein at 750 p.p.m. (parts per milllion) or genistin at 1200 p.p.m., which provides equal molar concentrations of aglycone equivalents in both diets. Tumor size was measured weekly for 11 weeks. At completion of the study, ...

TY - JOUR. T1 - Genistein induces topoisomerase IIbeta- and proteasome-mediated DNA sequence rearrangements. T2 - Implications in infant leukemia. AU - Azarova, Anna M.. AU - Lin, Ren K.. AU - Tsai, Yuan Chin. AU - Liu, Leroy F.. AU - Lin, Chao P.. AU - Lyu, Yi Lisa. PY - 2010/8. Y1 - 2010/8. N2 - Genistein is a bioflavonoid enriched in soy products. However, high levels of maternal soy consumption have been linked to the development of infant leukemia ALL and AML. The majority of infant leukemia is linked to mixed lineage leukemia gene (MLL) translocations. Previous studies have implicated topoisomerase II (Top2) in genistein-induced infant leukemia. In order to understand the roles of the two Top2 isozymes in and the molecular mechanism for genistein-induced infant leukemia, we carried out studies in vitro using purified recombinant human Top2 isozymes, as well as studies in cultured mouse myeloid progenitor cells (32Dc13) and Top2β knockout mouse embryonic fibroblasts (MEFs). First, we ...

Genistein, an isoflavone, is a specific inhibitor of tyrosine kinase and topoisomerase II. However, its effect on cell growth is unknown. Therefore, we examined the effects of genistein on cell growth and cell cycle progression and compared its effects with other flavonoids. Genistein inhibited in a dose-dependent manner the growth of HGC-27 cells derived from human gastric cancer. Flow-cytometric analysis showed that genistein almost completely arrested the cell cycle progression at G2-M. This effect was reversible when genistein was removed from the culture medium. In contrast, other flavonoids such as flavone, luteolin, and the structurally similar daidzein arrested the cell cycle at G1. Consistent with the flow-cytometric analysis, microscopic observation showed that genistein did not increase the mitotic index, which supposes that genistein may arrest the cell cycle at G2 or early M. These results suggest that the G2-M arrest by genistein is a unique effect among flavonoids.. ...

It has been reported that Breast Cancer Susceptibility gene-1 & 2 (BRCA1 & 2 are potential molecular targets for chemoprevention by isoflavone genistein (4′ 5, 7-trihydroxy isoflavone), in breast and prostate cancer cells. It is also known that BRCA1 has inhibitory activity on estrogen receptor-α and genisteins action on cells is mainly through modulation of estrogen receptor activity. The action of genistein with respect to BRCA1 status in ovarian cancer cells has not been reported so far. Therefore in this study, we analyzed the action of genistein on BRCA1 antisense blocked (AS4) and unblocked (NEO) BG-1 ovarian cancer cells. We found that genistein induced comparable cytotoxic effect in both AS4 and NEO cells, but through different pathways. We found that genistein induces caspase 8 dependent apoptotic pathway in NEO cells. Genistein inhibits estrogen receptor-α and activates BARD1 in BRCA1 blocked cells and induces estrogen receptor-β and FAS in presence of BRCA1. It can be concluded ...

In the study to evaluate the effects of genistein and moderate intensity exercise on Achilles tendon collagen and cross-linking in intact and ovariectomized (OVX) female Sprague-Dawley rats, posted in PubMed, indicated that after 6 weeks, tendons were assayed for the collagen-specific amino acid hydroxyproline and hydroxylyslpyridinoline (HP). Collagen content was not influenced by exercise (P = 0.40) but was lower (P , 0.001) in OVX-vehicle rats compared with intact vehicle rats (OVX: 894 ± 35 μg collagen/mg dry weight; intact: 1185 ± 72 μg collagen/mg dry weight). In contrast, collagen content in OVX rats treated with genistein was greater (P = 0.010, 1198 ± 121 μg collagen/mg dry weight) when compared with untreated rats and was not different from intact rats (P = 0.89). HP content was lower in OVX genistein-treated rats when compared with intact genistein-treated rats, but only within the sedentary animals (P = 0.05, intact-treated: 232 ± 39 mmol/mol collagen; OVX-treated: 144 ± 21 ...

The soy isoflavone genistein targets adipose tissue and elicits physiological effects that may vary based on dietary intake. We hypothesized that the adipose effects of genistein are dose and gender dependent. Four-week-old C57BL/6 male and female mice received daily oral doses of genistein (50-200, …

In todays study we investigated the consequences of genistein on adipogenic differentiation of mouse bone tissue marrow-derived mesenchymal stem cell (BMSC) cultures and its own potential signaling pathway. differentiation. Genistein decreased the phosphorylation of ERK1/2 in mouse BMSC ethnicities dose-dependently. Genistein incubation for the whole tradition period in adition to that applied through the early stage of the tradition period considerably inhibited Rabbit Polyclonal to Cox1. Vorinostat the adipogenic Vorinostat differentiation of mouse BMSC ethnicities. While genistein was incubated in the past due stage (after day time 9) no inhibitory influence on adipogenic differentiation was noticed. BMSC ethnicities treated with genistein in the current presence of fibroblast growth element-2 (FGF-2) an activator from the ERK1/2 signaling pathway indicated normal degrees of ERK1/2 activity and by doing this can handle going through adipogenesis. Our outcomes claim Vorinostat that activation ...

Tytuł projektu: Udostępnianie cyfrowe zasobów polskich czasopism z nauk przyrodniczych i rolniczych w bazie AGRO. Nr umowy: POPC.02.03.01-00-0038/18-00 (okres realizacji 2018-2021). Kwota dofinansowania: 7 442 980,00 z. W ramach Programu Operacyjnego Polska Cyfrowa na lata 2014-2020, Oś Priorytetowa nr 2 E-administracja i otwarty rząd Działanie nr 2.3 Cyfrowa dostępność i użyteczność informacji sektora publicznego Poddziałanie nr 2.3.1 Cyfrowe udostępnienie informacji sektora publicznego ze źródeł administracyjnych i zasobów nauki (typ projektu: cyfrowe udostępnienie zasobów nauki) Instytucja Finansująca: Centrum Projektów Polska Cyfrowa ...

Background: Morphine is a member of the naturally occurring phenanthrene alkaloids of opium. Genistein is a phytoestrogen, present in soy products. This study was designed to evaluate protective effects of genistein against morphine induced damages to the kidneys of mice. Methods: In this study, 48 male mice were randomly assigned to 8 groups: control (saline), morphine treated group (10 mg/kg/day); genistein groups (1, 2, 4 mg/kg/day) and morphine plus genistein treated group. Drugs were administrated intraperitoneally for 30 consequent days. Weight of animals and kidneys, glomeruli characteristics, kidney function markers and blood serum nitric oxide level has been studied. Result: The results indicated that morphine administration significantly increased Lactate dehydrogenase (LDH), Blood urea nitrogen (BUN), creatinine and nitric oxide levels compared to the control ( saline) group (P|0.05). Genistein in all doses and genistein plus morphine at the dose of 4 mg/kg significantly decreased LDH, BUN,

Genistein can reduce insulin resistance, oxidative and inflammatory indices along with improvement in fat metabolism in patients with NAFLD.

Genistein can be a soy derived isoflavone with many bio chemical effects, including the alteration of cell cycle reg ulatory kinase activities, Prior studies indicated that genistein induced apoptosis, enhanced the induction of apoptosis by chemotherapeutic agents, and elevated radiosensitivity in quite a few cancer cell lines, Genistein can be known as an estrogen receptor agonist, and that genistein can antagonise the proliferation of breast cancer cells by estradiol, On the other hand, most transcription activation bioassays are usually not ready to show an estrogen receptor antagonism of genistein, and genistein acts addi tive to estradiol in theses systems, Its therefore con troversial no matter if anti proliferative result of genistein is ER dependent or not, Indole three carbinol, derived from Cruciferous vegeta bles, continues to be shown to suppress the growth of a variety of tumor cells including colon cancer cells by arresting the cell cycle at G1 S and inducing apoptosis in vitro, ...

Abstract: : Purpose: To determine the effect of orally administered genistein on the blue light damage of retina within the senescence-accelerated mouse(SAM)at 28 weeks of exposure Methods: The blue light exposure was set up according to Gottsch et al (Arch Ophthalmol 1993;111:126). Forty-eight SAM P8 mice were divided into four groups. Within each group, the mice were exposed to continuous blue light. One group received a normal, genistein-free diet, one a fatty diet, one a fat+genistein diet, and one a genistein-rich diet. The mice were treated in this fashion for 28 weeks, beginning 4 weeks after birth. The effect of genistein was evaluated using electron microscopy at this specific time. Results: After 28 weeks, using electron microscopy, P8 mice receiving constant blue light and a fatty diet showed a significant thickening of Bruchs membrane and an increase in RPE-deposits compared with the mice receiving the other diet groups. Those with the pure fat diet showed atrophy of the ...

TY - JOUR. T1 - Genistein, an epigenome modifier during cancer prevention. AU - Zhang, Yukun. AU - Chen, Hong. PY - 2011/7. Y1 - 2011/7. N2 - Dietary compounds have been observed to have a great potential to regulate the epigenome, which is disrupted and reprogrammed during carcinogenesis. Because of their close association with cancer development, DNA methylation patterns have been used as a crucial marker for the study of cancer-related epigenetics. There is immense evidence indicating that dietary components play a critical role in cancer development. Genistein, one of the soy-derived bioactive isoflavones, affects tumorigenesis through epigenetic regulations. By modulating chromatin configuration and DNA methylation, genistein activates tumor suppressor genes and affects cancer cell survival. Here, we summarize and discuss both in vitro and in vivo studies in the field that investigate the effect of genistein on histone modifications and DNA methylation. The promising role of genistein in ...

TY - JOUR. T1 - Association of dietary soy genistein intake with lung function and asthma control. T2 - A post-hoc analysis of patients enrolled in a prospective multicentre clinical trial. AU - Bime, Christian. AU - Wei, Christine Y.. AU - Holbrook, Janet. AU - Smith, Lewis J.. AU - Wise, Robert A.. PY - 2012/12. Y1 - 2012/12. N2 - Background: Broad dietary patterns have been linked to asthma but the relative contribution of specific nutrients is unclear. Soy genistein has important anti-inflammatory and other biological effects that might be beneficial in asthma. A positive association was previously reported between soy genistein intake and lung function but not with asthma exacerbations. Aims: To conduct a post-hoc analysis of patients with inadequately controlled asthma enrolled in a prospective multicentre clinical trial to replicate this association. Methods: A total of 300 study participants were included in the analysis. Dietary soy genistein intake was measured using the Block Soy ...

TY - JOUR. T1 - Genistein Inhibits Prostate Cancer Cell Growth by Targeting miR-34a and Oncogenic HOTAIR. AU - Chiyomaru, Takeshi. AU - Yamamura, Soichiro. AU - Fukuhara, Shinichiro. AU - Yoshino, Hirofumi. AU - Kinoshita, Takashi. AU - Majid, Shahana. AU - Saini, Sharanjot. AU - Chang, Inik. AU - Tanaka, Yuichiro. AU - Enokida, Hideki. AU - Seki, Naohiko. AU - Nakagawa, Masayuki. AU - Dahiya, Rajvir. PY - 2013/8/1. Y1 - 2013/8/1. N2 - Objective:Genistein is a soy isoflavone that has antitumor activity both in vitro and in vivo. It has been shown that genistein inhibits many type of cancers including prostate cancer (PCa) by regulating several cell signaling pathways and microRNAs (miRNAs). Recent studies suggest that the long non-coding RNAs (lncRNAs) are also involved in many cellular processes. At present there are no reports about the relationship between gensitein, miRNAs and lncRNAs. In this study, we focused on miRNAs, lncRNA that are regulated by genistein and investigated their ...

Title: Phytochemical Genistein in the Regulation of Vascular Function: New Insights. VOLUME: 14 ISSUE: 24. Author(s):Hongwei Si, Dongmin Liu, Hongwei Si and Dongmin Liu. Affiliation:Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA., Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.. Keywords:Genistein, endothelial cells, inflammation, cAMP, nitric oxide, peroxisome proliferator-activated receptor, antioxidant, atherosclerosis. Abstract: Genistein, a natural bioactive compound derived from legumes, has drawn wide attention during the last decade because of its potentially beneficial effects on some human degenerative diseases. It has a weak estrogenic effect and is a well-known non-specific tyrosine kinase inhibitor at pharmacological doses. Epidemiological studies show that genistein intake is inversely associated with the risk of ...

Genistin is an isoflavone found in a number of dietary plants like soy and kudzu. It was first isolated in 1931 from the 90% methanol extract of a soybean meal, when it was found that hydrolysis with hydrochloric acid produced 1 mole each of genistein and glucose. Chemically it is the 7-O-beta-D-glucoside form of genistein and is the predominant form of the isoflavone naturally occurring in plants. In fact, studies in the 1970s revealed that 99% of the isoflavonoid compounds in soy are present as their glucosides. The glucosides are converted by digestive enzymes in the digestive system to exert their biological effects. Genistin is also converted to a more familiar genistein, thus, the biological activities including antiatherosclerotic, estrogenic and anticancer effects are analogous. When ingested along the diet, genistin is readily converted to its aglycone form, genistein. It is hydrolyzed by removing the covalently bound glucose to form genistein and that genistein is the form of the ...

Tyrosine kinase (TK) inhibitors genistein and tyrphostin A23 (A23) inhibited Ca2+ currents in guinea-pig ventricular myocytes investigated under regular whole-cell circumstances (K+-free of charge Tyrodes superfusate; EGTA-buffered (pCaC10. (e.g., Body 5d). Raf265 derivative In six tests, the existing induced by 100 ?0.510.07 pA pF?1 in the lack of Ni2+ (Body 5b)). Open up in another window Body 5 Ramifications of A23, genistein, and Ni2+ on membrane currents in myocytes superfused with Ca2+-free of charge alternative and dialysed with pCa 7 alternative. The myocytes had been pulsed from ?40 mV to various other voltages HDAC10 for 200 ms at 0.2 Hz before, 5 min after application of 100 romantic relationship of A23-induced current under near-physiological ionic circumstances, myocytes had been dialysed using a 7 mM Na+ pipette solution and superfused with K+-free of charge Tyrodes solution that was supplemented with 10 A23 focus is proven in Body 7, as well as the Hill equation fitting the info ...

Canine cell lines. The nonadherent canine B-cell lines 17-71 and GL-1 have been previously described (33, 34). Both lines were maintained in RPMI 1640 (Mediatech, Inc.) plus 10% heat-inactivated fetal bovine serum (Mediatch, Inc.), 1% L-glutamine (Mediatech, Inc.), and 100 mg/mL primocin (InvivoGen), and grown at 37°C/5% CO2 in a humidified atmosphere.. Genistein treatment. Genistein (Sigma) was dissolved in DMSO to make a 200 mg/mL stock solution that was added directly to the culture medium to produce various concentrations. The same volume of DMSO was added to untreated control cells, which produced a final concentration of DMSO in the range of 0.001 to 0.045%. GCP is a functional supplement developed and donated by Amino Up Chemical Co. Ltd. The detailed information of this product is provided on the website.3 Compositional analysis of the GCP extract supplied by the manufacturer revealed that 116 ± 8.4 mg of genistein, 28.5 ± 5.4 mg of daidzein, 13.5 ± 2.6 mg of glycitein, and ∼3% of ...

Steady state and time resolved fluorescence spectroscopy, combined with molecular dynamics simulation, have been used to explore the interactions of a therapeutically important bioflavonoid, genistein, with normal human hemoglobin (HbA). Binding constants estimated from the fluorescence studies were K = (3.5 ± 0.32) ×104M-1 for genistein. Specific interactions with HbA were confirmed from flavonoid-induced fluorescence quenching of the tryptophan in the protein HbA. The mechanism of this quenching involves both static and dynamic components as indicated by: (a) increase in the values of Stern-Volmer quenching constants with temperatures, (b) / is slightly > 1 (where and are the unquenched and quenched tryptophan fluorescence lifetimes (averaged) respectively). Molecular docking and dynamic simulations reveal that genistein binds between the subunits of HbA, ~18 ? away from the closest heme group of chain α1, emphasizing the fact that the drug does not interfere with oxygen binding site of HbA.

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Genistein is a natural product found in soy beans; its consumption has been associated with a low incidence of metastatic prostate cancer. Genistein is

The researchers also discovered that the naturally occurring phytoestrogen in soybean known as genistein, produced an additive estrogenic effect when combined with glyphosate, raising the serious question as to whether GMO soybeans are contributing to the epidemic levels of breast cancer within countries like the US where they are consumed in relatively high quantities.. It should be noted that the concentrations used to determine the interactive effects of glyphosate and phytoestrogen genistein in this study were modeled as in a real world situation by using information obtained from studies that assayed the respective levels of genistein and glyphosate in GM soybeans, as well as human plasma and urine concentrations following their consumption and/or exposure. For instance, glyphosate concentrations have been detected within human urine within the 0.1 - 233 parts per billion range on the lowest end, and an estimated systemic dose of 0.004 mg/kg on the high end.. ...

COPD is associated with chronic inflammation predominantly affecting the lung parenchyma and peripheral airways, resulting in largely irreversible and progressive airflow limitation. This inflammation is characterized by increased levels of alveolar macrophages, neutrophils and T lymphocytes, which are recruited from the circulation. Oxidative stress is important in driving this inflammation (1). NF-κB is known to be critical in the regulation of proinflammatory molecules during cellular responses, particularly TNF-α, IL-6 and IL-8. When phosphorylated, IκB dissociates from the NF-κB-IκB complex, resulting in the translocation of NF-κB from the cytoplasm to the nucleus. Activation of NF-κB is regarded as an important initial event in the airway inflammatory response to a variety of stimuli, including infectious agents, toxins, cytokines, growth factors and oxidant stress (1,5,18). NF-κB may represent a link between inflammation and oxidative stress in chronic inflammatory diseases (19). ...

IRs have been extensively studied as mediators of insulins action in the periphery but also are found in the CNS (Raizada et al., 1988). With regard to the present study, insulin is known to alter stimulus-induced NE release and clearance of NE from extracellular space (Bhagat et al., 1981; Christensen, 1983; Shimosawa et al., 1992;Townsend et al., 1992). SK-N-SH cells possess IRs with a subunit composition similar to that reported for whole brain and neuronal tissue (Ota et al., 1988a; Raizada et al., 1988). In SK-N-SH cells, insulin binds to its receptors with aKd of ∼1 nM and stimulates 2-deoxyglucose uptake with an EC50 of 1 nM (Ota et al., 1988a).. IR is a membrane-bound tyrosine kinase whose activation leads to tyrosine autophosphorylation and the phosphorylation of IR-associated substrates. Consistent with this model, the tyrosine kinase inhibitor genistein, but not the inactive analog genistin, blocked the ability of insulin to enhance NE transport activity. One of the downstream ...

NEW YORK (Reuters Health) - Despite some concerns to the contrary, the soy-based dietary supplement genistein may not harm postmenopausal womens thyroid...

y assessed he said. Pregnant women are exposed to hundreds of compoun... Our study demonstrates there are highly sensitive windows early in de...His earlier research demonstrated that four common nutritional supplem... There could be additive or synergistic effects between folic acid and...Of related concern soy is a staple of almost all laboratory mouse die...,Prenatal,genistein,in,soy,reduces,obesity,in,offspring,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters

Mice eating a diet laced with an estrogen-like constituent of soy display a puzzling variety of changes, some apparently good, some potentially bad.

We can supply natural pure herbal sophora japonica fruit extract genistein powder,genisteol as leading manufacturers and suppliers,with professional factory we can offer you pure high quality and best price raw material soybean soy isoflavone genistein daidzein for supplements with healthy benefits.

BACKGROUND AND PURPOSE: Glucocorticoid-induced osteoporosis (GIO) is the leading cause of secondary osteoporosis. Clinical evidence suggests a role for genistein aglycone in the treatment of post-menopausal osteopenia although proof of efficacy in comparison with currently available treatments is still lacking. To clarify this issue, we investigated the effects of genistein on bone compared with alendronate in experimental GIO. EXPERIMENTAL APPROACH: A total of 28 female Sprague-Dawley rats were used. GIO was induced by daily injections of methylprednisolone (MP; 30 mg x kg(-1) s.c.) for 60 days. Sham GIO animals (Sham-MP) were injected daily with the MP vehicle. At the end of the osteoporosis development period, MP rats were randomized to receive: vehicle (n= 7), genistein aglycone (5 mg x kg(-1) s.c.; n= 7) or alendronate (0.03 mg x kg(-1) s.c.; n= 7). Treatment lasted 60 days. Sham-MP animals were treated with vehicle for an additional 60 days. At the beginning and at the end of treatments, ...

G. Zhang, L. Qin, H. Sheng, K.W. Yeung, H.Y. Yeung, W.H. Cheung, J. Griffith, C.W. Chan, K.M. Lee, K.S. Leung, Epimedium-derived phytoestrogen exert beneficial effect on preventing steroid-associated osteonecrosis in rabbits with inhibition of both thrombosis and lipid-deposition, Bone, 2007, 40, 3, ...

TY - JOUR. T1 - Genistein alters methylation patterns in mice. AU - Kevin Day, J.. AU - Bauer, Andrew M.. AU - Desbordes, Charles. AU - Zhuang, Yi. AU - Kim, Byung Eun. AU - Newton, Leslie G.. AU - Nehra, Vedika. AU - Forsee, Kara M.. AU - MacDonald, Ruth S.. AU - Besch-Williford, Cynthia. AU - Huang, Hui-ming. AU - Lubahn, Dennis B.. PY - 2002. Y1 - 2002. N2 - In this study we examine the effect of the phytoestrogen genistein on DNA methylation. DNA methylation is thought to inhibit transcription of genes by regulating alterations in chromatin structure. Estrogenic compounds have been reported to regulate DNA methylation in a small number of studies. Additionally, phytoestrogens are believed to affect progression of some human diseases, such as estrogen-dependent cancers, osteoporosis and cardiovascular disease. Specifically, our working hypothesis is that certain soy phytoestrogens, such as genistein, may be involved in preventing the development of certain prostate and mammary cancers by ...

2569 Soy isoflavones are being investigated as potential cancer preventive agents. Two independent studies were conducted to investigate tumor modulating abilities of a mixture of isoflavones, or an isoflavone genistein on colon cancer incidence. In the first study, the incidence of colon precancer and cancer were determined in the male offspring (F1 generation) from male and female Sprague Dawley rats exposed to soy-based diets containing variable levels of soy isoflavones during their lifetime, including during pregnancy and lactation. At weaning, the F1 rats were acclimatized on their respective parental soy-based, semi-purified basal diets containing 0, 50, or 1250 mg/kg diet of soy isoflavones. At the age of 45 days, rats received one subcutaneous dose, once a week for two weeks, of saline or 15 mg/kg body weight azoxymethane (AOM), a colon-specific carcinogen. Rats were maintained on their respective experimental diets throughout the study, which terminated 26 weeks after the first dose of ...

RATIONALE: Chemoprevention therapy is the use of certain drugs to try to prevent the development or recurrence of cancer. The use of genistein may be effective in preventing breast or endometrial cancer.. PURPOSE: This randomized phase I trial is studying the effectiveness of genistein in preventing breast or endometrial cancer in healthy postmenopausal women. ...

According to the study of Genistein, an epigenome modifier during cancer prevention by Zhang Y, Chen H., posted in US National Library of Medicine National Institutes of Health, researchers found that Genistein, one of the soy-derived bioactive isoflavones, affects tumorigenesis through epigenetic regulations. By modulating chromatin configuration and DNA methylation, genistein activates tumor suppressor genes and affects cancer cell survival. Here, we summarize and discuss both in vitro and in vivo studies in the field that investigate the effect of genistein on histone modifications and DNA methylation. The promising role of genistein in cancer prevention and therapeutic applications will be discussed from an epigenetic point of view ...

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Citation: Rahal, O., Simmen, R.C. 2009. Induction of PTEN-p53 crosstalk in mammary epithelial cells: A novel mechanism of breast cancer prevention by the dietary factor genistein [Abstract]. Cancer Research. 69(Meeting Abstract Supplement):Abstract No. 3149. Interpretive Summary: Technical Abstract: Consumption of soy foods either at an early age or for lifetime has been associated with reduced risk for developing breast cancer in humans and in animal models. However, this association continues to be controversial, and the precise mechanisms for protection remain elusive. Among the soy products, the isoflavone genistein (GEN) has been widely suggested to confer mammary tumor protection. Previously we demonstrated the increased expression of tumor suppressors PTEN and p53 in mammary epithelial cells (MECs) isolated from young adult female rats fed dietary soy protein isolate (SPI) or casein (CAS) supplemented with GEN, when compared to MECs from rats fed the control (CAS) diet. Since ...

In addition to possibly preventing the development of prostate cancer, soy consumption may also help prevent its metastasis, according to both animal and human evidence. For example, a recent study published in the Journal of the National Cancer Institute reported that the level of an enzyme that allows cells to invade tissues was markedly reduced in prostate cancer patients given the soybean isoflavone genistein.18 In a separate animal study, adding isoflavones to the diet in mice was found to inhibit prostate tumor metastasis by 96%.19 Finally, in men with prostate cancer whose treatment has been unsuccessful, preliminary evidence suggests that soy isoflavones may be of benefit. Some studies, but not all, show that isoflavones slow the rise in prostate specific antigen (PSA) levels.20 PSA is protein produced by the prostate that can be measured in the blood, and is used as a marker of prostate cancer ...

Several nutrition and food ingredients are supposed to have beneficial effects, but precise cell biological mechanism has not been elucidated. Among food ingredients, polyphenols such as soy bean isoflavon genistein and wine resveratrol have been reported to have effects on lipid metabolism and cardiovacular diseases (1). In order to elucidate the effect of genistein on obesity, we cultured adipocyte and observed of genisten to lipid accumulation in cells. Triglyceride accumulation was suppressed by genistein when it was added at the time of differentiation but not when added after differentiation. Genistein is considered to suppress lipid accumulation by suppressing the differtiation of adipocytes.

3,4,7-Trihydroxyisoflavone C15H10O5 : Extrasynthese, 30 years of expertise in FLAVONOID Isoflavone reference materials and standards.

IRs have been extensively studied as mediators of insulins action in the periphery but also are found in the CNS (Raizada et al., 1988). With regard to the present study, insulin is known to alter stimulus-induced NE release and clearance of NE from extracellular space (Bhagat et al., 1981; Christensen, 1983; Shimosawa et al., 1992;Townsend et al., 1992). SK-N-SH cells possess IRs with a subunit composition similar to that reported for whole brain and neuronal tissue (Ota et al., 1988a; Raizada et al., 1988). In SK-N-SH cells, insulin binds to its receptors with aKd of ∼1 nM and stimulates 2-deoxyglucose uptake with an EC50 of 1 nM (Ota et al., 1988a).. IR is a membrane-bound tyrosine kinase whose activation leads to tyrosine autophosphorylation and the phosphorylation of IR-associated substrates. Consistent with this model, the tyrosine kinase inhibitor genistein, but not the inactive analog genistin, blocked the ability of insulin to enhance NE transport activity. One of the downstream ...

Soybeans are low in fat, zero cholesterol, fibre rich and protein packed, they are a key ingredient in a healthy diet. One of its components - genistein - has been shown to stimulate the skins production of collagen which in turn increases its elasticity and a youthful appearance.

This study aimed to explore a novel strategy for the simultaneous consumption of soluble soybean polysaccharides (SSPS) and insoluble genistein to improve the bioavailability of genistein and its prevention against obesity and metabolic syndrome in high-fat diet (HFD)-induced obese mice. C57BL/6J mice were fed a normal diet and HFD supplemented or not (n = 8) with SSPS (2.5%), genistein (0.5%) and ...

Background and Aim: It is known that oxidative stress plays an important role in the pathogenesis of diabetes. Consequently, use of antioxidant for treating and preventing complications of this disease is believed to be beneficial. Considering the antioxidant effects of soy isoflavones, this study was initiated to determine the effects ...

Fingerprint Dive into the research topics of Genistein, an isoflavone included in soy, inhibits thrombotic vessel occlusion in the mouse femoral artery and in vitro platelet aggregation. Together they form a unique fingerprint. ...

Biochanin A 7-O-(6-O-malonyl-beta-D-glucoside) | C25H24O13 | CID 5282148 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.

Data from epidemiological and human intervention studies have highlighted potential cardiovascular benefits of soy isoflavone-containing foods. In humans, genistein and daidzein are extensively metabolized after absorption into glucuronides and sulfate metabolites. However, limited data exist on isoflavone c

TY - JOUR. T1 - Genistein, an isoflavone included in soy, inhibits thrombotic vessel occlusion in the mouse femoral artery and in vitro platelet aggregation. AU - Kondo, Kazunao. AU - Suzuki, Yasuhiro. AU - Ikeda, Yasuhiko. AU - Umemura, Kazuo. PY - 2002/11/22. Y1 - 2002/11/22. N2 - Diet can be the most important factor that influences risks for cardiovascular diseases. Genistein included in soy is one candidate that may benefit the cardiovascular system. Here, we investigated the inhibitory effects of genistein on thrombotic vessel occlusion in the mouse femoral artery using a photochemical reaction, and in vitro platelet aggregation in whole blood measured by single platelet counting. Genistein (10 mg/kg), intravenously administered 10 min before the rose bengal injection, significantly prolonged the thrombotic occlusion time from 6.1±0.4 to 8.4±0.8 min (P,0.05). Genistein at doses higher than 30 μM significantly (P,0.01) inhibited in vitro platelet aggregation induced by collagen (1 and 3 ...

The green tea (Camellia sinensis) catechin, (-)-epigallocatechin-3-gallate (EGCG), has shown cancer-preventive activity in animal models. Previously, we have reported the bioavailability of EGCG in rats and mice. Here, we report that cotreatment of HT-29 human colon cancer cells with genistein (from soy) increased cytosolic EGCG by 2- to 5-fold compared with treatment with EGCG only. Inclusion of genistein, at non-cytotoxic concentrations, increased the growth inhibitory effects of EGCG against HT-29 cells (up to 2-fold at 20 μM genistein). Intragastric coadministration of EGCG (75 mg/kg) and genistein (200 mg/kg) to CF-1 mice resulted in an increase in plasma half-life (t1/2 148.7 ± 16.4 versus 111.5 ± 23.4 min) and exposure (AUC0→∞ 183.9 ± 20.2 versus 125.8 ± 26.4 μg/ml × min) of EGCG. Cotreatment with genistein also increased the maximal concentration (Cmax), 6 h exposure (AUC0→360 min), and t1/2 of EGCG in the small intestine by 2.0-, 4.7- and 1.4-fold, respectively, compared ...

Hepcidin, a peptide hormone that decreases intestinal iron absorption and macrophage iron release, is a potential drug target for patients with iron overload syndromes because its levels are inappropriately low in these individuals. Endogenous stimulants of Hepcidin transcription include bone morphogenic protein 6 (BMP6) and interleukin-6 (IL-6) by effects on mothers against decapentaplegic homolog (Smad)4 or signal transducer and activator of transcription (Stat)3, respectively. We conducted a small-scale chemical screen in zebrafish embryos to identify small molecules that modulate hepcidin expression. We found that treatment with the isoflavone, genistein, from 28-52 hours postfertilization in zebrafish embryos enhanced Hepcidin transcript levels, as assessed by whole-mount in situ hybridization and quantitative real-time reverse-transcriptase polymerase chain reaction. Genisteins stimulatory effect was conserved in human hepatocytes: Genistein treatment of HepG2 cells increased both ...

In a study of Antiproliferative and antiangiogenic activities of genistein in human renal cell carcinoma by Hiroto Sasamura, Atsushi Takahashi, Jinyang Yuan, Hiroshi Kitamura, Naoya Masumori, Noriomi Miyao, Naoki Itoh,Taiji Tsukamoto posted in The Official Journal of Societe Internationale D Urology, researchers found that Treatment with genistein for 48 hours inhibited cell proliferation in a dose-dependent manner and 100 μg/mL genistein inhibited it in a time-dependent manner. A dose of 50 μg/mL genistein clearly induced cell apoptosis. The vascular volume after implantation of the Millipore filter chamber containing RCC cells increased to threefold that without RCC cells. Genistein in the Millipore filter chamber significantly decreased the neovascularization induced by human RCC cells in vivo. and concluded that the results of this study demonstrated that genistein inhibited cell proliferation, induced apoptosis, and suppressed in vivo angiogenesis in human RCC cells. Genistein may be a ...

Looking for online definition of biochanin A in the Medical Dictionary? biochanin A explanation free. What is biochanin A? Meaning of biochanin A medical term. What does biochanin A mean?

Factors that up-regulate LDL receptor expression in normal and neoplastic cells include multiple growth factors, many hormones (such as insulin, glucagons, growth hormone) and steroid hormones, such as estrogens. Some authors have demonstrated, both in vivo and in vitro, a stimulation of LDL receptor mRNA and protein expression in hepatoma cell line HepG2 and in rat liver by a natural estrogen, 17β-estradiol, suggesting an estrogen regulation of hepatic LDL receptor expression [13, 15]. Recently, it has been showed that diets containing soy protein enhance the clearance of apoB-containing lipoproteins via LDL receptors expressed on the hepatocyte plasma membrane [7].. Dietary factors may regulate HMG-CoA reductase activity with implications for breast cancer development [16]. Kojima et al. have demonstrated that genistein itself and/or its metabolites may suppress hepatic lipid synthesis [20].. In this report, we show that LDL receptor and HMG-CoA reductase gene expression are affected by ...

BTG3/ANA/APRO4 has been reported to be a tumor suppressor gene in some malignancies. It constitutes important negative regulatory mechanism for Src-mediated signaling, a negative regulator of the cell cycle and inhibits transcription factor E2F1. We report that BTG3 is downregulated in renal cancer and that the mechanism of inactivation is through promoter hypermethylation. Quantitative real-time polymerase chain reaction (PCR) showed that BTG3 was downregulated in cancer tissues and cells. Genistein and 5-aza-2-deoxycytidine (5Aza-C) induced BTG3 messenger RNA (mRNA) expression in A498, ACHN and HEK-293 renal cell carcinoma (RCC) cell lines. Bisulfite-modified PCR and DNA sequencing results showed complete methylation of BTG3 promoter in tumor samples and cancer cell lines. Genistein and 5Aza-C treatment significantly decreased promoter methylation, reactivating BTG3 expression. Chromatin immunoprecipitation assay revealed that genistein and 5Aza-C increased levels of acetylated histones 3, 4, ...

Omar, SZ (2013) Effects of the phytoestrogen genistein on the development of the reproductive system of Sprague Dawley rats. Clinics, 68 (2). pp. 253-262. Omar, SZ (2013) Epigenetic analysis leads to identification of HNF1B as a subtype-specific susceptibility gene for ovarian cancer. NATURE COMMUNICATIONS, 4. pp. 1628-1638. Omar, SZ (2013) Hormonal changes causing rhinitis in pregnancy among Malaysian women. Journal of Laryngology and Otology, 127.9. p. 876. Omar, SZ (2013) Multiple independent variants at the TERT locus are associated with telomere length and risks of breast and ovarian cancer. 371-384. Raheem, I and Saaid, R and Omar, SZ and P.C., Tan (2012) Oral nifedipine versus intravenous labetalol for acute blood pressure control in hypertensive emergencies of pregnancy: A randomised trial. BJOG: An International Journal of Obstetrics and Gynaecology, 119 (1). pp. 78-85. PP, Khine and Vallikkannu, Narayanan and Omar, SZ and PC, Tan, (2010) Promethazine versus Metoclopramide for ...

Naturplus MenoControl menopause supplement.A potent combination of Soya Isoflavones with Sage, Brazilian ginseng and Royal Jelly, in a choice of 90, 180, 365 or 1000 count packs. Sizes available: 90, 180, 365 and 1000 capsule bags. QUALITY COVERAGE - At Naturplus we aim to provide you with high quality supplements at f

TY - JOUR. T1 - Differential regulation of protein tyrosine kinase on free radical production, granule enzyme release, and cytokine synthesis by activated murine peritoneal macrophages. AU - Kim, Young Ki. AU - Jang, Yoon Young. AU - Kim, Dong Hyun. AU - Ko, Hyun Hee. AU - Han, Eun Sook. AU - Lee, Chung Soo. PY - 2001/1/1. Y1 - 2001/1/1. N2 - The present study examined the regulatory effect of tyrosine kinase inhibitors (genistein, tyrphostin, and 2, 5-dihydroxycinnamate) on the free radical production, granule enzyme release, and synthesis of interleukin (IL)-8 and granulocyte macrophage-colony stimulating factor (GM-CSF) in murine peritoneal macrophages exposed to different stimulators [10 ng/mL of IL-1, 1 μg/mL of lipopolysaccharide (LPS), and 1 μM N-formyl-methionyl-leucyl-phenylalanine (fMLP)]. Protein tyrosine kinase (PTK) inhibitors attenuated the stimulated superoxide, hydrogen peroxide, and nitric oxide production in macrophages stimulated with IL-1, LPS, or fMLP N, ...

According to the statistics for 2018, cervical cancer is the fourth most common cancer in women worldwide, with 569,847 patients yearly [1]. Patients with cervical cancer who have progressed to an inoperable stage or have experienced recurrence receive widely used anti-cancer chemotherapy and platinum-based chemotherapy [2]. Cisplatin is a platinum-based chemotherapeutic agent that is widely used for the treatment of malignant tumors, such as cervical cancer, lung cancer, and ovarian cancer [3]. Cisplatin mainly induces cross-linking at the N7-position of guanosine, which modifies DNA to induce apoptosis and kill cancer cells [4]. However, the use of cisplatin as a cancer treatment has been limited owing to its serious side effects involving the kidney or hearing impairment and the emergence of resistant cancer cells [5]. In order to overcome anti-cancer drug resistance, high-dose chemotherapy with increasing dose combination therapy that combines several chemotherapy agents, and concurrent ...

This study aimed to explore a novel strategy for the simultaneous consumption of soluble soybean polysaccharides (SSPS) and insoluble genistein to improve the bioavailability of genistein and its prevention against obesity and metabolic syndrome in high-fat diet (HFD)-induced obese mice. C57BL/6J mice were f

The nuclear enzyme topoisomerase II, which is involved in replication, transcription, and probably repair of DNA, can be inhibited by a number of flavonoids. In conjunction with X-rays, three of these compounds were tested as to their effects on Reuber H35 hepatoma cells. In this combination, the isoflavone genistein, the flavone apigenin, and the flavonol quercetin caused an enhancement of radiation-induced cell death. This enhanced cytotoxicity was only observed when the flavonoids were applied following an irradiation treatment and is attributed to decreased repair of DNA radiation damage with a concomitant reduction of the rate of cell repopulation. Fractionated irradiations, given as five sequences of 3 Gy each over a period of 5 days, reduced the surviving cell population only by a factor of 20, whereas the continuous presence of genistein during radiation sequences resulted in a reduction of at least a factor of 10,000. Thus, these flavonoids not only seem to act as radiation enhancers ...

Two naturally occurring compounds from vegetables and soy slowed the spread of cancer cells in a new test-tube study. Researchers exposed breast and ovarian cancer cells to diindolylmethane (DIM), a compound that results from digesting vegetables such as broccoli and genistein, a soy isoflavone, and found that the compounds reduced the spread of cancer from cell to cell (metastasis) by 80% compared to cells that were not exposed. The doctors reported similar results using DIM and genistein on melanoma and prostate cancer cells, and noted that the amounts of the compounds are probably similar to high-dose supplements. DIM is also available as a supplement. Reference: ...

The results of the present study demonstrate that hypoxia induces HIF-1α protein stabilization as early as 10 minutes, with a maximum at 3 hours. Hypoxic signaling leading to HIF protein stabilization is mediated via the Shc/Ras signaling cascade, as demonstrated by overexpression of dnShc and dnRas in HUVECs. Hypoxia-induced tyrosine phosphorylation of Shc seems to occur as one of the earliest events, peaking at 10 minutes after hypoxic exposure, followed by subsequent Shc-dependent activation of Ras, which showed maximal activity under the influence of hypoxia at 30 minutes.. Previous studies have suggested that hypoxic signaling leading to HIF expression involves activation of tyrosine kinases, because induction of HIF-1 was inhibited by pretreating cells with the tyrosine kinase inhibitor genistein.14. However, a specific tyrosine kinase initiating the signaling events leading to HIF stabilization has not been identified. It has been shown that the transformation of cells with the ...

This study was done to evaluate the antioxidant activity of Pueraria lobata. Pueraria is known as kudzu in Japan and gegen in China. This study was done on diabetic rats. The chemicals included puerarin (and variants,) daidzin, genistin, daidzein and genistein. The rats were given pueraria roots (500 mg/kg extract) containing 50 mg/kg puerarin, the […]. View Post ...

How soy or Glp-2 could enable cancer by lengthening the bowel tract… If Glp-2 helps with malabsorption in the bowel caused by innate or surgical shortening of the bowel tract, and… If Glp-2 is similar to soy genistein… If Glp-2 helps lengthen, and regrow the bowel tract… If a longer bowel tract is associated with cancer… If soy lengthens the bowel tract… If Coppers like licorice root & coffee shorten the bowel tract… Rough notes: Observations on soy( genistein) on length of bowel tract, & how shorter bowel tracts get

A study carried out by the WHO in 50 countries found that hormone related cancers in the West were from 10 to 20 times higher than that found in Asian countries. The low level has been ascribed to their diet which is high in phytochemicals. The principal ones are the isoflavones diadzein and genistein, found in all soy products, including soymilk, tofu and miso. Recently two more such natural cancer supplements have been discovered... biochanin and formononetin. These are not found in soy but in chickpeas, lentils, mung beans and red clover. To get the full benefit of these isoflavones 100gm of soy, chickpeas etc should be eaten three times per week ...

This human study shows that the isoflavone aglycone genistein penetrates significantly better into the skin when it is formulated in a liposomal preparation. The corresponding isoflavone glycoside genistin is not hydrolyzed to the biologically active aglycones upon topical application.

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DENG Wei-min , ZHANG Peng , HUANG Hai , SHEN You-gao , YANG Qin-hua , CUI Wei-li , HE Yang-shu , WEI Song , YE Zhu , LIU Fang , QIN Ling Journal of bone and mineral metabolism 30(5), 517-524, 2012-09-30 医中誌Web 参考文献47件 ...

Proteases Inhibitors on signaling pathway are available at Adooq Bioscience. Check Proteases pathway , inhibitors reviews and assay information.