TLR2/NF-kB reporter cell line/TLR2 cell line can be used to monitor the TLR2 signaling pathway activity. Screen for activators or inhibitors of the TLR signaling pathway.
... s are fused to DNA fragments from a test gene or promoter. Reporter genes must be easily measured enzymatically or visually. Two common reporter genes are Β-galactosidase, green fluorescent protein (GFP) and yellow fluorescent protein (YFP). Β-gal is measured enzymatically using either X-gal (which Β-gal cleaves into a blue compound) or ONPG (which Β-gal cleaves into a yellow compound). GFP and YFP can be measured enzymatically, but are more easily measured visually because they fluoresce brightly.. ...
Gentaur molecular products has all kinds of products like :search , SBI \ Bioluminescent Imaging Lentiviral Dual Reporter MSCV-RFP-Luciferase pre-packaged virus \ BLIV300VA-1 for more molecular products just contact us
Gentaur molecular products has all kinds of products like :search , SBI \ Bioluminescent Imaging Lentiviral Dual Reporter MSCV-GFP-Luciferase pre-packaged virus \ BLIV301VA-1 for more molecular products just contact us
Regulation of gene expression can be achieved by fusing miRNA binding sites into the 3UTR of a GOI. In case a referring shRNA miR is endogenously present or co-expressed, the GOI is knocked down. Strength of regulation thereby depends on binding site properties. We are able to tune gene expression linearly over a broad range. This is a first proof of principle for various miRNA-mRNA interaction in vitro. Therefore, we transfected HeLa cells in principle with our pSMB_miTuner Plasmid HD3 . It turned out, that no obvious effect of different binding sites on reporter gene expression could be measured (data not shown). We assume that the RSV promoter driving the shRNA miR is too weak for tight regulation of the referring binding site behind the GOI. Only if a sufficient amount of shRNA miR binds to its target, translation is significantly repressed. Thus, we expressed the shRNA miR from a separate plasmid which was always co-transfected with the original tuning construct. The reporter genes - i. e. ...
The Fence Builders has recently opened its newest office and it promises to be the go-to place for all fencing needs. While being in the business for five years, the. ...
Today, the website of Mini PC News introduces the Best Gaming Mini PCs Under 300 Dollars 2017. The spokesman for this website says, "The experts at budget Mini PCs are. ...
Easily study the dynamics of cellular processes with SBIs Cyto-Tracers™. Leverage SBIs high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors-available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The ER-Golgi Cyto-Tracer, pCT-Secretory-GFP (CMV) fuses a secretory consensus tag to copGFP, resulting in copGFP-labeling of the ER and Golgi. The copGFP-fusion is expressed from the CMV promoter, for strong expression in common cell types such as HeLa, HEK293, and HT1080 ...
Easily study the dynamics of cellular processes with SBIs Cyto-Tracers™. Leverage SBIs high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors-available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Nucleus Cyto-Tracer, pCT-H2B-GFP (MSCV) fuses an H2B tag to copGFP, resulting in copGFP-labeling of the nucleus. The copGFP-fusion is expressed from the MSCV promoter, for strong expression in hematopoietic and stem cells ...
Jill Abramson, executive editor of the New York Times, gave a frank overview of the evolving role of the newsroom as she described how the Times is blending traditional separations between print and web ...
The LightSwitch 3´UTR Reporter Collection from Active Motif include over 12,000 3´UTRs available as transfection-ready luciferase reporter vectors for use in reporter assays.
We were contacted recently with the following request which we are unable to fulfill:- ...................................................................... Hi, I am studying cis-regulation of a gene encoding nuclear protein. I am using beta-gal to show the expression pattern of this gene. But the result is not so good because beta-gal is expressed in cytoplasm, not nuclearly. Do you know any nuclear reporter gene I can use? Thank you very much! Zhe Han zhan at umich.edu ...................................................................... If you are able to help please contact Zhe directly. Corniche.. the On-line life science resource. Free to use searchable database conatining 50,000 products from many sources large and small. http://www.corniche.com ...
"Reporter genes have been used for several decades to study regulation of gene... *Hitra in zanesljiva dostava, plačilo tudi po povzetju.*
Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity ...
Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity ...
Deport the Middletons to Sandwich Islands: campaign gathers momentum : London A TV presenters quip about deporting problem families to the far flung archipelago has morphed into an exciting new project Sky News reporter Colin Braziers suggestion about sending 120000 miscreant families each costing the taxpaye
CBS Evening News reporter Steve Hartman took a deeper look into how his two young kids were computing the lyrics of country songs in their developing brains as
Project no. 911388 outlined 3 major aims to be addressed; Development of novel optical reporter systems, implementation of these reporters and probes in optical imaging of cancer and in translational therapeutic imaging. Results are very encouraging with publications resulting in prestigious journals. Gjerdrum et al. (2010) employed the use of novel imaging the impact of shRNA knockdown in vivo, while novel time-domain technique was described to image fluorescent reporters in vivo (Rosland et al. 2009, Torsvik et al. 2009). Combination of novel imaging strategy and near-infrared labeled mAbs and dextrans permitted visualization of drug uptake (McCormack et al. 2009), while Wang et al. (2010), Karlsen et. al. (2012) and 2 further manuscripts (McCormack et al. Submitted 2011/2) detail novel imaging strategies and reporters for in vivo imaging of patient xenografts. Translational application of cancer models and imaging are detailed in Erikstein et al. (2010), McCormack et al. (2010), and in ...
PrimCells is the reliable choice for your primary cell needs. We carry a variety of high quality primary cells, reporter cell lines and protein factors. Our uniquely design molecular tools provide you the most cost effective and reliable tool sets that can empower your research.
EPA-induced translational up-regulation of reporter genes fused to BRCA1 mRNAb 5′UTR is dependent on the presence of tandem uORFsThe plasmid encoding for F-lu
Available with Biological Response Genes AdenoExpress™ Available with Biological Response Genes Cytokines GM-CSF IFN-a IFN-g IL-2 IL-12
EXPRESS: If we want to use the regulatory aspects of NBB4 ethylene metabolism in a biosensor, we need to incorporate a reporter gene with strong, detectable output into our circuit. Generating such a reporter gene was the focus of EXPRESS, where we used error prone PCR on an existing blue iGEM chromoprotein amilCP to generate amilCP mutants with altered spectral characteristics ...
Method of detection in vitro of a target substance in a sample comprising the labelling of said substance with a reporter gene and with the sequences necessary for the expression of said reporter gene in vitro ...
E2HSA exhibits GLP-1 receptor activating efficacy in NIT-1 cells. NIT-1 cells transiently transfected with Peak12 RIP-CRE 6x Luciferase reporter gene plasmid we
Community testing a must, she says State Rep. Liz Miranda is on the mend after testing positive for COVID-19 on May 1. The 39-year-old lawmaker, who has represented the 5th Suffolk District since Jan. 2019, talked to the Reporter this week about her experience with the virus, health disparities in Roxbury and Dorchester, and the importance of community testing. Of the 20 of
Amused at a couple of things in the Glen Rose Reporter article of June 2, 2016 that deals with Luminants not having paid the entire property tax ....
Given there was another eye-witness who wasnt arrested, I would guess that the area wasnt secure and was open for others to use, so that at least would be out as far as justifications for the arrest.. The statement made by that eyewitness would also seem to contradict the aggressive narrative that the arrest was based on, though its possible that the SS had a different idea as to what constituted too close that differed from the eyewitness and the arrested reporters.. The fact that yelling questions was categorized as causing a disturbance is all sorts of questionable on its own, and the fact that it was included in the complaint does not bode well for the rest of the thing, especially given the other two points above. ...
Hottie Mexican reporter Ines Sainz described New York Jets catcalls as she was working on a story in their locker room as grotesque. It mustnt happen again, she told the New... Sports News Summaries. | Newser
Im a reporter in the Greensboro, N.C. area and Im working on an article about tinnitus. Im hoping to educate young people about the dangers of loud...
Bio-Synthesis offers a complete array of fluorescent reporters and quenchers. Our product offering includes common dye modifiers such as 6-FAM, HEX and TET, as well as exotic modifications
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A recent report from undercover reporters reveals a Ticketmaster scalping scandal and InvestorPlace has a few highlights about it.
After almost 10 months of steadily increasing neuro symptoms, KVUE reporter Jenni Lee is finally beginning to feel like herself again.
BELLEFONTE Georgene Searfoss walked out of the YMCA, in the shadow of the Centre County Courthouse, when she spotted a reporter and asked, Did he get his sentence?
OV-IA82Δ113 and OV-IA82Δ116 viruses were constructed by infecting OFTu cells (in T25 flasks) at a multiplicity of infection (MOI) of 1.0 with wild type OV-IA 82 for 3 hours and subsequently transfecting the cells with 10 μg of pSVP-113LF-EGFP-113RF and pSVP-116LF-EGFP-116RF transfer vectors by standard in vivo recombination protocols [28, 29]. Transfections were carried out using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions.. Viruses were harvested 48 h pi by scraping infected/transfected OFTu cells into sterile 15 ml conical tubes. The cell suspensions were vortexed, frozen/thawed 3 times, and then centrifuged at 1000 rpm, for 10 min at 4°C (Eppendorf Centrifuge 5810R, 15 amp version, Hamburg, Germany). The supernatant (viruses) were transferred into 2 ml cryogenic vials (Corning, NY, USA) and stored at -80°C for future use.. In order to select and purify recombinant viruses, limited dilution and plaque assays were performed. 3 ×104 of ...
Signaling through the Notch1 receptor is essential for the control of numerous developmental processes during embryonic life as well as in adult tissue homeostasis and disease. Since the outcome of Notch1 signaling is highly context-dependent, and its precise physiological and pathological role in many organs is unclear, it is of great interest to localize and identify the cells that receive active Notch1 signals in vivo. Here, we report the generation and characterization of a BAC-transgenic mouse line, N1-Gal4VP16, that when crossed to a Gal4-responsive reporter mouse line allowed the identification of cells undergoing active Notch1 signaling in vivo. Analysis of embryonic and adult N1-Gal4VP16 mice demonstrated that the activation pattern of the transgene coincides with previously observed activation patterns of the endogenous Notch1 receptor. Thus, this novel reporter mouse line provides a unique tool to specifically investigate the spatial and temporal aspects of Notch1 signaling in vivo. ...
The assessment of how many cells have been successfully transfected or transduced in a cell population is a basic and critical evaluation parameter in many cell and molecular biology labs. Commonly, the cells of interest are transfected or transduced with a construct that results in the expression of a fluorescent protein (FP) reporter, such as GFP.
BioAssay record AID 95279 submitted by ChEMBL: Cellular activity was measured by an IL-2 luciferase reporter gene assay on a Jurkat human T cell line activated by anti-CD28 and anti-TCR antibodies.
The Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay allows you to use firefly luciferase, Nanoluc luciferase, or both, as primary reporters. This article provides information on when to use firefly and when to use Nanoluc luciferase as a primary reporter, and how both reporters can be used together to measure two biological responses in the same sample.
riyer at rascal.med.harvard.edu wrote: : I recd. so many requests from people who did not get my earlier post that I : decided to save time by re-posting it. At the outset I would like to state : that I am only a satisfied user of the above vectors and am not associated : with the company that markets them. And now here comes a readable version (with ,80 chars/line) :-) : Hi netters, I would like to report the availability of a set of : reporter gene vectors that have some distinct advantages over those : currently in use for the purpose of analysis of eukaryotic promoters : & enhancers. The vectors , SV40-Syncat, Syncat I, Syncat II, : SV40-pFlash, pFlash I and pFlash II, are as their names suggest : CAT & Luciferase (Luc) reporter gene vectors. What distinguishes : them from other vectors in this category is that these vectors produce : near-zero background. This feature is due to the fact, that they use a : modified SV40-t-intron as the donor for the poly-adenylation signal. : The wild type ...
A signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence includes steps of: contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each reporter probe including a signal region and an oligonucleotide sequence which is complementary to and capable of forming a stable hybrid with the analyte homopolymeric region to form an analyte:reporter probe hybrid; and forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions. The analyte:reporter probe hybrid may formed prior to contacting the analyte target sequence with the capture probe, so the result of contacting the analyte target sequence with the capture probe results in formation of an analyte:reporter probe:capture probe complex. The analyte:capture probe hybrid may be immobilized on a solid generally planar surface in an array format. Multiple reporter probes may form triple
The synthetic poly(A) signal in the pCAT3 vector and several other Promega vectors is placed upstream of the reporter to eliminate background and vector dependent reporter signal. Basically, all vectors have cryptic transcription factor binding sites that will cause the reporter to be transcribed. By putting in a polyadenylation signal, these transcripts should no longer contain the reporter gene because it is spliced off and will not cause artificial signal. I hope this is clear. If you have any additional questions, please feel free to contact me at [email protected] ...
Recombineering is a genetic engineering tool that enables facile modification of large episomal clones, e.g. BACs, fosmids. We have previously adapted this technology to generate, directly from fosmid-based genomic clones, fusion gene reporter constr
Anew detection mechanism for the control of successful siRNA delivery to cells or tissue is introduced using a siRNA-based probe that is capable of inducing a ...
Thermo Scientific™ Gaussia-Dura Luciferase Reporter Assay Vectors pTK-Gaussia-Dura Luc Vector Thermo Scientific™ Gaussia-Dura Luciferase Reporter...
The EliKine™ Human IL-1β ELISA Kit is one of the latest scientific research products from Abbkine Scientific Co. Ltd. The company recently announced the launch of the product, designed to enhance scientific research processes.. The IL-1β is a member of the interleukin 1 cytokine family, produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). Interleukin 1β is an important mediator of the inflammatory response, involved in several cellular activities, including cell proliferation, differentiation, and apoptosis.. Interleukin 1β Elisa kit has human reactivity, employing a two-site sandwich ELISA to quantitate IL-1β in samples. The IL1B Elisa kit uses colorimetric method of detection, with the suitable samples types being Cell culture supernatants, other biological fluids, Plasma, and Serum.. The components of the kit include:. ...
Watch the introductory video to learn more about our database of embryonic development for stem cell research and regenerative medicine ...
The Global CM & CRM Devices Sales Market Report 2021 is a professional and in-depth study on the current state of the CM & CRM Devices Market. This report studies sales (consumption) of CM & CRM Devices in Global market, especially in North America, Europe, China, Japan, Southeast Asia and India, focuses on top players in these regions/countries, with sales, price, revenue and market share for each player in these regions, covering North America, Europe, China, Japan, Korea, Taiwan focuses on top manufacturers in global market, with production, price, revenue and market share for each manufacturer, covering Medtronic PLC , St. Jude Medical, Inc. , Boston Scientific Corporation , GE Healthcare , Philips Healthcare , Biotronik Se & Co., Kg , Cardiac Science Corporation , Livanova PLC , Mortara Instrument, Inc. and Schiller AG.. Request a Sample copy of this research @ http://www.reportsweb.com/inquiry&RW0001513144/sample. Market Segment by Regions, this report splits Global into several key ...
Dc3 is a carrot lea-class gene belonging to a small gene family that encodes Dc3 and Dc3-like RNA sequences. We have examined the responsiveness to water deficit and abscisic acid (ABA) of the promoter/enhancer complex of Dc3 fused to a beta-glucuronidase (GUS) reporter gene in vegetative cells of transgenic tobacco. In 56-d tobacco, GUS expression in leaves increased about 200-fold during a 3-d d ...
Currently about 1,500,000 people die of AIDS and its complications each year. Many die of fungal infections - how many is not clear because of a lack of access to excellent diagnostic tests.. In Seattle, over 60 experts from major health agencies, including the World Health Organisation, UNAIDS, Bill and Melinda Gates Foundation, Clinton Health Access Initiative, Office for Global AIDS Coordination, Centers for Disease Control The Global Fund - and representatives from over 30 countries -- gathered to align on efforts to improve management of fungal infections. Delegates highlighted the lack of diagnostics and access to treatments, including the oldest antifungal drugs, as major hurdles to improvements in many developing countries.. A key output of the meeting based on a preliminary model created to analyze the costs associated with treatment and care of fungal infections in Africa, showed that early screening and treatment, before meningitis or pneumonia develop, is cost effective. If ...