Complete information for IGL gene (Protein Coding), Immunoglobulin Lambda Locus, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Somatic hypermutation (SH) of V(D)J rearrangements at the IGH and IGL loci diversifies the IG repertoire during the germinal center response. SH is absolutely dependent on the enzyme activation induced cytidine deaminase (AID) that initiates the SH process by deaminating C nucleotides in ssDNA. Mutations from G and C are thought to occur as a result of strand symmetrical deamination of C by AID on the coding and non-coding strands respectively. Mutations from A and Tare introduced in a strand biased way during error prone repair of the AID induced lesion. SH is linked to transcription and it has been proposed that bidirectional transcription across V(D)J rearrangements occurs in activated and quiescent B cells and that it is co-opted to facilitate the accessibility of the two DNA strands by regulating accessibility of single stranded DNA to AID. We have developed a quantitative method to study directional transcription. Our method controls for differences in efficiency and specificity of reverse ...
TY - JOUR. T1 - Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasing point mutation. AU - Kajita, Masamichi. AU - Okazawa, Takahiro. AU - Ikeda, Mika. AU - Todo, Kagefumi. AU - Magari, Masaki. AU - Kanayama, Naoki. AU - Ohmori, Hitoshi. PY - 2010/9/1. Y1 - 2010/9/1. N2 - The chicken B cell line DT40 undergoes hypermutation of immunoglobulin variable region (IgV) genes during culture, thereby constituting an antibody (Ab) library. We previously established an in vitro Ab generation system using an engineered line DT40-SW whose hypermutation machinery can be switched on and off. Abs for various antigens (Ags) can be obtained from the DT40-SW library and the specificity of the Ag-specific clones can be stabilized by stopping hypermutation. Furthermore, the affinity of obtained monoclonal Abs (mAbs) can be improved through further mutation followed by selection, a process analogous to affinity maturation that occurs in vivo. Although gene ...
Immunoglobulin light chain gene translocations in non-Hodgkins lymphoma as assessed by fluorescence in situ hybridisation.: In non-Hodgkins lymphoma (NHL), th
Fingerprint Dive into the research topics of Preferential linkage of bcl-2 to immunoglobulin light chain gene in chronic lymphocytic leukemia. Together they form a unique fingerprint. ...
Polymerase Chain Reaction (PCR). PCR assays are routinely used for the identification of clonal B-cell populations. These tests amplify the DNA between primers that target the conserved framework (FR) of the variable (V) regions and the conserved joining (J) regions (IGL Tube). These conserved regions lie on either side of an area within the V-J region where programmed genetic rearrangements occur during maturation of all B and T lymphocytes. The antigen receptor genes that undergo rearrangement are the immunoglobulin heavy chain andlight chains in B-cells, and the T cell receptor genes in T-cells. Each B- and T-cell has a single productive V-J rearrangement that is unique in both length and sequence. Therefore, when DNA from a normal or polyclonal population is amplified using DNA primers that flank the V-J region, a bell-shaped curve (Gaussian distribution) of amplicon products within an expected size range is produced. This Gaussian distribution reflects the heterogeneous population of V-J ...
Monoclonal antibodies (mAbs) can be potent and highly specific therapeutics, diagnostics and research reagents. Nonetheless, mAb discovery using current in vivo or in vitro approaches can be costly and time-consuming, with no guarantee of success. We have established a platform for rapid discovery and optimization of mAbs ex vivo. This DTLacO platform derives from a chicken B cell line that has been engineered to enable rapid selection and seamless maturation of high affinity mAbs. We have validated the DTLacO platform by generation of high affinity and specific mAbs to five cell surface targets, the receptor tyrosine kinases VEGFR2 and TIE2, the glycoprotein TROP2, the small TNF receptor family member FN14, and the G protein-coupled receptor FZD10. mAb discovery is rapid and humanization is straightforward, establishing the utility of the DTLacO platform for identification of mAbs for therapeutic and other applications.
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The chicken B cell line DT40 is a tool that is uniquely situated to study the function of genes due to its high rate of homologous recombination. As any tool is only as good as the resources behind it, the recent boon and continuing output of available genomic information has only widened the possibilities for the DT40 community. Besides the release of the chicken genome, the public databases are expanding rapidly with a wealth of experimentally produced data including various chicken cell specific ESTs, full-length cDNAs, non-coding RNAs and expressed SAGE tags. In addition, many laboratories have also taken it upon themselves to set up a web presence freely sharing and distributing information, program applications and software such as the DT40 website. Of course, the standard bearers in this are the institutes that have led the way in development of bioinformatics tools for sequence information, comparative genomics and proteomics such as The National Center for Biotechnology Information ...
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Human ATMIN full-length ORF ( AAH02701.1, 1 a.a. - 667 a.a.) recombinant protein with GST-tag at N-terminal. (H00023300-P01) - Products - Abnova
Genes encoding the different subunits of the IMMUNOGLOBULINS, for example the IMMUNOGLOBULIN LIGHT CHAIN GENES and the IMMUNOGLOBULIN HEAVY CHAIN GENES. The heavy and light immunoglobulin genes are present as gene segments in the germline cells. The completed genes are created when the segments are shuffled and assembled (B-LYMPHOCYTE GENE REARRANGEMENT) during B-LYMPHOCYTE maturation. The gene segments of the human light and heavy chain germline genes are symbolized V (variable), J (joining) and C (constant). The heavy chain germline genes have an additional segment D (diversity ...
Garver, F.A.; Chang, L.; Mendicino, J.; Isobe, T.; Osserman, E.F., 1975: Primary structure of a deleted human lambda type immunoglobulin light chain containing carbohydrate: protein Sm lambda
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Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
Immunoglobulin secretion by plasma cells infiltrating synovial membranes is a prominent feature of RA. Previous analyses of a cDNA library generated from synovium of RA patient BC revealed immunoglobulin κ light chain transcripts with extensive somatic mutation, frequent N region addition, and unexpected variation in the lengths of CDR3 regions which form the center of the antigen binding site. To determine if these characteristics are present in other individuals, we performed reverse transcription-polymerase chain reaction amplification and sequenced ≥10 Vκ-containing amplicons from nine tissue samples: synovia of three individuals with long-standing RA (including patient BC), PBLs of two of these individuals, and PBLs or splenocytes of four normal individuals. Increased levels of somatic mutation in PBLs appeared to correlate with increased age, which may reflect accumulation of circulating memory cells and/or decreased bone marrow production of naive B lymphocytes. Two of three RA ...
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Amyloid fibrils and partially unfolded intermediates can be distinguished serologically from native amyloidogenic precursor proteins or peptides. In this regard, we previously had reported that mAb 11-1F4, generated by immunizing mice with a thermally denatured variable domain (VL) fragment of the h …
Addresses: Pilstrom L, Uppsala Univ, BMC, Immunol Programme, Dept Cell & Mol Biol, POB 596, S-75124 Uppsala, Sweden. Uppsala Univ, BMC, Immunol Programme, Dept Cell & Mol Biol, S-75124 Uppsala, Sweden.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-14 ...
A patient is presented in whom the amyloid component of an intracerebral amyloidoma has been purified and characterized by amino acid sequence analysis. The material originated from an autopsy of a 76-year-old man who 15 years earlier had been operated for an intracerebral amyloid tumour. The tu …
Principal nameIGKV1-5 antibodyAlternative names for IGKV1-5 antibodyIg kappa chain V-I region HK102SwissProt IDP01602 (Human)Gene…
Characterization of the immunoglobulin (Ig) light (L) and heavy (H) chain genes from the Siberiansturgeon, Acipenser baeri, revealed an Ig structure simhar to the IgM elass of mammals. The serumforms of the sturgeon Ig are tetrameric and dimeric.. The sturgeon IgL gene organization is also of the translocon type, with several variable (V)segments followed by some joining (J) segments and the constant (C) exon indicate that theformation of IgL multicluster organisations in teleosts and elasmobranchs are two separateevolutionary events. The sturgeon VL segments all belong to a single family with some 20 members.Light chain variability is generated by random rearrangement of the VL and J segments. The sturgeon IgL is classified as κ like based on homologies of the VL sequence, and similarities of the recombination signal sequence.. The genomic organization of the IgH locus in sturgeon is of the translocon type. withmultiple V gene segments preceeding several D segments, a number of J segments ...
(A) CBE at the Igκ locus in B cell development. Purified DNA from sorted subpopulations of B cells from wild-type Balb/c mice was subjected to LMPCR to detect
TY - JOUR. T1 - The analysis and quantification of a clonal B-cell response in a hyperimmunized anti-D donor. AU - Dohmen, S. E.. AU - Verhagen, A.. AU - De Groot, S. M.. AU - Stott, Lisa Marie. AU - Urbaniak, Stanislaw. AU - Van Der Schoot, C. E.. PY - 2006/5. Y1 - 2006/5. N2 - Healthy volunteers are hyperimmunized with RhD-positive red cells in order to obtain plasma containing high titres of anti-D immunoglobulin, which is used for the prevention of haemolytic disease of the fetus and newborn. We analysed the anti-D immune response in a donor who had been hyperimmunized for 7 years and who showed declining anti-D titres despite re-immunization. A phage display library representing the complete immunorepertoire and a second library representing the IGHV3 superspecies family genes (IGHV3s) repertoire in the donor were constructed and analysed. A clonal Ig-gene rearrangement was quantified in the peripheral blood by limiting dilution polymerase chain reaction (PCR) All RhD-binding phages from ...
So why do I think all of this is predatory in nature? I receive this bill every 20 days, the same bill, and my husband and I make the required phone calls to the medical facility and the primary insurance company. So far neither institution has been able to resolve this claim. If we failed to make these phone calls every 20 days this bill, in all likelihood, would go to collection and could balloon to twice what it is now with fees and interest. That being said, in this case the medical facility actually owns the collection agency that these bills go to so they not only get to collect the amount of the bill, but the fees and interest as well. That doesnt sound shady at all does it?. What most people dont know or dont care to take advantage of is that if you call the medical facility that has sent you a bill to make payment arrangements or to dispute the charges they, in most circumstances, will not send you to collection; however, you have to call them EVERY TIME you receive the same bill. ...
TY - JOUR. T1 - Kappa light chain-associated Fanconis syndrome. T2 - Molecular analysis of monoclonal immunoglobulin light chains from patients with and without intracellular crystals. AU - Déret, Sophie. AU - Denoroy, Luc. AU - Lamarine, Marc. AU - Vidal, Ruben. AU - Mougenot, Béatrice. AU - Frangione, Blas. AU - Stevens, Fred J.. AU - Ronco, Pierre M.. AU - Aucouturier, Pierre. PY - 1999/5/17. Y1 - 1999/5/17. N2 - Plasma cell dyscrasias may be responsible for Fanconis syndrome, due to the toxicity of a free monoclonal kappa light chain toward kidney proximal tubules. Eight cases of Fanconis syndrome were analyzed. We compared the structures of VκI variability subgroup V domains from five cases of Fanconis syndrome and one myeloma without renal involvement. Among Fanconi cases, four putative structures were obtained after molecular modeling by homology, and the other had previously been refined by X-ray crystallography. The complete sequences of one VκI, one VκIII and N-terminal ...
Immunoglobulin light chain amyloidosis (AL) is characterized by a limited clonal expansion of plasma cells and amyloid formation. Here, we report restriction in the diversity of VL gene usage with a d
Note Added in Proof to appear with the article: A Homologue of the Rabbit Kappa Chain Allotype b9 on IgG from a Cottontail Rabbit by Simonetta Landucci Tosi, Rose G. Mage, and Roberto M. Tosi appearing on page 679:. Extensive studies of the phylogeny of rabbit immunoglobulin light chain markers have recently been completed by W. van der Loo, C. Hamers-Casterman and R. Hamers, and reported in a brief communication entitled, Phylogeny of the rabbit immunoglobulin light chain markers: Distribution of determinants associated with rabbit b-locus in different lagomorph families published in Arch. Internat. Physiol. Biochim. 89, 54, 1976. A detailed report entitled, Investigations of the origin of the b-locus polymorphism of the rabbit kappa light chain is contained in the D. Sc. thesis, Vrije Universiteit Brussels by Dr. W. van der Loo, 1975. Their observations are generally in accord with ours.. ...
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