RNA INTERFERENCE MEDIATED INHIBITION OF FOS GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) - diagram, schematic, and image 148 ...
RNA INTERFERENCE MEDIATED INHIBITION OF FOS GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) - diagram, schematic, and image 26 ...
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex, at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation (By similarity). In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum (By similarity).
Stimulation of pancreatic β-cells with glucose activates the protein kinases B-Raf and extracellular signal-regulated protein kinase that participate in glucose sensing. Inhibition of both kinases results in impairment of glucose-regulated gene transcription. To analyze the signaling pathway controlled by B-Raf, we expressed a conditionally active form of B-Raf in INS-1 insulinoma cells. Here, we show that stimulation of B-Raf strongly activated the transcription factor AP-1 which is accompanied by increased c-Jun and c-Fos promoter activities, an upregulation of c-Jun and c-Fos biosynthesis, and elevated transcriptional activation potentials of c-Jun and c-Fos ...
... - Its been so long since Ive actually posted any of my FOs on craftster. I guess I just fell into a funk for a while. Anyway, here a couple
The immediate early gene c-fos is one of the most studied genes in the CNS as a marker for neuronal activation (Edwards et al.,1999; Abraham and Kovacs,2000; Konkle and Bielajew,2004). It is thus generally believed that activation of a neuronal system with c-fos expression in the brain is a hallmark for reflecting the functional status of a discrete brain structure. The c-fos belongs to the family of immediate-early transcription factor genes that are believed to function in coupling short-term signals elicited by extracellular to long-term changes in cellular phenotype by orchestrating changes in target gene expression (Curran and Morgan,1995). However, the expression of c-Fos, which is normally low, can be increased by a number of pharmacological, physiological, and behavioral manipulations (Morgan and Curran,1989; Herrera and Robertson,1996). Therefore, the measurement of c-Fos protein levels, the product of c-fos gene activation, has been used as a marker for activated neurons (Hoffman et ...
Excessive extracellular glutamate triggers delayed neuronal death by promoting the influx of calcium into cells by activating N-methyl-D-aspartate (NMDA) or non-NMDA glutamate receptors. [4] Glutamate receptor activation, in turn, stimulates expression of rapidly induced transcriptional activators known as the immediate-early genes. These genes initiate a complex cascade of events that transduct extracellular signals into alterations of cellular functions by regulating target gene expression (late-response genes). [5-7] The immediate-early gene c-fos is rapidly and transiently induced in neurons within the hippocampal formation of the brain after seizures, hypoxia, and global ischemia through glutamate-mediated NMDA and non-NMDA receptor activation. [8-10] The protein product of c-fos mRNA, FOS, modulates transcription of several late-response genes, such as p53, heat-shock protein, bcl-x, tyrosine hydroxylase, and opioid peptides. [10-12] Some of the late-response genes expressed after c-fos ...
Expression of cellular oncogenes was studied in a T cell hybridoma that undergoes cytolytic activation when stimulated by specific antigen or by anti-Thy-1 antibody. The activation occurs without induction of hybridoma proliferation, providing a model to examine oncogene expression during functional differentiation of lymphocytes. We found that c-fos and c-ets-1 mRNAs were transiently induced at high levels in the hybridoma 30 min and 4 h after stimulation, respectively. c-myc and c-ets-2 oncogenes were constitutively expressed in the hybridoma and their mRNA levels were unaffected during 4 h of stimulation, although c-myc expression was reduced in the later stage of stimulation. Inhibitors of T cell activation, cyclosporin A and anti-LFA-1 antibody, blocked the induction of c-fos and c-ets-1 mRNAs without reducing the levels of c-myc and c-ets-2. The results indicate that the functional activation of the CTL hybridoma is associated with induction of c-fos and c-ets-1 genes. ...
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Edelleenkin olen tosiaan täällä. Ennen Lassin syntymää valmistuneet neuleet ovat edelleen kuvaamatta ja uusia ei ole syntynyt. Olen sen sijaan keskittynyt kotimme pieniin ihmeisiin, Niiloon ja Lassiin. Yes, still here. No, I havent taken pictures of the FOs I finished before the baby was born, and, no, I havent finished any new ones. I have…
OBJECTIVE To compare the mechanotransduction caused by cyclic and static mechanical strains in human periodontal ligament fibroblasts (hPDLFs) cultured under identical conditions. MATERIALS AND METHODS hPDLFs, originating from the same donors, were exposed either to cyclic or to static tensile strain using specially designed devices and under identical culture conditions. Activation of all members of mitogen-activated protein kinases (MAPKs) was monitored by western immunoblot analysis. Expression levels of immediate/early genes c-fos and c-jun were assessed with quantitative real-time polymerase chain reaction. RESULTS Time course experiments revealed that both types of stresses activate the three members of MAPK, that is ERK, p38, and JNK, with cyclic stress exhibiting a slightly more extended activation. Further downstream, both stresses upregulate the immediate/early genes c-fos and c-jun, encoding components of the activator protein-1 (AP-1), a key transcription factor in osteoblastic ...
We report here that levels of c-fos mRNA in GnRH neurons are elevated significantly coincident with the first increase in LH levels at the time of an E- and P-induced LH surge in female rats. Similarly, Fos protein increases in GnRH neurons near the time of the LH surge in rats (Lee et al., 1990a; Hrabovszky et al., 1995; Wang et al., 1995), mice (Wu et al., 1992), hamsters (Berriman et al., 1992;Doan and Urbanski, 1994), and sheep (Moenter et al., 1993). In rats, Fos protein is first detectable in GnRH neurons after the onset of the expected LH surge, and the number of GnRH neurons expressing Fos increases during the ascending phase of the LH surge (Lee et al., 1990a, 1992b). These results agree with those of the present study showing that c-fos gene expression increases in GnRH neurons near the onset of the LH surge. Synaptic blockade with phenobarbital or MK-801 (an NMDA channel blocker) inhibits the LH surge and the induction of Fos in GnRH neurons (Lee et al., 1990a, 1993), suggesting that ...
Well almost, moving addresses anyway. It wasnt that long ago I moved from Disease of the Week to here at Disease Prone to take advantage of an offer from Field of Science. While I have been super happy here, an amazing opportunity has opened up for me to move the blog over to the Scientific Americans new community and so now I can be found at Disease Prone. I know a few others from FoS are moving too and Ill let them say their own goodbyes but for me, I wanted to say a big thank you to FoS, its bloggers, readers and admin for helping me set up and being so supportive when this offer was extended to me ...
We previously demonstrated that APP epigenetically regulates Egr1 expression both in cultured neurons and in vivo. Since Egr1 is an immediate early gene involved in memory formation, we wondered whether other early genes involved in memory were regulated by APP and we studied molecular mechanisms involved. By comparing prefrontal (PF) cortex from wild type (APP+/+) and APP knockout mice (APP-/-), we observed that APP down regulates expression of four immediate early genes, Egr1, c-Fos, Bdnf and Arc. Down regulation of Egr1, c-Fos and Bdnf transcription resulted from a decreased enrichment of acetylated histone H4 on the corresponding gene promoter. Further characterization of H4 acetylation at Egr1 and c-Fos promoters revealed increased acetylation of H4K5 and H4K12 residues in APP-/- mice. Whereas APP affected Egr1 promoter activity by reducing access of the CREB transcription factor, its effect on c-Fos appeared to depend on increased recruitment of HDAC2 histone deacetylase to the gene ...
TY - JOUR. T1 - The influence of age on the treadmill exercise-induced c-Fos expression in the hippocampus of rats. AU - Kim, Sang Ho. AU - Kim, Hong. AU - Kim, Sung Soo. AU - Shin, Mal Soon. AU - Chang, Hyun Kyung. AU - Lee, Taeck Hyun. AU - Jang, Mi Hyeon. AU - Shin, Min Chul. AU - Lee, Hee Hyuk. AU - Kim, Young Pyo. AU - Kim, Chang Ju. PY - 2004/7/1. Y1 - 2004/7/1. N2 - c-Fos has been used as a neuronal activity marker. Here, we examined the influence of age on the treadmill running-induced c-Fos expression in rat hippocampus. Rats of exercise groups were forced to run on treadmill for 30 min once a day for 5 consecutive days. Without exercise, c-Fos expression was highest in 8-week old rats. Treadmill exercise significantly enhanced the c-Fos expression in the hippocampus of rats in all ages. In the CA region, the increase of the c-Fos expression by treadmill exercise was highest in 4-week old rats. In the dentate gyrus, the increase of the c-Fos expression by treadmill exercise was highest ...
TY - JOUR. T1 - Upregulation of Npas4 protein expression by chronic administration of amphetamine in rat nucleus accumbens in vivo. AU - Guo, Ming Lei. AU - Xue, Bing. AU - Jin, Dao Zhong. AU - Liu, Zhen Guo. AU - Fibuch, Eugene E.. AU - Mao, Li Min. AU - Wang, John Q.. PY - 2012/10/24. Y1 - 2012/10/24. N2 - The neuronal PAS domain protein 4 (Npas4) is a transcription factor that is almost exclusively expressed in the mammalian brain. As an activity-dependent transcription factor, Npas4 regulates the transcription of discrete genes and transcriptionally controls the experience-dependent learning and memory. In this study, we explored the impact of the psychostimulant amphetamine (AMPH) on Npas4 protein expression in the rat striatum. We found that acute systemic injection of AMPH had a minimal effect on protein levels of Npas4 in the caudate putamen (CPu) and nucleus accumbens (NAc), while AMPH readily increased protein products of the immediate early gene c-Fos in these regions. In contrast, ...
Effect of administration of high dose intrathecal clonidine or morphine prior to sciatic nerve section on c-Fos expression in rat lumbar spinal cord.: The effec
The goal of this study is to identify reward-related distributed brain networks by delineating a reliable immunohistological technique...
The inhibition of GLUT2-mediated sugar detection increased food intake in GLUT2-SDD mice (line TgG) by enlarging meal sizes without changes in meal frequency. The decision to stop eating is delayed in conjunction with defects in arcuate c-Fos activation in response to glucose and changes in orexin and TRH neuropeptide mRNA levels in the hypothalamus of fed mice. Thus, GLUT2 receptor function, independent of sugar transport and metabolism, is involved in controlling feeding behavior.. GLUT2 plays a key role in glucodetection, which controls the feeding behavior in mice. Indeed, daily food intake is similarly increased in GLUT2-SDD (line TgG) and in GLUT2-null rescued (ripglut1;glut2−/−) mice (5), and in both mouse models, provision of glucose failed to reduce food intake. GLUT2-dependent sugar transport (except in pancreatic β-cells) and sugar detection were invalidated in GLUT2-null mice; by contrast, GLUT2-SDD mice lack only the receptor function, and thus sugar transport was still able to ...
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SEATTLE, March 12, 2013 /PRNewswire/ -- Seattle/Tacoma CFOs Report Second-Quarter Hiring Plans. One in 5 Companies Plan to Create Professional-Level Jobs...
This thesis examines mechanisms of regulation of neuropeptide gene expression in vivo in some neurosecretory hypothalamic neurones of the rat. In particular, the influence of neural pathways, acting via receptors and subsequent regulation of genetic transcription factors was measured and second messenger pathways were directly manipulated and the effects of their mutation on gene expression were measured. The magnocellular neurones of the supraoptic nucleus (SON) are known to be directly (i.e. non-synaptically) osmosensitive. Fos, the protein product of the immediate early gene c-fos, has been used as a marker of neuronal activation and its expression is induced in these neurones by increasing systemic hyperosmolarity. The effects of acute, direct hyperosmotic stimulation, via a microdialysis probe, on Fos expression in supraoptic nucleus magnocellular neurones was investigated. Fos expression was detected by immunohistochemistry (IHC). However, direct hyperosmotic stimulation failed to induce ...
Immobilization stress upregulates c-Fos expression in several CNS areas. Repeated stress or the use of drugs can modulate stress-induced c-Fos expression. Here, we investigated in 40 different areas of the rat brain the effects of dexamethasone (SDX, a synthetic glucocorticoid), diazepam (SBDZ, a benzodiazepine), and imipramine (IMI, an antidepressant) on the c-Fos expression induced by restraint stress. Wistar rats were divided into four groups and submitted to 20 days of daily injection of saline (three first groups) or imipramine, 15 mg/kg, i.p. On day 21, animals were submitted to injections of saline (somatosensory, SS), SDX (1 mg/kg, i.p.), SBDZ (5 mg/kg, i.p.), or IMI (15 mg/kg, i.p.) before being submitted to restraint. Immediately after stress, the animals were perfused and their brains processed with immunohistochemistry for c-Fos (Ab-5 Oncogene Science). Dexamethasone reduced stress- induced c-Fos expression in SS cortex, hippocampus, paraventricular nucleus of the hypothalamus (PVH), ...
Due to the poor correlation between steady state mRNA levels and protein products, traditional microarray analysis may miss many genes which are regulated primarily at the level of mRNA stability and translation. Posttranscriptional gene regulation is becoming increasingly recognized as an important form of cellular control. The importance of microRNAs and RNA binding proteins (RBPs) is now beginning to be better appreciated. We study the elav (embryonic lethal abnormal vision) family of RBPs, which are paraneoplastic antigens, over-expressed in a variety of malignancies, including breast cancer. Antibodies against elav family members are believed to be cancer-protective. The elav family of RBPs binds to the AU-rich elements (AREs) found in the 3\#8217; untranslated regions (UTRs) of many early-response genes, including proto-oncogenes and cell cycle regulators. HuR, the ubiquitously expressed family member, has been described to play a role in cancer progression. HuR stabilizes and ...
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The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Drosophila Fos antibody (kayak) for IHC, IM, WB. Anti-Drosophila Fos pAb (GTX47892) is tested in Drosophila samples. 100% Ab-Assurance.
Define FOS. FOS synonyms, FOS pronunciation, FOS translation, English dictionary definition of FOS. abbreviation for fructooligosaccharide: a chain polymer of fructose found in a variety of plants, vegetables, and fruits and used as a sweetener
TY - JOUR. T1 - Function of Fos proteins in bone cell differentiation. AU - Matsuo, K.. AU - Jochum, W.. AU - Owens, J. M.. AU - Chambers, T. J.. AU - Wagner, E. F.. PY - 1999/7/1. Y1 - 1999/7/1. UR - http://www.scopus.com/inward/record.url?scp=0344867028&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0344867028&partnerID=8YFLogxK. U2 - 10.1016/S8756-3282(99)00120-9. DO - 10.1016/S8756-3282(99)00120-9. M3 - Article. C2 - 10423040. AN - SCOPUS:0344867028. VL - 25. JO - Bone. JF - Bone. SN - 8756-3282. IS - 1. ER - ...
Spoštovani!. Junijsko srečanje Sekcije za klinično nevrofiziologijo bo v petek, 17. 6. 2016, ob 12:00 v seminarju v kleti Nevrološke klinike UKC Ljubljana.. Na sporedu bo predavanje:. Jurij Dreo ...
The levels of c-myc, c-fos, and JE mRNAs accumulate in a biphasic pattern following infection of quiescent BALB/c 3T3 mouse cells with polyomavirus. Maximal levels of c-myc and c-fos mRNAs were seen within 1 hr and were nearly undetectable at 6 hr after infection. At 12 hr after infection mRNA levels were again maximal and remained elevated thereafter. Empty virions (capsids) and recombinant VP1 protein, purified from Escherichia coli, induced the early but not the late phase of mRNA accumulation. Virions, capsids, and recombinant VP1 protein stimulated [3H]thymidine nuclear labeling and c-myc mRNA accumulation ina dose-responsive manner paralleling their affinity for the cell receptor for polyoma. The second phase of mRNA accumulation is regulated by the viral early gene products, as shown by polyomavirus early gene mutants and by a transfected cell line (336a) expressing middle tumor antigen upon glucocorticoid addition. These results suggest that polyomavirus interacts with the cell membrane ...
Reduced methylation of DNA may contribute to loss of the normal controls on proto-oncogene expression. Spontaneous contractile activites of regular frequency and moderate intensity viagra tablets were observed in the MTOs of all specimens examined. Identifying the most efficient items from ...
TY - JOUR. T1 - Serotoninergic neurons in the brainstem expressing FOS protein after orofacial noxious stimulation: an immunocytochemical double-labeling study. AU - Lang, B. AU - Li, Y Q. PY - 1998. Y1 - 1998. N2 - Employing an immunocytochemical double-labeling technique, we investigated the co-localization of FOS protein, the expression product of c-fos proto-oncogene induced by orofacial noxious stimulation, and serotonin in the rat brainstem. About 3.2%-25.6% of serotonin-like immunoreactive neurons and 6.2%-46.7% of FOS-like immunoreactive neurons in the raphe nuclei, reticular formation and ventrolateral subdivision of the midbrain periaqueductal gray exhibit FOS-like immunoreactivity and serotonin-like immunoreactivity, respectively. The present results provide further morphological evidence for the involvement of serotoninergic neurons in modulating the transmission of noxious information.. AB - Employing an immunocytochemical double-labeling technique, we investigated the ...
Home , Papers , Evaluation of c-Fos immunoreactivity in the rat brainstem nuclei relevant in migraine pathogenesis after electrical stimulation of the trigeminal ganglion. ...
Fos induction in the rostral ARC following male exposure.Representative bright-field photomicrographs showing greater Fos immuno-localization in anestrous ewes
The function of striatopallidal neurons is regulated by N-methyl-d-aspartate (NMDA) and dopamine D2 receptors. Previous studies show that immediate early gene induction by D2 receptor blockade is suppressed by NMDA receptor antagonists. Because the pharmacology of NMDA receptors depends on the incorporation of different NR2 subunits and NR2 subunits show regional and cellular differences in their expression in striatum, our study examined whether different NMDA receptor antagonists would have differential effects on eticlopride-induced immediate early gene expression in striatum. Male Sprague-Dawley rats were pretreated with vehicle, CGS 19755, MK-801 or ifenprodil. Rats then received injections of eticlopride and were killed 40 min later. In situ hybridization histochemistry was used to determine the expression of c-fos andzif268 in the striatum. Eticlopride increased immediate early gene expression in striatum, with the increase generally being greater in lateral than in medial striatum. ...
TY - JOUR. T1 - Interleukin-4 regulates macrophage interleukin-12 protein synthesis through a c-fos mediated mechanism. AU - Roy, Sabita. AU - Charboneau, Richard. AU - Melnyk, Dean. AU - Barke, Roderick A.. PY - 2000/1/1. Y1 - 2000/1/1. N2 - Background. Interleukin-4 (IL-4) treatment after lipopolysaccharide (LPS) induction inhibits macrophage (Mφ) IL-12 synthesis; however, IL-4 pretreatment (PreTx) primes the Mφ for increased LPS-induced IL-12 production. In this study we study the role of c-fos in the IL-4 priming of Mφ IL-12 synthesis. Methods. With a murine in vitro peritoneal Mφ model, we studied the effect of either c-fos deficiency (wild type, WT; homozygous c-fos knockout, Homo KO) or c-fos overexpression to study the role of c-fos in IL-4 priming of LPS-induced Mφ IL-12 synthesis. Results. (1) We first show that IL-4 PreTx results in a 72% decrease in Mφ c-fos mRNA compared with vehicle PreTx. (2) With respect to IL-12 p70 protein, IL-4 PreTx in the WT group increased LPS-induced ...
In the analyses of FLI, macronutrient infusions as a whole had their greatest effect on the region of the NTS immediately subjacent to the AP, with fewer cells expressing Fos in the NTS rostral to the AP and in the AP itself and even fewer cells showing Fos in the NTS caudal to the AP. This result was not surprising, because the NTS is larger and contains more neurons in the region of the AP than in its more rostral or caudal regions. Duodenal nutrient infusions had no detectable effect on Fos in the dmnX. These results agree with work by Zittel et al. (33), where lipid and glucose infusions induced greater Fos labeling in the more rostral NTS (subjacent to the AP and the 4th ventricle) than in the caudal NTS (at obex). In an earlier study by Olson et al. (13), Fos expression was determined after treatments that inhibited food intake (e.g., CCK, food ingestion) or potentiated food intake (deprivation or insulin). Although the rostrocaudal distribution of Fos expression was not described, ...
TransAM AP-1 Kits are DNA-binding ELISAs that quantify activated c-Fos, FosB, c-Jun, JunB, JunD & Fra-1 transcription factors using a method that is faster and more sensitive than gelshift, without radioactivity and gels.
WASHINGTON, DC - December 9, 2014 - More than half of corporate treasurers and CFOs believe business conditions will improve in 2015, the largest percentage predicting improvement since 2005, according to a survey of 856 executives released today by the Association for Financial Professionals (AFP), a global professional society.. The AFP Business Outlook Survey, which has tracked business predictions of CFOs, corporate treasurers and other financial executives for the last 11 years, found that 44 percent of finance executives expect the U.S. economy to grow between 2.0 and 2.9 percent, while 31 percent are even more optimistic and expect it to grow between 3.0 and 3.9 percent.. Finance executives expect growth to occur in the second half of 2015, creating another 1.9 million nonfarm jobs. Among those surveyed, 49 percent say their companies anticipate hiring workers in 2015.. EXPECTATIONS FOR 2015:. ...
Isabelle Dunand-Sauthier, Marie-Laure Santiago-Raber, Leonardo Capponi, Charles E. Vejnar, Olivier Schaad, Magali Irla, Queralt Seguin-Estévez, Patrick Descombes, Evgeny M. Zdobnov, Hans Acha-Orbea and Walter Reith ...
Theres no question that CFOs are increasing spending on cybersecurity. While many are doing so in an effort to protect against threats, others also see cybersecurity investment as an opportunity to innovate.. ...
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