View Notes - Biology 1201 from BIOL 1201 at LSU. Eucaryotic Gene Regulation Genome Structure, Genes, DNA, and chromosomes Complete Genome DNA sequence know humans ,chimps ,flies ,worms, and plants…
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Using the dominant control region (DCR) sequences that flank the beta-globin gene locus, we have been able to achieve high-level expression of the human alpha-globin gene in transgenic mice. Expression in fetal liver and blood is copy number dependent and at levels comparable to that of the
Mutators can succeed: We have demonstrated that in continually changing environments where the adaptation to any given environmental state is independent of the adaptation to other states, mutator genes can climb to prominence by associated selection (i.e., by hitchhiking) with the linked beneficial mutations they generate. We have shown that even when mutators are extremely rare, there is a substantial probability that they will increase in frequency. We see this nonequilibrium analysis as a reminder that the average behavior of a process is sometimes an inadequate descriptor of the system as a whole. Although mutators are often lost in our model, in reality there would be recurrent mutation to the mutator state, and even if they are occasionally lost, organisms carrying a mutator gene would eventually dominate. Eventually, however, could be a very long time so that we should be unsurprised to sometimes observe low mutator frequencies even in systems that do conform to the assumptions of our ...
The methanol dehydrogenase gene mxaF, encoding the large subunit of the enzyme, was amplified from the DNA of a number of representative methanotrophs, methyletrophs, and environmental samples by PCR using primers designed from regions of conserved amino acid sequence identified by comparison of three known sequences of the large subunit of methanol dehydrogenase. The resulting 550-bp PCR products were cloned and sequenced. Analysis of the predicted amino acid sequences corresponding to these mxaF genes revealed strong sequence conservation. Of the 172 amino acid residues, 47% were conserved among all 22 sequences obtained in this study. Phylogenetic analysis of these MxaF sequences showed that those from type I and type II methanotrophs form two distinct clusters and are separate from MxaF sequences of other gram-negative methylotrophs. MxaF sequences retrieved by PCR from DNA isolated from a blanket bog peat core sample formed a distinct phylogenetic cluster within the MxaF sequences of type ...
Tetracycline-responsive transcriptional activator driven by the liver-specific mouse major urinary protein promoter (MUP-tTA).. The E. Coli tetracycline operon regulatory system was used to generate a liver-specific transcription activation system that was inhibited by tetracycline. The transcription activator was a fused protein consisting of a tetracycline repressor gene (tetR) that was only active in the presence of tetracycline and a herpes simplex virus protein (VP-16) transcription activating domain (Tet-Off). Transcription was induced only in the absence of tetracycline (Tet-Off). A liver-specific promoter such as the mouse major urinary protein (MUP) promoter determined that the tetracycline-regulated transcriptional activator (tTA) would be expressed specifically in liver. To study the effect of the transcription activator on a target gene (for example, beta-galactosidase, LacZ) specifically in liver, MUP-tTA mice would be mated with transgenic mice in which the TAg Target gene was ...
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Complete information for LOC100420794 gene (Pseudogene), RE1 Silencing Transcription Factor Pseudogene, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ATP5PBP6 gene (Pseudogene), ATP Synthase Peripheral Stalk-Membrane Subunit B Pseudogene 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Publisher: PLOS (Public Library of Science). Date Issued: 2015-08-10. Abstract: BACKGROUND Automatically detecting gene/protein names in the literature and connecting them to databases records, also known as gene normalization, provides a means to structure the information buried in free-text literature. Gene normalization is critical for improving the coverage of annotation in the databases, and is an essential component of many text mining systems and database curation pipelines. METHODS In this manuscript, we describe a gene normalization system specifically tailored for plant species, called pGenN (pivot-based Gene Normalization). The system consists of three steps: dictionary-based gene mention detection, species assignment, and intra species normalization. We have developed new heuristics to improve each of these phases. RESULTS We evaluated the performance of pGenN on an in-house expertly annotated corpus consisting of 104 plant relevant abstracts. Our system achieved an F-value of ...
Using the dominant control region (DCR) sequences that flank the beta-globin gene locus, we have been able to achieve high-level expression of the human alpha-globin gene in transgenic mice. Expression in fetal liver and blood is copy number dependent and at levels comparable to that of the endogenous mouse alpha-globin genes. Transgenic fetuses with high-copy numbers of the transgene suffer severe anemia and die before birth. Using a construct with both the human alpha- and beta-globin genes and the beta-globin DCR, live mice with low-copy numbers were obtained. Both human globin genes are expressed at high levels in adult red cells to give human hemoglobin HbA in amounts equal to or greater than endogenous mouse hemoglobin. Expression of HbA in murine red cells is not accompanied by any increase in mean corpuscular volume (MCV) or mean corpuscular hemoglobin concentration (MCHC). However, these transgenic mice tend to have an increased number of reticulocytes in peripheral blood; consistent with some
Caronte (es); Caronti (co); Charon (en-gb); Харон (bg); Charon (mythology) (simple); Charón (sk); Харон (uk); 카론 (ko); Karono (eo); Charón (cs); Haron (bs); Caront (an); Charon (fr); Haron (hr); Charon (thần thoại Hy Lạp) (vi); Charon (af); Харон (sr); Caronte (pt-br); Charon (sco); Charon (lb); Karon (nn); Kharon (nb); Charon (en); خارون (ar); Charon (br); Kharón (hu); Karonte (eu); Caront (ca); Charon (de-ch); Charon (cy); Carón (ga); خارون (fa); 卡戎 (zh); Charon (da); ქარონი (ka); カローン (ja); כארון (he); Charon (la); 卡戎 (wuu); Kharon (fi); Χάρων , Charōn (grc); Caronte (it); Charon (et); Haron (sh); Caronti (scn); Caronte (pt); Kharon (id); แครอน (th); Charon (en-ca); Charonas (lt); Haron (sl); Kharoon (tr); Харон (ru); Charon (de); Charon (war); Charon (pl); Charon (oc); Charon (nl); Харон (sr-ec); Charon (ro); Caronte (lij); Charon (nds); Caronte (gl); Charon (sv); Χάρων (el); Քարոն ...
Anthropogenetical Analysis of Abnormal Human -globin Gene Cluster Arrangement on Chromosome 16*. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
το κείμενο με τίτλο GPSy: a cross-species gene prioritization system for ... - HAL σχετίζετε με Βιοτεχνολογία
[Molecular mechanisms of globin gene regulation and disregulation].: Human globin genes are expressed in tissue and developmental stage specific manners. Variou
Previous work has suggested that the promoter regions of the human embryonic zeta 2 and epsilon globin genes contain negative regulatory regions that could play a role in the repression of these genes in postembryonic erythroblasts. We have examined this possibility by studying the expression of these genes in mouse erythroleukemia cells, an adult erythroid cell line that might be expected to contain repressor molecules that would bind to the putative negative regulatory regions. When attached to appropriate upstream regulatory elements (alpha HS-40 and beta HS1,2) both the zeta and epsilon genes were expressed in these cells at a low level, but no increase in expression was observed when similar constructs lacking the proposed negative regulatory sequences were introduced into these cells. These results cast doubt on the possibility that these sequences play a major role in the developmental repression of the embryonic globin genes, unless they function only in a normal chromosomal organization.
Histones are the major protein component of nucleosomes, and de novo histone synthesis is essential for packaging newly replicated DNA into chromatin. As a result, histone gene expression is exquisitely and functionally coupled with DNA replication. Vastly divergent organisms such as yeast, fly and human all demonstrate the phylogenetically conserved propensity to maintain clustering of histone genes at one or more genomic loci. Although specific mechanisms are unclear, clustering is presumed to be important for common stringent transcriptional control of these genes at the G1/S phase transition. In this study, we describe a genomic duplication of the human histone gene cluster located at chromosome 1q21, which effectively doubles the previously known size and gene number of that cluster. The duplication persists in all examined tissues and cell lines, and the duplicated genes are transcriptionally active. Levels of messenger RNAs for duplicated histone H4 genes are high relative to those for non
TY - JOUR. T1 - Hpal polymorphic site 3 of the human β-globin gene is inside a repetitive sequence and cannot be ascertained by polymerase chain reaction. AU - Wang, X.. AU - Bouhassira, Eric E.. PY - 1992. Y1 - 1992. KW - Haplotype. KW - Kpnl sequence. KW - PCR. UR - http://www.scopus.com/inward/record.url?scp=0026603960&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0026603960&partnerID=8YFLogxK. M3 - Article. VL - 39. SP - 226. EP - 227. JO - American Journal of Hematology. JF - American Journal of Hematology. SN - 0361-8609. IS - 3. ER - ...
A 2.4 kb region of theEuglena gracilis chloroplast genome containing the genespsbT, psbH andpsbN was characterized. The mRNAs transcribed frompsbB, psbT, p
Exon sequences are conserved, but intron sequences vary, Organization of Genetic Material Split Genes, Overlapping Genes and Pseudogenes, Genetics
Cosmid clones containing alpha 1-antitrypsin (alpha 1AT) gene sequences were observed to contain alpha 1AT-like sequences approximately 12 kb downstream of the authentic alpha 1AT gene. Restriction mapping suggested the alpha 1AT-like gene lacks promoter sequences. Cosmid clones from one library con …
0001.scaffold00002 AUGUSTUS gene 1386 2772 0.12 + . ID=Bv_00001z1_qhas;Name=Bv_00001z1_qhas 0001.scaffold00002 AUGUSTUS mRNA 1386 2772 0.12 + . ID=Bv_00001z1_qhas.t1;Parent=Bv_00001z1_qhas;Name=Bv_00001z1_qhas.t1 0%;Note=cDNAcoverage_0% 0001.scaffold00002 AUGUSTUS five_prime_UTR 1386 1976 . + . ID=Bv_00001z1_qhas.t1.UTR;Parent=Bv_00001z1_qhas.t1 0001.scaffold00002 AUGUSTUS start_codon 1977 1979 . + 0 ID=Bv_00001z1_qhas.t1.start_codon;Parent=Bv_00001z1_qhas.t1 0001.scaffold00002 AUGUSTUS CDS 1977 2325 0.96 + 0 ID=Bv_00001z1_qhas.t1.CDS;Parent=Bv_00001z1_qhas.t1 0001.scaffold00002 AUGUSTUS intron 2326 2619 0.81 + . ID=Bv_00001z1_qhas.t1.intron;Parent=Bv_00001z1_qhas.t1 0001.scaffold00002 AUGUSTUS CDS 2620 2747 0.8 + 2 ID=Bv_00001z1_qhas.t1.CDS;Parent=Bv_00001z1_qhas.t1 0001.scaffold00002 AUGUSTUS stop_codon 2745 2747 . + 0 ID=Bv_00001z1_qhas.t1.stop_codon;Parent=Bv_00001z1_qhas.t1 0001.scaffold00002 AUGUSTUS three_prime_UTR 2748 2772 . + . ID=Bv_00001z1_qhas.t1.UTR;Parent=Bv_00001z1_qhas. ...
Each gene with an approved VGNC symbol has its own Symbol Report that contains our manually curated data and links to other external biomedical resources. The VGNC core data is displayed at the top of the page in a separate box and presents the approved nomenclature, the unique VGNC ID number, aliases, previous nomenclature, locus type, chromosomal location and gene group. The table below the VGNC core data provides links to external resources such as homologs in other species, gene resources, protein resources, and publications. ...
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
A 350-bp segment of DNA associated with an erythroid-specific DNase I-hypersensitive site (HS-40), upstream of the alpha-globin gene cluster, has been identified as the major tissue-specific regulator of the alpha-globin genes. However, this element does not direct copy number-dependent or developmentally stable expression of the human genes in transgenic mice. To determine whether additional upstream hypersensitive sites could provide more complete regulation of alpha gene expression we have studied 17 lines of transgenic mice bearing various DNA fragments containing HSs -33, -10, -8, and -4, in addition to HS -40. Position-independent, high-level expression of the human zeta- and alpha-globin genes was consistently observed in embryonic erythroid cells. However, the additional HSs did not confer copy-number dependence, alter the level of expression, or prevent the variable down-regulation of expression in adults. These results suggest that the region upstream of the human alpha-globin genes is not
The International System for Human Gene Nomenclature (ISGN) was inaugurated in 1979, and has been continually updated. The Human Gene Mapping Nomenclature Committee, which developed the ISGN, put forth a
The International System for Human Gene Nomenclature (ISGN) was inaugurated in 1979, and has been continually updated. The Human Gene Mapping Nomenclature Committee, which developed the ISGN, put forth a
Rat Genomic DNA High molecular weight, high purity DNA for any application - Find MSDS or SDS, a COA, data sheets and more information.
You searched for: Journal Theoretical and applied genetics Remove constraint Journal: Theoretical and applied genetics Source 1990 v.80 no.3 Remove constraint Source: 1990 v.80 no.3 Subject DNA Remove constraint Subject: DNA Subject restriction mapping Remove constraint Subject: restriction mapping ...
Consider two unlinked genes P and Q A plant with genotype PPQQ was crossed with another plant with genotype ppqq to obtain F1 progeny If the F1 progeny to testcrossed the percentage of resultant plants
inproceedings{8564264, author = {{Mus, Liselot and Denecker, Geertrui and Zeltner, Nadja and Ogando, Yudelca and Van Haver, Stéphane and Sanders, Ellen and Jacobs, Eva and Popovic, Mina and Van Neste, Christophe and Vanhauwaert, Suzanne and Durinck, Kaat and Menten, Björn and De Preter, Katleen and Heindryckx, Björn and Studer, Lorenz and Roberts, Stephen and Speleman, Franki}}, booktitle = {{Advances in Neuroblastoma Research, Congress abstracts}}, language = {{eng}}, location = {{San Fransisco, CA, USA}}, title = {{Exploring the contribution of gene dosage effects of 17q gain on ESC and neuroblastoma proliferation}}, year = {{2018 ...
Distribution of the mRNA/peptide in the cardiovascular system Southern blot analysis of human genomic DNA under low hybridization stringency with a 42-mer synthetic oligonucleotide probe corresponding to amino acid residues 7-20 of ET, showed that three different restriction fragments were always detected regardless of the restriction endonucleases used. The nucleotide sequences encoding amino acid residues of the three ETs are highly conserved among the three genes, with 77-82% of the nucleotide residues being identical [2]. By contrast, the nucleotide sequences upstream from the mature peptides are very poorly conserved. These observations suggest that although the three genes are evolutionally relatively distant from each other, the genes evolved from a common ancestral gene under strong pressure to preserve mature ET sequences. The three peptides were designated ET-1, ET-2 and ET-3 [5]. ET-1 is the original peptide corresponding to that detected in the culture medium of porcine aortic ...
We previously reported the isolation and characterization of a cDNA clone, I-309, that encodes a small secreted protein produced by activated human T lymphocytes. This protein is structurally related to a large number of recently identified proteins that are secreted upon cellular activation. In this report we describe the isolation and characterization of the gene encoding I-309. The genomic organization is essentially identical to that found in the genes encoding the structurally similar proteins TCA-3, hJE/MCP-1, and mJE, strengthening the hypothesis that these genes are evolutionarily related. The region of the I-309 gene 5 of the mRNA cap site exhibits extensive nucleotide sequence homology with the same region of the murine gene TCA-3, providing additional evidence that I-309 and TCA-3 are likely to be homologs. Finally, panels of rodent-human somatic cell hybrids were used to map the I-309 gene to human chromosome 17. In conjunction with recent mapping data from other laboratories, this ...
A multiple catheter assembly (100) including a first catheter (110) constructed from a first material and having a first proximal end region (112), a first distal end region (114) terminating in a first distal tip (116), and an outer surface (120) defining at least a first lumen (122) extending longitudinally therethrough between a first distal (118) and a first proximal (111) opening. The first proximal end region (112) is integrally connected to an extension tube (320) constructed from a second material. A second catheter (130) is constructed from the first material and having a second proximal end region (132), a second distal end region (134) terminating in a second distal tip (136), and a second outer surface (140) defining at least a second lumen (142) extending longitudinally therethrough between a second distal (138) and a second proximal (131) opening. The second proximal end region (132) is integrally connected to an extension tube (350) constructed from the second material. The first lumen
We have cloned and characterized the Na,K-ATPase β3 subunit gene (ATP1B3), and a β3 subunit pseudogene (ATP1B3P1), from a human PAC genomic library. The β3 subunit gene is > 50 kb in size and is split
Bordelon, M R., Malignant characteristics of somatic cell hybrids of normal human and malignant mouse cells. Abstr. (1974). Subject Strain Bibliography 1974. 446 ...
Eiglmeier, K., W. Boos, S.T. Cole 1987. Nucleotide sequence and transcriptional startpoint of the glpT gene of Escherichia coli: extensive sequence homology of the glycerol-3-phos transport protein with components of the hexose-6-phos transport system ...
The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...