Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limited success in producing transgenic animals of larger or higher species. Here, we report a linker based sperm-mediated gene transfer method (LB-SMGT) that greatly improves the production efficiency of large transgenic animals. The linker protein, a monoclonal antibody (mAb C), is reactive to a surface antigen on sperm of all tested species including pig, mouse, chicken, cow, goat, sheep, and human. mAb C is a basic protein that binds to DNA through ionic interaction allowing exogenous DNA to be linked specifically to sperm. After fertilization of the egg, the DNA is shown to be successfully integrated into the genome of viable pig and mouse offspring with germ-line transfer to the F1 generation at a highly efficient rate: 37.5% of pigs and 33% of mice. The integration is demonstrated again by FISH
Development of an safe and efficient in vivo gene delivery method is indispensable for molecular biology research and the progress in the following gene therapy. Over the past few years, hydrodynamic gene delivery with naked DNA has drawn increasing interest in both research and potential clinic applications due to its high efficiency and low risk in triggering immune responses and carcinogenesis in comparison to viral vectors. This method, involving intravenous injection (i.v.) of massive DNA in a short duration, gives a transient but high in vivo gene expression especially in the liver of small animals. In addition to DNA, it has also been shown to deliver other substance such as RNA, proteins, synthetic small compounds and even viruses in vivo. Given its ability to robustly mimic in vivo hepatitis B virus (HBV) production in liver, hydrodynamic gene delivery has become a fundamental and important technology on HBV studies in our group and many other groups. Recently, there have been interesting
Novel therapeutic approach using nucleic acid drug and gene delivery method have been developed for the treatment of cardiovascular disease including familial hypercholesterolemia and pulmonary arterial hypertension. In order to develop the novel non-viral method for gene delivery, polyion complex nanomicelles have been used under the collaboration with Professor Kazunori Kataoka (Univ. of Tokyo). We have reported the therapeutic effect of intratracheal administration of adrenomedullin by using PEG-PAsp(DET) in model animals of pulmonary arterial hypertension. This method for gene delivery was shown to be safe compared with that using viral vector. Our gene delivery method can be applied to the treatment of other diseases. ...
Click to launch & play an online audio visual presentation by Prof. Luigi Naldini on Gene transfer strategies: principles, state-of-the-art and the major barriers that need to be overcome 2, part of a collection of online lectures.
Neutralizing antibodies (NAbs) against adeno-associated viruses (AAVs) are known to interfere with AAV vector-mediated gene transfer by intravascular delivery. Consequently, gene transfer studies in non-\human being primates may well forecast the effectiveness of gene transfer in humans. Indeed, gene transfer studies using a fresh type of vector have been carried out in rhesus macaques.12,13 The effects from these studies provided the basis for recent hemophilia B gene therapy clinical tests employing an AAV8 vector.13,14,15,16 Gene transfer in mice using AAV vectors results in excellent transduction efficiency. That is so for AAV8 vector-mediated gene transfer in the mouse liver especially;12,13,14,17 however, the efficiency of AAV8 vectors is modest in macaques.13 Theres also difficulties connected Mouse monoclonal to STYK1 with gene appearance when working with AAV8 vectors in non-human primates. Growing proof suggests that the current presence of neutralizing antibodies (NAbs) against AAV8, ...
Agrobacterium-mediated transformation and direct gene transfer using the gene gun (microparticle -bombardment) are the two most widely used methods for plant genetic modification. The Agrobacterium method has been successfully practiced in dicots for
Gene transfer techniques are an important tool in studies of gene function as well as in the clinical evaluation of new treatments. In research the most important impact of efficient transient and stable gene transfer methods is the generation of new cell lines or animal models for the basic research of protein functions. Many of these methods are based on utilization of viruses as means to target and deliver genes into appropriate cells. Alternatively, recent advances in the RNAi-methodology enable the same delivery method to be used to efficiently silence specific genes in cells.. Successful work with sophisticated viral methods requires special expertise and strict safety considerations both of which are found at all of the biocenters in Finland.. ...
Direct Gene Transfer with IP-10 Mutant Ameliorates Mouse CVB3-Induced Myocarditis by Blunting Th1 Immune Responses. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
TY - JOUR. T1 - Cationic lipid-based gene delivery systems. T2 - Pharmaceutical perspectives. AU - Mahato, Ram I.. AU - Rolland, Alain. AU - Tomlinson, Eric. PY - 1997. Y1 - 1997. N2 - Gene delivery systems are designed to control the location of administered therapeutic genes within a patients body. Successful in vivo gene transfer may require (i) the condensation of plasmid and its protection from nuclease degradation, (ii) cellular interaction and internalization of condensed plasmid, (iii) escape of plasmid from endosomes (if endocytosis is involved), and (iv) plasmid entry into cell nuclei. Expression plasmids encoding a therapeutic protein can be, for instance, complexed with cationic liposomes or micelles in order to achieve effective in vivo gene transfer. A thorough knowledge of pharmaceutics and drug delivery, bio-engineering, as well as cell and molecular biology is required to design optimal systems for gene therapy. This mini-review provides a critical discussion on cationic ...
View Notes - Lecture 9 from PLB PLB113 at UC Davis. PLB 113 Lecture 9 III. Gene Transfer and Epigenetic C. Development of Gene Transfer System D. Transgene Expression E. Cosuppression and Epigenetics
Author: Marichal-Gallardo, Pavel et al.; Genre: Meeting Abstract; Published in Print: 2019; Title: A single-use chromatographic purification platform for viral gene transfer vectors & viral vaccines
The File Transfer Protocol July 8, 1972 the specified data socket, and it is the servers responsibility to initiate the data connection and data transfer in accordance with the specified data connection parameters. It should be noted that the data socket need not be in the same HOST that initiates the FTP commands via the TELNET connection, but the user or his user-FTP process must ensure a "listen" on the specified data socket. A practical example of such file transfer to third HOSTs is a maxi-HOST user (who may actually be a TIP user) whishing to transmit a file to or from an I/O device attached to a TIP. It should also be noted that two data connections, one for send and the other for receive, may exist simultaneously. The protocol requires that the TELNET connections be open while data transfer is in progress. It is the responsibility of the user to close the TELNET connections when finished using the FTP service. The server may abort data transfer if the TELNET connections are closed. III. ...
Cancer is one leading causes of death worldwide. Gene therapy belongs to the promising options for treatment of malignant tumors. The non-viral gene therapy is known as safer alternative to the viral gene therapy. For non-viral gene transfer the vector and the transfer technology are of crucial importance. As part of this work a clinical trial was performed to assess efficiency and safety of the non-viral jet-injection. It was shown, that this technology can be used safely in a clinical setting. As a result of this clinical trial we concluded, that vector safety and especially efficiency need further improvements. Based on this optimized non-viral vectors (minicircle, MIDGE) were compared with each other and their respective parental plasmids. The MIDGE vector showed the highest transgene expression due to increased transcription. In preparation of a clinical trial the combined treatment of hTNF-alpha gene transfer and Vindesine chemotherapy was analyzed. Again, the MIDGE vector showed the ...
Several years ago it was reported that the entry of adenovirus particles could augment the uptake of polylysine-condensed DNA molecules ( Curielet al. 1991), a phenomenon similar to previously...
Image Caption: Illustrates the FTP connection between a Client and a Server, using the PASV command. Credit: Michael Lorer/Wikipedia (CC BY-SA 3.0). File transfer protocol (FTP) is the protocol of transferring files from one host to another host usually over the Internet. This protocol is normally done by authentication by the user entering a username and password. However, some servers (hosts) allow anonymous connection, which in most cases allows limited access to the site.. The first FTP applications were called command-line applications that were developed before computers used graphical user interfaces (GUIs). The later FTP applications shipped with most operating systems still use the command-line concept but is integrated other applications on the PC.. The original FTP was published on April 16 1971, and replaced in June 1980. The current protocol was released in October 1985. Updates to the current FTP were done in June 1997 with security extensions, and in September 1998 with IPv6 ...
To date efficient widespread expression in skeletal and cardiac muscles has not been demonstrated in large animal models of DMD. Intravascular delivery of AAV vectors demonstrated the proof of concept in mdx mice by restoring functional correction of skeletal muscle, diaphragm and heart disease (Bostick et al. 2009; Gregorevic et al. 2006; Wang et al. 2005). In normal neonate dogs, injection of AAV-9 resulted in expression of a reporter gene, in a dose-dependent manner, in widespread skeletal muscle but not in the heart (Yue et al. 2008). In another study, AAV-6 was the most efficient vector serotype in cardiac gene transfer following percutaneous transendo-cardial delivery to normal adult dogs (Bish et al. 2008). In both studies, no cellular immune responses were observed. More studies are required to determine whether these results could address safety scale-up issues in DMD dogs using a relevant therapeutic transgene, and whether the potential risk of immune responses to the vector and/or ...
There is an ongoing, intensive search for novel therapies to improve the prognosis of patients with the most common and aggressive form of primary brain cancer, glioblastoma multiforme (GBM). Gene transfer is one such approach. Early gene-transfer studies with replication incompetent vectors showed this approach to be generally safe, but ineffective due to limited transduction of the tumor. More recently gene transfer has been attempted with oncolytic, replicating viruses. However these viruses are rapidly cleared by the immune system. To overcome these shortcomings of previous gene transfer protocols, Toca 511 has been developed utilizing a Retroviral Replicating Vector (RRV). This platform has the following potential advantages: 1) The vector only infects dividing cells, 2) The virus stably integrates into the genome of the tumor cells allowing for the potential for long-term control of the tumor, 3) The virus is not intrinsically oncolytic and is not cleared from the tumor by the immune ...
Cardiac gene transfer represents a unique strategy to alter the expression of specific genes in the heart that may result in alterations in contractile function. This approach allows us to investigate the role of these pathways on cardiac disease pathogenesis and progression. In rodents, cardiac gene transfer has been achieved predominantly by direct injection into the myocardium or perfusion of an isolated coronary segment. Either approach results in focal overexpression of the transgene and is therefore unlikely to effectively modulate global cardiac function. Here, we provide a gene transfer method that results in global transduction of the heart. We used this technique to demonstrate in vivo that beneficial effects of SUMO1 in the setting of heart failure are dependent on SERCA2a.. ...
Health, ...The challenge of treating patients with genetic disorders in which a s...A group of untreatable muscle disorders known as dysferlinopathies are...Gene therapy using adeno-associated virus (AAV) to deliver genes to ce... We have had success in the clinic using AAV gene therapy with limb gi...,New,gene,transfer,strategy,shows,promise,for,limb,girdle,and,other,muscular,dystrophies,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Progress in gene transfer technology has established the scientific basis for molecular approaches to human diseases. In the kidney, the first trial of gene transfer was reported in 1991, and several...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Human gene therapy is another medical application that is gaining wide acclaim. In essence, it is the transfer of genetic information into patient tissues and organs. As a result, diseased defective copies of genes can be eliminated or their normal functions rescued. Moreover, the procedure can provide new functions to cells. For Example, to combat cancer and other diseases, the insertion of a gene that causes the production of immune system mediator proteins can be introduced. By this therapy, countless genetic disease could have potential cures further down the road. There are two paths to Gene therapy. The first path is direct transfer of genes into the patient. The second path is the use of living cells as vehicles to transport the genes of interest. These two paths both have certain advantages and disadvantages. Direct gene transfer is the most simplistic way of administering the gene of choice. There are two methods to direct gene transfer. The first method is the process in which genes ...
RNAi technology has another key advantage. Scientists need very small doses of siRNA to carry out their experiments; only a small number of the molecules need to enter a cell to exert their silencing effect. On the other hand, says Hagstrom, "the real bottleneck is that you have to get siRNA into cells." Thats not particularly difficult for cells in vitro. But transfecting siRNA into cells in vivo presents a more serious challenge. Mirus, a company that specializes in gene transfer technologies, has developed TransIT-TKO to carry out the task in vitro. "Our formulation is a combination of a polymer and an amphipathic polyamine," says Hagstrom. "were very far along with the technology for in vitro transfection. For in vivo transfection we have had one of the first publications showing highly effective nonviral gene delivery to a mouse liver. In this procedure the nucleic acid is delivered into the bloodstream in a manner that facilitates its delivery to cells outside the blood vessel wall. The ...
Knowledge about the uptake mechanism and subsequent intracellular routing of non-viral gene delivery systems is important for the development of more efficient carriers. In this study we compared two
e. Transformation frequency (% of randomly recovered colonies). In the second procedure, protoplasts were plated immediately after PEG treatment in a 2ml slab of 0.6% low melting agarose (Paszty and Lurquin, BioTechniques 5:716, 1987) in growth medium with 6% mannitol and 25% CM which was then overlaid with less than 0.5ml liquid growth medium also containing 6% mannitol and 25% CM. After 2 weeks, the slabs were sectioned and placed as above on solid nonselective growth medium for additional proliferation of cells for 7d before selection. Rapid growth of discrete microclusters was observed during this second round of growth on nonselective agar. These microclusters are called "colonies" since the original aggregates of PEG- treated protoplasts were maintained as unique entities embedded in agarose. This plating method does not allow further division into sib aggregates during growth on nonselective medium. Further, it is also possible that each original cluster of PEG-treated cells may have ...
Principal Investigator:UMEHARA Takashi, Project Period (FY):1999 - 2000, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Orthopaedic surgery
Sigma-Aldrich offers abstracts and full-text articles by [Ling Li, Fangfang Zhao, Baojing Zhao, Jin Zhang, Chao Li, Renzhong Qiao].
We have developed a proprietary gene therapy platform, the Skin TARgeted Delivery platform, or STAR-D platform, that consists of a patent pending engineered viral vector and skin-optimized gene transfer technology, to develop off-the-shelf treatments for dermatological diseases for which we believe there are no known effective treatments.. Our modified HSV-1 is a replication-deficient, non-integrating viral vector that can efficiently penetrate a broad range of skin cells. Its high payload capacity to accommodate large or multiple genes and low immunogenicity makes it a suitable choice for direct and repeat delivery to the skin.. ...
In vivo gene delivery with non-viral carriers. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Animal homologs of human genetic diseases are used as test systems for gene transfer by viral vectors. The approaches for transferring genes to the brain currently being investigated are ex vivo gene transfer using retrovirus and lentivius vector-modified neural stem cells transplanted to the brain and direct injection of herpesvirus, adeno-associated virus, and lentivirus vectors. The studies involve comparisons of promoters, properties of transduction for different cell types and various subregions of the brain. New methods to follow cell fate and gene expression in the live animal are being explored using MRI and PET techniques. Studies are also being directed towards better understanding of the mechanism of disease in the brain ...
Animal homologs of human genetic diseases are used as test systems for gene transfer by viral vectors. The approaches for transferring genes to the brain currently being investigated are ex vivo gene transfer using retrovirus and lentivius vector-modified neural stem cells transplanted to the brain and direct injection of herpesvirus, adeno-associated virus, and lentivirus vectors. The studies involve comparisons of promoters, properties of transduction for different cell types and various subregions of the brain. New methods to follow cell fate and gene expression in the live animal are being explored using MRI and PET techniques. Studies are also being directed towards better understanding of the mechanism of disease in the brain ...
PDF Gratis herunterladen Gene Therapy for HIV and Chronic Infections (Deutschland) - Ben Berkhout. This book centerson gene therapy and gene transfer approaches to prevent or treat chronic virus...
A command protocol for a single wire bus for transmitting and receiving commands and data. The command protocol includes a serial command word which facilitates communication between a host circuit an
Internet-Draft HTTP/1.1, Part 2 July 2011 PUT successfully creates one, then the origin server MUST inform the user agent by sending a 201 (Created) response. If the target resource does have a current representation and that representation is successfully modified in accordance with the state of the enclosed representation, then either a 200 (OK) or 204 (No Content) response SHOULD be sent to indicate successful completion of the request. Unrecognized header fields SHOULD be ignored (i.e., not saved as part of the resource state). An origin server SHOULD verify that the PUT representation is consistent with any constraints which the server has for the target resource that cannot or will not be changed by the PUT. This is particularly important when the origin server uses internal configuration information related to the URI in order to set the values for representation metadata on GET responses. When a PUT representation is inconsistent with the target resource, the origin server SHOULD either ...
Since the isolation of the GFP from the bioluminescent jellyfish in 1992 and the subsequent development of related molecules, genetically encoded sensors that enable fluorescence imaging of excitable cell activity have been constructed by fusing fluorescent proteins to functional proteins that are involved in physiological signaling. Because these sensors can be introduced by gene transfer techniques, they may extract neuronal signals from an intact brain more efficiently than conventional organic dyes. Also, their expression is driven in a certain population of neurons by the use of a specific promoter; this has made visualization of the connectivity between two or more different (sub)populations of neurons all the more exciting ...
Fuji Xerox developed technology that synthesizes a part of an image into another image or transfers a property of an image to another image to give it a similar look and feel.
Sigma-Aldrich offers abstracts and full-text articles by [Simon N Waddington, John H McVey, David Bhella, Alan L Parker, Kristeen Barker, Hideko Atoda, Rebecca Pink, Suzanne M K Buckley, Jenny A Greig, Laura Denby, Jerome Custers, Takashi Morita, Ivo M B Francischetti, Robson Q Monteiro, Dan H Barouch, Nico van Rooijen, Claudio Napoli, Menzo J E Havenga, Stuart A Nicklin, Andrew H Baker].
The Section on Directed Gene Delivery assesses the ability of viral vectors to efficiently deliver genes to appropriate target cells with the end goal of transf...
Begin Original Message ---- I need to prepare 50mg plasmid DNA. Last year, I prepared 20mg of that = which took me 2 whole months. I really do not want to do it again by myself (= it made me sick). Is there anyone know any commercial company that could do thi= s for me (and better with good price)? Thanks. Wilson (wzhou0 at pop.uky.edu) ---- End Original Message ---- Dear Dr. Wilson, Making loads of endotoxin-free plasmid DNA for direct gene transfer to animals ? ? Try QIAGEN. Heard that they have recently started this new service. They also have a GigaPrep kit for you to do it yourself. The columns look as thick as my forearm. :-) Hope this is useful info. :-) Patrick F.H. Lai , PFHLai at looksmart.com , Graduate Student University of Toronto Toronto, Ontario, Canada LookSmart =85 or keep looking. http://www.looksmart.com ...
Buy Gene Transfer and Expression in Mammalian Cells by S. C. Makrides (9780444513717) from Boomerang Books, Australias Online Independent Bookstore
9780521690843 Our cheapest price for Gene Transfer and the Ethics of First-in-Human Research: Lost in is $13.47. Free shipping on all orders over $35.00.
iRiver has a huge line of flash-based players with various memory sizes (128MB to 2GB). Some of these players may need an updated firmware in order to play Ogg Vorbis files, see the [http://www.iriveramerica.com/support/ support download page] for that. Note - on older players, only certain bitrates are supported, various problems are reported including reboots, silence and random noise when a VBR Vorbis passes outside the limit (either under 96Kbps or over 225 Kbps). Newer players dont have this limitation. However, please be alerted that many of the newer players, such as the Clix, use the Microsoft MTP transfer protocol exclusively so they only work with Windows, whereas other players may be shipped with MTP, but have alternate non-MTP firmware available for download. Tag support not present on U10/Clix (others also?), so Vorbis files will appear under unknown artist/unknown album. Please note that the H10 model does not (yet?) support ogg, and can operate in both MTP and UMS (mass ...
Automated Transfer Vehicle Edoardo Amaldi shipped to its launchpad. The trip takes about one hour as the Automated Transfer Vehicle transporter movesat a speed of 15 kilometres an hour. ESAs third automated space freighter, will carry about two tonnes of dry cargo, 285 kg of water and m…
Quantification of eluted nucleic acids is a critical parameter in characterizing biomaterial based gene-delivery systems. The most commonly used method is to assay samples with an intercalating fluorescent dye such as ...
Its great that we live in an age where any wireless data transfer technology can be considered old school. We still use infrared technology in low-end technology such as TV remotes, but when is the last time you transferred files between two devices using IR? There is no question that Bluetooth ha...
... : Provides high-resolution alkaline transfers of DNA in less than
To facilitate a sale, an increasing number of owners of contaminated property are implementing innovative risk transfer strategies that make it possible to reassign a propertys environmental liabilities to a third party.
I know with my clinic, all patients get 5 day transfers unless the patient specifically asks for a 3 day transfer. It is know the belief that if the embryo doesnt make it to 5 days then it is not chromosomal normal. Do i believe this?. heck who knows. some say its better for the embryo to be in the uterus then in the lab. some clinics better stats with 3 day transfers so that is what they do. Ask your Re why they want to do a day 5. If you want to make sure that you have something to transfer then ask for a day 3 transfer. If you want to have more knowledge on how your embryos grow then wait it out till day 5. For my DE cycle we had 13 eggs, 11 looked awesome on day 3 all grade 1 or 2 and 8+ cells. We let them grow till day 5.. i think 5 stopped growing by day 5. We did CGH testing on the eggs and 7 of the 13 were chromosonally abnormal. So only 2 of the abnormal ones made it to the blast stage. So in my case, i think it does show that it is better to let them grow out till day 5. but it is ...
I know with my clinic, all patients get 5 day transfers unless the patient specifically asks for a 3 day transfer. It is know the belief that if the embryo doesnt make it to 5 days then it is not chromosomal normal. Do i believe this?. heck who knows. some say its better for the embryo to be in the uterus then in the lab. some clinics better stats with 3 day transfers so that is what they do. Ask your Re why they want to do a day 5. If you want to make sure that you have something to transfer then ask for a day 3 transfer. If you want to have more knowledge on how your embryos grow then wait it out till day 5. For my DE cycle we had 13 eggs, 11 looked awesome on day 3 all grade 1 or 2 and 8+ cells. We let them grow till day 5.. i think 5 stopped growing by day 5. We did CGH testing on the eggs and 7 of the 13 were chromosonally abnormal. So only 2 of the abnormal ones made it to the blast stage. So in my case, i think it does show that it is better to let them grow out till day 5. but it is ...
[117 Pages Report] Managed File Transfer Market report categorizes the market by Solutions such as Application-Centric Managed File Transfer, People-Centric Managed File Transfer, and Ad-Hoc Managed File Transfer, by Service, by Deployment and by Geography.