There are some genes that are only activated in the very first days of an embryos existence. Once they have accomplished their task, they are shut down forever, unlike most of our genes, which remain active throughout our ...
Scientists have now discovered a new way to turn off imprinted genes - by chemically tagging histones, proteins that organize DNA.
Buy ARHGAP1 gene silencers from Santa Cruz. RNAi products available in siRNA, shRNA Plasmid and shRNA Lentiviral Particle formats.
C75 The discovery of frequent inactivation of BLIMP1 in activated B cell-like DLBCLindicates that BLIMP1 has an important role in the pathogenesisof this lymphoma subtype, and possibly functions as a tumor suppressor gene. However a recent report shows that the presence of variable percentages of BLIMP1-positive tumor cells in about half of DLBCL cases, and patients with higher BLIMP1 expression are significantly correlated with shorter failure free survival. This raises the possibility that higher BLIMP1 expression could possibly promote the development of lymphoma. Deregulated BCL-6 expression contributes to lymphomagenesis in part by functional inactivation of p53. We found that BLIMP1 acts in an auto-regulatory feedback loop that controls p53 activity through repression of p53 transcription. Our finding that BLIMP1 represses p53 expression suggests that high expression of BLIMP1 might also promote the development of tumor by suppression of p53 activation. It would be of interest to study the ...
Sense Transgene-induced Silencing is Impaired in dcl2 Mutant Plants, but Enhanced in dcl4 Mutants.(A) The diagram shows the coding region of the silenced GUS se
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(Medical Xpress)-A study led by Yale Cancer Center may provide clues to how some aggressive cancers turn off, or silence, genes critical to suppressing tumors. The findings, published in the journal Cell Reports, suggest ...
Implementation of two-region transition matrix T. Silent state is replaced by the begin state in first row and by end state in last column. Each row is norma
Gentaur molecular products has all kinds of products like :search , EpigenTek \ EpiQuik DNA Methyltransferase DNMT Activity_Inhibition Assay Kit Fluorometric \ P-3004-48 for more molecular products just contact us
During meiosis in C. elegans, unpaired chromosomes and chromosomal regions accumulate high levels of histone H3 lysine 9 dimethylation (H3K9me2), a modification associated with facultative heterochromatin assembly and the resulting transcriptional silencing. Meiotic silencing of unpaired DNA may be …
The past decade has witnessed a true revolution in our understanding of how RNA molecules can reduce or silence gene expression, a process that holds great promise in basic research and for antiviral and anticancer applications. In RNA Silencing: Methods and Protocols, many of the most notable and distinguished researchers in the field describe in step-by-step detail their best methods for the design, preparation, and use of RNAs to reduce gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology series format, each offering ...
RNA interference (RNAi) is an important pathway that is used in many different organisms to regulate gene expression. This animation introduces the principles of RNAi involving small interfering RNAs (siRNAs) and microRNAs (miRNAs). We take you on an audio-visual journey through the steps of gene expression and show you an up-to-date view of how RNAi can silence specific mRNAs in the cytoplasm.. ...
One type involves attaching methyl groups to pieces of DNA called promoters, which reside immediately upstream of individual gene sequences (between genes), that tell the machinery that follows genetic instructions to start reading here. The attachment of a methyl group to a promoter generally interferes with this and silences a nearby gene. However, ancient organisms such as plants and insects have less methylation between their genes, and more of it within the coding regions of the genes themselves (within gene bodies). Such gene-body methylation has been shown to encourage rather than silence gene expression ...
Gene silencing through RNA interference (RNAi) has emerged as a potential strategy in manipulating cancer causing genes by complementary base-pairing mechanism. Small interfering RNA (siRNA) is an important RNAi tool that has found significant application in cancer therapy. However due... Read more ...
Cyclin-dependent kinase 9 (CDK9) promotes transcriptional elongation through RNAPII pause release. We now report that CDK9 is also essential for maintaining gene silencing at heterochromatic loci. Through a live cell drug screen with genetic confirmation, we discovered that CDK9 inhibition reactivat …
RNAi is a widely used methodology for gene silencing. The action mechanism of siRNA molecules has been well studied in recent years, and the technique..
Is there such a thing as a dog silencer to stop dog barking? Bark Off claims it has the best dog silencer on the market so read reviews before you buy online or in stores.
Methylation of CpG island promoters is an epigenetic event that can effectively silence transcription over multiple cell generations. was achieved specifically through Tet3-mediated hydroxymethylation. Collectively our findings reveal a new mechanism that may be exploited to facilitate therapeutic DNA demethylation to reverse kidney fibrosis. In recent years epigenetics have emerged as determinants of fibrosis in the kidney (and other tissues as well).1-5 Furthermore epigenetics have been implied to contribute to the individual susceptibilities of CKD patients to develop fibrosis.1-3 Among the known epigenetic mechanisms methylation of CpG island promoters (referred to as DNA methylation) is the most potent to silence transcription of affected genes.6 Because transcriptional silencing of affected genes has been shown to causally contribute to fibroblast activation and Sapitinib fibrogenesis inhibition or reversal of such aberrant methylation is considered beneficial for the kidney.3 Although in ...
CpG island hypermethylation is an epigenetic control aberration that is important for gene inactivation in cancer cells. Hypermethylation of CpG islands has been described in almost every type of tumor. Many important cellular pathways, such as DNA repair (hMLH1, for example), cell cycle (p14ARF), apoptosis (DAPK), cell adherence (CDH1, CDH13), are inactivated by this epigenetic lesion. Hypermethylation is linked to methyl-binding proteins, DNA methyltransferases and histone deacetylase, but the degree to which this process selectively silences tumor suppressor genes continues to remain a vibrant field of study. The list for hypermethylated genes is growing and functional and genetic studies are being performed to determine which hypermethylation events are relevant for tumorigenesis. Basic as well as translational research will be needed to understand the mechanisms and roles of CpG island hypermethylation in cancer. The first discovery of methylation in a CpG island of a tumor suppressor gene ...
TY - JOUR. T1 - R-Loop Formation Is a Distinctive Characteristic of Unmethylated Human CpG Island Promoters. AU - Ginno, Paul A.. AU - Lott, Paul L.. AU - Christensen, Holly C.. AU - Korf, Ian F. AU - Chedin, Frederic. PY - 2012/3/30. Y1 - 2012/3/30. N2 - CpG islands (CGIs) function as promoters for approximately 60% of human genes. Most of these elements remain protected from CpG methylation, a prevalent epigenetic modification associated with transcriptional silencing. Here, we report that methylation-resistant CGI promoters are characterized by significant strand asymmetry in the distribution of guanines and cytosines (GC skew) immediately downstream from their transcription start sites. Using innovative genomics methodologies, we show that transcription through regions of GC skew leads to the formation of long R loop structures. Furthermore, we show that GC skew and R loop formation potential is correlated with and predictive of the unmethylated state of CGIs. Finally, we provide evidence ...
The maintenance methyltransferase Dnmt1 and the de novo methyltransferase Dnmt3b cooperate to maintain DNA methylation and genomic stability in the adult intestinal epithelium.
Fu et al. demonstrate a requirement for the heterochromatin factors CMT and DDM1 in RNA-directed DNA methylation in maize. Plant Cell https://doi.org/10.1105/
TY - JOUR. T1 - Epigenetic genes regulated by the BRAFV600E signaling are associated with alterations in the methylation and expression of tumor suppressor genes and patient survival in melanoma. AU - Liu, Dingxie. AU - Liu, Xuan. AU - Xing, Michael Mingzhao. PY - 2012/8/17. Y1 - 2012/8/17. N2 - We have previously reported that the BRAFV600E signaling causes genome-wide aberrations in gene methylation in melanoma cells. To explore the potential molecular mechanisms for this epigenetic effect of BRAFV600E, in this in silico study we analyzed 11 microarray datasets retrieved from NCBI GEO database and examined the relationship of the expression of the epigenetic genes (genes involved in epigenetic regulation) with BRAFV600E signaling, methylation and expression of tumor-suppressor genes (TSGs) in melanoma, and patient survival with this cancer. Among 273 epigenetic genes examined, 12 genes were down-regulated (named DD genes) and 16 were up-regulated (UU genes) by suppression of the BRAFV600E ...
The most enjoyable part in following RNAi Therapeutics is to look at the rich stream of scientific data and determine the absolute maturity and competitive position of the technologies and companies involved, as well as getting a glimpse at relationship dynamics. I therefore thought to share today two examples of this that I picked up recently. One is a paper by Sirna Therapeutics/Merck shedding some light on their approach towards RNAi pharmacology and RNAi trigger design. The other is some intriguing evidence that Silence Therapeutics most important gene target, PKN3, is gaining traction in the pharmaceutical space. Studying the pharmacology of siRNA delivery. Pei and colleagues from Merck published in RNA a nice paper on better understanding the pharmacology of siRNA delivery [Pei et al. (2010). Quantitative evaluation of siRNA delivery in vivo]. Unlike small molecules or even antibodies, the pharmacology of RNAi Therapeutics is more complex as simply measuring the raw tissue abundance of an ...
Author Summary Natural allelic variability is crucial for genetic improvement. While the genetic mechanisms leading to such variation have been studied in depth, relatively less is known about the role of epigenetic mechanisms in generation of allelic diversity. Paramutation is a phenomenon in which one allele can silence another allele in trans and, once established, such epigenetic silencing is heritable. To further understand the molecular components of paramutation, we characterized two epialleles of the pericarp color1 (p1) gene of maize, which originated from a common progenitor; however, only one of these alleles is paramutagenic. Results show that, while both alleles have high levels of symmetric (CG and CHG) methylation in a distal enhancer element, only the paramutagenic allele has higher levels of asymmetric (CHH) methylation. Since CHH methylation is imposed and maintained through RNA-mediated mechanisms, these results indicate that paramutation at the p1 locus involves RNA-mediated
Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint ...
Researchers at the Whitehead Institute and Memorial Sloan-Kettering Cancer Center have defined and analyzed the crystal structure of a yeast Argonaute protein bound to RNA. This complex plays a key role in the RNA interference (RNAi) pathway that silences gene expression. Describing the molecular structure of a eukaryotic Argonaute protein has been a goal of the RNAi field for close to a decade. You can learn a lot from biochemical experiments, but to more fully understand a protein like Argonaute, its useful to know where all of the atoms are and which amino acids are playing important roles, says Whitehead Institute Member Dr. David Bartel, who is also an MIT professor of biology and a Howard Hughes Medical Institute (HHMI) investigator. Learning the Argonaute crystal structure is an important step in understanding the RNAi biochemical pathway and will be the basis for many future experiments. The yeast Argonaute structure is described in the June 21, 2012 issue of Nature. In humans and ...
In recent years, the recognition of non-protein coding RNAs as a functional effector of genetic expression has been highlighted by the discovery of RNA interference (RNAi). RNAi is an intracellular phenomenon that enables the eukaryotic cell to utilize double-stranded RNA molecules to silence gene expression in a sequence-specific manner. The short interfering RNA (siRNA) pathway has been intensively investigated and continues to serve as the basis for the development of potent molecular genetic tools. The power of this technology is most clearly evidenced by the fact that siRNA effector molecules can be chemically synthesized and exogenously delivered to specifically target and silence any gene of choice. This capability enables not only basic research, but also opens the door to a new therapeutic modality. Furthermore, the introduction of certain chemical modifications to siRNA effectors can produce a more robust knockdown of gene expression, hence, optimizing serum stability and increasing target
Dr. Josephine Lai (Professor of Pharmacology, University of Arizona) is a pioneer in developing experimental designs and methods for delivering siRNA to the CNS for gene expression analysis.She and her team have documented these in the publication ...
The researchers determined that the protective effects of exosomes are mediated by microRNA, molecules that interfere with or silence gene expression. Further research is needed to understand more about the specific contents of the exosomes, said Stanislav Tomarev, Ph.D., a principal investigator at NEI and the studys coauthor.. We need to know which particular microRNA - there are more than 2000 different microRNA molecules - are delivered into the retinal ganglion cells and what proteins or signaling pathways are being targeted upon arrival, said Tomarev. We also need to attempt to target exosomes to specific sets of neurons and other cell types or groups of cells.. Finally, the most optimal exosome approach needs to be identified, Tomarev added. It may be that the best approach would be to combine exosomes with additional therapies. From a treatment feasibility standpoint, a lot will depend on how frequently exosomes need to be administered to achieve a therapeutic effect.. ...
With age, expression of microRNA-141-3p (small molecule) that can silence others goes way up while stromal-cell derived factor, or SDF-1 (key signaling molecule) helps ...
Anew detection mechanism for the control of successful siRNA delivery to cells or tissue is introduced using a siRNA-based probe that is capable of inducing a ...
Fig. 3. Chromocentres are located close to the nuclear periphery. (A) NucleusJ was used to compute the distance between the limit of the Hoechst DNA staining (blue) and the chromocentres (Cc, pink), boundary [d(Cc border)] or barycentre [d(Cc barycentre)]. The barycentre of the nucleus d(Nuc barycentre) (white cross) is also indicated. (B) Graphical representation of chromocentre distribution in respect to the limit of Hoechst DNA staining among the three cell types. The theoretical uniform distribution of chromocentres (top) is compared to observed distributions for d(Cc border) (middle) and d(Cc barycentre) (bottom). The uniform distribution of chromocentres is obtained by placing the same number of chromocentres as in the corresponding datasets between the periphery and the corresponding nuclear barycentres, for each nucleus of the dataset. Chromocenters and nuclei numbers are given at the bottom of the figure. The scales of the graphs were standardized by setting the maximum d(Nuc ...
Sigma-Aldrich offers abstracts and full-text articles by [Zhang, DD; Wang, WT; Xiong, J; Xie, XM; Cui, SS; Zhao, ZG; Li, MJ; Zhang, ZQ; Hao et al.].
IMO I think that people go with siRNA first to see if their siRNA sequence works and you get good knockdown. If you get good knowndown, then you can make the shRNA plasmid. But if you dont see good knockdown you end up making a new siRNA. If you went to shRNA first and then found that there is no knowndown, then it is a waste of time and money.. ...
chemical synthesized siRNA and rapidly dividing cells - posted in siRNA, microRNA and RNAi: Hi all, I am new to RNAi area. Your suggestion and opinion are very important to me If i am to use some rapidly dividing cells (e.g. HEK 293 cells) to perform RNAi study, is it not wise to use chemically synthesized siRNA? I have this wonder because in this case the RNAi effects may be too insignificant to be detectable. Is my concept correct? since i think for chemically synthesized siRNA, i...
Short interfering RNAs (siRNAs) are a popular method for gene-knockdown, acting by degrading the target mRNA. Before performing experiments it is invaluable to locate and evaluate previous knockdown experiments for the gene of interest.
We have recently identified HOP hoemobox (HOPX) as a tumor suppressor gene candidate, characterized by tumor-specific promoter DNA hypermethylation in human cancers, and it can remarkably inhibit tumors aggressive phenotypes. In this current study, we for the first time examined methylation level of HOPX and tested the functional relevance in pancreatic cancer (PC). Clinical features of HOPX promoter hypermethylation was investigated in 89 PC tissues, and immunohistochemistry was added. We also examined its functional relevance in phenotype assays such as soft agar, proliferation, invasion, and cell cycle analysis. PC tissues had HOPX gene hypermethylation as compared to the corresponding normal pancreas tissues, and its uniqueness was robust to discriminate tumor from normal tissues (AUC = 0.85, P | 0.0001). Unexpectedly, HOPX was increased in expression in tumor tissues, and immunohistochemistry revealed its predominant expression in the Langerhans islet cells, where HOPX was reduced in expression
Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic ...
TY - CHAP. T1 - Short hairpin RNA-mediated gene silencing. AU - Lambeth, Luke S. AU - Smith, Craig A.. PY - 2013. Y1 - 2013. N2 - Since thefirst application of RNA interference (RNAi) in mammalian cells, the expression of short hairpin RNAs (shRNAs) for targeted gene silencing has become a benchmark technology. Using plasmid and viral vectoring systems, the transcription of shRNA precursors that are effectively processed by the RNAi pathway can lead to potent gene knockdown. The past decade has seen continual advancement and improvement to the various strategies that can be used for shRNA delivery, and the use of shRNAs for clinical applications is well underway. Driving these developments has been the many benefits afforded by shRNA technologies, including the stable integration of expression constructs for long-term expression, infection of difficultto-target cell lines and tissues using viral vectors, and the temporal control of shRNA transcription by inducible promoters. The use of different ...
TY - JOUR. T1 - Methylation-associated silencing of the heat shock protein 47 gene in human neuroblastoma. AU - Yang, Qiwei. AU - Liu, Shuqing. AU - Tian, Yufeng. AU - Hasan, Chiler. AU - Kersey, Donna. AU - Salwen, Helen R.. AU - Chlenski, Alexandre. AU - Perlman, Elizabeth J.. AU - Cohn, Susan L.. PY - 2004/7/1. Y1 - 2004/7/1. N2 - Hypermethylation of gene promoter CpG islands is a frequent mechanism for gene inactivation in a variety of human cancers, including neuroblastoma (NB). We demonstrated recently that treatment with the demethylating agent 5′-aza-2′-deoxycytidine (5-Aza-dC) significantly inhibited NB growth in vivo. In an effort to identify the genes and biological pathways that are responsible for the impaired NB tumor growth observed after treatment with 5-Aza-dC, we performed genome-wide gene expression analysis of control and treated NBL-W-S NB cells. We found ≥2-3-fold changes in expression of 44 genes that play roles in angiogenesis, apoptosis, cell adhesion, ...
Regulation of gene transcription is central to developmental processes. By establishing and maintaining specific patterns of transcription, various pathways are co-ordinated for correct development of the organism. Primary control occurs through interactions between specific regulatory DNA sequences and a large variety of transcription factors. A higher order of control occurs through regulation of chromatin states. Position effect variegation in Drosophila is a well known example of how gene expression can be affected by chromatin organisation (Lu and Eissenberg, 1998). Heterochromatin-associated protein 1 (HP1) of Drosophila, is one of the key components in this phenomenon, and is involved in the generation and maintenance of an inactive heterochromatin structure that silences gene expression (Eissenberg and Elgin, 2000). Cell differentiation or developmental processes such as embryogenesis, also require chromatin modifications to maintain repression of homeotic genes mediated by ...
The present invention is directed to methods and materials for RNA-mediated gene assembly from oligonucleotide sequences. In some embodiments, the oligonucleotides used for gene assembly are provided in an array format. An RNA polymerase promoter is appended to surface-bound oligonucleotides and a plurality of RNA copies of each oligonucleotide are then produced with an RNA polymerase. These RNA molecules self-assemble into a desired full-length RNA transcript by hybridization and ligation. The resulting RNA transcript may then be converted into double strand DNA useful in a variety of applications including protein expression.
During meiosis in the filamentous fungus Neurospora crassa, unpaired genes are identified and silenced by a process known as meiotic silencing by unpaired DNA (MSUD). Previous work has uncovered six proteins required for MSUD, all of which are also essential for meiotic progression. Additionally, they all localize in the perinuclear region, suggesting that it is a center of MSUD activity. Nevertheless, at least a subset of MSUD proteins must be present inside the nucleus, as unpaired DNA recognition undoubtedly takes place there. In this study, we identified and characterized two new proteins required for MSUD, namely SAD-4 and SAD-5. Both are previously uncharacterized proteins specific to Ascomycetes, with SAD-4 having a range that spans several fungal classes and SAD-5 seemingly restricted to a single order. Both genes appear to be predominantly expressed in the sexual phase, as molecular study combined with analysis of publically available mRNA-seq datasets failed to detect significant ...
DNA methylation is an important epigenetic modification that serves to protect the genome from propagating mutations, and to regulate gene expression. Aberrant...
Several years ago, I posted a blog about long noncoding RNAs (lncRNAs), which are defined as non-protein coding transcripts in the range of ~200 nt to ~100 kb long. Interest in lncRNA is driven in large part by a collective scientific desire to uncover and understand the existence and function of all forms of RNA dark matter, so named by analogy to dark energy in cosmology. The lncRNA component of RNA dark matter is certainly generated from transcription of noncoding (formerly junk) DNA, but much has yet to be elucidated about function.. Following are tag lines from my Jan 23, 2018 blog titled Long Noncoding RNA (lncRNA) Revisted, which provides some updates on lncRNA dark matter.. ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Meiotic silencing by unpaired DNA is an RNAi-mediated pathway that functions to silence unpaired genes during the sexual phase of Neurospora crassa. The presence of a gene insertion or deletion causes an unpairing event during the homolog pairing stage. My dissertations aim was to further characterize this new RNAi pathway. First, I was involved in adapting the in vivo protein interaction technique Bimolecular Fluorescence Complementation (BiFC) to N. crassa to investigate SAD-1/SAD-2 interaction. SAD-1 and SAD-2 interact in the perinuclear region, suggesting that SAD-2 functions to bring SAD-1 to its proper location. Next, I was involved in identifying components of quelling that also function in meiotic silencing. dcl-1 and qip were found to be important for sexual development and meiotic silencing. DCL-1 and QIP also localize in the perinuclear region, implicating these genes in meiotic silencing. Finally, I tested the mating type locus for immunity to meiotic silencing. Unpaired reporter ...
Circulating Long Noncoding RNA as a Potential Target for Prostate Cancer. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Long Noncoding RNA LINC01485 Promotes Tumor Growth and Migration via Inhibiting EGFR Ubiquitination and Activating EGFR/Akt Signaling in Gastric Cancer
In the present study, we showed that a mild cooling of the cortex reversibly eliminated silent states and led to persistent thalamocortical activity in lightly anesthetized mice. Spontaneous IPSPs within the VPM neurons disappeared during cooling-induced cortical activation. Similarly, mild cortical cooling prevented the generation of cortical silent states in non-anesthetized head-restrained mice. Under the lesser physiological condition of deep anesthesia, a moderate cooling of the cortex reduced slow-wave synchrony but did not prevent the generation of slow waves. It also evoked fast cortical LFP spikes with frequencies ranging from 6 to 9 Hz, which correlated to spindle-like IPSPs within the VPM neurons and depolarizing events within the PO neurons. Our data point to a strong cortical control of thalamic activities.. The essential finding of this study is that a moderate cortical cooling in lightly anesthetized or naturally sleeping mice transforms the slow-wave pattern of neuronal activity ...
But to continue, w/that caveat in mind. Authorities attempt to silence the noise-swarm of the masses, and when it comes through, it does not speak in their language. The radio is a model for this kind of communication: EVP, voice phenomena, white-noise voices, static, interference - the message that scrambles, distorts, and replaces the original, clean communication, that is contained within it, yet that goes against it, that seems to come, and perhaps does come, from outside it, as attack. On the one hand, this implies that all voices are equivalent, that by turning the dial, you can silence one voice, push them back into the noise of radio noise, come to the next clean channel, the lush strings that drown out the loud scream. Its a matter of choice, in other words. Yet there are those moments when the radio interference becomes uncontrollable, resists the desire to remain on the safe channel. Voices in other languages, other dialects, bursts and blarts of discord break through. The voices ...
For both deals, Cenix will combine its high-throughput RNAi-based gene-silencing technology with high-content phenotypic analyses of genes in cultured human cells to uncover and validate in vitro certain undisclosed drug candidates.
In normal cells, DNA methylation is rare in promoter-associated CpG islands but important to X-inactivation, genomic imprinting and repression of repetitive ele...
siRNA transfection is a powerful tool used to understand the mechanisms of gene regulation and molecular pathways. The following 10 tips will help you to optimize your siRNA transfection.
Cancer has long been viewed as a genetic disease; however, epigenetic silencing as the result of aberrant promoter DNA methylation is frequently associated with cancer development, suggesting an epigenetic component to the disease. Nonetheless, it has remained unclear whether an epimutation (an aberrant change in epigenetic regulation) can induce tumorigenesis. Here, we exploited a functionally validated ...
In mammalian cells the introduction of small 21-23 nucleotide sequence-specific RNA duplexes (small/short interfering RNAs, siRNAs) can act to initiate post-transcriptional gene knockdown and avoid triggering non-specific effects in mammalian cells
Benenson and colleagues engineered a target gene to be sensitive to several different siRNAs of their own design. In the simplest case, they introduced a single siRNA molecule to switch off a target gene that encoded a fluorescent protein. In more complex cases, a pair of siRNAs or either of two siRNAs switched off another target gene, which in turn switched off a gene for a fluorescent protein. To make sure the system worked as intended, the researchers based their siRNAs on those of other species, they report in a paper published online today by Nature Biotechnology ...
The first in a new class of gene-silencing drugs, known as inclisiran, has been shown to halve cholesterol levels in patients at risk of cardiovascular disease.
Accell siRNA has a higher working concentration than siGENOME or ON-TARGETplus siRNA products. Use this tool as a guideline for ordering the appropriate amount of Accell siRNA for your cell culture format. http: //dharmacon. horizondiscovery. com/resources/tools-and-calculators/sirna-calculators ...
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目前从事棉花与害虫互作分子机制研究,主要通过多重组学(转录组、蛋白质组,代谢组等)手段,挖掘棉花自身的抗虫防御相关基因,利用高效的植物基因编辑技术体系验证相关基因的功能。同时分离鉴定多种外源的植物抗虫基因、抗性机制(新型BT基因、凝集素基因,RNAi等),通过细胞核转化和叶绿体转化表达体系,提高植物的抗虫性。. ...