Blood 2016 128(22); 2685. Background: The chromosomal translocation t(7;11)(p15,p15) encodes the oncogenic transcription factor NUP98-HOXA9 which results in a fusion of the nucleoporin 98kDa (NUP98) and homeobox A9 (HOXA9) genes. The oncogenic mechanisms underlying this translocation remain poorly understood and patients are currently inadequately served by traditional cytotoxic chemotherapy regimens.. Aims:To decipher the underlying biology of the NUP98-HOXA9 fusion protein and develop rational therapeutic strategies targeting its oncogenic mechanism. Methods: Human cellular models expressing NUP98-HOXA9, HOXA9 wt or NUP98 wt were established by retroviral transduction of HEK293FT human cell line and human hematopoietic progenitors (CD34+, hHP) isolated from donor cord blood. Chromatin immunoprecepitation experiments followed by sequencing (ChIP-seq) and quantitative ChIP (qChIP) were used to define fusion specific binding locations. Cloning regulatory regions of selected target genes in a ...

Scientists have long known that the protein p53, when mutated, is a critical factor in the onset of many different kinds of cancer. In its unmutated form, however, it is known to protect against cancer.

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Cancer pathways can potentially improve patient outcomes and reduce costs. Recent concerns about pathway adoption deserve attention, including excessive administrative burden to clinics.

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TY - JOUR. T1 - Integrative analysis of cancer pathway progression and coherence. AU - Dalkic,Ertugrul. AU - Walter Nash,Daniel Elwin. AU - Fassia,Mohammad Kasim. AU - Chan,Christina. PY - 2009. Y1 - 2009. N2 - We analyzed the cancer pathways in the KEGG (Kyoto Encyclopedia of Genes and Genomes) database. The database provides a collective of signaling pathway members involved in cancer progression. However, the KEGG cancer pathways, unlike signaling pathways, have not been analyzed extensively with gene expression and mutation data. We transformed the colorectal cancer pathway into discrete X and Y scales and analyzed the relative expression levels of adenoma and carcinoma samples as well as the distribution of mutation targets. The X scale corresponds to the downstream location in a pathway, whereas the Y scale corresponds to the stage of the tumor. The gene expression values of the early stage pathway members are significantly higher than of the rest of the pathway members in colorectal ...

Colorectal cancer (CRC) is the most common cancer and a leading cause of death worldwide. Extracellular matrix (ECM) proteins regulate tumor growth and development in CRC. Elastin (ELN) is a component of ECM proteins involved in the tumor microenvironment. However, the role of ELN in CRC remains unclear. In this study, we analyzed ELN gene expression in tumors from CRC patients and adjacent non-tumor colon tissues and healthy controls from two existing microarray datasets. ELN protein was measured in human normal colon cells and colon cancer epithelial cells and tumor development was assessed in colon epithelial cells cultured in medium with or without ELN peptide on plates coated with ELN recombinant protein. Control plates were coated with PBS only. We found ELN gene expression was increased in tumors from CRC patients compared to adjacent non-tumor tissues and healthy controls. ELN protein was increased in cancer cells compared to normal colon epithelial cells. Transforming growth factor beta (TGF-β

Breast cancer gene expression tests analyze the RNA of multiple genes in a breast tumor. The results are used to help predict the likely course of the cancer and to guide treatment.

PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.

הבידוד של תאי גזע סרטני (CSCs) ישירות מרקמות אנושיות הוא הנדרש לאפיון הביולוגי שלהם. כתב יד זה ...

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Comparison of gene expression signatures across molecular subtypes. Box plots of expression metagenes and scores across molecular subtypes: luminal, proliferati

Top 10 cell-lines for Q9ULL0 (Homo sapiens, UniProt): MX-1, NCI-H1963, SK-MEL-2, UACC-812, MCF-7-con, NCI-H1048, MCF-7-TP53 KD, NC-37, NALM-1, Hs 695T

Because 4.1B expression is frequently downregulated in human clinical prostate cancer (and a spectrum of other tumor types), these 4.1B mutant mice may be useful in studying the role of 4.1B as a negative regulator of cancer progression to metastatic disease.

Top 10 cell-lines for Q96LK0 (Homo sapiens, UniProt): My-La CD4+, NCI-H1581, Sez-4, A2058, NCI-H841, MFE-280, MFE-296, SK-UT-1B, U-251MG-PARK2, Kasumi-6

Introduction: Case management (CM) has been proposed as a method for optimizing the course of treatment for complicated cancer patients. However evidenc

Disabling a single enzyme associated with the formation of lipids significantly disrupts the ability of aggressive cancer cells to spread and grow tumors, according to a new study.

The present study investigated the clinical significance and biological role of MFN2 in bladder cancer pathogenesis. First, the expression level of MFN2 was revealed to be significantly lower in bladder cancer tissue compared with adjacent non-tumor tissues. Second, MFN2 expression level was identified to be associated with tumor stage, lymph node metastasis and poor prognosis in patients with bladder cancer. Third, silencing of MFN2 promoted bladder cancer proliferation and metastasis via regulation of the Wnt/β-catenin signaling pathway. These results indicated that MFN2 is a candidate tumor suppressor in bladder cancer, and may be exploited as a target for potential clinical treatments for bladder cancer.. MFN2, also termed the hyperplasia suppressor gene, was originally identified in vascular smooth muscle cells from spontaneously hypertensive rats (15). Overexpression of MFN2 induces the formation of mitochondrial networks and may involve a major rearrangement of the coiled coil domains ...

TY - JOUR. T1 - Hypomethylation of the 14-3-3σ promoter leads to increased expression in non-small cell lung cancer. AU - Radhakrishnan, Vijayababu M.. AU - Jensen, Taylor J.. AU - Cui, Haiyan. AU - Futscher, Bernard W.. AU - Martinez, Jesse D.. PY - 2011/10. Y1 - 2011/10. N2 - The 14-3-3 proteins are a set of seven highly conserved proteins that have recently been implicated in having a role in human tumorigenesis. However, the mechanism by which 14-3-3 proteins may act in this capacity is not well understood. In this study, we examined the expression of one of the 14-3-3 family members, 14-3-3σ, since it was shown previously to be aberrantly altered in human tumors. Using quantitative rtPCR and immunohistochemistry, we found that the expression levels of 14-3-3σ were elevated in the majority of human non-small cell lung cancers (NSCLC) we examined. Surprisingly, we found that the 14-3-3σ gene was hypomethylated in lung tumors relative to normal lung tissue suggesting that decreased DNA ...

Gene expression profiling classifies individual tumors by their gene expression patterns and may also describe and predict therapeutic resistance and sensitivity patterns. Profiling in several cancers, such as breast cancer, colon cancer, lymphoma, leukemia, and melanoma [3], has already identified molecular subclasses of tumors. Identification of tumor subtypes may be predictive for prognosis or response to drug therapy [6, 7, 28-31].. The potential of routine gene expression profiling to predict clinical outcomes for cancer patients has yet to be determined. The Evaluation of Genomic Applications in Practice and Prevention Working Group stated in 2009 that there was insufficient evidence to make a recommendation for or against the use of tumor gene expression profiles to improve outcomes in defined populations of women with breast cancer [32]. Clearly, more work needs to be done to translate promising research findings into clinically relevant results.. Comparison of FFPET sample-derived ...

As a disease with extremely complex molecular mechanisms, many deregulated miRNAs have been identified in colon cancer. Few studies have been performed by using Ingenuity Pathways Analysis (IPA) to predict miRNAs specifically expressed in colon cancer. A characteristic microRNA-target network of colon cancer was explored using IPA. Then the clinical significance of miR-19b-3p was evaluated in 211 colon cancer patients. The roles of miR-19b-3p and its candidate target gene, SMAD4, in colon cancer progression were examined both in vitro and in vivo. Bioinformatics analysis showed that 15 microRNAs screened by IPA were significantly correlated with malignant biological behaviors of colon cancer. miR-19b-3p was the most significantly upregulated candidate based on the validation experiment using 211 colon cancer samples. High expression of miR-19b-3p was significantly associated with high N stage (P | 0.001), high AJCC stage (P | 0.001), poor histologic grade (P = 0.032), frequent venous and lymphatic

As a disease with extremely complex molecular mechanisms, many deregulated miRNAs have been identified in colon cancer. Few studies have been performed by using Ingenuity Pathways Analysis (IPA) to predict miRNAs specifically expressed in colon cancer. A characteristic microRNA-target network of colon cancer was explored using IPA. Then the clinical significance of miR-19b-3p was evaluated in 211 colon cancer patients. The roles of miR-19b-3p and its candidate target gene, SMAD4, in colon cancer progression were examined both in vitro and in vivo. Bioinformatics analysis showed that 15 microRNAs screened by IPA were significantly correlated with malignant biological behaviors of colon cancer. miR-19b-3p was the most significantly upregulated candidate based on the validation experiment using 211 colon cancer samples. High expression of miR-19b-3p was significantly associated with high N stage (P | 0.001), high AJCC stage (P | 0.001), poor histologic grade (P = 0.032), frequent venous and lymphatic

MicroRNAs (MiRNAs) are short non-coding RNAs that control protein expression through various mechanisms. Their altered expression has been shown to be associated with various cancers. The aim of this study was to profile miRNA expression in colorectal cancer (CRC) and to analyze the function of specific miRNAs in CRC cells. MirVana miRNA Bioarrays were used to determine the miRNA expression profile in eight CRC cell line models, 45 human CRC samples of different stages, and four matched normal colon tissue samples. SW620 CRC cells were stably transduced with miR-143 or miR-145 expression vectors and analyzed in vitro for cell proliferation, cell differentiation and anchorage-independent growth. Signalling pathways associated with differentially expressed miRNAs were identified using a gene set enrichment analysis. The expression analysis of clinical CRC samples identified 37 miRNAs that were differentially expressed between CRC and normal tissue. Furthermore, several of these miRNAs were associated with

TY - JOUR. T1 - Oncogenic pathway combinations predict clinical prognosis in gastric cancer. AU - Ooi, Chia Huey. AU - Ivanova, Tatiana. AU - Wu, Jeanie. AU - Lee, Minghui. AU - Tan, Iain Beehuat. AU - Tao, Jiong. AU - Ward, Lindsay. AU - Koo, Jun Hao. AU - Gopalakrishnan, Veena. AU - Zhu, Yansong. AU - Cheng, Lai Ling. AU - Lee, Julian. AU - Rha, SunYoung. AU - Chung, Hyuncheol. AU - Ganesan, Kumaresan. AU - So, Jimmy. AU - Soo, Khee Chee. AU - Lim, Dennis. AU - Chan, Weng Hoong. AU - Wong, Wai Keong. AU - Bowtell, David. AU - Yeoh, Khay Guan. AU - Grabsch, Heike. AU - Boussioutas, Alex. AU - Tan, Patrick. PY - 2009/10/1. Y1 - 2009/10/1. N2 - Many solid cancers are known to exhibit a high degree of heterogeneity in their deregulation of different oncogenic pathways. We sought to identify major oncogenic pathways in gastric cancer (GC) with significant relationships to patient survival. Using gene expression signatures, we devised an in silico strategy to map patterns of oncogenic pathway ...

Detail záznamu - Neoplastic progression of the human breast cancer cell line G3S1 is associated with elevation of cytoskeletal dynamics and upregulation of MT1-MMP - Detail záznamu - Knihovna Akademie věd České republiky

The heterogenic nature of tumor cell lines makes it impossible to ascribe all aspects of a more aggressive or less aggressive phenotype to one molecule and that the MSLN/miR-198 interaction may not be solely responsible for the entirety of the aggressive phenotype. We have observed that while MIA-PaCa2 cells or Panc-1 cells are inherently more aggressive than other cell lines, forced overexpression of MSLN in these cell lines leads to an even more significantly aggressive phenotype. The reverse effect can be seen in cell lines with high endogenous MSLN expression, such as ASPC1, which, while they may be inherently less aggressive than others, become even less invasive and proliferate more slowly if MSLN expression is artificially reduced. Loss of miR-198 may be indicative to aggressiveness of pancreatic cancer based on our data obtained from several human pancreatic cancer cell lines, which are commonly used in the scientific community. However, it could be a limitation that we did not include ...

Considerable scientific evidence suggests that physical activity reduces the risk of several cancer types with the evidence classified as convincing or probable for colon, breast, and endometrial cancers; possible for prostate, ovarian, and lung cancers; and null or insufficient for other cancers.

Colorectal cancer (CRC) is the third most prevalent cancer in the developed countries, accounting for more than 50,000 cancer deaths per year. We used microarray to analyze gene expression in patients with different stages of colorectal cancer. Among the 157 metastasis-related genes, RT-PCR and quantitative real-time PCR (qPCR) were used to verify 11 genes. The roles of anillin (ANLN), one of 11 metastasis-associated genes, in colorectal cancer are not clear. Therefore, we focused on ANLN in this study. We found that the expression levels of anillin are higher in tumor specimens, even in metastasis tumor grade compared with those of adjacent normal tissues by real-time PCR. In order to understand the roles of anillin in tumorigenesis and metastasis of colorectal cancer, we used anillin overexpressed HT29 and shRNA-knockdowned SW480. Our data indicated that anillin overexpressed HT29 showed faster replication kinetics probably due to prolonged G2/M phase. Next, we found that the number and size ...

Using laboratory and mouse models of human breast cancer, researchers have found that a small molecule capable of targeting specific proteins on the surface of breast cancer cells can inhibit the growth of breast cancer cells that migrate to the brain. The small molecule used in the studies was the drug lapatinib (Tykerb), which disrupts an important breast cancer metabolic

Next-generation sequencing has uncovered thousands of long noncoding RNAs (lncRNA). Many are reported to be aberrantly expressed in various cancers, including hepatocellular carcinoma (HCC), and play key roles in tumorigenesis. This review provides an in-depth discussion of the oncogenic mechanisms reported to be associated with deregulated HCC-associated lncRNAs. Transcriptional expression of lncRNAs in HCC is modulated through transcription factors, or epigenetically by aberrant histone acetylation or DNA methylation, and posttranscriptionally by lncRNA transcript stability modulated by miRNAs and RNA-binding proteins. Seventy-four deregulated lncRNAs have been identified in HCC, of which, 52 are upregulated. This review maps the oncogenic roles of these deregulated lncRNAs by integrating diverse datasets including clinicopathologic features, affected cancer phenotypes, associated miRNA and/or protein-interacting partners as well as modulated gene/protein expression. Notably, 63 deregulated ...

The article Effects of long non-coding RNA URHC on proliferation, apoptosis and invasion of colorectal cancer cells, by Z.-G. Gu, G.-H. Shen, J.-H. Lang, W.-X. Huang, Z.-H. Qian, J. Qiu, published in Eur Rev Med Pharmacol Sci 2018; 22 (6): 1658-1664-DOI: 10.26355/eurrev_201803_14577-PMID: 29630109 has been withdrawn from the authors. The Publisher apologizes for any inconvenience this may cause. httpss://www.europeanreview.org/article/14577. ...

DECEMBER 22, 2008-- According to researchers in the US and Japan, a new type of molecular imaging agent enables visualization of viable breast cancer cells that have spread to the lungs in mice. The compound binds to a protein found on the surface of some breast cancer cells, and it fluoresces only when taken inside living cells--thus overcoming a limitation of other probes. The approach has enabled detection of various live cancer cell types in a mouse model.

Cervical cancer cell. Coloured scanning electron micrograph (SEM) of a cervical cancer cell. This large rounded cell has an uneven surface with many cytoplasmic projections, which may enable it to be motile. Typically, cancer cells are large and they divide rapidly in a chaotic manner. Cancer cells may clump to form tumours which may invade and destroy surrounding tissues. Cancer of the cervix (the neck of the uterus) is one of the most common cancers affecting women and can be fatal. - Stock Image M850/0450

Supplementary MaterialsSupp info. immunohistochemistry analysis shows that H2Bub1 is extremely low or undetectable in 70% of 170 lung adenocarcinoma samples. Notably, statistical analysis demonstrates that loss of H2Bub1 is usually significantly correlated with poor differentiation in lung adenocarcinoma (= 0.0134). In addition, sufferers with H2Bub1-harmful cancers got a craze towards shorter success compared with sufferers with H2Bub1-positive malignancies. Taken jointly, our findings claim that lack of H2Bub1 may enhance malignancy and promote disease development in lung adenocarcinoma most likely through modulating multiple tumor signaling pathways. reported an 11-gene personal including AZD4547 irreversible inhibition USP22 mRNA is certainly associated with intense growth, metastasis, and therapy level of resistance in a genuine amount of individual malignancies, including lung tumor.27 One research showed that knockdown of USP22 decreased cell proliferation in a number of cancers cell ...

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The gene-expression profile we studied is a more powerful predictor of the outcome of disease in young patients with breast cancer than standard systems based on clinical and histologic criteria.

Chemokines and their receptors have been detected in most tumors (1, 2). CCL2 remains one of the best studied chemokines. It has been shown that CCL2 may play a role in prostate cancer tumorigenesis and metastasis (4, 5). CCL2 is not only involved in inflammatory responses, but also may stimulate prostate cancer cell chemoattraction, proliferation, and survival (6, 7). Overexpression of CCL2 in human prostate cancer cells significantly increased local tumor burden in vivo (24, 25). Other in vivo data further showed that CCL2-neutralizing antibodies effectively inhibit prostate cancer growth (26). SNPs located in the regulatory region of the gene may increase or decrease transcriptional activity and polymorphisms affecting the gene encoding proteins may affect the function of encoded protein, potentially leading to the association with disease susceptibility or severity (15, 27). Therefore, it is biologically plausible that genetic polymorphisms that increase CCL2 expression may be associated ...

Recent studies suggest that thousands of genes may contribute to breast cancer pathophysiologies when deregulated by genomic or epigenomic events. Here, we describe a model system to appraise the functional contributions of these genes to breast cancer subsets. In general, the recurrent genomic an …

Research published in Nature Communications demonstrates a test for the detection of cancer that requires 10 minutes or less to analyse.. Prof Paul Pharoah, Professor of Cancer Epidemiology at the University of Cambridge, said: This paper is too preliminary to be exciting.. In short they have an interesting technology that can be used to determine patterns of methylation of DNA. And they have evidence that those patterns differ in cancer tissue compared to normal tissue.. It is already known that the DNA methylation pattern in cancer tissues differ from those of normal tissues, but the assays to show this are not easy to perform as a simple test. What they have shown is that their test can distinguish the DNA methylation in cancer tissues and normal tissues. They compared 72 samples from several cancers with 31 normal tissue samples to show this.. However, there are many issues with this study that limit its interpretation and it is clearly too preliminary to state that it could be a game ...

addition, samples of genomic DNA of human brain, breast, liver, testis, leukocytes, and breast tumor tissues, were purchased from BioChain. Preparation of RNA Cells were rinsed twice with 1 PBS. Total RNA was extracted according to the RNeasy Mini Kit Spin Protocol ...

Accumulating evidence has recently revealed that the dysregulation of lncRNAs serves an essential role in the development and invasion of human cancer, including CRC (20,21). The present study revealed that SNHG22 expression was significantly elevated in CRC tissues and cell lines. High levels of SNHG22 expression were significantly associated with unfavorable clinicopathological characteristics and worse survival in patients with CRC. Functionally, ectopic SNHG22 overexpression drove proliferation, apoptosis resistance, migration and invasion in CRC cell lines. Knocking down SNHG22 inhibited xenograft tumor growth in vivo. The present study confirmed that SNHG22 performed its tumor-promoting function by sponging miR-128-3p, and enhancing the expression and activity of E2F3. To the best of our knowledge, the present results are the first to clarify that SNHG22 acts as an oncogene in CRC, and that it may be used as a potential therapeutic target for this disease.. The current anatomically based ...

Researchers Use Microarray-Based Gene Expression Technology to Help Classify Tumor Profiles and Stratify Treatment Options NEW YORK--(BUSINESS WIRE)-- ChipDX LLC, a New York-based online molecular

miR-126 were over-expressed using the miR-Vec system in highly metastatic LM2 cells. The LM2 cell line are described in detail in Minn et al. Nature 2005 This approach was used to conduct an unbiased search for specific miR-126 target genes in breast cancer cells. 4 Samples

DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.

Fingerprint Dive into the research topics of Garcinol sensitizes breast cancer cells to Taxol through the suppression of caspase-3/iPLA,sub,2,/sub, and NF-κB/Twist1 signaling pathways in a mouse 4T1 breast tumor model. Together they form a unique fingerprint. ...

The studies presented here reveal that high level BP1 expres sion is associated with enhanced survival of breast cancer cells challenged with TNF

4) Haferlach T et al. - Clinical utility of microarray-based gene expression profiling in the diagnosis and subclassificati... - J Clin Oncol - ...

Pancreatic cancer cell lines with varying degrees of genetic complexity, including mutations in FBXW7, RAS, MAP2K4, SMAD4, and TP53.

Pancreatic cancer cell lines with varying degrees of genetic complexity, including mutations in FBXW7, RAS, MAP2K4, SMAD4, and TP53.

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon. ...

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon. ...

Explore the biology of a cancer cell, follow cancer pathways, and learn the molecular basis of diagnostics and treatments. Inside Cancer is a multimedia site for teachers, students, and family members who want authoritative information on the biology of a cancer cell.

Researchers have identified an enzyme that is absent in healthy colon tissue but abundant in colon cancer cells. The enzyme appears to drive the conversion of normal colon tissue into cancer by attaching sugar molecules, or glycans, to certain proteins in the cell.

Critical Outcome Technologies Inc. released important new test results today proving that COTI-2, the companys lead oncology candidate, stops cancer cells from replicating by correcting the effects of ...

Dive into the research topics of Inactivation of cancer-associated-fibroblasts disrupts oncogenic signaling in pancreatic cancer cells and promotes its regression. Together they form a unique fingerprint. ...

Zhao JJ, Lin J, Lwin T, Yang H, Guo J, Kong W, Dessureault S, Moscinski LC, Rezania D, Dalton WS, Sotomayor E, Tao J, Cheng JQ; microRNA expression profile and identification of miR-29 as a prognostic marker and pathogenetic factor by targeting CDK6 in mantle cell lymphoma.; Blood, 2010 PubMed Europe PMC ...

Zhao JJ, Lin J, Lwin T, Yang H, Guo J, Kong W, Dessureault S, Moscinski LC, Rezania D, Dalton WS, Sotomayor E, Tao J, Cheng JQ; microRNA expression profile and identification of miR-29 as a prognostic marker and pathogenetic factor by targeting CDK6 in mantle cell lymphoma.; Blood, 2010 PubMed Europe PMC Scholia ...

A discovery that cancer cell lines can be used to predict how a tumor is likely to respond to a drug has implications for developing new, personalized treatments.

BACKGROUND: The Cancer/Testis Antigens (CTAs) are a heterogeneous group of proteins whose expression is typically restricted to the testis. However, they are aberrantly expressed in most cancers that have been examined ...

Cancer biology and biosynthesis , Cancer biology and biosynthesis , کتابخانه الکترونیک و دیجیتال - آذرسا