A novel role of interleukin-1-converting enzyme in cytokine-mediated inducible nitric oxide synthase gene expression: Implications for neuroinflammatory disease
Arterial baroreflex sensitivity is attenuated in chronic heart failure (CHF) state, which is associated with cardiac arrhythmias and sudden cardiac death in patients with CHF. Our previous study showed that CHF-induced sodium channel dysfunction in the baroreceptor neurons was involved in the blunted baroreflex sensitivity in CHF rats. Mitochondria-derived superoxide overproduction decreased expression and activation of the sodium channels in the baroreceptor neurons from CHF rats. However, the molecular mechanisms responsible for the sodium channel dysfunction in the baroreceptor neurons from CHF rats remain unknown. We tested the involvement of nuclear factor κB (NFκB) in the sodium channel dysfunction and evaluated the effects of in vivo transfection of manganese superoxide dismutase gene and NFκB shRNA on the baroreflex function in CHF rats. CHF was developed at 6 to 8 weeks after left coronary artery ligation in adult rats. Western blot and chromatin immunoprecipitation data showed that ...
Sigma-Aldrich offers abstracts and full-text articles by [F Cournarie, D Azzout-Marniche, M Foretz, C Guichard, P Ferre, F Foufelle].
The present study highlights the following novel findings on the mechanisms responsible for the normalization of endothelial dysfunction by calcium antagonists. (1) The calcium antagonist nifedipine indirectly upregulates SOD activity and expression in ECs through activation of adjacent VSMCs. (2) VEGF released from VSMCs is involved in the mechanism underlying the upregulation of endothelial SOD activity by nifedipine. (3) Nifedipine stimulates the release of VEGF from VSMCs through activation of the bradykinin B2 receptor. (4) Upregulation of endothelial SOD by nifedipine results in the enhancement of NO production from ECs.. Calcium antagonists are widely used in the treatment of hypertension and angina pectoris. Recent evidence suggests that these drugs improve clinical outcome in patients with certain cardiovascular diseases.12,13⇓ It is noteworthy that calcium antagonists have been shown to normalize endothelial dysfunction in many cardiovascular diseases,14 because endothelial ...
The Drosophila nitric-oxide synthase gene (dNOS) encodes a family of proteins that can modulate NOS activity by acting as dominant negative regulators ...
Recently we have shown that in vitro binding of the proximal part of the human tyrosine hydroxylase gene to the nuclear matrix is correlated with its transcriptional activity. The strongest binding potential was predicted by computing for the first intron sequence (Lenartowski & Goc, 2002, Neurosci Lett.; 330 : 151-154). In this study a 16 kb fragment of the bovine genomic DNA containing the tyrosine hydroxylase gene was investigated for its affinity to the nuclear matrix. Only a 950 bp fragment encoding the distal part of the first intron, second exon and a few nucleotides of the second intron bound to the nuclear matrix. The binding was independent of the tissue-specific tyrosine hydroxylase gene activation. The fragment was subcloned and sequenced. Computer search pointed to one potential intronic matrix attachment region with two AP1-like sites embedded in the sequence. We conclude that even if the position of the matrix binding region is conserved among the tyrosine hydroxylase genes in ...
in Experimental Cell Research (2001), 265(1), 114-24. Hypoxia is an important pathophysiological stress that occurs during blood vessel injuries and tumor growth. It is now well documented that hypoxia leads to the activation of several transcription factors ... [more ▼]. Hypoxia is an important pathophysiological stress that occurs during blood vessel injuries and tumor growth. It is now well documented that hypoxia leads to the activation of several transcription factors which participate in the adaptive response of the cells to hypoxia. Among these transcription factors, AP-1 is rapidly activated by hypoxia and triggers bFGF, VEGF, and tyrosine hydroxylase gene expression. However, the mechanisms of AP-1 activation by hypoxia are not well understood. In this report, we studied the events leading to AP-1 activation in hypoxia. We found that c-jun protein accumulates in hypoxic HepG2 cells. This overexpression is concomitant with c-jun phosphorylation and JNK activation. Moreover, we showed ...
Renalase山羊多克隆抗体(ab31291)可与小鼠, 人样本反应并经WB, ELISA, IHC实验严格验证,被3篇文献引用。所有产品均提供质保服务,中国75%以上现货。
Curcumin inhibits nitric oxide synthase gene expression. Curcumin is a naturally occurring, dietary polyphenolic phytochemical that has been shown to inhibit cancer among other things. With respect to inflammation, it inhibits the activation of free radical activated transcription factors, and reduces the production of proinflammatory cytokines such as tumor necrosis factor-alpha (TNF), interleukin-1 and interleukin-8). Upon inflammation, an enzyme is induced (nitric oxide synthase) that catalyzes the production of nitric oxide (NO), a molecule that may lead to carcinogenesis. In this study in mouse immune cells curcumin reduced the production of nitric oxide in a concentration-dependent manner. Furthermore, curcumin reduced nitric oxide expression in the livers of mice by 50-70%. Investigators were able to obtain potency at nanomoles per gram of body weight, even though it is believed that curcumin needs to be given at dosages that are unattainable through diet to produce an in vivo effect. ...
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Background: In diabetic retinopathy, the vascular endothelium is damaged due to oxidative stress and inflammation, and vitreous VEGF concentration becomes elevated. The association of diabetic retinopathy with single nucleotide polymorphisms (SNPs) was studied on two genes: VEGF, an important mediator of neovascularisation, and MnSOD, a major antioxidant enzyme.. Methods: The study population was 755 individuals consisting of 131 diabetic (type 1 or type 2) patients with diabetic retinopathy (DR group), 98 diabetic controls without retinopathy (DC group) and 526 non-diabetic controls. VEGF SNPs rs699947, rs2010963, rs2146232, rs3025033, rs3025039 and Ala16Val polymorphism of the MnSOD gene were genotyped.. Results: The frequencies of allele and genotype of the single genotyped VEGF SNPs or reconstructed haplotypes of these single SNPs did not differ between DR and DC groups. A higher frequency of the AlaAla genotype (p = 0.03) and Ala16 allele (p = 0.04) of the MnSOD gene in the DR group was ...
The irreversible destruction of the tissues that comprise synovial joints is the hallmark of both rheumatoid arthritis and osteoarthritis. In both diseases, inflammatory cytokines, such as interleukin-1β, stimulate the production of matrix metalloproteinases (MMPs), a family of enzymes that collectively degrade all components of the extra-cellular matrix. The collagenases, a subgroup of the MMP family, have the unique ability to cleave the collagen fibrils that comprise cartilage, tendon, and bone, and thereby provide the rate-limiting step in the degradation of many joint structures. In the arthritides, MMP-1 and MMP-13 (collagenase-1 and collagenase-3, respectively) are key mediators of joint destruction, and therefore represent potential therapeutic targets. In this thesis, we identified the rexinoid LG100268 (LG268), a ligand for the nuclear hormone receptor (NHR) RXR, as a novel, selective inhibitor of MMP-1 and -13 expression, and investigated the molecular mechanisms behind its ...
The irreversible destruction of the tissues that comprise synovial joints is the hallmark of both rheumatoid arthritis and osteoarthritis. In both diseases, inflammatory cytokines, such as interleukin-1β, stimulate the production of matrix metalloproteinases (MMPs), a family of enzymes that collectively degrade all components of the extra-cellular matrix. The collagenases, a subgroup of the MMP family, have the unique ability to cleave the collagen fibrils that comprise cartilage, tendon, and bone, and thereby provide the rate-limiting step in the degradation of many joint structures. In the arthritides, MMP-1 and MMP-13 (collagenase-1 and collagenase-3, respectively) are key mediators of joint destruction, and therefore represent potential therapeutic targets. In this thesis, we identified the rexinoid LG100268 (LG268), a ligand for the nuclear hormone receptor (NHR) RXR, as a novel, selective inhibitor of MMP-1 and -13 expression, and investigated the molecular mechanisms behind its ...
Aromatase cytochrome P450, the key enzyme of estrogen biosynthesis from androgens, is encoded by CYP19. Its structure shows some peculiarities: exons II to X encode the protein, while multiple alternative exons I encode unique 5-untranslated regions of the aromatase mRNA transcripts. Immunohistochemistry studies in the rat have shown that pituitary aromatase expression is sex-dependent and varies across the estrous cycle, suggesting that estrogens might be involved in the regulation of aromatase activity and might act locally as a paracrine or autocrine factor in the pituitary. In the present study, we used RT-PCR to characterize aromatase transcripts and real-time PCR to quantify the expression of the total aromatase mRNA at the different stages of the estrous cycle and from an ovariectomy and estradiol replacement model. We identified the two previously described aromatase transcripts with a specific 5untranslated region of the brain 1f and the gonadal PII transcripts. Total aromatase mRNA
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Accepted name: 15-hydroxyprostaglandin dehydrogenase (NAD+). Reaction: (5Z,13E,15S)-11α,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11α-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+. Other name(s): NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (type I); PGDH; 11α,15-dihydroxy-9-oxoprost-13-enoate:NAD+ 15-oxidoreductase; 15-OH-PGDH; 15-hydroxyprostaglandin dehydrogenase; 15-hydroxyprostanoic dehydrogenase; NAD-specific 15-hydroxyprostaglandin dehydrogenase; prostaglandin dehydrogenase; 15-hydroxyprostaglandin dehydrogenase (NAD). Systematic name: (5Z,13E,15S)-11α,15-dihydroxy-9-oxoprost-5,13-dienoate:NAD+ 15-oxidoreductase. Comments: Acts on prostaglandin E2, F2α and B1, but not on prostaglandin D2. cf. EC 1.1.1.196 15-hydroxyprostaglandin-D dehydrogenase (NADP+) and EC 1.1.1.197 15-hydroxyprostaglandin dehydrogenase (NADP+).. Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9030-87-9. References:. 1. Änggård, E. and Samuelsson, ...
Profacgen provides professional enzyme activity assay service for the determination of enzyme activities and kinetics, facilitating better understanding of various roles enzymes play in physiological processes.
Inducible Nitric Oxide Synthase Expression and Luteal Cell DNA Fragmentation of Porcine Cyclic Corpora Lutea - Nitric Oxide Synthase;Corpora Lutea;Apoptosis;Estrous Cycle;
Although the evolution of multigene families involves multiple mechanisms, comprehensive analysis of phylogenetic tree and exon/intron gene structures, to a certain extent, allow us to make some generalizations and predictions about the possible origin of and relationships between different isoforms of Sus, as well as their possible function. Plant Sus proteins have been historically divided into at least three major groups (Sus1, SusA and New Group/NG) on the basis of phylogenetic tree and molecular structures analysis of their sequences [24, 29]. Phylogenetic analysis of cotton GaSus genes and other plant homologues in our work corroborated this classification (for unification and simplification, in this study, we renamed them as Sus I, II and III, respectively), and further support the idea that higher plant species may have at least one gene for each of the three groups [24]. The presence of five cotton Sus genes, GaSus1 to 5, in the Sus I group that cluster together with other dicot genes ...
Animal, Base-Sequence, Chromosome-Mapping, Crosses-Genetic, Enzyme-Induction, Genes-Structural, Human, Hybridization, Mice: ge, Mice-Inbred-C57BL, Mice-Inbred-NOD: ge, Molecular-Sequence-Data, Muridae: ge, Rats, Rats-Wistar, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S. ...
Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4 (By similarity).
Jung, A., Schlegel, W., Jackisch, R., Friedrich, E.J., Wendel, A., Rückrich, M.F.: Hoppe-Seylers Z. Physiol. Chem., 356, 787-798 (1975)PubMedCrossRefGoogle Scholar ...