GATA1 - GATA1 (untagged)- Human GATA binding protein 1 (globin transcription factor 1), 10ug available for purchase from OriGene - Your Gene Company.
GATA1 (phospho Ser310) antibody (GATA binding protein 1 (globin transcription factor 1)) for WB. Anti-GATA1 (phospho Ser310) pAb (GTX32395) is tested in Human samples. 100% Ab-Assurance.
The overlap amongst these organic processes and molecular features pinpointed 3 genes, C/EBP Gata2 (GATA binding protein 2), Foxg1 (forkhead box G1) (Fig. 7D).
cdna:novel chromosome:VEGA66:14:63198922:63245271:-1 gene:OTTMUSG00000032593 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Gata4 description:GATA binding protein 4 ...
GATA3 (phospho Ser308) antibody (GATA binding protein 3) for WB. Anti-GATA3 (phospho Ser308) pAb (GTX32396) is tested in Human, Mouse samples. 100% Ab-Assurance.
Affiliation:立教大学,理学部,教授, Research Field:Cell biology,Functional biochemistry,Cell biology, Keywords:ミトコンドリア,液胞型ATPase,オルガネラ,ATP,膜融合,組織特異的転写制御,GATA-GT1,品質管理,クロライドイオン,アポトーシス, # of Research Projects:21, # of Research Products:43, Ongoing Project:リン酸化修飾によるミトコンドリア機能と品質管理の制御機構
A Triad of Complete Dorsal Pancreatic Agenesis, Pancake Kidney and Bicornuate Uterus. An Association or an Incidental Finding: First Case in Literature, Sa
The extraembryonic endoderm of mammals is essential for nutritive support of the foetus and patterning of the early embryo. Visceral and parietal endoderm are major subtypes of this lineage with the former exhibiting most, if not all, of the embryonic patterning properties. Extraembryonic endoderm (XEN) cell lines derived from the primitive endoderm of mouse blastocysts represent a cell culture model of this lineage, but are biased towards parietal endoderm in culture and in chimaeras. Here, I further characterise XEN cells and show that these cell lines exhibit high levels of heterogeneity. In an effort for XEN cells to adopt visceral endoderm character different aspects of the in vivo environment were mimicked. I found that BMP4 and laminin promote a mesenchymal-to-epithelial transition of XEN cells with upregulation of epithelial markers and downregulation of mesenchymal markers. Gene expression analysis showed the differentiated XEN cells most resembled extraembryonic visceral endoderm. ...
GATA4 and GATA6 play essential, but redundant, roles in pancreas formation in mice and GATA6 mutations cause pancreatic agenesis in humans. Recently, GATA6 mutations have also been linked to adult-onset diabetes with subclinical or no exocrine insufficiency suggesting an important role for GATA6 in human ß cell physiology. To investigate the role of GATA6 in adult endocrine pancreas, we generated mice in which Gata6 is specifically inactivated in the pancreas. These mice develop glucose intolerance. Islets deficient in GATA6 activity display decreased insulin content and impaired insulin secretion. Gata6-deficient ß cells exhibit ultrastructural abnormalities including increased immature insulin granules, swollen mitochondria and disorganized endoplasmic reticulum. We also demonstrate that Pdx1 expression in adult ß cells depends on GATA sites in transgenic reporter mice and loss of GATA6 greatly impacts ß cell specific gene expression. These findings demonstrate the essential role of GATA6 ...
I did my PhD with Mark Ptashne, one of those molecular biologists I had read about in high school. I worked on basic mechanisms of transcription in yeast and mammals. Although this was a great time when we were trying to figure out how the black boxes of transcriptional activation (and repression) worked, I felt a need to explore these questions in a more biological context. So I went to Rosa Beddingtons laboratory. Rosa was a pioneer in early mouse development and she had just discovered a potential clue to how all mammals know how to distinguish between where to make their heads and tails. Work in her lab had uncovered a new anterior signaling centre in a little studied extra-embryonic endoderm lineage (visceral endoderm), previously thought to only play a support function in development. In Rosas lab in 1996, I needed an in vitro model that could be used for molecular biology that approximated the early embryo and extra-embryonic endoderm, and so I started working with ESCs. I honestly had ...
MalaCards based summary : Gata1-Related X-Linked Cytopenia is related to dyserythropoietic anemia and thrombocytopenia and porphyria, congenital erythropoietic. An important gene associated with Gata1-Related X-Linked Cytopenia is GATA1 (GATA Binding Protein 1). Affiliated tissues include bone, bone marrow and myeloid ...
Well, two causes of absent pancreas were known. Recessive mutations in the Pdx1 or Ptf1a genes had been shown to cause pancreatic agenesis. In these cases each parent had a bad copy of the gene and when the child inherited both bad copies, the pancreas didnt grow. But for Pdx1 mutations there was usually a family history of diabetes which we didnt have, and those cases were only missing the pancreas, not the gall bladder and had no problems with the heart or any other organ. For the Ptf1a cases they also had cerebellar agenesis, meaning they were missing a large part of their brain, sadly a fatal condition. Neither of these genes seemed plausible for Finlay. ...
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An yi ikirarin cewar karnukan Amurka sun fi samun gata ta fuskar samun maganin cutar sankara (cancer) fiye da yan Najeriya. Shin haka lamarin yake?
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Buy our GATA3 293T transfected lysate (positive control). ab94325 has been validated in western blot. Abcam now offers a 12-month guarantee.
Members of the GATA transcription factor family have been used in many transfection studies to investigate their roles in the regulation of gene expression. To correct for variations in transfection efficiency, the Renilla luciferase encoding plasmids pRL-TK and pRL-SV40 are commonly used as normalization controls. We report here that plasmids expressing GATA-4 or GATA-6 transcription factor increased Renilla luciferase gene expression by 2 to 8 fold when co-transfected with pRL-TK or pRL-SV40. This alteration of the control reporter gene activity was shown to cause erroneous normalization of transfection efficiency and thus misinterpretation of results in a transactivation assay. To circumvent the problem, we generated two mutant control plasmids from which putative GATA response elements were deleted. These deletions rendered pRL-SV40 unresponsive to GATA transcription factor stimulation and reduced the response of pRL-TK. A database search also indicates that consensus GATA binding sequences are
Formation of epiblast (EPI) - the founder line of all embryonic lineages - and extra-embryonic supportive tissues is one of the key events in mammalian development. The prevailing model of early mammalian development is based almost exclusively on the mouse. Here, we provide a comprehensive, stage-by-stage analysis of EPI and extra-embryonic primitive endoderm (PrE) formation during preimplantation development of the rabbit. Although we observed that rabbit embryos have several features in common with mouse embryos, including a stage-related initiation of lineage specification, our results demonstrate the existence of some key differences in lineage specification among mammals. Contrary to the current view, our data suggest that reciprocal repression of GATA6 and NANOG is not fundamental for the initial stages of PrE versus EPI specification in mammals. Furthermore, our results provide insight into the observed discrepancies relating to the role of FGF/ERK signalling in PrE versus EPI specification
An advantage of the zebrafish model is that embryos can survive a cardiomyopathy, which facilitates the analysis of other phenotypes (Heicklen-Klein et al., 2005). In addition to the heart, we find that Gata4 is required for formation of the intestine, liver, pancreas and swim bladder. Gata4/Gata5/Gata6 gene expression has been previously associated with endoderm-derived organs, and mouse knockout models showed specific functions for Gata4 in the foregut (Kuo et al., 1997; Molkentin et al., 1997) and gastric (Jacobsen et al., 2002) epithelium, and for Gata6 in the visceral endoderm (Morrisey et al., 1998) and lung epithelium (Yang et al., 2002). The zebrafish gata5 gene is also essential for gut morphogenesis and liver development, based on the analysis of the faust mutant (Reiter et al., 1999; Reiter et al., 2001), and depletion of Gata6 in Xenopus or zebrafish endoderm appears to disrupt normal intestinal morphogenesis (Peterkin et al., 2003). Therefore, all three of these Gata genes have ...
GATA1 - GATA1 Mutant (D218G), Myc-DDK-tagged ORF clone of Homo sapiens GATA binding protein 1 (globin transcription factor 1) (GATA1) as transfection-ready DNA available for purchase from OriGene - Your Gene Company.
GATA4 has been ascribed to a number of critical functions in the heart, spanning from the specification and differentiation of cardiac myocytes early in development to the regulation of the cardiac hypertrophic response in the adult. GATA4 mediates these processes through the direct transcriptional control of key cardiac structural and regulatory genes.1 For example, GATA4 has been shown to directly bind the promoters of the ANF, BNP, α-MHC, and β-MHC genes, thereby controlling their expression in the heart.1 Indeed, 3 of these 4 genes showed altered expression in the hearts of Gata4fl/flβMHC-Cre mice, validating the previously proposed proximal regulatory role of GATA4. Cardiac-specific deletion of Gata4 also resulted in the dysregulation of approximately 1.0% of all cardiac-expressed genes, further supporting the overall importance of GATA4 as a homeostatic regulator of gene expression in the heart.. GATA4 is also thought to function as a critical regulator of cardiac hypertrophy, although ...
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The role of GATA‐6 in cardiogenesis to date has been unclear. Although gain‐of‐function experiments suggested a role for this GATA factor in heart formation (Jiang and Evans, 1996; Gove et al., 1997; Charron et al., 1999), some loss‐of‐function data suggested that it may not be required at all except in the absence of GATA‐4 and ‐5 (Jiang et al., 1998; Morrisey et al., 1998; Koutsourakis et al., 1999). However, antisense studies demonstrated a requirement for GATA‐6 for specific gene expression during the differentiation of rat neonatal cardiomyocytes in culture (Charron et al., 1999). In this study, we demonstrate for the first time a crucial requirement for GATA‐6 in the maturation and maintenance of cardiomyocytes during embryonic development. We have employed a loss‐of‐function model using antisense MOs to study the function of GATA‐6. The depletion of GATA‐6 in Xenopus and zebrafish embryos causes a dramatic loss of heart tissue. We found that maturation of the ...
More than 30% of all human cancers contain activating mutations of the small G‐protein RAS. As a result of this, RAS has been intensely studied and many efforts have been made to identify pathways that sustain RAS‐driven transformation [1]. Recent studies have indicated that the transcription factor GATA2 is one of these partners in crime, but a mechanistic link between RAS and GATA2 had not been identified [2]. A paper in this issue of EMBO reports closes this gap showing that GATA2 can be activated by p38 in RAS‐transformed cells [3].. See also: KR Katsumara et al (September 2014) ...
Regulation of GATA1 gene expression by HIF1. (A) Real-time PCR analysis of HIF1A and GATA1 mRNA levels in K562 cells transfected with the HIF1α expression plas
This Is Article About Bokken, Katana Kayu, Tsuba Maru Gata Type 02 with Sageo (tali ito) Bokken dengan model Tsuba bentuk Moru Gata ini dilengkapi Sageo berwarna Hijau dan dilengkapi dengan ornamen-ornamen lainnya sehingga penampilannya mendekati kemiripan dengan pedang katana sungguhan. Disamping indah dipandang mata (cocok buat hiasan atau pajangan) juga sangat cocok untuk latihan seni beladiri samurai. Selain karena penampilannya yang indah, Sageo pada gagang… Selengkapnya ». Rating: 1.0 ...
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Our study reports five patients with GATA4 mutations and a variable phenotype of transient or permanent diabetes diagnosed in neonates or during childhood. The exocrine pancreatic phenotype ranged from complete agenesis to hypoplasia with subclinical exocrine insufficiency or normal exocrine function. Additional features included neurocognitive defects and congenital heart malformations. The variable phenotype was not correlated with the size of the deletion, consistent with the variable penetrance of GATA4 mutations/deletions reported in the literature. Both patients with pancreatic agenesis (this study and DAmato et al. [11]) had missense mutations rather than deletions, but the absence of diabetes in two heterozygous relatives suggests that a dominant-negative effect is unlikely (11). Our results indicate that GATA4 mutations/deletions are a rare cause of NDM, accounting for 0.5% of our international series (4/867 cases). The observation that postzygotic GATA4 mutations in embryonic heart ...
The mechanisms involved in the recognition of microbial pathogens and activation of the immune system have been extensively studied. However, the mechanisms involved in the recovery phase of an infection are incompletely characterized at both the cellular and physiological levels. Here, we establish a Caenorhabditis elegans-Salmonella enterica model of acute infection and antibiotic treatment for studying biological changes during the resolution phase of an infection. Using whole genome expression profiles of acutely infected animals, we found that genes that are markers of innate immunity are down-regulated upon recovery, while genes involved in xenobiotic detoxification, redox regulation, and cellular homeostasis are up-regulated. In silico analyses demonstrated that genes altered during recovery from infection were transcriptionally regulated by conserved transcription factors, including GATA/ELT-2, FOXO/DAF-16, and Nrf/SKN-1. Finally, we found that recovery from an acute bacterial infection ...
Zebrafish are capable of complete cardiac regeneration after resection of up to 20% of their apical myocardium or cryoinfarction throughout their whole life (Jopling et al, 2010). In contrast, mammals (the data are mainly from mice) can only effectively regenerate the heart during embryonic development or shortly after birth until P7, when myocyte proliferation ceases (Porrello et al, 2011). Indeed, the expression of cell cycle genes in the heart winds down within the first postnatal week, although transcriptional mechanisms of this phenomenon have remained elusive (Soonpaa et al, 1996). We demonstrated in this study that cardiac GATA4 becomes strongly downregulated at P7 and to a lesser extent also after cryoinjury in mice, whereas in zebrafish GATA4 expression is massively upregulated in cardiomyocytes in response to cardiac injury to enable regeneration.. GATA4 downregulation predisposes the neonatal mouse heart to defective regeneration as demonstrated by an increased scar size in the ...
BACKGROUND & AIMS: Patterning of the small intestinal epithelium along its cephalocaudal axis establishes three functionally distinct regions: duodenum, jejunum, and ileum. Efficient nutrient assimilation and growth depend on the proper spatial patterning of specialized digestive and absorptive functions performed by duodenal, jejunal, and ileal enterocytes. When enterocyte function is disrupted by disease or injury, intestinal failure can occur. One approach to alleviate intestinal failure would be to restore lost enterocyte functions. The molecular mechanisms determining regionally defined enterocyte functions, however, are poorly delineated. We previously showed that GATA binding protein 4 (GATA4) is essential to define jejunal enterocytes. The goal of this study was to test the hypothesis that GATA4 is sufficient to confer jejunal identity within the intestinal epithelium. METHODS: To test this hypothesis, we generated a novel Gata4 conditional knock-in mouse line and expressed GATA4 in the ...
BACKGROUND & AIMS: Patterning of the small intestinal epithelium along its cephalocaudal axis establishes three functionally distinct regions: duodenum, jejunum, and ileum. Efficient nutrient assimilation and growth depend on the proper spatial patterning of specialized digestive and absorptive functions performed by duodenal, jejunal, and ileal enterocytes. When enterocyte function is disrupted by disease or injury, intestinal failure can occur. One approach to alleviate intestinal failure would be to restore lost enterocyte functions. The molecular mechanisms determining regionally defined enterocyte functions, however, are poorly delineated. We previously showed that GATA binding protein 4 (GATA4) is essential to define jejunal enterocytes. The goal of this study was to test the hypothesis that GATA4 is sufficient to confer jejunal identity within the intestinal epithelium. METHODS: To test this hypothesis, we generated a novel Gata4 conditional knock-in mouse line and expressed GATA4 in the ...
Mesenchyme. Coalescence of the mesenchyme at the level where the dorsal pancreas will form is the first morphological sign of pancreatic development. Removal of the mesoderm, or the fibroblasts within the mesoderm, prior to pancreatic specification results in pancreatic agenesis (53, 107, 164). Mesoderm removal following specification results in a reduction of the total pancreatic size indicating an ongoing requirement for mesoderm signaling to attain complete organ development (94). Interestingly, culturing of pancreatic mesenchyme with other sections of the dorsal endoderm can promote pancreatic differentiation, while mesenchyme from other regions of the anterior-posterior axis does not have this ability (10). This suggests that the mesenchyme provides signals that promote pancreatic specification, yet limits differentiation, thereby allowing expansion of the organ. These signals come in a variety of sources.. Physical interactions between the mesoderm and developing pancreatic bud affect ...
GATA1 was first described as a transcription factor that activates the hemoglobin B gene in the red blood cell precursors of chickens.[30] Subsequent studies in mice and isolated human cells found that GATA1 stimulates the expression of genes that promote the maturation of precursor cells (e.g. erythroblasts) to red blood cells while silencing genes that cause these precursors to proliferate and thereby to self-renew.[31][32] GATA1 stimulates this maturation by, for example, inducing the expression of genes in erythroid cells that contribute to the formation of their cytoskeleton and that make enzymes necessary for the biosynthesis of hemoglobins and heme, the oxygen-carrying components of red blood cells. GATA1-inactivating mutations may thereby result in a failure to produce sufficient numbers of and/or fully functional red blood cells.[5] Also based on mouse and isolated human cell studies, GATA1 appears to play a similarly critical role in the maturation of platelets from their precursor ...
DataMed is a prototype biomedical data search engine. Its goal is to discover data sets across data repositories or data aggregators. In the future it will allow searching outside these boundaries. DataMed supports the NIH-endorsed FAIR principles of Findability, Accessibility, Interoperability and Reusability of datasets with current functionality assisting in finding datasets and providing access information about them.
Gene, Genes, Chromatin, Repression, Gene Expression, Transcription Factors, Transcription Factor, Association, DNA, Fog, Transcription Factor Gata1, Megakaryocytes, Erythroid Cells, Mice, Proteins, Regulation, Architecture, Cell Lineages, Mast Cells, Cell
T GATA2 is present at larger levels within the endothelial cells comprising LVV leaflets (E, arrows) compared with endothelial cells lining the jugular veins and jugular lymph sacs (E, arrowheads). GATA2 can also be present in cardiac valves (F ) and arterial endothelial cells (J ). Boxed region...
Expression of GATA1 (ERYF1, GATA-1, GF1, NF-E1, NFE1) in colon tissue. Antibody staining with HPA000232, HPA000233 and CAB009195 in immunohistochemistry.
Expression of GATA1 (ERYF1, GATA-1, GF1, NF-E1, NFE1) in liver tissue. Antibody staining with HPA000232, HPA000233 and CAB009195 in immunohistochemistry.
Gata. Am sperat, am daruit, am iubit, am uitat, am lasat, am gresit, am iertat. Am renuntat. Am fost mereu aici. Mi s-a luat. Pa.
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XenApp, XenDesktop, XenMobile and XenServer Xen® ailesinin bir parçasıdır.. © 1999-2020 Citrix Systems, Inc. Tüm Hakları Saklıdır.. ...
XenApp, XenDesktop, XenMobile and XenServer Xen® ailesinin bir parçasıdır.. © 1999-2020 Citrix Systems, Inc. Tüm Hakları Saklıdır.. ...
Introduction. In recent years the multipotent extraembryonic endoderm (XEN) stem cells have been a focus of intense research. In Vivo, XEN cells contribute to the formation of the extraembryonic endoderm, visceral and parietal endoderm and later on, the yolk sac. The mature mouse blastocyst consists of three distinct cell types: the trophectoderm, which gives rise to the trophoblast and extraembryonic ectoderm (ExEc), the pluripotent cells of the epiblast, and the primitive or extraembryonic endoderm (ExEn), an epithelial layer of cells on the surface of the epiblast. The primitive endoderm gives rise to: (i) visceral endoderm (VE) that surrounds the epiblast and the ExEc; and (ii) parietal endoderm (PE) that interacts with the trophoblast giant cell layer. PE cells migrate along the inner surface of the trophectoderm and together with trophoblast giant cells form the parietal yolk sac.. Description of Technology. This technology comprises a group of mouse cells induced in parallel to ...
Ver más] GATA transcription factors are expressed in the mesoderm and endoderm during development. GATA1-3, but not GATA4, are critically involved in hematopoiesis. An enhancer (G2) of the mouse Gata4 gene directs its expression throughout the lateral mesoderm and the allantois, beginning at embryonic day 7.5, becoming restricted to the septum transversum by embryonic day 10.5, and disappearing by midgestation. We have studied the developmental fate of the G2-Gata4 cell lineage using a G2-Gata4Cre;R26REYFP mouse line. We found a substantial number of YFP+ hematopoietic cells of lymphoid, myeloid and erythroid lineages in embryos. Fetal CD41+ /cKit+ /CD34+ and Lin- /cKit+ /CD31+ YFP+ hematopoietic progenitors were much more abundant in the placenta than in the aorta-gonad-mesonephros area. They were clonogenic in the MethoCult assay and fully reconstituted hematopoiesis in myeloablated mice. YFP+ cells represented about 20% of the hematopoietic system of adult mice. Adult YFP+ hematopoietic stem ...
Hematopoietic differentiation of the H1-GATA2−/− ES cell line. a CFUs of H1 or H1-GATA2−/− derived CD34+ cells. H1 or H1-GATA2−/− cells were co-cult
Introduction: GATA4 gene encodes a member of the GATA family of zinc-finger transcription factors. This protein is thought to regulate genes involved in...
Complete information for QRSL1 gene (Protein Coding), Glutaminyl-TRNA Synthase (Glutamine-Hydrolyzing)-Like 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The GATA1 gene is associated with X-linked GATA1-related cytopenia (MedGen UID: 335283) and X-linked Diamond-Blackfan anemia (MedGen UID: 266045).