Vertebrate hematopoietic stem cells are derived from vental mesoderm, which is postulated to migrate to both extra- and intraembryonic positions during gastrula and neurula stages. Extraembryonic migration has previously been documented, but the origin and migration of intraembryonic hematopoietic cells have not been visualized. The zebrafish and most other teleosts do not form yolk sac blood islands during early embryogenesis, but instead hematopoiesis occurs solely in a dorsal location known as the intermediate cell mass (IM) or Oellacher. In this report, we have isolated cDNAs encoding zebrafish homologs of the hematopoietic transcription factors GATA-1 and GATA-2 and have used these markers to determine that the IM is formed from mesodermal cells in a posterior-lateral position on the yolk syncytial layer of the gastrula yolk sac. Surprisingly, cells of the IM then migrate anteriorly through most of the body length prior to the onset of active circulation and exit onto the yolk sac. These ...
Down syndrome-associated acute megakaryoblastic leukemia (DS-AMKL) affects 1 in 500 children with DS and associates uniformly with somatic mutations in GATA1. DS-AMKL is often treated successfully; however, treatments can cause long-term side effects, such that mechanistic insights and targeted therapies are needed.. Recent studies report a recurring L340F mutation in DCAF7 in DS-AMKL, at the C-terminus of the protein. A highly conserved scaffold protein, DCAF7 is a cofactor for the chromosome 21-encoded kinase, DYRK1A, and shares 100% protein sequence homology among vertebrates. In our efforts to characterize the normal function of DCAF7 in hematopoiesis and its contribution to AMKL, we have identified an unreported PDZ-binding motif (PBM) in DCAF7, which is perturbed by the L340F mutation. Overexpression of mutant (DCAF7mut), but not wild type DCAF7 (DCAF7wt), in a mouse model of DS with a Gata1 mutation leads to rapid onset of dysplastic disease characterized by peripheral blood leukopenia ...
Definition of hematopoietic transcription factor PU.1. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013 ...
This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009 ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Study Subtype: Ancillary/Correlative Observational Study Model: Cohort Time Perspective: Retrospective Biospecimen Retention: Samples With DNA Biospecimen Description: Cryopreserved mRNA Study Population Description: Samples from AAML0531 Sampling Method: Non-Probability Sample. OBJECTIVES:. I. To determine whether NUP98/JARID1A expression is a recurrent translocation in NUP98-rearranged cases in pediatric acute megakaryoblastic leukemia (AMKL).. II. To screen the Children Oncology Group (COG) samples for genetic aberrations in pediatric AMKL.. OUTLINE:. Cryopreserved specimens are analyzed for NUP98 fusion to NSD1, JARID1A, and TOP1, myeloid/lymphoid or mixed-lineage leukemia (MLL)-rearrangements, and other gene expression profiling by reverse transcriptase polymerase chain reaction (RT-PCR) and karyotyping or fluorescence in situ hybridization (FISH). Results are then compared with each patients outcome data. ...
Recent evidence suggests that long-range enhancers and gene promoters are in close proximity, which might reflect the formation of chromatin loops. Here, we examined the mechanism for DNA looping at the beta-globin locus. By using chromosome conformation capture (3C), we show that the hematopoietic transcription factor GATA-1 and its cofactor FOG-1 are required for the physical interaction between the beta-globin locus control region (LCR) and the beta-major globin promoter. Kinetic studies reveal that GATA-1-induced loop formation correlates with the onset of beta-globin transcription and occurs independently of new protein synthesis. GATA-1 occupies the beta-major globin promoter normally in fetal liver erythroblasts from mice lacking the LCR, suggesting that GATA-1 binding to the promoter and LCR are independent events that occur prior to loop formation. Together, these data demonstrate that GATA-1 and FOG-1 are essential anchors for a tissue-specific chromatin loop, providing general insights into
AMKL is a heterogeneous subtype of AML generally associated with poor prognosis. Challenges in understanding the molecular basis of this disease derive in part from the difficulties in obtaining a sufficient amount of material, which together with the lack of experimental model limits the development of novel therapeutic strategies. Using a xenotransplantation approach with primary human AMKL patient samples into immunodeficient mice, we have established novel models of AMKL recapitulating features of the human disease. The leukemic blasts engrafted in immunodeficient recipient mice allowed the identification and characterization of novel genetic lesions and were relevant models to test the efficacy of AURKA inhibitors as a potential therapeutic strategy for the treatment of AMKL with various genetic alterations.. Xenotransplantation has been widely used as a strategy to propagate human leukemic cells in immunodeficient mice. To date however, no specific study of AMKL has been reported. In this ...
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Tumour suppressor gene function, focusing on several hematopoietic transcription factors recurrently mutated in acute lymphoblastic and myeloid leukemias (ALL and AML).. Cancer results from an accumulation of genetic mutations that disable the normal regulation of cell growth and survival. In the last decade, large scale cancer genome sequencing of all the major cancer types has identified ~150 genes that promote oncogenesis when mutated. To develop more effective cancer therapies, now the key challenge is to understand how these cancer driver genes, comprising a similar number of oncogenes and tumour suppressor genes, contribute to tumourigenesis and treatment response.. Our laboratory is interested in understanding tumour suppressor gene function, focusing on several hematopoietic transcription factors recurrently mutated in acute lymphoblastic and myeloid leukemias (ALL and AML). Our general approach uses shRNA-mediated inhibition of these transcription factors to drive leukemogenesis in ...
On the other hand, E‐boxes are absent from an unexpectedly high proportion of TAL1 peaks (14% in Jurkat and 24% in erythroid cells) (Figure 6C). Consistent with this, the de novo motif search did not identify E‐boxes as the top overrepresented motif in erythroid or Jurkat cells (Figure 6A, left and middle panels). Instead, in erythroid cells, a Gata motif ranks first within overrepresented sites, and two variants of this motif are also among the top 7 overrepresented motifs (Figure 6A, middle panel). Furthermore, virtually all TAL1 peaks (96%) contain a Gata motif while only 76% contain an E‐box within a 100‐bp radius of the peak summit (Figure 6C). In erythroid cells, Gata motifs are also on average closer to TAL1 peak summits than E‐boxes, with 80% of TAL1 peaks containing a Gata site within 35 bp of the peak summit versus only 50% containing an E‐box within this distance (Figure 6C). This is consistent with previous studies showing cooccupation of TAL1 and GATA1 at many genomic ...
GATA3 antibody for detecting human T-cell-specific transcription factor GATA-3. Validated on up to 12 cell lysates for western blotting. Try a trial size today.
Children with Down syndrome (DS) up to the age of 4 years are at a 150-fold excess risk of developing myeloid leukemia (ML-DS). Approximately 4%-5% of newborns with DS develop transient myeloproliferative disorder (TMD). Blast cell structure and immunophenotype are similar in TMD and ML-DS. A mutation in the hematopoietic transcription factor GATA1 is present in almost all cases. Here, we show that simple techniques detect GATA1 mutations in the largest series of TMD (n = 134; 88%) and ML-DS (n = 103; 85%) cases tested. Furthermore, no significant difference in the mutational spectrum between the 2 disorders was seen. Thus, the type of GATA1 sequence mutation is not a reliable tool and is not prognostic of which patients with TMD are probable to develop ML-DS.
Individuals with Down syndrome (DS) frequently have hematopoietic abnormalities, including transient myeloproliferative disorder and acute megakaryoblastic leukemia which are often accompanied by acquired GATA1 mutations that produce a truncated protein, GATA1s. The mouse has been used for modeling DS based on the syntenic conservation between human chromosome 21 (Hsa21) and three regions in the mouse genome located on mouse chromosome 10 (Mmu10), Mmu16 and Mmu17. To assess the impact of the dosage increase of Hsa21 gene orthologs on the hematopoietic system, we characterized the related phenotype in the Dp(10)1Yey/+;Dp(16)1Yey/+;Dp(17)1Yey/+ model which carries duplications spanning the entire Hsa21 orthologous regions on Mmu10, Mmu16 and Mmu17, and the Dp(10)1Yey /+; Dp(16)1Yey /+; Dp(17)1Yey/+ ; Gata1 Yeym2 model which carries a Gata1s mutation we engineered ...
The t(8;21) acute myeloid leukemia (AML)-associated oncoprotein AML1-ETO disrupts normal hematopoietic differentiation. Here, we have investigated its effects on the transcriptome and epigenome in t(8,21) patient cells. AML1-ETO binding was found at promoter regions of active genes with high levels of histone acetylation but also at distal elements characterized by low acetylation levels and binding of the hematopoietic transcription factors LYL1 and LMO2. In contrast, ERG, FLI1, TAL1, and RUNX1 bind at all AML1-ETO-occupied regulatory regions, including those of the AML1-ETO gene itself, suggesting their involvement in regulating AML1-ETO expression levels. While expression of AML1-ETO in myeloid differentiated induced pluripotent stem cells (iPSCs) induces leukemic characteristics, overexpression increases cell death. We find that expression of wild-type transcription factors RUNX1 and ERG in AML is required to prevent this oncogene overexpression. Together our results show that the interplay ...
Author: Dr. Beere Gowda , Dr. Suresh T.N., Dr. Kiran , Dr. Hemalatha. Category: Pathology. [Download PDF]. Abstract:. Congenital leukemia is a very rare disorder accounting for less than 1% of all childhood leukemias. Transient Myeloproliferative Disorder (TMD) occurs in 4 to 10% of infants with Down Syndrome (DS) characterized by numerous circulating blast cells and spontaneous resolution within a fewweeks. We present a 3day old male baby with DS presenting with clinical features of shock and high percentage of blast cells in peripheral smear. This case is presented to highlight the clinical and laboratory diagnostic dilemma.. Keywords: Down Syndrome (DS), TransientMyeloproliferative Disorder (TMD), TransientAbnormal Myelopoiesis (TAM), congenitalleukemia (CL) ...
GATA-1 and FOG-1 zinc fingers. Computer model showing the complex of the zinc finger transcription factors GATA-1 (orange) and FOG-1 (friend of GATA, blue). The two zinc ions shown in grey become visible after removing a few atoms hiding them. - Stock Image C035/5609
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:11154691). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1 and NFE2L2, and recruiting them to specific DNA-binding sites (PubMed:8932385, PubMed:9421508, PubMed:11154691). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NFE2L2 transcription factor (PubMed:11154691). Transcription factor, component of erythroid-specific transcription factor NFE2L2 (PubMed:11154691). Activates globin gene expression when associated with NFE2L2 (PubMed:11154691). May be involved in signal transduction of extracellular H(+) (By similarity).
Acute Megakaryoblastic Leukemia (AMKL) is a rare form of pediatric leukemia that disproportionately affects children. AMKL associated with the chromosomal translocation fusing RNA Binding Motif 15 gene (RBM15) on chromosome 1 upstream of the transcriptional cofactor Megakaryoblastic Leukemia 1 gene (MKL1) on chromosome 22 (t1;22), is most commonly diagnosed in infants less than three months of age and requires aggressive medical management. Thus, it is likely that the leukemia originates in utero when the hematopoietic system is in its embryonic or fetal stages. We have successfully recapitulated megkaryopoiesis in vitro using human embryonic stem cells and established a model system in which to study megakaryocyte differentiation. Human embryonic stem cells (hESCs) offer a mechanism to study embryogenesis and to understand the processes of fetal blood maturation and leukemia development. We seek to further define the roles of MKL1 and RBM15 during hESC-derived megakaryopoiesis. Furthermore, we ...
The present study uses highly purified T cells, Foxp3-GFP reporter mice, and analyses at the cellular and molecular level to reexamine the paradigm of local immune privilege in the eye. Our current data considerably extend what has been known about the suppressive ability of ocular fluids and provide new information on the likely fate of a T cell that enters the eye and undergoes TCR ligation in the ocular environment. The entire differentiation program for Th1 as well as for Th17 was shut down and diverted toward de novo Foxp3+ Treg induction. Interestingly, although phosphorylation of STAT1 and its target, the Th1 lineage-specific transcription factor T-bet, were both inhibited, phosphorylation of STAT3, which is triggered by IL-6R ligation and induces the Th17 lineage-specific transcription factor RORγt (29), was not affected, and neither was expression of IL-6Rα. This suggests that inhibition of RORγt by AH was not through the IL-6-induced STAT3 pathway. Because Foxp3 was shown to bind to ...
Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal hematopoietic disorders with a highly variable prognosis. To identify a gene expression-based classification of myelodysplasia with biological and clinical relevance, we performed a comprehensive transcriptomic analysis of myeloid neoplasms with dysplasia using transcriptome sequencing. Unsupervised clustering of gene expression data of bone marrow CD34+ cells from 100 patients identified two subgroups. The first subtype was characterized by increased expression of genes related to erythroid/megakaryocytic (EMK) lineages, whereas the second subtype showed up-regulation of genes related to immature progenitor (IMP) cells. Compared to the first, so-called EMK subtype, the IMP subtype showed up-regulation of many signaling pathways and down-regulation of several pathways related to metabolism and DNA repair. The IMP subgroup was associated with a significantly shorter survival in both univariate (hazard ratio [HR] 5.0 [95% confidence
GATA1 - GATA1 (untagged)- Human GATA binding protein 1 (globin transcription factor 1), 10ug available for purchase from OriGene - Your Gene Company.
cdna:novel chromosome:VEGA66:14:63198922:63245271:-1 gene:OTTMUSG00000032593 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Gata4 description:GATA binding protein 4 ...
Down syndrome (DS) children have a high frequency of acute megakaryoblastic leukemia (AMKL) in early childhood. At least 2 in utero genetic events are required, although not sufficient, for DS-AMKL: trisomy 21 (T21) and N-terminal-truncating GATA1 mutations. To investigate the role of T21 in DS-AMKL, we compared second trimester hemopoiesis in DS without GATA1 mutations to gestation-matched normal controls. In all DS fetal livers (FLs), but not marrows, megakaryocyte-erythroid progenitor frequency was increased (55.9% +/- 4% vs 17.1% +/- 3%, CD34(+)CD38(+) cells; P | .001) with common myeloid progenitors (19.6% +/- 2% vs 44.0% +/- 7%) and granulocyte-monocyte (GM) progenitors (15.8% +/- 4% vs 34.5% +/- 9%) commensurately reduced. Clonogenicity of DS-FL versus normal FL CD34(+) cells was markedly increased (78% +/- 7% vs 15% +/- 3%) affecting megakaryocyte-erythroid ( approximately 7-fold higher) and GM and colony-forming unit-granulocyte, erythrocyte macrophage, megakaryocyte (CFU-GEMM) progenitors.
A donation of cord blood from a little boy who died of leukemia is leading to key insights into this childhood cancer.. Acute leukemia is the most common cancer in children. There are several subtypes, including acute lymphoblastic leukemia (ALL), which has a high survival rate, and acute megakaryoblastic leukemia (AMKL), which is rare and has a very poor prognosis. Sometimes, leukemias coincide with a genetic mutation known as MLLr - mixed lineage leukemia gene rearrangement - which can make the disease more aggressive and difficult to treat.. American Cancer Society grantee Tanja Gruber, MD, PhD, is investigating what triggers leukemias with the MLL-rearrangement to develop, using a rare donation of cord blood. A California family had contacted her to see if she could use the cord blood from their son, Hendrix Wille, who had AMKL-MLLr.. "They saved his cord blood from birth. Before he died, they went through great lengths and broke through political red tape to make sure that I received his ...
Sæther, Thomas; Bengtsen, Mads; Molværsmyr, Ann-Kristin; Pattabiraman, D.R.; Alm-Kristiansen, Anne Hege; Ledsaak, Marit; Gonda, T.J. & Gabrielsen, Odd Stokke (2012). Regulating the regulator: SUMO functions as a master switch for the transcription factor c-Myb. Vis sammendrag The oncoprotein c-Myb is an essential hematopoietic transcription factor that controls proliferation and differentiation of progenitors during blood cell development. This transcriptional activator plays a direct role in lineage fate selection, cell cycle progression, and differentiation of myeloid as well as B- and T-lymphoid progenitors. Recent studies have shown that c-Myb accomplishes this by controlling the expression of several important hematopoietic transcriptions factors, and in that way acts as a master regulator. c-Myb is modified by SUMO on two lysines in its C-terminal regulatory domain (CRD), and is able to bind SUMO via a SUMO-interacting motif (SIM) in the central transactivation domain. Both these ...
Another form of leukemia, transient myeloproliferative leukemia, is identified with a heterozygous C to A transversion as well. In a 2002 leukemia journal written by Taketani et al., the RUNX1 gene was screened and studied in a sample group of 46 patients with down syndrome. These patients all had hematologic malignancies, meaning they were all affected by different cancers associated with bone marrow. Out of these patients, was identified with this C to A transversion and diagnosed with transient myeloproliferative leukemia 5 days after birth. However, the newborn patient died 12 months after birth. The newborn was never screened for acute myeloid leukemia. The conclusion here is that if there is an identified C-A mutation regarding the RUNX1 gene, then AML should be screened and tested for. An amniotic fluid test should be given to pregnant women in order to determine if their children carry the mutated gene associated with acute myeloid leukemia. http://omim.org/entry/151385#0008 ...
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Asthma is a highly prevalent airway disease, affecting an estimated 300 million people worldwide. Asthmatics suffer not only from the disease itself, but also from its wide-ranging consequences. The therapeutic options for severe asthma to date are little satisfactory and require a new therapeutic principle. This is what sterna biologicals has developed with the new drug "SB010". It is based on inhibiting the transcription factor GATA-3 by a so-called DNAzyme - a synthetic DNA molecule with enzymatic action for inhaled administration. It binds to the transcription factor that triggers the inflammatory response and thus the typical asthma symptoms, inactivating it by enzymatic cleavage.. The Fraunhofer ITEM has accompanied development of this first-in-class antagonist with its expertise, from scientific advice and the required toxicity tests (in cooperation with Nycomed GmbH) to clinical trials of phases Ib and IIa. The latter, i.e. the proof-of-concept study, was conducted as a multi-center ...
Buy our GATA3 293T transfected lysate (positive control). ab94325 has been validated in western blot. Abcam now offers a 12-month guarantee.
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Cell-Autonomous Function of Runx1 Transcriptionally Regulates Mouse Megakaryocytic Maturation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
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RNA Polymerase II (RNAP II) is recruited to core promoters by the pre-initiation complex (PIC) of general transcription factors. Within the PIC, transcription factor for RNA polymerase IIB (TFIIB) determines the start site of transcription. TFIIB binding has not been localized, genome-wide, in metazoans. Serial analysis of chromatin occupancy (SACO) is an unbiased methodology used to empirically identify transcription factor binding regions. In this report, we use TFIIB and SACO to localize TFIIB binding regions across the rat genome. A sample of the TFIIB SACO library was sequenced and 12,968 TFIIB genomic signature tags (GSTs) were assigned to the rat genome. GSTs are 20-22 base pair fragments that are derived from TFIIB bound chromatin. TFIIB localized to both non-protein coding and protein-coding loci. For 21% of the 1783 protein-coding genes in this sample of the SACO library, TFIIB binding mapped near the characterized 5 promoter that is upstream of the transcription start site (TSS). However,
The LIM only protein 2 (LMO2) is a key regulator of hematopoietic stem cell development whose ectopic expression in T cells leads to the onset of acute lymphoblastic leukemia. Through its LIM domains, LMO2 is thought to function as the scaffold for a DNA-binding transcription regulator complex, including the basic helix-loop-helix proteins SCL/TAL1 and E47, the zinc finger protein GATA-1, and LIM-domain interacting protein LDB1. To understand the role of LMO2 in the formation of this complex and ultimately to dissect its function in normal and aberrant hematopoiesis, we solved the crystal structure of LMO2 in complex with the LID domain of LDB1 at 2.4 Å resolution. We observe a largely unstructured LMO2 kept in register by the LID binding both LIM domains. Comparison of independently determined crystal structures of LMO2 reveals large movements around a conserved hinge between the LIM domains. We demonstrate that such conformational flexibility is necessary for binding of LMO2 to its partner ...
American Journal of Medical Genetics Part C (Seminars in Medical Genetics) 142C:149 - 157 (2006) A R T I C L E Clinical Manifestations of Hematologic and Oncologic Disorders in Patients With Down Syndrome NATALIA DIXON,* PRIYA S. KISHNANI, AND SHERRI ZIMMERMAN Hematologic abnormalities are common in individuals with Down syndrome (DS). Increased erythrocyte mean corpuscular volume (MCV) is frequently found among DS infants and remains elevated throughout life in twothirds of patients, making interpretation of red cell indices for diagnosis of nutritional anemias or bone marrow failure disorders more challenging. Transient myeloproliferative disorder (TMD) associated with pancytopenia, hepatosplenomegaly, and circulating immature WBCs, is found almost exclusively in DS infants with an incidence of approximately 10%. In most cases, TMD regresses spontaneously within the first 3 months of life, but in some children, it can be life threatening or even fatal. Despite the high rate of spontaneous ...
Transient myeloproliferative disorder and non-immune hydrops fetalis in a neonate with trisomy 21" (February 2014;20:78.e3-4). On page 78.e3 (3rd paragraph, lines 6-8), the sentence should have read "Rhesus isoimmunisation is the commonest immune aetiology, and alpha-thalassaemia is a non-immune cause." rather than "Rhesus isoimmunisation is the commonest immune aetiology, and beta-thalassaemia is the commonest non-immune cause." as printed. We regret the error. The article is correct at www.hkmj.org ...
We finally identified a set of 24 lncRNAs that showed differential expressions among the GC patients included in the data sets. Such differentiations signified their potential roles in GC. Although some of these deregulated lncRNAs have been reported to express in cancer or other disorders, they have not been investigated in GC. For example, the expression of AK026189 (CASC 15) was found to be associated with neurobalstoma and was increased during melanoma progression [57-59]. And it was regarded as an independent predictor of disease recurrence in a cohort of 141 patients with AJCC stage III lymph node metastasis [58]. In our study, AK026189 was highly expressed in GC and was found to be correlated with shortened survival. Another candidate, H04858 (MIR99AHG, MONC) was also abundantly expressed in GC samples. A study has revealed that H04858 was highly expressed in acute megakaryoblastic leukemia cell lines serving as a regulator of hematopoiesis and oncogene in the development of myeloid ...
The human ERYF1 gene (summary) NF-E1 DNA-binding protein GATA1, locus Xp11.23 [§§; †] containing 2 finger motifs referred to as ERYF1 of an erythroid-specific gene. The cDNA for the human ERYF1 gene is almost identical to that of chicken and mouse GATA1 gene consisting of 2 zinc finger type motifs its activator domain contains the binding…
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Hematopoietic differentiation of the H1-GATA2−/− ES cell line. a CFUs of H1 or H1-GATA2−/− derived CD34+ cells. H1 or H1-GATA2−/− cells were co-cult
More than 30% of all human cancers contain activating mutations of the small G‐protein RAS. As a result of this, RAS has been intensely studied and many efforts have been made to identify pathways that sustain RAS‐driven transformation [1]. Recent studies have indicated that the transcription factor GATA2 is one of these partners in crime, but a mechanistic link between RAS and GATA2 had not been identified [2]. A paper in this issue of EMBO reports closes this gap showing that GATA2 can be activated by p38 in RAS‐transformed cells [3].. See also: KR Katsumara et al (September 2014) ...
GATA1 - Gata1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector shRNA available for purchase from OriGene - Your Gene Company.
This Is Article About Bokken, Katana Kayu, Tsuba Maru Gata Type 02 with Sageo (tali ito) Bokken dengan model Tsuba bentuk Moru Gata ini dilengkapi Sageo berwarna Hijau dan dilengkapi dengan ornamen-ornamen lainnya sehingga penampilannya mendekati kemiripan dengan pedang katana sungguhan. Disamping indah dipandang mata (cocok buat hiasan atau pajangan) juga sangat cocok untuk latihan seni beladiri samurai. Selain karena penampilannya yang indah, Sageo pada gagang… Selengkapnya ». Rating: 1.0 ...
PRINCETON, N.J. & CAMBRIDGE, Mass.--(BUSINESS WIRE)--Bristol Myers Squibb (NYSE: BMY) and Acceleron Pharma Inc. (NASDAQ: XLRN) today announced the U.S.
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Tracheal Intubation in an Uncooperative Patient With a Neck Injury. In: Hung OR, Murphy MF. Hung O.R., Murphy M.F. Eds. Orlando R. Hung, and Michael F. Murphy.eds. Hungs Difficult and Failed Airway Management, 3e New York, NY: McGraw-Hill; . http://accessanesthesiology.mhmedical.com/content.aspx?bookid=2221§ionid=171845873. Accessed December 12, 2017 ...
Hypersensitive site 2 located in the beta-globin locus control region confers high levels of expression to the genes of the beta-globin cluster. A tandem repeat of the consensus sequence for the transcription factors AP1 and NF-E2 (activating protein 1 and nuclear factor erythroid 2, respectively) is present within hypersensitive site 2 and is absolutely required for strong enhancer activity. This sequence binds, in vitro and in vivo, to ubiquitous proteins of the AP1 family and to the recently cloned erythroid-specific transcription factor NF-E2. Using the tandem repeat as a recognition site probe to screen a lambda gt11 cDNA expression library from K562 cells, we isolated several DNA binding proteins. Here, we report the characterization of one of the clones isolated. The gene, which we named Nrf2 (NF-E2-related factor 2), is encoded within a 2.2-kb transcript and predicts a 66-kDa protein with a basic leucine zipper DNA binding domain highly homologous to that of NF-E2. Although Nrf2 is ...