Viruses infecting the Archaea exhibit a tremendous amount of morphological and genetic diversity. This is especially true for crenarchaeal viruses from the family Fuselloviridae, which possess spindle-shaped capsids and genomes that harbor a great number of uncharacterized genes. The functions of these unidentified gene products are of interest as they have the potential to provide valuable insights into the fusellovirus infection cycle and archaeal viruses in general. In an effort to better characterize the genetic requirements of the Fuselloviridae, we have performed genetic and biochemical experiments using the best studied fusellovirus, Sulfolobus spindle-shaped virus 1 (SSV1). A comprehensive genetic analysis of SSV1 was conducted using long inverse PCR and transposon mutagenesis. The results of this work illustrate that SSV1 is highly tolerant of mutagenesis. A robust protocol for the purification of recombinant VP2 protein from E. coli was developed and should be useful for future studies aimed

The basic replication cycle of crenarchaeal viruses, including Fuselloviruses, has yet to be determined. There are, however, some trends which have been reported. It is thought that members of the Fuselloviridae family, as well as members of the Rudiviridae, Lipothrixviridae, and Guttaviridae families, associate with host cells by tail fibers which are present on one or both ends of the virion. Some viruses integrate their genome into the host cells chromosome, while others maintain their genomes as extrachromosomal elements. Assembly and release of most known crenarchaeal viruses, do not require cell lysis. Most crenarchaeal viruses, twith the exception of the Bicaudaviridae member ATV, appear to set up chronic infections, either continually producing virus particles or doing so in short events resulting in growth inhibition. These long-term chronic infections are thought to be an adaptation to the extremely hot and acidic environments crenarchaeal viruses inhabit. (source: Ortmann et al.) ...

In hypersaline environments, haloarchaea (halophilic members of the Archaea) are the dominant organisms, and the viruses that infect them, haloarchaeoviruses are at least ten times more abundant. Since their discovery in 1974, described haloarchaeoviruses include head-tailed, pleomorphic, spherical and spindle-shaped morphologies, representing Myoviridae, Siphoviridae, Podoviridae, Pleolipoviridae, Sphaerolipoviridae and Fuselloviridae families. This review overviews current knowledge of haloarchaeoviruses, providing information about classification, morphotypes, macromolecules, life cycles, genetic manipulation and gene regulation, and host-virus responses. In so doing, the review incorporates knowledge from laboratory studies of isolated viruses, field-based studies of environmental samples, and both genomic and metagenomic analyses of haloarchaeoviruses. What emerges is that some haloarchaeoviruses possess unique morphological and life cycle properties, while others share features with other viruses

The remarkable diversity of the morphologies of viruses found in terrestrial hydrothermal environments with temperatures ,80°C is unprecedented for aquatic ecosystems. The best-studied viruses from these habitats have been assigned to novel viral families: Fuselloviridae, Lipothrixviridae and Rudiviridae. They all have double-stranded DNA genomes and infect hyperthermophilic crenarchaea of the orders Sulfolobales and Thermoproteales. Representatives of the different viral families share a few homologous ORFs (open reading frames). However, about 90% of all ORFs in the seven sequenced genomes show no significant matches to sequences in public databases. This suggests that these hyperthermophilic viruses have exceptional biochemical solutions for biological functions. Specific features of genome organization, as well as strategies for DNA replication, suggest that phylogenetic relationships exist between crenarchaeal rudiviruses and the large eukaryal DNA viruses: poxviruses, the African swine ...

For comparison, host of NSV, AOA, was quantified using primers for archaeal amoA gene with a previous protocol (Park et al., 2008). Archaeal amoA gene could be detected in all the samples as pPolB gene. pPolB and amoA gene copies were quantified as described previously (Kim et al., 2019), using real-time PCR with CFX Connect Real-Time system (Bio-Rad Laboratories) using iQ SYBRGreen Supermix (Bio-Rad Laboratories) and built-in CFX manager software v3.0 (Bio-Rad Laboratories). AamoAF and AamoAR primers (Francis et al., 2005) were used with following PCR condition: 95°C (5 min), then 40 cycles at 95°C (30 sec), 55°C (30 sec), and 72°C (1.5 min). For quantification of viral pPolB gene, semi-nested real-time PCR was performed: the first PCR using primers 620F and 1880R with following PCR condition: first, 95°C (10 min), then 20 cycles at 95°C (30 sec), 55°C (30 sec), and 72°C (1.5 min); the second PCR using primers 620F and primer 950R (5-CATGGCRTAWGGATAWGCWGAATT-3) with the same thermal ...

Electron microscopic studies of the viruses in two hot springs (85 degrees C, pH 1.5-2.0, and 75-93 degrees C, pH 6.5) in Yellowstone National Park revealed particles with twelve different morphotypes. This diversity encompassed known viruses of hyperthermophilic archaea, filamentous Lipothrixviridae, rod-shaped Rudiviridae, and spindle-shaped Fuselloviridae, and novel morphotypes previously not observed in nature. Two virus types resembled head-and-tail bacteriophages from the families Siphoviridae and Podoviridae, and constituted the first observation of these viruses in a hydrothermal environment. Viral hosts in the acidic spring were members of the hyperthermophilic archaeal genus Acidianus. ...

Woolly monkey sarcoma virus (WMSV), with synonym Simian sarcoma virus (often abbreviated by SSV, but this may also stand for some species called Sulfolobus spindle-shaped virus, that belong to different genera in family Fuselloviridae) is a species of gammaretrovirus that infects primates. First isolation was from a fibrosarcoma in a woolly monkey (genus Lagothrix). For its reproduction the virus needs a helper or associated virus which is called Simian sarcoma associated virus (SSAV). International Committee on Taxonomy of Viruses NCBI Taxonomy Browser Zoologix Simian sarcoma virus (SSV) and simian sarcoma associated virus (SSAV) University of Massachusetts Medical School Umass Profiles: Sarcoma Virus, Woolly Monkey S. G. Devare, E. Premkumar Reddy, J. Doria Law, K. C. Robbins, S. A. Aaronson: Nucleotide Sequence of the Simian Sarcoma Virus Genome: Demonstration that its Acquired Cellular Sequences Encode the Transforming Gene Product p28sis, JSTOR 13744, PMC 393453 E. P. Gelmannn, E. Petri, ...

GF ID SSV1_ORF_D-335 #=GF AC PF07935.10 #=GF DE ORF D-335-like protein #=GF AU Fenech M #=GF SE Pfam-B_4933 (release 14.0) #=GF GA 21.60 21.60; #=GF TC 22.10 44.60; #=GF NC 20.70 21.20; #=GF BM hmmbuild HMM.ann SEED.ann #=GF SM hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq #=GF TP Family #=GF RN [1] #=GF RM 1926776 #=GF RT Complete nucleotide sequence of the virus SSV1 of the #=GF RT archaebacterium Sulfolobus shibatae. #=GF RA Palm P, Schleper C, Grampp B, Yeats S, McWilliam P, Reiter WD, #=GF RA Zillig W; #=GF RL Virology 1991;185:242-250. #=GF DR INTERPRO; IPR012922; #=GF CC The sequences featured in this family are similar to a probable #=GF CC integrase (Swiss:P20214) expressed by the SSV1 virus of the #=GF CC archaebacterium Sulfolobus shibatae. This protein may be #=GF CC necessary for the integration of the virus into the host genome #=GF CC by a process of site-specific recombination [1]. #=GF SQ 17 #=GS Q980Y3_SULSO/40-112 AC Q980Y3.1 #=GS W7KM36_9CREN/1-47 AC W7KM36.1 #=GS ...

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The virions of members of the Guttaviridae have an ovoid shape, measuring 55 x 75 nm (for Aeropyrum pernix ovoid virus 1 (APOV1), Betaguttavirus) to 80 x 130 nm (for Sulfolobus newzealandicus droplet-shaped virus (SNDV), Alphaguttavirus), when analysed by cryo-electron microscopy [{Arnold et al., 2000:10873785RJOHTXArnold et al., 2000, SNDV, a novel virus of the extremely thermophilic and acidophilic archaeon Sulfolobus, Virology, 272, 2, 409-16RJOMREFMochizuki et al., 2011:21784945RJOHTXMochizuki et al., 2011, Provirus induction in hyperthermophilic archaea: characterization of Aeropyrum pernix spindle-shaped virus 1 and Aeropyrum pernix ovoid virus 1, J Bacteriol, 193, 19, 5412-9}]. The virion surface is covered by globular subunits, which are ~3.5 nm in width. In negative-contrast electron micrographs, the virions are slightly pleomorphic, most displaying a droplet-like shape. Virions of SNDV are decorated with dense filaments attached to the pointed end of the virion; such appendages were ...

Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids.

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

This is a blend of sauvignon blanc, semillon and a splash of viognier which is a creative move. And the viognier... David Lawrason - LCBO, SAQ, BCLDB

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General Information: This strain was isolated from a hot spring on the Kamchatka Penninsula, in the Russian Far East. Hyperthermophilic acidophilic sulfur-metabolizing archeon. Sulfolobus islandicus is a thermo-acidophilic archeae commonly identified in hot, acidic sulfur springs. This organism can grow both chemoautotrophically, using sulfur or hydrogen sulfide, and heterotrophically. S. islandicus can play host to a number of plasmids and viruses which may be useful in developing tools for genetic analysis. In addition, Sulfolobus islandicus isolates from different areas in Russia, Iceland, and the United States have been shown to be genetically distinct from each other making this organism useful for comparative analysis. ...

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The Island. The fact that abortion, or properly speaking induced abortion according to medical parlance, is always a contentious issue across the world goes without saying. That is what to be expected in a multiethnic, multi-religious, multicultural society like ours, where the views and opinions on matters such as these could be diverse as chalk and cheese. Further, an assortment of concerns ranging from moral, medical, religious and legal to fetal and womens rights and states authority impinge on the matter, thus making abortion one of the most intricate issues of our time. Needless to say, thus arriving at an all embracing consensus on the issue is a near impossibility, if not unrealistic. Naturally, there will be parties on either side of the divide, well armed with sound theoretical and philosophical ordnances, ready to fight tooth and nail to see that their viewpoint prevails at the end of the day. That could be what we are heading toward in the near future with regard to abortion, ...

ID SULID_1_PE2489 STANDARD; PRT; 366 AA. AC SULID_1_PE2489; D2PGF4; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=eIF-2B alpha/beta/delta-related uncharacterized protein; DE (SULID_1.PE2489). GN OrderedLocusNames=LD85_2679; OS SULFOLOBUS ISLANDICUS L.D.8.5. OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; OC Sulfolobus. OX NCBI_TaxID=425944; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS SULID_1.PE2489. CC Sulfolobus islandicus L.D.8.5 chromosome, complete genome. CC 1..88329 annotated by Ensembl Genomes CC -!- ANNOTATIONS ORIGIN:D2PGF4_SULID CC -!- SIMILARITY: Belongs to the eIF-2B alpha/beta/delta subunits CC family. CC -!- GENE_FAMILY: HOG000224730 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; D2PGF4; -. DR EMBL; CP001731; ADB88294.1; -; Genomic_DNA. DR RefSeq; YP_003420664.1; NC_013769.1. DR ProteinModelPortal; D2PGF4; -. DR GeneID; ...

However, Baas Becking has an answer for this; ...but the environment selects. What if the variation is due to selection acting at a finer scale? Its well established that species sorting effects play a major role in determining the composition of microbial communities at the species level. There is no particular reason to believe that this effect does not apply at smaller phylogenetic scales. The work with Sulfolobus islandicus attempts to control for this by choosing isolates from hot springs with similar physical and chemical properties, but unfortunately there is no such thing as a pair of identical hot springs. Just walk the boardwalks in Yellowstone, and youll see what I mean. The differences among the sites from which these microbes were isolated can always be offered as an alternative explanation to dispersal. Even if you crank those differences down to nearly zero, one can always suggest that perhaps there is a difference that we dont know about that happened to be important ...

SWISS-MODEL Repository entry for C3MZ62 (RISB_SULIA), 6,7-dimethyl-8-ribityllumazine synthase. Sulfolobus islandicus (strain M1627)

Dorsal soft rays (total): 37; Anal soft rays: 33; Vertebrae: 43. Coronal pore present. Lower pectoral fin rays longer and thicker than upper lobe rays. Gill rakers unmodified. Coracoid half-moon shaped, helve absent. Mouth short, almost vertical, about 75° to horizontal. Skin pale, head beneath skin unpigmented (Ref. 43481). ...

Background and Aim: To objectively assess splenic volume preoperatively, the Standard Splenic Volume (SSV) was calculated for each patient. Whether it..

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TY - JOUR. T1 - A broadly applicable gene knockout system for the thermoacidophilic archaeon Sulfolobus islandicus based on simvastatin selection. AU - Zhang, Changyi. AU - Whitaker, Rachel J.. PY - 2012/6. Y1 - 2012/6. N2 - Sulfolobus species have been developed as excellent model organisms to address fundamental questions of archaeal biology. Interesting patterns of natural variation among Sulfolobus islandicus strains have been identified through genome sequencing. Experimentally testing hypotheses about the biological causes and consequences of this natural variation requires genetic tools that apply to a diversity of strains. Previously, a genetic transformation system for S. islandicus was reported, in which overexpression of the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase gene on the shuttle vector pSSR allowed the selection of transformants resistant to high concentrations of the thermostable antibiotic simvastatin. Here, we developed a novel gene knockout system based on ...

ID SULID_1_PE1303 STANDARD; PRT; 195 AA. AC SULID_1_PE1303; D2PK04; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Glutamine amidotransferase of anthranilate synthase; DE (SULID_1.PE1303). GN OrderedLocusNames=LD85_1439; OS SULFOLOBUS ISLANDICUS L.D.8.5. OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; OC Sulfolobus. OX NCBI_TaxID=425944; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS SULID_1.PE1303. CC Sulfolobus islandicus L.D.8.5 chromosome, complete genome. CC 1..88329 annotated by Ensembl Genomes CC -!- ANNOTATIONS ORIGIN:D2PK04_SULID CC -!- GENE_FAMILY: HOG000025029 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; D2PK04; -. DR EMBL; CP001731; ADB87107.1; -; Genomic_DNA. DR RefSeq; YP_003419477.1; NC_013769.1. DR ProteinModelPortal; D2PK04; -. DR GeneID; 8761258; -. DR GenomeReviews; CP001731_GR; LD85_1439. DR KEGG; sii:LD85_1439; -. DR ...

ID RTCA_SULIM Reviewed; 337 AA. AC C3MU91; DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot. DT 16-JUN-2009, sequence version 1. DT 25-OCT-2017, entry version 52. DE RecName: Full=RNA 3-terminal phosphate cyclase {ECO:0000255,HAMAP-Rule:MF_00200}; DE Short=RNA cyclase {ECO:0000255,HAMAP-Rule:MF_00200}; DE Short=RNA-3-phosphate cyclase {ECO:0000255,HAMAP-Rule:MF_00200}; DE EC=6.5.1.4 {ECO:0000255,HAMAP-Rule:MF_00200}; GN Name=rtcA {ECO:0000255,HAMAP-Rule:MF_00200}; GN OrderedLocusNames=M1425_0235; OS Sulfolobus islandicus (strain M.14.25 / Kamchatka #1). OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; OC Sulfolobus. OX NCBI_TaxID=427317; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=M.14.25 / Kamchatka #1; RX PubMed=19435847; DOI=10.1073/pnas.0808945106; RA Reno M.L., Held N.L., Fields C.J., Burke P.V., Whitaker R.J.; RT Biogeography of the Sulfolobus islandicus pan-genome.; RL Proc. Natl. Acad. Sci. U.S.A. 106:8605-8610(2009). CC -!- FUNCTION: ...

Betalipothrixvirus is a genus of viruses in the order Ligamenvirales, in the family Lipothrixviridae. Archaea serve as natural hosts. There are currently six species in this genus including the type species Sulfolobus islandicus filamentous virus. Group: dsDNA Order: Ligamenvirales Family: Lipothrixviridae Genus: Betalipothrixvirus Acidianus filamentous virus 3 Acidianus filamentous virus 6 Acidianus filamentous virus 7 Acidianus filamentous virus 8 Acidianus filamentous virus 9 Sulfolobus islandicus filamentous virus Viruses in Betalipothrixvirus are enveloped, with rod-shaped geometries. The diameter is around 24 nm, with a length of 2000 nm. Genomes are linear, around 40.5kb in length. The genome has 67 open reading frames. Viral replication is cytoplasmic. Entry into the host cell is achieved by adsorption into the host cell. Dna templated transcription is the method of transcription. Archaea serve as the natural host. Transmission routes are passive diffusion. Viral Zone. ExPASy. ...

we have characterized the structure and the function of the 6.6-kda protein svtr (formerly called gp08) from the rod-shaped virus sirv1, which infects the hyperthermophilic archaeon sulfolobus islandicus that thrives at 85 degrees c in hot acidic springs. the protein forms a dimer in solution. the nmr solution structure of the protein consists of a ribbon-helix-helix (rhh) fold between residues 13 and 56 and a disordered n-terminal region (residues 1-12). the structure is very similar to that of ...

Let me tell all of u something especially the ppl who is acting very naive. Ive been in pomona since i was a kid & im now 34 years old & i know ppl from every hood in pomona (every real gang not clicks tryna be hood) i got respect from & for these ppl i know personally. Every real pomona resident knows the only hoods in pomona is 357, ws mafia, cvp, htp, p12, p13, wsp, 456, gtc, nlgc,esp, ssv, olive st, angelo mafia, oag/ocb & trg. I know real Gs from everyone of these hoods too i can name a few ppl i know personally from everyone of these gangs but im not gonna put their names up here right now, i know ppl on the streets & locked up from every gang well respected i can get a pblank in every hood no problem. Also we all know ssv was wiped out by 12st. I went to garey high & park west everybody on da south side & west was hearing the stories every weekend a member from ssv was getting ked every weekend. Remember the one who ran up & shot a car up with 4 heads from ssv in front of their house 2 ...

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Studia Universitatis Vasile Goldis, Seria Stiintele Vietii (Life Sciences Series) is indexed by: SCOPUS, EBSCO, ProQuest and Index Copernicus. This journal is a CNCSIS B+ category.

Studia Universitatis Vasile Goldis, Seria Stiintele Vietii (Life Sciences Series) is indexed by: SCOPUS, EBSCO, ProQuest and Index Copernicus. This journal is a CNCSIS B+ category.

I m looking for any info avaliable concerning maintenance of S. solfataricus. Please e-mail : dhatzini at orfeas.chemeng.ntua.gr ...

Typical growth inhibition of S. solfataricus on plates due to infectious virus. Lawns of S. solfataricus strain GΘ were prepared as in Stedman et al. (2003). T

Identification of plasmid pTC1 from Sulfolobus tengchongensis RT8-4. (a) Restriction digestion of the total DNA from S. tengchongensis RT8-4 containing pTC1. Th

Text is available under the Creative Commons Attribution-ShareAlike License; additional terms may apply. By using this site, you agree to the Terms of Use and Privacy Policy. ...

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This image shows multiple particles of a Sulfolobus spindle shaped virus (SSV) isolated from an acidic hot spring in the Kamchatka Peninsula (Russia). These spindle shaped viruses infect Sulfolobus islandicus, a hyper thermophilic microbe that belongs to the third domain of life, the Archaea. Since their discovery in the late 1970s by Carl Woese at the University of Illinois, Archaea were thought to be found only in extreme environments. In recent years, with the advent of new technologies and methodologies, we have begun to uncover that archaea are found everywhere from deep-sea hydrothermal vents to the human gut. Viruses that infect archaea have been shown to possess novel and unique characteristics not typically found in viruses that infect bacteria or eukaryotes. The viruses pictured here are one example of this. They possess a lemon-shaped morphology that has been only found in archaeal viruses. These viruses are thought to attach to cells using sticky tail fibers located at one end of ...

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