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Rabbit polyclonal CD62P antibody - N-terminal. Validated in WB, IHC and tested in Human. Immunogen corresponding to synthetic peptide.
Mouse anti Human Factor P antibody, clone 10-18 recognizes human factor P, a 53 kDa glycosylated protein present in blood serum. Factor P
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In immunolocalization experiments, we previously had shown that Cdc42p was not observed around the plasma or internal membranes and was only variably seen at the mother-bud neck region (43). The disparity between these patterns and GFP-Cdc42p targeting patterns described herein may be due to the nature of the anti-Cdc42p antibody used. The antibody was raised against a peptide sequence containing amino acids 165 to 181, a region adjacent to the membrane-targeting domain (42) and likely to be in close proximity to the plasma membrane, raising the possibility that steric hindrance interfered with efficient binding. An underestimation of Cdc42p membrane targeting may also be due to the immunolocalization protocol used, including the use of cell wall-digesting enzymes and sodium dodecyl sulfate, required for efficient Cdc42p visualization. These possibilities were supported by the observation that the GFP-Cdc42p immunolocalization pattern with anti-Cdc42p antibody was similar to that seen previously ...
TY - JOUR. T1 - Mechanisms of protein adhesion on surface films of hydrophobin. AU - Wang, Zefang. AU - Lienemann, Michael. AU - Qiau, Mingqiang. AU - Linder, Markus. PY - 2010/5/3. Y1 - 2010/5/3. N2 - Hydrophobins are adhesive proteins produced by filamentous fungi. They are in many cases secreted into the medium and adsorb readily to a number of different surfaces. They fulfill many different tasks such as the formation of various coatings and mediating adhesion of fungi to surfaces. The mechanism of how hydrophobins adhere and how they mediate fungal adhesion is of interest both from the point of view of fungal biology and for various biotechnical immobilization applications. It has been shown that hydrophobins typically form a monomolecular layer on solid substrates. We are especially interested in how a surface layer of hydrophobin can mediate the adhesion of a second layer of another protein. In this work we systematically studied how proteins adsorb onto hydrophobins that are bound as ...
We are looking for individuals or research groups to clone and express purifed proteins from fungi into industrial host. If you have such expereince please fax us your information to 1-407-743-8343 or e.mail to this address rgds MAE ...
The identification of biomarkers for Alzheimers disease is important for patient management and to assess the effectiveness of clinical intervention. Cerebrospinal fluid (CSF) biomarkers constitute a powerful tool for diagnosis and monitoring diseas
This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturers instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements ...
MacPherson S, et al. (2006) A fungal family of transcriptional regulators: the zinc cluster proteins. Microbiol Mol Biol Rev 70(3):583-604 SGD PMID 16959962 ...
During the extreme polarized growth of fungal hyphae, secretory vesicles are thought to accumulate in a subapical region called the Spitzenkörper. The human fungal pathogen Candida albicans can grow in a budding yeast or hyphal form. When it grows as hyphae, Mlc1 accumulates in a subapical spot suggestive of a Spitzenkörper-like structure, while the polarisome components Spa2 and Bud6 localize to a surface crescent. Here we show that the vesicle-associated protein Sec4 also localizes to a spot, confirming that secretory vesicles accumulate in the putative C. albicans Spitzenkörper. In contrast, exocyst components localize to a surface crescent. Using a combination of fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) experiments and cytochalasin A to disrupt actin cables, we showed that Spitzenkörper-located proteins are highly dynamic. In contrast, exocyst and polarisome components are stably located at the cell surface. It is thought that in
TY - JOUR. T1 - Saccharomyces cerevisiae proteins involved in hybrid DNA formation in vitro. AU - Heyer, W. D.. AU - Johnson, A. W.. AU - Norris, D. N.. AU - Tishkoff, D.. AU - Kolodner, R. D.. PY - 1991. Y1 - 1991. N2 - RecA-like activities that can form hybrid DNA in vitro have been identified in a wide variety of organisms. We have previously described the strand exchange protein 1 (SEP1) from the yeast Saccharomyces cerevisiae that can form hybrid DNA in vitro. Purified as an Mr 132 000 polypeptide, recent molecular and immunological studies have now shown that the native form is an Mr 175 000 polypeptide containing strand exchange activity. The gene encoding SEP1 has been cloned and sequenced. The primary sequence failed to reveal any significant sequence homology to other sequences in data base searches. In vivo SEP1 was found to be essential for normal meiosis as cells containing a homozygous insertion mutation in the SEP1 gene failed to sporulate. In order to identify additional factors ...
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TY - JOUR. T1 - Hydrophobins from Aspergillus species cannot be clearly divided into two classes. AU - Jensen,Britt Guillaume. AU - Andersen,Mikael Rørdam. AU - Pedersen,Mona Højgaard. AU - Frisvad,Jens Christian. AU - Søndergaard,Ib. PY - 2010. Y1 - 2010. N2 - Background Hydrophobins are a family of small secreted proteins with a characteristic pattern of eight cysteine residues found exclusively in filamentous fungi. They have originally been divided into two classes based on their physical properties and hydropathy patterns, and are involved in the attachment of hyphae to hydrophobic structures, the formation of aerial structures and appear to be involved in pathogenicity. Findings Analysis of nine genome sequences from seven Aspergilli revealed fifty hydrophobins, where each species displayed between two to eight hydrophobins. Twenty of the identified hydrophobins have not previously been described from these species. Apart from the cysteines, very little amino acid sequence homology was ...
Recent sequencing and assembly of the genome for the fungal pathogen Candida albicans used simple automated procedures for the identification of putative genes. We have reviewed the entire assembly, both by hand and with additional bioinformatic resources, to accurately map and describe 6,354 genes and to identify 246 genes whose original database entries contained sequencing errors (or possibly mutations) that affect their reading frame. Comparison with other fungal genomes permitted the identification of numerous fungus-specific genes that might be targeted for antifungal therapy. We also observed that, compared to other fungi, the protein-coding sequences in the C. albicans genome are especially rich in short sequence repeats. Finally, our improved annotation permitted a detailed analysis of several multigene families, and comparative genomic studies showed that C. albicans has a far greater catabolic range, encoding respiratory Complex 1, several novel oxidoreductases and ketone body degrading
A central theme in biology is to understand how different signaling outputs can be accomplished by changes to signal transduction pathways. Here, we examined epigenetic differences between two cell states in the human fungal pathogen Candida albicans. We show that cells in the "white" state are sterile due to multiple bottlenecks in MAPK signaling relative to mating-competent "opaque" cells. Alleviation of these bottlenecks by reverse engineering effectively converts sterile white cells into sexually competent cells. These results have broad implications for understanding how epigenetic changes can impact MAPK expression and signaling output, including events associated with tumorigenesis. We also propose a model for how the white-opaque switch gained control of sexual reproduction in Candida during evolution.. ...
I reveal that Saccharomyces cerevisiae Rtt109p promotes genome stability and resistance to DNA-damaging agents, and that it does this by functionally cooperating with the histone chaperone Asf1p to maintain normal chromatin structure. Furthermore, I show that, as for Asf1p, Rtt109p is required for histone H3 acetylation on lysine 56 (K56) in vivo. Moreover I show that Rtt109p directly catalyzes this modification in vitro in a manner that is stimulated by Asf1p. These data establish Rtt109p as a member of a new class of histone acetyltransferases and show that its actions are critical fro cell survival in the presence of DNA damage during S phase. In the second part of this thesis, I reveal that cells deleted for Saccharomyces cerevisiae ESC2 exhibit synthetic sickness when combined with deletions of many genes involved in maintaining genomic stability. Moreover, I show that esc2Δ mutant cells exhibit increased recombination frequency and increased relocalisation of recombination repair protein ...
To determine whether the C. albicans MTL gene cluster was required for the a1/α2-like repression activity, theGFP reporters were transformed into MTLa1deletion strains and evaluated for fluorescence. In contrast to the wild-type C. albicans strains, the MTLa1 mutant strains showed the same levels of fluorescence for all of the reporter constructs, indicating that the MTLa1 gene is required for the transcriptional repression activity (Fig. 4). Similar behavior was seen for both the complete deletion of the MTLa1 gene and for the MTLa1 homeodomain deletion, consistent with the DNA-binding domain of a1 being required for the repression activity (9). Northern (RNA) analysis also showed that transcription from the reporter constructs containing the functional hsg operators was derepressed in the MTLa1deletion mutants compared with the wild-type strain; however, in the absence of a1, the functional hsg operators still showed a slight amount of repression when compared with the mutated hsg operators ...
Aritreyee Datta*, Vikas Yadav*, ............, Kaustuv Sanyal, Ayyalusamy Ramamoorthy and Anirban Bhunia, Mode of Action of a Designed Antimicrobial Peptide: High Efficiency in Killing of the Human Fungal Pathogen Cryptococcus neoformans, Biophysical Journal 111, 1724 - 1737 (2016 ...
TY - JOUR. T1 - Subcellular distribution of glutathione and its dynamic changes under oxidative stress in the yeast Saccharomyces cerevisiae. AU - Zechmann, Bernd. AU - Liou, Liang-Chun. AU - Koffler, Barbara E.. AU - Horvat, Lucija. AU - Tomasic, Ana. AU - Fulgosi, Hrvoje. AU - Zhang, Zhaojie. PY - 2011. Y1 - 2011. U2 - 10.1111/j.1567-1364.2011.00753.x. DO - 10.1111/j.1567-1364.2011.00753.x. M3 - Article. VL - 11. SP - 631. EP - 642. JO - FEMS yeast research. JF - FEMS yeast research. SN - 1567-1356. IS - 8. ER - ...
Evolution of multigene families are considered in the review on the example of the PHO gene family encoding the structure of acid phosphatases in the yeast Saccharomyces cerevisiae. Analysis of the...
This unit presents detailed protocols for a range of centrifugation‐based subcellular fractionation procedures for the yeast Saccharomyces cerevisiae
Yeast Saccharomyces cerevisiae in vivo Prp8 splicing assay(A) Schematic representation of the two-step splicing pathway (SS, splice site; BS, branch site). Brie
TY - JOUR. T1 - Increased stress parameter synthesis in the yeast Saccharomyces cerevisiae after treatment with 4-hydroxy-2-nonenal. AU - Wonisch, Willibald. AU - Hayn, Marianne. AU - Schaur, Jörg. AU - Tatzber, Franz. AU - Kranner, Ilse. AU - Grill, Dieter. AU - Winkler, Rudolf. AU - Bilinski, Tomasz. AU - Kohlwein, Sepp-Dieter. AU - Esterbauer, Hermann. PY - 1997. Y1 - 1997. U2 - 10.1016/S0014-5793(97)00123-3. DO - 10.1016/S0014-5793(97)00123-3. M3 - Article. VL - 405. SP - 11. EP - 15. JO - FEBS letters. JF - FEBS letters. SN - 0014-5793. IS - 1. ER - ...
MOTIZUKI, M., MITSUI, K., ENDO, Y. and TSURUGI, K. (1986), Detection and partial characterization of the chromatin-associated proteases of yeast Saccharomyces cerevisiae. European Journal of Biochemistry, 158: 345-350. doi: 10.1111/j.1432-1033.1986.tb09757.x ...
Saccharomyces cerevisiae ATCC ® 201389D-5™ Designation: Genomic DNA from Saccharomyces cerevisiae Strain BY4742 (ATCC ® 201389™) Application:
Saccharomyces cerevisiae ATCC ® 201390D-5™ Designation: Genomic DNA from Saccharomyces cerevisiae Strain BY4743 (ATCC ® 201390™) Application:
Saccharomyces cerevisiae sudah sejak lama digunakan sebagai starter fermentasi pembuatan roti dan minuman beralkohol. Dalam buku ini, Saccharomyces crervisiae dimanfaatkan sebagai agensia modifikasi dalam pengolahan pangan, kemampuan S. cerevisiae dalam merombak komponen pangan, produk metabolit yang dihasilkan oleh S. cerevisiae, modifikasi terhadap perubahan sifat beberapa produk pangan oleh S. cerevisiae seperti tapioka, tempe, dan modifikasi fermentasi kakao. Pengertian dasar mengenai khamir perlu dipahami oleh mahasiswa yang khususnya mempelajari mikrobiologi pangan, mikrobiologi industri dan teknologi pangan. S.cerevisiae adalah khamir ...
Yarrowia lipolytica PEX6 protein: gene is required for peroxisome assembly in yeast Yarrowia lipolytica; protein has ATP-binding activity; contains 1025 amino acid; MW 112.258 kDa; amino acid sequence given in first source; GenBank L23858
1P-022 Saccharomyces cerevisiaeの糖代謝における転写制御ネットワークの予測(遺伝子工学,一般講演)1P-022 Saccharomyces cerevisiaeの糖代謝における転写制御ネットワークの予測(遺伝子工学,一般講演)AN10549378 ...
The Saccharomyces Cerevisiae Morphological Database(SCMD) is a collection of micrographs of budding yeast mutants. Micorgraphs of mutants with altered cell morphology were taken at Ohya Group, University of Tokyo, from a set of the haploid MATa deleted strains obtained from EUROSCARF. From the micrographs, disruptant cells are automatically extracted by our novel cell-image processing software developed at Morishita Group, University of Tokyo. Heterozygous essential gene deletion set, DAmP collection set, natural yeast strain set and others were analyzed by this software. ...
Domain architecture and assignment details (superfamily, family, region, evalue) for YLR222C from Saccharomyces cerevisiae SGD. Plus protein sequence and external database links.
Domain architecture and assignment details (superfamily, family, region, evalue) for YDR329C from Saccharomyces cerevisiae SGD. Plus protein sequence and external database links.
Saccharomyces cerevisiae Y12 - Organisms are classified by taxonomy into specified groups such as the multicellular animals, plants, and fungi; or unicellular microorganisms such as a protists, bacteria, and archaea.
Please enjoy this information on EpiCor made available through the generosity of Embrias adoption. Information on EpiCor, yeast fermentate, Saccharomyces cerevisiae.
Tetes tebu merupakan limbah pengolahan gula yang mengandung gula cukup tinggi sehingga sangat potensial dimanfaatkan sebagai media fermentasi. Fermentasi tetes tebu untuk menghasilkan bioetanol menjadi salah satu upaya megurangi jumlah limbah dan memenuhi kebutuhan Bahan Bakar Minyak (BBM) yang semakin meningkat. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pH dan lama fermentasi terhadap produksi bioetanol dari tetes tebu (molase) dengan cara fermentasi menggunakan Saccharomyces cerevisiae. Penelitian ini meliputi proses fermentasi dan pemisahan bioetanol dari media fermentasi. Proses fermentasi dilakukan dengan variasi pH 4, 4,5, dan 5, sedangkan variasi lama fermentasi dilakukan selama 3, 4, 5, dan 6 hari. Bioetanol hasil fermentasi dipisahkan dari media fermentasi dengan metode destilasi fraksinasi dan untuk mengukur kadar bioetanol digunakan metode kromatografi gas. Data yang diperoleh pada setiap perlakuan dianalisis menggunakan analisis varians (ANOVA) dan dilanjutkan ...
Gene target information for PRB1 - proteinase B (Saccharomyces cerevisiae S288C). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
Author: Stagge, F.; Genre: Thesis; Published in Print: 2010; Title: Etablierung neuartiger Fluoreszenzmarkierungen in der Bäckerhefe Saccharomyces cerevisiae für die hochauflösende Mikroskopie mitochondrialer Proteine.
Pl ss baba mikr b form ban, alkalmas mint sz rakoztat aj nd k, vagy mint tan t eszk z sz l knek s tan roknak. Az leszt gomba vagy s r leszt (Saccharomyces cerevisiae) a sarjadz - vagy leszt gomb k egy fajt ja. A korai id k ta ez a legfontosabb leszt faj - a keny rs t sn l s s rf z sn l haszn lj k. Els k nt a sz l h j n izol lt k (a s t t sz n gy m lcs k mint a...
BACKGROUND: Messenger RNA (mRNA) represents a small percentage of RNAs in a cell, with ribosomal RNA (rRNA) making up the bulk of it. To isolate mRNA from eukaryotes, typically poly-A selection is carried out. Recently, a 5´-phosphate-dependent, 5´→3´ processive exonuclease called Terminator has become available. It will digest only RNA that has a 5´-monophosphate end and therefore it is very useful to eliminate most of rRNAs in cell. RESULTS: We have found that in the pathogenic yeast Candida albicans, while 18S and 25S components isolated from yeast in robust growth phase are easily eliminated by Terminator, those isolated from cells in the nutritionally diminished stationary phase, become resistant to digestion by this enzyme ...
Yeast relevance is related to the easy determination of the link between gene and protein function (Botstein and Fink 1988). Based on the high evoluti...
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Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
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A BioProject is a collection of biological data related to a single initiative, originating from a single organization or from a consortium. A BioProject record provides users a single place to find links to the diverse data types generated for that project
Guo A (2008) CST Curation Set: 5006; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981 ...
File Title: An Improved Method for the Isolation of A^2-IPP:tRNA Isopente-nyltransferase Activity from Saccharomyces cerevisiae ...
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Article Mating and pathogenic development of the smut fungus ustilago maydis are regulated by one mitogen-activated protein kinase cascade. In the phytopathogenic fungus Ustilago maydis, pheromone-mediated cell fusion is a prerequisite for the genera...
The synthetic gene of CalBsyn was previously constructed to encode Candida antarctica lipase B (CALB). Lipase of CalBsyn gene is slightly different from...
In a lipase investigation, Candida antarctica lipase B (CALB) are explored for enzyme catalytic promiscuity. Enzyme catalytic promiscuity is shown by enzymes catalyzing alternative catalytic transformations proceeding via different transition state structures than normal. CALB normally performs hydrolysis reactions by activating and coordinating carboxylic acid/ester substrates in an oxyanion hole prior to nucleophilic attack from an active-site serine resulting in acyl enzyme formation. The idea of utilizing the carbonyl activation oxyanion hole in the active-site of CALB to catalyze promiscuous reactions arose by combining catalytic and structural knowledge about the enzyme with chemical imagination. We choose to explore conjugate addition and direct epoxidation activities in CALB by combining molecular modeling and kinetic experiments. By quantum-chemical calculations, the investigated promiscuous reactions were shown to proceed via ordered reaction mechanisms that differ from the native ping ...
Reaction rates and selectivities were measured for transacylation of fatty acid esters in solvents catalysed by Candida antarctica lipase B and by cutinase from Humicola insolens. With these enzymes classical water-based enzymology can be expanded to many different solvents allowing large variations in interaction energies between the enzymes, the substrates and the surrounding. Further ,hydrolysis reactions catalysed by Bacillus subtilis esterase 2 were investigated.. Thermodynamics analyses revealed that the enzyme contribution to reaction rate acceleration compared to acid catalysis was purely entropic. On the other hand, studies of differences in activation entropy and enthalpy between enantiomers and between homologous esters showed that high substrate specificity was favoured by enthalpic stabilisation.. Solvent was found to have a profound effect on enzyme catalysis, affecting both reaction rate and selectivity. Differences in substrate solubility will impact enzyme specificity since ...
TY - JOUR. T1 - Importance of the Candida albicans cell wall during commensalism and infection. AU - Gow, N.A.R.. AU - Hube, B.. PY - 2012/8. Y1 - 2012/8. N2 - An imbalance of the normal microbial flora, breakage of epithelial barriers or dysfunction of the immune system favour the transition of the human pathogenic yeast Candida albicans from a commensal to a pathogen. C. albicans has evolved to be adapted as a commensal on mucosal surfaces. As a commensal it has also acquired attributes, which are necessary to avoid or overcome the host defence mechanisms. The human host has also co-evolved to recognize and eliminate potential fungal invaders. Many of the fungal genes that have been the focus of this co-evolutionary process encode cell wall components. In this review, we will discuss the transition from commensalism to pathogenesis, the key players of the fungal cell surface that are important for this transition, the role of the morphology and the mechanisms of host recognition and ...
This chapter examines how cell identity influences mating-type determination, particularly in fungal pathogens. It explores cases where cell identity plays roles outside of mating type, affects cell morphology, and influences pathogenesis. The chapter begins with a description of cell type determination in the budding yeast Saccharomyces cerevisiae and uses this as a platform for exploring mechanisms in the human fungal pathogens Candida albicans and Cryptococcus neoformans as well as the plant fungal pathogen Ustilago maydis. It concludes with a short description of the influence of cell identity on the behaviors of several other plant and human fungal pathogens and how cell identity in fungi is evolving to encompass a more diverse array of fungal behaviors. A fungal pathogen in which cell identity determination has come to the fore is in the basidiomycete fungus C. neoformans. There are two obvious possibilities for specifying haploid cell identity: either the pheromone and pheromone receptor alleles
Hydrophobins are small surface active proteins that are produced by filamentous fungi. The surface activity of hydrophobin proteins leads to the formation of a film at the air-water interface and adsorption to surfaces. The formation of these hydrophobin films and coatings is important in many stages of fungal development. Furthermore, these properties make hydrophobins interesting for potential use in technical applications. The surfactant-like properties of hydrophobins from Trichoderma reesei were studied at the air-water interface, at solid surfaces, and in solution. The hydrophobin HFBI was observed to spontaneously form a cohesive film on a water drop. The film was imaged using atomic force microscopy from both sides, revealing a monomolecular film with a defined molecular structure. The use of hydrophobins as surface immobilization carriers for enzymes was studied using fusion proteins of HFBI or HFBII and an enzyme. Furthermore, sitespecifically modified variants of HFBI were shown to ...
TY - JOUR. T1 - Candida albicans ABG1 gene is involved in endocytosis. AU - Veses, Veronica. AU - Casanova, Manuel. AU - Murgui, Amelia. AU - Gow, Neil A R. AU - Martínez, José P. PY - 2009/3. Y1 - 2009/3. N2 - The human fungal pathogen Candida albicans undergoes reversible morphogenetic transitions between yeast, hyphal and pseudohyphal forms. The fungal vacuole actively participates in differentiation processes and plays a key role supporting hyphal growth. The ABG1 gene of C. albicans encodes an essential protein located in the vacuolar membranes of both yeast and hyphae. Using fluorescence microscopy of a green fluorescent protein-tagged version of Abg1p, a fraction of the protein was detected in hyphal tips, not associated with vacuolar membranes. Live cell imaging of emerging germ tubes showed that Abg1p migrated to the polarized growth site and colocalized with endocytic vesicles. Phenotypic analysis of a methionine-regulated conditional mutant confirmed that Abg1p is involved in ...
The opportunistic human pathogen Candida albicans causes both superficial and life threatening systemic infections and is a leading cause of fungal disease in immunocompromised individuals such as those with AIDS. C. albicans can grow in different cell shapes, also known as morphologies, including yeast-like cells and a variety of filamentous forms, such as true hyphae and pseudohyphae. Yeast, hyphae and pseudohyphae, have been observed at the sites of Candida infection and there is strong evidence that morphogenesis, the transition between yeast and filamentous growth forms, is essential for its virulence. Many studies have implicated the second messenger molecule cAMP in the regulation of morphogenesis due to its role in activating filamentation. Our lab and others have previously characterized the impact of the negative regulators, Nrg1, Rfg1, and Tup1 on the expression of HWP1, a hyphal specific gene. The goal of this project is to characterize whether the addition of exogenous cAMP will ...
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THE opportunistic fungal pathogen, Candida albicans, grows invasively in tissues of candidiasis patients by converting from budding yeast form cells to filamentous forms. The ability to convert from one morphology to another is important for virulence (Sobelet al. 1984; Shepherd 1985; Ryley and Ryley 1990; Lebereret al. 1997; Loet al. 1997). To understand the mechanisms by which filamentous growth is stimulated during infection, regulation of hyphal development has been studied extensively (for review, see Gow 1997). Conditions that promote hyphal growth in the laboratory include growth at elevated temperature in medium containing special components. In the absence of these conditions, growth within a matrix also promotes hyphal growth (Brownet al. 1999). The embedded condition may simulate conditions encountered by the pathogen during growth in human tissue.. Several genes whose products regulate filamentous growth have been identified (for review, see Ernst 2000), including CPH1 (Liuet al. ...
gi,7493813,pir,,T18235 transcription activator GAL11 homolog - yeast (Candida albicans) gi,3859719,emb,CAA21993.1, possible regulatory protein [Candida albicans] Length = 1145 Score = 1094 bits (2830), Expect = 0.0 Identities = 678/1145 (59%), Positives = 678/1145 (59%) Query: 1 MNIPPNQNSLQQMGGGSNPNASWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSK 60 MNIPPNQNSLQQMGGGSNPNASWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSK Sbjct: 1 MNIPPNQNSLQQMGGGSNPNASWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSK 60 Query: 61 MAQDFEKLVYERSASKEDYLRAIKMKVHQLRVQKQQIAAXXXXXXXXXXXXXXXXXXXXX 120 MAQDFEKLVYERSASKEDYLRAIKMKVHQLRVQKQQIAA Sbjct: 61 MAQDFEKLVYERSASKEDYLRAIKMKVHQLRVQKQQIAANQGGQINPQQRQQQQQQQISN 120 Query: 121 XXSMNPVNAQNVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180 SMNPVNAQNV Sbjct: 121 SNSMNPVNAQNVQFLRQQAQARSQSQAQIQARQQQLRNMVNQQSQQQQQPQPQQTVQPQS 180 Query: 181 XXXXXXXXXXXXXXXXXNVXXXXXXXXXXXXXXTQGQLPPQLVNLMRTSXXXXXXXXXXX 240 NV TQGQLPPQLVNLMRTS Sbjct: 181 QEHQQDQQNTSSQSTQQNVASGAGSGGRGNASSTQGQLPPQLVNLMRTSPIPPPLLNKMP 240 Query: ...
During the production of wine and beer, the yeast Saccharomyces cerevisiae can encounter an environment that is deficient in zinc, resulting in a sluggish or a stuck ferment. It has been shown that the Zap1p-transcription factor induces the expression of a regulon in response to zinc deficiency; however, it was evident that a separate regulon was also activated during zinc deficiency in a Zap1p-independent manner. This study discovered the Msn2p and Msn4p (Msn2/4p) transcriptional activator proteins to be an additional control mechanism inducing the stress response during zinc deficiency. Promoter sequence analysis identified the stress response element (STRE) motif, recognized by Msn2/4p, and was significantly enriched in the promoters of genes induced by zinc deficiency. An investigation using genome-wide analyses revealed a distinct regulon consisting of STREcontaining genes whose zinc-responsive expression was abolished in an msn2 msn4 double mutant. An STRE-driven lacZ reporter ...
Phosphomannosylation is a modification of cell wall proteins that occurs in some species of yeast-like organisms, including the human pathogen Candida albicans. These modified mannans confer a negative charge to the wall, which is important for the interactions with phagocytic cells of the immune systems and cationic antimicrobial peptides. In Saccharomyces cerevisiae, the synthesis of phosphomannan relies on two enzymes, the phosphomannosyltransferase Ktr6 and its positive regulator Mnn4. However, in C. albicans, at least three phosphomannosyltransferases, Mnn4, Mnt3 and Mnt5, participate in the addition of phosphomannan. In addition to MNN4, C. albicans has a MNN4-like gene family composed of seven other homologous members that have no known function. Here, using the classical mini-Ura-blaster approach and the new gene knockout CRISPR-Cas9 system for gene disruption, we generated mutants lacking single and multiple genes of the MNN4 family; and demonstrate that, although Mnn4 has a major impact on the
The Sec18 protein (Sec18p) of the yeast Saccharomyces cerevisiae has been identified as a component involved in the vesicular transport of proteins through the secretory and endocytotic pathways. Sec18p is a homologue of the mammalian protein NSF which has been shown, using a number of in vitro transport assay systems and affinity purification procedures, to interact with other proteins in a multisubunit protein complex. This work represents two approaches taken with the aim of identifying proteins that interact with Sec18p in the yeast Saccharomyces cerevisiae. Isolation of protein complexes was first attempted by affinity purification of a tagged version of Sec18p. The protein was C-terminally tagged with a protein A moiety from Staphylococcus aureus containing IgG binding domains. It was hoped that the affinity of protein A for IgG Sepharose could be used to isolate protein complexes that formed in vivo with the Sec18p. Although the fusion construct was shown to be active in vivo, specific ...
Cross talk between distinct signaling pathways regulates filamentous growth: Previous work has demonstrated that filamentous growth in yeast is regulated by at least two distinct signaling pathways, one including elements of the pheromone-responsive MAP kinase cascade and the other involving cAMP signaling (Liuet al. 1994; Kübleret al. 1997; Lorenz and Heitman 1997). Genetic evidence is consistent with a model in which the cAMP signaling branch could be activated via a Mep2p-dependent mechanism upon ammonium starvation (Lorenz and Heitman 1997, 1998). Several lines of evidence indicate that there may be some cross talk between these two pathways. First, activation of the cAMP signaling pathway, by dominant mutations in RAS2 or GPA2, or by cAMP, suppresses the morphological defects associated with MAP kinase mutant strains (Lorenz and Heitman 1997). Second, as shown here, the dominant GPA2-2 allele strongly suppresses the pseudohyphal defect of a Δste12/Δste12 mutant strain, but only weakly ...
ERK5 is a mitogen-activated protein (MAP) kinase regulated in human cells by diverse mitogens and stresses but also suspected of mediating the effects of a number of oncogenes. Its expression in the slt2Delta Saccharomyces cerevisiae mutant rescued several of the phenotypes caused by the lack of Slt2p (Mpk1p) cell integrity MAP kinase. ERK5 is able to provide this cell integrity MAP kinase function in yeast, as it is activated by the cell integrity signaling cascade that normally activates Slt2p and, in its active form, able to stimulate at least one key Slt2p target (Rlm1p, the major transcriptional regulator of cell wall genes). In vitro ERK5 kinase activity was abolished by Hsp90 inhibition. ERK5 activity in vivo was also lost in a strain that expresses a mutant Hsp90 chaperone. Therefore, human ERK5 expressed in yeast is an Hsp90 client, despite the widely held belief that the protein kinases of the MAP kinase class are non-Hsp90-dependent activities. Two-hybrid and protein binding studies ...
Autophagy is an intracellular process responsible for the degradation and recycling of cytoplasmic components. It selectively removes harmful cellular material and enables the cell to survive starvation by mobilizing nutrients via the bulk degradation of cytoplasmic components. While research over the last decades has led to the discovery of the key factors involved in autophagy, the pathway is not yet completely understood. The first studies of autophagy on a molecular level were conducted in the yeast Saccharomyces cerevisiae. Building up on these studies, many homologs have been found in higher eukaryotes. Yeast remains a highly relevant model organism for studying autophagy, with a wide range of established methods to elucidate the molecular details of the autophagy pathway. In this review, we provide an overview of methods to study both selective and bulk autophagy, including intermediate steps in the yeast Saccharomyces cerevisiae. We compare different assays, discuss their advantages and
Saccharomyces Cerevisiae Yeast Cells Sem Scanning as a 8x6 Glass Mount from CMSP Photo Prints. Fast and safe delivery. Saccharomyces Cerevisiae Yeast Cells. these Microorganisms Fungi are Used to Raise Bread Dough the Yeasts Produce
Yeast cells. Coloured Scanning Electron Micrograph (SEM) of yeast cells, Saccharomyces cerevisiae. This fungus, also known as Bakers or Brewers yeast, consists of single vegetative cells. Some cells can be seen dividing by budding off new cells. Saccharomyces cerevisiae ferments sugar, producing alcohol and carbon dioxide in the process. It has long been used in brewing beer, the production of wine and in baking leavened bread (carbon dioxide causes the dough to rise). Medically, dried Bakers yeast is used as a rich source of vitamin B1, riboflavin and nicotinic acid. Magnification: x125 at 6x7cm size. x200 at 4x5 - Stock Image B250/0646
Read "The Genetic Control of Cell Growth and Development in Yeast Saccharomyces cerevisiae: Disturbed Sporulation in Diploids with a Decreased Activity of the Ras/cAMP Signal Transduction Pathway, Russian Journal of Genetics" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Under amino acid starvation conditions, the bakers" yeast Saccharomyces cerevisiae activates a system called "General control of amino acid biosynthesis". Gcn4p, the transcription factor of this system induces the expression of more than 50 genes involved in the different amino acid biosynthetic pathways. In this thesis it could be shown that during simultaneous limitation of amino acids and nitrogen the general control is not activated. More exactly, even a decrease of the Gcn4p activity was detected, which was traced back onto a reduction of the Gcn4 protein amount in the cell. This decrease of the intracellular concentration was caused by translational control of the GCN4 mRNA, which was able to repress even a 2-fold increase of the GCN4 transcription rate. Furthermore during nitrogen starvation conditions no correlation between the stature of eIF-2 phosphorylation and GCN4 expression was observed. For this reason an involvement of the already known mechanism of translation! al regulation of ...
Effect of the msb3msb4 double mutation on the intracellular pool of purine nucleotides in the yeast Saccharomyces cerevisiae: application to the study of the biological activity of the oncogenic human protein oncTre210p ...
Hinweis nicht immer die sd [archiv] autoimmune. (gnetz/gesundheit_az/index_ad/darmpilz/darmpilz oder auch chronische krankheiten hat der pilz leichtes spiel. Candida albicans ist nicht. Zentrum der gesundheit candida albicans can yeast. Zentrum der gesundheit candida albicans • end up an iherb rewards member and earn unlimited rewards by sharing your preferred iherb merchandise with others. Candidainfektion natürlich bekämpfen. […]. Continue reading ...
Getting Better Intestinal Health through the Addition of Yeast (Saccharomyces Cerevisiae) Combined with Threonine in Broilers Diets
I use this paper in my graduate genetics course. It describes a global screen for synthetic defects involving DNA integrity, which reveals a network of 16 functional modules. The paper illustrates screens based on genetic interactions (in this case, synthetic lethality or fitness defects) and the systems biology used to evaluate the results of such a screen. It also illustrates the use of Saccharomyces cerevisiae as a model system ...
Winters MJ, Pryciak PM. Interaction with the SH3 domain protein Bem1 regulates signaling by the Saccharomyces cerevisiae p21-activated kinase Ste20. Mol Cell Biol. 2005 Mar; 25(6):2177-90 ...
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Saccharomyces cerevisiae is a species of yeast. It is perhaps the most useful yeast, having been instrumental to baking and brewing since ancient times. It is believed that it was originally isolated FROM chado.the skins of grapes (one can see the yeast as a component of the thin white film on the skins of some dark-colored fruits such as plums; it exists among the waxes of the cuticle). It is one of the most intensively studied eukaryotic model organisms in molecular and cell biology, much like Escherichia coli as the model bacterium. It is the microorganism behind the most common type of fermentation. S. cerevisiae cells are round to ovoid, 5-10 micrometres in diameter. It reproduces by a division process known as budding ...
The biological interpretation of genetic interactions is a major challenge. Recently, Kelley and Ideker proposed a method to analyze together genetic and physical networks, which explains many of the known genetic interactions as linking different pathways in the physical network. Here, we extend this method and devise novel analytic tools for interpreting genetic interactions in a physical context. Applying these tools on a large-scale Saccharomyces cerevisiae data set, our analysis reveals 140 between-pathway models that explain 3765 genetic interactions, roughly doubling those that were previously explained. Model genes tend to have short mRNA half-lives and many phosphorylation sites, suggesting that their stringent regulation is linked to pathway redundancy. We also identify pivot proteins that have many physical interactions with both pathways in our models, and show that pivots tend to be essential and highly conserved. Our analysis of models and pivots sheds light on the organization of the
Saccharomyces cerevisiae, HA12, a, ade1 ade2. Our materials are for use in the experiments developed by the yeast genetics educational network (GENE project) created by Dr. Tom Manney at Kansas State University (KSU). These experiments are great for hands-on teaching of some of the basic concepts in...
Domain architectures containing the following SCOP superfamilies _gap_,81296,_gap_ in Saccharomyces cerevisiae M22. Domain architectures illustrate each occurrence of _gap_,81296,_gap_.
Domain architectures containing the following SCOP superfamilies _gap_,81296,_gap_ in Saccharomyces cerevisiae LalvinQA23. Domain architectures illustrate each occurrence of _gap_,81296,_gap_.
BioAssay record AID 460553 submitted by ChEMBL: Antiaging effect in Saccharomyces cerevisiae K6001 expressing uth1 mutant assessed as extension of replicative life span after 2 days.
A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc15, a protein kinase involves in cytokinesis. These phase and GFPimages ...
Saccharomyces cerevisiae is a species of yeast. It is believed to be isolated from the skin of grapes. It is one of the most intensively studied eukaryot..
A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged TEM1. TEM1 is a GTP-binding protein of the ras superfamily involved in te...
TY - JOUR. T1 - Sugar uptake and subsequent ester on higher alcohol production by Saccharomyces cerevisiae. AU - Stewart, Graham George. AU - Younis, O S. PY - 1998. Y1 - 1998. M3 - Article. VL - 104. SP - 255. EP - 264. JO - Journal of the Institute of Brewing. JF - Journal of the Institute of Brewing. SN - 0046-9750. ER - ...
The optimum pH range of saccharomyces cerevisiae is typically between four and six in the pH scale, as claimed by a 2005 study conducted by Neelakantam V. Narendranath and Ronan Power, which was...
Functional Overlap between eIF4G Isoforms in Saccharomyces cerevisiae. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Du, N., Stillman, B. (2001) Identification and characterization of ORC-interacting protein: Yph1p in Saccharomyces cerevisiae. Clinical Cancer Research, 7 (11). 3793S-3793S. ISSN 1078-0432 ...
Lacadena, Javier y Álvarez García, Elisa y Carreras Sangrà, Nelson y Herrero Galán, Elías y Alegre Cebollada, Jorge y García Ortega, Lucía y Oñaderra, Mercedes y Gavilanes, José G. y Martínez del Pozo, Álvaro (2007) Fungal ribotoxins: molecular dissection of a familyof natural killers. FEMS Microbiology Reviews, 31 . pp. 212-237. ISSN 0168-6445 ...
Conidiation in the filamentous ascomycete Aspergillus nidulans requires activation of brlA, a well-characterized transcriptional regulator of genes that are induced specifically during asexual develop
Complete information for GAPDH gene (Protein Coding), Glyceraldehyde-3-Phosphate Dehydrogenase, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Localization of Chs2-YFP in yeast and hyphal cells grown in the presence of caspofungin and CaCl2 and CFW. A-F. Yeast cells of the CHS2-YFP/chs2Δ0 strain wer
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Before scientist learned how to make a synthetic growth hormone, removing it painstakingly in small amounts from the pituitary glands of human cadavers. a. scientists ...