Leukocyte adhesion deficiency/congenital disorder of glycosylation IIc (LAD II/CDG IIc) is a genetic disease characterized by a decreased expression of fucose in glycoconjugates, resulting in leukocyte adhesion deficiency and severe morphological and neurological abnormalities. The biochemical defect is a reduced transport of guanosine diphosphate-L-fucose (GDP-L-fucose) from cytosol into the Golgi compartment, which reduces its availability as substrate for fucosyltransferases. The aim of this study was to determine the effects of a limited supply of GDP-L-fucose inside the Golgi on core fucosylation (a1,6-fucose linked to core N-acetylglucosamine [GlcNAc]) of N-linked glycans in LAD II fibroblasts. The results showed that, although [3H]fucose incorporation was generally reduced in LAD II cells, core fucosylation was affected to a greater extent compared with other types of fucosylation of N-linked oligosaccharides. In particular, core fucosylation was found to be nearly absent in biantennary ...

Looking for online definition of 2-amino-2-deoxy-d-galactose in the Medical Dictionary? 2-amino-2-deoxy-d-galactose explanation free. What is 2-amino-2-deoxy-d-galactose? Meaning of 2-amino-2-deoxy-d-galactose medical term. What does 2-amino-2-deoxy-d-galactose mean?

Glycosylation drives critical processes important for mammalian cell-cell and cell-matrix interactions. Alpha-l-fucose (α-l-f) is a key monosaccharide component of oligosaccharides that has been found to be overexpressed during tumor progression. Modification of cell surface fucosylation, we hypothesized, alters tumor cell phenotype and function at the end of the neoplastic progression cascade including tumor invasion. Alpha-l-fucosidase (α-l-fase) is a glycosidase that specifically removes (α-l-f) from oligosaccharide sites. We first verified the effectiveness of the α-l-fase to specifically decrease the level of α-l-f on the cell surface of several human breast cancer cell lines and also examined the recovery time for these cells to repopulate their surfaces. To investigate the potential effect of defucosylation on tumor functions, we studied the proliferation, and invasion in vitro of human breast cancer MDA-MB-231 cells as the representative cell model. We further examined several ...

Mucins are high-molecular weight glycoproteins (0.25-20 MDa) containing one or more domains that are heavily O-glycosylated. Their implications as targets for cancer treatment have increased the interest in these glycoproteins, mainly in the fields of vaccines and antibodies. However, mucins present high heterogeneity, posing challenges that affect purification processes and quality control analysis. In that sense, it is necessary to develop and improve downstream processes and analytical methods to characterize these products. Here a tool based on biolayer interferometry analysis to improve mucins detection and quantification in a fast, simple and label free-way is presented. Taking advantage of lectin recognition of mucins carbohydrate structures, several lectins were evaluated and immobilized on streptavidin biosensors. Different assay conditions were optimized and the most suitable lectin, Aleuria aurantia lectin (AAL), was selected. Bovine Submaxillary Gland and human MUC5B mucins were ...

Définitions de 1 3 alpha l fucosidase, synonymes, antonymes, dérivés de 1 3 alpha l fucosidase, dictionnaire analogique de 1 3 alpha l fucosidase (anglais)

Structural and functional evidence indicate that the fucose-binding lectin present on bull sperm is a seminal plasma protein secreted by the seminal vesicles called PDC-109 (BSP-A1/A2) (Gwathmey et al 2003, Ignotz et al 2001). Upon ejaculation, this protein associates with the sperms plasmatic membrane enabling it to bind to the oviductal epithelium (figure 1). This proposed mechanism explains the limited binding capacity of sperm that have not been in contact with seminal fluids i.e., epididimal sperm (Gwathmey et al 2003, Petrunkina et al 2001). Furthermore, excess PDC-109 in seminal plasma may block sperm binding sites on posterior portions of the female reproductive tract facilitating sperm transport to the oviducts (Suárez, 2002). Mucus secretions. Overstreet and Cooper (1975) first observed that rabbit sperm collected from the isthmus of does before ovulation were immotile or weakly motile. This observation is interpreted as a transitory inhibition or suppression of sperm flagellar ...

Electrospray mass spectrometry (ESI-MS) was used to measure the masses of an intact dimeric monoclonal antibody (Mab) and assess the fucosylation level. The Mab under study was EG2-hFc, a chimeric human-camelid antibody of about 80 kDa (A. Bell et al., Cancer Lett., 2010, 289(1), 81-90). It was obtained from Glycomics & Glycoproteomics: From Analytics to Function

There is a clinical need for methods that can quantify regional hepatic function non-invasively in patients with cirrhosis. Here we validate the use of 2-[(18)F]fluoro-2-deoxy-d-galactose (FDGal) PET/CT for measuring regional metabolic function to this purpose, and apply the method to test the hypothesis of increased intrahepatic metabolic heterogeneity in cirrhosis ...

This is a single-center, randomized, two-arm, study evaluating the safety, feasibility and immunogenicity of neoadjuvant degarelix(Arm A) or BMS-986218 plus degarelix (Arm B) prior to radical prostatectomy in men with high-risk localized prostate cancer (neo-RED-P). Our primary objective is to characterize safety, tolerability, and feasibility of degarelix with or without BMS-986218 in the neoadjuvant setting. The trial will monitor toxicity and safety, as well as surgery related adverse events. The secondary objectives will be to evaluate an immune response consistent with the proposed mechanism of action of BMS-986218, depletion of Tregs, and to assess the pathologic complete response rate, PSA response rate and time-to-PSA recurrence following treatment ...

[button size=small text=MSDS & Datasheet link=/wp-content/uploads/media/BCDatasheets_C_10.26/GCP/CCG/CCG-0004-10.pdf]Fucose BSA Colloidal Gold Conjugate, 1mL

In gene-targeted mouse models for cystic fibrosis (CF), the disease is mainly manifested by mucus obstruction in the intestine. To explore the mucus composition, mucins insoluble and soluble in 6 M guanidinium chloride were purified by three rounds of isopycnic ultracentrifugation from the small and large intestines of CF mice (Cftr(m1UNC)/Cftr(m1UNC)) and compared with wild-type mice. The amino acid composition was typical of that for mucins and showed increased amounts of the insoluble (2.5-fold increase) and soluble (7-fold increase) mucins in the small intestine of the CF mice compared with wild-type mice. Mucins from the large intestine of both wild-type and CF mice showed a high but constant level of fucosylation. In contrast, the insoluble and soluble mucins of the small intestine in CF mice revealed a large increase in fucose, whereas those of wild-type mice contained only small amounts of fucose. This increased fucosylation was analysed by releasing the O-linked oligosaccharides ...

Fucose galactose carbohydrates have been shown to induce neuronal outgrowth. The invention includes methods of inducing neuronal outgrowth using carbohydrates, assemblies, and polymers bearing fucose-

1OD3: Structure and Ligand Binding of Carbohydrate-Binding Module Cscbm6-3 Reveals Similarities with Fucose-Specific Lectins and Galactose-Binding Domains

Shop L-fucose mutarotase ELISA Kit, Recombinant Protein and L-fucose mutarotase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.

Host fucosylation increases tolerance of a pathogena, Difference in % weight loss between LPS-injected C. rodentium-infected and uninfected mice (mean±s.e.m.;

PROCESS FOR PRODUCING AN OXIDIZED NANOFIBRILLAR CELLULOSE HYDROGEL | METHOD AND APPARATUS FOR CONTROLLING THE CATALYTIC OXIDATION OF CELLULOSE | MULTISPECIFIC ANTIBODIES | OPTIMIZED CROSS-SPECIES SPECIFIC BISPECIFIC SINGLE CHAIN ANTIBODY CONTRUCTS | C-MET ANTIBODY COMBINATIONS |

The Golm Metabolome Database (GMD) facilitates the search for and dissemination of mass spectra from biologically active metabolites quantified using GC-MS.

The Golm Metabolome Database (GMD) facilitates the search for and dissemination of mass spectra from biologically active metabolites quantified using GC-MS.

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J:185112 Park D, Choi D, Lee J, Lim DS, Park C, Male-like sexual behavior of female mouse lacking fucose mutarotase. BMC Genet. 2010;11:62 ...

TY - JOUR. T1 - O-fucosylation of muscle agrin determines its ability to cluster acetylcholine receptors. AU - Kim, Mi Lyang. AU - Chandrasekharan, Kumaran. AU - Glass, Matthew. AU - Shi, Shaolin. AU - Stahl, Mark C.. AU - Kaspar, Brian. AU - Stanley, Pamela. AU - Martin, Paul T.. PY - 2008/10/29. Y1 - 2008/10/29. N2 - Protein O-fucosyltransferase 1 (Pofut1) transfers fucose to serine or threonine on proteins, including Notch receptors, that contain EGF repeats with a particular consensus sequence. Here we demonstrate that agrin is O-fucosylated in a Pofut1-dependent manner, and that this glycosylation can regulate agrin function. Fucosylation of recombinant C45 agrin, both active (neural, z8) and inactive (muscle, z0) splice forms, was eliminated when agrin was overexpressed in Pofut1-deficient cells or by mutation of a consensus site for Pofut1 fucosylation (serine 1726 in the EGF4 domain). Loss of O-fucosylation caused a gain of function for muscle agrin such that it stimulated AChR ...

Lectins, a group of proteins that bind to cell surface carbohydrates and play important roles in innate immunity, are widely used experimentally to distinguish cell types and to induce cell proliferation. Eel serum lectins have been useful as anti-H hemagglutinins and also in lectin histochemistry as fucose-binding lectins (fucolectins), but their structures have not been determined. Here we report the primary structures and the sites of synthesis of eel fucolectins. Eel serum fucolectins were separated by two-dimensional gel electrophoresis and sequenced. cDNA cloning, based on the amino acid sequence information, and Northern blot analysis indicated that 1) the fucose-binding lectins are secretory proteins and have unique structures among the lectins, exhibiting only weak similarities to frog pentraxin, horseshoe crab tachylectin-4, and fly fw protein; 2) there are at least seven closely related members; and 3) their messages are abundantly expressed in the liver and in significant levels in ...

Introduction: The aim of this study was to compare fucose and sialic acid residue expression on fibronectin and alpha(1)-acid glycoprotein in the. seminal plasma PF-6463922 solubility dmso of men suspected of infertility and suffering from leukocytospermia.. Subjects and methods: Seminal ejaculates were collected from 27 leukocytospermic and 18 healthy, normozoospermic men. The relative degree of fucosylation and sialylation of fibronectin and alpha(1)-acid glycoprotein was estimated by ELISA using fucose and sialic acid specific lectins from Aleuria aurantia, Lotus tetragonolobus, and Ulex europaeus as well as Maackia amurensis and Sambucus nigra, respectively.. Results: Leukocytospermic seminal fibronectin, in comparison with fibronectin of normal fertile group, showed lower relative reactivity with AAL, LTA and UEA, and higher reactivity with MAA and SNA, while the AGP of the leukocytospermic group was less reactive with AAL, and the relative reactivity. buy 5-Fluoracil with LTA and MAA was ...

This gene is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the MFNG (GeneID: 4242) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. [provided by RefSeq, May 2018 ...

Notch is a transmembrane receptor for the transmembrane ligands Delta and Jagged (also known as Serrate). Signaling by Notch is important for establishing boundaries during development (see accompanying article by Fortini). Fringe modifies Notch signaling when expressed in Notch-expressing cells. Two groups, Brückner et al. and Moloney et al., show that Fringe is a glycosyltransferase that catalyzes the elongation of O-linked fucose residues on the epidermal growth factor repeats of the Notch receptor. Brückner et al. show that coexpression of Fringe and Notch enhances the interaction between Notch and Delta and that the glycosyltransferase activity of Fringe is essential for this activity. Moloney et al. show that when Notch is modified by Fringe, activation of Notch by Jagged1 is inhibited. Thus, carbohydrate modification of the Notch receptor fine-tunes the cellular responsiveness to Notch ligands, allowing the establishment of discrete boundaries of Notch signaling. Carbohydrate ...

Breast-fed infants have Bifidobacterium-rich gut microbiota compared to infants fed formula. Fucosylated oligosaccharides are the major components of human

Direct mass spectrometric analysis of aberrant protein glycosylation is a challenge to the current analytical techniques. Except lectin affinity chromatography, no other glycosylation enrichment techniques are available for analysis of aberrant glycosylation. In this study, we developed a combined chemical and enzymatic strategy as an alternative for the mass spectrometric analysis of aberrant glycosylation. Sialylated glycopeptides were enriched with reverse glycoblotting, cleaved by endoglycosidase F3 and analyzed by mass spectrometry with both neutral loss triggered MS3 in collision induced dissociation (CID) and electron transfer dissociation (ETD). Interestingly, a great part of resulted glycopeptides were found with fucose attached to the N-acetylglucosamine (N-GlcNAc), which indicated that the aberrant glycosylaton that is carrying both terminal sialylation and core fucosylation was identified. Totally, 69 aberrant N-glycosylation sites were identified in sera samples from hepatocellular ...

Exhibits fucose binding activity and racemase and epimerase activity, acting on carbohydrates and derivatives. Involved in several processes, including female mating behavior; fucose metabolic process; and negative regulation of neuron differentiation. Orthologous to human FUOM (fucose mutarotase ...

The goal of this clinical research study is to learn if it is safe and feasible to transplant changed cord blood for patients with leukemia or lymphoma. Researchers also want to learn if this can help to control the disease.. The cord blood will be changed to make use of sugar that is found in small amounts in blood cells. It plays a role in signaling where in the body the transplanted cells should go to. Adding more sugars to the cord blood cells in the laboratory is designed to help the cord blood cells find their way faster to the bone marrow. This may help your blood counts to recover faster. This process is called fucosylation.. Anti-thymocyte globulin (ATG) is a protein that removes immune cells that cause damage to the body.. Clofarabine is designed to interfere with the growth and development of cancer cells.. Fludarabine is designed to interfere with the DNA (genetic material) of cancer cells, which may cause the cancer cells to die. This chemotherapy is also designed to block your ...

Mannose can randomize to glucose, galactose, and fucose. However, because the label in [3H]2-mannose is on the hydrogen of C-atom 2, the label would be predominantly found in mannose and fucose. In rare instances, the label from [3H]2-mannose can be found in glucose and lactic acid, suggesting that during oxidation and reduction reaction resulting in the epimerization of mannose to glucose, the label may be transferred from C-atom 2 to C-atom 1 (see Ref. 31). Thus, the major labeled spot in Fig. 1⇑ could be due to a glycan modifying a lipid and/or a protein. For three reasons we believe that the [3H]2-mannose-labeled compound is a glycolipid and not a glycopeptide or glycoprotein. First, the control Db-sol, also a glycoprotein with two N-linked glycans, processed in the same manner as sCD1d1, does not contain the tritiated spots seen with the CD1 sample (Fig. 1⇑). Second, the CD1d1-associated [3H]2-mannose-labeled ligands are PI-PLC sensitive as described below. Third, sialic acid-containing ...

Modulation of protein function by covalent modification is of increasing importance as a factor in disease and as a therapeutic target. The discovery of new protein modifications and their function remains a challenge. Using protein/peptide purification and mass spectrometry as well as molecular and cell biology techniques, we focus on two unique forms of glycosylation in secreted proteins, C-mannosylation of tryptophan and O-fucosylation of serine or threonine residues. About 50 examples of the former modification are known, in organisms ranging from C. elegans to man. We are studying enzymes involved in the process and analysing specific proteins lacking the modification. Of the two kinds of O-fucose-linked polysaccharides known, O-linked tetrasaccharide appears to function in intercellular signalling. We have found the first protein containing the product of the second pathway, i.e. Glc-Fuc-O-Ser/Thr and are seeking its function ...

Substantial evidence suggests that cell surface carbohydrate antigens, particularly those containing fucose residues, are related to cancer malignancy. To investigate the mechanisms underlying cell...

T-cells infiltrating sites of inflammation of the skin typically express the cutaneous lymphocyte-associate antigen (CLA). This antigen is defined by the binding of the monoclonalCLA antibody HECA-452. The CLA antigen is a fucose-containing oligosaccharide and is found on many of the ligands that are recognized by the adhesion proteins P-selectin and E-selectin.

1962 m. Antanas Buračas gavo paskyrimą dirbti KPI Politinės ekonomijos katedroje asistento pareigose, po pusmečio paskirtas vyr dėstytoju. Pragyventi iš dėstytojo algos su šeima be mokslo laipsnio buvo sunku, teko papildomai dirbti ir Lietuvos žemės ūkio akademijoje. Todėl 1964-1967 m. įstojo į TSRS Mokslų akademijos Pasaulinės ekonomikos ir tarptautinių santykių instituto aspirantūrą (vadovė I. Osadčaja). Jau pirmaisiais mokslo metais A. Buračas visasąjunginiame suvažiavime padarė daug dėmesio sulaukusį pranešimą iš Vakarų ekonominių doktrinų metodologijos. Tai vėliau jam atvėrė duris į centrinius TSRS MA socialinių mokslų institutus ir labai padėjo tiek publikuotis visasąjunginėse leidyklose Maskvoje ir Novosibirske, tiek ir pagreitintai parengti habilitacijos darbą. Jau kandidatinės disertacijos iš gyventojų išlaidų teorijų gynimo metu 1967 m. gegužės mėn. abu oponentai gynimo taryboje siūlė išimties tvarka Antanui Buračui suteikti ...

A major focus in the field of glycomics has been the development of new strategies for the detection and quantification of glycans and glycoconjugates. With alterations in glycoconjugate structure being a hallmark of various cancers, these strategies can discover new cancer biomarkers and be developed into new clin. diagnostic tools. Here we report chemoenzymic strategies for the rapid, sensitive detection of cancer-relevant fucosylated glycoconjugates. Our methods exploit non-mammalian glycosyltransferases that accept non-natural donor substrates. We then use Click chem. to append reporter tags for the detection of these glycans. We have developed methods for the detection of glycans contg. fucoseα(1-2)galactose (Fucα(1-2) Gal), a motif implicated cancer pathogenesis, as well as core fucosylated glycans, a carbohydrate modification that is upregulated in various cancer states and mediates cell signaling events. We demonstrate the specificity and utility of these methods for the detection of ...

The enzyme propanediol oxidoreductase, which converts the lactaldehyde formed in the metabolism of fucose and rhamnose into propane-1,2-diol under anaerobic conditions, was investigated in Escherichia coli, Klebsiella pneumoniae and Salmonella typhimurium. Structural analysis indicated that the enzymes of E. coli and K. pneumoniae have the same Mr and pI, whereas that of Salm. typhimurium also has the same Mr but a slightly different pI. One-dimensional peptide mapping showed identity between the E. coli and K. pneumoniae enzymes when digested with α-chymotrypsin, Staphylococcus aureus V8 proteinase or subtilisin. In the case of Salm. typhimurium, this held only for the subtilisin-digested enzymes, indicating that the hydrophobic regions were preserved to a considerable extent. Anaerobically, the three species induced an active propanediol oxidoreductase when grown on fucose or rhamnose. An inactive propanediol oxidoreductase was induced in Salm. typhimurium by either fucose or rhamnose under ...

Background/Purpose: Systemic sclerosis (SSc) is a connective tissue disease characterized by systemic fibrosis. The dysregulation of transforming growth factor-β (TGF-β) signaling causes proliferation of myofibroblasts and results in the uncontrolled release of extracellular matrix. We have published that fucosyltransferase-1 (Fut1), an α-1,2 fucosyltransferase, plays an important role in rheumatoid arthritis synovial fibroblast proliferation. In this study, we examine the role of Fut1 in TGF-β receptor (TGF-βR) fucosylation, downstream signaling pathways, and target genes involved in scleroderma pathogenesis. Methods: qPCR and ELISA were performed to assess the levels of Fut1 in SSc dermal fibroblasts and patient sera, respectively. To determine fucosylation of TGF-βR1, TGF-βR1 was immunoprecipitated from wild type (WT) dermal fibroblasts and immunoblotted with ulex europaeus agglutinin 1, which detects α-1,2 fucosylated protein. To confirm TGF-bR1 fucosylation, Fut1 was knocked down in ...

Marine environments have a high quantity and diversity of sulfated polysaccharides. In coastal regions brown algae are the most abundant biomass producers and their cell walls have fucose-containing sulfated polysaccharides (FCSP), known as fucans and/or fucoidans. These sulfated compounds have been widely researched for their biomedical properties, namely the immunomodulatory, haemostasis, pathogen inhibition, anti-inflammatory capacity, and antitumoral. These activities are probably due to their ability to mimic the carbohydrate moieties of mammalian glycosaminoglycans. Therefore, the FCSP are interesting compounds for application in health-related subjects, mainly for developing scaffolds for delivery systems or tissue regeneration. FCSP showed potential for these applications also due to their ability to form stable 3D structures with other polymers able to entrap therapeutic agents or cell and growth factors, besides their biocompatibility and biodegradability. However, for the clinical use of

Affinity purified Lotus tetragonolobus lectin (LTL) is isolated from lotus seeds and is composed of 240 amino acid residues. LTL is a glycoprotein consists of four subunits. This lectin has different isoelectric points of pH 7.3, 7.6, 7.9, 8.2 and a carbohydrate specificity toward α-fucose, it is most inhibited by L-fu

When in 1981 Rob Aalberse from the University of Amsterdam noticed the enormous cross-reactivity of some patients´ sera against virtually any plant and even insects, notably, insect venoms,[1] it took ten years to arrive at a possible structural explanation of this phenomenon. 1991, Japanese researchers determined the structure of the epitope common to horseradish peroxidase and Drosophila neurons as being an asparagine-linked oligosaccharide (N-glycan) containing a xylose and a core-linked α1,3-linked fucose residue.[2] These structural features are not present in humans and animals. Core α1,3-fucose was then found to be relevant for the binding of patients´ IgE to honeybee venom allergens,[3] which contain N-glycans with structural similarities to plant N-glycans. Ever since then, core α1,3-fucose emerged as the structural element most relevant as a CCD in plants and insect allergens. Much later, both xylose and core α1,3-fucose were revealed as heart pieces of two independent glycan ...

The use of marine-origin polysaccharides has increased in recent research because they are abundant, cheap, biocompatible, and biodegradable. IACS-9571 PropertiesFucoidan was isolated for the first time in 1913 and refers to a family of sulfated polysaccharides isolated from several brown algae and marine invertebrates [29]. The structure shown in Physique 3 presents a substantial quantity of L-fucose and sulfate ester groups, but according to the source, fucoidan can exhibit different structures [30,31]. Open up in another window Body 3 Chemical framework of fucoidan device from may be the most common algae types utilized to extract the easiest polymer of the Rabbit polyclonal to AKAP13 complete group having just L-fucose and sulfate products [32,33]. Structurally, fucoidan includes a backbone of -(1C3)-connected fucose IACS-9571 products or it really is composed of duplicating disaccharide products of -(1C3)- and -(1C4)-connected fucose residues with O-2 arm. With regards to the framework of ...

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DC-SIGN recognizes both internal branched mannose residues as well as terminal di-mannoses, α1-3 and α1-4 fucosylated glycan structures and certain N-aceltylglucosamine containing molecules on self proteins and/or pathogens ,ref name=Feinberg 2001/,,ref name=Guo 2004,Guo Y, Feinberg H, Conroy E, Mitchell DA, Alvarez R, Blixt O, Taylor ME, Weis WI and Drickamer K. 2004. Structural basis for distinct ligand-binding and targeting properties of the receptors DC-SIGN and DC-SIGNR. Nat Struct Mol Biol. 11:591-598,/ref,,ref,Mitchell DA, Fadden AJ and Drickamer K. 2001. A novel mechanism of carbohydrate recognition by the C-type lectins DC-SIGN and DC-SIGNR. Subunit organization and binding to multivalent ligands. J Biol Chem. 276:28939-28945,/ref,,ref name=Liempt 2006,van Liempt E, Bank CM, Mehta P, Garcia-Vallejo JJ, Kawar ZS, Geyer R, Alvarez RA, Cummings RD, Kooyk Y and van Die I. 2006. Specificity of DC-SIGN for mannose- and fucose-containing glycans. FEBS Lett. 580:6123-6131,/ref,,br,,br ...

Alpha-l-fucosidase (AFU) plays vital roles in some physiological processes that closely relate to several diseases. Herein, a ratiometric fluorescent probe for the alpha-l-fucosidase (AFU) assay based on a single fluorophore has been developed by functionalizing a new fluorophore platform (6-bromo-anthracene MCF Editors Recommendation

Streptomyces venezuelae decarboxylase JadN (jadN), JadX (jadX), NDP-hexose2,3-dehydratase (jadO), NDP-hexose 3-ketoreductase (jadP), NDP-glucosephosphate nucleotidyltransferase (jadQ), glycosyltransferase (jadS),NDP-hexose 4,6-dehydratase (jadT), NDP-4-keto-6-deoxyhexose 5-epimerase(jadU), NDP-4-keto-6-deoxyhexose 4-ketoreductase (jadV), JadR* (jadR*), andJadY (jadY) genes, complete cds; and JadZ (jadZ) gene, partial ...

Relationship between Antioxidants Glutathione and Total ?-L- Fucose as Tumor Markers in Breast Cancer Patients. Mufeed J.Ewadh,Nadia Hassan Kadhum,Kadhium J.Al Hamdani, Alaa S.Alawad ...

D-mannose on naturaalne suhkur, mis leidub teatud taimedes ja jõhvikas ning mis seob endaga patogeensed bakterid nagu E.coli ja teised, mis tekitavad põiepõletiku. D-mannose ja jõhvika ekstrakti toimel bakterid ei kleepu põie seinale ning nende paljune...

TY - JOUR. T1 - Fucose ameliorates tryptophan metabolism and behavioral abnormalities in a mouse model of chronic colitis. AU - Borisova, Mariya A.. AU - Snytnikova, Olga A.. AU - Litvinova, Ekaterina A.. AU - Achasova, Kseniya M.. AU - Babochkina, Tatiana I.. AU - Pindyurin, Alexey V.. AU - Tsentalovich, Yuri P.. AU - Kozhevnikova, Elena N.. N1 - Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 2020/2/11. Y1 - 2020/2/11. N2 - Growing evidence suggests that intestinal mucosa homeostasis impacts immunity, metabolism, the Central Nervous System (CNS), and behavior. Here, we investigated the effect of the monosaccharide fucose on inflammation, metabolism, intestinal microbiota, and social behavior in the Dextran Sulfate Sodium (DSS)-induced chronic colitis mouse model. Our data show that chronic colitis is accompanied by the decrease of the serum tryptophan level and the depletion of the intestinal ...

Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß2 subunit of LFA1 family (ß2 integrins ...

consist of a backbone of (1→3)- and/or (1→4)-linked α-L-fucopyranose residues that may be substituted with sulfate (SO3-) on C-2, C-3, or C-4 and acetyl groups at C-4 on the main chain or may have short fucoside side chains that are usually linked from the O-4 of one or several of the fucopyranose backbone residues. FCSPs are known to exhibit crucial biological activities including anti-tumor activity. Although differently extracted, purified, fucose-rich, modified fucoidans have been reported to exert bioactive properties such as anti-coagulant and enhance immune response activity, few studies have investigated the bioactivity of unfractionated FCSPs, notably FCSPs extracted using milder and fewer processing steps. Crude fucoidan from Sargassum sp. and Fucus vesiculosus were examined for their bioactivity against lung and skin cancer cell lines in both in vitro and in vivo studies. This study showed that unfractionated FCSPs hinder the in vitro proliferation of Lewis lung carcinoma and ...

1 Cederberg BM, Gray GR. N-Acetyl-D-glucosamine binding lectins. A model system for the study of binding specificity. Anal Biochem. Oct 15, 1979; 99 (1): 221-30. DOI:10.1016/0003-2697(79)90067-8.. 2 Freed DLJ. Do dietary lectins cause disease? BMJ. Apr 17, 1999; 318 (7190): 1023-4. DOI: 10.1136/bmj.318.7190.1023.. 3 Houser J, Komarek J, Kostlanova N, et. al. A soluble fucose-specific lectin from Aspergillus fumigatus conidia-structure, specificity and possible role in fungal pathogenicity. PLoS One. Dec 10, 2013; 8 (12): e83077. DOI: 10.1371/journal.pone.0083077.. 4 Criado MT, Ferreiros CM. Selective interaction of a Fucus vesiculosus lectin-like mucopolysaccharide with several Candida species. Ann Microbiol (Paris). Mar-Apr 1983; 134A (2): 149-54. DOI: 10.1016/S0769-2609(83)80074-X.. 5 Hankins CN, Kindinger JI, Shannon LM. Legume Lectins: I. Immunological Cross-Reactions between the Enzymic Lectin from Mung Beans and other Well Characterized Legume Lectins. Plant Physiol. Jul 1979; 64 (1): ...

The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.

During this years annual meeting, Seattle Genetics and Unum Therapeutics presented pre-clinical data evaluating combination treatment with Antibody-Coupled T cell Receptor (ACTR) engineered autologous T cells and an antibody targeting B-cell maturation antigen or BCMA.. The ACTR technology enables the programming of a patients immune system to attack tumor cells when co-administered with tumor-specific therapeutic antibodies. The pre-clinical data support clinical evaluation of a humanized non-fucosylated anti-BCMA antibody, known as SEA-BCMA, being developed using Seattle Genetics novel sugar-engineered antibody (SEA) technology, and ACTR T cell combination treatment in multiple myeloma patients.. Designed to engage the Fc domain of therapeutic antibodies, the ACTR technology, is a universal, engineered T cell therapy consisting of the extracellular domain of human CD16 and the intracellular T cell co-stimulatory and signal domain.. One presentation highlighted data from pre-clincal studies ...

Researchers at A*STAR, collaborating with other Singapore institutions, have developed a technique that improves interactions between antibodies and natural killer (NK) cells to strengthen the bodys immune system in the fight against tumor cells.. The preliminary study findings, titled Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies. were published in the Biotechnology Journal.. Global cancer statistics indicate that cancer affected an estimated 12.7 million individuals as of 2008, without including non-melanoma skin cancers and other non-invasive cancers. The disease involves abnormal growth of cells to form tumors with the ability to invade other parts of the body in a process called metastasis.. From the therapeutic standpoint, several treatment options exist, including surgery, chemotherapy, and radiation therapy. Another type of therapy that continues to be an area of active research is ...

INTRODUCTION. In 1999 the Nobel Prize in Physiology or Medicine was awarded to Gunter Blobel for the discovery that proteins have intrinsic signals that govern their transport and localisation in the cell. It was based on this research into how cells use these signals that the functions of glycoproteins were discovered.. Glycoproteins are molecules that have a sugars and protein bonded together. The sugar chains of glycoproteins play a role in determining their destination in the cell or body. The bonding of sugars to proteins to form glycoproteins significantly alters the properties of these proteins.. There are eight sugars that predominantly bond with proteins to form glycoproteins, these are: xylose, galactose, glucose, mannose, fucose, N-acetylgalactosamine, N-acetylglucosamine and N-acetylneuraminic acid.. WHAT ARE THE FUNCTIONS OF GLYCOPROTEINS?. Glycans contain biologic information and are the specific sequence of sugars in a glycoprotein that determine its recognition and interaction ...

BioAssay record AID 36841 submitted by ChEMBL: Inhibition of the enzyme alpha-L-fucosidase from bovine epididymis was determined at 1 mM concentration of the compound; No inhibition.

SWISS-MODEL Template Library (SMTL) entry for 1lgb. INTERACTION OF A LEGUME LECTIN WITH THE N2 FRAGMENT OF HUMAN LACTOTRANSFERRIN OR WITH THE ISOLATED BIANTENNARY GLYCOPEPTIDE: ROLE OF THE FUCOSE MOIETY

Accepted name: D-arabinose 1-dehydrogenase [NAD(P)+]. Reaction: D-arabinose + NAD(P)+ = D-arabinono-1,4-lactone + NAD(P)H + H+. For diagram of reaction click here.. Other name(s): D-arabinose 1-dehydrogenase [NAD(P)]. Systematic name: D-arabinose:NAD(P)+ 1-oxidoreductase. Comments: Also acts on L-galactose, 6-deoxy- and 3,6-dideoxy-L-galactose.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 37250-48-9. References:. 1. Cline, A.L. and Hu, A.S.L. The isolation of three sugar dehydrogenases from a psuedomonad. J. Biol. Chem. 240 (1965) 4488-4492. [PMID: 5845847]. 2. Cline, A.L. and Hu, A.S.L. Enzymatic characterization and comparison of three sugar dehydrogenases from a pseudomonad. J. Biol. Chem. 240 (1965) 4493-4497. [PMID: 5845848]. 3. Cline, A.L. and Hu, A.S.L. Some physical properties of three sugar dehydrogenases from a pseudomonad. J. Biol. Chem. 240 (1965) 4498-4502. [PMID: 5845849]. ...

Adriaens, P. (2011). Sterna aurantia Gray, 1831. Accessed through: World Register of Marine Species at http://www.marinespecies.org/aphia.php?p=taxdetails&id=343926 on 2017-12- ...

GLYcoDiag provide reagents dedicated for glycosciences research: Natural and recombinant lectins. LEctPROFILE products, lectin gels, neoglycoproteins

GLYcoDiag provide reagents dedicated for glycosciences research: Natural and recombinant lectins. LEctPROFILE products, lectin gels, neoglycoproteins