Francisella philomiragia ATCC ® 25017D-5™ Designation: Genomic DNA from Francisella philomiragia strain O#319-036 TypeStrain=False Application:

Prior to this study, relatively few strains of Francisella had been genome-sequenced. Previously published Francisella genome sequences were largely restricted to the zoonotic agent F. tularensis. Only limited data were available for other members of the Francisella genus, including F. philomiragia, an opportunistic pathogen of humans, F. noatunensis, a serious pathogen of farmed fish, and other less well described endosymbiotic species. We determined the phylogenetic relationships of all known Francisella species, including some for which the phylogenetic positions were previously uncertain. The genus Francisella could be divided into two main genetic clades: one included F. tularensis, F. novicida, F. hispaniensis and Wolbachia persica, and another included F. philomiragia and F. noatunensis. Some Francisella species were found to have significant recombination frequencies. However, the fish pathogen F. noatunensis subsp. noatunensis was an exception due to it exhibiting a highly clonal population

The 16S ribosomal DNAs (rDNAs) of two strains of Francisella tularensis and one strain of Francisella philomiragia were sequenced. On the basis of phylogenetic analysis data, the genus Francisella was placed in the γ subclass of the Proteobacteria. The most closely related organism was the intracellular bacterium Wolbachia persica. The sequenced 16S rDNA molecules of the Francisella species exhibited very high levels of similarity (98.5 to 99.9%). Two variable regions, comprising 390 to 450 nucleotides of the 16S rDNA molecules of 17 additional Francisella strains, including members of the species F. tularensis and F. philomiragia, were also sequenced. At most, six nucleotide differences were observed among the sequences of the F. tularensis strains. The sequence of Francisella novicida was virtually identical to the sequences of the F. tularensis strains, thereby supporting the hypothesis that these organisms are members of the same species. On the basis of the observed differences, primer pairs were

ABSTRACT: Specific identification and differentiation of the 2 subspecies of the fish pathogen Francisella noatunensis, namely, F. noatunensis subsp. noatunensis and F. noatunensis subsp. orientalis, remains a major diagnostic challenge. Following whole-genome sequencing and analysis of representatives of all major subclades of the genus Francisella, specific genomic regions were identified for each of the subspecies of this fish pathogen. Two specific real-time quantitative PCR assays, directed at hypothetical genes within these regions were developed. Specificity was confirmed by lack of signal and cross-reactivity with the closest relative, F. philomiragia, and other common bacterial fish pathogens. Both assays, used either as monoplex or multiplex, have a limit of detection of 10 genome equivalents. The quantitative sensitivity of the assays was not affected by the presence of kidney tissues or DNA from Atlantic cod Gadus morhua or tilapia Oreochromis sp. ...

Sulyok Kinga M., Kreizinger Zsuzsa, Bekő Katinka, Forró Barbara, Marton Szilvia, Bányai Krisztián, Catania Salvatore, Ellis Christine, Bradbury Janet, Olaogun Olusola M., Kovács Áron B., Cserép Tibor, Gyuranecz Miklós: Development of Molecular Methods for the Rapid Differentiation of Mycoplasma gallisepticum Vaccine Strains from Field Isolates, JOURNAL OF CLINICAL MICROBIOLOGY 57: (6) e01084- ...

Ciliates of the genus Euplotes are commonly found in polar environments, and different species isolated from Arctic and Antarctic coastal seawaters are currently studied for their genome evolution and adaptation. In analyzing whole genome sequences of a wild-type E. petzi strain collected from Terra Nova Bay (Antarctica), it appeared that more than 3% of the assembled contigs had a bacterial origin and overlapped (one contig containing rDNA operon included) with DNA sequences of the gammaproteobacterium Francisella (represented by extremely infectious species to a wide array of different organisms man included). Given that an Euplotes species of temperate seawaters, E. raikovi, has already been found to host a Francisella species (namely F. endociliophora), we searched for and succeeded in isolating Francisella-like endosymbionts from E. petzi cells. Colonies of these endosymbionts (grown optimally at a temperature range from 4 to maximum 30 °C) have been analyzed for their genome and found to ...

Aims: This study was conducted to clarify the taxonomic status of Francisella sp. strain Ehime‐1, a fish pathogen, in relation… Expand ...

A screen of 20 compounds identified small molecule adjuvants capable of potentiating antibiotic activity against Francisella philomiragia. Analogue synthesis of an initial hit compound led to the discovery of a potentially new class of small molecule adjuvants containing an indole core. The lead compound was 2016 MedChemComm Hot Articles Phenotypic Drug Discovery Antibiotic resistance

Definition : Molecular assay reagents intended to identify species of Francisella, a genus of gram-negative coccoid or rodlike bacteria, by detecting specific information of the nucleic acid of the target bacteria. Some species of these bacteria are the etiologic agents of tularemia, a disease characterized by malaise, fever, chills, and weakness; these bacteria are typically transmitted by wild animals.. Entry Terms : Francisella Species Detection/Identification Reagents , Tularemia Antibody Detection/Identification Reagents , Francisella tularensis Reagents, Identification , Reagents, Molecular Assay, Infection, Bacteria, Francisella Species. UMDC code : 21555 ...

CRISPR/Cas9 systems are nowadays applied extensively to effect genome editing in various organisms including plants. CRISPR from Prevotella and Francisella 1 (Cpf1) is a newly characterized RNA-guided endonuclease that has two distinct features as compared to Cas9. First, Cpf1 utilizes a thymidine-rich protospacer adjacent motif (PAM) while Cas9 prefers a guanidine-rich PAM. Cpf1 could be used as a sequence-specific nuclease to target AT-rich regions of a genome that Cas9 had difficulty accessing. Second, Cpf1 generates DNA ends with a 5′ overhang, whereas Cas9 creates blunt DNA ends after cleavage.

Peptidoglycan is a macromolecule made of long aminosugar strands cross-linked by short peptides. It forms the cell wall in bacteria surrounding the cytoplasmic membrane. The glycan strands are typically comprised of repeating N-acetylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc) disaccharides. Each MurNAc is linked to a peptide of three to five amino acid residues. Disaccharide subunits are first assembled on the cytoplasmic side of the bacterial membrane on a polyisoprenoid anchor (lipid I and II). Polymerization of disaccharide subunits by transglycosylases and cross-linking of glycan strands by transpeptidases occur on the other side of the membrane. Bacterial cell wall biosynthesis inhibitors form a major class of antibiotics ...

Objective: The purpose of this experiment was to compare the Sorin KIDS D131 and the Terumo Capiox AF02 pediatric arterial… Expand ...

Francisella tularensis is the etiologic agent of tularemia, a zoonotic disease maintained in the environment by rodents, lagamorphs and their ectoparasites. The...

Antibiotic resistance in Francisella novicida, a close relative of a bacterium that causes tularemia, is promoted by a system of genes that bacteria use to fend off viruses

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Created attachment 341652 [details] SPEC file I want package val(a)ide but before need apply a patch to vala lib such as (shell script) : ________________________________________________________________________ vala_dir=$(dirname $(which valac))/../share/vala patch -p0 -d $vala_dir , $(cd `dirname $0` && pwd)/gtk+-2.0.vapi.diff ________________________________________________________________________ The .spec add an ...

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Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Francisellaceae; Francisella; Francisella ...

Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Francisellaceae; Francisella; Francisella ...

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Havent checked yet but wouldnt be surprised if all of the FTN related applications in Debian that have network capability, like binkd or ifcico (part of ifmail) for instance, do not have IPv6 capability as yet ...

F. tularensis, a small, nonmotile, nonsporulating, gram-negative, coccobacillus is currently one of the most infectious bacteria known and is the causative agent of the systemic disease tularemia, a potentially severe and fatal disease in humans. F. tularensis is also characterized by its resilience to environmental conditions and its ease of dissemination. For these reasons, F. tularensis poses a serious threat to laboratory workers who handle the organism and is a bioterrorism concern.. The gamma-protobacterial family Francisellaceae consists of two genera: Francisella tularensis and Francisella philomiragia. F. tularensis has four subspecies: tularensis (Type A), holarctica (Type B), mediaasiatica (geographically restricted to the central Asia) and novicida (a rare isolate initially isolated from a water source in Utah in 1951). F. tularensis subsp. tularensis, is the most virulent subspecies and is geographically restricted to the North American continent, with only one report of isolation ...

Francisella tularensis, the causative agent of tularemia, is a potent human and animal pathogen. Its principal survival mechanism is rapid intracellular multiplication. The mechanisms that enables it to multiply intracellularly have been ill-defined and the thesis focused on characterizing the outcome of the macrophage-Francisella interaction and also if the interactions differ between the various subspecies of F tularensis.. The nature of host cell death was examined and the correlation of macrophage killing with intramacrophage Francisella growth was investigated by in vitro infection of J774A.1 macrophages with either the live vaccine (LVS) strain of F. tularensis, belonging to subspecies holarctica, or the subspecies novicida strain U112 Macrophage entry was in both cases cytochalasin D-sensitive but the intramacrophage growth of the two Francisella strains led to distinct types of host cell death, i.e., apoptosis vs. necrosis.. The macrophage apoptosis induced by infection with the LVS ...

One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.

Looking for online definition of Francisella in the Medical Dictionary? Francisella explanation free. What is Francisella? Meaning of Francisella medical term. What does Francisella mean?

Francisella tularensis is one of the most virulent bacteria known and a Centers for Disease Control and Prevention Category A select agent. It is able to infect a variety of animals and insects and can persist in the environment, thus Francisella spp. must be able to survive in diverse environmental niches. However, F. tularensis has a surprising dearth of sensory and regulatory factors. Recent advancements in the field have identified new functions of encoded transcription factors and greatly expanded our understanding of virulence gene regulation. Here we review the current knowledge of environmental adaptation by F. tularensis, its transcriptional regulators and their relationship to animal virulence.

Francisella tularensis PdpD protein: a pathogenicity island protein required for full virulence and associates with homologues of the type VI secretion system; RefSeq YP_898955.1

SWISS-MODEL Repository entry for A0Q5E0 (RSMA_FRATN), Ribosomal RNA small subunit methyltransferase A. Francisella tularensis subsp novicida (strain U112)

Современное состояние классификации некоторых патогенных представителей родов Bacillus, Brucella, Burkholderia, Francisella, Vibrio, Yersinia

Researchers determined which cytokines induced the two activated antimicrobial macrophage effector reactions-resistance to infection and intracellular killing-and which cytokines shut down the killing. The author made inroads in the understanding of Francisella infections, devised a polymerase chain reaction-based detection system and an attenuated and subunit vaccine, and explored the use of immunomodulation with bacille-Calmette-Guerin (BCG) with great success. In this chapter the author seeks additional philanthropic funds to assist companies and academic investigators in the clinical development of new anti-TB drugs. The path is set for the foundation, and there is great excitement both within the foundation and the research community. Sequella, Inc., is working with investigators in universities around the world and has licensed some innovative, useful products for diagnosis and treatment of tuberculosis (TB). It is currently raising money to fund the development of its products and hopes to have

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Complete information for IGLC2 gene (Protein Coding), Immunoglobulin Lambda Constant 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

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Poté, co v roce 1999 získala titul Ph.D, byla jmenována odborným asistentem na MIT. Ve 29 letech byla nejmladší členkou fakulty, která zastávala funkci na této univerzitě. Duflo a Banerjee se o Indii zajímají od roku 1997. V roce 2003 zde provedla zkušební experiment, o nepřítomnosti učitelů, ve 120 školách, které provozují neziskové organizace. Jejím hlavním zájmem byla častá absence vyučujících. Povzbuzením učitelů, aby se každý den fotografovali se svými studenty, dokázala snížit jejich absenci. V roce 2003 spoluzaložila společnost Poverty Action Lab na MIT, která od té doby provedla přes 200 empirických výzkumů. Laboratoř sídlí v Chennai v Indii a na Pařížské ekonomické škole. V roce 2006 spolu s několika kolegy provedla Duflo v Indii další experiment. Ukázalo se, že nahrané projevy žen byly ochotněji přijímány ve vesnicích, které zažily ženské vůdce. Duflo se stále více přesvědčovala, že komunity podporující ...

Poglavitna vloga mielinske ovojnice je povečanje hitrosti potovanja akcijskega potenciala vzdolž nevrita. Po nemieliziniranem vlaknu potuje dražljaj nepretrgoma v obliki vala, po mieliniziranem vlaknu pa skokovito - poskakuje iz enega Ranvierovega zažetka na drugega. Mielinska ovojnica namreč ni zvezna; v enakih razmikih se pojavljajo vrzeli, ki niso obdani z mielinsko ovojnico (t. i. Ranvierovi zažetki), kjer je nevrit gol. Mielin močno poveča električno upornost vzdolž celične membrane ter zmanjša kapacitivnost. S tem mielinska ovojnica pomaga preprečevati, da bi električni dražljaj zapustil nevrit. Če se periferno vlakno prekine, predstavlja preostala mielinska ovojnica sled, kjer naj bi vlakno ponovno zaraslo. Vendar pa mielinska ovojnica ne zagotavlja optimalne regeneracije živčnega vlakna; nekatera regenerirana vlakna ne najdejo ponovno poti do pravega mišičnega vlakna, nekateri poškodovani motorični nevroni obkrajnega živčevja pa ponovno sploh ne zarastejo. Če sta ...

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Building a high performing building requires project teams to integrate their knowledge, their organization and their information, leveraging metrics, models (energy, BIM, cost and schedule), co-location and collaboration, and production management. This model, the Simple Framework for Integrating Project Delivery was validated in action on the UCSF Mission Bay Hospitals project. The senior project leaders created an integrated community that employed Lean and Virtual Design and Construction methods to create solutions in the best interest of the project.. ...