VCF (voltage clamp fluorimetry) provides a powerful technique to observe real-time conformational changes that are associated with ion channel gating. The present review highlights the insights such experiments have provided in understanding Kv (voltage-gated potassium) channel gating, with particular emphasis on the study of mammalian Kv1 channels. Further applications of VCF that would contribute to our understanding of the modulation of Kv channels in health and disease are also discussed.. ...
Purified Universal Kinase Fluorometric Assay Kit from Creative Biomart. Universal Kinase Fluorometric Assay Kit can be used for research.
Purified LDH-Cytotoxicity Fluorometric Assay Kit from Creative Biomart. LDH-Cytotoxicity Fluorometric Assay Kit can be used for research.
By coupling the Fenton reaction with the glucose oxidase (GOD)-catalyzed reaction, we have developed a fluorometric method for the determination of glucose; the linear response range and the detection limit are 0.108-2.59 microg/ml and 0.0432 microg/ml, respectively. This approach is selective and has the advantages of using inexpensive reagents and avoiding interferences. Satisfactory results were obtained for the determination of glucose in human serum samples.
Ethanol Assay Kit: Colorimetric & Fluorometric Assay for Accurate Quantification of Ethanol in a variety of Biological Samples such as Serum, Plasma, other Body Fluids, Food, Beverages, Growth Media etc. Rapid, Simple & Sensitive. Detection Range: ~10-800
Fructose Colorimetric/Fluorometric Assay Kit; Compatible with high-throughput handling systems. Bulk and Prepack available at Sigmaaldrich.com.
Renin Activity Fluorometric Assay Kit: simple, rapid & convenient method to detect renin activity as low as 0.75 U/ml. 100 assays.
Described and claimed an interchangeable tip - open cell fluorometer comprising: a housing and a fluorometric probe tip can be replaced connected to the housing, said probe tip including a probe fluorescent probe housing, the probe needle probe housing and defining open cell containing probe optical arrangement, the probe optical arrangement including an excitation source and a fluorescence detector wherein the excitation source is aimed at the fluorescence detector according to that formula can be opened sample in the sample cell by fluorescence detection. Also it claimed using such interchangeable tip - sample method of a natural or industrial water system one or more fluorophores emit fluorescent signals open cell fluorometer to detect from. When combined with the controller, the fluorometer capable of monitoring and optionally controlling an industrial process or system.
Two years after the acquisition of PTI, HORIBA is proud to introduce the new PTI QuantaMaster™ 8000 series of fluorometers. The new PTI QuantaMaster 8000 series modular research fluorometers from HORIBA Scientific offer the worlds highest guaranteed sensitivity specification, plus many unique benefits. Only the HORIBA Fluorolog 3, also from HORIBA Scientific, matches the sensitivity of the new QuantaMaster 8000 series.
An automatic analyzing apparatus for effecting chemical analyses for various sample liquids such as blood, urine, and the like, comprising a sample delivery pump for metering a sample liquid into a reaction cuvette, a reagent delivery pump for delivering to the reaction cuvette a given amount of a given reagent selected from a plurality of reagents contained in a reagent cassette, to form a test liquid, a feed mechanism for successively supplying reaction cuvettes along a circular reaction line, a plurality of photometering sections arranged along the reaction line for effecting a plurality of photometric and/or nephelometric and/or fluorometric measurements for each test liquid at different time instances to produce a plurality of photometric results, and circuitry for receiving the photometric results and selecting therefrom given quantitative analytical data of a given test item.
An automatic analyzing apparatus for effecting chemical analyses for various sample liquids such as blood, urine, and the like, comprising a sample delivery pump for metering a sample liquid into a reaction cuvette, a reagent delivery pump for delivering to the reaction cuvette a given amount of a given reagent selected from a plurality of reagents contained in a reagent cassette, to form a test liquid, a feed mechanism for successively supplying reaction cuvettes along a circular reaction line, a plurality of photometering sections arranged along the reaction line for effecting a plurality of photometric and/or nephelometric and/or fluorometric measurements for each test liquid at different time instances to produce a plurality of photometric results, and circuitry for receiving the photometric results and selecting therefrom given quantitative analytical data of a given test item.
References. 1. Couser, W. G.; Remuzzi, G.; Mendis, S.; Tonelli, M. Kidney Int. 2011, 80, 1258-70. [ Links ] 2. Levey, A. S.; Atkins, R.; Coresh, J.; Cohen, E. P.; Collins, A. J.; Eckardt, K. U.; Nahas, M. E.; Jaber, B. L.; Jadoul, M.; Levin, A.; Powe, N. R.; Rossert, J.; Wheeler, D. C.; Lameire, N.; Eknoyan, G. Kidney Int . 2007, 72, 247-259. [ Links ] 3. John, R.; Webb, M.; Young, A.; Stevens, P. E. Am J Kidney Dis. 2004, 43, 825-835. [ Links ] 4. National Kidney, F. Am J Kidney Dis . 2002, 39, 1-266. [ Links ] 5. Fraser, S. D.; Roderick, P. J.; McIntyre, N. J.; Harris, S.; McIntyre, C.; Fluck, R.; Taal, M. W. PLoS One. 2014, 9, 98261. [ Links ] 6. Tangri, N.; Stevens, L. A.; Griffith, J.; Tighiouart, H.; Djurdjev, O.; Naimark, D.; Levin, A. ; Levey, A. S. JAMA. 2011, 305, 1553-1559, http://dx.doi.org/10.1001/jama.2011.451. [ Links ] 7. Johnson, D. W. Intern Med J. 2004, 34, 50-57. [ Links ] 8. Cravedi, P.; Ruggenenti, P.; Remuzzi, G. Nat Rev Nephrol. 2012, 8, 301-306. ...
View Notes - fulltext9 from SPECTOGRAP 545 at AIB College of Business. Frequency Domain Fluorometry with Pulsed Light-Emitting Diodes P ETR H ERMAN AND J AROSLAV V ECER Faculty of Mathematics and
And metastasis by fluorometry and for skeletal abnormalities by X-ray. High-magnification imaging of the GFP-expressing tumors was carried out with a Leica
A method is provided to fluorometrically determine a ligand in an assay solution containing the ligand, reagent system and a fluorescer wherein the intensity of the fluorescer emitted by the assay sol
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
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The LI-600 Porometer/Fluorometer takes quick, precise measurements of stomatal conductance and chlorophyll a fluorescence over the same leaf area.
Discussion The Chelsea Minitracka fluorometer supplied with your Sea-Bird CTD measures Chlorophyll-a concentrations in the range of 0.03 to 100 μg/l with a linear output of 0 to +4 volts. In our Seasoft V2 suite of programs, edit the CTD configuration
For over 40 years Turner Designs has specialized in providing sensitive, reliable and easy-to-use fluorometers to measure the properties of water for environmental and industrial applications.
Fluorescent Dyes , Enzyme Detection Reagents , Caspase 3 (Apopain) Substrate 1r-z, fluorogenic; Rh110 (rhodamine 110)-derived caspase substrates are probably the most sensitive indicators widely used for the fluorimetric detection of various caspase activities. Cleavage of Rh110 peptides by caspases generates strongly fluorescent Rh110 that is monitored fluorimetrically at 510-530 nm with excitation of 488 nm, the most common excitation light source used in fluorescence instruments.; Caspase-3 substrate and caspase-7 substrate; (Z-DEVD)2-Rh110; z-(Asp-Glu-Val-Asp)2-Rh110
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Plate readers are used in a laboratory to detect physical, chemical or biological changes within a sample. This is usually measured on multi-well plate, typically a 96 well plate and it does so via measurement of light. When buying a used plate reader you do want to consider a few things, but the biggest factor you want to consider is what type of detection mode it uses. A lot of the plate readers we have in stock can actually handle a variety of detection modes, absorbance, fluorescence, luminescence and you want to make sure the one it has works for the applications you want to use it for. The more popular plate readers that BioSurplus carries, we have BMG Lab Tech, Perkin Elmer, Molecular Devices, as well as Thermo Scientific. When purchasing a used plate reader from BioSurplus you can rest assured that we made sure the instrument was working when we acquired it, that we tested on the way in and were testing it before it gets out to our customers, so it has the BioSurplus stamp on it." ...
A fluorometer for sensing the fluorescence of a sample utilizes an optical energy source for exciting a sample to be tested and an optical energy detector for detecting the emitted energy from the excited sample. Drive electronics are used for positioning the sample with respect to the optical components allowing a plurality of sample regions to be tested. A processor is utilized to control the operation of the test in accordance with test instructions and for processing the emitted energy detected from the sample to determine test results. A ROM chip socket accepts a plurality of ROM chips, wherein each ROM chip stores test data sets for one or more test types to be performed. ROM chips can be swapped to allow the fluorometer to be configured and reconfigured to perform a plurality of different tests. A communications interface facilitates the sharing of test information between the fluorometer and external entities.
* found in: BODChek™ Portable Fluorometer Kit, AlgaeChek™ Ultra Portable Fluorometer Kit, AlgaeChek™ Portable Fluorometer Kit, Portable Detection to..
L-Alanine Assay Kit (ab83394). Sensitivity: 24 μg/mL. Colorimetric or fluorometric detection of alanine in a variety of biological samples in 40 min.
A. Identify compound like morphine, quinine, riboflavin which produced fluroscence.. B. Flourescent indicator. C. Identify drug because specific drug have specific wave length radiation is emitted.. ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
BioAssay record AID 1095409 submitted by ChEMBL: Inhibition of Homo sapiens (human) NEU1 expressed in HEK293 cells using 4MU-NeuAc as substrate at 1 mM after 10 to 30 min by fluorometric analysis.
BioAssay record AID 364025 submitted by ChEMBL: Induction of apoptosis in mouse TLT cells assessed as effect on caspase 3 activity at 100 ug/ml after 24 hrs by fluorometric assay in presence of 2 mM vitamin C.
3ZLY: The Use of Virtual Screening and Differential Scanning Fluorimetry for the Rapid Identification of Fragments Active Against Mek1.
[127 Pages Report] Check for Discount on Global Plate Reader Market Professional Survey Report 2017 report by QYResearch Group. ...
We offer a range of options for adding a microscope to any of our steady state fluorometers. We can supply a complete system including a microscope,...
Amanda had it freeze because the file was "too big" 12/2013. It was 1.3 MB, and froze upon saving. She gave it 5 min to save, but the program said "not responding" in the force quit box. The file produced after force quitting was broken: the data was gone when she tried to open it again. Amanda has done bigger files before, so this was a little surprising. Customer support said that it can crash if you have files that are too big. Break your runs down into different files if you are worried. Amanda also suggests giving it as long as possible; maybe it would have come back to life ...
β-Secretase (BACE) is a membrane-bound aspartyl protease that cleaves the amyloid precursor protein and is consequently an excellent target for anti-amyloid therapy in the treatment of Alzheimers disease. Finding inhibitors of β-secretase is one of the major goals of Alzheimers disease drug development. PromoKines β-Secretase Fluorometric Assay Kit provides a convenient, non-radioactive system for detecting β-Secretase activity in biological samples. The kit provides active β-Secretase as positive control, β-Secretase inhibitor as negative control, optimized peptide substrate (conjugated to two reporter molecules), and buffers for convenient measurement of β-Secretase activity in mammalian samples. β-Secretase inhibitors are also available separately. ...
Creative Proteomics offer Aldehyde Dehydrogenase Activity Fluorometric Assay Kit. We are specialized in manufacturing Assay Kits.
In a message dated 96-04-08 19:24:31 EDT, rroxby at maine.maine.edu (Robert Roxby) writes: ,Hi, , Im looking for a general purpose fluorometer. I want to be able to ,vary excitation and emission wavelengths, whether by filters or ,monochrometers. I dont want an instrument that is specialized for ,measuring DNA concentrations. I thought Hoefer made one, but Im having ,trouble finding them. Any suggestions? Thanks. , , Turner Deisngs has a filter fluorometer with a quick change filter holder that holds four sets of fitlers. You can have additional ones if you want. Light source is easily changed if you need it. Abotu $5000. Check out molecular probes info ont their SYBR dyes and other applications. Hoefer is now part of Pharmacia, and do have a great $2500 Hoechst dye fluorometer. Turner Designs can be contacted at: mfeld at ix.netcom.com (Pam Mayerfeld) Walt Schick ...
Microfluorometric analysis of C1- permeability and its relation to oscillatory Ca2+ signalling in glucose-stimulated pancreatic beta cells ...
Fig. 2 B), consistent with negative cooperativity between sites in the same subunit (Sweet and Cox, 2008). In contrast, another group performing similar experiments at +50 mV concluded that positive cooperativity exists between the two binding sites (Qian et al., 2006). In the latter case, positive cooperativity could potentially be accounted for if both Ca2+ sites were coupled to the voltage sensor because the voltage sensor is not held in a resting state at +50 mV. However Sweet and Cox (2008) concluded that the RCK1 site is solely responsible for Ca2+-sensor/voltage-sensor coupling. A recent study combining Ca2+ site mutations and voltage clamp fluorometry to monitor the effects of Ca2+ on steady-state voltage-sensor activation and Po reached conclusions similar to that of Sweet and Cox (2008) regarding intrasubunit cooperativity and Ca2+-sensor/voltage-sensor coupling (Savalli et al., 2012). However, this study also suggested that Ca2+ site mutations may have effects other than elimination ...
Methodology provided: Techniques required for the quantitative use of fluorescent protein sensors in mitochondria in vitro, in organello and in vivo (i.e. in planta) will be contributed. This includes the characterisation and usage of redox sensors for glutathione, hydrogen peroxide and NADH/NAD, complemented by probes for pH, calcium and ATP/ADP. Confocal microscopy and FLIM is used to study functional and structural dynamics of individual mitochondria. Highly intact, functional mitochondria can be prepared from various plant tissues as an in organello system to dissect mitochondrial redox metabolism and regulation by combining classical bioenergetic assays with fluorometric measurements of the sensors. The majority of methods are directly applicable beyond plants to a wide range of ...
An integrated microfluidic system that coupled lysis of two cell lines: L929 fibroblasts and A549 epithelial cells, with fluorescence-based enzyme assay was developed to determine β-glucocerebrosidase
Gas-phase H2O2, organic peroxides and carbonyl compounds were determined at various sites from Mid-July to early August 1998 during the BERLIOZ campaign in Germany. The sites were located northwest of Berlin and were chosen to determine pollutants downwind of the city emissions during a summer smog episode. Hydrogen peroxide (H2O2), methyl hydroperoxide (MHP, CH3OOH) and occasionally hydroxymethyl hydroperoxide (HMHP, HOCH2OOH) were quantified in air samples by commercial fluorimetric methods and classical HPLC with post-column derivatisation by horseradish peroxidase/p-hydroxyphenyl acetic acid and fluorimetric detection. Carbonyl compounds were determined in ambient air by a novel method based on O-pentafluorobenzyl hydroxylamine as derivatisation agent. Mixing ratio profiles of the hydroperoxides and the carbonyl compounds are reported for the intensive phase of the campaign, 20-21 July, 1998. Peroxides showed pronounced diurnal variations with peak mixing ratios in the early afternoon. At ...
A method for measuring total antioxidant activity of antioxidants utilizes BODIPY dyes which fluoresce in the visible region of the electromagnetic spectrum as a fluorescent reporter.
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
The 10AU Field Fluorometer is a rugged, field-portable instrument that can be set up for continuous-flow monitoring or discrete sample analyses.
Kurland, J I.; Traganos, F; Darzynkiewicz, Z; and Moore, M A., "Macrophage-mediated cytostasis of neoplastic hemopoietic cells. Cytofluorometric analysis of the reversible cell cycle block." (1978). Subject Strain Bibliography 1978. 1662 ...
The AccuClear® and AccuBlue® DNA Quantitation kits are designed for use with fluorescence 96-well plate readers. AccuClear®, AccuBlue® NextGen and AccuBlue® High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). AccuBlue® Broad Range requires an instrument equipped to read blue fluorescence emission (Ex/Em 350/460 nm).. These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor™-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kit, which is designed for use on that instrument.. The AccuGreen™ High Sensitivity DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA High Sensitivity assay, and is a direct replacement for the ...
Enolase Activity Assay Kit is a quantitative colormetric/fluorometric determination of Enolase Activity. (KA3839) - Products - Abnova
A fluorometric method for the estimation of the vitamin B1 content in food and biological materials is described. Acid hydrolysis is followed by enzymatic treatment to liberate the vitamin B1 of the sample. Interfering substances in the extract are removed to a great extent by chromatography on Amberlite CG 50, a weakly acidic cation exchanger. After oxidation and extraction into an organic medium, the fluorescence of thiochrome is measured. The procedure is simple, reproducible and not susceptible to interference. The recoveries are good.