Currently there are no approved biomarkers for the pre-symptomatic diagnosis of Alzheimers disease (AD). Cathepsin-D (Cat-D) is a lysosomal protease that is present at elevated levels in amyloid plaques and neurons in patients with AD and is also elevated in some cancers. We have developed a magnetic resonance imaging (MRI)/fluorescent contrast agent to detect Cat-D enzymatic activity. The purpose of this study was to investigate the cellular and tissue uptake of this MRI/fluorescent contrast agent. The agent consists of an MRI probe [DOTA-caged metal ion (Gd3+ or Tm3+)] and a fluorescent probe coupled to a cell-penetrating-peptide sequence by a Cat-D recognition site. The relaxivity of Gd3+-DOTA-CAT(cleaved) was measured in 10% heat-treated bovine serum albumin (BSA) phantoms to assess contrast efficacy at magnetic fields ranging from 0.24 mT to 9.4 T. In vitro, Tm3+-DOTA-CAT was added to neuronal SN56 cells over-expressing Cat-D and live-cell confocal microscropy was performed at 30 min. ...

TY - JOUR. T1 - Myosin V walks hand-over-hand. T2 - Single fluorophore imaging with 1.5-nm localization. AU - Yildiz, Ahmet. AU - Forkey, Joseph N.. AU - McKinney, Sean A.. AU - Ha, Taekjip. AU - Goldman, Yale E.. AU - Selvin, Paul R.. PY - 2003/6/27. Y1 - 2003/6/27. N2 - Myosin V is a dimeric molecular motor that moves processively on actin, with the center of mass moving ±37 nanometers for each adenosine triphosphate hydrolyzed. We have labeled myosin V with a single fluorophore at different positions in the light-chain domain and measured the step size with a standard deviation of ,1.5 nanometers, with 0.5-second temporal resolution, and observation times of minutes. The step size alternates between 37 + 2x nm and 37 - 2x, where x is the distance along the direction of motion between the dye and the midpoint between the two heads. These results strongly support a hand-over-hand model of motility, not an inchworm model.. AB - Myosin V is a dimeric molecular motor that moves processively on ...

Fluorescent probes are useful tools to investigate specific subcellular components in cells, tissues and organisms. A classic application of fluorescent ligands of a specific GPCR (G-protein coupled receptors) is the investigation of the receptor location, and, if their binding is reversible, it could provide pharmacological information such as affinity and proximity between interacting molecules. In cultured cell systems, methods based on fluorescence have permitted to observe the receptor cycling and the formation of oligomeric receptor complexes. In high throughput screening, fluorescent ligands represents a safer, more powerful and more versatile alternative to radioligands. [1] The introduction of the bulky fluorophore into a small molecule ligand could lead to deep modifications not only from a physicochemical point of view but also in its pharmacological properties. [2] In order to allow a correct interaction of the pharmacophore with its receptor, it is usually separated from the ...

Single molecule fluorescence measurements are used to probe the effects of GM1 in DPPC monolayers. Langmuir-Blodgett films of GM1 and DPPC were doped with ~10−8 mol% of the fluorescent lipid probe, BODIPY-PC, and transferred onto glass substrates at 23 mN/m. As shown previously, the individual orientation of each BODIPY-PC probe in the membrane can be measured using defocused polarized total internal reflection fluorescence microscopy, revealing changes in film properties at the molecular level. Here, BODIPY-PC tilt angle histograms are used to characterize the effects of GM1 in DPPC films from 0.05 mol% to 100 mol% GM1. At high GM1 levels (,5 mol% GM1), trends in the single molecule measurements agree with previous bulk measurements showing the turnover from condensing to expanding influence of GM1 at ~20 mol%, thus validating the single molecule approach. At biologically relevant, low concentrations of GM1 (,5 mol% GM1), where bulk fluorescence measurements are less informative, the single ...

TMA-DPH (1-(4-trimethylammonium)-6-phenyl-1,3,5-hexatriene), a hydrophobic fluorescent membrane probe, interacts with living cells by instantaneous incorporation into the plasma membrane, where it becomes fluorescent. It then follows the intracellular constitutive membrane traffic and acts as a bulk membrane marker of the endocytic pathway (Illinger, D., P. Poindron, P. Fonteneau, M. Modolell, and J. G. Kuhry. 1990. Biochim. Biophys. Acta. 1030:73-81; Illinger, D., P. Poindron, and J. G. Kuhry. 1991. Biol. Cell. 73:131-138). As such, TMA-DPH displays particular properties mainly due to partition between membranes and aqueous media. From these properties, original arguments can be inferred in favor of the maturation model for the endocytic pathway, against that of pre-existing compartments, in L929 cultured mouse fibroblasts. (a) TMA-DPH labeling is seen to progress from the cell periphery to perinuclear regions during endocytosis without any noticeable loss in fluorescence intensity; with a ...

购买CGP-54626A Fluorescent ligand (Red), a fluorescent GABAB antagonist。使用Abcam高品质的CGP-54626A Fluorescent ligand (Red)帮助您更快取得科研成果。

Spectral properties of carbocyanine dye 3-methyl-2-[3-methyl-2-(3-methyl-2,3-dihydro-1,3-benzothiazole-2-iliden)-1- butenyl]-1,3-benzothiazole-3-il iodide (Cyan betaiPr) in water solution, as well as in the presence of different types of double stranded DNA have been studied. While in water solution of free dye Cyan betaiPr stays mainly in monomeric form, in the presence of DNA the dye molecules form J-aggregates. The molecular structure of these J-aggregates causes the Davydov splitting of their absorption band, corresponding to the first electronic transition. A study of site-specificity showed that in the presence of poly (dA/dT) the majority of Cyan betaiPr molecules form J-aggregates, while in the presence of poly (dGC/dGC) dye molecules stay mainly in monomeric form and in presence of chicken erythrocytes DNA both J-aggregate and monomeric forms of dye are present. We suppose that Cyan betaiPr molecules aggregate in DNA groove, which serves as a template for J-aggregate forming. An ...

Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.

Calcium ions play an important role in cell function, acting as effectors and/or signaling molecules for a variety of cellular biochemical and physiological processes. The development of a variety of Ca2+-sensitive fluorescent indicators and advancements in imaging technology have enhanced the ability to follow both global and localized changes in intracellular Ca2+ at ever improving temporal and spatial resolution. Ratiometric Ca2+ indicators like fura-2 permit the measurement of intracellular Ca2+ concentration (Grynkiewicz et al., 1985). But, they tend to have a limited dynamic range and a significant fluorescence background, which reduce their sensitivity to small local increases in Ca2+. On the other hand, certain nonratiometric Ca2+ indicators, such as fluo-3, although not able to give a dynamic direct read-out of the Ca2+ concentration, have very low fluorescence when not bound to Ca2+ and have a significant increase in fluorescence intensity upon binding Ca2+ (e.g., ∼200 times for ...

N-Alkylation of a novel pyridine sensor results in pyridinium salts whose conformations are stabilised by pyridinium cation-π interactions resulting in a fluorescent response that can be used to sense the presence of alkylating agents in solution at low concentration.. ...

Fluorescence Dye 620-M with Streptavidin conjugate. All the Fluorescent Dye M series products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. Besides, in comparison with phycobiliprotein tandems, Fluorescent Dye M series possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications. (U0293) - Products - Abnova

FDSS is a kinetic plate reader in Fluorescence and Luminescence, and suitable for reading the kinetics of the living cells which those response, calcium flux, mobilization for example, is very fast and need to monitor its kinetic property. GPCR is one of the most highly used application field for FDSS kinetic reader monitoring the calcium transient of the living cells. From the basic fluorescence dye such like Fluo-4, Fura-2 and to more various type of fluorescence dye such like FRET, and Flash Luminescence (Aequorine etc.,) reading technology can be used in our Kinetic reading platform.. FDSS7000EX is a lamp&filter base High-end system, capable of 1536 cell based assay with multiple washing ability and 2 dispenser head setup for the sticky compounds. It has more flexibility in having multiple of reading settings. Ratiometric Fura-2, Single wavelength Fluo-3 type Calcium flux, mobilization reading are possible. Some dye which requires FRET can also be done by using the motorized emission filter ...

Fam Labeled Fluorogenic Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

We have examined the migration of murine macrophages from the vascular compartment to normal and inflammatory tissues by the adoptive transfer of resident peritoneal macrophages (RPM phi) fluorescently labeled with the hydrophobic dye 1,1-dioctadecyl 3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI). After initial labeling of the plasma membrane of RPM phi, the dye accumulated stably in intracellular vesicles of low density (rho = 1.042-1.045 kg/l) and cells remained viable in culture for 4 weeks. Like the normal monocyte, DiI-RPM phi, but not exudate-derived or fixed cells, migrated to peritoneal exudates, following i.v. adoptive transfer, by a mechanism inhibitable by an antibody to the type 3 complement receptor. In the absence of an inflammatory stimulus there was no migration to the peritoneal cavity, and DiI-RPM phi accumulated within 4 h in the red pulp and marginal zone of the spleen. By day 6 these cells still formed a tight ring of fluorescence in the marginal zone alone, ...

노인성 치매에서 가장 많은 비율을 차지하는 알츠하이머병 (Alzheimers disease) 환자의 뇌에는 이 질병의 핵심 표지자로 알려져 있는 아밀로이드 단백질 (amyloid β protein)이 쌓여있으며, 이 단백질이 질병의 발달과 연관성에 대한 많은 연구가 있다. 아밀로이드 단백질 연구에 있어서 조직염색 (tissue staining)을 통한 공초점 (confocal microscope) 그리고 이광자 현미경 (two-photon microscope) 촬영이 큰 역할을 하고 있으며, 이에 아밀로이드 단백질 특이한 형광염료 (fluorescence dye)의 중요성이 부각되었다. 본 연구에서는 기존의 아밀로이드 단백질 형광염료와는 다른 새로운 형광염료를 개발하였다. 형광염료내의 전자기부자 (donor)와 수락자 (acceptor)사이의 종류 그리고 두 분자 사이의 거리를 조절함으로써 조직염색 시 더 적은 자가형광을 내며, 더 특이적으로 아밀로이드 ...

Emitted Light Filtering - We find that many researchers have optimized imaging/filtering conditions for EtBr, which is a red dye. Please pay close attention to the filters; though EtBr filters will work if there are no other options, they may absorb a lot of emitted shorter wavelength light and therefore lower the observed sensitivity. A GFP/Sybr Green filter is preferred for Midori Green dyes.. Excitation Light Source - Though transilluminators that emit shorter wavelengths (254 nm) are very good for EtBr, they are not good for Midori Green dyes. We recommend UV tables with emissions at 306 nm or higher-the longer the better! For optimal results, use blue LED and blue/green LED light sources for Midori Green Advance and Midori Green Direct. Midori Green Xtra is not compatible with UV; it should be used with blue LED or blue/green LED illumination only.. Natural Light Exposure - Midori Green is sensitive to photo-bleaching effects from long exposure to direct (or even indirect) sunlight. We ...

I used single molecule fluorescence and fluorescence lifetime measurements of fluorescent dyes conjugated to DNA to better understand the photophysical and photochemical interactions between organic dye molecules and DNA. This process is important, because fluorescent dyes are frequently used as labels for DNA, but interactions with specific DNA bases can significantly affect the fluorescent properties of the dyes, leading from chromatic shifts all the way to fluorescence quenching. Thus, it is very important to obtain a full understanding of the photophysics of these interactions if cDNA or siRNAs are used as molecular probes. I studied DNA hairpins that were labeled with different numbers of red-emitting Atto655 dyes. I investigated three different samples: a DNA hairpin labeled with three dyes, one with two dyes, and one with only one dye attached to specific bases and well-known spatial separations. Sample preparation consisted of denaturing and annealing the short synthesized single strands ...

The use of labelling or staining agents has greatly assisted the study of complex biological interactions in the field of biology. In particular, fluorescent labelling of biomolecules has been demonstrated as an indispensable tool in many biological studies. Types of fluorescent labelling agents that are commonly used include conventional classes of organic fluorophores such as fluorescein and cyanine dyes, as well as newer types of inorganic nanoparticles such as QDs, and novel fluorescent latex/silica nanobeads. The newer classes of fluorescent labels are gaining increasing popularity in place of their predecessors due to their better optical properties such as possessing an enhanced photostability and a larger Stokes shift over conventional organic fluorophores, for example. This paper gives an overview of the recent advances on these luminescent nanomaterials with emphases on their optical characteristics that are crucial in fluorescence microscopy, both advantages and limitations in their ...

The RNAscope® Multiplex Fluorescent assays provide the same exceptional sensitivity as our singleplex assays, allowing single-molecule detection of up to four RNA targets simultaneously. The RNAscope® Multiplex Fluorescent assays are ideal for co-localization studies of any genes in nearly any tissue type using fluorescent labels. Advanced Cell Diagnostics offers two types of multiplex fluorescent assays. RNAscope® Fluorescent Assay, our first generation assay, is an all in one kit, ideal for fresh or fixed frozen tissues.

This application note describes the use of the SPECTRAmax GEMINI XS fluorescence microplate reader, which helps in studying the changes in levels of intracellular cAMP.

Recombinant Proteins , Streptavidin and Labeled Streptavidin , Streptavidin, HiLyte Fluor 555 conjugated; HiLyte Fluor555-streptavidin conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised streptavidin function. The spectrum of HiLyte Fluor555 is only slightly red-shifted compared to those of Cy3 dyes, resulting in an optimal match to filters designed for Cy3 dyes. The fluorescence of HiLyte Fluor555 can be observed at excitation/emission wavelength of 554 nm /570 nm. HiLyte Fluor555 is more photostable than Cy3 , providing researchers with additional time for capturing image. HiLyte Fluor555 - protein conjugates can sustain treatments during immunofluorescent staining, fluorescence in situ hybridization, flow cytometry and other biological applications without hydrolysis.

With respect to cellular analysis, the underlying principle of flow cytometry is that a cell suspension is focused into a single cell stream, which passes through a light source (typically a laser beam). The scattered and emitted fluorescent light (if the cells are fluorescently labeled) is subsequently measured using a range of detectors and these measurements are used to generate multi-parameter data sets that describe the physical characteristics of the cells and their fluorescent properties. The size and granularity of cells can be identified on the basis of their forward and side light scatter characteristics (FSc and SSc respectively). Their characteristics and/or expression of different proteins can be further defined by pre-staining with fluorescently-labeled antibodies or molecules that identify cellular components and / or integrity (viability) or, indeed, fluorescently-labeled proteins.. Flow cytometers can evaluate cells at an extremely rapid rate (up to several tens of thousands of ...

Fluorescence in situ hybridization (FISH) is a valuable cytogenetic technique for the detection and localization of specific DNA or RNA sequences on chromosomes. Typically, this technique can be used to define spatiotemporal expression patterns of target genes. Using fluorescent DNA probes with sequences complementary to the location of the chromosome of interest, gene expression patterns can be easily detected by fluorescence microscopy and further quantitatively analyzed. In addition, improved FISH analysis also allows simultaneous observation of multiple genes by labeling different sequence fragments with different fluorophores.. In plant systems, FISH analysis has been used in a variety of crops, including wheat, rye, cucumber and melon. Based on the information on Lifeasibles official website, Lifeasibles services cover every step of the FISH assay in the plant system:. • Probe designing and construction: aside from commercially available probes, Lifeasible also provides customized ...

Fluorescein is unionised at acidic pH and the fluorescence intensity changes with pH [19, 20]. As expected, our results clearly demonstrate this effect on fluorescence levels using a FRET pair and a quencher pair with FAM. By use of the ATTO495-ATTO647N FRET pair for Hoogsteen-based parallel triplex formation, a robust and reliably LightCycler method was established. This novel FRET pair is well-suited for Tm and ΔTm determinations over a broad pH range of parallel triplex formations.. Furthermore, this FRET pair clearly demonstrates the pH independence from pH 5.5 to 7.5 of antiparallel duplex Tm determinations in contrast to the pH dependent Tm determination of parallel triplex formation from pH 4.5 to 6.0. An interesting feature of this is the negative correlation between pH and fluorescence intensity as pH increases from 4.5 to 6.0 for parallel triplex formation (Figure 3c). This is in concordance with the expected lower efficacy of parallel triplex formation due to the lack of protonated ...

Widefield fluorescent image of epithelial cells (nuclei stained with DAPI, yellow; and filamentous actin stained with Alexa Fluor 488 phalloidin, magenta )

Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes inside living cells, resulting in brighter reporters than fluorescent proteins. Intracellular labeling using these techniques requires cell-permeable fluorescent ligands, however, limiting utility to a small number of classic fluorophores. Here we describe a simple structural modification that improves the brightness and photostability of dyes while preserving spectral properties and cell permeability. Inspired by molecular modeling, we replaced the N,N-dimethylamino substituents in tetramethylrhodamine with four-membered azetidine rings. This addition of two carbon atoms doubles the quantum efficiency and improves the photon yield of the dye in applications ranging from in vitro single-molecule measurements to super-resolution imaging. The novel substitution is generalizable, yielding a palette of chemical dyes ...

Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...

Glutathione (GSH) plays crucial roles in various biological functions, the level alterations of which have been linked to varieties of diseases. Herein, we for the first time expanded the application of oxidase-like property of MnO2 nanosheet (MnO2 NS) to fluorescent substrates of peroxidase. Different from previously reported fluorescent quenching phenomena, we found that MnO2 NS could not only largely quench the fluorescence of highly fluorescent Scopoletin (SC) but also surprisingly enhance t

On the substrate carrying a sub-wavelength grating covered with a thin metal layer, a fluorescent dye-labeled cell was observed by fluorescence microscope. The fluorescence intensity was more than 20 times greater than that on an optically flat glass substrate. Such a great fluorescence enhancement from labeled cells bound to the grating substrate was due to the excitation by grating coupled surface plasmon resonance. The application of a grating substrate to two-dimensional detection and fluorescence microscopy appears to offer a promising method of taking highly sensitive fluorescence images.. ©2008 Optical Society of America. Full Article , PDF Article ...

These can be very useful. Most allow you to plot out the excitation and emission curves of a number of fluorescent dyes. Some allow the user to add filter sets and excitation sources to see how well they work with the dyes being considered. Noteworthy examples include: an extensive database of single and 2-photon dye spectra assembled here at the University of Arizona (Utzinger & Boswell), Thermo-Fishers Fluorescence spectraviewer, Semrocks searchlight, Fluorophores.org, Chromas spectra viewer, Biolegends viewer, Leicas Fluoscout, Omega Opticals curvomatic, and Zeiss Fluorescence Dye and Filter Database ...

The boron complexes of dipyrromethene (BODIPY®) comprise a class of fluorescent dyes that can be used in medical and biological studies. Fluorescent molecular labeling can be obtained by conjugation, or linking fluorescent dyes to certain proteins and peptides, in order to detect interactions, conformational changes and localization of specific peptides and parts of a protein. BODIPY molecules are relatively insensitive to the polarity and pH of their environment and are very stable to physiological conditions. The BODIPY core is strong enough to withstand a series of chemical reactions, and even the smallest changes to their structures enable the activation of their fluorescent properties. Herein, we have successfully synthesized N, N-difluoroboryl-2,8-diethyl-1,3,7,9-tetramethyl-5-(4-isothiocyanatophenyl)-dipyrromethene, BODIPY derivative that is used for conjugation to proteins. Alternative pathway of synthesis is described with regard to literature. During the synthesis, a fluorescent ...

Coupling nanomaterials with biomolecular recognition events represents a new direction in nanotechnology toward the development of novel molecular diagnostic tools. Here a graphene oxide (GO)-based multicolor fluorescent DNA nanoprobe that allows rapid, sensitive, and selective detection of DNA targets in homogeneous solution by exploiting interactions between GO and DNA molecules is reported. Because of the extraordinarily high quenching efficiency of GO, the fluorescent ssDNA probe exhibits minimal background fluorescence, while strong emission is observed when it forms a double helix with the specific targets, leading to a high signal-to-background ratio. Importantly, the large planar surface of GO allows simultaneous quenching of multiple DNA probes labeled with different dyes, leading to a multicolor sensor for the detection of multiple DNA targets in the same solution. It is also demonstrated that this GO-based sensing platform is suitable for the detection of a range of analytes when ...

Single-molecule fluorescence spectroscopy and super-resolution microscopy are important elements of the ongoing technical revolution to reveal biochemical and cellular processes in unprecedented clarity and precision. Demands placed on the photophysical properties of the fluorophores are stringent a …

Fluorescent Dyes , Fluorescent Biopolymers , Beta-Amyloid (1-42), HiLyte Fluor 488-labeled, Human; This is a fluorescent (HiLyte Fluor 488)-labeled -Amyloid peptide, Abs/Em=503/528 nm. Hilyte 488 Fluor labeled A (1-42) has a brighter intensity than FAM-labeled A (1-42).; Solvent InformationFgure 1. Absoprtion and emission spectra of HiLyte Fluor 488 labeled β-Amyloid (1-42).Figure 2. Staining pattern of HiLyte Fluor 488 labeled Aβ (1-42) peptide on an AD (A) and a normal brain (B). Images courtesy of Dr. Jian-Ping Guo in Dr. Patrick L. McGeer s lab, University of British Columbia, Vancouver, Canada.; HiLyte Fluor 488-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA; Hilyte Fluor488-Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala-OH

In this study, we found a PyA-modified adenine cluster (A-cluster), a minimum fluorescent unit for significant emission wavelength changes, and investigated its photophysical and structural properties. The basic A-cluster unit was an adenine-pentad duplex containing stacked PyA pairs in the center aligned in 2015 Hot Articles in Organic and Biomolecular Chemistry

The rhodamine labeled f-actin did not appear in the composite image of the three different fluorophores due to the intensity of the fluorescence being too low to register. This could be due to photobleaching that had previously occurred in this specific area of the sample, or if the sample was not labeled adequately with enough fluorescent dye. Time-lapse data for all three fluorophores under the same condition revealed discrepancies in rate of decay and initial intensity for each fluorophore. A relative high initial intensity for DAPI labeled dsDNA can be explained by the relative high net local concentration of bright fluorophores. Each nucleus contains a high concentration of dsDNA, which when stained with DAPI, creates a large solid fluorescent region with overlapping fluorophores. This differs from both tubulin and f-actin, which are of tube like nature, and appear as porous regions of interest where background light can seep through and be analyzed, making the initial brightness in the ...

We recently finished our Ask the Expert discussion on Improving Live Cell Fluorescence Imaging. This week we had several interesting questions focused on combating the negative effects of imaging on cell health and viability including looking at media options as a possible solution.

Some APC-Cy7 conjugates show changes in their emission spectra with prolonged exposure to paraformaldehyde. For instruments equipped with a 561 nm Yellow-Green laser, a 585/15 nm filter is recommended for detection. When making decisions about which fluorochromes to use in your experiments, youll want to know their relative emission spectra. APC (Allophycocyanin) spectrum - APC (Allophycocyanin) is ... excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with emission at 605nm. APC-H7 conjugates provide greater stability in light and paraformaldehyde fixatives and have less spillover into the APC channel than APC-Cy7 conjugates. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. BV650 is a tandem fluorochrome ...

New techniques for biological optical imaging are of great interest for the detection and visualization of processes and disease in both clinical and research areas. One major advancement has been the use of far red and near infrared (NIR) light, as it has the ability to penetrate tissues deeper than other parts of the spectrum which are readily scatter and absorbed by the surroundings. In order to improve the signal to noise ratio and resolution of optical images, contrast agents are used. Fluorescent markers can be modified to attach to specific molecular targets, creating small molecular probes. These targets can be disease sites, or biological molecules which play a major role in processes such as tumor growth. It was our goal to create a new novel fluorescent probe, consisting of a cyanine based far red to NIR marker, and an n-hydroxysuccinimide (NHS) derivative to act as a linker, which could then bind with biological species containing primary amides such as proteins and antibodies, in this

The Quantus Fluorometer is a dual-channel fluorometer for your personal quantitation workflow. Designed to provide highly sensitive fluorescent detection when quantifying nucleic acids, the compact instrument is simple to operate.

A new fluorescent Zn2+ chemosensor (P1) based on a functionalized porphyrin was synthesized and characterized. P1 displayed dramatic ratiometric variations in absorption and fluorescent emission spectra upon exposure to Zn2+ due to the formation of a 1:1 Zn2+/P1 complex. The sensor also exhibited high selectivity and sensitivity toward Zn2+ over other common metal ions in the physiological pH range with a detection limit of 1.8 mM. The sensor showed fast response times and excellent reproducibility, thus confirming its potential applicability as a fluorescent sensor for Zn2+ sensing.

A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions). More generally they are covalently bonded to a macromolecule, serving as a marker (or dye, or tag, or reporter) for affine or bioactive reagents (antibodies, peptides, nucleic acids). Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, i.e., fluorescent imaging and spectroscopy. Fluorescein, by its amine reactive isothiocyanate derivative FITC, has been one of the most popular fluorophores. From antibody labeling, the applications have spread to ...

SH2 protein microarray assays of HA4 specificity(a) Fluorescence images of SH2 chips following incubation with fluorophore-labeled HA4. Each SH2 domain is spott

TY - JOUR. T1 - Highly photostable ketopyrrolyl-BODIPYs with red aggregation-induced emission characteristics for ultrafast wash-free mitochondria-targeted bioimaging. AU - Wu, Hao. AU - Guo, Xing. AU - Yu, Changjiang. AU - Wong, Wai Yeung. AU - Hao, Erhong. AU - Jiao, Lijuan. PY - 2020/5. Y1 - 2020/5. N2 - Subcellular organelle-specific probes, including mitochondria-targeted fluorescent probes, have attracted enormous research interests because they can monitor or visualize the morphology or biological activities of specific organelles and play an indispensable role in disease diagnosis. To follow the process, highly specific and photostable fluorescent probes are in demand. However, commercially available mitochondria probes normally suffer from poor photostability under laser irradiation and aggregation caused quenching (ACQ) in the aggregate state. In this work, two simple aggregation-induced emission(AIE)-active meso-2-ketopyrrolyl BODIPYs were developed via a convenient one-pot synthetic ...

Phosphorescent molecules are attractive complements to fluorescent compounds for bioimaging. Time-gated acquisition of the long-lived phosphorescence signals provides an effective means to eliminate unwanted background noises due to short-lived autofluorescence. We have previously investigated the molecular principles governing modulation of photoinduced electron transfer in phosphorescence zinc probes that were based on biscyclometalated Ir(III) complexes (Woo, H. et al. J. Am. Chem. Soc. 2013, 135, 4771-4787). The studies established that phosphorescence turn-on responses would be attainable for Ir(III) complexes with high triplet-state energies. This sets an upper limit to an emission wavelength, restricting the development of red- or near-IR-phosphorescence turn-on probes. To address this challenge, we designed and synthesized a new phosphorescent probe having an electron-deficient 2-(2-pyridyl)pyrazine diimine ligand tethering a di(2-picolyl)amine (DPA) zinc receptor. This ligand control ...

We offer Amine-Reactive Fluorophores; Bioconjugates; Biotin/Desthiobiotin; Buffers, Detergents; Caged Probes; Cell Analysis Kits; Cell stains; Click Chemistry; Enzyme Substrates; Fluorescent Mitochondrial Probes; Fluorescent Probes; Fluorescent/Non-Fluorescent Ion and pH Indicators; Fluorescent/Non-Fluorescent Lipids, Phospholipid Probes; Gel & Membrane Stains; Kits and Assays; Monomers; Natural Products and Their Fluorescent/None-Fluorescent Conjugates; Neurotransmitter receptors Probes; Nucleotides/Nucleosides; Protein Kinase Inhibitors (PKI); Quencher/Non-Fluorescent Probes; Reactive Probes and Related Products; Sensors; Steroids and Related Products

We offer Amine-Reactive Fluorophores; Bioconjugates; Biotin/Desthiobiotin; Buffers, Detergents; Caged Probes; Cell Analysis Kits; Cell stains; Click Chemistry; Enzyme Substrates; Fluorescent Mitochondrial Probes; Fluorescent Probes; Fluorescent/Non-Fluorescent Ion and pH Indicators; Fluorescent/Non-Fluorescent Lipids, Phospholipid Probes; Gel & Membrane Stains; Kits and Assays; Monomers; Natural Products and Their Fluorescent/None-Fluorescent Conjugates; Neurotransmitter receptors Probes; Nucleotides/Nucleosides; Protein Kinase Inhibitors (PKI); Quencher/Non-Fluorescent Probes; Reactive Probes and Related Products; Sensors; Steroids and Related Products

TY - JOUR. T1 - Capillary electrophoresis with laser-induced fluorescent detection of immunolabeled individual autophagy organelles isolated from liver tissue. AU - Muratore, Katherine A.. AU - Grundhofer, Heather M.. AU - Arriaga, Edgar A.. N1 - Funding Information: We thank Dr. M. Kyba from the Department of Pediatrics and Dr. D. Lowe from the Department of Physiology at the University of Minnesota for providing mice for the autophagy organelle isolation. We thank Dr. N. Mizushima from the University of Tokyo for the generous gift of ATG5(−/−) and ATG5(+/+) MEF cells, provided to us by Dr. D. Kim from the Department of Biochemistry, Molecular Biology, and Biophysics at the University of Minnesota. K.A.M., H.M.G., and E.A.A. thank the National Institutes of Health (Grants RO1-AG020866, T32-GM008700, and T32-AG029796). Publisher Copyright: © 2016 American Chemical Society.. PY - 2016/12/6. Y1 - 2016/12/6. N2 - Macroautophagy is a cellular degradation process responsible for the clearance of ...

Nanogen, Inc., developer of advanced diagnostic products, announced today its subsidiary, Epoch Biosciences, was issued Patent No. 6,951,930, Hybridization-Triggered Fluorescent Detection of Nucleic Acids by the U.S. Patent and Trademark Office. The 930 patent relates to latent fluorophore-minor groove binder oligonucleotide conjugates which fluoresce upon hybridization to a target. The conjugates may be used to detect nucleic acid targets. Source: ...

0039] 3-Diethylamino-6-methylfluorane, 3-diethylamino-6-methyl-7-anilinofluorane, 3-diethylamino-6-methyl-7-(o,p-dimethylanilino)fluorane, 3-diethylamino-6-methyl-7-chlorofluoran, 3-diethylamino-6-methyl-7-(m-trifluoromethylanilino) fluorane, 3-diethylamino-6-methyl-7-(o-chloroanilino) fluorane, 3-diethylamino-6-methyl-7-(p-chloroanilino) fluorane, 3-diethylamino-6-methyl-7-(o-fluoroanilino) fluorane, 3-diethylamino-6-methyl-7-(m-methylanilino) fluorane, 3-diethylamino-6-methyl-7-n-octylanilino fluorane, 3-diethylamino-6-methyl-7-n-octylamino fluorane, 3-diethylamino-6-methyl-7-benzylamino fluorane, 3-diethylamino-6-methyl-7-dibenzylamino fluorane; 3-diethylamino-6-chloro-7-methyl fluorane, 3-diethylamino-6-chloro-7-anilino fluorane, 3-diethylamino-6-chloro-7-p-methylanilino fluorane, 3-diethylamino-6-ethoxyethyl-7-anilino fluorane, 3-diethylamino-7-methyl fluorane, 3-diethylamino-7-chloro fluorane, 3-diethylamino-7-(m-trifluoromethylanilino) fluorane, 3-diethylamino-7-(o-chloroanilino) ...

Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules,[1] notably, to intracellular lysine residues and other amine sources. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Also, due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. CFSE is commonly confused with carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), although they are not strictly the same molecule; CFDA-SE, due to its acetate groups, is highly cell permeable, while CFSE is much less so. As CFDA-SE, which is non-fluorescent, enters the cytoplasm of cells, intracellular esterases remove the acetate groups and convert the molecule to the fluorescent ester. CFSE was originally developed as a fluorescent dye that could be used to stably label lymphocytes and track their migration within ...

CD11c, Alexa Fluor 700, clone: N418, eBioscience™ 25μg; Alexa Fluor 700 CD11c, Alexa Fluor 700, clone: N418, eBioscience™ Primary Antibodies CD11 to CD15

CD4, Alexa Fluor 700, clone: GK1.5, eBioscience™ 100μg; Alexa Fluor 700 CD4, Alexa Fluor 700, clone: GK1.5, eBioscience™ Primary Antibodies CD1 to CD5

Near-infrared (NIR, 700-900 nm) fluorescence imaging has been widely employed in preclinical research and clinical practice for noninvasive real-time tumor diagnosis and image-guided cancer surgery.1-6 This is driven by its unique advantages including deep light penetration (up to centimetre scale), negligible autofluorescence in the NIR spectrum, good safety without ionizing radiation, no requirement for complex infrastructure, and real-time visualization capabilities.7-10 This technology requires NIR fluorescent agents with superior chemical and photophysical properties. Among the various NIR fluorescent contrast agents investigated to date, organic NIR fluorescent nanoparticles (NPs) have been actively pursued in recent years due to their synthetic versatility and fewer cytotoxicity concerns.11-15 Moreover, each organic NP can encapsulate a large number of organic dyes,16 which exhibit better photostability than a single dye molecule. However, the confinement of a large number of organic dyes ...

Herein, we report a convenient approach to developing quantum dots (QDs)-based nanosensors for DNA and micro-RNA (miRNA) detection. The DNA-QDs conjugate was prepared by a ligand-exchange method. Thiollabeled ssDNA is directly attached to the QD surface, leading to highly water-dispersible nanoconjugates. The DNA-QDs conjugate has the advantages of the excellent optical properties of QDs and well-Controlled recognition properties of DNA and can be used as a nanoprobe to construct a nanosensor for nucleic acid detection. With the addition of a target nucleic acid sequence, the fluorescence intensity of QDs was quenched by an organic quencher (BHQ(2)) via Forster resonance energy transfer. This nanosensor can detect as low as 1 fM DNA and 10 fM miRNA. Moreover, the QDs-based nanosensor exhibited excellent selectivity. It not only can effectively distinguish single-base-mismatched and random nucleic sequences but also can recognize pre-miRNA and mature miRNA. Therefore, the nanosensor has high ...

We describe a laser-scanning two-photon fluorescence microscope that is capable of observing single molecules with excellent temporal resolution and three-dimensional spatial resolution. To demonstrate the capabilities of the instrument we present single-molecule fluorescence data obtained in several different scanning modes. In addition, a polarization-sensitive detection scheme can provide detailed three-dimensional information about the orientations of molecules in any of these scanning modes.. © 1999 Optical Society of America. Full Article , PDF Article ...

TY - JOUR. T1 - MegaStokes BODIPY-triazoles as environmentally sensitive turn-on fluorescent dyes. AU - Er, Jun Cheng. AU - Tang, Mui Kee. AU - Chia, Chee Geng. AU - Liew, Huimin. AU - Vendrell, Marc. AU - Chang, Young-Tae. PY - 2013. Y1 - 2013. N2 - A novel class of triazole-derivatized BODIPY compounds have been synthesized on solid-phase by employing mild reaction conditions based on the copper-catalyzed azide-alkyne cycloaddition. The resulting BODIPY-triazoles exhibited MegaStokes shifts (up to 160 nm) and remarkable environmentally sensitive quantum yield increments that asserted their potential as turn-on fluorescent sensors. Out of a library of 120 compounds, we identified BDC-9 as a fluorescent chemosensor with high sensitivity and remarkable species-selectivity towards human serum albumin. These results validate MegaStokes BODIPY dyes as new fluorophores for the development of environmentally sensitive fluorescent probes.. AB - A novel class of triazole-derivatized BODIPY compounds ...

TY - JOUR. T1 - Real time imaging of single fluorophores on moving actin with an epifluorescence microscope. AU - Sase, I.. AU - Miyata, H.. AU - Corrie, J. E T. AU - Craik, J. S.. AU - Kinosita, K.. PY - 1995. Y1 - 1995. N2 - Relatively simple modifications of an ordinary epifluorescence microscope have greatly reduced its background luminescence, allowing continuous and real time imaging of single fluorophores in an aqueous medium. Main modifications were changing the excitation light path and setting an aperture stop so that stray light does not scatter inside the microscope. A simple and accurate method using actin filaments is presented to establish the singularity of the observed fluorophores. It was possible, at the video rate of 30 frames/s, to image individual tetramethylrhodamine fluorophores bound to actin filaments sliding over heavy meromyosin. The successful imaging of moving fluorophores demonstrates that conventional microscopes may become a routine tool for studying dynamic ...

Vagal projecting (VP) neurons were localized by intraneural injections of fluorescent dyes or cholera toxin conjugated horseradish peroxidase (CT-HRP) or by intraperitoneal injection of fluorescent dyes. Spinal projecting (SP) neurons were localized by injecting CT-HRP or contrasting dyes into the C4/C5 cord segments. No doubly labelled neurons were seen in the three nuclei known to project to both vagus nerve and spinal cord, viz., dorsal nucleus of the vagus (DNV), nucleus ambiguous complex (NAc) and the intermediate region (NI) between DNV and NAc. VP and SP neurons intermingled in the caudal parts of the NAc and DNV. In the middle part of the NAc, VP neurons congregated mostly dorsal to the SP neurons. In the rostral extremity of the NAc, SP neurons were rarely encountered. No SP neurons were seen in the rostral end of the DNV. In contradistinction to the few VP neurons in the NI, there were many SP neurons in this region. The ratios of VP to SP neurons in DNV were on the average 20 to 1 and ...

Total internal reflection fluorescence microscopy (TIRFM) is an elegant optical technique utilized to observe single molecule fluorescence at surfaces and interfaces. This section is an index to our discussions, references, and interactive Java tutorials that describe TIRFM.

Shop a large selection of Protein Labelling Reagents products and learn more about Molecular Probes™ Alexa Fluor™ 750 NHS Ester (Succinimidyl Ester) 3 x 100μg

The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging ...

The decrease in fluorescence of conjugated polyenic acyl chains is used as a sensitive assay for lipid peroxidation. The fatty acid cis-trans-trans-cis-9,11,13,15-octadecatetraenoic acid (cis-parinaric acid) is introduced into liposomal membranes as free fatty acid or, by using the PC specific transfer protein from bovine liver, as 1-palmitoyl-2-cis-parinaroyl-sn-glycero-3-phosphocholine. The peroxidation process ... read more as monitored by the decrease in fluorescence intensity is compared with other peroxidation assay systems. Applications of the new assay system are discussed. show less ...

Fluorescence Resonance Energy Transfer (FRET) is an energy transfer process between the fluorescent dye molecules [1]. Due to its high sensitivity to the change of distance between the dyes, it has received great interest for the study of biological systems [2]. FRET technique has a spatial resolution of sub-nanometer, and can be used as a probe of inter- and intra-molecular dynamics of biological macromolecules such as protein conformational change, enzyme-substrate reaction, and RNA folding [3][4]. Determination of FRET efficiency in ensemble measurement is, however, not straightforward due to the difficulty in distinguishing FRET pairs from unpaired dyes or the dyes not properly labeled to the bio-molecule in question that might give huge background [5]. By contrast, single-molecule detection provides a method that is free from this obstacle as it can only collect the signal from one single FRET pair. Another advantage of this technique is that it allows monitoring the structural- or ...

PCR-based DNA amplification and presumptive detection of Escherichia coli O157:H7 with an internal fluorogenic probe and the 5 nuclease (TaqMan) assay

We demonstrate the use of fluorescent molecular rotors as probes for detecting biomolecular interactions, specifically peptide-protein interactions. Molecular rotors undergo twisted intramolecular charge transfer upon irradiation, relax via the nonradiative torsional relaxation pathway, and have been typically used as viscosity probes. Their utility as a tool for detecting specific biomolecular interactions has not been explored. Using the well characterized p53-Mdm2 interaction as a model system, we designed a 9-(2-carboxy-2-cyanovinyl) julolidine-based p53 peptide reporter, JP1-R, which fluoresces conditionally only upon Mdm2 binding. The reporter was used in a rapid, homogeneous assay to screen a fragment library for antagonists of the p53-Mdm2 interaction, and several inhibitors were identified. Subsequent validation of these hits using established secondary assays suggests increased sensitivity afforded by JP1-R. The fluorescence of molecular rotors contingent upon target binding makes them ...

TY - JOUR. T1 - Nanosecond fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy to localize the protein interactions in a single living cell. AU - Elangovan, M.. AU - Day, R. N.. AU - Periasamy, A.. PY - 2002/2/18. Y1 - 2002/2/18. N2 - Visualizing and quantifying protein-protein interactions is a recent trend in biomedical imaging. The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes such as green fluorescent proteins, allow fluorescence resonance energy transfer (FRET) to be used to study protein interactions in living specimens. Intensity-based FRET microscopy is limited by spectral bleed-through and fluorophore concentration. Fluorescence lifetime imaging (FLIM) microscopy and lifetime measurements are independent of change in fluorophore concentration or excitation intensity, and the combination of FRET and FLIM provides high spatial (nanometre) and temporal (nanoseconds) resolution. Because only the donor ...

Fomina M. V., Nikiforov A. S., Gromov S. P. Modern approaches to the synthesis and prospects for the use of cyanine dyes containing functional groups in the n-substituents // Russian Chemical Reviews. - 2016. - Vol. 85, no. 7. - P. 684-699. Methods for the synthesis of mono-, tri-, penta- and heptamethine cyanine dyes containing functional groups in N-substituents are considered. Approaches suitable for the preparation of both symmetrical and unsymmetrical cyanine dyes are presented. Examples of the practical use of this type of cyanine dyes as fluorescent labels and probes in biology and medicine, as well as of components of photoactive supramolecular structures, photosensitizers and photo- and electroluminescent materials are given. The bibliography includes 106 references. [ DOI ...

We prepared silica NPs doped with two far-red absorption cyanine dyes, FR670 and Cy5, using the microemulsion method. At these wavelengths there is less autofluorescence from biological molecules, solvents and substrates. The effects of changing surfactant, pH and dye loading on NP morphology and colloidal stability were investigated using transmission electron microscopy and photo correlation spectroscopy. We found that the incorporation of Cy5 and FR670 was only possible using negatively charged surfactants. We propose a method for the facile incorporation of cyanine dye based on the electrostatic repulsion between negatively charged dye molecules and the negatively charged surfactant, present at the water oil interface, which drives the dye into the silica NP forming inside the water phase. The absorption, fluorescence and quantum efficiency of the NPs were also measured and compared against free dye labels. The optimal fluorescences for FR670 and Cy5 dye doped NPs, were 178 and 83 times brighter

SERVA TBE Clear G Agarose Tablets are fast-solving tablets which not only contain TBE buffer but as well the non-carcinogenic, sensitive fluorescence dye DNA Stain Clear G.Just add water, solve the agarose and your agarose gel is ready! ♦ For analytical and preparative DNA and RNA gel electrophoresis and blotting (100 bp - ≥30 kb)♦ Fast-solving agarose for gels with high clarity and low background♦ Optimized mixture for high resolution of sharp bands with high sensitivity♦ No clumping because separately packed in blister pack

TY - JOUR. T1 - Tuning fluorescent molecules by inclusion in a metal-organic framework: an experimental and computational study. AU - Yan, Dongpeng. AU - Lloyd, Gareth O. AU - Delori, Amit. AU - Jones, William. AU - Duan, Xue. PY - 2012/12. Y1 - 2012/12. N2 - Fluorescent molecules included within a metalorganic framework (MOF) offer a new opportunity to develop luminescent hostguest materials. Two linear p-conjugated fluorescent molecules, 1,4-bis-p-cyanostyrylbenzene (bpcb) and 1,4-bis(5-phenyloxazol-2-yl)benzene (bpob), incorporated into MOF-5 systems are reported. The as-obtained bpcb@MOF-5 and bpob@MOF-5 displayed tunable blue fluorescent properties (emission wavelength, fluorescent lifetime, and quantum yield) compared with the pure guest molecule and host matrix. Moreover, bpcb@MOF-5 and bpob@MOF-5 thin films were fabricated by the solvent-evaporation method. The MOF-based thin films exhibit well-defined polarized blue emission with a fluorescent anisotropy of approximately 0.15, ...

Structural characterization by super-resolution microscopy has become increasingly widespread, particularly in the biological community. The technique is powerful because it can produce real-space images with resolutions of tens of nanometers, while sample preparation is relatively non-invasive. Previous studies have applied these techniques to important scientific problems in the life sciences, but relatively little work has explored the attainable limit of resolution using samples of known structure. In this work, we apply photo-activated localization microscopy (PALM) to polymer films that have been nanopatterned using electron-beam lithography. Trace amounts of a rhodamine spiroamide dye are dispersed into nanostructured poly(methyl methacrylate), and UV-induced switching of the fluorophores enables nanoscale localization of single molecules to generate a final composite super-resolution image. Features as small as 25 nm half-pitch are clearly resolvable.. We also explore the effect of ...

As a high-resolution wide-field near-surface microscopy, total internal reflection fluorescence microscopy (TIRFM) has been widely applied for the study of biomolecules. Unlike those costly, sample consuming and time consuming traditional detection assays, the application of TIRFM enable the direct quantification of biomolecules in a sample pretreatment and enrichment free fashion. Taking advantages of the TIRFM imaging system, in this thesis we have applied the TIRFM imaging system to directly quantify the content of different cancer associated biomarkers. Four different detection approaches for direct cancer biomarkers quantification with the aid of TIRFM were herein presented respectively. In Chapter 2, a direct quantification of nasopharyngeal carcinoma associated miRNAs was described. In the assay, five different miRNAs were chosen as the target analytes, which hybridized with the synthetic complementary LNA, probe in solution. The duplex was labeled with intercalating fluorescence dye YOYO-1 and

Find Molecular Probes® fluorescent labels for multiplexed super-resolution microscopy (SRM) applications, including STORM, STED, SIM, and two-photon microscopy.

Alexa Fluor 350/488/594 Filter Set (Semrock Brightline) *mounted* *Zeiss Axio AF from Molecular Probes (Invitrogen),Alexa Fluor 350/488/594 Filter Set (Semrock Brightline) *mounted* *Zeiss Axio AF,biological,biology supply,biology supplies,biology product

A super-resolution platform for correlative live single-molecule imaging and STED microscopy. Super-resolution microscopy offers tremendous opportunities to unravel the complex and dynamic architecture of living cells. However, current super-resolution microscopes are well suited for revealing protein distributions or cell morphology, but not both. We present a super-resolution platform that permits correlative single-molecule imaging and stimulated emission depletion microscopy in live cells. It gives nanoscale access to the positions and movements of synaptic proteins within the morphological context of growth cones and dendritic spines.. ...

A novel fluorescence sensing system for branched-chain amino acids (BCAAs)was developed based on engineered leucine/isoleucine/valine-binding proteins (LIVBPs)conjugated with environmentally sensitive fluorescence probes. LIVBP was cloned fromEscherichia coli and Gln149Cys, Gly227Cys, and Gln254Cys mutants were generated bygenetic engineering. The mutant LIVBPs were then modified with environmentallysensitive fluorophores. Based on the fluorescence intensity change observed upon thebinding of the ligands, the MIANS-conjugated Gln149Cys mutant (Gln149Cys-M) showedthe highest and most sensitive response. The BCAAs Leu, Ile, and Val can each bemonitored at the sub-micromolar level using Gln149Cys-M. Measurements were alsocarried out on a mixture of BCAFAs and revealed that Gln149Cys-M-based measurementis not significantly affected by the change in the molar ratio of Leu, Ile and Val in thesample. Its high sensitivity and group-specific molecular recognition ability make the newsensing system ideally suited

Fluorescent 2-deoxynucleotides containing a protecting group at the 3-O-position are reversible terminators enabling array-based DNA sequencing by synthesis (SBS) approaches. Herein, we describe the synthesis of a new family of 3-OH unprotected cleavable fluorescent 2-deoxynucleotides and their evaluation as reversible terminators for high-throughput DNA SBS strategies. In this first version, all four modified nucleotides bearing a cleavable disulfide Alexa Fluor(R) 594 dye were assayed for their ability to act as a reversible stop for the incorporation of the next labeled base. Their use in SBS leaded to a signal-no signal output after successive addition of each labeled nucleotide during the sequencing process (binary read-out). Solid-phase immobilized synthetic DNA target sequences were used to optimize the method that has been applied to DNA polymerized colonies or clusters obtained by in situ solid-phase amplification of fragments of genomic DNA templates Turcatti, G.; Romieu, A.; Fedurco, M.;

Anti-Rabbit Fc Alexa Fluor® 594 secondary antibody validated for ICC/IF, IHC-Fr, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 9 publications. Other Alexa Fluor® 594 secondaries…

Anti-Rabbit Fc Alexa Fluor® 568 secondary antibody validated for ICC/IF, IHC-Fr, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Other Alexa Fluor® 568 secondaries available.

Novel fluorescent tools such as green fluorescent protein analogs and Fluorogen Activating Proteins (FAPs) are useful in biological imaging to track protein dynamics in real-time with low fluorescence background. evolution experiments of both VH-VL M8 and M8VL, led us to rationally design tandem, covalent homodimers of M8VL domains joined by a flexible linker that have a higher affinity for DIR and great quantum yield. The introduction of fluorescent systems has revolutionized mobile imaging and molecular biology, as well as the electricity of encoded fluorescent proteins, such as for example green fluorescent proteins (GFP), for the recognition of particular proteins appealing can be well recorded (1). Theres a dependence on extra still, well-characterized tools offering real-time, high signal-to-noise fluorescence and demonstrate high fluorescence quantum produce (?f), photo-stability, and a wide spectral range. Fluorogen Activating Protein (FAPs) are section of book, immunoglobulin-based, ...

TY - CHAP. T1 - 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. AU - Novopashina, D. S.. AU - Semikolenova, O. A.. AU - Venyaminova, A. G.. PY - 2020/1/1. Y1 - 2020/1/1. N2 - Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.. AB - Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and ...

Scattering of shorter-wavelength visible light limits the fluorescence imaging depth of thick specimens such as whole organs. In this study, we report the use of four newly synthesized near-infrared and far-red fluorescence probes (excitation/emission, in nm: 644/670; 683/707; 786/814; 824/834) to image tumor cells in the subpleural vasculature of the intact rat lungs. Transpelural imaging of tumor cells labeled with long-wavelength probes and expressing green fluorescent protein (GFP; excitation/emission 488/507 nm) was done in the intact rat lung after perfusate administration or intravenous injection. Our results show that the average optimum imaging depth for the long-wavelength probes is higher (|mml:math alttext=$27.8 \pm 0.7$ id=E1 xmlns:mml=http://www.w3.org/1998/Math/MathML||mml:mn|27.8|/mml:mn||mml:mo|±|/mml:mo||mml:mn|0.7 |/mml:mn||/mml:math||mml:math alttext=$\mu$ id=E2 xmlns:mml=http://www.w3.org/1998/Math/MathML||mml:mi|μ|/mml:mi||/mml:math|m) than for GFP (|mml:math alttext=

anti-CD79A, Alexa Fluor 647, Clone: SPM549, Novus Biologicals 100 Tests; Alexa Fluor 647 Life Sciences:Antibodies:Primary Antibodies:Flow Cytometry (Flow)

anti-Mouse CYBB/NOX2, Alexa Fluor 405, Clone: NL7, Novus Biologicals 100 Tests; Alexa Fluor 405 Life Sciences:Antibodies:Primary Antibodies:Flow Cytometry (Flow)