Serum samples collected from breeder chickens ranging in age from 1 day to 55 weeks were tested for CAA antibodies by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent antibody (IFA) test. The relationship of ELISA to IFA test was determined. The sensitivity of the ELISA relative to the IFA test was 82.64%, and the specificity of the ELISA relative to the IFA test was 56.25%. Agreement between the ELISA and the IFA test was highly significant (Kappa = 0.74, Z = 5.78). We concluded that the ELISA is as good as the IFA test for detecting CAA antibody in sera from chickens.
IFA kits allow you to test for autoimmune, bacterial, and viral diseases, as well as parasites. Find quality kits when you shop online at RapidTest.
IFA kits allow you to test for autoimmune, bacterial, and viral diseases, as well as parasites. Find quality kits when you shop online at RapidTest.
Video articles in JoVE about transforming growth factors include In vivo-like Organotypic Murine Retinal Wholemount Culture.
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Define indirect fluorescent antibody. indirect fluorescent antibody synonyms, indirect fluorescent antibody pronunciation, indirect fluorescent antibody translation, English dictionary definition of indirect fluorescent antibody. adj. 1. Diverging from a direct course; roundabout. 2. a. Not proceeding straight to the point or object. b. Not forthright and candid; devious. 3.
Complications. The most severe complication is glaucoma secondary to anterior uveitis.(the cause of 10% of the cases of blindness in our survey ).At present, all treatments are ineffective. The other observed complications are KCS, corneal pigmentation, iris atrophy, cataract, retinal detachment, panophthalmitis and phtisis bulbi. Parasitological techniques Leishmania amastigote can be observed from lymph nodes, bone marrow smears or from ocular tissue. The sensitivity of the technique is low except for smears from granulomas which always reveal a large number of parasites. Cultures and inoculation take too long for practical purposes.. Serological tests. The Indirect Immunofluorescent Antibody Test (IFAT) and Enzyme Linked Immunosorbent Assay (ELISA) are techniques used worldwide. The Direct Agglutination Test is also a good tool under field conditions. If available, the Western Blot technique is more sensitive and suitable for diagnosis of ocular forms.. Molecular techniques. If serological ...
Species, Research Grants, Research Topics, Publications, Genomes and Genes, Scientific Experts about indirect fluorescent antibody technique
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The expression of variable antigen types (VATs) was determined among dividing populations of T. congolense growing in vivo in rabbit chancres and in vitro on bovine aorta endothelial cell monolayers. Experiments were performed in which a single metacyclic VAT (M-VAT) was deleted from a cultured metacyclic population by neutralisation with a monoclonal antibody and complement. Subsequent expression of the deleted M-VAT and two unrelated M-VATs was determined by an indirect immunofluorescent antibody test. The deleted M-VAT was re-expressed both in vivo and in vitro and the proportions of unrelated M-VATs were not markedly affected by the neutralisation of this single M-VAT. In addition, an overall similarity was observed between M-VAT expression and re-expression in vivo and in vitro. ...
Objective. The aim of the survey was to evaluate Toxoplasma gondii seroprevalence in small ruminants and possible risk factors associated with the infection. Materials and methods. Sera from 474 goats and 502 sheep reared on 42 farms in northern Italy were collected and tested for IgG antibodies to T. gondii by IFAT (indirect immunofluorescence antibody test). To identify risk factors, a binary logistic regression analysis of the variables was performed. An audit form about farm management was used. Results. Antibodies to T. gondii were found in 96.6% of goat farms and in 87.5% of sheep farms; 41.7% goats and 59.3% sheep resulted positive. Seroprevalence was significantly higher in sheep than in goats. Seroprevalence values were similar in goats from eastern and western areas, whereas goats from the southern area were at lower risk of infection. Saanen goats presented the lowest seroprevalence (30.7 %), whereas cross-breed exhibited the highest rate (48.7%). Goats from farms housing both sheep ...
A range of monoclonal antibodies (AquaMab-P) to detect specific fish and shrimp pathogens is available. These are supplied with a standard protocol to apply these in immunohistochemistry (IHC). These products can also be used in the indirect immunofluorescence antibody test (IFAT). ...
Dear Editor, We have read the letter by Bossuyt X. and Fieuws S. entitled "Detection of anti-nuclear antibodies, added-value of solid phase assay?" with great interest (1). In this letter the authors described a comparison between anti-nuclear antibodies (ANA) performed by indirect immunofluorescent assay(IIFA) and by an automated method (fluoroenzymeimmunoassay; EliA CTD screen, Thermo Fisher) using samples obtained from patients with systemic lupus erythematosus (SLE), systemic sclerosis (SSc),Sj?grens syndrome (SS) and healthy controls. The authors concluded that the favorable method for ANA detection is disease-dependent and that combining IIFA with solid phase assay can increase the diagnostic accuracy. These points, raised by Bossuyt X. and Fieuws S., may be regarded in the perspective of the international recommendations for ANA detection that we have recently published (2). Indeed, our recommendations support the use of IIFA as well as alternative methods (such as EliA) to determine ...
Objective. To analyze the clinical value of anti-DFS70 antibodies in a cohort of patients undergoing routine antinuclear antibodies (ANAs) testing. Methods. Sera with a dense fine speckled (DFS) indirect immunofluorescence (IIF) pattern from 100 consecutive patients and 100 patients with other IIF patterns were tested for anti-DFS70 antibodies by a novel chemiluminescence immunoassay (CIA) and for ANA by ANA Screen ELISA (both INOVA). Results. Among the 100 patients with a DFS IIF pattern, 91% were anti-DFS70 positive by CIA compared to 3% in the comparator group . The CIA and IIF titers of anti-DFS antibodies were highly correlated (rho = 0.89). ANA by ELISA was positive in 35% of patients with the DFS IIF pattern as compared to 67% of patients with other patterns . Only 12.0% of patients with DFS pattern and 13.4% with DFS pattern and anti-DFS70 antibodies detected by CIA had systemic autoimmune rheumatic disease (SARD). Only 5/91 (5.5%) patients with anti-DFS70 antibodies had SARD and their sera
The results of antinuclear antibody tests using the indirect immunofluorescence technique may be reported as a description of the pattern and the intensity of fluorescence obtained at a certain dilution. If quantitative results are required titration is necessary. Such titrations may vary greatly between different laboratories. The present study involving 26 laboratories shows an improvement of interlaboratory comparability for the homogeneous fluorescence pattern when a common reference serum is used. Cultured cells as substrate appear to give better quantitative agreement than rat liver sections. National reference sera should be standardised in items of the appropriate WHO reference preparation. ...
Robinson, C. and Guille, M. 1999. Immunohistochemistry of Xenopus embryos. Methods Mol. Biol. 127: 89-97. Sive, H.L., Grainger, R.M., and Harland, R.M. 2000. Early development of Xenopus laevis: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. Becker, B.E and Gard, D.L. 2006. Visualization of the cytoskeleton in Xenopus oocytes and eggs by confocal immunofluorescence microscopy. Methods Mol. Biol. 322: 69-86. ...
BioAssay record AID 332145 submitted by ChEMBL: Induction of cellular microtubule disrupting activity in rat A10 cells at 1 ug/ml after 24 hrs by indirect immunofluorescence technique.
Characterization of iHeps in vitro. a Immunofluorescence analysis of ALB and AAT in iHeps. More than 90 % of iHeps efficiently expressed both ALB and AAT at da
Various methods have been employed in the epidemiological assessment of malaria. In recent years, new serological techniques have suppemented the measurement of spleen rate and parasite rate. Since clasical malariometric indices such as parasite rate and annual parasite incidence were insufficient and not adequately sensitive to assess the progress of control measures, serological methids have been employed. The indirect fluorescent antibody test (IFA) was the serological test employed in this laboratory. Thick blood films of simian malaria parasites, plasmidium cynomolgi bastianellii and p.fieldi, obtained from infected rhesuns monkeys (Macaca mulatta) were used as antigens. The IFA test was shown to be useful to study the antibody levlels of blood donors from different areas in Sri Lanka, to observe the production and persistence of malarial antibodies in man and rhesus monkeys and for epidemiological assessment of malaria in Sri Lanka. The study conducte on 1050 blood donors revealed that ...
The first case of Q fever in Taiwan was reported in 1993. The disease is considered to be emerging in Taiwan, but the route of transmission has remained unclear. The annual number of confirmed Q fever cases has been increasing up to more than 100 cases since 2005, comparing with less than 30 before 2003. The purpose of this study was to determine the seroprevalence and risk factors of Coxiella burnetii infection in veterinary-associated populations in southern Taiwan. A total of 228 serum samples of high risk individuals engaging in veterinary-related work or animal-farm work, were collected between March and June in 2007. The study individuals were interviewed by a structured questionnaire designed for Q fever investigation. Serum samples from different animal species were also obtained for Q fever analysis in the same study areas. Serological test was conducted by indirect immunofluorescence antibody assay (IFA). The result demonstrated the overall seroprevalence of Q fever was 26.3% in ...
As rabies still represents a major public threat with tens of thousands of deaths per year, particularly in developing countries, adequate surveillance based on rapid and reliable rabies diagnosis for both humans and animals is essential. Rabies diagnosis relies on highly sensitive and specific laboratory tests for detection of viral antigens. Among those tests, at present the immunofluorescence antibody test is the
a total of 1,554 dogs from 5 countries on 3 continents were tested for antibodies to neospora caninum using an indirect fluorescent antibody test. in australia, overall, 42/451 (9%, 95% confidence interval [ci] 6-12%) dogs were seropositive (melbourne 11/207 [5%, 95% ci 2-9%]; sydney 18/150 [12%, 95% ci 7-18%]; perth 13/94 [14%, 95% ci 8-22%]). antibodies to n. caninum were also detected in dogs in south america (uruguay [20%, 95% ci 16-24%, n = 414]) and sub-saharan africa (tanzania [22%, 95% c ...
Purpose and History During prostate advancement mesenchymal-epithelial connections regulate body organ development and differentiation. in situ hybridisation display screen and researched Decorin (Dcn) Semaphorin6D (Sema6D) SPARC/Osteonectin (SPARC) Sprouty1 (Spry-1) and Tsukushi (Tsku). Appearance in rat SYN-115 tissue was examined using wholemount in situ hybridisation (postnatal time (P) 0.5) and immunohistochemistry (embryonic time (E) E17.5 E19.5; …Read More. ...
Figure 2. Localization of PMCA in human corneal epithelium. A: Immunostaining with pan-PMCA antibody (5F10) revealed strong PMCA labeling in all layers of the corneal epithelium (CE). The staining was associated primarily with the plasma membranes. The stroma (S) was unstained. B: Control section incubated with nonimmune mouse IgG was unstained.. ...
Has anyone ever performed or have a descriptive protocol for retinal flat mounts with mouse or rat retinas? This is a new technique for me, as such would need a very detailed method ...
Nowoczesna diagnostyka laboratoryjna. Dwuetapowa diagnostyka boreliozy. Molekularna diagnostyka alergii. Testy na alergię z krwi. Przeciwciała onkoneuronalne. IIFT. ELISA. Blot.
FT. MYERS, Fla., Aug. 22, 2014 /PRNewswire/ -- NeoGenomics Announces Closing of Recent Equity Offering and Exercise in Full of Underwriters Over-Allotment...
Borna disease virus-1 (BoDV-1) was recently discovered as cause of severe and often fatal encephalitis in humans. BoDV-1 is known to cause neurological disease in horses and sheep mainly in South and Central Germany. The virus is maintained in bicolored white-toothed shrews (Crocidura leucodon). The incidence of infection and risk factors in humans are completely unresolved. Veterinarians may be disproportionally BoDV-1-exposed through contact to animals not recognized to be BoDV-1 infected. We conducted three serosurveys predominantly in endemic areas of South Germany for the presence of BoDV-1-reactive antibodies. Anonymized residual samples from two serosurveys of veterinarians (n = 736) with interview data on exposures and one serosurvey among blood donors (n = 373) were screened with an indirect immunofluorescence antibody test, followed by a newly developed immunoblot as confirmatory assay. One serum from a 55-59-year-old veterinarian who worked in an animal practice and as a meat ...
Background: Mycobacterium tuberculosis is a major cause of mortality and morbidity worldwide. Infection with this bacterium is known to induce the development of autoantibodies of which a few are also known to be diagnostic markers for some other diseases. Antineutrophil Cytoplasmic Antibodies (ANCAs) are among those autoantibodies used in clinical setting for diagnosing systemic vasculitic syndromes. Multiple studies investigated ANCA positivity in diseases other than small vessel vasculitis. Objective: This study was performed to determine the prevalence of ANCA in pulmonary tuberculosis (TB) which may lead to the false diagnosis of Wegeners granulomatosis (WG) or vice versa. Methods: In a case-control study, 32 consecutive smear positive pulmonary TB patients and 32 normal individuals were studied. All cases and controls were screened for ANCA by indirect immunofluorescent assay (IIF), and MPO and PR3 were also tested by ELISA. Results: A prenuclear pattern (PANCA) was detected in 25% of the cases
CASTILHO, Juliana Galera et al. Antigenic and genetic characterization of the first rabies virus isolated from the bat Eumops perotis in Brazil. Rev. Inst. Med. trop. S. Paulo [online]. 2008, vol.50, n.2, pp.95-99. ISSN 1678-9946. http://dx.doi.org/10.1590/S0036-46652008000200006.. Although the main transmitters of rabies in Brazil are dogs and vampire bats, the role of other species such as insectivorous and frugivorous bats deserves special attention, as the rabies virus has been isolated from 36 bat species. This study describes the first isolation of the rabies virus from the insectivorous bat Eumops perotis. The infected animal was found in the city of Ribeirão Preto, São Paulo. The virus was identified by immunofluorescence antibody test (FAT) in central nervous system (CNS) samples, and the isolation was carried out in N2A cell culture and adult mice. The sample was submitted to antigenic typing using a panel of monoclonal antibodies (CDC/Atlanta/USA). The DNA sequence of the ...
TY - CONF. T1 - Proteome analysis of serum-cultured human corneal fibroblasts. AU - Karring, Henrik AU - Thøgersen, Ida B. AU - Klintworth, Gordon K. AU - Enghild, Jan J. AU - Møller-Pedersen, Torben. PY - 2004. Y1 - 2004. M3 - Poster. Y2 - 8 November 2004 through 12 November 2004. ER - ...
Single-cell analysis reveals aspects of cellular physiology not evident from population-based studies, particularly in the case of highly multiplexed methods such as mass cytometry (CyTOF) able to correlate the levels of multiple signaling, differentiation and cell fate markers. Immunofluorescence (IF) microscopy adds information on cell morphology and the microenvironment that are not obtained using flow-based techniques, but the multiplicity of conventional IF is limited. This has motivated development of imaging methods that require specialized instrumentation, exotic reagents or proprietary protocols that are difficult to reproduce in most laboratories. Here we report a public-domain method for achieving high multiplicity single-cell IF using cyclic immunofluorescence (CycIF), a simple and versatile procedure in which four-color staining alternates with chemical inactivation of fluorophores to progressively build a multichannel image. Because CycIF uses standard reagents and instrumentation ...
There are many home-made serological assays developed by different laboratories. The most frequently used test formats are the indirect fluorescent antibody test (IFA) using frozen sections of adult worms, and immunoenzyme assays (mostly ELISAs) with crude or recombinant antigens from eggs or adult worms.. Diagnostic strategies ...
Semantic Scholar extracted view of High resolution detection of DNA-RNA hybrids in situ by indirect immunofluorescence. by George T. Rudkin et al.
... is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope. - Immunofluorescence - AbVideo™ - Support - Abnova
This video demonstrates three types of morphometric analyses of the retina, which include measuring the inner nuclear layer thickness,...
Immunofluorescence staining of epidermal LCs in situ. Groups of mice (n = 2) received a single 100 μl injection intraperitoneally of 40 μg anti-α6 integrin
A serum indirect fluorescent antibody test (IFAT) was compared with a Western blot (WB) and a modified Western blot (mWB) for diagnosis of equine protozoal myeloencephalitis (EPM). Using receiver-operating characteristic (ROC) analysis, the area under the curve of the IFAT was greater than the area under the curves of the WB and the mWB (P=0.025 and P=0.044, respectively). There was no statistically significant difference between the areas under the curves of the WBs (P,0.05). On the basis of Show moreA serum indirect fluorescent antibody test (IFAT) was compared with a Western blot (WB) and a modified Western blot (mWB) for diagnosis of equine protozoal myeloencephalitis (EPM). Using receiver-operating characteristic (ROC) analysis, the area under the curve of the IFAT was greater than the area under the curves of the WB and the mWB (P=0.025 and P=0.044, respectively). There was no statistically significant difference between the areas under the curves of the WBs (P,0.05). On the basis of an ...
The diagnostic value of the detection of immunoglobulin G (IgG) and IgM by Bartonella henselae-based indirect fluorescence assay (IFA) and enzyme-linked immunoassay (EIA) for the diagnosis of cat scratch disease (CSD) was evaluated. The IFA was performed either with B. henselae that was cocultivated for a few hours with Vero cells or with noncocultivated B. henselae as the antigen. Additionally, the performance of a Bartonella PCR hybridization assay based on the 16S rRNA gene was determined and compared with those of the serologic assays. The study group consisted of 45 patients suspected of suffering from CSD by fulfilling one or more of the classical criteria. The specificities of the immunoassays were set at , or = 95% by analysis of sera from 60 healthy blood donors. It is shown that the sensitivities of the IgG assays are very low (40.9% for the IFA with noncocultivated B. henselae as antigen) and that those of the IgM assays are higher (71.4% for the EIA) for patients who fulfilled two or ...
Looking for Antinuclear Antibody Test? Find out information about Antinuclear Antibody Test. antinuclear antibody Explanation of Antinuclear Antibody Test
Summary Fluorescent antibody technics were used to determine the localization and distribution of Schistosoma mansoni antigen in tissue cells, the presence of circulating antibody, and the sites of antibody production or in vivo antigen-antibody combination. The experiments were performed in mice after a primary infection with cercariae, after several challenges, and after antigen stimulation and challenge. Evidence of the presence of circulating antibody was first observed at 20 days in the inhibition fluorescent antibody test, at 25 days in the indirect fluorescent antibody test, at 42 days with the cercarial fluorescent antibody test, and at 47 days in the Cercarienhullen reaction. Sites of antibody production or in vivo antigen-antibody combination were observed in inflammatory cells of the portal tracts, especially those along the smaller arteries, and in granulomas and isolated cells in the parenchyma of the liver; in the perivascular tissue cells, isolated parenchymal cells and granulomas of the
Journal of Immunology Research is a peer-reviewed, Open Access journal that provides a platform for scientists and clinicians working in different areas of immunology and therapy. The journal publishes research articles, review articles, as well as clinical studies related to classical immunology, molecular immunology, clinical immunology, cancer immunology, transplantation immunology, immune pathology, immunodeficiency, autoimmune diseases, immune disorders, and immunotherapy.
This program is concerned with the quality of indirect immunofluorescence testing of sera for detection of circulating IgG anti-basement membrane zone and anti-intercellular (cell surface) antibodies. The participating physician or laboratory director receives five serum samples and must process these to determine the presence and titer of circulating antibodies using an indirect immunofluorescence technique with monkey esophagus as a substrate and other substrates if desired. The participant records the results including pattern and the titer. Each participant returns the recorded results to Beutner Labs, Inc. The results of all laboratories in the same testing event are number coded, compiled and summarized for quality review. Beutner Labs, Inc. provides this service in cooperation with the Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo. Following completion of specimen tests from Beutner Labs, Inc. participants ...
The contribution of autoimmune phenomena to dysfunction of hypophysis or hypothalamus is far to be unraveled and also the specific pathways of hypophysitis are poorly understood until now, mostly for the pediatric population. Primary hypophysitis is rare in children and often regarded as an autoimmune disorder, following the evidence of lymphoplasmacytic infiltration in the pituitary gland, detection of anti-pituitary antibodies (APA) and anti-hypotalamus antibodies (AHA) by indirect immunofluorescence on cryostatic sections of human or primate hypophysis and hypothalamus, and coexistence with other autoimmune disorders. The rarity of this condition and the lack of ad hoc studies make hard any assessment of the real incidence of hypophysitis in pediatric patients, and also the role of APA and AHA has been poorly investigated in children with idiopathic hypopituitarism. Potential target autoantigens studied in autoimmune hypophysitis have been various pituitary-specific factors, chaperone ...
Figure 4 Cytological analysis of electroporated OrgD3 YS-explants. Abbreviations: AFP: α-foeto-protein; Control non-electroporated YS-explants (A), maintained in organ culture, organise into a bubble-like structure that contains a compacted part, at the site adhering to the culture dish (asterisk). Erythroid cells (arrow), differentiated from explanted YS mesoderm, are located close to this adhering site (A). The bubble-like part harbours clusters similar to YS-blood islands (B: Enlargement of the square in A), which also contains erythroid cells (arrow). CD31+ cells are present in the both in the bubble-like structure and adhering site (asterisk) (C). Whereas in the adhering site, the nature of labelled cells (arrows) is unclear, CD31+ endothelial cells (D) are clearly present cells in the bubble. In electroporated YS explants, both without (E, F) or with (G, H) plasmid, the endoderm, revealed by AFP wholemount in situ hybridization, remains external, but does not cover the whole ...
We would also like to pass on our thanks  to all the visitors who popped in and met us on our exhibition stand at IFAT 2014 in Munich last month. The show ...
Tested in order to determine the specificity of antibodies that have produced a positive ANA pattern (homogeneous and speckled) on HEp-2 cells. Antigens included are: SSA/Ro60, SSB/La, Sm, nRNP, ...
ZTE is prepping for one of the biggest events in the mobile world in Berlin. This years IFA takes place September 4-9 and as always will gather all the...
The objectives of this study were to evaluate the accuracy of the indirect fluorescent antibody test (IFAT) using serum and cerebrospinal fluid (CSF) of horses naturally and experimentally infected with Sarcocystis neurona, to assess the correlation between serum and CSF titres, and to determine the effect of S. neurona vaccination on the diagnosis of infection. 110 horses with or without neurological disease submitted for necropsy to the California Animal Health and Food Safety Laboratory Show moreThe objectives of this study were to evaluate the accuracy of the indirect fluorescent antibody test (IFAT) using serum and cerebrospinal fluid (CSF) of horses naturally and experimentally infected with Sarcocystis neurona, to assess the correlation between serum and CSF titres, and to determine the effect of S. neurona vaccination on the diagnosis of infection. 110 horses with or without neurological disease submitted for necropsy to the California Animal Health and Food Safety Laboratory System, ...
TY - JOUR. T1 - Diagnosis of babesiosis. T2 - Evaluation of a serologic test for the detection of babesia microti antibody. AU - Krause, Peter J.. AU - Telford, Sam R.. AU - Ryan, Raymond. AU - Conrad, Patricia A. AU - Wilson, Marianna. AU - Thomford, John W.. AU - Spielman, Andrew. PY - 1994. Y1 - 1994. N2 - To assess the possibility of standardization of a commonly used indirect immunofluorescent antibody (IFA) test for detection of Babesia microti antibody in human sera, the results from four reference laboratories were compared. Patients with babesiosis from southern New England (n = 25) and subjects with no history ofbabesiosis from southern New England (n = 55) and Iceland (n = 50) were enrolled in the study. Anti-Babesia antibody titers were determined in a blinded fashion by IFA test. The range of test results in the four laboratories was 88%-96% sensitivity, 90%-100% specificity, 69%-100% positive predictive value, and 96%-99% negative predictive value. Interlaboratory and ...