Species, Research Grants, Research Topics, Publications, Genomes and Genes, Scientific Experts about indirect fluorescent antibody technique
Cloyd, M W.; Bolognesi, D P.; and Bigner, D D., "Immunofluorescent analysis of expression of the rna tumor virus major glycoprotein, gp71, on surfaces of virus- -producing murine and other mammalian species cell lines." (1977). Subject Strain Bibliography 1977. 1745 ...
Define indirect fluorescent antibody. indirect fluorescent antibody synonyms, indirect fluorescent antibody pronunciation, indirect fluorescent antibody translation, English dictionary definition of indirect fluorescent antibody. adj. 1. Diverging from a direct course; roundabout. 2. a. Not proceeding straight to the point or object. b. Not forthright and candid; devious. 3.
A glycoprotein with an apparent 340,000 mol wt (gp 340K) was isolated from rat kidney saline-soluble extract by ammonium sulfate precipitation, DE 52 ion-exchange cellulose chromatography, concanavalin A affinity column, Sephacryl S-300 gel filtration, and discontinuous polyacrylamide gel electrophoresis (PAGE). The relative purity of gp 340K was examined by double immunodiffusion analysis, disc PAGE, and immunoelectrophoresis. Injection of rabbit gp 340K antiserum into pregnant rats during the organogenetic period induced abnormal embryonic development, fetal growth retardation, and embryonic death. Antiserum against the immunocomplexes isolated by immobilized protein A also produced the same embryotoxic effects. The biologic effects of the antisera appeared to be dose dependent. Defects such as anophthalmia, hydrocephaly, exencephaly, cleft palate, cleft lip, and some cardiovascular anomalies were observed. The most frequently observed anomaly was anophthalmia. Immunofluorescent localization ...
Dynein tethers centrosomes to spindle poles. (a) Cytoplasmic dynein is eluted from spindles by addition of mAb 70.1. Immunofluorescent localization of dynei
Learning Objectives Describe the benefits of immunofluorescent antibody assays in comparison to nonfluorescent assays Compare direct and indirect flu
Immunofluorescence localization and immunoblotting of Gαs in oocytes in Gpr3+/+ and Gpr3−/− ovaries. (A and B) Gαs in Gpr3+/+ (A) and Gpr3−/− (B) oocy
A monoclonal antibody, designated C138, was raised against a particulate fraction from young postnatal mouse cerebellum. In sections from adult mouse brain and cerebellum, this antibody stained a sub-population of neurons. Cell bodies of large neurones and fibre tracts were negative in all brain regions examined, whereas neuropil was heavily labelled. Immune-electron microscopy confirmed the specificity of the antibody for certain types of neurones and indicated that the antigen may be associated with microtubular structures. Immunofluorescence studies on dissociated cerebellar cultures showed that the antigen was expressed inside all tetanus toxin-positive neurones but not by non-neuronal cells.
Facilities for diagnosis of rabies through RT-PCR, mice-inoculation, cultural isolation and, fluorescent antibody technique have been created and is being used on a regular basis. ...
annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...
annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...
annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...
Fluorescent controls are useful for visual indication of delivery, and when combined with functional knockdown assessment, provide a useful tool for optimization.. siGLO Positive controls will effectively silence the indicated gene, and result in punctate cytoplasmic fluorescence. All are labeled with DY-547 (Cy3 analog). siGLO RISC-Free Control is a DY-547-labeled negative control that can also be co-transfected with functional siRNAs.. siGLO Transfection Indicators are unique non-RISC engaging molecules that localize to the nucleus, providing a distinct visual indication of transfection success. They are available with either DY-547 (Red) or 6-FAM (Green).. ...
... is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope. - Immunofluorescence - AbVideo™ - Support - Abnova
The cellular localization of gastrin in the rabbit pyloric antrum was established by immunofluorescence. The gastrin cell was argyrophil (Grimelius technique) and identical with a previously described cell type that emits fluorescence upon combined formaldehydeozone treatment, a feature that has been interpreted as indicating storage of peptides with NH2-terminal tryptophan. The identity of the peptides and its relation to gastrin is unknown.
Today is exciting because I get to do two things. 1: Post the first successful images of West Nile virus infectined cells that I took on my own and 2: Give a basic explanation one of the most visually impressive techniques at our disposal: immunofluorescent microscopy. For those of you not familiar with fluorescent microscopy…
BD Difco™ Fluorescent Antibody Reagents FA Bordetella Pertussis; 5mL BD Difco™ Fluorescent Antibody Reagents Antibody Binding Proteins and...
This is an historical archive of the activities of the MRC Anatomical Neuropharmacology Unit (MRC ANU) that operated at the University of Oxford from 1985 until March 2015. The MRC ANU established a reputation for world-leading research on the brain, for training new generations of scientists, and for engaging the general public in neuroscience. The successes of the MRC ANU are now built upon at the MRC Brain Network Dynamics Unit at the University of Oxford.. ...
As with most fluorescence techniques, a significant problem with immunofluorescence is photobleaching. Loss of activity caused by photobleaching can be controlled by reducing or limiting the intensity or time-span of light exposure, by increasing the concentration of fluorophores, or by employing more robust fluorophores that are less prone to bleaching (e.g., Alexa Fluors, Seta Fluors, or DyLight Fluors). Some problems that may arise from this technique include autofluorescence, extraneous undesired specific fluorescence, and nonspecific fluorescence. Autofluorescence includes fluorescence emitted from the sample tissue or cell itself. Extraneous undesired specific fluorescence occurs when a targeted antigen is impure and contains antigenic contaminants. Nonspecific fluorescence involves the loss of a probes specificity due to fluorophore, from improper fixation, or from a dried out specimen.[3]. Immunofluorescence is only limited to fixed (i.e., dead) cells when structures within the cell are ...
The results of antinuclear antibody tests using the indirect immunofluorescence technique may be reported as a description of the pattern and the intensity of fluorescence obtained at a certain dilution. If quantitative results are required titration is necessary. Such titrations may vary greatly between different laboratories. The present study involving 26 laboratories shows an improvement of interlaboratory comparability for the homogeneous fluorescence pattern when a common reference serum is used. Cultured cells as substrate appear to give better quantitative agreement than rat liver sections. National reference sera should be standardised in items of the appropriate WHO reference preparation. ...
Immunofluorescence procedures for SNs and rat cortical neurons followed those of Chin et al. (1999). Briefly, cells were fixed in a solution of 4% paraformaldehyde in PBS containing 20% sucrose. After three rinses in PBS, fixed cells were blocked for 30 min at room temperature in Superblock buffer (Pierce), 0.2% Triton X-100, and 3% normal goat serum and subsequently incubated overnight at 4°C with anti-phosphorylated ERK (pERK) antibody (1:500), anti-phosphorylated p38 (p-p38) MAPK antibody (1:200), anti-MAPK phosphatase 1 (MKP1) antibody (1:500), or anti-pCREB2 antibody (1:500). Anti-pERK antibody was purchased from Cell Signaling Technology. Anti-p-p38 MAPK and anti-MKP1 antibodies were purchased from Santa Cruz Biotechnology. The anti-pCREB2 antibody was raised by a commercial vendor (Genemed Biotechnologies) against the phosphorylated version of a CREB2 hybrid peptide (SPPDSPEQGPSSPET) constructed to juxtapose the sequences immediately surrounding two putative MAPK phosphorylation sites ...
We recently teamed-up with Virology Research Services (VRS) to test a large panel of viral antibodies in immunofluorescence applications. This work was funded by the Medical Research Council (Proximity to Discovery Award for Knowledge Exchange) through the University College London Translational Research Office and aimed to improve utilisation of the available immunofluorescence resources.
KPL DyLight conjugates offer a brilliant choice in a variety of immunofluorescence detection applications. They combine the sensitivity and reproducibility of our affinity purified secondary antibodies with a series of DyLight dyes that span the light spectra from visible to infrared. They are brighter than fluorescein, rhodamine, Cy™3 and Cy5 and offer comparable brightness and photostability to Alexa conjugates. They are ideal for use in the following applications:. • Fluorescent Western ...
Carboxypeptidase O (CPO), a member of the M14 family of proteolytic enzymes, preferentially cleaves C-terminal acidic amino acids, with weak affinity towards hydrophobic amino acids. We investigated the subcellular localization of CPO, and after immunofluorescent analysis of stably-transfected MDCK cells, we found that CPO co-localized with calnexin, an ER marker. To determine what CPO does in the ER, MDCK cells were transfected with plasmids expressing Gaussia Luciferase (GLuc) containing a C-terminal ER retention signal (KDEL). Previous experiments suggested that CPO cleaves the KDEL sequence of GLuc, causing its secretion. In ongoing experiments, plasmids expressing GLuc tagged with modified KDEL sequences (KDELD, KDELE, and KDELEL) will be transfected, and the intracellular activity of CPO against these substrates will be assessed.
It is well known that this reagent leads to a depletion of extracellular Ca2+ which in turn causes a disassembly of tight junction [1, 2]. The latter is reflected by a significant drop in the TER readings. Subsequent replacement of the EGTA containing medium by standard medium led to a regeneration of the tight junctions network as revealed by increasing TER readings. For validation of the temporary break down of the barrier function two cell cultures were fixed just before removal of EGTA. Samples were then stained for immunofluorescent analysis of cell nuclei and ZO-1 proteins. Imaging by Confocal Laser Scanning Microscopy and comparison with the untreated reference cell culture clearly revealed the disintegration of the tight junctions network induced by EGTA exposure. These findings are in excellent agreement with the TER results and demonstrate the benefits of using a label-free and noninvasive technique as implemented in the cellZscope.. Cell layer formation. Application of impedance ...
Freshly isolated peripheral blood mononuclear cells (PBMC) from 10 healthy volunteers, 28 patients with rheumatoid arthritis (RA), eight patients with osteoarthritis, and five patients with ankylosing spondylitis were examined for interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) production using monoclonal antibodies and an indirect immunofluorescent method. In freshly isolated PBMC from healthy controls very few cells were stained for either IL-1 type. All 20 RA patients who were not receiving parenteral gold therapy had PBMC staining for IL-1 alpha. In these patients, up to 7.5% of PBMC showed bright IL-1 alpha staining (range 1.2-7.5%). No IL-1 beta staining was seen. These IL-1 alpha-staining cells had a dendritic morphology and the percentage of cells staining correlated well with levels of C-reactive protein, an index of disease activity in these RA patients. Significantly fewer IL-1 alpha-staining cells were present in the peripheral blood of RA patients receiving gold therapy
Spa2p and Cdc10p both participate in bud site selection and cell morphogenesis in yeast, and spa2delta cdc10-10 cells are inviable. To identify additional components important for these processes in yeast, a colony-sectoring assay was used to isolate high-copy suppressors of the spa2delda cdc10-10 lethality. One such gene, AXL2, has been characterized in detail. axl2 cells are defective in bud site selection in haploid cells and bud in a bipolar fashion. Genetic analysis indicates that AXL2 falls into the same epistasis group as BUD3. Axl2p is predicted to be a type I transmembrane protein. Tunicamycin treatment experiments, biochemical fractionation and extraction experiments, and proteinase K protection experiments collectively indicate that Axl2p is an integral membrane glycoprotein at the plasma membrane. Indirect immunofluorescence experiments using either Axl2p tagged with three copies of a hemagglutinin epitope or high-copy AXL2 and anti-Axl2p antibodies reveal a unique localization ...
Immunofluorescence localization was performed as previously described.31 Briefly, corneas were fixed with cold methanol or 4% paraformaldehyde in PBS, cryoprotected with sucrose-PBS in a series of dilutions (10%, 20%, and then 30%), embedded and frozen in OCT medium (Sakura Finetek, Torrance, CA, USA). Cross sections of 6 μm were cut using a cryostat (Microm HM 505E; GMI, Ramsey, MN, USA) followed by immunofluorescence localization. For corneal flat mounts, freshly enucleated corneas were removed under an operating microscope (Carl Zeiss Microscopy, Oberkochen, Germany). Cornea flat mounts were digested in 1 mg/mL collagenase solution (Worthington Biochemical Corporation, Lakewood, NJ, USA) in PBS for 7 minutes at 37°C to facilitate antibody penetration into the tissue. No collagenase was used for BrdU or Ki-67 flat mounts which were fixed in cold methanol. For BrdU staining, tissue was treated with 2N HCl at 37°C for 15 minutes to denature DNA and neutralized in boric acid (pH 8.5) 3 times ...
Methods and Materials This review targets the role of antibody methods and sialylation because of its quantitation. this lectin-affinity small fraction holds the entire anti-inflammatory activity, with the nonbinding fraction being essentially ineffective. At first glance, these results appear to match nicely with the 11% of sialylated N-glycans found in the Fc region [8, 22]. The situation, however, is more complex. Site-specific analysis of the SNA binding and nonbinding fractions of IVIG revealed no significant difference in Fc sialylation [22]. The obvious conclusion was that the fractionation of IgG on SNA was solely based on the N-glycans in the variable domains, whereas the sialoglycans in the CH2 domain name were inaccessible to the lectin. This view was seemingly supported by SNA fractionation of isolated Fab fragments [22]. However, it harshly contradicts the earlier conclusion that this anti-inflammatory activity depended on sialylation of the Fc region N-glycans [3, 4]. Stadlmann and ...
You can use an immunoassay like a homogeneous sandwich FRET assay: You can use donor-dye-labelled fluorophore antibodies that are able to recognise specific antigens on the virus to bind the virus antigen, then introduce a second set of antibodies labelled with acceptor-dye fluorophore. If the vaccine works and produces an immune response in the in-vivo model that destroys the virus, the antigen that joins the donor-labelled and acceptor-labelled antibodies is also destroyed and can no longer "sandwich" the two antibodies close together within the Förster distance to exhibit FRET.. ...
The culture of African green monkey kidney fibroblasts that appears in this section was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Rhodamine Red-X.
annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...
The culture of sheep kidney cells (MDOK line) illustrated in this section was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to fluorescein.
annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...
annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...
By means of the indirect membrane immunofluorescence test, the distribution and antibody-induced redistribution (patching and capping) of a mammary tumor virus-induced (MLr) and a normal (Thy 1.2) cell-surface antigen were compared on mouse thymocytes and leukemia cells (GRSL2). At 0 degrees C Thy 1.2 fluorescence was ringlike and more intense on GRSL2 cells than on thymocytes, whereas MLr fluorescence on GRLS2 cells at this temperature was patchlike and brighter than Thy 1.2 fluorescence. At 20 or 37 degrees C, capping of Thy 1.2 on both cell types was readily achieved but MLr capping occurred only in a few GRSL2 cells and was less pronounced. However, after addition of the secondary antibodies, MLr capping was markedly increased by gradual cooling of cells to about 17 degrees C. Conversely, after addition of antibodies at 0 degrees C, gradual warming of cells under the fluorescence microscope resulted in extensive capping both of MLr and Thy 1.2 at approximately 13-14 degrees C. Rapid cooling ...
Research Proven Rabbit Polyclonal phospho-Tyrosine Hydoxylase-TH antibody. This an excellent neural stem cell and neural marker. This TH is desigend for immunohistochemsitry, immunofluoresence and Western Blotting.
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Publikations-Datenbank der Fraunhofer Wissenschaftler und Institute: Aufsätze, Studien, Forschungsberichte, Konferenzbeiträge, Tagungsbände, Patente und Gebrauchsmuster
IFA kits allow you to test for autoimmune, bacterial, and viral diseases, as well as parasites. Find quality kits when you shop online at RapidTest.
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If a certain cell type is expressing a particular receptor, there are usually many examples of a given receptor type on a cell surface. It is possible that there will be a single receptor on the cell surface, but this is an unlikely case; it would be expected to occur just at the onset of expression as the first receptor arrives at the cell surface or would be expected to occur after expression has halted, as the receptors are being degraded by proteases during normal protein turnover and finally only one is left. Receptor concentrations can be roughly estimated by immunohistochemistry (e.g. fluorescent antibodies) but are more rigorously quantitated by Western blot (which requires a larger sample, more cells ...
If a certain cell type is expressing a particular receptor, there are usually many examples of a given receptor type on a cell surface. It is possible that there will be a single receptor on the cell surface, but this is an unlikely case; it would be expected to occur just at the onset of expression as the first receptor arrives at the cell surface or would be expected to occur after expression has halted, as the receptors are being degraded by proteases during normal protein turnover and finally only one is left. Receptor concentrations can be roughly estimated by immunohistochemistry (e.g. fluorescent antibodies) but are more rigorously quantitated by Western blot (which requires a larger sample, more cells ...
... is a very sensitive and versatile method used to label specific molecular targets within cells and tissues through combined use of specific antibodies and chemical fluorescent tags
Multiplex immunofluorescence (mIF) combines the spatial information from immunohistochemistry (IHC) with multimarker phenotypes. Recent advances in mIF technology have made it possible for researchers to develop novel custom panels, but additional considerations must be taken into account in order to produce a panel which performs at least as well as IHC on a marker-by-marker basis and avoids any undesirable interactions between detection of the targets and neighboring visible light spectra.<
A quantitative, indirect, fluorescence immunoassay (FIAX; Whittaker Bioproducts, Inc.) was compared with the conventional indirect fluorescent-antibody test for detection of serum antibody to Borrelia burgdorferi. FIAX correlated well with the indirect fluorescent-antibody test (r = 0.72). FIAX is a convenient and dependable means of measuring serum antibody to B. burgdorferi. ...
A quantitative, indirect, fluorescence immunoassay (FIAX; Whittaker Bioproducts, Inc.) was compared with the conventional indirect fluorescent-antibody test for detection of serum antibody to Borrelia burgdorferi. FIAX correlated well with the indirect fluorescent-antibody test (r = 0.72). FIAX is a convenient and dependable means of measuring serum antibody to B. burgdorferi.
TY - JOUR. T1 - Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method. AU - Noda, N.. AU - Ikuta, H.. AU - Ebie, Y.. AU - Hirata, A.. AU - Tsuneda, Satoshi. AU - Matsumura, M.. AU - Sumino, T.. AU - Inamori, Y.. PY - 2000. Y1 - 2000. N2 - Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed bacterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO 14298) and sixteen against Nitrobacter winogradskyi (IFO 14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and ...
Figure 3. Immunofluorescent localization of procollagen I and Hsp47 in the presence or absence of brefeldin A treatment in CEC. Cells were treated with 2 µg/ml brefeldin A for 30 min, fixed, permeabilized, and stained as described in the text. A. Procollagen I (green) and Hsp47 (red) in brefeldin A-treated cells. B. Prolyl 4-hydroxylase (green) and procollagen I (red) in brefeldin A-treated cells. C. Prolyl 4-hydroxylase (green) and Hsp47 (red) in brefeldin A-treated cells. D. Phase-contrast microscopy of C. Bar, 10 µm.. ...
A procedure has been developed for the determination of the concentration of infective Newcastle disease virus (NDV) based on the enumeration of singly infected and distributed HeLa cells which are visualized by staining with fluorescent antibody. Infective virus assayed by the fluorescent cell-counting procedure is expressed in terms of cell-infecting units (CIU).. Adsorption of NDV to HeLa cell monolayers reached a plateau 1 to 1.5 hours after inoculation of coverslip cultures, and 12 per cent of the infective particles inoculated failed to adsorb. The half-life of NDV in protein-free Eagles medium at 37°C. was 2.1 hours. There was a linear relationship between virus concentration and the number of infected cells. The coefficient of variation of the mean of replicate determinations of infective NDV was 8.2 per cent. The distribution of single infected HeLa cells in the monolayer corresponded to the Poisson distribution. With NDV the cell-infecting unit (CIU) determined in HeLa cells is ...
The SHV-1 β-lactamase gene was sub-cloned into the pBC SK(-) vector (Stratagene, LaJolla, CA) from a clinical strain of K. pneumoniae (15571), and transformed into E. coli DH10B cells (Invitrogen, Carlsbad, CA) [15]. The K. pneumoniae clinical isolate possessed the SHV-5 ESBL and was obtained from a previous study [16]. E. coli DH10B without the blaSHV-1 gene served as a negative control.. The procedures used to isolate, express and purify the SHV-1 β-lactamase and to produce the anti-SHV β-lactamase antibodies have been previously detailed [13]. Purified anti-SHV antibodies were fluorescein-labeled with the EZ-Label™ fluorescent labeling kit (Pierce, Rockford, IL), according to the instructions of the manufacturer. In brief, 1 mg of polyclonal anti-SHV antibodies in 1 ml phosphate buffered saline (PBS, 2 mM monobasic sodium phosphate, 8 mM dibasic sodium phosphate, 154 mM sodium chloride, pH 7.4) was mixed with 7.6 μl of a 10 mg/ml solution of NHS-fluorescein in N, N-dimethylformamide for ...
Single-cell analysis reveals aspects of cellular physiology not evident from population-based studies, particularly in the case of highly multiplexed methods such as mass cytometry (CyTOF) able to correlate the levels of multiple signaling, differentiation and cell fate markers. Immunofluorescence (IF) microscopy adds information on cell morphology and the microenvironment that are not obtained using flow-based techniques, but the multiplicity of conventional IF is limited. This has motivated development of imaging methods that require specialized instrumentation, exotic reagents or proprietary protocols that are difficult to reproduce in most laboratories. Here we report a public-domain method for achieving high multiplicity single-cell IF using cyclic immunofluorescence (CycIF), a simple and versatile procedure in which four-color staining alternates with chemical inactivation of fluorophores to progressively build a multichannel image. Because CycIF uses standard reagents and instrumentation ...
Summary Fluorescent antibody technics were used to determine the localization and distribution of Schistosoma mansoni antigen in tissue cells, the presence of circulating antibody, and the sites of antibody production or in vivo antigen-antibody combination. The experiments were performed in mice after a primary infection with cercariae, after several challenges, and after antigen stimulation and challenge. Evidence of the presence of circulating antibody was first observed at 20 days in the inhibition fluorescent antibody test, at 25 days in the indirect fluorescent antibody test, at 42 days with the cercarial fluorescent antibody test, and at 47 days in the Cercarienhullen reaction. Sites of antibody production or in vivo antigen-antibody combination were observed in inflammatory cells of the portal tracts, especially those along the smaller arteries, and in granulomas and isolated cells in the parenchyma of the liver; in the perivascular tissue cells, isolated parenchymal cells and granulomas of the
Synonyms for direct fluorescein-conjugated antibody in Free Thesaurus. Antonyms for direct fluorescein-conjugated antibody. 21 words related to antibody: active site, protein, autoantibody, precipitin, ABO antibodies, Rh antibody, antitoxin, agglutinin, Forssman antibody.... What are synonyms for direct fluorescein-conjugated antibody?
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...
BioAssay record AID 332145 submitted by ChEMBL: Induction of cellular microtubule disrupting activity in rat A10 cells at 1 ug/ml after 24 hrs by indirect immunofluorescence technique.
Efficient cleavage and probe versatility for highly-multiplex detection and co-localization. Modified Lightning Terminators™ provide a robust method for detecting multiple antigens from a single sample. Immunofluorescent detection of antigens in fixed tissue specimens and on immunoblots is used routinely in clinical practice and research laboratories, but current methods are limited to the detection of one to four antigens per tissue section or blot. Consequently, the detection of additional antigens requires multiple independent stains on separate sections and limits the co-localization of antigens on a single section. Whether in immunohistochemistry or immunoblotting, our photocleavable fluorescent labels (PCLs) provide advantages over current methods.. Highly Serial and Multiplex Detection. PCL technology uses our expertise in photocleavable chemistry to provide structures that can both efficiently bind to target antigens and rapidly cleave the dye reporter after exposure to UV light. The ...
Animals, Antilymphocyte Serum, Cell Separation, Complement System Proteins, Fluorescent Antibody Technique, Genetic Linkage, Isoantigens, Killer Cells, Natural, Male, Mice, Mice, Inbred A, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Inbred DBA, Mice, Inbred NZB. ...
Recent investigations have shown the feasibility of applying the fluorescent antibody method to Lancefield grouping of streptococci.1-4 The present study was un
Buy Goat Anti-Rabbit IgG (H+L chain specific) secondary antibody (MBS674759) product datasheet at MyBioSource, Secondary Antibodies. Application: ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry
... is for indirect sensitive immunofluorescent detection of proteins in ICC or IHC via StreptAvidin-Biotin Complex (SABC) method.
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Main Page , Multiple Labeling. It is often useful to be able to stain for two or more antigens in one common tissue section. This can be achieved by immunofluorescence method using different fluorescent dyes. Multiple staining can also be done with peroxidase conjugated antibodies developed with different chromogen substrates to produce the end products of different colors. There are three basic approaches in planning multiple staining: parallel, sequential and adjacent. In addition, the antibody dilution and condition are also important factors to be considered. Finally, appropriate color combination is also crucial since improper color combination may produce poor result and fail to demonstrate multiple antigens in the same section. For best result, the careful design and test of multiple staining protocols are necessary.. ...
The Gibbs laboratory is focused on the development of novel fluorophores and fluorescence imaging technologies to improve cancer detection and treatment. The main research focuses of the group are on fluorophore development for image-guided surgery, fluorescent labeling of small molecule therapeutics to improve understanding of effective cancer therapy and development of highly multiplexed immunofluorescence imaging technologies to permit deep multiplexed imaging on tissues. Current projects in the Gibbs laboratory include (1) development of near infrared nerve-specific fluorophore to improve nerve sparing during radical prostatectomy, (2) contrast agent and imaging methodology development to improve breast cancer margin assessment in the operating room, (3) design and synthesis of fluorescently labeled small molecule therapeutics for personalized therapy prediction in cancer, and (4) development of improved cyclic immunofluorescence methods to permit up to 50 color staining on a single tissue ...
Lab Tests Online - UK is a website written by practising laboratory doctors and scientists to help you understand the many clinical laboratory tests that are used in diagnosis, monitoring and treatment of disease ...
The past couple months, the media has shined light on how dangerous COVID-19 can be, but it seems they have failed to mention the latest developments and simply preyed on the fear of Americans.. Looking back to March 3, WHO said the coronavirus death rate was 3.4%, around 34 times more lethal than that of the seasonal flu. With that given estimate, it was reasonable to close down stores, restaurants and take precautions necessary.. But as more data comes to light with the novel virus, it could be that the projected 3.4% death rate is greatly exaggerated.. A study conducted in early April at Santa Clara county concluded that a fatality rate for the county was around 0.1-0.2%, extremely similar to the seasonal flu. The fatality rate was far lower because of unconfirmed and asymptomatic cases.. However, it is important to not judge a virus fatality rate on just one study. That leads me to an antibody study conducted in New York.. "New York is reporting 15,500 COVID-19 deaths, and if 2.7 million ...
... , with the specific secondary antibody and all the reagents needed for performing IHC-P;IHC-F;ICC
iPSc-EGFRvIII. CLTH/iPSc-EGFRvIII cell line is induced pluripotent stem cell line that stably expresses high level of EGF mutant receptor vIII and puromycin-resistant gen introduced by virus transduction. EGFRvIII is under EF1 promoter control, allowing high levels of protein expression. Immunofluorescent studies indicate expression of transcription factors (Sox2, Oct4, Nanog) as well as surface proteins (Tra1-81 and Tra1-60) characteristic for iPS cells.. ...
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Definition: labeling of antibodies or antigens with fluorescent dyes in cells or tissue sections which are visualized using a fluorescence or confocal microscope.. General Methods & Techniques. Multiple Labeling Methods & Techniques. Antigen-Antibody Specific Applications. Tissue-Cell Specific Applications. Virological Applications. Tumor, Disease & Diagnostic Applications. Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells - By Louise Cramer and Arshad Desai. We typically work with tissue culture, primary mammalian cells, and cell extracts, but the protocols can be adapted to other systems, such as whole embryos or lower eukaryotes.. Double Immunofluorescence Staining (IHC World) - By Giorgio Gattoretti. Any type of tissue is suitable for this technique, as long as the antigenicity for your antigen(s) is preserved. Immunofluorescence Double Staining Method - Parallel Approach (IHC World) - This protocol includes preparation of slides, pretreatments, procedure, ...
Abberior STAR 580 was developed for STED and confocal microscopy in the orange spectral region Abberior STAR 580 is highly photostable and bright orange fl
Help! Sections come off slide during mounting - posted in Histology and Pathology: I am doing immunofluorescence on frozen mouse spleen tissue. Everytime I go to mount the coverslip onto my slide with Vectorshield+DAPI, the tissue moves and seems to disappear. When I look under the microscope, the tissue seems to be smeared, folded and can only see very few nuclei. Protocol: The tissue is cryopreserved using concentrations of sucrose from 5%-20%. The tissue is then embedded in OCT in...
OBJECTIVES--An indirect immunofluorescence technique applied to paraffin embedded tissue sections of lesions containing Donovan bodies was evaluated as a serological test for the diagnosis of granuloma inguinale. METHODS--Sera from patients with proven granuloma inguinale, other sexually acquired genital ulcerations and blood donors from areas where granuloma inguinale is rarely encountered as well as from disease-endemic regions were tested. Sera were tested either unabsorbed or following absorption with whole Klebsiella pneumoniae bacteria. RESULTS--Using unabsorbed sera at a dilution of 1:160 the test was found to have a sensitivity of 100%, specificity of 98%, positive predictive value (PPV) of 89% and negative predictive value (NPV) of 100%. There proved to be no advantage in preabsorbing sera with K. pneumoniae antigen. CONCLUSIONS--In the absence of culture methods for Calymmatobacterium granulomatis, an indirect immunofluorescence technique may prove valuable for the diagnosis of ...
Various methods have been employed in the epidemiological assessment of malaria. In recent years, new serological techniques have suppemented the measurement of spleen rate and parasite rate. Since clasical malariometric indices such as parasite rate and annual parasite incidence were insufficient and not adequately sensitive to assess the progress of control measures, serological methids have been employed. The indirect fluorescent antibody test (IFA) was the serological test employed in this laboratory. Thick blood films of simian malaria parasites, plasmidium cynomolgi bastianellii and p.fieldi, obtained from infected rhesuns monkeys (Macaca mulatta) were used as antigens. The IFA test was shown to be useful to study the antibody levlels of blood donors from different areas in Sri Lanka, to observe the production and persistence of malarial antibodies in man and rhesus monkeys and for epidemiological assessment of malaria in Sri Lanka. The study conducte on 1050 blood donors revealed that ...
Toxoplasmosis in Peru. Geographic prevalence of Toxoplasma gondii antibodies in Peru studied by indirect fluorescent antibody technique.:
WANDERTEY, Dalva Marli Valério et al. Transfusional chagasic infection detected during the execution of a program for the control of Chagas disease in the State of S. Paulo (Brazil). Rev. Saúde Pública [online]. 1992, vol.26, n.3, pp.203-205. ISSN 1518-8787. http://dx.doi.org/10.1590/S0034-89101992000300012.. A system of surveillance for Chagas disease aiming at a systematic investigation of the occurrence of triatominae in human dwellings in S. Paulo, Brazil was proposed. It included a serological survey of residents in house considered to be potencial breeding places for blood-sucking triatomines. Serologically positive cases were observed to be distributed in age groups from 19 years of age upwards. Case-investigation revealed that the infection had been acquired either in S. Paulo in the past or recently in other States. A serologically positive (titre - 128 - IgG) case of an 8-year-old male child, was detected by the Indirect Fluorescent Antibody Technique (IFAT). In S. Paulo State ...
This program is concerned with the quality of indirect immunofluorescence testing of sera for detection of circulating IgG anti-basement membrane zone and anti-intercellular (cell surface) antibodies. The participating physician or laboratory director receives five serum samples and must process these to determine the presence and titer of circulating antibodies using an indirect immunofluorescence technique with monkey esophagus as a substrate and other substrates if desired. The participant records the results including pattern and the titer. Each participant returns the recorded results to Beutner Labs, Inc. The results of all laboratories in the same testing event are number coded, compiled and summarized for quality review. Beutner Labs, Inc. provides this service in cooperation with the Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo. Following completion of specimen tests from Beutner Labs, Inc. participants ...
In contrast to the Consensus Statement for vasculitis-associated ANCA (23), there are no clear guidelines for immunofluorescence detection and interpretation of ANCA patterns in IBD (24). Differentiating between atypical and typical P-ANCA on ethanol-fixed substrates by IIF is still challenging. Previous data regarding formalin-fixed neutrophils in the detection of atypical P-ANCA are scattered and controversial (4, 7, 31). Differences in formaldehyde fixation techniques and the resolution of the immunofluorescence microscope used might be responsible for these contradictions. However, the use of a formalin-fixed substrate was reported as a simple, useful technique for differentiating between ANA and typical P-ANCA, which may occur together (9). Simultaneous reactivity of atypical P-ANCA on formalin- and ethanol-fixed neutrophils and the feasibility of the new microscopic criteria suggested by Terjung et al. (28) have not been systematically studied in IBD (7, 31). ANCA systems that replace ...
Early immunofluorescence studies on the distribution of γ-tubulin noted that more of this protein is associated with mitotic than interphase centrosomes (e.g., Zheng et al. 1991). This difference in γ-tubulin content correlates with the fact that mitotic centrosomes generate about five to ten times more Mts than interphase centrosomes (Snyder and McIntosh 1975; Kuriyama and Borisy 1981). When does the centrosome acquire its additional γ-tubulin so that it can generate enhanced numbers of Mts during mitosis? One possibility is that γ-tubulin gradually accumulates in the centrosome during the cell cycle, but it is maintained in an inactive form until spindle formation. The other possibility is that it is suddenly recruited to the centrosome near the onset of mitosis. The former hypothesis has recently been supported by Dictenberg et al. 1998 who concluded, from an immunofluorescence analysis of fixed synchronized CHO cells, that the amount of pericentrin and γ-tubulin associated with the ...
Chinese hamster ovary cells were seeded in the absence or presence of the polyamine synthesis inhibitor 2-difluoromethylornithine (DFMO). At 1-4 days after seeding, the cells were labelled for 15-120 min with the thymidine analogue bromodeoxyuridine (BrdUrd) and they were then fixed directly after the labelling period. In addition, cells were labelled for 30 min and they were then allowed to progress in BrdUrd-free medium during a defined post-labelling time before fixation. An indirect immunofluorescence technique, using the monoclonal BrdUrd antibody and the intercalating stochiometric DNA stain, propidium iodide, was applied to enable quantification of cellular BrdUrd and DNA contents, respectively, by flow cytometry (FCM). By comparing the mean DNA content of BrdUrd-labelled cells to the mean DNA contents of G(1) and G(2) cells, a relative measure of the position of the BrdUrd-labelled cells was obtained (relative movement). Relative movement data, obtained from control and DFMO-treated ...
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Human Lens Epithelial Cells (HLEpiC) from Creative Bioarray are isolated from the human lens. HLEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HLEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. HLEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLEpiC are guaranteed to further culture in the conditions provided by Creative Bioarray ...
Specific staining protocols10,11 were as follows: Our DeOlmos cupric silver method and nonfluorescent staining methods for AC3 have been described previously.4,8-12 For MBP (1:100; MAB 395; EMD Millipore, Billerica, MA), fractin (1:400; AB3150; EMD Millipore), Iba1 (1:500; 019-18741; Wako Chemicals, Richmond, VA), and CC-1 (1:200; OP80; Calbiochem, San Diego, CA) immunostaining methods, we used the Vectastain Elite ABC kit with Vector VIP as chromogen (Vector Laboratories, Inc., Burlingame, CA).4,8-12 Immunofluorescent detection of caspase-mediated cell death employed an AC3 rabbit primary polyclonal antibody (9661B; 1:500; Cell Signaling Technology, Inc., Danvers, MA). Floating sections were incubated overnight at room temperature. After the sections were rinsed in phosphate-buffered saline (3 × 5 min) they were incubated for 2 h at room temperature with fluorescent goat antirabbit Alexa Fluor 555 (1:1,000, Invitrogen™, Life Technologies™, Grand Island, NY), rinsed in phosphate-buffered ...
A T cell clone (ACH-2) derived from T cells infected with HIV-1 was found to produce HIV-1 in response to stimulation with a monokine-enriched supernatant prepared by culturing human monocyte/macrophages with bacterial LPS (LPS-MO SN). Monokine induction of ACH-2 cells resulted in augmented virus production reflected by an increase in reverse transcriptase activity and in the synthesis of all major viral proteins. Examination of the cells by indirect immunofluorescence revealed that 10 to 15% of uninduced cells constitutively expressed HIV proteins, whereas 100% showed positive immunofluorescence in response to LPS-MO SN. This induction of virus by LPS-MO SN resulted in approximately a 100-fold increase of infectious virus production over uninduced ACH-2 cells. LPS alone could not induce HIV-1 expression, whereas LPS-MO SN resulted in the greatest virus expression. Cell separation studies confirmed the source of the inducing factor(s) to be cells bearing the mature monocyte/macrophage marker, ...
Oxytocin (OT) and vasopressin (AVP) play a major role in social behaviours. Mice have become the species of choice for neurobiology of social behaviour due to identification of mouse pheromones and the advantage of genetically modified mice. However, neuroanatomical data on nonapeptidergic systems in mice are fragmentary, especially concerning the central distribution of OT. Therefore, we analyse the immunoreactivity for OT and its neurophysin in the brain of male and female mice (strain CD1). Further, we combine immunofluorescent detection of OT and AVP to locate cells co-expressing both peptides and their putative axonal processes. The results indicate that OT is present in cells of the neurosecretory paraventricular (Pa) and supraoptic hypothalamic nuclei (SON). From the anterior SON, OTergic cells extend into the medial amygdala, where a sparse cell population occupies its ventral anterior and posterior divisions. Co-expression of OT and AVP in these nuclei is rare. Moreover, a remarkable ...
Hi Eleanor, I can offer one general hint: tetraploid yeast cells are larger than diploids, and it may be possible to make cells of even higher ploidy. I did this long ago (~ 15 years!) to facilitate immunofluorescent detection of a low-abundance protein. Unfortunately I cant remember the details or references at this point (my own experiment didnt work so I never published it), but I think it was straightforward to make the polyploid cells - you just need a lot of markers to select them. Good luck! Maria Maria C. Costanzo, Ph.D. Senior Scientific Curator, Saccharomyces Genome Database Department of Genetics Stanford University School of Medicine Stanford, CA 94305-5120 Phone: 650-725-8956 Fax: 650-723-7016 http://www.yeastgenome.org/ maria at genome.stanford.edu On Thursday, November 13, 2003, at 08:14 PM, wozei wrote: , Hi all, , , does any one have an idea how I could make my yeast [S. cerevisiae] , grow to at , least 5-10 micron size or greater for an experiment I need to carry , out with , ...
A number of previous studies have implied that three herpes simplex virus-encoded nuclear transactivator proteins, IE175 (ICP4), IE110 (ICP0), and IE63 (ICP27), may cooperate in transcriptional and posttranscriptional stimulation of viral gene expression. Using double-label immunofluorescence assays (IFA) in transient expression assays, we have examined the intracellular localization of these three proteins in DNA-transfected cells. The IE110 protein on its own forms spherical punctate domains within the nucleus, whereas the IE175 and IE63 proteins alone give uniform and speckled diffuse patterns, respectively. In infected cells, the IE110 punctate granules have been shown to correspond to novel preexisting subnuclear structures referred to as ND10 domains or PODs that contain a variety of cellular proteins, including SP100 and the PML proto-oncogene product. Cotransfection experiments with wild-type nuclear forms of both IE175 and IE110 provided direct evidence for partial redistribution of ...
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ULight™ labeled anti-rabbit IgG antibody for LANCE® TR-FRET assays. The anti-rabbit IgG antibody is a purified mouse polyclonal antibody.
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0147] The amount of TGF-beta1 protein present in the blood vessel wall was determined by quantitative immunofluoresence, as previously extensively described (see Mosedale et al. (1996) J. Histochem. Cytochem. 44:1043-50 for a comprehensive discussion of the key factors in designing a quantitative immunofluoresence experiment; note that all the recommendations therein were rigorously applied during the experiments presented here). Briefly, five slides were selected according to the Paigen Strategy from each animal, each with two neighbouring 4 μm sections on the slide, and the sections were enclosed with a water-resistant barrier (using a PAP pen; Agar Scientific, UK) such that the enclosed area was approximately equal on all slides. Non-specific antibody binding was then blocked using 3% IgG-free bovine serum albumin (BSA; Sigma Chemical Company) in phosphate-buffered saline (PBS) pH7.4 for 2 hours at room temperature. The blocking solution was then gently removed, and replaced with 50 μl of a ...
Goat anti-rabbit IgG (H&L), F(ab)2 fragment, ALP (alkaline phosphatase) conjugated, min. cross-reactivity to bovine,human, mouse IgG/serum -
References for Abcams Goat Anti-Rabbit IgG Fc (Alkaline Phosphatase) (ab97197). Please let us know if you have used this product in your publication
Anti-Rabbit IgG (H&L) Pre-Adsorbed Secondary Antibody, Guinea Pig Polyclonal, Unconjugated validated in WB, E, IC (ASR1636), Abgent
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a total of 1,554 dogs from 5 countries on 3 continents were tested for antibodies to neospora caninum using an indirect fluorescent antibody test. in australia, overall, 42/451 (9%, 95% confidence interval [ci] 6-12%) dogs were seropositive (melbourne 11/207 [5%, 95% ci 2-9%]; sydney 18/150 [12%, 95% ci 7-18%]; perth 13/94 [14%, 95% ci 8-22%]). antibodies to n. caninum were also detected in dogs in south america (uruguay [20%, 95% ci 16-24%, n = 414]) and sub-saharan africa (tanzania [22%, 95% c ...
There are many home-made serological assays developed by different laboratories. The most frequently used test formats are the indirect fluorescent antibody test (IFA) using frozen sections of adult worms, and immunoenzyme assays (mostly ELISAs) with crude or recombinant antigens from eggs or adult worms.. Diagnostic strategies ...