Species, Research Grants, Research Topics, Publications, Genomes and Genes, Scientific Experts about indirect fluorescent antibody technique

Cloyd, M W.; Bolognesi, D P.; and Bigner, D D., Immunofluorescent analysis of expression of the rna tumor virus major glycoprotein, gp71, on surfaces of virus- -producing murine and other mammalian species cell lines. (1977). Subject Strain Bibliography 1977. 1745 ...

Define indirect fluorescent antibody. indirect fluorescent antibody synonyms, indirect fluorescent antibody pronunciation, indirect fluorescent antibody translation, English dictionary definition of indirect fluorescent antibody. adj. 1. Diverging from a direct course; roundabout. 2. a. Not proceeding straight to the point or object. b. Not forthright and candid; devious. 3.

Immunofluorescent analysis of Heat Shock Protein 84 using Anti-Heat Shock Protein 84 Polyclonal Antibody (Product# PA3-012) shows staining in U251 Cells. Heat Shock Protein 84 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 84 (Product# PA3-012) at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552, Goat Anti-Rabbit). Images were taken at 60X magnification. ...

A glycoprotein with an apparent 340,000 mol wt (gp 340K) was isolated from rat kidney saline-soluble extract by ammonium sulfate precipitation, DE 52 ion-exchange cellulose chromatography, concanavalin A affinity column, Sephacryl S-300 gel filtration, and discontinuous polyacrylamide gel electrophoresis (PAGE). The relative purity of gp 340K was examined by double immunodiffusion analysis, disc PAGE, and immunoelectrophoresis. Injection of rabbit gp 340K antiserum into pregnant rats during the organogenetic period induced abnormal embryonic development, fetal growth retardation, and embryonic death. Antiserum against the immunocomplexes isolated by immobilized protein A also produced the same embryotoxic effects. The biologic effects of the antisera appeared to be dose dependent. Defects such as anophthalmia, hydrocephaly, exencephaly, cleft palate, cleft lip, and some cardiovascular anomalies were observed. The most frequently observed anomaly was anophthalmia. Immunofluorescent localization ...

Dynein tethers centrosomes to spindle poles. (a) Cytoplasmic dynein is eluted from spindles by addition of mAb 70.1. Immunofluorescent localization of dynei

Learning Objectives Describe the benefits of immunofluorescent antibody assays in comparison to nonfluorescent assays Compare direct and indirect flu

Dive into the research topics of An immunofluorescent study of the distribution of fibronectin and laminin during limb regeneration in the adult newt. Together they form a unique fingerprint. ...

Immunofluorescence localization and immunoblotting of Gαs in oocytes in Gpr3+/+ and Gpr3−/− ovaries. (A and B) Gαs in Gpr3+/+ (A) and Gpr3−/− (B) oocy

A monoclonal antibody, designated C138, was raised against a particulate fraction from young postnatal mouse cerebellum. In sections from adult mouse brain and cerebellum, this antibody stained a sub-population of neurons. Cell bodies of large neurones and fibre tracts were negative in all brain regions examined, whereas neuropil was heavily labelled. Immune-electron microscopy confirmed the specificity of the antibody for certain types of neurones and indicated that the antigen may be associated with microtubular structures. Immunofluorescence studies on dissociated cerebellar cultures showed that the antigen was expressed inside all tetanus toxin-positive neurones but not by non-neuronal cells.

Lab Reagents. Fluorescent Laboratories manufactures the fluorescent antibody reagents distributed by Genprice. The Fluorescent Antibody reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact Fluorescent. Other Fluorescent products are available in stock. Specificity: Fluorescent Category: Antibody. Lab Tools information ...

Facilities for diagnosis of rabies through RT-PCR, mice-inoculation, cultural isolation and, fluorescent antibody technique have been created and is being used on a regular basis. ...

Tojasida Mol Talk combines the advantages of an easy to learn dinamicx programmable procedural scripting with the flexibility and power of a full programming language. Repeatability was also examined to ensure the reliability of the preparation method. It is designed for easy and rapid image manipulation and display of protein molecules, and is intended for users who need to quickly produce high-resolution images of protein molecules but do not have the time or inclination to use a software molecular visualization system.. This report describes the results for a bilateral comparison of ammonia in nitrogen gas mixture. Double-label immunofluorescence observations on spread BHK cells using this monoclonal antibody and a rabbit polyclonal antibody directed against the 54, and 55, mol -wt proteins showed that themol -wt species co-distributed with IF in a fibrous pattern.. Secondly, the authors do not discuss limitations of their approaches in the discussion. Asco operations staff during all modes ...

annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...

annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...

MACS® GMP CD34 Fluorescent Antibodies consist of a mouse monoclonal antibody against the human CD34 antigen conjugated to a fluorochrome. They are developed for flow cytometric identification and flow cytometric sorting of CD34-positive cells. | Principat dAndorra

annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...

To 2 h TR-OS challenge and remained from the media for your period from the analyze. Cells were being imaged and codistribution analyzed utilizing a binary submask. c M5 (MREG knockdown) and M5 cells transfected with MREG, (these cells are specified (R)) 108341-18-0 Purity & Documentation challenged with TR-OS for 1 h were being fastened, stained for LC3 and MREG. Cells were being imaged and codistribution analyzed working with a binary submask Pearsons coefficient 0.sixty four. The impression quantitation info in this particular figure is really an common of forty cells for every industry, with ten L-Cysteine (hydrochloride) Technical Information fields analyzed in two independent experiments. Mistake bars signify EM (p0.005). C2 details plotted is from visuals demonstrated in Fig. 2. Western blot assessment exhibiting MREG concentrations in C2, M5, and MREG rescue experiments (S. Fig. 3)Creator Manuscript Creator ManuscriptMol Neurobiol. Writer manuscript; 341031-54-7 Formula obtainable in ...

MADRID - Results from the final stage of a nationwide antibody study showed some 5.2% of Spains population has been exposed to the cor...

Fluorescent controls are useful for visual indication of delivery, and when combined with functional knockdown assessment, provide a useful tool for optimization.. siGLO Positive controls will effectively silence the indicated gene, and result in punctate cytoplasmic fluorescence. All are labeled with DY-547 (Cy3 analog). siGLO RISC-Free Control is a DY-547-labeled negative control that can also be co-transfected with functional siRNAs.. siGLO Transfection Indicators are unique non-RISC engaging molecules that localize to the nucleus, providing a distinct visual indication of transfection success. They are available with either DY-547 (Red) or 6-FAM (Green).. ...

Sino Biological offers some good and bad immunofluorescence results of some targeted proteins expressed in the cytoplasm of SHSY5Y cells.

Immunofluorescence is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope. - Immunofluorescence - AbVideo™ - Support - Abnova

The cellular localization of gastrin in the rabbit pyloric antrum was established by immunofluorescence. The gastrin cell was argyrophil (Grimelius technique) and identical with a previously described cell type that emits fluorescence upon combined formaldehydeozone treatment, a feature that has been interpreted as indicating storage of peptides with NH2-terminal tryptophan. The identity of the peptides and its relation to gastrin is unknown.

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Some laboratories offer direct immunofluorescent tests on fresh specimens, but these tests are labor- and personnel-intensive and are less sensitive than culture methods. In order to overcome the expe... more

Kajstura J, Bereiter-Hahn J. Scanning microfluorometric measurement of immunofluorescently labelled microtubules in cultured cells. Dependence of microtubule content on cell density. Histochemistry. 1987; 88(1):53-5 ...

Today is exciting because I get to do two things. 1: Post the first successful images of West Nile virus infectined cells that I took on my own and 2: Give a basic explanation one of the most visually impressive techniques at our disposal: immunofluorescent microscopy. For those of you not familiar with fluorescent microscopy…

TY - JOUR. T1 - Fine structure and immunofluorescent studies of the wish cell line. AU - Meek, William D.. AU - Davis, Walter L.. PY - 1986/12/1. Y1 - 1986/12/1. N2 - Morphological studies of the WISH cell line reveal an epithelioid cell type with some characteristics of both the original human amnion epithelium and a transformed state. WISH cells have a cytoplasm filled with microtubules; however, actin filament bundles are few, with actin localized at areas of cell contact and arranged diffusely through the cytoplasm, as viewed by indirect immunofluorescence. Fingerlike projections or short filopodia are observed connecting cells that grow in a closely apposed monolayer. Other surface features, as viewed by scanning electron microscopy, include microvilli and blebs. Transmission electron microscopy shows that WISH cultures consist of light or dark cells with organelles that include lipid droplets, abundant free ribosomes, tubular mitochondria, lysosomes, annulate lamellae, rough endoplasmic ...

BD Difco™ Fluorescent Antibody Reagents FA Bordetella Pertussis; 5mL BD Difco™ Fluorescent Antibody Reagents Antibody Binding Proteins and...

This is an historical archive of the activities of the MRC Anatomical Neuropharmacology Unit (MRC ANU) that operated at the University of Oxford from 1985 until March 2015. The MRC ANU established a reputation for world-leading research on the brain, for training new generations of scientists, and for engaging the general public in neuroscience. The successes of the MRC ANU are now built upon at the MRC Brain Network Dynamics Unit at the University of Oxford.. ...

As with most fluorescence techniques, a significant problem with immunofluorescence is photobleaching. Loss of activity caused by photobleaching can be controlled by reducing or limiting the intensity or time-span of light exposure, by increasing the concentration of fluorophores, or by employing more robust fluorophores that are less prone to bleaching (e.g., Alexa Fluors, Seta Fluors, or DyLight Fluors). Some problems that may arise from this technique include autofluorescence, extraneous undesired specific fluorescence, and nonspecific fluorescence. Autofluorescence includes fluorescence emitted from the sample tissue or cell itself. Extraneous undesired specific fluorescence occurs when a targeted antigen is impure and contains antigenic contaminants. Nonspecific fluorescence involves the loss of a probes specificity due to fluorophore, from improper fixation, or from a dried out specimen.[3]. Immunofluorescence is only limited to fixed (i.e., dead) cells when structures within the cell are ...

The results of antinuclear antibody tests using the indirect immunofluorescence technique may be reported as a description of the pattern and the intensity of fluorescence obtained at a certain dilution. If quantitative results are required titration is necessary. Such titrations may vary greatly between different laboratories. The present study involving 26 laboratories shows an improvement of interlaboratory comparability for the homogeneous fluorescence pattern when a common reference serum is used. Cultured cells as substrate appear to give better quantitative agreement than rat liver sections. National reference sera should be standardised in items of the appropriate WHO reference preparation. ...

Immunofluorescence procedures for SNs and rat cortical neurons followed those of Chin et al. (1999). Briefly, cells were fixed in a solution of 4% paraformaldehyde in PBS containing 20% sucrose. After three rinses in PBS, fixed cells were blocked for 30 min at room temperature in Superblock buffer (Pierce), 0.2% Triton X-100, and 3% normal goat serum and subsequently incubated overnight at 4°C with anti-phosphorylated ERK (pERK) antibody (1:500), anti-phosphorylated p38 (p-p38) MAPK antibody (1:200), anti-MAPK phosphatase 1 (MKP1) antibody (1:500), or anti-pCREB2 antibody (1:500). Anti-pERK antibody was purchased from Cell Signaling Technology. Anti-p-p38 MAPK and anti-MKP1 antibodies were purchased from Santa Cruz Biotechnology. The anti-pCREB2 antibody was raised by a commercial vendor (Genemed Biotechnologies) against the phosphorylated version of a CREB2 hybrid peptide (SPPDSPEQGPSSPET) constructed to juxtapose the sequences immediately surrounding two putative MAPK phosphorylation sites ...

We recently teamed-up with Virology Research Services (VRS) to test a large panel of viral antibodies in immunofluorescence applications. This work was funded by the Medical Research Council (Proximity to Discovery Award for Knowledge Exchange) through the University College London Translational Research Office and aimed to improve utilisation of the available immunofluorescence resources.

KPL DyLight conjugates offer a brilliant choice in a variety of immunofluorescence detection applications. They combine the sensitivity and reproducibility of our affinity purified secondary antibodies with a series of DyLight dyes that span the light spectra from visible to infrared. They are brighter than fluorescein, rhodamine, Cy™3 and Cy5 and offer comparable brightness and photostability to Alexa conjugates. They are ideal for use in the following applications:. • Fluorescent Western ...

Fig. 2- Representative images for the dual immunohistochemical and immunofluorescence detection of CD4+FOXP+ T-regulatory cells within human lung tissue. Cell nuclei were counterstained with either a-f) 4,6-diamidino-2-phenylindole (blue stain) or g and h) Mayers haematoxylin (blue stain). a) CD4+ cells were identified using an Alexa-488 conjugated goat anti-mouse secondary antibody (red stain) and FOXP3+ cells were detected using 3,3′-diaminobenzidine following indirect immunohistochemistry (brown stain). b) Increased magnification of the highlighted area in (a) demonstrating the distinct nuclear staining of FOXP3 (brown stain) in cells that are also CD4+ (red stain, indicated by arrow). c) A single CD4+FOXP3+ cell (arrow) detected within an airway submucosa. d) A CD4+FOXP3+ cell detected within a population of CD4+FOXP3- T-cells within an alveolar septum. e) A lymphoid follicle expressing an abundance of CD62L throughout with a few CD62L+FOXP3+ cells (arrow). f) Sub-epithelial FOXP3+ cell ...

Carboxypeptidase O (CPO), a member of the M14 family of proteolytic enzymes, preferentially cleaves C-terminal acidic amino acids, with weak affinity towards hydrophobic amino acids. We investigated the subcellular localization of CPO, and after immunofluorescent analysis of stably-transfected MDCK cells, we found that CPO co-localized with calnexin, an ER marker. To determine what CPO does in the ER, MDCK cells were transfected with plasmids expressing Gaussia Luciferase (GLuc) containing a C-terminal ER retention signal (KDEL). Previous experiments suggested that CPO cleaves the KDEL sequence of GLuc, causing its secretion. In ongoing experiments, plasmids expressing GLuc tagged with modified KDEL sequences (KDELD, KDELE, and KDELEL) will be transfected, and the intracellular activity of CPO against these substrates will be assessed.

It is well known that this reagent leads to a depletion of extracellular Ca2+ which in turn causes a disassembly of tight junction [1, 2]. The latter is reflected by a significant drop in the TER readings. Subsequent replacement of the EGTA containing medium by standard medium led to a regeneration of the tight junctions network as revealed by increasing TER readings. For validation of the temporary break down of the barrier function two cell cultures were fixed just before removal of EGTA. Samples were then stained for immunofluorescent analysis of cell nuclei and ZO-1 proteins. Imaging by Confocal Laser Scanning Microscopy and comparison with the untreated reference cell culture clearly revealed the disintegration of the tight junctions network induced by EGTA exposure. These findings are in excellent agreement with the TER results and demonstrate the benefits of using a label-free and noninvasive technique as implemented in the cellZscope.. Cell layer formation. Application of impedance ...

Freshly isolated peripheral blood mononuclear cells (PBMC) from 10 healthy volunteers, 28 patients with rheumatoid arthritis (RA), eight patients with osteoarthritis, and five patients with ankylosing spondylitis were examined for interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) production using monoclonal antibodies and an indirect immunofluorescent method. In freshly isolated PBMC from healthy controls very few cells were stained for either IL-1 type. All 20 RA patients who were not receiving parenteral gold therapy had PBMC staining for IL-1 alpha. In these patients, up to 7.5% of PBMC showed bright IL-1 alpha staining (range 1.2-7.5%). No IL-1 beta staining was seen. These IL-1 alpha-staining cells had a dendritic morphology and the percentage of cells staining correlated well with levels of C-reactive protein, an index of disease activity in these RA patients. Significantly fewer IL-1 alpha-staining cells were present in the peripheral blood of RA patients receiving gold therapy

Spa2p and Cdc10p both participate in bud site selection and cell morphogenesis in yeast, and spa2delta cdc10-10 cells are inviable. To identify additional components important for these processes in yeast, a colony-sectoring assay was used to isolate high-copy suppressors of the spa2delda cdc10-10 lethality. One such gene, AXL2, has been characterized in detail. axl2 cells are defective in bud site selection in haploid cells and bud in a bipolar fashion. Genetic analysis indicates that AXL2 falls into the same epistasis group as BUD3. Axl2p is predicted to be a type I transmembrane protein. Tunicamycin treatment experiments, biochemical fractionation and extraction experiments, and proteinase K protection experiments collectively indicate that Axl2p is an integral membrane glycoprotein at the plasma membrane. Indirect immunofluorescence experiments using either Axl2p tagged with three copies of a hemagglutinin epitope or high-copy AXL2 and anti-Axl2p antibodies reveal a unique localization ...

Intermediate Filaments;Sodium Dodecyl Sulfate;Antibodies;Retinitis Pigmentosa;Fluorescent Antibody Technique, Indirect;Microscopy, Fluorescence;Electrophoresis, Polyacrylamide Gel;Spinal ...

Immunofluorescence localization was performed as previously described.31 Briefly, corneas were fixed with cold methanol or 4% paraformaldehyde in PBS, cryoprotected with sucrose-PBS in a series of dilutions (10%, 20%, and then 30%), embedded and frozen in OCT medium (Sakura Finetek, Torrance, CA, USA). Cross sections of 6 μm were cut using a cryostat (Microm HM 505E; GMI, Ramsey, MN, USA) followed by immunofluorescence localization. For corneal flat mounts, freshly enucleated corneas were removed under an operating microscope (Carl Zeiss Microscopy, Oberkochen, Germany). Cornea flat mounts were digested in 1 mg/mL collagenase solution (Worthington Biochemical Corporation, Lakewood, NJ, USA) in PBS for 7 minutes at 37°C to facilitate antibody penetration into the tissue. No collagenase was used for BrdU or Ki-67 flat mounts which were fixed in cold methanol. For BrdU staining, tissue was treated with 2N HCl at 37°C for 15 minutes to denature DNA and neutralized in boric acid (pH 8.5) 3 times ...

Methods and Materials This review targets the role of antibody methods and sialylation because of its quantitation. this lectin-affinity small fraction holds the entire anti-inflammatory activity, with the nonbinding fraction being essentially ineffective. At first glance, these results appear to match nicely with the 11% of sialylated N-glycans found in the Fc region [8, 22]. The situation, however, is more complex. Site-specific analysis of the SNA binding and nonbinding fractions of IVIG revealed no significant difference in Fc sialylation [22]. The obvious conclusion was that the fractionation of IgG on SNA was solely based on the N-glycans in the variable domains, whereas the sialoglycans in the CH2 domain name were inaccessible to the lectin. This view was seemingly supported by SNA fractionation of isolated Fab fragments [22]. However, it harshly contradicts the earlier conclusion that this anti-inflammatory activity depended on sialylation of the Fc region N-glycans [3, 4]. Stadlmann and ...

You can use an immunoassay like a homogeneous sandwich FRET assay: You can use donor-dye-labelled fluorophore antibodies that are able to recognise specific antigens on the virus to bind the virus antigen, then introduce a second set of antibodies labelled with acceptor-dye fluorophore. If the vaccine works and produces an immune response in the in-vivo model that destroys the virus, the antigen that joins the donor-labelled and acceptor-labelled antibodies is also destroyed and can no longer sandwich the two antibodies close together within the Förster distance to exhibit FRET.. ...

The culture of African green monkey kidney fibroblasts that appears in this section was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Rhodamine Red-X.

annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...

annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...

The culture of sheep kidney cells (MDOK line) illustrated in this section was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to fluorescein.

A quantitative, indirect, fluorescence immunoassay (FIAX; Whittaker Bioproducts, Inc.) was compared with the conventional indirect fluorescent-antibody test for detection of serum antibody to Borrelia burgdorferi. FIAX correlated well with the indirect fluorescent-antibody test (r = 0.72). FIAX is a convenient and dependable means of measuring serum antibody to B. burgdorferi. ...

A quantitative, indirect, fluorescence immunoassay (FIAX; Whittaker Bioproducts, Inc.) was compared with the conventional indirect fluorescent-antibody test for detection of serum antibody to Borrelia burgdorferi. FIAX correlated well with the indirect fluorescent-antibody test (r = 0.72). FIAX is a convenient and dependable means of measuring serum antibody to B. burgdorferi.

TY - JOUR. T1 - Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method. AU - Noda, N.. AU - Ikuta, H.. AU - Ebie, Y.. AU - Hirata, A.. AU - Tsuneda, Satoshi. AU - Matsumura, M.. AU - Sumino, T.. AU - Inamori, Y.. PY - 2000. Y1 - 2000. N2 - Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed bacterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO 14298) and sixteen against Nitrobacter winogradskyi (IFO 14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and ...

Figure 3. Immunofluorescent localization of procollagen I and Hsp47 in the presence or absence of brefeldin A treatment in CEC. Cells were treated with 2 µg/ml brefeldin A for 30 min, fixed, permeabilized, and stained as described in the text. A. Procollagen I (green) and Hsp47 (red) in brefeldin A-treated cells. B. Prolyl 4-hydroxylase (green) and procollagen I (red) in brefeldin A-treated cells. C. Prolyl 4-hydroxylase (green) and Hsp47 (red) in brefeldin A-treated cells. D. Phase-contrast microscopy of C. Bar, 10 µm.. ...

Parafusin (PFUS), a superfamily member of phosphoglucomutase (PGM), is involved in the exocytic nano-machinery. It is a 63kD phosphoglycoprotein with two cyclic covalent modifications (phosphorylation/dephosphorylation and phosphoglucosylation/dephosphoglucosylation). It is a component of the scaffold associated with dense core secretory vesicles (DCSV). This thesis focuses on the role of PFUS and its post-translational modifications in exocytosis.;To conclusively establish the importance of PFUS in exocytosis, PFUS RNAi was performed in Paramecium by bacteria feeding. Cells exposed to RNAi showed loss of exocytosis (60-70%) after 3-4 generations of growth (72 hr). Using pan-PGM and PFUS antibodies on western blots, PFUS was shown to be specifically knocked-down without obvious change in overall PFUS/PGM level. Immunofluorescent localization in RNAi cells showed reduction of PFUS synthesis and number of DCSVs, indicating PFUS affects the secretory pathway. These cells recover exocytosis capacity ...

A procedure has been developed for the determination of the concentration of infective Newcastle disease virus (NDV) based on the enumeration of singly infected and distributed HeLa cells which are visualized by staining with fluorescent antibody. Infective virus assayed by the fluorescent cell-counting procedure is expressed in terms of cell-infecting units (CIU).. Adsorption of NDV to HeLa cell monolayers reached a plateau 1 to 1.5 hours after inoculation of coverslip cultures, and 12 per cent of the infective particles inoculated failed to adsorb. The half-life of NDV in protein-free Eagles medium at 37°C. was 2.1 hours. There was a linear relationship between virus concentration and the number of infected cells. The coefficient of variation of the mean of replicate determinations of infective NDV was 8.2 per cent. The distribution of single infected HeLa cells in the monolayer corresponded to the Poisson distribution. With NDV the cell-infecting unit (CIU) determined in HeLa cells is ...

The SHV-1 β-lactamase gene was sub-cloned into the pBC SK(-) vector (Stratagene, LaJolla, CA) from a clinical strain of K. pneumoniae (15571), and transformed into E. coli DH10B cells (Invitrogen, Carlsbad, CA) [15]. The K. pneumoniae clinical isolate possessed the SHV-5 ESBL and was obtained from a previous study [16]. E. coli DH10B without the blaSHV-1 gene served as a negative control.. The procedures used to isolate, express and purify the SHV-1 β-lactamase and to produce the anti-SHV β-lactamase antibodies have been previously detailed [13]. Purified anti-SHV antibodies were fluorescein-labeled with the EZ-Label™ fluorescent labeling kit (Pierce, Rockford, IL), according to the instructions of the manufacturer. In brief, 1 mg of polyclonal anti-SHV antibodies in 1 ml phosphate buffered saline (PBS, 2 mM monobasic sodium phosphate, 8 mM dibasic sodium phosphate, 154 mM sodium chloride, pH 7.4) was mixed with 7.6 μl of a 10 mg/ml solution of NHS-fluorescein in N, N-dimethylformamide for ...

Background Hypertonic saline (HS) continues to be successfully utilized clinically for treatment of varied types of cerebral edema. using Traditional western blotting, dual immunofluorescence and real-time RT-PCR, as well as the model also was useful for evaluation of mind drinking water content material (BWC) and infarct size. SB203580 and SP600125, particular inhibitors from the p38 and JNK signaling pathways, had been used to take care of primary microglia ethnicities to determine if the two signaling pathways had been necessary for the inhibition of HS on microglia expressing and secreting TNF- and IL-1 using Traditional western blotting, dual immunofluorescence and enzyme-linked immunosorbent assay (ELISA). The result of TNF- and IL-1 on NKCC1 manifestation in major astrocyte ethnicities was determined. Furthermore, the immediate inhibitory aftereffect of HS on NKCC1 manifestation in major astrocytes was also looked into by Traditional western blotting, dual immunofluorescence and real-time ...

Single-cell analysis reveals aspects of cellular physiology not evident from population-based studies, particularly in the case of highly multiplexed methods such as mass cytometry (CyTOF) able to correlate the levels of multiple signaling, differentiation and cell fate markers. Immunofluorescence (IF) microscopy adds information on cell morphology and the microenvironment that are not obtained using flow-based techniques, but the multiplicity of conventional IF is limited. This has motivated development of imaging methods that require specialized instrumentation, exotic reagents or proprietary protocols that are difficult to reproduce in most laboratories. Here we report a public-domain method for achieving high multiplicity single-cell IF using cyclic immunofluorescence (CycIF), a simple and versatile procedure in which four-color staining alternates with chemical inactivation of fluorophores to progressively build a multichannel image. Because CycIF uses standard reagents and instrumentation ...

Summary Fluorescent antibody technics were used to determine the localization and distribution of Schistosoma mansoni antigen in tissue cells, the presence of circulating antibody, and the sites of antibody production or in vivo antigen-antibody combination. The experiments were performed in mice after a primary infection with cercariae, after several challenges, and after antigen stimulation and challenge. Evidence of the presence of circulating antibody was first observed at 20 days in the inhibition fluorescent antibody test, at 25 days in the indirect fluorescent antibody test, at 42 days with the cercarial fluorescent antibody test, and at 47 days in the Cercarienhullen reaction. Sites of antibody production or in vivo antigen-antibody combination were observed in inflammatory cells of the portal tracts, especially those along the smaller arteries, and in granulomas and isolated cells in the parenchyma of the liver; in the perivascular tissue cells, isolated parenchymal cells and granulomas of the

Synonyms for direct fluorescein-conjugated antibody in Free Thesaurus. Antonyms for direct fluorescein-conjugated antibody. 21 words related to antibody: active site, protein, autoantibody, precipitin, ABO antibodies, Rh antibody, antitoxin, agglutinin, Forssman antibody.... What are synonyms for direct fluorescein-conjugated antibody?

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BioAssay record AID 332145 submitted by ChEMBL: Induction of cellular microtubule disrupting activity in rat A10 cells at 1 ug/ml after 24 hrs by indirect immunofluorescence technique.

We wished to determine whether CD4+ T cells could reject a skin graft that was discordant for a single minor transplantation Ag in the absence of CD8+ T cells or Ab. Transgenic A1(M) mice were constructed that express the rearranged V beta 8.2 and V alpha 10 TCR genes from a T cell clone that is specific for the male Ag (H-Y) in the context of H2-Ek. In addition, the RAG-1(-/-) background was bred onto these mice to eliminate any endogenous TCR rearrangements. As expected, clonal deletion was found to be complete in the thymus of male A1(M) x RAG-1(-/-) mice, while only CD4+ T cells were positively selected and found in the periphery of females. Female A1(M) x RAG-1(-/-) mice were able to rapidly reject (in |14 days) male (but not female) skin grafts in a CD4-dependent fashion. After multiple grafts, it was confirmed that no CD8+ T cells or surface Ig+ B cells were present. An immunofluorescent analysis of spleen cells after grafting showed that the majority of T cells expressed activation markers (CD44

In the present study, we examined AQP4 function in reactive astrocytes compared between wild-type (WT) and AQP4-deficient (AQP4/KO) mice after a stab wound to the cerebral cortex. To examine activity of astrocytes, proliferating cells were labeled with 5-bromo-2-deoxyuridine (BrdU) incorporated in the drinking water provided to mice. By the immunofluorescent analysis using anti-BrdU antibody, astrocyte reactivity was at high level around the lesion site for WT mice 3 days after the stab wound to the brain, while it was much less for AQP4/KO mice. To identify the molecules related in injured mouse brain, we performed microarray analysis and found that more than 400 genes around the lesion site were upregulated 3 days after the wounding in WT mice ...

Efficient cleavage and probe versatility for highly-multiplex detection and co-localization. Modified Lightning Terminators™ provide a robust method for detecting multiple antigens from a single sample. Immunofluorescent detection of antigens in fixed tissue specimens and on immunoblots is used routinely in clinical practice and research laboratories, but current methods are limited to the detection of one to four antigens per tissue section or blot. Consequently, the detection of additional antigens requires multiple independent stains on separate sections and limits the co-localization of antigens on a single section. Whether in immunohistochemistry or immunoblotting, our photocleavable fluorescent labels (PCLs) provide advantages over current methods.. Highly Serial and Multiplex Detection. PCL technology uses our expertise in photocleavable chemistry to provide structures that can both efficiently bind to target antigens and rapidly cleave the dye reporter after exposure to UV light. The ...

Animals, Antilymphocyte Serum, Cell Separation, Complement System Proteins, Fluorescent Antibody Technique, Genetic Linkage, Isoantigens, Killer Cells, Natural, Male, Mice, Mice, Inbred A, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Inbred DBA, Mice, Inbred NZB. ...

Recent investigations have shown the feasibility of applying the fluorescent antibody method to Lancefield grouping of streptococci.1-4 The present study was un

Buy Goat Anti-Rabbit IgG (H+L chain specific) secondary antibody (MBS674759) product datasheet at MyBioSource, Secondary Antibodies. Application: ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry

Cy3 Conjugated Anti-Mouse IgG SABC Kit is for indirect sensitive immunofluorescent detection of proteins in ICC or IHC via StreptAvidin-Biotin Complex (SABC) method.

Confocal immunofluorescent detection of wingless protein (green), engrailed protein (blue), and enhancer trap 5953 (red) in a reiterated segment of embryonic Drosophila neuroectoderm (ventral midline, center; anterior, top). The wingless protein nonautonomously activates 5953 expression, maintains expression of the engrailed protein, and controls the formation and specification of central nervous system precursors. See page 1594. [Photo: Chris Q. Doe] ...

Background Bcl-2 (B cell lymphoma/leukemia gene-2) may be the 1st proto-oncogene proven to function by inhibiting programmed cell loss of life/apoptosis. immunofluoresence and blot staining were employed to testify the marker protein of both mesenchymal and epithelial cells. Outcomes Unexpectedly, a dramatic modification of phenotype from epithelial cells to fibroblast-like cells was seen in Bcl-2-transfected cells. Traditional western blot immunofluoresence and evaluation MS-275 staining outcomes proven how the E-cadherin and desmoplakin, markers of epithelial cells, had MS-275 been downregulated in the Bcl-2-transfected cells. Nevertheless, Vimentin and N-cadherin, markers of mesenchymal cells, had been upregulated in these cells. Redistributions of cytokeratin and beta-catenin were seen in the Bcl-2-transfected cells also. Our outcomes demonstrated how the Bcl-2-transfected MCF10ATG3B cells maintained some epithelial markers additional, such as for example epithelial particular antigen (ESA) ...

annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...

[button size=small text=MSDS & Datasheet link=/wp-content/uploads/media/BCDatasheets_C_10.26/AGK/AGK-007-2.pdf]Pure Goat Anti-Rabbit IgG Gel, Spe

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Main Page , Multiple Labeling. It is often useful to be able to stain for two or more antigens in one common tissue section. This can be achieved by immunofluorescence method using different fluorescent dyes. Multiple staining can also be done with peroxidase conjugated antibodies developed with different chromogen substrates to produce the end products of different colors. There are three basic approaches in planning multiple staining: parallel, sequential and adjacent. In addition, the antibody dilution and condition are also important factors to be considered. Finally, appropriate color combination is also crucial since improper color combination may produce poor result and fail to demonstrate multiple antigens in the same section. For best result, the careful design and test of multiple staining protocols are necessary.. ...

The Gibbs laboratory is focused on the development of novel fluorophores and fluorescence imaging technologies to improve cancer detection and treatment. The main research focuses of the group are on fluorophore development for image-guided surgery, fluorescent labeling of small molecule therapeutics to improve understanding of effective cancer therapy and development of highly multiplexed immunofluorescence imaging technologies to permit deep multiplexed imaging on tissues. Current projects in the Gibbs laboratory include (1) development of near infrared nerve-specific fluorophore to improve nerve sparing during radical prostatectomy, (2) contrast agent and imaging methodology development to improve breast cancer margin assessment in the operating room, (3) design and synthesis of fluorescently labeled small molecule therapeutics for personalized therapy prediction in cancer, and (4) development of improved cyclic immunofluorescence methods to permit up to 50 color staining on a single tissue ...

Lab Tests Online - UK is a website written by practising laboratory doctors and scientists to help you understand the many clinical laboratory tests that are used in diagnosis, monitoring and treatment of disease ...

productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet ...

The past couple months, the media has shined light on how dangerous COVID-19 can be, but it seems they have failed to mention the latest developments and simply preyed on the fear of Americans.. Looking back to March 3, WHO said the coronavirus death rate was 3.4%, around 34 times more lethal than that of the seasonal flu. With that given estimate, it was reasonable to close down stores, restaurants and take precautions necessary.. But as more data comes to light with the novel virus, it could be that the projected 3.4% death rate is greatly exaggerated.. A study conducted in early April at Santa Clara county concluded that a fatality rate for the county was around 0.1-0.2%, extremely similar to the seasonal flu. The fatality rate was far lower because of unconfirmed and asymptomatic cases.. However, it is important to not judge a virus fatality rate on just one study. That leads me to an antibody study conducted in New York.. New York is reporting 15,500 COVID-19 deaths, and if 2.7 million ...

HRP Conjugated anti-Rabbit IgG SABC Kit, with the specific secondary antibody and all the reagents needed for performing IHC-P;IHC-F;ICC

Filling a gap in the literature, this atlas stands as a unique authoritative source to thoroughly review the many immunofluorescence patterns seen in immunoderm

iPSc-EGFRvIII. CLTH/iPSc-EGFRvIII cell line is induced pluripotent stem cell line that stably expresses high level of EGF mutant receptor vIII and puromycin-resistant gen introduced by virus transduction. EGFRvIII is under EF1 promoter control, allowing high levels of protein expression. Immunofluorescent studies indicate expression of transcription factors (Sox2, Oct4, Nanog) as well as surface proteins (Tra1-81 and Tra1-60) characteristic for iPS cells.. ...

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