An instrument is disclosed for fluorescence assays which is capable of reading many independent samples at the same time. This instrument provides enhanced throughput relative to single-sample instruments, and is well-suited to use in general fluorescence, time-resolved fluorescence, multi-band fluorescence, fluorescence resonance energy transfer (FRET), and fluorescence polarization. This invention is beneficial in applications such as high-throughput drug screening, and automated clinical testing. Also disclosed are means and methods for a fluorescence polarization measurement which is highly sensitive, inherently self-calibrated, and unaffected by lamp flicker or photobleaching. This fluorescence polarization invention can be practiced on a variety of fluorescence instruments, including prior-art equipment such as microscopes and multi-well plate readers.
A time-resolved fluorescence anisotropy imaging method for studying nanoscale clustering of proteins or lipids was developed and evaluated. It is based on FRET between the identical fluorophores (homo-FRET), which results in a rapid depolarization of the fluorescence. The method employs the time-resolved fluorescence anisotropy decays recorded in a confocal microscope equipped with pulsed excitation and time-gated detection. From the decay the limiting anisotropy r inf was derived, which is a direct measure for the number of fluorophores per cluster. The method was evaluated by imaging GPI-GFP, a lipid raft marker. Small clusters were observed in the plasma membrane while the cytoplasm and the Golgi contained predominantly monomers.. ©2007 Optical Society of America. Full Article , PDF Article ...
Using a confocal epi-illuminated microscope with a polarizing beam splitter and dual-channel detection of single-molecule fluorescence induced by pulsed laser excitation, a new application of the three-dimensional, real-time spectroscopic technique BIFL (burst integrated fluorescence lifetime) is introduced. BIFL allows simultaneous registration of fluorescence intensity, lifetime, and anisotropy. It is shown to be well-suited to identify the freely diffusing fluorescent molecule Rhodamine 123 and the Enhanced Yellow Fluorescent Protein via their characteristic fluorescence anisotropy using a time-resolved analysis. Furthermore, data analysis is discussed and rotational correlation times of single molecules are determined. Applications for multidimensional single-molecule identification are outlined.
Fluorescence polarization assays (FP assays) can be used in a variety of ways. FP assays are a great fit for HTS because the assays are simple & homogenous.
Fluorescence Polarization Assay for the Diagnosis of Anti-Brucella abortus Antibodies in Cattle Serum: Adaptation for its Use in Microplates and Comparison with Conventio..
Vol 3: Screening for Antifibrotic Compounds Using High Throughput System Based on Fluorescence Polarization.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
DO NOT REPLY TO THIS MESSAGE DIRECTLY PLEASE VISIT http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html New on ExpMed Job Listings (Post Doc: fluorescence polarization assays (FPA) for the diagnosis of Infectious Disease Min Lin, Ph.D. Animal Diseases Research Institute Immunology Nepean, Ontario, Canada Project description: A post-doctoral position is available immediately to carry out research that leads to development of homogeneous fluorescence polarization assays (FPA) for the diagnosis of infectious diseases. The studies may include identification and characterization of the immunodominant protein/carbohydrate antigens from pathogenic bacteria, molecular cloning, protein expression/purification, epitope mapping, chemical modification of proteins, structure/function analysis of proteins, and assessment of the immune response to antigens. Recent publications: (1)Lin, M., and K. Nielsen. 1997. Binding of the Brucella abortus lipopolysaccharide O-chain fragment to a monoclonal antibody. J. Biol. ...
Fluorescence polarization measurements are made using an optical system that includes polarizing filters in the light path. Samples in the microplate are excited using polarized light, and depending on the mobility of the fluorescent molecules found in the wells, the light emitted will either be polarized or not.
Currently, the most used methods for genotyping of SNPs are costly and time consuming. It is necessary to develop a method that is more accurate, (...)
Fluorescence polarization (FP) is a sensitive, robust, and homogeneous assay format, able to probe a diversity of biological molecules and their interactions. Herein, we describe a new FP strategy based on the use of streptavidin as a signal amplifier. Such signal amplified fluorescence polarization (SAFP) w
We report on various excitation geometries used in ultrasensitive flow cytometry that yield a linear relation between the fluorescence intensity measured from individual stained DNA fragments and the lengths of the fragments (in base pairs). This linearity holds for DNA samples that exhibit a wide range of conformations. The variety of DNA conformations leads to a distribution of dipole moment orientations for the dye molecules intercalated into the DNA. It is consequently important to use an excitation geometry such that all dye molecules are detected with similar efficiency. To estimate the conformation and the extent of elongation of the stained fragments in the flow, fluorescence polarization anisotropy and autocorrelation measurements were performed. Significant extension was observed for DNA fragments under the flow conditions frequently used for DNA fragment sizing. Classical calculations of the fluorescence emission collected over a finite solid angle are in agreement with the ...
Inhibition at 50 ug/ml (%) by fluorescein-FtsZpeptide fluorescence polarization (FP) competition assay. It is interesting that the fluorescence polarization assay was not able to detect the weak binding to ZipA for many of the analogs for which the HSQC (NMR) experiment unambiguously showed specific binding to ...
Fluorescence polarization (FP) is sensitive to changes in molecular size and, therefore, assays that make use of molecular interactions are particularly useful. FP immunoassays, which rely on antibody-antigen interactions are widely used for clinical analysis, food analysis, and environmental monitoring. Development of other FP techniques, such as receptor-ligand and peptide-ligand affinity assays, aptamer affinity assays, immobilized metal assays for phosphochemicals, assays for single nucleotide polymorphisms, and capillary electrophoresis laser-induced FP assays, have further extended the applications of FP techniques to drug discovery, protein-DNA interactions, disease diagnostics, and biochemical research. The use of microplate readers equipped with polarizing optics has led to the adoption of FP as a readout mode for high-throughput screening assays. This chapter briefly describes the basic principle of FP and summarizes some of the recent bioanalytical applications of FP. It also discusses the
High moisture extrusion using twin‐screw extruders shows great promise for producing meat analog products with vegetable proteins. The resulting products have well‐defined fiber formations and resemble real meat in both visual appearance and taste sensation. Developing reliable nondestructive techniques to quantify the textural properties of extrudates is important for quality control in the manufacturing process. In this study, we developed an image‐processing technique to automatically characterize sample fiber formation using digital imaging. The algorithm is based on statistical analysis of Hough transform. This objective method can be used as a standard method for evaluating other noninvasive methods. We have compared the fiber formation indices measured using this technique and a noninvasive fluorescence polarization method and obtained a high correlation.. ...
BioAssay record AID 651798 submitted by Broad Institute: Fluorescence polarization to screen for inhibitors that disrupt the protein-protein interaction between Keap1 and Nrf2 Measured in Biochemical System Using Plate Reader - 2119-01_Inhibitor_Dose_DryPowder_Activity.
Drugs are important in curing diseases, and in the latter decade, research in the use of drugs to cure cancer has grown exponentially. There are thousands of drug candidates that go through screening processes each year, but only a handful reach the clinical stage, and in the end get patented for use in treatment of diseases. One of the major difficulties today is to know if the drug binds to the desired target. Identifying the target is termed drug target deconvolution. Existing drug target deconvolution strategies have limitations, hence there is a need for a new/complementary methodology, with a high degree of throughput, versaitility and reliability. We propose a method which combines liquid chromatography (LC) in three orthogonal dimensions together with fluorescence polarization (FP) and mass spectrometry (MS) to identify target proteins in complex mixtures. Results show that FP applied on fluorecein-tagged drugs is a robust, sensitive and spesific technique to identify protein-drug ...
Crystallographic and solution studies have shown that IgE molecules are acutely bent in their Fc region. cross-linking by allergen leads to cell degranulation, release of inflammatory mediators and an immediate allergic response. Disruption of the IgE-FcRI conversation is usually a validated strategy for therapeutic intervention in allergic diseases including asthma: an anti-IgE monoclonal IgG antibody, omalizumab (Xolair?, Novartis Pharmaceuticals Ltd), inhibits IgE binding to FcRI and is effective in the Tarafenacin treatment of severe persistent asthma and other allergic diseases2. IgE consists of a dimer of two identical heavy and two identical light chains, but unlike IgG in which the antigen-binding Fab region is separated from the receptor-binding Fc region by a flexible hinge, IgE contains an additional disulphide-linked pair of domains, (C2)2, forming a (C2-C3-C4)2 dimer1. Fluorescence depolarisation studies to assess segmental flexibility have shown IgE to be less flexible than IgG3-6, ...
... is an amino dye for TAMRA channel. The dye possesses bright fluorescence and is well suitable for the fluorescence polarization assays.
NVP-BEP800 is a fully synthetic, orally bioavailable inhibitor of Hsp90 with IC50 value of 58nM [1].NVP-BEP800 binds to the N-terminal ATP-binding pocket of Hsp90. In a competitive binding fluorescence polarization assay, NVP-BEP800 inhibits Hsp90β with I
This Application Note demonstrates how the Predictor hERG fluorescence polarization assay can be measured on a Plate Reader. Read more.
Sigma-Aldrich offers abstracts and full-text articles by [Lily Mahapatra, Chengjian Mao, Neal Andruska, Chen Zhang, David J Shapiro].
As PARP-1 inhibitors continue to hold tremendous promise as targeted anticancer agents, there is a growing interest in specific PARP-1 inhibitors. The unique structural aspects of the PARP-1 complex of essential domains is therefore of particular interest as a therapeutic target. Using the core components of this complex as our target model (Fig. 4A), we developed a high-throughput assay that is capable of detecting complex formations in the presence of DNA. The assay is based on fluorescent polarization techniques and detects the binding of WGR-CAT to a Zn1-Zn3 saturated DNA complex as an increase in fluorescence polarization (Fig. 4B). To validate that the readout accurately represented allosteric activation, we tested several mutants at the domain interfaces. Four mutations were made along the Zn1-Zn3 interface: E76A, R78A, W79A, and N242W; two mutations were made along the Zn3-WGR-HD interface: W318R and W318A. Mutations in the Zn1-Zn3 construct did not affect their DNA-binding affinity ...
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ADC_Keywords: Molecular clouds ; Polarization Keywords: catalogs - ISM: clouds - ISM: magnetic fields - polarization - ISM: individual (Chamaeleon-Musca Dark Cloud Complex) - Abstract: We have used CCD imaging polarimetry to obtain linear polarization measurements of background stars toward the filamentary Musca dark cloud. We present a catalog of 2497 objects with polarization signal-to-noise ratio larger than 5. This allows us to build polarization maps to infer the detailed geometry of the local magnetic field. We show composite polarization maps along the cloud and explore general correlations of the polarimetric data with the morphology of the region. We find the overall field to be strikingly aligned with the projected small axis of the filamentary cloud. We detect a lower limit for the polarization across the cloud of ∼2%, with an enhanced polarization of 6%-7% in the central region. We find evidence that the polarization pattern is altered in the inner regions, those associated with ...
Cosmic microwave background (CMB) polarization measurements can give us extremely valuable information about our universe. Measurements of these faint signals will play a major role in understanding the inflationary epoch and the distribution of matter and the evolution of large scale structure. Arrays of thousands of background-limited detectors are required. Our approach involves Kinetic Inductance Detectors (KIDs), which use small superconducting resonant circuits to detect incident photons (P.K. Day, Nature (2003)). The photons are absorbed, breaking Cooper pairs and causing a change in the kinetic inductance which results in a shift in the resonant frequency of the circuit. KIDs offer significant advantages over competitor detectors in terms of multiplexing, fabrication, and critical temperature tolerance ...
The oligomeric state and activation mechanism that enable p75 NTR to mediate these effects have recently been called into question. In this new study, we have investigated mutant mice lacking the p75NTR death domain (DD) or a highly conserved transmembrane (TM) cysteine residue (Cys 259) implicated in receptor dimerization and activation. Neuronal death induced by proneurotrophins or epileptic seizures was assessed and compared with responses in p75NTR knock-out mice and wild-type animals. Proneurotrophins induced apoptosis of culturedhippocampalandcorticalneuronsfromwild-typemice,butmutantneuronslackingp75NTR,onlythep75NTR DD,orjustCys259 were all equally resistant to proneurotrophin-induced neuronal death. Homo-FRET anisotropy experiments demonstrated that both NGF and proNGF induce conformational changes in p75 NTR that are dependent on the TM cysteine. In vivo, neuronal death induced by pilocarpine-mediated seizures was significantly reduced in the hippocampus and somatosensory, piriform, ...
Title: Antibody-based Fluorescence Polarization Assay to Screen Combinatorial Libraries for Sweet Taste Compounds. VOLUME: 4 ISSUE: 5. Author(s):D. S. Linthicum, J. Patel and N. Cairns. Affiliation:Delaware Water Gap Science Institute, 399 Autumn Dr., Bangor, PA 18013, Tel: 570-897-0284.. Keywords:antibody based fluorescence polarization assay, immunoassay, fluorescence polarization immunoassay fpia, fluorescence polarization immunoassay, high throughput screening hts. Abstract: Technological advances in instrumentation, chemical synthesis methods, molecular biology and biochemistry have fueled the recent growth in high throughput screening. Assays are available in a vast range for formats, including fluorescence, luminescence, absorbance, and scintillation detection. Antibodies represent a powerful tool for novel compound discovery and their utility in this regard should not be underestimated. We have designed a fluorescence polarization immunoassay for the identification of novel sweeteners. ...
PAULIN, Lília Márcia Silva et al. Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C) and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis). Cienc. Rural [online]. 2012, vol.42, n.9, pp.1621-1626. Epub 14-Ago-2012. ISSN 1678-4596. http://dx.doi.org/10.1590/S0103-84782012005000070.. The objective of the present study was to compare the performance of three serological tests for diagnosis of Brucella abortus infections in buffaloes (Bubalus bubalis). Serum samples collected from 696 adult females were submitted to the competitive enzyme-linked immunosorbent assay (ELISA-C), (I-ELISA), fluorescence polarization test (FPA), 2-mercaptoethanol test (2-ME) and complement fixation test (CFT). The gold standard was the combination of CFT and 2-ME, considering as positive the reactors in both CFT and 2-ME, and as negative those non-reactors. ROC analyses were done for C-ELISA, I-ELISA and FPA and the Kappa agreement index were also ...
Species, Scientific Experts, Publications, Genomes and Genes, Research Grants, Research Topics about fluorescence polarization immunoassay
0163]Although the invention has been described with reference to the examples provided above, it should be understood that various modifications can be made without departing from the spirit of the invention. Accordingly, the invention is limited only by the following claims. Sequence CWU 1 SEQUENCE LISTING ,160, NUMBER OF SEQ ID NOS: 96 ,210, SEQ ID NO 1 ,211, LENGTH: 206 ,212, TYPE: PRT ,213, ORGANISM: Homo sapiens ,400, SEQUENCE: 1 Met Ala Glu Asp Ala Asp Met Arg Asn Glu Leu Glu Glu Met Gln Arg 1 5 10 15 Arg Ala Asp Gln Leu Ala Asp Glu Ser Leu Glu Ser Thr Arg Arg Met 20 25 30 Leu Gln Leu Val Glu Glu Ser Lys Asp Ala Gly Ile Arg Thr Leu Val 35 40 45 Met Leu Asp Glu Gln Gly Glu Gln Leu Glu Arg Ile Glu Glu Gly Met 50 55 60 Asp Gln Ile Asn Lys Asp Met Lys Glu Ala Glu Lys Asn Leu Thr Asp 65 70 75 80 Leu Gly Lys Phe Cys Gly Leu Cys Val Cys Pro Cys Asn Lys Leu Lys 85 90 95 Ser Ser Asp Ala Tyr Lys Lys Ala Trp Gly Asn Asn Gln Asp Gly Val 100 105 110 Val Ala Ser Gln Pro Ala Arg Val Val Asp Glu Arg Glu ...
BioAssay record AID 749488 submitted by ChEMBL: Inhibition of p53/MDM2 (1 to 118) (unknown origin) interaction at 10 uM after 1 hr by fluorescence polarization assay.
ICUMSA Method GS 4/7-1 (2013)The Determination of Apparent Sucrose in Molasses by a Double Polarisation Method Single Method, available as online version (2 years) on www.icumsa.org ...
Crystallographic and solution studies have shown that IgE molecules are acutely bent in their Fc region. cross-linking by allergen leads to cell degranulation, release of inflammatory mediators and an immediate allergic response. Disruption of the IgE-FcRI conversation is usually a validated strategy for therapeutic intervention in allergic diseases including asthma: an anti-IgE monoclonal IgG antibody, omalizumab (Xolair?, Novartis Pharmaceuticals Ltd), inhibits IgE binding to FcRI and is effective in the Tarafenacin treatment of severe persistent asthma and other allergic diseases2. IgE consists of a dimer of two identical heavy and two identical light chains, but unlike IgG in which the antigen-binding Fab region is separated from the receptor-binding Fc region by a flexible hinge, IgE contains an additional disulphide-linked pair of domains, (C2)2, forming a (C2-C3-C4)2 dimer1. Fluorescence depolarisation studies to assess segmental flexibility have shown IgE to be less flexible than IgG3-6, ...
The binding of interleukin-8 (IL-8) to heparan sulfate (HS) proteoglycans on the surface of endothelial cells is crucial for the recruitment of neutrophils to an inflammatory site. Fluorescence anisotropy measurements yielded an IL-8 dimerization constant of 120 nM. The binding affinities, obtained by isothermal fluorescence titration, of size-defined heparin and HS oligosaccharides to the chemokine were found to depend on the oligomerization state of IL-8: high affinity was detected for monomeric and low affinity was detected for dimeric IL-8, referring to a self-regulatory mechanism for its chemoattractant effect. The highest affinity for monomeric IL-8 was detected for the HS octamer with a Kd , 5 nM whereas the dissociation constants of dimeric IL-8 were found in the medium micromolar range. No indication for increasing affinities for monomeric IL-8 with increasing oligosaccharide chain length was found. Instead, a periodic pattern was obtained for the dissociation constants of the GAG ...
MDM2 and X-linked inhibitor of apoptosis (XIAP) promote cancer cell survival by binding p53 and promoting its degradation and by inhibiting caspase activation to prevent apoptosis, respectively. Further, the RING domain of MDM2 can bind to the internal ribosome entry site (IRES) of XIAP mRNA transcripts to promote XIAP translation, increase MDM2 protein expression, and enhance resistance to apoptosis, suggesting a potential p53-independent role for MDM2 in enhancing cell survival. Gu and colleagues hypothesized that disrupting the interaction between MDM2 and XIAP would decrease expression of both proteins and enhance cancer cell apoptosis. A fluorescence polarization assay was developed for high-throughput screening of small-molecule inhibitors of XIAP IRES binding to the MDM2 RING domain. Of 141,394 small-molecule compounds tested, 8 candidates disrupted MDM2-XIAP binding, and 3 compounds selected for further study (MX3, MX11, and MX69) reduced protein expression of both MDM2 and XIAP when ...
Abstract: Precision polarimetry is essential for future e+ e- colliders and requires Compton polarimeters designed for negligible statistical uncertainties. In this paper, we discuss the design and construction of a quartz Cherenkov detector for such Compton polarimeters. The detector concept has been developed with regard to the main systematic uncertainties of the polarisation measurements, namely the linearity of the detector response and detector alignment. Simulation studies presented here imply that the light yield reachable by using quartz as Cherenkov medium allows to resolve in the Cherenkov photon spectra individual peaks corresponding to different numbers of Compton electrons. The benefits of the application of a detector with such single-peak resolution to the polarisation measurement are shown for the example of the upstream polarimeters foreseen at the International Linear Collider. Results of a first testbeam campaign with a four-channel prototype confirming simulation predictions ...
!%Axometrics Inc.%! has introduced two options for its AxoScan family of polarimeters. The out-of-plane measurement fixture rotates and tilts a sample
Los Angeles, CA (PRWEB) March 7, 2011 -- General Photonics, a market leader in polarization control and polarization measurement, today announced a complete
Recently, Hovatta et al. (2012) showed that one can measure the transverse rotation gradient in the jet of 3C 273. 2 Synchrotron Emission field enclosing and collimating the jet. Because the rotation affects the high frequencies the least, the best way to get an estimate of the intrinsic polarization of a synchrotron source is to measure at high frequencies. This makes X-ray polarization measurements an interesting prospect. Figure courtesy of Simona Soldi. Here K5/3 is the modified Bessel function of order 5/3. 3 where F is presented as a function of frequency in units of the critical frequency. 1 Synchrotron Emission of a Particle Plasma In astrophysical sources we observe emission from large numbers of charged particles. If we consider for example an electron plasma emitting synchrotron radiation, we can simply sum up the single particle contributions. This emission can then be absorbed again before leaving the plasma. 2008) or rotation of the narrow-line region about the central black ...
Cosmic microwave background (CMB) polarization measurements can give us extremely valuable information about our universe. Measurements of these faint signals will play a major role in understanding the inflationary epoch and the distribution of matter and the evolution of large scale structure. Measuring the CMB polarization has become one of the major goals of CMB experiments. However, the polarized CMB signal is so small that its measurement requires not only very high instrumental sensitivity, but also exquisite control of systematics. In collaboration with Greg Tuckers group at Brown University, Ben Wandelts group at the Institut dAstrophysique (Paris) and Ted Bunns group at University of Richmond, we are building a suite of simulation software to simulate CMB interferometric observations and to assess various systematic errors in CMB power spectra estimates by using maximum likelihood method and Gibbs sampling technique ...
A magnetic material is evaporated on a transparent substrate. The magnetic fields are all aligned in the same direction along the tracks.. The media is read by observing the rotation of the polarization of the NSOM signal.. Bits are written by increasing the power input into the fiber probe.. The tip of the probe heats up, and transfers energy to the sample locally. This raises the temperature of the magnetic film above the Curie temperature, and the direction of the field in the local region reverses (driven by the magnetic field from the neighboring areas).. Read time is thus limited by the signal to noise for polarization detection (can be influenced by the topography).. Write time is limited by the thermal transfer process.. Seek time is limited by the speed of the feedback system to maintain the position of the probe in the near-field while avoiding a crash.. Long term reliability is probably determined by the probe life, which is currently quite short even without the rapid raster ...
The oligomeric state and activation mechanism that enable p75 NTR to mediate these effects have recently been called into question. In this new study, we have investigated mutant mice lacking the p75NTR death domain (DD) or a highly conserved transmembrane (TM) cysteine residue (Cys 259) implicated in receptor dimerization and activation. Neuronal death induced by proneurotrophins or epileptic seizures was assessed and compared with responses in p75NTR knock-out mice and wild-type animals. Proneurotrophins induced apoptosis of cultured hippocampal and cortical neurons from wild-type mice, but mutant neurons lacking p75NTR, only the p75NTR DD, or just Cys259 were all equally resistant to proneurotrophin-induced neuronal death. Homo-FRET anisotropy experiments demonstrated that both NGF and proNGF induce conformational changes in p75 NTR that are dependent on the TM cysteine. In vivo, neuronal death induced by pilocarpine-mediated seizures was significantly reduced in the hippocampus and ...
단백질 상호 작용은 세포의 기능의 핵심이다. 열량 및 분광 기술은 일반적으로 그 특성을하는 데 사용됩니다. 여기에서 우리는 Shwachman - 다이아몬드 증후군 (SBDS)에 돌연변이 단백질과 신장 인자 같은 1는 GTPase (EFL1) ...
Figure S4. 6-hydroxyindole transition bandshapes, 1La and 1Lb, calculated from eqns. (6) and (7), using limiting anisotropy values derived from the fluorescence excitation anisotropy measurement at 77 K, given in Fig. 4b: fa(λ), dashed line and fb(λ), solid line ...
Abstract. Instruments dedicated to aerosol monitoring are recently available and the POLDER (POLarization and Directionality of the Earths Reflectances) instrument on board the PARASOL (Polarization & Anisotropy of Reflectances for Atmospheric Sciences coupled with Observations from a Lidar) mission is one of them. By measuring the spectral, angular and polarization properties of the radiance at the top of the atmosphere, in coordination with the other A-Train instruments, PARASOL provides the aerosol optical depths (AOD) as well as several optical and microphysical aerosol properties. The instrument, the inversion schemes and the list of aerosol parameters are described. Examples of retrieved aerosol parameters are provided as well as innovative approaches and further inversion techniques. ...
Functional effect of RNA and ssDNA on the SERCA/PLN complex.(a) Dissociation constants as measured by both fluorescence polarization and affinity capillary elec
In order to find compounds that can detach the PSD-95 protein from the NMDA receptor, one must have a method for measuring the extent to which the compounds bind to PSD-95.. "We have established a method - fluorescence polarisation - that has been very successful in helping us develop a number of potent compounds over a long period of time," recounts Anders Bach. He adds that scientists have committed many resources to finding out exactly how the compounds bind to PSD-95 - using for example sophisticated biophysical methods. This has resulted in detailed understanding of the molecular mechanisms that cause the high level of activity.. Anders Bach hopes that the new compound can form the basis for a new drug on the global market, but he emphasises that such a process is long and complicated:. "Although we are very satisfied with the new results in terms of the possible treatment of brain damage due to stroke, many things can go wrong in the long drug development process. So even though the ...
The POLARstar Omega is the fully-equipped Omega-series microplate reader, offering all the features of a FLUOstar Omega, and in addition fluorescence polarization. Equipped with BMG LABTECHs ultra-fast UV/Vis spectrometer and Simultaneous Dual Emission detection, the POLARstar Omega represents a great combination of performance and flexibility for life science applications ...
), ElsevierNorth-Holland Biomedical Press, Amsterdam, pp. There has been considerable reported speculation as to the cause of her death. Prрpranolol, frequent GI and central nervous side effects limit the dose and possible therapeutic benefit. e.
Fluorescence polarization immunoassays (FPIA) were developed for the determination of pesticides triazophos and carbaryl in wheat grains using the portable FPIA device Sentry 200 (Ellie). FPIA of carbaryl was developed first. Tracers for FPIA were synthesized and their structures were confirmed via mass spectrometr