Cell fusion is known to underlie key developmental processes in humans and is postulated to contribute to tissue maintenance and even carcinogenesis. The mechanistic details of cell fusion, especially between different cell types, have been difficult
The final biological events in the life of a worm are described today, revealing how death spreads like a wave from cell to cell until the whole organism is dead.
The X-axis is the amount of red fluorescence. The more red fluorescence a cell emits, the farther to the right the cell data will appear on the histogram. The Y-axis is the amount of blue fluorescence. The more blue fluorescence a cell emits, the cell data will appear closer to the top on the histogram. Remember, CTLs have a high level of protein B(blue) and protein R (red).. Quadrant 1 shows data for a cells with many blue fluorochromes and no red fluorochromes. Data for cells with high levels of both blue and red fluorochromes will appear in quadrant 2. If cells have neither blue nor red fluorochromes, the data will appear in quadrant 3. Data for a cell with many red fluorochromes and no blue fluorochromes attached will appear in quadrant 4.. ...
A system and method for imaging tissue autofluorescence through a video endoscope is described, comprising a light source capable of providing both ultraviolet light capable of inducing tissue autofluorescence and visible light which induces little or no autofluorescence, an optical system to deliver both wavelength bands to the tissue with the same apparent spatial and angular intensity distribution, a means for digitally acquiring the resulting, visible fluorescence and visible reflectance images using a single imaging detector at the distal tip of the endoscope and a means for digitally processing said images to generate a final, false-color image for display which indicates regions of tissue dysplasia. This system can either be added on to an existing video endoscope or integrated into its structure. The combined system can be electronically switched between normal white light imaging and fluorescence imaging.
The AccuClear® and AccuBlue® DNA Quantitation kits are designed for use with fluorescence 96-well plate readers. AccuClear®, AccuBlue® NextGen and AccuBlue® High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). AccuBlue® Broad Range requires an instrument equipped to read blue fluorescence emission (Ex/Em 350/460 nm).. These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor™-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kit, which is designed for use on that instrument.. The AccuGreen™ High Sensitivity DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA High Sensitivity assay, and is a direct replacement for the ...
Purified Fixable Dead Cell Staining Kit (Red Fluorescence) from Creative Biomart. Fixable Dead Cell Staining Kit (Red Fluorescence) can be used for research.
The brightness and stability cerFP505 are similar to other fluorescent proteins used in biomedical research. The fluorescence can be switched on and off, Scope)
Think about fireworks again. You can see them "live", or on a screen, or in a photo. Seeing them live is best, but usually you cant do that and you need to rely on someone filming or photographing. Sometimes the filming or photographing is good, sometimes its not, and so what you see can range from terrific to terrible. Its the same with photographing fluorescence and phosphorescence. Photographing fluorescence is challenging. There are many terrible photos of it around, especially on eBay. Photographing phosphorescence, however, is much more challenging. You have to turn the UV lamps off and immediately open the shutter. A typical setting for my fluorescence photos is [ 1 sec @ f5.6 @ ISO100 ]. A typical setting for my phosphorescence photos is [ 30 sec @ f3.5 @ ISO4000 ]. Based on these settings, the total exposure value for the phosphorescence photo is 3,840 times that for the fluorescence photo.*. The brightness of fluorescence and phosphorescence can vary greatly from rock to rock. One ...
The fully sequenced pCERI is transformed into E.coli BL21 and E.coli Nissle 1917 that are used for the characterization of our regulatory system. The expression efficiency of the genes of interest is characterized by a fluorescence-based assay (Results) Overnight cultures of both recombinant strains are inoculated with single colonies. The overnight cultures are diluted with LB-Amp to an OD of 0.02. 200 μ of the dilutions are pipetted in microtiter plates and incubated at 37 °C in the plate reader. Every 15 minutes mRFP fluorescence (Ex. 584 nm, Em. 607 nm), CFP fluorescence (Ex. 439 nm, Em. 476) and OD600 is measured over a period of 13 h. The measured RFU (relative fluorescence unit) is either blotted against time or against OD600 to evaluate the expression behavior of pCERI. Additionally several samples are either induced with 0.1 -50 nM of 3OC6-HSL and 0.1-100 nM of C8-HSL to explore putative advantages of higher HSL-synthesis, that might be possible by positioning each HSL-synthase, ...
A nonfluorescent coumarin-malonitrile conjugate (1) was transformed into a strongly fluorescent molecule through the Michael addition of a thiol group to the α,β-unsaturated malonitrile of 1. The molecular probe has exhibited a highly selective fluorescence response toward biothiols (Cys, Hcy, GSH) with micr
Article Introduction to Fluorescence for Oil in Water Monitoring. Fluorescence can be found in many applications throughout our day-to-day routines. Commercially, one of the most obvious uses is in fluorescent lighting. For this, fluorescence is us...
Background autofluorescence of biological samples often complicates fluorescence-based imaging techniques, especially in aged human...
I perform immunophenotyping of human lymphocytes by flow cytometry. I use 1% paraformaldehyde solution (PFA; Sigma) in PBS for cell fixation. The autofluorescence of fixed cell exited at 488nm is increased 6-7 fold comparing to nonfixed control. 1. Can someone suggest me an alternative PFA source which gives minimal autofluorescence? 2. Can someone suggest me a post-fixation protocol which reduces PFA-fixed cell autofluorescence? Thank you. Dr. L. Volkov ...
The use of "flat" focusing devices, such as Fresnel lenses and holographic optical elements, for the passive optical separation of fluorescence and scattered light is evaluated theoretically and experimentally. Although "flat" lenses do not focus incident light isochronically, and should therefore have different spatial focusing characteristics for very short pulses (scattered light) and pseudo-continuous-wave signals (fluorescence), the optical quality of the flat lenses tested is insufficient to achieve such discrimination. Additionally, if the coherence length of the fluorescence is less than the difference in optical pathlength from extreme positions on the flat lens, fluorescence cannot be considered to be a pseudo-cw signal; this limitation constrains the potential applications of such instrumentation.. PDF Article ...
This fluorescence image gallery explores over 30 of the most common cell lines, labeled with a variety of fluorophores using both traditional staining methods as well as immunofluorescence techniques. This fluorescence image gallery explores over 30 of the most common cell lines, labeled with a variety of fluorophores using both traditional staining methods as well as immunofluorescence techniques.
The compact and power-friendly design of NIGHTSEAs Stereo Microscope Fluorescence Adapter allows you to take it anywhere! Watch this video of fluorescence microscopy at a rocky cove.
Watch this video to learn about the NIGHTSEA Model SFA Stereo Microscope Fluorescence Adapter system, designed to add fluorescence to any stereo microscope at a fraction of what you might expect to pay. Discover how this complete modular system provides everything you need to go from zero to fluorescence in under 60 seconds.
Absorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore)
Press release - business new - Global Fluorescence Lifetime Imaging Microscopy Market 2018 - Leica, Olympus - published on openPR.com
Book Allergy Cephalosporin Fluorescence Assay Blood @Home at Best Prices at the slot of your choice. View details of test: When to take, What is the normal range & Get reports Online.
Lumenera Research grade Peltier cooled CCD digital microscope cameras for scientific, laboratory, research, biomedical. FISH, FRET, FRAP, FLIM, fluorescence, peltier cooled CCD sensors, ultra low light sensitivity, trace fluorescence. Knowledgeable staff.
The Nightseaâ ¢ fluorescence stereo microscope adapter equips most dissecting/stereo microscopes for use with fluorescence with no modification. - Page O15
www.MOLUNA.de Introduction to Fluorescence Sensing [4095772] - IntroductionnChapter 1. Basic principlesn1.1. Overview of strategies in molecular sensingn1.2. Labeled targets in fluorescence assaysn1.3. Competitor displacement assaysn1.4. Sandwich assaysn1.5. Catalytic biosensorsn1.6. Direct reagent-independent sensingnSensing and thinking 1: How to make the best sensor? Comparison of basic principlesnnChapter 2. Theoretical aspectsn2.1. Parameters that need to be optimized in every sensorn2.2.
Comprehensive whitepapers and specialist articles on the subject of fluorescence in biotechnology, life sciences, pharmacy, healthcare, diagnostics, bioinformatics. Find comprehensive information on the latest methods, processes, trends and applications.
52 mg of fluorescent product per mL of suspension, while in NC-RS100 and in NC-S100, the liquid portion was 333 μL/10 mL of suspension corresponding to learn more approximately 3.15 mg of fluorescent product per mL of suspension. It is important to note that … Continue reading →. ...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Discover our protocol describing the procedure of fluorescence activated cell sorting of live cell populations. Including tips to keep cells viable.
Be extraordinary. iStyles your HP TouchPad with a Fluorescence Blue HP TouchPad Skin. Vibrant, premium quality decal, no bulk, provides scratch protection.
The |i|Journal of Biomedical Optics|/i| (JBO) publishes peer-reviewed papers on the use of novel optical systems and techniques for improved health care and biomedical research.
Introduction to Fluorescence de David M. Jameson en Iberlibro.com - ISBN 10: 1439806047 - ISBN 13: 9781439806043 - CRC Press - 2014 - Tapa dura
When you express interest in a specific study, the information from your profile will be sent to the doctor conducting that study. If youre eligible to participate, you may be contacted by a nurse or study coordinator. If you select a health category rather than a specific study, doctors who have active studies in that area may contact you to ask if you would like to participate. In both cases, you will be contacted by the preferred method (email or phone) that you specified in your profile. ...
CLIP-Cell™ Block (bromothenylcytosine, BTC) is a non-fluorescent compound that blocks the reactivity of the CLIP-tag™ in vitro and inside or on the surface of living cells. It can be used to generate inactive controls in live cell labeling experiments performed with CLIP-tag fusion proteins. BTC reacts with CLIP-tag irreversibly, inactivating it for subsequent labeling steps.
Optical imaging is emerging as a powerful tool to study physiological, neurological, oncological, cell biological, molecular, developmental, immunological, and infectious processes
Objective Using real-time fluorescence quantitative PCR to detect mitochondrial DNA content changes within HepG2 cells induced by d4T and AZT. Methods HepG2 cells were treated with different concentrations(0,3,10,100,200,300μmol/L) of d4T and AZT respectively for two weeks. And then mitochondrial DNA contents were detected by real-time fluorescence quantitative PCR. Results Real-time fluorescence quantitative PCR was set up successfully to detect mitochondrial DNA contents. Mitochondrial DNA relative amounts were 96.94±5.77, 53.73±7.14, 20.78±3.10, 1.37±0.29 respectively with d4T concentrations of 0, 3, 10, 100μmol/L. The differences between groups were significant(P0.01). However, they were 96.94±5.77, 108.84±7.80, 172.56±4.70, 199.51±10.37, 158.74±6.64 and 64.06±6.27 respectively with AZT concentrations of 0, 3, 10, 100, 200, 300μmol/L, and the differences between groups were significant(P0.01). Conclusions It is practicable to detect mitochondrial DNA contents with real-time fluorescence
Red and green autofluorescence have been observed from dental plaque after excitation by blue light. It has been suggested that this red fluorescence is related to caries and the cariogenic potential of dental plaque. Recently, it was suggested that red fluorescence may be related to gingivitis. Little is known about green fluorescence from biofilms. Therefore, we assessed the dynamics of red and green fluorescence in real-time during biofilm formation. In addition, the fluorescence patterns of biofilm formed from saliva of eight different donors are described under simulated gingivitis and caries conditions. Biofilm formation was analysed for 12 hours under flow conditions in a microfluidic BioFlux flow system with high performance microscopy using a camera to allow live cell imaging. For fluorescence images dedicated excitation and emission filters were used. Both green and red fluorescence were linearly related with the total biomass of the biofilms. All biofilms displayed to some extent ...
Metabolic stress occurs at disease onset and causes altered flavoprotein redox activity that increases flavoprotein autofluorescence (FA).
Fluorescence describes the illumination created when a diamond is exposed to ultraviolet light. Faint of medium fluorescence rarely affects a diamonds appearance, but stronger fluorescence may have an impact on the perceived color. For instance, with a yellowish diamond with a strong blue fluorescence, the fluorescence can be strong enough to mask the yellowish tint when viewed under fluorescent lighting. When looked at under different lighting, that same diamond can look very different. Also, the opposite is possible as well - diamonds that fluoresce yellow may look more yellow under ultraviolet lighting, and whiter under incandescent lighting ...
Introduction: This is a preliminary report of an ongoing prospective bimodality lung cancer surveillance trial for high-risk patients. Bimodality surveillance incorporates autofluorescence bronchoscopy (AFB) and spiral CT (SCT) in high-risk patients as a primary lung cancer surveillance strategy, based entirely on risk factors. AFB was used for surveillance and findings were compared with conventional sputum cytology (CSC) for the detection of malignancy and premalignant central airway lesions. Eligibility: For eligibility, patients were required to have at least two of the following risk factors: 1) , 20 pack year history of tobacco use, 2) asbestos-related lung disease on chest radiograph, 3) COPD with an FEV-1 , 70% of predicted, and 4) prior aerodigestive cancer treated with curative intent, with no evidence of disease for , 2 years. All eligible patients under went AFB, a low dose SCT of the chest without contrast, and a sputum sample was collected for cytology. Bronchoscopy biopsy findings ...
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A new type of modulation fluorometer was used in the study of energy-dependent chlorophyll fluorescence quenching (qE) in intact leaves. Under conditions of strong energization of the thylakoid membrane (high light intensity, absence of CO2) not only variable fluorescence, FV, but also dark-level fluorescence, FO, was quenched, leading to definition of a quenching coefficient, qO. Information on qO was shown to be essential for correct determination of photochemical (qQ) and energy dependent quenching (qE) by the saturation pulse method. The relationship between qE and qO was analysed over a range of light intensities at steady state conditions. qE was found to consist of two components, the second of which is linearly correlated with qO. qO and the second component of qE are interpreted to reflect the state 1 - state 2 shift caused by LHC II phosphorylation.
Because fluorescence consists of photons at a longer wavelength than the excitation radiation, the fluorescence signal can be easily separated from the excitation light using a beamsplitter and/or filters. Unlike other forms of microscopy that utilise reflected or scattered light, this means that very weak fluorescence signals - down to a single photon - can be detected. In turn, this means that a single fluorescent tag can be imaged - enabling visualisation of the distribution of individual biological molecules. The figure shows the most common form of fluorescence microscope where excitation light is reflected by a dichroic beamsplitter and illuminates the field of view to produce fluorescence at a wavelength that is transmitted by the dichroic beamsplitter. The fluorescence image is formed by the action of the two lenses, known as the objective lens, which captures the light from the sample, and the tube lens that forms a real image at the camera. This configuration is called an ...
TY - JOUR. T1 - The sentinel margin. T2 - Intraoperative ex vivo specimen mapping using relative fluorescence intensity. AU - Van Keulen, Stan. AU - Nishio, Naoki. AU - Birkeland, Andrew. AU - Fakurnejad, Shayan. AU - Martin, Brock. AU - Forouzanfar, Tim. AU - Cunanan, Kristen. AU - Colevas, A. DImitrios. AU - Van Den Berg, Nynke S.. AU - Rosenthal, Eben. PY - 2019/8/1. Y1 - 2019/8/1. N2 - Purpose: Despite major advancements in surgical oncology, the positive margin rate for primary head and neck cancer resection remains around 15%-30%. In particular, the deep surface margin is the most challenging to adequately assess. Inadequate margins are directly correlated to poor survival, and as such, mitigation of these rates is critical to improve patient outcomes. We have developed an ex vivo imaging strategy that utilizes fluorescence intensity peaks (relative to background signal) of an injected anti-EGFR antibody conjugated to a fluorescent probe to locate potential close or positive margins on the ...
This image was produced using fluorescence microscopy, staining the cells with compounds that bind to the cell walls and fluoresce. The blue cells have walls made of cellulose and their blue fluorescence is due to the calcofluor that theyve been stained with, which fluoresces blue in ultraviolet light. Calcofluor has been used as a blue whitener in washing powders - it binds to the cellulose in cotton fabrics and fluoresces faintly blue in the UV component of sunlight. The yellow staining is due to another fluorescent dye (fluorochrome) called auramine O, which binds to cutin in the outer cuticle of the plant, and to dead, lignified cell walls that give the stem its strength - and it fluoresces yellow. The cuticle in this cross section is the thin yellow line covering the outer surface of the section. The yellow circle in the centre is composed of dead, lignified cells - not particularly well developed in goosegrass because it scrambles over surrounding vegetation rather then investing ...
D: The localization of Cy3-DNA fragments injected by yourself. Pink fluorescence: the Cy3-DNA fragments Blue fluorescence: the chromosomal DNAs. Transgene
Fast and non-invasive, diagnostic techniques based on fluorescence spectroscopy have the potential to link the biochemical and morphologic properties of tissues to individual patient care. One of the most widely explored applications of fluorescence spectroscopy is the detection of endoscopically invisible, early neoplastic growth in epithelial tissue sites. Currently, there are no effective diagnostic techniques for these early tissue transformations. If fluorescence spectroscopy can be applied successfully as a diagnostic technique in this clinical context, it may increase the potential for curative treatment, and thus, reduce complications and health care costs. Steady-state, fluorescence measurements from small tissue regions as well as relatively large tissue fields have been performed. To a much lesser extent, time-resolved, fluorescence measurements have also been explored for tissue characterization. Furthermore, sources of both intrinsic (endogenous fluorophores) and extrinsic ...
Frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) is a fast and accurate way of measuring fluorescence lifetimes in widefield microscopy. However, the resolution of multiple exponential fluorescence decays has remained beyond the reach of most practical FD-FLIM systems. In this paper we describe the implementation of FD-FLIM using a 40MHz pulse train derived from a supercontinuum source for excitation. The technique, which we term multi-harmonic FLIM (mhFLIM), makes it possible to accurately resolve biexponential decays of fluorophores without any a priori information. The systems performance is demonstrated using a mixture of spectrally similar dyes of known composition and also on a multiply-labeled biological sample. The results are compared to those obtained from time correlated single photon counting (TCSPC) microscopy and a good level of agreement is achieved. We also demonstrate the first practical application of an algorithm derived by G. Weber [1] for analysing mhFLIM ...
A biological fluorescence diagnostic apparatus having a device for irradiating a biological tissue with light which excites the tissue to generate fluorescent light, and a device for taking a fluorescence image of the biological tissue passing through an ocular optical system of an endoscope. The apparatus further has a television camera unit including a television camera for taking an ordinary endoscopic observation image passing through the ocular optical system, and a television camera with an image intensifier for taking a fluorescence observation image passing through the ocular optical system after amplifying the light intensity of the image. An optical path switching system which includes a reflective surface is selectively inserted and withdrawn from the optical path of light passing through the ocular optical system so as to selectively produce ordinary and fluorescence images in the television camera unit. A filter is selectively inserted into and movable out of an illuminating light path of
Background/Aims: In vivo autofluorescence endoscopic imaging and spectroscopy have been used to detect and differentiate benign ( hyperplastic) and preneoplastic ( adenomatous) colonic lesions. This fluorescence is composed of contributions from the epithelium, lamina propria, and submucosa. Because epithelial autofluorescence in normal and diseased tissues is poorly understood, this was the focus of the present study. Methods: Whole colonic crypts were isolated, and short term primary cultures of epithelial cells were established from biopsies of normal, hyperplastic, and adenomatous colon. Autofluorescence ( 488 nm excitation) was examined by confocal fluorescence microscopy. Fluorescently labelled organelle probes and transmission electron microscopy were used to identify subcellular sources of fluorescence. Results: Mitochondria and lysosomes were identified as the main intracellular fluorescent components in all cell types. Normal and hyperplastic epithelial cells were weakly ...
What is Fluorescence Spectroscopy? Fluorescence is a type of luminescence caused by photons exciting a molecule, raising it to an electronic excited state. Overview of what is fluorescence, what is a fluorescence spectrum, what materials exhibit fluorescence.
(a) Representative in vivo fluorescence images of MGC803-tumour-bearing mouse after iv-injected with FA-AlexaFluor647-labeled pRNA nanoparticle. The tumor areas