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Get an overview of FLUORESCITE (fluorescein sodium injection, solution), including warnings and precautions, directions, and the names of other drugs and products that include the same medication.
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Determination of β-glucan in carbohydrates was studied by detecting change of exciting wavelength and emission wavelength as sodium fluorescein was mixed with β-glucan, glucose, fructose and maltose. The results showed that if sodium fluorescein was mixed with β-glucan, UV-absorption of fluorescein sodium changed from 436.5 nm to 510.6 nm, fluorescence absorption also changed: exciting wavelength from 487.3 nm to 475.9 nm, emission wavelength from 517.6 nm to 510.6 nm, if sodium fluorescein was mixed with other carbohydrates such as glucose, fructose or maltose, no change happened for fluorescence absorption. The optimal proportion of sodium fluorescein and β-glucan was 6:1, the linear concentration range was 0.1 μg/mL~0.25 μg/mL, the regression equation: y = -2.014x + 1.075 7,R2 = 0.997 8, the purity of β-glucan in raw material was 95 % using such equation, comparing with 96 % by standard enzyme method.
Fluorescein injection, USP is a sterile solution for use intravenously as a diagnostic aid. It is a dark reddish orange solution with a pH of 8.3 to 9.8 and an osmolarity of 572 to 858mOsm/kg.. ...
PRIMARY OBJECTIVES:. I. To determine the feasibility of performing intravital microscopy on accessible human melanoma tumors during their standard course of treatment (i.e., wide local excision). (Part I). SECONDARY OBJECTIVES:. I. To determine the blood flow velocity of the tumor vessels and tissue penetration of fluorescein (fluorescein sodium) as a marker of tumor vessel permeability. (Part II). OUTLINE:. Patients receive fluorescein sodium intravenously (IV) followed by intravital microscopic observation over 10-15 minutes during excision of the melanoma.. After completion of study treatment, patients are followed up at 3 weeks and then periodically for 5 years. ...
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Anti-fluorescein antibodies are excellent model systems for studying the biochemical basis of molecular recognition because a prodigious amount of both physico-chemical and structural information is available for these antibodies. Furthermore, recombinant single-chain antibodies have been produced for several anti-fluorescein antibodies, and site-specific mutagenesis studies have defined the energetic contributions of a number of key active-site residues. In previous studies, we determined the three-dimensional structure of an antigen-binding fragment of a high-affinity anti-fluorescein antibody (4-4-20) in complex with fluorescein. These studies showed that fluorescein binds tightly in an aromatic slot and participates in a network of electrostatic interactions. In this report, we examine the role of electrostatic interactions in the 4-4-20 antigen-combining site by observing the effects of pH on the fluorescence of fluorescein and antigen-binding affinity. These studies showed that the salt ...
ANA M. MATA, ANN E. SCHOFIELD, JANE WOODBINE, ANTHONY G. LEE, J. MALCOLM EAST; Probing the nucleotide binding site of sarcoplasmic reticulum (Ca2+ -Mg2)-ATPase with anti-fluorescein antibodies. Biochem Soc Trans 1 December 1989; 17 (6): 1105-1106. doi: https://doi.org/10.1042/bst0171105. Download citation file:. ...
Sodium fluorescein leakage for mono-, co-, and tetraculture in transwell inserts with 0.4, 1 and 3 μm pore size. Medium containing 10 µg/mL of sodium fluores
Drug-coated balloons are medical devices designed to locally deliver drug to diseased segments of the vessel wall. For these dosage forms, drug transfer to the vessel wall needs to be examined in detail, since drug released into the blood is cleared from the site. In order to examine drug transfer, a new in vitro setup was developed combining the estimation of drug loss during advancement to the site of application in a model coronary artery pathway with a hydrogel compartment representing, as a very simplified model, the vessel wall. The transfer of fluorescent model substances as well as the drug paclitaxel from coated balloons to the simulated vessel wall was evaluated using this method. The model was suitable to quantify the fractions transferred to the hydrogel and also to qualitatively assess distribution patterns in the hydrogel film. In the case of fluorescein sodium, rhodamin b and paclitaxel, vast amounts of the coated substance were lost during the simulated passage and only very small
ALTAFLUOR (Flurox) Fluorescein Sodium and Benoxinate Hydrochloride Ophthalmic Solution, USP 0.25%/0.4% (Sterile) with Sterile Dropper NDC# 59390-218-05 5mL
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Nitric oxide ((center dot)NO) produced in plants is implicated in defense responses against pathogens. (center dot)NO synthesis in such conditions has been attributed to a nitric oxide synthase (NOS)-like enzyme and, more recently, to a mitochondrial-dependent NO(2)(-)-reducing activity. In this work, we used an NR-deficient double mutant (nia1 nia2) of Arabidopsis thaliana that is deficient in endogenous NO(2)(-) to analyse the hypersensitive response (HR) against an avirulent strain of Pseudomonas syringae pv. maculicola (Psm). The inoculation of Psm into nia1 nia2 A. thaliana caused leaf chlorosis whereas the HR was induced in wild-type plants. (center dot)NO production in situ was substantially increased in wild-type but not in nia1 nia2 leaves following inoculation of Psm, as measured with the fluorescent (center dot)NO indicator 4,5-diaminofluorescein diacetate. However, the infiltration of L-arginine or NO(2)(-) into nia1 nia2 leaves triggered (center dot)NO production in situ. Moreover, ...
Anti-Cat IgG (H&L) (Fluorescein Conjugated) Secondary Antibody, Rabbit Polyclonal, Fluorescein (FITC) validated in IF, FC (ASR1383), Abgent
Anti-Goat IgG F(ab)2 (Fluorescein Conjugated) Secondary Antibody, Rabbit Polyclonal, Fluorescein (FITC) validated in IF, FC (ASR3361), Abgent
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An article has been issued last month in the journal Theoretical Chemistry Accounts, discussing new insights into electrical bistability of organic films, in particular fluorescein dyes. The title of the paper is
While some ulcers can be seen with the naked eye or with magnification, one cannot tell whether the outermost layer of the corneal epithelium is damaged without staining it. It is this layer that is of unique conformation and "seals" the multiple-layer, onion-skin-like cornea from infiltration and dehydration.. It is important to know if the corneal epithelium is damaged because certain medications may lead to blindness in that circumstance. Its one more reason not to use leftover medication or other patients medications.. Even the slightest interruption of the outermost layer of the cornea will become evident when fluorescein stain is applied. Tiny ulcers might need illumination by ultraviolet or "black" light to become apparent.. Fluorescein stain is a sight-saving tool used by general practitioners and board-certified ophthalmologists alike.. See you tomorrow, Dr. Randolph.. MMFLUO. ...
Traditional fluorescein (FAM) fluorophore - universal dye useful for various applications, compatible with most of existing fluorescent instruments. All products are available as pure 5- or 6-isomers.
Subject: Re: fluorescein > >Storage should be in the dark, to prevent photo bleaching of FITC when >samples are collected, fixed, handled - etc. After cutting sections >must be in dark after mounting with antifade mounting media. Vector >has a nice one, Vectashield Hardset. > >She can probably do all three preparations, but remember that if she >fixes in formalin or paraformaldehyde, the tissue will have some >autofluorescence. If the FITC signal is stronger, brighter than then >dimmer autofluoresence, then all should go well. In general, the >microcapsules are huge! and very bright. > >I would think ingesting FITC might damage it (digestive enzymes, etc) >but not sure, there may be some pH and enzyme considerations. Have >her contact tech services at Molecular Probes, they usually have >answers for fluorochromes. > >We have used FITC labelled microcapsules injected into bovine tonsil, >removed tissue, snap freeze and cryosectioned and fixed sections >immediately with NBF. Some autofluorescence ...
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FITC is the fluorochrome of choice to prepare histological protein and antibody conjugates for use in fluorescence microscopy. Stable covalent bonds are obtained without destroying the fluorescent structure Unreacted material can easily be removed by dialysis or gel filtration. Fluorescence efficiency high and is only slightly effected on conjugation to proteins. It allows unambiguous histological and cytological interpretation because of good colour contrast with the tissue background, even offsetting reduced fluorescence under some conditions. FITC-antibody conjugates are stable under normal storage conditions, while immunological properties of antigens and antibodies are normally preserved. FITC-antibody conjugates are non-toxic and the fluorochrome does not add significantly to the antigenicity of the complex. Simple and rapid conjugation procedures which are relatively insensitive to experimental variables have been described ...
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Professional guide for Fluorescein Sodium/Proparacaine Hydrochloride. Includes: pharmacology, pharmacokinetics, contraindications, interactions, adverse reactions and more.
0249] Antioxidant is an agent that reduces the damage caused by oxidation. Antioxidant activity was determined by ORAC testing using an adaptation of the method described in "Performing Oxygen Radical Absorbance Capacity (ORAC) Assays with Synergy HT Multi-Detection Microplate Reader" Application Note from BioTek available at (www.biotek.com/resources/docs/ORAC_Assay_Application_Note.pdf) for use with Synergy 2 microplate reader from BioTek Instruments Inc (Winooski, Vt.). In this assay, AAPH (2,2-azobis 2-amino-propane) generates reactive oxygen species which damage the fluorescent probe (sodium fluorescein). Antioxidants such as (R)-Trolox methyl ether prevent or slow this damage, and their effects can be quantified by fluorescence measurements. Fluorescence readings were taken with excitation wavelength set at 485 nm and emission wavelength set at 528 nm, with reaction volume of 200 μA, AAPH concentration of 55 mM, sodium fluorescein concentration of 1.33 μM, and (R)-Trolox methyl ether ...
Define fluorescein. fluorescein synonyms, fluorescein pronunciation, fluorescein translation, English dictionary definition of fluorescein. n. An orange-red compound, C20H12O5, that exhibits intense fluorescence in alkaline solution and is used in medicine for diagnostic purposes, in...
Semantic Scholar extracted view of Specific Staining of Ornithosis Virus by Fluorescein-labelled Incomplete Antibodies. by Robert D. Goldin et al.
Since its introduction in the early 1960s, fluorescein angiography has proved to be an essential tool for diagnosis of many retinal disorders. This course will provide an overview of the use of fluorescein for diagnosing eye related diseases and conditions and include appropriate doses for adult and pediatric patients. Awareness of the side effects, adverse reactions, and complications of the contrast agent allow the ophthalmic healthcare team to anticipate, respond quickly, and support the patient before, during, and following the angiographic procedure using fluorescein ...
... is a test which allows the blood vessels in the back of the eye to be photographed as a fluorescent dye is
The Trans-Epithelial Permeability (TEP) Assay, is a cell-based assay used to evaluate the potential ocular irritancy of test chemicals by measuring the permeability of sodium fluorescein (or fluorescein leakage) through a confluent monolayer of Madin-Darby Canine Kidney (MDCK) cells. The MDCK cell line is used since it forms tight junctions in a confluent monolayer similar to those formed in the outermost corneal and conjunctival epithelial layers. The disruption of the tight junctions by chemicals is one of the early events typical... Trans-Epithial Permeability ...
Change from baseline in corneal staining with fluorescein at month 3. The cornea is the transparent front part of the eye which covers the iris and pupil. To detect the presence or absence of corneal puncta (tiny disruptions in the surface of the eye), fluorescein dye is administered into the eye and the eye is graded using a 5-point scale where 0 equals no puncta (best), and 3 equals too many puncta to count (worst). A negative number change from baseline indicates improvement ...
Your doctor may ask you not to eat the morning of your angiography. Your testing will most likely be performed in a specially equipped examination room. It does not require a surgery center or anesthesia. Prior to your procedure, your eye will be dilated.. During the test, a harmless orange-red dye called Fluorescein will be injected into a vein in your arm. The dye will travel through your body to the blood vessels in your retina. Your doctor will use a special camera with a green filter to flash a blue light into your eye and take multiple photographs. He will analyze the pictures and identify any damage to the lining of the retina or to spot the growth of new blood vessels.. This diagnostic test takes about 30 minutes to an hour, including the time for dilation of your eye. You can go home immediately after the procedure. After your angiography, your skin and urine may appear discolored for a short time until the Fluorescein is completely out of your system.. ...
A 30-year-old woman had a seizure. She had never had a seizure before. Understandably upset, her husband called 911 and the patient was brought to our emergency department.. By the time she arrived, she was still a little confused as is common after a seizure. I examined her and ordered tests to see if there was an identifiable cause of the seizure. When all of the tests came back normal, I went back to tell the patient and her husband the results.. I had noticed that, while we were talking, the patient kept her eyes closed and was tearful. I assumed this was from being emotional about this potentially life-changing event.. When I asked them if they had any questions, they wanted to know why her eyes hurt so badly. This explained why she had her tearful eyes closed but really puzzled me. I knew nothing about having a seizure that would cause one to have painful eyes.. When I looked at them more closely, they were very red and swollen. Staining with fluorescein dye showed both of her corneas (the ...
Uranine (sodium fluorescein, UR) has been routinely used in hydrological research to monitor surface and subsurface water flow, transport and mixing processes since the end of nineteenth century. Based on such obtained data, further conclusions can be drawn on the spread and behavior of pollutants (partly on models). Use of UR for qualitative (visual) studies of underground contamination is common, however data available on its environmental behavior (e.g., conversion, degradation or formation and fate of the transformation products, TPs) are incomplete or not readily comparable. UR observations of biodegradation are still speculative. S-metolachlor (SM) is a popular worldwide chloroacetamide herbicide, which highly correspond to the global pesticide use. It is offered on the French market as an effective multicrop herbicide against annual grasses and certain broadleaf weeds under the trade name Mercantor Gold (MG). Photodegradation contributes to the fate of SM in the aquatic environment. TPs ...
Electroporation affects the transfer of fluorescein through inner membranes, but not the chorion. Embryos were incubated with 10 μg/ml fluorescein for 40 minut
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Fluorescein is a fluorescent dye thats used in everything from eye exams to oceanography. Many researchers use fluorescein to...
The retina is complex and is composed of nerve layers and blood vessels as well as other tissues. The blood vessels that are located in the retina are able to be examined using a special technique and camera called Intravenous Fluorescein Angiography (IVFA). In this procedure, the eye is dilated, and a special dye is injected into the arm. Within 10-15 seconds, the dye that was injected into the arm passes through the blood vessels located in the back of the eye. When the progression of dye occurs, photographs are taken. The filling of the blood vessels with as well as the clearing of the dye allows the physician to see what parts of the eye are healthy or unhealthy. This information allows the physician to target treatment to the most affected part of the eye ...
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Fluorescein Angiography Testing uses a special camera to view both the front and the back of the retina. Schedule an exam today at our Boca Raton or Coral Springs offices.
5(6)-FITC (Fluorescein 5(6)-isothiocyanate), Fluorescent amine-reactive label (CAS 27072-45-3), with |90% purity. Join researchers using our high quality biochemicals.
Page contains details about fluorescein isothiocyanate-loaded targeted lipid-polymer hybrid nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Fluorescein is used in medicine to diagnose corneal ulcers and certain other eye disorders. A solution of the chemical is dripped into the eye, and a ty...
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FLUORESCEIN CHLORIDE 630-88-6 NMR spectrum, FLUORESCEIN CHLORIDE H-NMR spectral analysis, FLUORESCEIN CHLORIDE C-NMR spectral analysis ect.
Fluorescein dye is available in several formulations, including a small paper strip that can be placed directly onto the eye and a liquid solution that can be applied into the eye.. Corneal injuries may be invisible, but they tend to be very painful. Animals with these injuries may have red, swollen, watery eyes, or may squint or rub their eyes. If your veterinarian suspects your pet may have an injury on the cornea, a small amount of fluorescein dye is applied to the surface of the cornea. If the corneal surface is intact, the fluorescein dye will not stick to the eye. However, if there is a scratch, ulcer, or defect on the cornea, the dye sticks to the injured area and can show your veterinarian where and how serious the injury is.. Performing a fluorescein stain takes only a few minutes and is not painful for your pet.. What Does a Fluorescein Stain Tell Your Veterinarian?. Fluorescein staining can tell your veterinarian if your pet has a scratch, ulcer, or other defect on the surface of the ...