TY - JOUR. T1 - Effect of basic fibroblast growth factor on angiogenesis in the infarcted porcine heart. AU - Watanabe, E.. AU - Smith, D. M.. AU - Sun, J.. AU - Smart, F. W.. AU - Delcarpio, J. B.. AU - Roberts, T. B.. AU - Van Meter, C. H.. AU - Claycomb, W. C.. PY - 1998/2/1. Y1 - 1998/2/1. N2 - Administration of growth factors is emerging as a new therapeutic approach for the enhancement of collateral vessel formation in the ischemic heart. We have investigated the effects of intramyocardial delivery of FGF-2 in the presence and absence of heparin on angiogenesis in a porcine model of myocardial infarction. Yorkshire pigs were subjected to myocardial infarction by the placement of an embolization coil in the left anterior descending artery (n = 5). Four to five weeks after creation of an infarct, FGF-2 (10 μg) alone or in complex with heparin, heparan sulfate, or heparin agarose beads was injected either into the normal myocardium or along the infarct border area. Histologic evaluation of ...

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The effect of basic fibroblast growth factor (b-FGF), one of the commonest angiogenic factors in various cancer types, on lymphocyte adhesion and transmigration across the endothelial cell monolayer was investigated using human umbilical vein-derived endothelial cells (HUVEC) and type I collagen gel. Forty-eight h exposure of HUVEC with 2 ng/ml b-FGF significantly decreased the basal adhesion of lymphocytes to endothelial cells. The decrease ratio is further enhanced by the addition of shear stress in this assay system. When HUVEC was stimulated for the last 24 h with optimal conditions of recombinant interleukin 1β, the percentages of transmigration as well as adhesion were also decreased significantly by the presence of b-FGF. The expression of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 was down-regulated by b-FGF exposure in both resting and activated conditions by recombinant interleukin 1β, supposedly the main reason for this phenomenon. The migrating cells ...

The aim here was to explore a new graft material that excludes the need to harvest autogenous bone from patients. Forty-two critical-size (10 x 15 mm) defects were created in rabbit mandibles bilaterally. Five groups of six defects each were grafted with autogenous endochondral (EC) bone, autogenous intramembranous (IM) bone, fresh-frozen allogeneic IM bone only, fresh-frozen allogeneic IM bone and demineralized bone matrix powder prepared from intramembranous bone (DBM IM) only, and fresh-frozen allogeneic IM bone and basic fibroblast growth factor (bFGF) mixed with DBM IM powder. The remaining defects were used as controls. Three weeks after surgery, the defects were retrieved for histological analysis. The amount of new bone formation was quantified by image analysis. No bone formed across the defect in the controls; 224% more new bone formed in defects grafted with composite allogeneic IM bone/DBM IM than in those grafted with allogeneic IM bone alone (p < 0.001); 550% more new bone was ...

Migration of endothelial cells is involved in normal and pathological angiogenesis and in re-endothelialization after vascular injury or rupture of atherosclerotic plaques. Several types of endothelial cells are known to synthesize basic fibroblast growth factor (bFGF); in some of these, migration is increased by exogenous bFGF and inhibited by anti-bFGF antibodies. Using immunocytochemical techniques and RNase protection analysis, we studied endothelial cells from bovine coronary arteries and veins as well as from adrenal microvessels. We found that bFGF mRNA and peptide were present in confluent endothelial cells and were upregulated during migration stimulated by removal of some cells from the monolayer. During migration, extracellular matrix stores of bFGF were depleted, and bFGF immunoreactivity began to accumulate in the cytoplasm of endothelial cells between 2 and 6 hours. After migration had begun, but before the initiation of DNA synthesis, bFGF immunoreactivity increased in the nuclei ...

TY - JOUR. T1 - O6-Methylguanine-DNA-methyltransferase promoter demethylation is involved in basic fibroblast growth factor-induced resistance against temozolomide in human melanoma cells. AU - Fontijn, Dennis. AU - Adema, Auke D.. AU - Bhakat, Kishor K.. AU - Pinedo, Herbert M.. AU - Peters, Godefridus J.. AU - Boven, Epie. PY - 2007/10/1. Y1 - 2007/10/1. N2 - Basic fibroblast growth factor (bFGF) is a multifunctional protein and one of the most important growth factors in cutaneous melanoma development and progression. We hypothesized that high bFGF expression might be responsible for chemoresistance in advanced melanoma. M14 human melanoma cells expressing low levels of bFGF were successfully transfected with vectors encoding either the 18 kDa or all isoform proteins of bFGF. M14 cells and bFGF-overexpressing clones had a similar growth rate in vitro. Overexpression of 18 kDa or all isoform proteins of bFGF resulted in, respectively, 2.9- and 6.9-fold resistance against temozolomide. ...

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Basic fibroblast growth factor (bFGF) is a trophic factor synthesized in the central nervous system (CNS), where it is believed to play a role in neuronal maintenance and repair. Little is known about the regulation of this growth factor in the CNS. To determine whether the expression of the bFGF gene in the brain of adult animals changes in response to alterations of neuronal activity, we examined bFGF mRNA levels in several brain regions of rats experiencing focally-evoked convulsive seizures. Seizures were induced by microinjecting bicuculline unilaterally into an epileptogenic site within the deep prepiriform cortex, area tempestas (AT). By 5 h after initiation of brief limbic motor seizures from AT, there was a four fold increase in the levels of bFGF mRNA in the entorhinal cortex, hippocampus and olfactory bulb, but not in the caudate-putamen. The maximal expression of bFGF mRNA was reached by 10 h after seizure onset. In the same animals, the mRNA encoding nerve growth factor (NGF) was ...

TY - JOUR. T1 - Ultrastructural immunolocalization of basic fibroblast growth factor in mast cell secretory granules. T2 - Morphological evidence for bFGF release through degranulation. AU - Qu, Zhenhong. AU - Kayton, Robert J.. AU - Ahmadi, Proochista. AU - Liebler, Janice M.. AU - Powers, Michael R.. AU - Planck, Stephen R.. AU - Rosenbaum, James T.. PY - 1998/10. Y1 - 1998/10. N2 - We previously reported that mast cells (MCs) serve as a source of basic fibroblast growth factor (bFGF), a potent angiogenic and mitogenic polypeptide, suggesting that bFGF may mediate MC-related neovascularization and fibroproliferation. Unlike many other growth factors, bFGF lacks a classic peptide sequence for its secretion, and the mechanism(s) for its release remains controversial. Because MCs release a wide spectrum of bioactive products via degranulation, we hypothesized that MC degranulation may be a mechanism of bFGF release and used ultrastructural immunohistochemistry to test the hypothesis. We reasoned ...

1FGA: Refinement of the structure of human basic fibroblast growth factor at 1.6 A resolution and analysis of presumed heparin binding sites by selenate substitution.

We used trafermin (recombinant human basic fibroblast growth factor) to treat a skin ulcer (diameter, 1 cm; depth, 2 mm) that… Expand ...

Basic fibroblast growth factor, also known as bFGF, FGF2 or FGF-β, is a member of the fibroblast growth factor family. In normal tissue, basic fibroblast growth factor is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide. It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels, a process known as angiogenesis. In addition, it is synthesized and secreted by human adipocytes and the concentration of bFGF correlates with the BMI in blood samples. In this study, bFGF was also shown to act on preosteoblasts - in the form of an increased proliferation - after binding to fibroblast growth factor receptor 1 and activating phosphoinositide 3-kinase. bFGF has been shown in preliminary animal studies to protect the heart from injury associated ...

Basic fibroblast growth factor (bFGF) and acidic fibroblast growth factor (aFGF) are involved in the induction of embryonic mesoderm, angiogenesis, neuronal differentiation, and proliferation and survival of many cell types. In cardiac myocytes their roles are not well understood. Effects of fibroblast growth factors on reexpression of fetal actin genes have been reported. In freshly isolated adult rat cardiac myocytes, bFGF mRNA was not detectable by in situ hybridization, although the cells contained significant amounts of bFGF and aFGF as quantified by radioimmunoassays, mitogen assays with immunoneutralization, and Western blotting. After culturing, bFGF mRNA was detected (aFGF mRNA was not studied), and the cells contained 2.5-fold more bFGF and 60% more aFGF than freshly isolated cells. The FGFs were not found in conditioned medium. They were localized, especially in cultured cells, to the nucleus. Cultured myocytes bound fourfold more 125I-FGF than freshly isolated cells and expressed the ...

TY - JOUR. T1 - Induction of chondrogenesis. T2 - Requirement for synergistic interaction of basic fibroblast growth factor and transforming growth factor-beta. AU - Frenz, Dorothy A.. AU - Liu, Wei. AU - Williams, James D.. AU - Hatcher, Victor Bernard. AU - Galinovic-Schwartz, Vera. AU - Flanders, Kathleen C.. AU - Van De Water, Thomas R.. PY - 1994/2. Y1 - 1994/2. N2 - Interactions between the epithelial anlage of the developing mouse inner ear and its associated periotic mesenchyme control the differentiation of the cartilaginous otic capsule. Transforming growth factor-beta1 (TGF-β1) is a naturally occurring signal peptide that is present in these tissues at times of active differentiation and morphogenesis. Previous studies have shown that TGF-β1 alone is not a sufficient stimulus to initiate chondrogenesis in cultured periotic mesenchyme. In this study, we provide evidence that basic fibroblast growth factor (bFGF) can elicit a specific but limited chondrogenic response in cultured ...

TY - JOUR. T1 - Basic fibroblast growth factor and ultraviolet B transform melanocytes in human skin. AU - Berking, Carola. AU - Takemoto, Richelle. AU - Satyamoorthy, Kapaettu. AU - Elenitsas, Rosalie. AU - Herlyn, Meenhard. PY - 2001. Y1 - 2001. N2 - Ultraviolet (UV) light is an epidemiological risk factor for melanoma, but its specific contribution to melanoma induction is not known. The first critical step of melanoma development, ie, the uncontrolled proliferation of melanocytes, may be induced by a combination of UV damage and an imbalance of growth factor production by cells in the immediate area of the melanocyte. Among several candidates, basic fibroblast growth factor (bFGF) is the major autocrine growth factor in melanoma and associated with tumor progression. Overexpression of bFGF via adenoviral gene transfer in human skin xenografted to severe combined immunodeficiency mice led to black-pigmented macules within 3 weeks of treatment. Immunofluorescence analysis demonstrated ...

TY - JOUR. T1 - Generation of spinal motor neurons from human fetal brain-derived neural stem cells. T2 - Role of basic fibroblast growth factor. AU - Jordan, Paivi M.. AU - Ojeda, Luis D.. AU - Thonhoff, Jason R.. AU - Gao, Junling. AU - Boehning, Darren. AU - Yu, Yongjia. AU - Wu, Ping. PY - 2009/2/1. Y1 - 2009/2/1. N2 - Neural stem cells (NSCs) have some specified properties but are generally uncommitted and so can change their fate after exposure to environmental cues. It is unclear to what extent this NSC plasticity can be modulated by extrinsic cues and what are the molecular mechanisms underlying neuronal fate determination. Basic fibroblast growth factor (bFGF) is a well-known mitogen for proliferating NSCs. However, its role in guiding stem cells for neuronal subtype specification is undefined. Here we report that in-vitro-expanded human fetal forebrain-derived NSCs can generate cholinergic neurons with spinal motor neuron properties when treated with bFGF within a specific time window. ...

Basic fibroblast growth factor (bFGF) has been implicated in the brains trophic response to injury. This thesis examined the effects of endogenous bFGF on brain plasticity and recovery of behavioral function following cortical injury in adult rats. The first experiment investigated the post-lesion time course of the astrocytic expression of bFGF. Subsequent experiments examined the effects of injury-induced bFGF on neuroonal morphology, cortical morphology, and post-lesion behavioral deficits. Following motor cortex injury, endogenous bFGF prevented neuritic degeneration in layer V pyramidal neurons in Zilles area Fr2 and promoted recovery of function in the Whishaw Reaching Task. Housing rats in an enriched environment prior to cortical injury enhanced the expression of bFGF but did not increase cortical thickness nor reduce post-lesion behavioral deficits (relative to laboratroy-housed rats). Collectively, these experiments indicate that injury-induced bFGF plays a role in potentiating ...

Recently identified BLast Colony Forming Cells (BL-CFCs) from in vitro differentiated embryonic stem (ES) cells represent the common progenitor of hematopoietic and endothelial cells, the hemangioblast. Access to this initial cell population committed to the hematopoietic lineage provides a unique opportunity to characterize hematopoietic commitment events. Here, we show that BL-CFC expresses the receptor tyrosine kinase, Flk1, and thus we took advantage of the BL-CFC assay, as well as fluorescent activated cell sorter (FACS) analysis for Flk1(+) cells to determine quantitatively if mesoderm-inducing factors promote hematopoietic lineage development. Moreover, we have analyzed ES lines carrying targeted mutations for fibroblast growth factor receptor-1 (fgfr1), a receptor for basic fibroblast growth factor (bFGF), as well as scl, a transcription factor, for their potential to generate BL-CFCs and Flk1(+) cells, to further define events leading to hemangioblast development. Our data suggest that ...

The tissue sections were deparaffinized and rehydrated, and endogenous peroxidase activity was quenched with 0.6% H2O2 in methanol. A mouse monoclonal antibody (148.6.1.1, a kind gift from Dr C. Hart, ZymoGenetics, Seattle, Wash) was used for detection of bFGF. This antibody was raised against human recombinant bFGF, and its specificity was determined by Western blot analysis and with an affinity support column containing immobilized human recombinant bFGF.17 A previously described method served for detection of bFGF in MCs.11 Briefly, the sections were treated with hyaluronidase for antigen retrieval. To exclude nonspecific binding, the sections were incubated in a blocking solution containing 3% normal horse serum, 0.5% BSA, and 0.3% Triton X-100 in PBS. They were then incubated overnight with the anti-bFGF antibody (0.05 μg/mL) diluted in PBS containing 2% normal horse serum. The avidin-biotin complex method (ABC-AP Kit, Vector Laboratories) was used to detect the primary antibody with ...

High-affinity binding of basic fibroblast growth factor (bFGF) to the tyrosine kinase receptor requires cell-surface heparan sulfate proteoglycan or exogenous addition of heparin. The crystal structure of bFGF shows Arg40 and 45 on the surface opposite to the heparin-binding region, suggesting that these charged residues may be involved in the receptor binding. Therefore, these amino acids were mutated to aspartic acid separately or simultaneously, and also a simultaneous mutation to glutamic acid was introduced. These mutants displayed a mitogenic activity decreased greater than tenfold compared to the wild-type protein. Addition of heparin had no effect on the activity, while these mutants showed heparin-binding characteristics resembling those of the native sequence protein. The mutants exhibited decreased stability compared to the native sequence protein. Gradual changes in conformation were observed by circular dichroic and infrared spectroscopy. Heparin chromatography also showed the ...

The presence of basic fibroblast growth factor (bFGF) was investigated in neuronal cells derived from 12 and 18 week-old human fetal brain cultures. To this purpose, the ability of bFGF to stimulate plasminogen activator (PA) production in fetal bovine aortic endothelial GM 7373 cells was used as an assay for this molecule in neuronal cell extracts. The identity of the PA-stimulating activity of neuronal cell extract with bFGF was confirmed by its high affinity for heparin and by its cross-reactivity with polyclonal antibodies to human placental bFGF. These antibodies recognized a Mr 18,000 cell-associated protein both in Western blot and in immuno-precipitation experiments. All the neurons showed bFGF immunoreactivity, as demonstrated by immunocytochemical staining, while nonneuronal cells were unstained. The data demonstrate for the first time that cultured human fetal brain neurons contain and synthesize bFGF.

CELLUGENA FIBROBLAST GROWTH FACTORS Fibroblast Growth factors are naturally occurring specialized proteins capable of stimulating cellular growth, proliferation, healing, and cellular differentiation. Growth factors play a pivotal role in cellular process regulation, by acting as signaling molecules between cells to pr

Although basic fibroblast growth factor (bFGF) is a classical mitogen and survival factor in fibroblasts and endothelial cells, it inhibits proliferation in breast cancer cells. We investigated the survival effects of bFGF in MCF-7 breast cancer cells to determine if this effect was also paradoxic. Our data confirmed that bFGF increased clonogenic survival of NIH 3T3 fibroblasts alone and prior to treatment with etoposide or 5-fluorouracil, two chemotherapeutic agents with different mechanisms of action, but decreased clonogenic survival of MCF-7 cells and increased their susceptibility to these chemotherapeutic agents in a dose and time dependent manner. Similarly, bFGF preincubation increased programmed cell death or apoptosis in these cells and was additive with the apoptotic effects of etoposide and 5-FU as determined by morphologic criteria and by DNA fragmentation assayed by 3-OH dUTP-FITC end labeling. These effects correlated with bFGF-induced decreases in Bcl-2 mRNA and protein levels and a

The earliest characterized events during induction of tubulogenesis in renal anlage include the condensation or compaction of metanephrogenic mesenchyme with the concurrent upregulation of WT1, the gene encoding the Wilms tumor transcriptional activator/suppressor. We report that basic fibroblast growth factor (FGF2) can mimic the early effects of an inductor tissue by promoting the condensation of mesenchyme and inhibiting the tissue degeneration associated with the absence of an inductor tissue. By in situ hybridization, FGF2 was also found to mediate the transcriptional activation of WT1 and of the hepatocyte growth factor receptor gene, c-met. Although FGF2 can induce these early events of renal tubulogenesis, it cannot promote the epithelial conversion associated with tubule formation in metanephrogenic mesenchyme. For this, an undefined factor(s) from pituitary extract in combination with FGF2 can cause tubule formation in uninduced mesenchyme. These findings support the concept that ...

TY - JOUR. T1 - Mutants of basic fibroblast growth factor identity different cellular response programs. AU - Leenders, W.P.J.. AU - van Hinsbergh, V.W.M.. AU - van Genesen, S.T.. AU - Schoenmakers, J.G.G.. AU - Zoelen, E.J.J.. PY - 1997. Y1 - 1997. U2 - 10.3109/08977199709021521. DO - 10.3109/08977199709021521. M3 - Article. VL - 14. SP - 213. EP - 228. JO - Growth Factors. JF - Growth Factors. SN - 0897-7194. ER - ...

PD166866 (N-[2-Amino-6-(3,5-dimethoxyphenyl)pyrido[2,3-d]pyrimidin-7-yl]-N-(1,1-dimethylethyl)-urea) -Inhibitor of basic Fibroblast Growth Factor (bFGF)

TY - JOUR. T1 - Basic fibroblast growth factor supports expansion of mouse compact bone-derived mesenchymal stem cells (MSCs) and regeneration of bone from MSC in vivo. AU - Yamachika, Eiki. AU - Tsujigiwa, Hidetsugu. AU - Matsubara, Masakazu. AU - Hirata, Yasuhisa. AU - Kita, Kenichiro. AU - Takabatake, Kiyofumi. AU - Mizukawa, Nobuyoshi. AU - Kaneda, Yoshihiro. AU - Nagatsuka, Hitoshi. AU - Iida, Seiji. PY - 2012/4. Y1 - 2012/4. N2 - Some progress has been made in development of methods to regenerate bone from cultured cells, however no method is put to practical use. Here, we developed methods to isolate, purify, and expand mesenchymal stem cells (MSCs) from mouse compact bone that may be used to regenerate boneinvivo. These cells were maintainedinlong-term culture and were capable of differentiating along multiple lineages, including chondrocyte, osteocyte, and adipocyte trajectories. We used standard cell isolation and culture methods to establish cell cultures from mouse compact bone and ...

Treatment of basic fibroblast growth factor (bFGF) with organic disulfides, preferably glutathione disulfide, or with inorganic compounds of similar function results in a bFGF composition of enhanced stability and resistance to multimerization. The resulting stabilized form mimics the chromatographic behavior of bFGF as isolated from bovine pituitary.

We examined the localization of basic fibroblast growth factor (bFGF) in the developing embryonic and newborn rat nervous system using 2 anti-bFGF antibodies. Embryonic (E13, E14, E15, E16, E17, and E18) and newborn tissues were examined. Between E16 and E17 strong bFGF immunoreactivity (IR) was det...

The cardiovascular effects of proteolysis of high molecular weight basic fibroblast growth factor by inflammatory serine proteases

Systemic basic fibroblast growth factor induces favorable histological changes in the corpus cavernosum of hypercholesterolemic rabbits.

We set out to determine whether advanced epithelial ovarian cancer (EOC) is associated with elevated serum and ascitic concentrations of the angiogenic factors angiogenin (ANG), basic fibroblastic growth factor (bFGF), and vascular endothelial growth factor (VEGF), and whether the expression of angiogenic factors was associated with tumor vascularity. Serum and ascitic samples were collected from previously untreated patients with FIGO stage III and IV EOC and stored at -70 degreesC. Levels of the three factors were determined by enzyme-linked immunoassay. Histological sections from paraffin blocks of ovarian cancers were stained immunochemically for factor VIII, CD34, and VEGF. Thirty-nine patients were studied, although not all had paired serum and ascitic samples. For each angiogenic factor, the following findings were noted: (a) there was a wide range in serum and ascitic fluid concentrations; (b) the mean serum concentration was higher (P , 0.05) than the mean concentration in normal serum; ...

TY - THES. T1 - Melanoma, the role of basic Fibroblast Growth Factor in the aggressive behaviour and chemotherapy response. AU - Fontijn, D.. N1 - Naam instelling promotie: S.l.. PY - 2009. Y1 - 2009. M3 - Research VU University Amsterdam, graduation VU University Amsterdam. SN - 9789086593200. PB - s.n.. CY - S.l.. ER - ...

Metabolic effects of basic fibroblast growth factor in streptozotocin-induced diabetic rats: A 1H NMR-based metabolomics investigation. Related

Basic Fibroblast Growth Factor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 366 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

Li Liu, Xin-Zi Yu, Tie-Shi Li, Lian-Xia Song, Pei-La Chen, Ta-Lin Suo, Ying-Hua Li, Shi-Dong Wang, Yue Chen, Yong-Ming Ren, Shu-Ping Zhang, Zhi-Jie Chang, Xin-Yuan Fu*. A Novel Protein Tyrosine Kinase NOK that Shares Homology with Platelet-Derived Growth Factor/Fibroblast Growth Factor Receptors Induces Tumorigenesis and Metastasis in Nude Mice. Cancer Research. 64, 3491-3499, 2004. ...

TY - JOUR. T1 - DNA sequence and nucleosome placement on the murine fibroblast growth factor-4 gene. AU - Wilder, Phillip J.. AU - Mountjoy, Charles. AU - MacLeod, Michael C.. AU - Rizzino, Angie. PY - 1997. Y1 - 1997. N2 - A large portion of the 5′ flanking region of the fibroblast growth factor-4 (PGF-4) gene has been sequenced, but only 38 bp of sequence information past the end of the last exon of this gene has been described. In this study, a total of 2769 bp of nucleotide sequence downstream of the last exon of FGF-4 (GenBank #U43515) was determined by a combination of deletional subcloning and primer walking. In addition, we have used the theoretical algorithm of Calladine and Drew to predict the rotational positioning of nucleosomes throughout the entire FGF-4 gene.. AB - A large portion of the 5′ flanking region of the fibroblast growth factor-4 (PGF-4) gene has been sequenced, but only 38 bp of sequence information past the end of the last exon of this gene has been described. In ...

https://doi.org/10.18632/oncotarget.1312 Xiaoping Wu, Huixian Huang, Cong Wang, Shaoqiang Lin, Yadong Huang, Yi Wang, Guang Liang, Qiuxia Yan, Jian Xiao, Jianzhang Wu, Yongguang Yang, Xiaokun Li

TY - JOUR. T1 - Fibroblast Growth Factor-2 Antagonist Activity and Angiostatic Capacity of Sulfated Escherichia coli K5 Polysaccharide Derivatives. AU - Leali, Daria. AU - Belleri, Mirella. AU - Urbinati, Chiara. AU - Coltrini, Daniela. AU - Oreste, Pasqua. AU - Zoppetti, Giorgio. AU - Ribatti, Domenico. AU - Rusnati, Marco. AU - Presta, Marco. PY - 2001/10/12. Y1 - 2001/10/12. N2 - The angiogenic basic fibroblast growth factor (FGF2) interacts with tyrosine kinase receptors (FGFRs) and heparan sulfate proteoglycans (HSPGs) in endothelial cells. Here, we report the FGF2 antagonist and antiangiogenic activity of novel sulfated derivatives of the Escherichia coli K5 polysaccharide. K5 polysaccharide was chemically sulfated in N- and/or O-position after N-deacetylation. O-Sulfated and N,O-sulfated K5 derivatives with a low degree and a high degree of sulfation compete with heparin for binding to 125I-FGF2 with different potency. Accordingly, they abrogate the formation of the HSPG-FGF2-FGFR ternary ...

The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...

Shop Fibroblast growth factor ELISA Kit, Recombinant Protein and Fibroblast growth factor Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.

Recombinant Fibroblast Growth Factor 13 (FGF13) Protein. Species: Human. Source: Escherichia coli (E. coli). Order product ABIN6304346.

Kaplan-Meier progression-free survival (A) and overall survival (B) curves according to bFGF expression for 39 patients with NHL. bFGF expression was positive

TY - JOUR. T1 - Gene targeted ablation of high molecular weight fibroblast growth factor-2. AU - Azhar, Mohamad. AU - Yin, Moying. AU - Zhou, Ming. AU - Li, Hongqi. AU - Mustafa, Marwan. AU - Nusayr, Eyad. AU - Keenan, Jack B.. AU - Chen, Hwudaurw. AU - Pawlosky, Sharon. AU - Gard, Connie. AU - Grisham, Christina. AU - Sanford, L. Philip. AU - Doetschman, Tom. PY - 2009/2/1. Y1 - 2009/2/1. N2 - Fibroblast growth factor-2 (FGF2) is produced as high molecular weight isoforms (HMW) and a low molecular weight isoform (LMW) by means of alternative usage of translation start sites in a single Fgf2 mRNA. Although the physiological function of FGF2 and FGF2 LMW has been investigated in myocardial capillarogenesis during normal cardiac growth, the role of FGF2 HMW has not been determined. Here, we report the generation of FGF2 HMW-deficient mice in which FGF2 HMW isoforms are ablated by the Tag-and-Exchange gene targeting technique. These mice are normal and fertile with normal fecundity, and have a ...

Fibroblast growth factor 6 (FGF-6) is a heparin-binding growth factor that is expressed in epithelial and mesenchymal lineages. FGF-6 binds and signals through the FGF receptors FGFR1, FGFR2, and FGFR4. FGF-6 functions as a mitogen for vascular endothelial cells and fibroblasts. FGF-6 is also an important factor driving muscle differentiation and regeneration. This product is produced using Animal Free raw components and processes, in a non-mammalian system. Ships ambient. Store frozen at -20 to -10C. ...

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There is an increased incidence of heart failure in individuals with diabetes mellitus (DM). The co-existence of kidney disease in DM exacerbates the cardiovascular prognosis. Researchers have attempted to combine the critical features of heart failure, using transverse aortic constriction, with DM in mice but variable findings have been reported. Furthermore, kidney outcomes have not been assessed in this setting thus its utility as a model of heart failure in DM and kidney disease is unknown. We generated a mouse model of obesity, hyperglycemia and mild kidney pathology by feeding male C57BL/6J mice a high fat diet (HFD ...

MAA551Hu22, Monoclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2), 碱性成纤维细胞生长因子(FGF2)单克隆抗体, B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2 | 仅供体外研究使用，不用于临床诊断！请索取进口关税税单及报关单！

Isolation and in vitro expansion of progenitor cells from breast tumor specimens. Tumor specimens were obtained from consenting patients according to the Internal Review and the Ethics Boards of the Istituto Nazionale Tumori of Milan, Italy. Sixteen breast lesions, from the histologic diagnostic assessment and sampled by pathologists, were received in the Laboratory within 30 minutes of surgery and immediately mechanically disaggregated. Occasionally, enzymatic digestion was also required and tissue fragments were incubated at 37°C for 2 hours in a 1:1 solution of collagenase/hyaluronidase (Roche Diagnostics GmbH, Mannheim, Germany and Sigma-Aldrich Corp., St. Louis, MO, respectively). After filtration through a 30 μm pore filter, single cells were plated at 1,000 cells/mL in serum-free DMEM-F12 (Cambrex BioScience, Venviers, Belgium), supplemented with 10 ng/mL basic fibroblast growth factor (bFGF), 20 ng/mL epidermal growth factor (EGF), 5 μg/mL insulin, and 0.4% bovine serum albumin (all ...

also reported a similar profile of gene expression: the expression of CD90 as 18.4% in CDCs, compared with an expression of 99.0% in BM-MSCs (22). Thy-1 knock-out mice are viable and grossly normal (31), and the definite roles of CD90− clones in CVR are not clear, but some researchers reported data regarding this topic. They demonstrated the superiority of CD90− CDCs over CD90＋ CDCs (9, 11, 12). In the chronic MI rat model, injection of CD90− CMSCs augmented cardiac function outperforming CD90＋ cells, and histological analysis of CD90− cells revealed an increase in vascularization within the infarct lesion (12). Other studies reported similar salutary effects, since CD90− cells secret less inflammatory cytokines and may differentiate into cardiomyocytes (9). Interestingly, CD90− cells expressed significantly more hepatocyte growth factor (HGF) than did CD90＋ cells, and both CD90− and CD90＋ CMSCs secrete VEGF or basic fibroblast growth factor (bFGF) comparably (12).. By ...