Residualizing labels are tracers which remain in lysosomes after uptake and catabolism of the carrier protein and have been especially useful for studies on the sites of plasma protein degradation. Thus far these labels have contained radioactive reporters such as 3H or 125I. In the present paper we describe a fluorescent residualizing label, NN-dilactitol-N′-fluoresceinylethylenediamine (DLF). Modification of asialofetuin (ASF) or rat serum albumin (RSA) with DLF affected neither their normal kinetics of clearance from the rat circulation nor their normal tissue sites of uptake and degradation. After injection of DLF-ASF, fluorescent degradation products were recovered nearly quantitatively in liver and retained with a half-life of about 2 days. Fluorescent degradation products from DLF-RSA were recovered in skin and muscle, and were localized in fibroblasts by fluorescence microscopy. These results confirm previous studies with radioactive residualizing labels in which fibroblasts in ...

The endocytosis of 125I-labeled asialofetuin by rat hepatocytes was studied utilizing Nycodenz/sucrose gradients. It was proven in pulse chase experiments that the ligand endocytosed initially (after half of to 1 min) was in small, slow-sedimenting vesicles of comparable sizes. The vesicles containing the ligand elevated in measurement, and after about 2.5 min 20-30% of the …. Separation of endocytic vesicles in Nycodenz gradients. Read More ». ...

GAL3ST4 - Galactose-3-O-sulfotransferase 4; Catalyzes the transfer of sulfate to beta-1,3-linked galactose residues in O-linked glycoproteins. Good substrates include asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc; Sulfotransferases, membrane bound [a.k.a. PP6968, ENSP00000398304, ENSP00000353142, ENSP00000292377] ...

Asialoglycoprotein Receptor 1兔多克隆抗体(ab42488)可与大鼠, 人样本反应并经WB, ICC/IF实验严格验证，被2篇文献引用并得到2个独立的用户反馈。

TY - JOUR. T1 - Purification and characterization of human liver β-galactosidase from a patient with the adult form of GM1 gangliosidosis and a normal control. AU - Muto, Tatsuro. AU - Naoi, Makoto. AU - Nagatsu, Toshiharu. AU - Takahashi, Akira. AU - Matsuoka, Yukihiko. AU - Hashizume, Yoshio. AU - Fujiki, Norio. PY - 1988/2/17. Y1 - 1988/2/17. N2 - β-Galactosidases were purified to homogeneity from livers of a normal control and a patient with the adult form of GM1 gangliosidosis. The purification was achieved by chromatography on DEAE-Sepharose fast flow, Con A-Sepharose, p-aminophenyl-1-thio-β-d-galactopyrarose, and QAE-Mono Q. The normal and mutant enzymes were purified about 5000-fold with a yield of 10% and 1800-fold with a yield of 34%, respectively, and could hydrolyze 4-methylumbelliferyl-β-d-galactoside, GM1 ganglioside, and asialofetuin. The purified normal enzyme was eluted from a TSK gel G-4000SW column as three symmetrical peaks of protein which were coincident with the three ...

TY - JOUR. T1 - Influence of residualizing properties of the radiolabel on radionuclide molecular imaging of HER3 using affibody molecules. AU - Rinne, Sara S.. AU - Xu, Tianqi. AU - Leitao, Charles Dahlsson. AU - Ståhl, Stefan. AU - Löfblom, John. AU - Orlova, Anna. AU - Tolmachev, Vladimir. AU - Vorobyeva, Anzhelika. N1 - Funding Information: Funding: This research was funded by the Swedish Cancer Society (grants CAN 2017/425 (A.O.), CAN 2018/436 (V.T.), CAN 2017/649 (J.L.) and CAN 2016/463 and CAN 2019/190101 (S.S.)), the Swedish Research Council (grants 2015-02509 (A.O.), 2019-00994 (V.T.)), the Swedish Agency for Innovation VINNOVA (2019/00104 (J.L.)) and the Wallenberg Center for Protein Technology (S.S. and J.L.). Funding Information: Acknowledgments: The molecular imaging work in this publication was supported by the Wallenberg infrastructure for PET-MRI (WIPPET) at SciLifeLab Pilot Facility for Preclinical PET-MRI, a Swedish nationally available imaging platform at Uppsala University, ...

A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates the endocytosis of serum glycoproteins.

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The role of the two galactose binding sites of ricin B chain in ricin toxicity was evaluated by studying a series of ricin point mutants. Wild-type (WT) ricin and three ricin B chain point mutants having mutations in either 1) the first galactose binding domain (site 1 mutant, Met in place of Lys-40 and Gly in place of Asn-46), 2) the second galactose binding domain (site 2 mutant, Gly in place of Asn-255), or 3) both galactose binding domains (double site mutant containing all three amino acid replacements formerly stated) were expressed in Xenopus oocytes and then reassociated with recombinant ricin A chain. The different ricin B chains were mannosylated to the same extent. Cytotoxicity of these toxins was evaluated when cell entry was mediated either by galactose-containing receptors or through an alternate receptor, the mannose receptor of macrophages. WT ricin and each of the single domain mutants was able to kill Vero cells following uptake by galactose containing receptors. Lactose ...

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1FIH: Mechanism of pH-dependent N-acetylgalactosamine binding by a functional mimic of the hepatocyte asialoglycoprotein receptor.

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sialyltransferase 7 ((alpha-N-acetylneuraminyl 2,3-betagalactosyl-1,3)-N-acetyl galactosaminide alpha-2,6-sialyltransferase) D ...

Ficolins are a group of proteins mainly consisting of collagen-like and fibrinogen-like domains and are thought to play a role in innate immunity via their carbohydrate-binding activities. Two types of ficolins have been identified in mice, ficolin A, and ficolin B. However, their structure and func …

The incorporation of carbohydrate functional elements in the architecture of polycationic amphiphilic cyclodextrins (paCDs) provides glycosylated paCDs (pGaCDs) that form transfectious nanocomplexes (glycoCDplexes) with pDNA. In this study, we aimed at elucidating the internalization mechanisms at play and their incidence in transfection efficiency for glycoCDplexes formulated with 6-amino-6-deoxy-β-d-glucopyranosyl-appended pGaCDs in comparison with mannosylated and non-glycosylated congeners. Preliminary data showed a relatively high uptake of the 6-aminoglucosylated nanocomplexes by BNL-CL2 hepatocytes that correlated with a strong affinity towards the galactose-specific peanut agglutinin (PNA) lectin, suggesting that the galactose-binding asialoglycoprotein receptor at the surface of hepatocytes might be involved in glycoCDplex internalization. Transfection kinetics, internalization rates and protein expression data in BNL-CL2 ASGPR-expressing cells and COS-7 ASGPR-devoid epithelial cells ...

This study highlights that in hepatocytes, SARS-CoV-2 spike protein binding probably involved other receptors and mechanisms than only ACE-2.

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ST8SIA2 (ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.

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Incubation of HeLa cells in the presence of millimolar concentrations of propionate, butyrate, or pentanoate increases the specific activity of CMP-sialic acid:lactosylceramide sialyltransferase 7-20-fold within 24 h. Longer-chain saturated fatty acids or acetate are much less effective, decanoate showing no induction. Unsaturated fatty acid analogs of butyrate and other compounds are ineffective. Only the three most effective compounds also produce characteristic smooth extended cell processes in HeLa cells. Butyrate (5 mM) induces the sialyltransferase after a 4-h lag, producing maximum specific activity by 24 h. The amount of sialyl-lactosylceramide, the glycolipid product of the enzyme, increases during that time 3.5 times more than in control cultures. No other glycosphingolipid enzyme is significantly altered by butyrate exposure. The cellular shape changes occur 2-3 h later than the increase of sialyltransferase activity, and both processes require the continuous presence of inducer and ...

1FIF: Mechanism of pH-dependent N-acetylgalactosamine binding by a functional mimic of the hepatocyte asialoglycoprotein receptor.

Looking for online definition of Beta-galactoside-binding lectin L-14-II in the Medical Dictionary? Beta-galactoside-binding lectin L-14-II explanation free. What is Beta-galactoside-binding lectin L-14-II? Meaning of Beta-galactoside-binding lectin L-14-II medical term. What does Beta-galactoside-binding lectin L-14-II mean?

TY - JOUR. T1 - Asialoglycoprotein receptor phosphorylation and receptor-mediated endocytosis in hepatoma cells. Effect of phorbol esters. AU - Fallon, R. J.. AU - Schwartz, A. L.. PY - 1988/1/1. Y1 - 1988/1/1. N2 - The asialoglycoprotein (ASGP) receptor on Hep G2 cells undergoes constitutive recycling and ligand endocytosis in the presence of phorbol dibutyrate, at a 50% reduced rate relative to control cells (Fallon, R. J., and Schwartz, A. L. (1986) J. Biol. Chem. 261, 15081-15089). The relevance of receptor phosphorylation to these events was investigated by selective immunoprecipitation of surface receptors with polyclonal anti-human ASGP antiserum and pulse-chase labeling with [32P]orthophosphate to identify subcellular locations of initial receptor phosphorylation events as well as the eventual fate of phosphorylated receptor during recycling. The surface immunoprecipitation method recovers greater than 95% of surface ASGP receptors and only 5% or less of intracellular ...

The major and minor forms of the asialoglycoprotein receptor (ASGP-R), designated in the rat as RHL-1 and RHL-2/3, respectively, have traditionally been considered to be expressed exclusively in hepatic parenchymal cells. Northern blot analysis now demonstrates that rat and mouse testis express a receptor similar to the RHL-1 ASGP-R but not the RHL-2/3 receptor. In situ hybridization studies demonstrate that late-stage mouse and rat spermatids are the testicular cells that express the RHL-1 ASGP-R. The rat spermatid RHL-1 receptor has functional binding capability, with a KD of approximately 1.4 x 10(-8) M, and similar characteristics, compared with the hepatic RHL-1 receptor, regarding ligand binding specificity and ion dependence. These findings have major implications for therapeutic procedures attempting to target cytotoxic agents or DNA to hepatocytes using the ASGP-R. In addition, these findings demonstrate that late-stage spermatids are transcriptionally active and suggest that the RHL-1 ...

LGALS1, beta-galactoside-binding lectin,14 kDa lectin,14K beta-galactoside-binding lectin,C-14,galectin CG-1B,gbl,lectin, galactoside-binding, soluble, 1 (galectin 1 ...

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