Ferritin has been purified from normal full-term human placentae and its antigenic and molecular characteristics compared with adult liver ferritin. Placental ferritin is composed predominantly of a single subunit type, co-migrating with a liver ferritin standard on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. Comparison of dose-response curves in an immunoradiometric assay indicated some tissue-specific antigenicity for placental ferritin. This was supported by immunofluorescence studies on cryostat sections of human placentae by using antibodies to placental and spleen ferritin. Specific staining for placental ferritin was demonstrated within placental syncytiotrophoblast, particularly localized towards the microvillus plasma membrane. Ferritin has also been shown by electrophoretic and antigenic analysis to be present in protein fractions solubilized from isolated human syncytiotrophoblast microvillus plasma-membrane preparations, suggesting that ferritin may play an active ...

BACKGROUND: The expression of the two types of ferritin subunits, the H-subunit and L-subunit, has been shown to be differentially regulated by cytokines. The primary aim of the present study was to quantitatively measure the expression of the H-subunit and L-subunit of ferritin in bone marrow macrophages and cells of the erythron in patients with chronic T-helper cell type-1 immune stimulation. METHODS: The expression of the H-subunit and L-subunit of ferritin in bone marrow macrophages and cells of the erythron was quantitatively evaluated by post-embedding immunolocalisation with immunogold transmission electron microscopy. RESULTS: The present study showed up-regulation of the H-subunit of ferritin in the bone marrow macrophage in patients with pronounced cellular immune activation (94.7 ± 37.3 counts/μm2; n = 31 vs 72.4 ± 34.0 counts/μm2; n = 13, p-value = 0.037). CONCLUSION: This supports a possible role for H-subunit rich ferritins in the hypoferraemia of chronic disease ...

Ferritins (MW: ~ 450 kD) are polymeric proteins composed of 2 subunits a heavy chain and a light chain polypeptide, that reversibly bind and store iron in liver, spleen and bone marrow. The ferritin molecule consists of 24 self-assembling polypeptide subunits composed of the heavy and light chains. Ferritin light chain is the main intracellular iron storage protein. The proportion of heavy and light chain composing the ferritin molecule varies from tissue to tissue. This variation accounts for the differences in the rate of iron uptake and release in different tissues or organs. Recombinant Human Ferritin Light Chain is produced in E.coli as a single non-glycosylated polypeptide having a molecular weight of 19 kDa. Clinically, the monitoring of serum ferritin levels aides in the diagnosis and management of iron metabolic disorders. Serum levels increase in hepatic diseases, various malignancies, inflammation and late stage hemochromatosis. Ferritin levels are also indicative of the ero

Ferritin is the intracellular protein responsible for the sequestration, storage and release of iron. Ferritin can accumulate up to 4500 iron atoms as a ferrihydrite mineral in a protein shell and releases these iron atoms when there is an increase in the cells need for bioavailable iron. The ferritin protein shell consists of 24 protein subunits of two types, the H-subunit and the L-subunit. These ferritin subunits perform different functions in the mineralization process of iron. The ferritin protein shell can exist as various combinations of these two subunit types, giving rise to heteropolymers or isoferritins. Isoferritins are functionally distinct and characteristic populations of isoferritins are found depending on the type of cell, the proliferation status of the cell and the presence of disease. The synthesis of ferritin is regulated both transcriptionally and translationally. Translation of ferritin subunit mRNA is increased or decreased, depending on the labile iron pool and is ...

Ferritins are ubiquitous and can be found in practically all organisms that utilize Fe. They are composed of 24 subunits forming a hollow sphere with an inner cavity of ~80 A in diameter. The main function of ferritin is to oxidize the cytotoxic Fe(2+) ions and store the oxidized Fe in the inner cavity. It has been established that the initial step of rapid oxidation of Fe(2+) (ferroxidation) by H-type ferritins, found in vertebrates, occurs at a diiron binding center, termed the ferroxidase center. In bacterial ferritins, however, X-ray crystallographic evidence and amino acid sequence analysis revealed a trinuclear Fe binding center comprising a binuclear Fe binding center (sites A and B), homologous to the ferroxidase center of H-type ferritin, and an adjacent mononuclear Fe binding site (site C). In an effort to obtain further evidence supporting the presence of a trinuclear Fe binding center in bacterial ferritins and to gain information on the states of the iron bound to the trinuclear ...

When ferritin is reconstituted from Fe and apoferritin in vitro in the presence of Pi, the product obtained differs both from native ferritin and from ferritin reconstituted in the absence of Pi. When the latter is incubated with Pi the product resembles native ferritin with respect both to the pattern of Pi incorporated per molecule or per Fe atom and to the ease of release of this Pi relative to Fe release. It is concluded that much of the Pi of native ferritin is adsorbed on surfaces of ferritin iron-core crystallites. The results also suggest that Pi is not present at the intracellular site of Fe incorporation into ferritin, but is added after Fe. ...

Ferritin is a major iron storage protein and essential for iron homeostasis. It has a wide range of functions in the body including iron delivery, immunosuppression, angiogenesis, and cell proliferation. Ferritin is overexpressed in many cancer cells, but its precise role in cancer is unclear. In the present study, we examined the functional roles of ferritin in protecting the MCF-7 breast cancer cell line against treatment with the chemotherapeutic agent doxorubicin. The effects of ferritin (human liver ferritin) and doxorubicin on the human MCF-7 breast cancer cell line were evaluated using the cell viability assay. The impact of decreasing ferritin light chain (FTL) and ferritin heavy chain (FTH) expression on doxorubicin sensitivity was assessed using siRNA. Reactive oxygen species (ROS) was also measured using the fluorescence probe CM-H2DCFDA. The mechanism of modulated chemosensitivity was evaluated by western blot analysis. Ferritin treatment activated MCF-7 cell proliferation in a ...

Define iron-storage disease. iron-storage disease synonyms, iron-storage disease pronunciation, iron-storage disease translation, English dictionary definition of iron-storage disease. Noun 1. iron-storage disease - pathology in which iron accumulates in the tissues; characterized by bronzed skin and enlarged liver and diabetes mellitus...

TY - JOUR. T1 - Effect of 40-cm segment umbilical cord milking on hemoglobin and serum ferritin at 6 months of age in full-term infants of anemic and non-anemic mothers. AU - Bora, R.. AU - Akhtar, S. S.. AU - Venkatasubramaniam, A.. AU - Wolfson, Julian. AU - Rao, Raghu. PY - 2015/10/1. Y1 - 2015/10/1. N2 - Objective: To assess the effect of early clamping and milking of a 40-cm umbilical cord LUCM (long umbilical cord and milking) on hemoglobin (Hb) and serum ferritin concentrations at 6 months of age and to evaluate whether the effect is different in infants of anemic and non-anemic mothers. Study design: Eligible term-infants of anemic (maternal Hb,11.0 g dl-1) and non-anemic mothers (Hb ≥11.0 g dl-1) were randomized to LUCM or control groups (N=100 each). In the LUCM group, the umbilical cord was clamped at 40-cm length and milked. The control group had the cord clamped at 5 cm and not milked. Neonatal morbidities until discharge and Hb and serum ferritin at 6 months of age were compared. ...

Acute administration of iron to rats has been previously shown to induce liver ferritin synthesis by increasing the translation of inactive cytoplasmic ferritin mRNAs for both heavy (H) and light (L) subunits by mobilizing them onto polyribosomes. In this report rat hepatoma cells in culture are used to explore the relationship of this response to intracellular iron levels. After adding iron as ferric ammonium citrate to the medium, latent ferritin H- and L-mRNAs were extensively transferred to polyribosomes, accompanied by increased uptake of [35S]methionine into ferritin protein. Because total cellular levels of L- and H-mRNA were not significantly changed by exposure to iron, the increased ferritin mRNAs on polyribosomes most probably come from an inactive cytoplasmic pool, consistent with the inability of actinomycin-D and of cordycepin to inhibit iron-induced ferritin synthesis. When deferoxamine mesylate, an intracellular iron chelator, was added after the addition of iron to the medium, ferritin

Ferritin is a universal intracellular protein that stores iron and releases it in a controlled fashion. The protein is produced by almost all living organisms, including algae, bacteria, higher plants, and animals. In humans, it acts as a buffer against iron deficiency and iron overload. Ferritin is found in most tissues as a cytosolic protein, but small amounts are secreted into the serum where it functions as an iron carrier. Plasma ferritin is also an indirect marker of the total amount of iron stored in the body, hence serum ferritin is used as a diagnostic test for iron-deficiency anemia. Ferritin is a globular protein complex consisting of 24 protein subunits forming a nanocage with multiple metal-protein interactions. It is the primary intracellular iron-storage protein in both prokaryotes and eukaryotes, keeping iron in a soluble and non-toxic form. Ferritin that is not combined with iron is called apoferritin. Ferritin genes are highly conserved between species. All vertebrate ferritin ...

Published in Review of Diabetic Studies, Volume 5, Issue 4, Winter 2008, pages 245-252. Le, T.D., Bae, S., Hsu, C.E., Singh, K.P., Blair, S.N., Shang, N. (2009) Effects of Cardiorespiratory Fitness on Serum Ferritin Concentration and Incidence of Type 2 Diabetes: Evidence from the Aerobics Center Longitudinal Study (ACLS). Review of Diabetic Studies; 5:245-52.. © Review of Diabetic Studies 2009, The Society for Bio medical Diabetes Research ...

TY - JOUR. T1 - H and L ferritins in myocardium in iron overload. AU - Arbustini, Eloisa. AU - Grasso, Maurizia. AU - Rindi, Guido. AU - Arosio, Paolo. AU - Gavazzi, Antonello. AU - Diegoli, Marta. AU - Bramerio, Manuela. AU - Levi, Sonia. AU - Barosi, Giovanni. PY - 1991/11/1. Y1 - 1991/11/1. N2 - Ferritin is an iron-containing protein composed of 2 subunits (H and L) with largely different primary structures and immunochemical properties. 1 The normal L-subunit-rich, basic isoferritin is prevalent in the liver and spleen, whereas the H-subunit-rich, acidic isoferritin predominates in the heart, pancreas and kidneys.2 A variation in heart isoferritin was reported in iron-overloaded patients,2 and the absence of the more acidic isoferritin was hypothesized to be specific for hereditary hemochromatosis.3 These observations prompted us to perform immunohistochemical investigations on H and L ferritins in endomyocardial biopsy samples of patients with primary or secondary iron overload, using ...

Iron is an essential element for the living organisms but free iron can be toxic. Iron metabolism disorders affect a large portion of human population. There exists a need for development of a reference technique to measure body iron stores. The ideal technique for determination of body iron stores would to measure ferritin concentration along with ferritin iron saturation. This study uses the principle of Isotope Dilution Mass Spectrometry (IDMS) to measure serum ferritin bound iron. IDMS requires the biosynthesis of a ferritin spike with isotopically enriched iron and sulphur. The aim of this project was to synthesize a ferritin spike for serum ferritin and ferritin bound iron quantification for development of a reference technique to determine body iron stores. This study involved the biosynthesis of a ferritin spike labelled with isotopic iron and sulphur ...

In adults, ZPP/H is inversely correlated with plasma ferritin,12,14 and inversely related to the amount of stainable iron in the marrow.13 We observed a fall in plasma ferritin in the first year of life, which agrees with the known changes in body iron stores in neonates.11,19 However, rather than seeing the expected increase in ZPP/H during this period, a decrease was seen. Indeed ZPP/H and ferritin were directly, not inversely, related. When the entire dataset was examined using multiple regression, with age included in the model, there was no relation between log (ZPP/H) and log (ferritin). However, there was an inverse relation between average ZPP/H residuals for each subject, from a multiregression model, and corresponding log (ferritin) residuals. This suggests that subjects with a higher than average plasma ferritin tend to have lower ZPP/H (the between subject correlation). However, the relation was relatively weak (r = −0.22). When a similar model was used that incorporated subject, ...

CAD is a multifactorial chronic disease that develops from the interplay of genetic, lifestyle, physiological and other factors; a well understanding of the interaction between these risk factors is important to identify the target groups for CAD. In our previous study,20 serum ferritin was found to be an independent risk factor of CAD, and several studies21 have reported that obesity could elevate serum ferritin levels. However, whether there is an interaction of BMI with serum ferritin levels and CAD risk, and the extent to which the interaction influences the CAD risk are still unknown, and no study has reported the biological interaction of serum ferritin and BMI with CAD risk so far. Thus, in the present study, we explored the possible biological interaction of serum ferritin levels and BMI with CAD risk, and found evidence of additive interaction. This finding provided further evidence that iron overload to the risk of CAD appeared to be most significant among persons with higher ...

Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Cadmium atom in PDB 1ier: Cubic Crystal Structure of Native Horse Spleen Ferritin

Ferritin is a protein in the body that stores iron and releases it. A simple blood test can determine this value for you. When Ferritin builds up, iron overload is the result. Over time, these excesses build up in major organs and if it is not removed, these organs can become diseased. This can be a genetic trait, in which it is called Hemochromatosis. However, this is the case only about 10% of patients with high Ferritin. Elevated Ferritin is a lot more common for other reasons than you think and is to not be overlooked. Untreated hemochromatosis can be fatal. More often though, the iron overload presents in patients with obesity, chronic inflammation, daily alcohol consumption, heart disease, liver disease, renal failure, joint paint, metabolic syndrome or diabetes, or even cancer.. Dr. Paul Adams of division of Gastroenterology University of Western Ontario, Canada says it is important to remember that elevated ferritin does not equal iron overload and there are many patients with elevated ...

Serum ferritin is a sensitive indicator of available iron stores (1), but in certain instances it cannot be used in diagnosis, e.g., in anemias of chronic disease, infections, inflammation, liver disease, and malignancies (2)(3)(4)(5)(6)(7). Iron stores may be normal or increased, though accompanied by increased serum ferritin, in anemias of chronic disorders, aplastic anemia, sideroblastic anemia, and chronic hemolytic anemia. Because ferritin is also a positive acute-phase reactant protein that is increased in inflammation (2), serum ferritin concentration is not a reliable index of available iron stores in individuals with chronic diseases. There is no information, however, on whether ferritin can be used as a marker of available iron stores in the anemia of copper deficiency.. Unlike iron-deficiency anemia, in which body iron stores are usually depleted as evidenced by diminished serum ferritin concentrations, anemia of copper deficiency (8)(9)(10) results from increased hepatic iron stores ...

Ferritin cores from human spleen, limpet (Patella vulgata) haemolymph and bacterial (Pseudomonasaeruginosa) cells have been investigated using 57Fe Mössbauer spectroscopy. The Mössbauer spectra were recorded over a range of temperatures from 1.3 to 78 K. At 78 K, all the spectra are quadrupole-split doublets with similar quadrupole splittings and isomer shifts, characteristic of iron(III), while at sufficiently low temperatures the spectra of all the samples show well-resolved magnetic splitting. At intermediate temperatures, the spectra from the human ferritin exhibit typical superparamagnetic behaviour, while those from the bacterial ferritin show behaviour corresponding to a transition from a magnetically ordered to a paramagnetic state. The spectra from the limpet ferritin show a complex combination of the two effects. The results are discussed in terms of the magnetic behaviour of small particles. The data are consistent with magnetic ordering temperatures of about 3 and 30 K for the ...

The power to study diabetes in men was low. This could be one of the reasons why the association between iron status and diabetes was only significant in women. Serum ferritin is the most commonly used marker of stored body iron (15). However, serum ferritin is positively and hemoglobin is negatively associated by inflammation and infection (16). We do not have information on C-reactive protein, which is often used as an indicator of inflammation or infection. The association between hemoglobin and serum ferritin may thus be confounded by these conditions. In our results, we found a high prevalence of anemia (18.3 and 31.5%, for men and women, respectively), but the percentage having serum ferritin ,15 μg/l was only ∼9%. The prevalence of iron deficiency anemia was thus low: 6.3% among women and 0.7% among men. Thalassemia is not a problem in this area, with a prevalence of 0.09% (17).. It is reported that the prevalence of the hepatitis B virus marker is 42.6% and hepatitis B surface antigen ...

Elevated ferritin levels in a blood test can mean an individual has hemochromatosis, an infection, Hodgkins disease leukemia, inflammatory conditions or a diet too high in iron, according to WebMD....

Ferritin was measured in sera obtained at diagnosis from 241 patients with neuroblastoma to determine (a) the incidence of elevated ferritin and (b) the relationship between ferritin level and outcome. Ferritin was infrequently elevated in sera from patients with Stages I and II disease but was abnormally elevated in 37 and 54% of those with Stages III and IV neuroblastoma, respectively. The mean and median levels for each stage were compared and were highest for Stages III and IV disease. Analysis of progression-free survival for children with Stages III and IV disease indicated that elevated ferritin was associated with a significantly poorer prognosis than was normal ferritin and that this correlation was independent of stage and age at diagnosis. Progression-free survival at 24 months of follow-up for patients with Stage III disease with normal ferritin was 76% and with elevated ferritin was 23%. For those with Stage IV disease, progression-free survival was 27 and 3% with normal and ...

TY - JOUR. T1 - Serum ferritin as a clinical marker for renal cell carcinoma. T2 - influence of tumor volume. AU - Partin, Alan Wayne. AU - Criley, Stuart R.. AU - Steiner, Mitchell S.. AU - Hsieh, Kisseng. AU - Simons, Jonathan W.. AU - Lumadue, Jeanne. AU - Carter, H Ballentine. AU - Marshall, Fray F.. PY - 1995. Y1 - 1995. N2 - Objectives. At present, 35% to 50% of patients with clinically localized renal cell carcinoma (RCC) unpredictably have a recurrence after surgical therapy. Presently, no clinical serum marker exists to detect occult metastases and to allow measurement of response to therapy in RCC. Serum ferritin was previously reported to correlate with pathologic stage. We postulated that this increase in serum ferritin with increasing stage might reflect tumor volume, since higher stage tumors are often larger. Methods. Serum ferritin levels were measured preoperatively in 30 patients with radiologic evidence of RCC. Tumor volume and the largest tumor dimension were calculated from ...

Altitude exposure (mean ± s: 21 ± 3 days) increased Hbmass by 1.1% [-0.4, 2.6], 3.3% [1.7, 4.8], and 4.0% [2.0, 6.1] from pre-altitude levels in athletes who ingested nil, 105 mg and 210 mg respectively, of oral iron supplement daily. Serum ferritin levels decreased by -33.2% [-46.9, -15.9] and 13.8% [-32.2, 9.7] from pre-altitude levels in athletes who supplemented with nil and 105 mg of oral iron supplement daily, but increased by 36.8% [1.3, 84.8] in athletes supplemented with 210 mg of oral iron daily. Finally, athletes who ingested either 105 mg or 210 mg of oral iron supplement daily had a greater TII compared with non-supplemented athletes (0 versus 105 mg: effect size (d) = -1.88 [-2.56, -1.17]; 0 versus 210 mg: effect size (d) = -2.87 [-3.88, -1.66 ...

Previous studies have shown that lenticular levels of Fe and Cu are elevated in age-related cataract. However, it is not known if these metals are present in a state that is permissive for redox reactions that may lead to the formation of free radicals. In addition, there is little data available concerning the concentration and lenticular distribution of ferritin, the major intracellular Fe-sequestering protein, in the lens. The aim of the present work was therefore to determine the distribution of ferritin and the redox-availability of Fe and Cu in healthy and cataractous lenses. Lens ferritin distribution was assessed by ELISA and immunohistochemistry. A modified ELISA detected ferritin in an insoluble lens protein fraction. Ferritin levels were not significantly different in the cortex vs nucleus of healthy lenses. In contrast, ferritin levels in the cataractous lens nuclei appeared to be 70 % lower compared to the cortex. This was at least partially due to the presence of ferritin within ...

The utility of plasma or serum ferritin in screening for iron deficiency in relatively healthy patients is well established (2)(3)(4) and is further supported by our results in the medical student population. However, directed evaluation of the cause of anemia in ill patients, particularly those with inflammatory diseases, can be difficult because of the acute-phase reactant properties of ferritin. In such patients, the diagnostic sensitivity of ferritin for iron deficiency can be compromised, and either bone marrow biopsy or determination of the response to iron therapy is required to completely assess iron status. sTfR concentration increases with iron deficiency and other causes of increased erythropoiesis but is not affected by acute or chronic inflammatory illnesses (8)(9)(10)(11). Accordingly, sTfR has been suggested as a highly sensitive laboratory test for iron deficiency (12)(13)(21). However, just as acute or chronic illnesses may yield falsely negative results for ferritin, other ...

Ferritins are recognized as key players in the iron storage and detoxification processes. Iron acquisition in the case of pathogenic bacteria has long been established as an important virulence mechanism. Here, we report a 3.0 Å crystal structure of a ferritin, annotated as Bacterioferritin B (BfrB), from Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis that continues to be one of the worlds deadliest diseases. Similar to the other members of ferritin family, the Mtb BfrB subunit exhibits the characteristic fold of a four-helical bundle that possesses the ferroxidase catalytic centre. We compare the structure of Mtb BfrB with representatives of the ferritin family belonging to the archaea, eubacteria and eukarya. Unlike most other ferritins, Mtb BfrB has an extended C-terminus. To dissect the role of this extended C-terminus, truncated Mtb BfrB was purified and biochemical studies implicate this region in ferroxidase activity and iron release in addition to providing stability to

Iron sequestration by host iron-binding proteins is an important mechanism of resistance to microbial infections. Inside oral epithelial cells, iron is stored within ferritin, and is therefore not usually accessible to pathogenic microbes. We observed that the ferritin concentration within oral epithelial cells was directly related to their susceptibility to damage by the human pathogenic fungus, Candida albicans. Thus, we hypothesized that host ferritin is used as an iron source by this organism. We found that C. albicans was able to grow on agar at physiological pH with ferritin as the sole source of iron, while the bakers yeast Saccharomyces cerevisiae could not. A screen of C. albicans mutants lacking components of each of the three known iron acquisition systems revealed that only the reductive pathway is involved in iron utilization from ferritin by this fungus. Additionally, C. albicans hyphae, but not yeast cells, bound ferritin, and this binding was crucial for iron acquisition from ...

Iron is an essential component of life. However, iron is also a potent biological toxin. Thus the bioavailability of iron must be stringently regulated. At the cellular level, if there is too little iron, a cell will lack the ability to meet its metabolic and biosynthetic requirements. If there is too much iron the cell become vulnerable to oxidative stress. Iron requiring metabolic reactions are found in both nuclei and cytoplasm and molecules in both compartments are vulnerable to oxidative stress. Indeed, iron-binding sites have been reported on DNA and iron induced DNA damage is well established. In the cytoplasm, ferritin regulates iron bioavailability. Recently, we and two other groups have provided evidence that ferritin is present in the nuclei of some cells. By virtue of its ability to take up and release iron, ferritin may be uniquely suited to both deliver iron to the nucleus as well as provide protection from iron induced oxidative stress. Our preliminary data show that nuclear ...

The study was conducted in the Osborn Family Health Center, Our Lady of Lourdes Hospital, in Camden, New Jersey. Data were collected as part of the Camden Study, a prospective cohort study of maternal nutrition and pregnancy outcome in young, generally healthy women residing in one of the poorest cities in the continental U.S. (23). The institutional review board at the University of Medicine and Dentistry of New Jersey-SOM approved the study protocol. Informed written consent was obtained from each participant after explanation of the nature and purpose of the study.. Study participants were enrolled between October 1996 and June 2003 for prenatal care. Less than 5% of the women screened for eligibility were excluded from participation because of serious nonobstetric problem (e.g., lupus, type 1 or 2 diabetes, seizure disorders, malignancies, acute or chronic liver disease, drug or alcohol abuse, and psychiatric problems). Eighty percent of the patients who were eligible agreed to participate ...

Cellular uptake of ferritin amounting to 0-5 mug/mg cell protein or more can be measured colorimetrically on the basis of ferritin-iron content. 131I-serum albumin, soluble ferritin and aggregated ferritin used in equimolar concentrations are taken up differently by Sarcoma SI80 cells in culture. The net uptakes in 2 h at 37 degrees C are 0-065, 4-3 and 24-7 mug/mg cell protein or 0-93, 8-0 and 45-7 mumol, respectively. Albumin uptake is not inhibited by a 26-fold molar ferritin excess but is significantly inhibited by a 43-fold excess. The transport mechanism of the ferritins differs from that of albumin in that it is significantly inhibitable by 2 times 10(−4) M monoiodoacetate. Soluble ferritin contains small aggregates which are removed by filtration through Millipore membranes of 0-05, 0-1 and 0-22 mum. When the 0-1-mum filtrate is re-examined, uptake is no longer inhibited by iodoacetate. Since it can be inferred from other work that albumin is taken up by pinocytosis and ferritin ...

I was wondering if this has happened to anyone else...My husband has had over the past 18 months, more than 20 units of blood due to Chemo induced ANAEMIA...He is on his last cycle of gemzar/carbo platin treatment.... This week his blood reading came back with extremely high ferritin level of 3080.0 and our haemologist has recommended DEFERIPRONE (FERRITROX 270) in tablet form. The reason for the tablet form is because hubby has an ideal conduit and a colostomy ...so infusion in the belly

Plasma ferritin is an important extracellular iron storage molecule, whose concentration increases drastically in cancer and infection. During infection, the pathogen usurps host iron for its survival and pathogenicity; hence, maintenance of the plasma ferritin level during infection is a crucial host defence mechanism. In this study, the horseshoe crab plasma ferritin complex was purified, characterized, and its involvement in innate immune defence was investigated. The plasma ferritin appears as a 21-kDa subunit on SDS-PAGE. Full-length ferritin-H cDNAs (CrFer-H1 and CrFer-H2) were cloned. Analysis of the 5′ UTR indicates the existence of a functional iron-response element, suggesting that both the CrFer-H genes may be post-transcriptionally regulated. Northern analysis shows that the CrFer-H is ubiquitously expressed. Within 3 h of lipopolysaccharide challenge, the gene is up-regulated by > 12-fold. In contrast, iron-loading did not result in any significant change. When challenged with ...

Potential causes of high ferritin levels include liver disease, hyperthyroidism, rheumatoid arthritis and other inflammatory conditions, some forms of cancer, diabetes, multiple blood transfusions,...

I have high ferritin levels (over 800) but its not haemochromotosis. I recently had a bad fall (torn shoulder tendon) and had to get my shoulder operated on. I was prescribed strong pain killers (palexia). its called tapentadol. Ive stopped taking them as I worry theyre making me worse- but the pain persists. I am always so exhausted. I lie in bed all day. My joints hurt everywhere. My kids say ive become moody and irritable. im not sure what to do- my GP is struggling to assist. People tell me to go to emergency room but im not sure. Thank

Ferritin levels are important for everyone-whether youre male or female, an avid exerciser or a couch potato. If your ferritin levels are too low, you may feel fatigued and run down, which can be a problem for anyone, no matter their level of physical activity. To find out exactly what your ferritin levels are, sign up for an InsideTracker plan. If your levels are out-of-range, InsideTracker will give you suggestions for diet, exercise, supplement and lifestyle changes to help you optimize them.

Several novel findings on Drosophila ferritin are described in this work. We show that the absence of either Fer1HCH or Fer2LCH results in embryonic lethality and that modified Fer1HCH subunits (mutant in the ferroxidase center or GFP tagged) cannot substitute for lack of Fer1HCH. However, if the same modified subunits are expressed in the presence of wild-type subunits, they can be integrated into ferritin holomers without inducing dominant-negative effects. Analysis of heterozygous loss-of-function ferritin fly mutants or flies overexpressing ferritin subunits revealed that a constant ratio of Fer1HCH and Fer2LCH is maintained, independent of their internal transcriptional expression levels. The structural cooperation of the two subunits that is secured via disulfide bonds (Hamburger et al. 2005) likely explains these observations. A post-transcriptional mechanism, possibly involving the degradation of subunits that are present in excess, ensures the presence of equal amounts of the two ...

Previous studies have supported the theory that there is a positive association between ferritin and carotid atherosclerosis in Western people. Diet plays an important role in determining serum ferritin concentration. Asian dietary patterns are different from Western dietary patterns, implying that there may be a difference in the association of ferritin with carotid atherosclerosis between Asian and Western people. However, few studies focus on the association between ferritin and carotid atherosclerosis among Asians. The aim of this study was to investigate how serum ferritin levels are associated with carotid atherosclerosis in an Asian adult population. A cross-sectional assessment was performed in 8302 adults in Tianjin, China. Carotid intima-media thickness (IMT) and plaques were assessed using ultrasonography, and serum ferritin was measured using the protein chip-chemiluminescence method. Multiple logistic regression analysis was used to examine the association between quartiles of serum ...

Ferritin concentration was measured in cytosol extracts of 44 mammary carcinomas and 14 benign breast tissues. A six-fold difference was observed (mean, 364.6 +/- 223.3 ng/mcp in malignant tissue versus mean, 60.2 +/- 42.1 ng/mcp in benign tissue P less than 0.001). Thirty-five malignant tissue spec …

1. Measurements of T-lymphocyte surface ferritin using flow cytometry show that phytohaemagglutinin (PHA) stimulation causes a marked increase in the number of cells bearing spleen-type (S) and heart-type (H) ferritin on their membrane, whereas no such change occurs in non-stimulated cells. This coincides with increases in interleukin-2 receptors, transferrin receptors and HLA-DR antigen.. 2. There is an increase in the intracellular concentration of both S- and H-ferritin in lymphocytes after PHA stimulation: H-ferritin increases five- to seven-fold, but S-ferritin only two- to three-fold. The maximum H/S ratio is about 15/1. However, these increases also occur in cells cultured in the absence of PHA.. 3. Small amounts of both S- and H-ferritin are released into the medium, especially from stimulated cells, but the H/S ratios are lower than intracellular ratios.. 4. The present findings suggest that lymphocyte stimulation followed by ferritin synthesis is accompanied by an increase in the ...

None of the methods for assessing total body iron burden in patients with hemochromatosis is satisfactory. Although it is commonly believed that a relationship exists between serum ferritin levels and

Results: Apart from some minor gastrointestinal complaints reported in 3 BMT patients that did not require discontinuation of therapy, other side effects were not registered during the treatment. Subjectively, patients reported an improvement in the palatability of Jadenu® compared to Exjade® therapy in 8 out of 12 BMT patients. A non-significant decrease in LIC measured by Ferriscan® and serum ferritin levels was observed after one year of treatment with Jadenu®. A significant positive correlation was found between serum ferritin level and LIC measured by the FerriScan® method. LIC and serum ferritin level correlated significantly with ALT level (r = 0.31 and 0.45 respectively, p , 0.05). No significant correlation was detected between LIC and other biochemical or hormonal parameters.. Conclusion: Our study shows that short-term treatment with Jadenu® is safe but is associated with a non-significant decrease in LIC and serum ferritin levels. Therefore, there is an urgent need for ...

Ferritin is a protein that transports iron in your bodys tissues. Research has shown that ferritin is a very reliable indicator of the status of your bodys iron stores. People who have very low or very high ferritin levels may have conditions that produce fatigue as a symptom.

Goat anti human ferritin antibody recognizes Human Ferritin, a large globular protein found in the liver, intestines and spleen that funct

Ferritin, a major iron storage protein, is essential to iron homeostasis and is involved in a wide range of physiologic and pathologic processes. In clinical medicine, ferritin is predominantly utilized as a serum marker of total body iron stores. In cases of iron deficiency and overload, serum ferr …

Iranian Journal of Diabetes and Obesity is scientific quarterly journal published by Shahid Sadoughi University of Medical Sciences

Ferritin heavy chain is a ferroxidase enzyme that in humans is encoded by the FTH1 gene. This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. FTH1 has been shown to interact with Ferritin light chain. Ferritin GRCh38: Ensembl release 89: ENSG00000167996 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000024661 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Hentze MW, Keim S, ...

Although a partial ferritin subunit sequence of the seal Phoca vitulina (AF246195, unpublished data) has been determined, the entire coding sequences of ferritin subunits in marine mammals were previously unknown. The present study shows the ferritin coding sequences coding for the H and L subunits of six different dolphin species (P. crassidens, L. obliquidens, G. griseus, G. macrorhyncus, T. truncatus, and D. leucas). The predicted secondary structures using the GENETYX-MAC computer program (Robson method) of dolphin ferritin H and L subunits show A, B, C, D, and short E-helices and the L loop connecting B and C helices (data not shown) as in other mammalian species [1-3, 18]. Sequence comparisons between dolphin ferritin and that of other mammalian species strongly suggest similar functions for the H subunit unique ferroxidase [2, 3, 18], the L subunit salt bridge [2, 8, 23] and iron nucleation site [2, 8], subunit dimer formation [8, 18], and the iron channel [8, 18]. A putative ...

TY - JOUR. T1 - Serum ferritin, erythrocyte protoporphyrin and hemoglobin are valid indicators of iron status of school children in a malaria-holoendemic population. AU - Stoltzfus, Rebecca J.. AU - Chwaya, Hababu M.. AU - Albonico, Marco. AU - Schulze, Kerry J.. AU - Savioli, Lorenzo. AU - Tielsch, James M.. N1 - Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 1997/2. Y1 - 1997/2. N2 - In many African populations, the prevalences of both iron deficiency and malarial infection exceed 50%. The control of iron deficiency anemia is of urgent public health importance, but assessment of iron status in these contexts has been controversial because of the effects of malarial disease on common iron status indicators. We assessed iron status in 3605 school children in Zanzibar by measuring hemoglobin, erythrocyte protoporphyrin (EP) and serum ferritin concentrations. Malaria parasitemia was quantified by counting against leukocytes. Iron deficiency was highly prevalent: 62.4% of ...

Hereditary hyperferritinemia-cataract syndrome (HHCS) is an autosomal and dominant disease caused by heterogeneous mutations in the iron responsive element (IRE) of the 5 untranslated flanking region of ferritin L-chain mRNA, which reduce the binding to the trans iron regulatory proteins and make L-chain synthesis constitutively upregulated. In the several families identified so far, the serum and tissue L-ferritin levels are fivefold to 20- fold higher than in nonaffected control subjects, iron metabolism is apparently normal, and the only relevant clinical symptom is early onset, bilateral cataract. Some pathogenetic aspects of HHCS remain obscure, with particular reference to the isoferritins produced by HHCS cells, as well as the mechanism of cataract formation. We analyzed lymphoblastoid cell lines obtained from two nonaffected control subjects and from HHCS patients carrying the substitution A40G (Paris-1), G41C (Verona-1), and the deletion of the residues 10-38 (Verona-2) in the IRE ...

Mouse Ferritin light chain 1, Ftl1 ELISA KIT allows for the in vitro quantitative determination of Mouse Ftl1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Recombinant ferritin is an excellent template for the synthesis of magnetic nanoparticles. This paper describes carefully performed experiments both to identify ironoxides within nanoparticles and to measure the number of iron atoms in the cores of recombinant human H-chain ferritin (HFn), based on spectroscopy techniques. Using electron energy-loss spectroscopy (EELS) analysis, magnetite (Fe3O4) has been unequivocally identified as the ironoxide formed within HFn cores under special preparation conditions. Atom counting analysis by EELS and high-angle annular dark-field imaging further allowed the correlation of the particle sizes to the real Fe atom numbers in a quantitative manner. These results help clarify some structural confusion between magnetite and maghemite (γ-Fe2O3), and also provide standard data for the number of Fe atoms within Fe3O4 particles of a given size, whose use is not limited to cases of magnetite synthesized in the cores of recombinant human ferritin.

Reactions and interactions of iron and oxygen can be both beneficial and detrimental to cells and tissues. Iron is mainly found in our blood where it functions as a mediator in the transport of oxygen to the cells and is further vital for the cellular respiration reducing the oxygen to water. The flexible redox state of iron makes it ideal to contribute in single electron transfers, but may also catalyze reactions with oxygen resulting in cell damaging reactive oxygen species (ROS). Normally the cells are protected against iron toxicity by controlling iron uptake and storage. When the intracellular demand for iron increases; the iron uptake is promoted by increasing the expression of transferrin receptor (TfR) and by decreasing the expression of the iron storage protein ferritin. Ferritin has a central role in the cellular iron detoxification by keeping it in a non reactive but still bioavailable form. However, in neurodegenerative diseases like in Alzheimers and Parkinsons disease the iron ...

Background: Alopecia Areate (AA) is a recurrent non-scarring patchy hair loss of autoimmune origin in a genetically susceptible individual although environmental factors may be contributory. The aim of this study is to evaluate serum iron and ferritin levels in patients with AA. Methods: Fifty patients of AA and 50 age and sex matched control subjects without any hair loss were studied. Results: No significant difference was found in mean hemoglobin, ferritin, and iron levels in two groups. Serum iron ferritin and hemoglobin levels did not show any significant variations with respect to the extent or duration of the disease, family history, history of atopy, and nail involvement. Conclusion: In our study, the levels of serum iron and ferritin showed no significant changes in AA patients and seems not to play a role in the etiology of AA.

Background: High body iron store has been associated with an increased risk of type 2 diabetes (T2D); it remains unknown whether the genetic variants related to body iron status affect T2D risk. We aimed at comprehensively investigating the associations between the genetic variants related to body iron status and the T2D risk. Methodology/Principal Findings Six common SNPs related to body iron status from recent genome-wide association (GWA) studies were determined in the Nurses Health Study (NHS; 1,467 diabetic cases and 1,754 controls) and the Health Professionals Follow-up Study (HPFS; 1,124, diabetic cases and 1,298 controls). Plasma levels of ferritin, soluble transferrin receptor (sTfR), and transferrin were measured in NHS. Significant associations were observed for loci in TPMRSS6 with sTfR (P = 3.47×10−6), TF with transferrin (P = 0.0002 to 1.72×10−10); and HFE with ferritin (P = 0.017 to 1.6×10−8), sTfR (P = 0.007 to 7.9×10−6), and transferrin (P = 0.006 to 0.0007). The ...

Methods currently used to determine iron bioavailability have disadvantages for both the subjects and researchers involved. One safe and simple method that needs further evaluation, the serum iron absorption method, involves an initial blood drawing, dosage s of iron, and blood drawings taken at intervals thereafter. Generally, the rise in serum iron or area under the curve is used to determine iron uptake. Two experiments were conducted using the serum iron absorption method in an effort to improve the utility of this method for the measurement of iron bioavailability. With this effort in mind, an equation adjusting for dose of iron (0.5-8 mg) and blood volume was used to estimate serum iron absorption and allow for adequate comparisons of other iron absorption data obtained with this method and others. One very significant result was that low serum ferritin was found to be a predictor of high serum estimated iron absorption (SEIA). Similarly, low serum ferritin levels were also highly correlated to

Results: Sixteen of the subjects (0.5 percent) were homozygous for the C282Y mutation, and 424 (14.1 percent) were heterozygous. The serum transferrin saturation was 45 percent or higher in 15 of the 16 who were homozygous; in 1 subject it was 43 percent. Four of the homozygous subjects had previously been given a diagnosis of hemochromatosis, and 12 had not. Seven of these 12 patients had elevated serum ferritin levels in 1994; 6 of the 7 had further increases in 1998, and 1 had a decrease, although the value remained elevated. The serum ferritin levels in the four other homozygous patients remained in the normal range. Eleven of the 16 homozygous subjects underwent liver biopsy; 3 had hepatic fibrosis, and 1, who had a history of excessive alcohol consumption, had cirrhosis and mild microvesicular steatosis. Eight of the 16 homozygous subjects had clinical findings that were consistent with the presence of hereditary hemochromatosis, such as hepatomegaly, skin pigmentation, and arthritis ...

Dr Lal PathLabs SOLUBLE TRANSFERRIN RECEPTOR (STFR), SERUM in Bangalore is health checkup package includes 1 Tests. ₹100 Cashback and Free Doctor Consultation, Home sample collection and Online reports available. SOLUBLE TRANSFERRIN RECEPTOR (STFR), SERUM in Bangalore covers 1 parameters- SOLUBLE TRANSFERRIN RECEPTOR (STFR), SERUM in Bangalore

TY - JOUR. T1 - Do oligodendrocytes mediate iron regulation in the human brain?. AU - Gerber, Megan R.. AU - Connor, James R.. PY - 1989/7. Y1 - 1989/7. N2 - We used immunohistochemical studies to demonstrate that transferrin (the iron mobilization protein) and ferritin (the iron storage protein) are specifically localized in oligodendrocytes in gray and white matter of the human central nervous system. In addition, iron is also localized predominantly in oligodendrocytes. Oligodendrocytes have been well established as the cells responsible for myelin production in the central nervous system. The results of this study suggest that oligodendrocytes (or a subpopulation of oligodendrocytes) might have the additional function of mediating iron mobilization and storage in the central nervous system.. AB - We used immunohistochemical studies to demonstrate that transferrin (the iron mobilization protein) and ferritin (the iron storage protein) are specifically localized in oligodendrocytes in gray and ...

Read on Correlation of Serum Ferritin Levels with Liver Function Tests and Anthropometric Measurements in Transfusion Dependent Beta-Thalassemia Major Children: A Cross Sectional Study.

Diazymes Ferritin Assay provides an effective test method for laboratories of virtually all sizes, said Dr. Chong Yuan, Managing Director of Diazyme Laboratories. The sensitivity of the assay allows ferritin levels to be measured very efficiently and effectively. This high-throughput liquid stable assay provides precise test results, is user friendly, cost effective and has excellent correlation to the existing commercial methods. Diazyme Laboratories is a division of General Atomics headquartered in La Jolla, California. Diazyme uses its enzyme platform technologies to develop innovative assays for clinical and research uses with reduced costs and improved performance. Products include diagnostic blood tests for cardiac markers, diabetes, nutritional assessment, liver disease, renal disease and electrolytes. Information regarding Diazymes enzyme technology and related products can be found on its website at www.diazyme.com. ...

Hi everyone, I have had my blood test results. Please could anyone tell me if these results are ok for someone with rls - Serum ferritin level (XE24r) 56ug/L [30.0 - 400.0] Gobblededook! Receptionist...

Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...

Parahita Diagnostic Centre, Berpangalaman belasan tahun dengan ratusan tenaga ahli berpengalaman, siap memberikan pelayanan terbaik untuk kamu yang membutuhkan pemeriksaan Ferritin di kota surabaya,sidoarjo,gresik,jember,banyuwangi,solo,yogyakarta,bandung,bekasi,jakarta,tangerang,makassar,malang , apa itu Ferritin?, Kita ikuti penjelasannya dibawah. FERRITIN ADALAH Ferritin adalah sebuah protein yang terbisa di dalam sel pada hampir seluruh makhluk hidup. Ferritin merupakan protein penyimpan zat…. ...

Parahita Diagnostic Centre, Berpangalaman belasan tahun dengan ratusan tenaga ahli berpengalaman, siap menghasilkan pelayanan terbaik untuk kamu yang membutuhkan pemeriksaan Ferritin di kota surabaya,sidoarjo,gresik,jember,banyuwangi,solo,yogyakarta,bandung,bekasi,jakarta,tangerang,makassar,malang , apa itu Ferritin?, Kita ikuti penjelasannya dibawah. FERRITIN ADALAH Ferritin adalah sebuah protein yang terdapat di dalam sel pada hampir seluruh makhluk hidup. Ferritin merupakan protein penyimpan zat…. ...

Moderate lysosomal membrane permeabilization (LMP) is an important inducer of apoptosis. Macrophages are professional scavengers and are rich in hydrolytic enzymes and iron. In the present study, we found that LMP by lysosomotropic detergent MSDH resulted in early up-regulation of lysosomal cathepsins, oxidative stress and ferritin up-regulation, and cell death. Lysosomotropic base NH(4)Cl reduced the ferritin induction and oxidative stress in apoptotic cells induced by MSDH. Cysteine cathepsin inhibitors significantly protected cell death and oxidative stress, but had less effect on ferritin induction. We conclude that oxidative stress induced by lysosomal rupture causes ferritin induction with concomitant mitochondrial damage, which are the potential target for prevention of cellular oxidative stress and cell death induced by typical lysosomotropic substances in different disorders.. ...

Of the mothers, we classified 10 [5%] as having iron deficiency anaemia. The haemoglobin concentration and MCV were higher in the neonates than in their mothers but the MCHC was no different. Serum iron was much higher and total iron binding capacity much lower in the babies whose transferrin saturation was 72% compared with 14% for their mothers. There was a weak relationship between maternal and neonatal haemoglobin concentration and a direct relationship between fetal and maternal serum iron, but no relationship between maternal and fetal serum ferritin concentrations. It is suggested that it is the fetus which largely controls the movement of iron across the placenta with only a little control exerted by maternal serum iron level, and that cord ferritin is not a good indicator of fetal iron stores ...

Ferritin is the principal iron storage molecule found in animal cells. It is a globular protein complex about 10 nm in diameter composed of 24 subunits arranged as a hollow shell (apoferritin) in which iron atoms can be packed. A typical ferritin molecule contains about 2000 iron atoms at its core, but potentially may hold up to 4500. Pores are present on the surface of the complex allowing iron atoms to enter and be released from the core. In this manner ferritin is able to regulate levels of intracellular iron ...

TY - JOUR. T1 - Validity of plasma collection cards for ferritin assessment-A proof-of-concept study. AU - Koehler, Karsten. AU - Marks-Nelson, Eileen. AU - Braga, Camila P.. AU - Beckford, Safiya. AU - Adamec, Jiri. PY - 2020/6/1. Y1 - 2020/6/1. N2 - Objectives: Iron depletion is common around the world and among certain risk groups in developed countries. The overall purpose was to test the suitability of a novel plasma collection card for minimally invasive iron status assessment. Methods: Twenty participants (10 f/10 m) participated in this cross-sectional study. Ferritin and hemoglobin were measured from blood collected from a forearm vein, serving as reference method. Blood was also collected from the fingertip using the NoviplexTM Plasma Prep Card as well as capillary collection tubes. Results: There was substantial concordance between ferritin measured from samples collected via NoviplexTM and venous ferritin (concordance correlation coefficient (CCC) = 0.96) with a mean bias of −0.8 ...

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...

To diagnose a medical condition. Your doctor may suggest a ferritin test if other blood tests have shown that the level of oxygen-carrying protein in your red blood cells (hemoglobin) is low, or if the proportion of red blood cells to the fluid component in your blood (hematocrit) is low. These may indicate that you have iron deficiency anemia. A ferritin test can help confirm that diagnosis. Ferritin may also be measured in someone with restless legs syndrome.. A ferritin test may also be used to help diagnose conditions such as hemochromatosis, liver disease and adult Stills disease, among others.. When used to diagnose a medical condition, a ferritin test may be done in conjunction with an iron test and a total iron-binding capacity (TIBC) and transferrin test. These tests provide additional information about how much iron is in your body.. ...

Background and Aim: Women who are homozygous for the p.C282Y mutation in the HFE gene are at much lower risk of iron overload-related disease than p.C282Y homozygous men, presumably because of the iron-depleting effects of menstruation and pregnancy. We used data from a population cohort study to model the impact of menstruation cessation at menopause on serum ferritin (SF) levels in female p.C282Y homozygotes, with p.C282Y/p.H63D simple or compound heterozygotes and those with neither p.C282Y nor p.H63D mutations (HFE wild types) as comparison groups. Methods: A sample of the Melbourne Collaborative Cohort Study was selected for the HealthIron study (n = 1438) including all HFE p.C282Y homozygotes plus a random sample stratified by HFE-genotype (p.C282Y and p.H63D). The relationship between the natural logarithm of SF and time since menopause was examined using linear mixed models incorporating spline smoothing. Results: For p.C282Y homozygotes, SF increased by a factor of 3.6 (95% CI (1.8, ...

Iron deficiency is a common problem among frequent blood donors. The Canadian Blood Services recently sought to identify donors at highest risk of iron deficiency and the feasibility of large-scale ferritin testing. Over 1.5 years, ferritin testing was performed on 12,595 blood donors (2.6% of all donors) who had passed the hemoglobin screen. Donors with low ferritin levels (,25 µg/L) were informed of their low iron stores and advised to see their health care providers to ensure iron levels returned to normal. They were also advised not to donate blood for at least 6 months. Iron deficiency (,25 µg/L ferritin) was identified in 54% of female donors and 33% of male donors. Furthermore, over 41% of repeat male donors and 65% of repeat female donors had low iron levels. Almost 60% of donors deferred for low iron returned to donate within a year, but they donated less often and had a lower return rate compared to donors with normal iron levels. Since female donors are more prone to have low iron ...

Iron, together with thyroid, is probably one of the things most improperly tested for. With thyroid, doctors only measure TSH, which gives you almost no clue of actual thyroid function. Similarly with iron, doctors mainly test ferritin and then prescribe iron supplementation or phlebotomy based on just one marker. Pretty dangerous if you ask me.. A quick story. There was a man with significantly elevated ferritin, yet he was anemic. The doctor prescribed phlebotomy for years and the patient just got worse and his ferritin barely dropped. After a couple of years he had to quit his job and was administrated to a psychiatric hospital. After more than a decade they did some more tests and concluded that he was very low in copper and ceruloplasmin, which is needed to mobilize iron (ferritin) from the tissue for utilization in the body. For many years the doctors mainly tested ferritin and prescribed phlebotomy, whereas his problem was not mobilizing the iron and thus he got severe iron buildup ...

1. Mumbauer S, Pascual J, Kolotuev I, Hamaratoglu F (2019) Ferritin heavy chain protects the developing wing from reactive oxygen species and ferroptosis. PLoS Genet 15(9): e1008396. https://doi.org/10.1371/journal.pgen.1008396 31568497. ...

The functional roles of ferritin H and L subunits in ferrous iron oxidation and ferric iron hydrolytic polymerization were studied by combination of electrode oximetry and pH stat utilizing horse spleen and recombinant human H and L ferritins. Previous work has investigated ferrous ion oxidation kinetics, but no information on ferric core formation and its relationship to ferrous ion oxidation had been obtained. The kinetic pattern of iron oxidation/hydrolysis in H-chain homopolymer ferritin showed that the rate of iron hydrolysis is regulated by the rate of iron oxidation, and that both follow the iron ferroxidation pathway. In the early stage of the reaction with less than 10 Fe(II)/protein, it was observed that the stoichiometric ratio of proton release to ferrous iron consumption approaches zero, the ferrous iron to dioxygen ratio equals two and the kinetic ratio of proton release to dioxygen consumption approaches one. These results enable one to write a detailed mechanism for iron ferroxidation

Ferritin is a protein that cells make in order to sequester and store iron.. Free iron, that is, iron that is unattached to any other chemical moiety, can catalyze dangerous chemical reactions that damage cell structures. The body therefore strives to keep free iron under control by attaching it to ferritin. While the correlation between excess body stores of iron and ferritin levels is not perfect, in most cases the result is close.. The major storage site of iron in the human body is not ferritin, however, it is hemoglobin, which is the oxygen-transporting molecule of red blood cells. Around 80% or so of total body iron is found in hemoglobin.. The difference between hemoglobin and ferritin when it comes to iron storage is that hemoglobin levels are, in most cases, kept within a narrow range. Exceptions: in anemia, not enough hemoglobin is made, iron deficiency anemia being the most common form. In polycythemia, too many red cells are made and thus theres too much hemoglobin. Therefore, for ...

Iron status, as measured by blood counts and indices, serum iron, transferrin saturation, and serum ferritin values, was studied longitudinally in 56 infants on prolonged breast feeding, and compared to that of 29 infants receiving cow milk formula prepared at home and of 47 infants receiving a proprietary infant formula. The first two groups received no iron supplementation, whereas the proprietary formula was supplemented with iron. Although breast feeding was found to be sufficient to meet iron needs during the first 6 months of life, supplemental iron would be necessary during the second half of infancy in order to guarantee the optimal iron status. ...

There is evidence from the clinical literature of iron accumulation in the CNS of patients with ALS (Oba et al., 1993; Imon et al., 1995; Kasarskis et al., 1995). Although this has been known for some time, direct evidence of a role for iron in ALS has not been demonstrated thus far. Our data provide clear evidence that iron accumulation occurs in both neurons and glia but that different molecular mechanisms may contribute to iron accumulation in these two cell types. In glia, the increase in TfR1 that is likely to be mediated by the increase in IRP1 binding activity may underlie the influx of iron into these cells. Normally, IRP1 would be expected to concomitantly reduce the expression of ferritin (Pantopoulos, 2004). However, cytosolic ferritin expression goes up mainly in microglia, which could acquire iron from phagocytosis of dead and dying cells. Cytosolic ferritin mRNA expression is also known to be increased by proinflammatory cytokines or nitric oxide (Torti et al., 1988; Wei et al., ...

Anemia in older adults is a risk factor for numerous negative outcomes. There is no standard definition, but in most studies, anemia is defined as a hemoglobin value ,12 g/dL for women and ,13 g/dL for men. Absolute iron deficiency anemia is defined as the combination of anemia and the absence of total body iron. Serum ferritin is the most frequently used diagnostic parameter, but its concentration increases with age and in the presence of inflammatory diseases. Other laboratory tests, such as transferrin saturation, soluble transferrin receptor and the soluble transferrin receptor/ferritin index might provide useful information, but there is a wide variety in the cut-off values and interpretation of the results ...

Participants whose serum ferritin level was greater than 62 ng/ml had significantly higher odds of a glaucoma diagnosis when compared to those with a level less than 30 ng/ml, after adjustment for potential confounders (odds ratio [OR] 1.19, 95% confidence interval [CI] 0.806-1.758 for levels 31-61 ng/ml; OR 1.608, 95% CI 1.115-2.319 for levels 62-112 ng/ml; OR 1.892, 95% CI 1.279-2.800 for levels 113-3018 ng/ml).. ...

Islets of Langerhans were isolated from mouse pancreases and fixed in periodatelysine-paraformaldehyde. The fixed islets were then dissociated with trypsin and EDTA to yield cell suspensions that contained mainly four cell types; beta-cells, capillary endothelial cells, acinar cells, and pancreatic duct epithelial cells. The nonislet cells were probably associated wtih the surface of the isolated islets. The H-2 antigens of the dissociated pancreatic cells were labeled with an immunoferritin technique. Pancreatic duct epithelial cells showed specific ferritin labeling on their lateral cell membranes but not on apical microvillus membranes. Acinar cells were also labeled on lateral membranes, and the capillary endothelial cells were labeled on both the luminal and albuminal aspects of their surface membranes. In contrast, pancreatic beta-cells were unlabeled. The number of ferritin molecules per unit length of beta-cell membrane was essentially the same on cells from the antigenic strain and the ...

Medicine Journal in MJB authors are : Mahdi Yakoob Kzar,Ali Mihsen Hussein Salivary Iron And Ferritin Levels, Orofacial Complications of Patients With Thalassemia Major In Babylon Teaching Hospital For Maternity and Children university of babylon journals in the repository for farther content please log to http://repository.uobabylon.edu.iq

LIVER IRON CONTENT (LIC) IN ADULTS WITH NON-TRANSFUSION DEPENDENT SICKLE CELL DISEASE (NT-SCD). CORRELATION WITH SERUM FERRITIN AND LIVER ENZYMES CONCENTRATIONS

TY - JOUR. T1 - The Langerhans cell granule is an adsorptive endocytic organelle. AU - Takigawa, M.. AU - Iwatsuki, K.. AU - Yamada, M.. AU - Okamoto, H.. AU - Imamura, S.. PY - 1985. Y1 - 1985. N2 - The role of Langerhans cell granules (LCG) in adsorptive endocytosis was studied by using concanavalin A (Con A) and heterologous anti-epidermal cell serum (AES) as ligands. Isolated epidermal cells were obtained by trypsinization of guinea pig skin fragments and exposed to ferritin-conjugated Con A or AES followed by ferritin-conjugated second antibody at 37°C for various times up to 30 min. Langerhans cells among the treated epidermal cells were observed by electron microscopy. Shortly after the incubation, a few LCG in the cell periphery were filled with ferritin particles in the bulb portion. After prolonged incubation, ferritin-containing LCG increased in number and seemed to move to the juxtanuclear area. The granules were derived from plasma membrane invaginations that collected ferritin ...

The concentration of Soluble Transferrin Receptor (sTfR) is an indicator of iron status. Iron deficiency causes overexpression of transferrin receptor and sTfR levels, while iron repletion results in decreased sTfR levels. sTfR levels should be used to evaluate suspected iron deficiency in patients who may have inflammation, infection or chronic disease and other conditions in which ferritin concentration does not correlate with iron status. ...

Haemochromatosis treatment is straightforward. It involves taking blood a pint at a time to deplete the bodys iron stores in a procedure called venesection. A typical schedule could involve taking a pint of blood each week until serum ferritin levels are at the lower end of the normal range.

Order a Soluble Transferrin Receptor Blood Test to measure sTfR to diagnose iron deficiency in individuals with chronic disease, many of whom are Anemic.

Aalto University. Scientists from Aalto University, Finland, have succeeded in organizing virus particles, protein cages and nanoparticles into crystalline materials. These nanomaterials studied by the Finnish research group are important for applications in sensing, optics, electronics and drug delivery.. Layer structures, or superlattices, of crystalline nanoparticles have been extensively studied in recent years. The research develops hierarchically structured nanomaterials with tuneable optical, magnetic, electronic and catalytic properties.. Such biohybrid superlattices of nanoparticles and proteins would allow the best features of both particle types to be combined. They would comprise the versatility of synthetic nanoparticles and the highly controlled assembly properties of biomolecules.. The research group also discovered magnetic self-assemblies of ferritin protein cages and gold nanoparticles. These magnetic assemblies can modulate efficiently spin-spin relaxation times of surrounding ...

TY - JOUR. T1 - Body iron status and gastric cancer risk in the EURGAST study. AU - Fonseca-Nunes, A.. AU - Agudo, A.. AU - Aranda, N.. AU - Arija, V.. AU - Cross, A. J.. AU - Molina, E.. AU - Sanchez, M. J.. AU - Bueno-de-Mesquita, H. B.. AU - Siersema, P.. AU - Weiderpass, E.. AU - Krogh, V.. AU - Mattiello, A.. AU - Tumino, R.. AU - Saieva, C.. AU - Naccarati, A.. AU - Ohlsson, B.. AU - Sjoberg, K.. AU - Boutron-Ruault, M. C.. AU - Cadeau, C.. AU - Fagherazzi, G.. AU - Boeing, H.. AU - Steffen, A.. AU - Kuhn, T.. AU - Katzke, V.. AU - Tjonneland, A.. AU - Olsen, A.. AU - Khaw, K. T.. AU - Wareham, N.. AU - Key, T.. AU - Riboli, E.. AU - Peeters, P. H.. AU - Gavrila, D.. AU - Dorronsoro, M.. AU - Quiros, J. R.. AU - Barricarte, A.. AU - Jenab, M.. AU - Zamora-Ros, R.. AU - Freisling, H.. AU - Trichopoulou, A.. AU - Lagiou, P.. AU - Bamia, C.. AU - Jakszyn, P.. PY - 2015/10/9. Y1 - 2015/10/9. N2 - Although it appears biologically plausible for iron to be associated with gastric carcinogenesis, ...

The absorptive cell of the suckling rat ileum is specialized for the uptake and digestion of milk macromolecules from the intestinal lumen. The apical cytoplasm contains an extensive tubulocisternal system, a variety of vesicles and multivesicular bodies (MVB), and a giant phagolysosomal vacuole where digestion is completed. To determine if sorting of membrane-bound and fluid-phase macromolecules occurs in this elaborate endocytic system, we infused adsorptive and soluble tracers into ligated intestinal loops in vivo and examined their fates. Lysosomal compartments were identified by acid phosphatase histochemistry. Native ferritin and two ferritin-lectin conjugates that do not bind to ileal membranes (Con A, UEAI) served as soluble tracers. Horseradish peroxidase binds to ileal membranes and thus was not useful as a fluid-phase tracer in this system. Cationized ferritin and a lectin that binds to terminal B-D-galactosyl sites on ileal membranes (Ricinus communis agglutinin [RCAI]-ferritin) were ...

Nanoparticles have been widely used in biological and biomedical fields. To achieve certain purpose, nanoparticles should be functionalized with diverse molecules through genetic or chemical modification. Therefore, we developed genetically functionalized nanoparticles for improved systems by simple construction. In chapter 1, we developed genetically functionalized ferritin nanoparticles for immunoassay and imaging. Ferritins are known as self-assembled protein nanoparticles, and we fused human IgG-specific repebody to N-terminal heavy-chain ferritin subunit to construct repebody-ferritin nanoparticles. The repebody-ferritin nanoparticles were shown to have a three-order of magnitude higher binding affinity toward human IgG than free repebody due to multivalency. Also, the dye-labeled repebody-ferritin nanoparticles were applied for immunoassay such as western blot, cell imaging and flow cytometry. As a result, the repebody-ferritin nanoparticles generated much stronger fluorescent signals than ...