Identification of the iron-sulfur center of spinach ferredoxin-nitrite reductase as a tetranuclear center, and preliminary EPR studies of mechanism.

TY - JOUR. T1 - Enzymatic properties of the ferredoxin-dependent nitrite reductase from chlamydomonas reinhardtii. Evidence for hydroxylamine as a late intermediate in ammonia production. AU - Hirasawa, Masakazu. AU - Tripathy, Jatindra N.. AU - Sommer, Frederik. AU - Somasundaram, Ramasamy. AU - Chung, Jung Sung. AU - Nestander, Matthew. AU - Kruthiventi, Mahima. AU - Zabet-Moghaddam, Masoud. AU - Johnson, Michael K.. AU - Merchant, Sabeeha S.. AU - Allen, James Paul. AU - Knaff, David B.. PY - 2010/1. Y1 - 2010/1. N2 - The ferredoxin-dependent nitrite reductase from the green alga Chlamydomonas reinhardtii has been cloned, expressed in Escherichia coli as a His-tagged recombinant protein, and purified to homogeneity. The spectra, kinetic properties and substrate-binding parameters of the C. reinhardtii enzyme are quite similar to those of the ferredoxin-dependent spinach chloroplast nitrite reductase. Computer modeling, based on the published structure of spinach nitrite reductase, predicts ...

Antibodies for proteins involved in ferredoxin-nitrite reductase activity pathways, according to their Panther/Gene Ontology Classification

TY - JOUR. T1 - The role of tryptophan in the ferredoxin-dependent nitrite reductase of spinach. AU - Tripathy, Jatindra N.. AU - Hirasawa, Masakazu. AU - Kim, Sung Kun. AU - Setterdahl, Aaron T.. AU - Allen, James P.. AU - Knaff, David B.. PY - 2007/10/1. Y1 - 2007/10/1. N2 - A system has been developed for expressing a His-tagged form of the ferredoxin-dependent nitrite reductase of spinach in Escherichia coli. The catalytic and spectral properties of the His-tagged, recombinant enzyme are similar, but not identical, to those previously observed for nitrite reductase isolated directly from spinach leaf. A detailed comparison of the spectral, catalytic and fluorescence properties of nitrite reductase variants, in which each of the enzymes eight tryptophan residues has been replaced using site-directed mutagenesis by either aromatic or non-aromatic amino acids, has been used to examine possible roles for tryptophan residues in the reduction of nitrite to ammonia catalyzed by the enzyme.. AB - A ...

TY - JOUR. T1 - The role of tryptophan in the ferredoxin-dependent nitrite reductase of spinach. AU - Tripathy, Jatindra. AU - Hirasawa, Masakazu. AU - Kim, Sung-Kun. AU - Setterdahl, Aaron T.. AU - Allen, James P.. AU - Knaff, David. PY - 2007. Y1 - 2007. M3 - Article. SP - 1. EP - 12. JO - Photosynthesis Research. JF - Photosynthesis Research. SN - 0166-8595. ER - ...

Herbivore damage by chewing insects activates jasmonate (JA) signalling that can elicit systemic defense responses in rice. Few details are known, however, concerning the mechanism, whereby JA signalling modulates nutrient status in rice in response to herbivory. (15NH4)2SO4 labelling experiments, proteomic surveys, and RT‐qPCR analyses were used to identify the roles of JA signalling in nitrogen (N) uptake and allocation in rice plants. Exogenous applications of methyl jasmonate (MeJA) to rice seedlings led to significantly reduced N uptake in roots and reduced translocation of recently‐absorbed 15N from roots to leaves, likely occurring as a result of down‐regulation of glutamine synthetase cytosolic isozyme 1-2 and ferredoxin-nitrite reductase. Shoot MeJA treatment resulted in a remobilization of endogenous unlabelled 14N from leaves to roots, and root MeJA treatment also increased 14N accumulation in roots but did not affect 14N accumulation in leaves of rice. Additionally, proteomic and RT

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Plectonema boryanum bacteriophage LPP-2 ATCC ® 18200-B2™ Designation: LPP-2, strain SPI TypeStrain=False Application: Characterization

Stable isotope analysis of archaeological human remains is routinely applied to explore dietary habits and mobility patterns. The isotope information pertaining to the period prior to death may help in identifying locals and non-locals, especially when investigating individuals from the same funerary context but believed to have been highly mobile across the landscape. Based on the variety of the funerary goods in graves and what it is believed their diets comprised, it is thought that both local and non-local individuals were buried at the inland funerary site of Pica 8 (northern Chile, Late Intermediate Period, ∼1050-500 BP); however, uncertainties over the dietary intakes and mobility histories of these individuals still persist. The aim of this study is to refine the dietary characterization of a subset of Pica 8 individuals by increasing the temporal resolution of their dietary reconstructions, specifically throughout the last period of their life, and by identifying the multiple sources ...

A heptose is a monosaccharide with seven carbon atoms. They have either an aldehyde functional group in position 1 (aldoheptoses) or a ketone functional group in position 2 (ketoheptoses). There are few examples of C-7 sugars in nature, among which are: Sedoheptulose or D-altro-heptulose (a ketose), an early intermediate in lipid A biosynthesis Mannoheptulose, found in avocados L-glycero-D-manno-heptose (an aldose), a late intermediate in lipid A biosynthesis. Ketoheptoses have 4 chiral centers, whereas aldoheptoses have 5 ...

Genomics is the investigation of genomes, the entire arrangement of hereditary material inside a life form. Genomics includes the sequencing and examination of genomes. Genomics is additionally worried about the structure, capacity, examination, and advancement of genomes. As opposed to hereditary qualities, which alludes to the investigation of individual qualities and their parts in legacy, genomics utilizes high throughput DNA sequencing and bioinformatics to amass, and examine the capacity and structure of whole genomes.. ...

Ditto, graffiti. Evidence your nearest public restroom. Or the Lascaux caves. After all, cave paintings are graffiti by prehistoric yob, and the saucy rhyme next to your favorite American Standard is too! Both are simply reminders that we humans cant resist those clean white margins. We must share our wiles or wile away time.. I have mixed feelings about graffiti and marginalia. I suspect most people do.. I remember studying Don Quixote for the first time in high school. I was cross referencing several versions of the text including a crumbly old edition from the school library. I was frustrated with the marginalia. It was a library book after all! I was almost angry to think that a previous reader had felt obliged to foul the pristine pages. Almost, but not quite. I was also fascinated. I flipped through the book reading the notes.. I have a similar reaction to graffiti, annoyed when it feels like vandalism, fascinated when it feels like a subversive art form. During my freshman or sophomore ...

Thats Beachy Texturizing Surf Spray ($16) helps create sexy, free flowing waves for a loose tousled look. You can simply pop it in your beach bag and whip it out after emerging from the water or before heading out for the evening. To use: spray on dry or damp hair, shape with fingers or scrunch and youre done! The mist is lightly fragranced to mask that ocean or chlorine scent and it doubles as a decent leave-in. Since I have naturally curly hair I can only use the spray when my hair is flat ironed and dry so I usually wait until a couple days before wash day ...

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