Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. The HADHB gene is located on chromosome 1, with its specific location being 1p33-p32. The gene contains 5 exons. HADHB encodes a 51.2 kDa protein that is composed of 133 amino acids; 124 peptides have been observed through mass spectrometry data. Heart-type Fatty Acid-Binding Protein (H-FABP) is a small cytoplasmic protein (15 kDa) released from cardiac myocytes following an ischemic episode. Like the nine other distinct FABPs that have been identified, H-FABP is involved in active fatty acid metabolism where it transports fatty acids from the cell membrane to mitochondria for oxidation. See FABP3 for biochemical details. The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are ...
Introduction: Biomarkers would be helpful for early prognosis in out-of-hospital cardiac arrest (OHCA) patients. Heart-type fatty acid binding protein (H-FABP) is a tissue-specific protein which is rapidly released into the circulation when the cardiomyocyte injuries happen. The role of circulating H-FABP level for outcomes remains unclear in OHCA patients.. Hypothesis: The higher plasma level of H-FABP is associated with worse outcome in OHCA patients.. Methods: The study was a prospectively cohort study enrolling non-traumatic resuscitated OHCA patients in a tertiary medical center. All of the cardiac arrest event variables were recorded according to the Utstein style recommendations. Blood samples were collected at 24 hours after cardiac arrest and resuscitation. The primary endpoint was defined as the survival to discharge. The secondary endpoint was neurological status at time point of hospital discharge evaluated by cerebral performance category (CPC) score and survival to 90 ...
The association between ongoing myocardial damage and outcomes in patients who have received an implantable cardioverter-defibrillator (ICD) is unclear. Consecutive patients with cardiomyopathy, who had received an ICD (n = 107, mean age 65 ±
To the Editor We read with interest the systematic review and meta-analysis by Bruins Slot et al concerning early diagnosis of myocardial infarction (MI) using heart-type fatty acid-binding protein (H-FABP).1 We agree with their summary that when used in isolation, H-FABP may not offer a diagnostic advantage over the current troponin standard. However, it should be noted that in five of the included studies constituting 1573 patients (42% of the pooled cohort), no … ...
Li, H. [李慧颖]. (2010). Adipocyte fatty acid-binding protein : a link between inflammation and vascular dysfunction. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_ ...
Fatty acid binding protein 6, ileal (gastrotropin), also known as FABP6, is a protein which in humans is encoded by the FABP6 gene. This gene encodes the ileal fatty acid binding protein. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABP6 and FABP1 (the liver fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. Transcript variants generated by alternate transcription promoters and/or alternate splicing have been found for this gene. GRCh38: Ensembl release 89: ENSG00000170231 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000020405 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". "Entrez Gene: FABP6 fatty acid binding protein 6, ileal (gastrotropin)". Börchers T, Hohoff C, Buhlmann C, Spener F (1997). "Heart-type fatty acid binding protein - involvement in ...
This 10-year prospective study in 1069 patients with stable CHD at baseline provides the longest observational clinical evidence so far available for serum A-FABP and underlines that this circulating protein may be an important pathoyphysiological mediator of future cardiovascular morbidity and mortality in individuals with manifest atherosclerosis. In this population, increased serum A-FABP levels were also associated with metabolic and inflammatory cardiovascular risk factors at baseline, including adiposity, atherogenic dyslipidemia, hyperglycemia, hypertension, hs-CRP, and IL-6. During the long-term follow-up, A-FABP significantly predicted major secondary CVD events (cardiovascular death, nonfatal MI, or ischemic stroke) in crude analyses and for the most impactful outcome of cardiovascular death even after adjustment for established cardiovascular risk factors and lipid-lowering drugs. These findings suggest an independent proatherogenic role of this protein in serum, thereby expanding ...
1ICM: Escherichia coli-derived rat intestinal fatty acid binding protein with bound myristate at 1.5 A resolution and I-FABPArg106Gln with bound oleate at 1.74 A resolution.
Results The primary outcome measure of death or readmission with myocardial infarction after a minimum follow-up period of 12 months (median 18 months) occurred in 96 of 955 patients (10.1%). The H-FABP concentration was an independent predictor of death or myocardial infarction, after multivariate adjustment. Patients with H-FABP concentrations ,6.48 μg/l had significantly increased risk of adverse events (adjusted hazard ratio: 2.62, 95% confidence interval: 1.30 to 5.28, p = 0.007). Among troponin-negative patients (which constituted 79.2% of the cohort), the aforementioned cutoff of 6.48 μg/l identified patients at very high risk for adverse outcomes independent of patient age and serum creatinine. ...
TY - JOUR. T1 - Dynamics of internal water in fatty acid binding protein. T2 - Computer simulations and comparison with experiments. AU - Likić, Vladimir A.. AU - Prendergast, Franklyn G.. PY - 2001/4/1. Y1 - 2001/4/1. N2 - Multiple molecular dynamics (MD) simulations of fully solvated rat intestinal fatty acid binding protein (I-FABP) were conducted to investigate the dynamics of internal water molecules. Although the long time average of the number of internal water molecules in I-FABP is 22 as shown by the X-ray crystal structure, MD simulations predict large variations in the instantaneous number of internal water molecules on the nanosecond time scale. The computational model employed predicts that w135 (internal) and w217 (located on the protein surface) may be the water molecules with long residence times observed in previously reported magnetic relaxation dispersion studies. The average residence time of ∼20 internal water molecules occupying the fatty acid binding cavity is estimated ...
Alias: Fabph1, Fabph-1, Fabph4, Fabph-4, Fatty acid-binding protein, heart, Fatty acid-binding protein 3, Heart-type fatty acid-binding protein, H-FABP, Mammary-derived growth inhibitor, Mdgi, MDGI ...
TY - GEN. T1 - Heart fatty-acid binding protein gene variants are associated with intramuscular fat content and back fat thickness in pigs. AU - Gerbens, F.. AU - van Erp, A.J.M.. AU - Meuwissen, T.H.E.. AU - Veerkamp, J.H.. AU - te Pas, M.F.W.. PY - 1998. Y1 - 1998. M3 - Conference paper. SP - 187. EP - 190. BT - Proc. 6th world congress on genetics applied to livestock production 26, 1998. ER - ...
SummaryThe main objective was to evaluate the association between SNPs and haplotypes of the FABP1-4 genes and type 2 diabetes, as well as its interaction with fat intake, in one general Spanish population. The association was replicated in a second population in which HOMA index was also evaluated.Methods1217 unrelated individuals were selected from a population-based study [Hortega study: 605 women; mean age 54 y; 7.8% with type 2 diabetes]. The replication population included 805 subjects from Segovia, a neighboring region of Spain (446 females; mean age 52 y; 10.3% with type 2 diabetes). DM2 mellitus was defined in a similar way in both studies. Fifteen SNPs previously associated with metabolic traits or with potential influence in the gene expression within the FABP1-4 genes were genotyped with SNPlex and tested. Age, sex and BMI were used as covariates in the logistic regression model.ResultsOne polymorphism (rs2197076) and two haplotypes of the FABP-1 showed a strong association with the ...
F0019-75W1 -500ul : Fatty Acid Binding Protein, Heart (Heart-type Fatty Acid-binding Protein, H-FABP, Fatty Acid Binding Protein 3, FABP3, FABP11, Mammary-derived Growth Inhibitor, MDGI, Muscle Fatty Acid Binding Protein, M-FABP, O-FABP) (Biotin ...
Introduction: Ischemic stroke is associated with gastrointestinal complications such as constipation, dysphagia and fecal incontinence. However, the precise mechanisms involved have not been fully elucidated. D-Lactate and Intestinal Fatty-Acid Binding Protein (IFABP) are markers of intestinal mucosa integrity and barrier function. We evaluated serum concentrations of these markers in patients with acute ischemic stroke.. Materials and methods: We included consecutive patients with ischemic stroke, and used healthy, age and sex-matched subjects as controls. We excluded cases determined to be of other or undetermined etiology according to the TOAST classification. Blood was drawn within 24 hours of symptom onset. Serum concentrations of D-Lactate and IFABP were determined using commercially available colorimetric and ELISA kits, respectively.. Results: We included a total of 61 patients and 20 controls. The majority of patients were male (57.4%), and had a median age of 64 years. The most common ...
TY - JOUR. T1 - Serum concentrations of myoglobin vs human heart-type cytoplasmic fatty acid-binding protein in early detection of acute myocardial infarction. AU - Ishii, J.. AU - Wang, J.. AU - Naruse, H.. AU - Taga, S.. AU - Kinoshita, M.. AU - Kurokawa, H.. AU - Iwase, M.. AU - Kondo, T.. AU - Nomura, M.. AU - Nagamura, Y.. AU - Watanabe, Y.. AU - Hishida, H.. AU - Tanaka, T.. AU - Kawamura, K.. PY - 1997/8/19. Y1 - 1997/8/19. N2 - We compared the diagnostic utility of serum concentrations of human heart-type cytoplasmic fatty acid-binding protein (H-FABPc), myoglobin, and their ratio for the early diagnosis of acute myocardial infarction (AMI) in 104 healthy volunteers and 165 patients at admission within 6 h of the onset of chest pain. The ROC curves of the H-FABPc [0.946, 95% confidence interval (CI) = 0.913-0.979] and myoglobin (0.895, 95% CI = 0.8460.944) between patients with AMI and healthy volunteers were significantly greater than the area under the ratio of myoglobin to H-FABPc ...
The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016 ...
Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, BIOCHEMISTRY & MOLECULAR BIOLOGY, fatty acid-binding protein, FABP, liver, intestine, FLUORESCENT-PROBE ADIFAB, HEART, TRANSPORT, LIGAND, ADIPOCYTE, EXCHANGE, VESICLES ...
15 Jan 2013 New paper in Medical Decision Making by Mark Strong and Jeremy Oakley on fast efficient computation of partial EVPI. See publications page. 3 August 2012 Manuscript discussing the cost-effectiveness of presentation versus delayed troponin testing for acute myocardial infarction published in Heart. 29 May 2012 Registration still open for the Uncertainty in Computer Models 2012 Conference, July 2-4, 2012, Sheffield, UK. 16 May 2012 Manuscript discussing heart-type fatty acid binding protein as an early marker for myocardial infarction: systematic review and meta-analysis published online in Emerging Medicine Journal. 5 April 2012 Manuscript assessing the cost-effectiveness of presentation and delayed troponin testing for acute myocardial infarction submitted to Heart. 3 February 2012 Manuscript discussing the cost-effectiveness of cilostazol, naftidrofuryl oxalate, pentoxifylline and inositol nicotinate for the treatment of intermittent claudication in people with peripheral arterial ...
F0019-90C -50ug : FABP7, CT (Fatty Acid-binding Protein, Brain, Brain Lipid-binding Protein, BLBP, Brain-type Fatty Acid-binding Protein, B-FABP, Fatty Acid-binding Protein 7, Mammary-derived Growth Inhibitor Related, BLBP, FABPB, MRG ...
Cigarette smoking causes a decrease in the glomerular filtration rate in diabetic patients with normal renal function, independent of confounding factors including severe proteinuria (1). It increases the risk of microalbuminuria and accelerates the progression from microalbuminuria to overt proteinuria as well as the progression of renal failure in patients with type 2 diabetes (2). It is widely accepted that the rate of functional decline correlates with the degree of renal tubulointerstitial fibrosis. Previous studies have shown that renal function in patients with type 2 diabetes correlates better with tubular changes than with glomerular pathology (3). Further studies on tubulointerstitial injury in patients with diabetic nephropathy may provide additional insight into the pathogenesis of diabetic nephropathy and lead to the identification of therapeutic targets. Liver-type fatty acid-binding protein (l-FABP) is expressed in the proximal tubules, where it plays a key role in fatty acid ...
Peroxisome proliferator-activated receptors (PPARα, PPARβ, and PPARγ) are transcription factors activated by fatty acids and some antidiabetogenic drugs. Wolfrum et al. identified liver fatty acid binding protein (LFABP) as a protein that binds to the same ligands as does PPARα and that interacts directly with PPARα and PPARγ. They propose that LFABP, which can be found in both the cytoplasm and the nucleus, acts as a shuttle to deliver the PPAR agonists to the nuclear-localized PPAR receptors. In support of this model, transactivation of a PPAR reporter construct increased linearly with the amount of LFABP expressed.. C. Wolfrum, C. M. Borrmann, T. Börchers, F. Spener, Fatty acids and hypolipidemic drugs regulate peroxisome proliferator-activated receptors α- and γ-mediated gene expression via liver fatty acid binding protein: A signaling path to the nucleus. Proc. Natl. Acad. Sci. U.S.A. 98, 2323-2328 (2001). [Abstract] [Full Text]. ...
Science & Technology, Life Sciences & Biomedicine, Cell Biology, CELL BIOLOGY, BODIPY, fatty acid-binding protein, FABP, fluorescence, microscopy, CELL-LINE CACO-2, SPECTRAL PROPERTIES, CRYSTAL-STRUCTURE, LIVER, TRAFFICKING, ENDOCYTOSIS, INTESTINE, TRANSPORT, MEMBRANES, PROBES ...
How is Fatty Acid Binding protein 2 abbreviated? FABP2 stands for Fatty Acid Binding protein 2. FABP2 is defined as Fatty Acid Binding protein 2 frequently.
Insulin resistance is a characteristic feature in FCHL.9 10 Since genetic variation in the FABP2 gene could be the cause of insulin resistance, we screened for variants in this gene in patients with FCHL. According to our study, the FABP2 gene is not likely to be a major gene for FCHL. However, the polymorphism in codon 54 of this gene (Ala→Thr) may contribute to abnormalities in lipid metabolism in FCHL, although it does not have a significant effect on insulin resistance.. Complex segregation analysis has suggested a major gene effect on apoB28 and on triglycerides29 in FCHL. Although some uncommon variants in the lipoprotein lipase gene26 could cause FCHL, no gene that could explain a major part of this disease has yet been found. We found one novel nucleotide substitution in the 3′ noncoding region (A→G) of the FABP2 gene in patients with FCHL. In addition, we found nucleotide substitutions in codon 54 (GCT→ACT) and in codon 118 (GTA→GTG) described previously in Pima Indians.13 ...
0011] FIG. 1. (A) Schematic of the transgene vector (10871 bp) with porcine CFTR cDNA (1790-6238) driven by the rat intestinal fatty acid binding protein (iFABP) promoter (209-1419), flanked by the intervening sequence and the bovine growth hormone poly-A (BGHpA) (6295-6508), and followed by a hygromycin cDNA sequence (7392-8415) flanked by SV40 (7049-7373) and SV40 poly-A (8428-8800) signal sequences. Bgl I restriction site is denoted. (B) Gross image of a meconium plug (green-black colored portion of stool) that was passed by a CFTR-/-; Tg-FABP-pCFTR pig (line 1e) following an enema at approximately 18 h after birth. After the meconium plug was passed, a transition to normal-appearing stool was observed (yellow-green stool denoted by white arrows) bar=2 em. (C) Gross images from the gastrointestinal tract of CFTR-/-; Tg-FABP-pCFTR piglets. At birth, in 2 of the 5 lines meconium ileus was alleviated in CFTR-/-; Tg-FABP-pCFTR pigs. Meconium ileus lesions ranged from absent (left panel, line 1a) ...
250 µCi quantities of [1-14C]-Oleic Acid are available for your research. Application of [14C]Oleic Acid can be found in: trans-10, cis-12 conjugated linoleic acid decreasing de novo lipid synthesis in human adipocytes, effect of dietary phosphatidylcholine on the assimilation and distribution of ingested free oleic acid in aquaculture, variation of liver-type fatty acid binding protein content in the human HepG2 affecting the rate of fatty acid uptake, oleic acid stimulating rapid translocation of cholinephosphate cytidylyltransferase in type II cells, intestinal-fatty acid binding protein (I-FABP) and lipid transport in human intestinal epithelial cells, etc. ...
Mice null for adipocyte fatty acid binding protein (AFABP) compensate by increasing expression of keratinocyte fatty acid binding protein (KFABP) (Hotamisligil et al. Science 274:1377-1379, 1996). In the present study, AFABP knockout (KO) and wild-type (WT) mice became equally obese on a high-fat diet, as judged by fat pad weights, adipocyte size, and body composition analysis. High-fat feeding led to moderate insulin resistance in both WT and AFABP knockout mice, as indicated by an approximately 2-fold increase in plasma insulin. However, in the high fat-fed mice, plasma glucose levels were approximately 15% lower in the AFABP-KO mice. Adipocytes isolated from AFABP-KO and WT mice fed high- or low-fat diets exhibited similar rates of basal and norepinephrine-stimulated lipolysis and insulin-stimulated rates of glucose conversion to fatty acids and glyceride-glycerol. However, basal glucose conversion to fatty acids was higher in adipocytes of AFABP-KO mice. Adipocyte tumor necrosis factor-alpha ...
1AEL: Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein as monitored by 15N NMR relaxation and 1H exchange.
In this study, we examined the relationship between severity of CAD and heart-type fatty acid-binding protein (H-FABP), a new marker of ischemia in patients with non-ST-segment elevation acute ... Archives of the Turkish Society of Cardiology, Zeren, Gé° é»®, Erer, Hatice Beté»®, Ké½¬î §, Tuncay, 轪hin, Osman, Aksu, H黶eyin, Ké° r黮�, Diyar, G黺en�, ... ...
Self-association of the cardiac fatty acid-binding protein. Influence on membrane-bound, fatty-acid-dependent enzymes: Biochemistry
The Adipocyte Fatty Acid-Binding Protein (AFABP) is mainly expressed in fat cells. It can bind fatty acids and other lipophilic substances such as eicosanoids and retinoids. The peroxisome proliferator-activated receptor γ (PPARγ) is a nuclear receptor protein that requires ligand binding to regulate the specific gene transcription. PPARγ is expressed at extremely high levels in adipose tissue, macrophages, and the large intestine, where it controls lipid adipogenesis and energy conversion. Moreover, it has been found that AFABP and PPARγ can form a complex in vivo. It is proposed that AFABP carries the ligand and enters into the nucleus where it transfers the ligand to PPARγ by binding and macromolecular interaction. The goal of this project is to study the interaction between these two proteins in vitro, including their binding affinity, the location of the binding interface on the AFABP protein, and the dependence of these phenomena on specific ligands. New protocols were developed to obtain
Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid trafficking and metabolism. To better understand its tissue-specific regulation during zebrafish hepatogenesis, we isolated 5-flanking sequences of the zebrafish L-FABP gene and used a green fluorescent protein (GFP) transgenic strategy to generate liver-specific transgenic zebrafish. The 2.8-kb 5-flanking sequence of zebrafish L-FABP gene was sufficient to direct GFP expression in liver primordia, first observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42-51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is ...
Els macròfags desenvolupen un paper clau en la formació de la lesió dateroma. Les LDL oxidades (LDLox) indueixen lexpressió de la adipocyte fatty acid-binding protein (FABP4) en els macròfags, fet que constitueix un dels desencadenants de levolució daquestes cèl·lules al fenotip de cèl·lula escumosa. Les LDLox són una font daldehids, que són els productes finals de loxidació dels àcids grassos poliinsaturats. El treball realitzat ha estat dissenyat per provar la hipòtesi de la participació dels aldehids en la inducció de lexpressió de FABP4 en macròfags. A causa del caràcter prooxidant daquestes espècies, sha avaluat si aquesta inducció podria relacionar-se amb el sistema antioxidant cel·lular dirigit pel factor de transcripció Nrf2. El treball experimental es va dur a terme amb monòcits de la línia cel·lular THP-1 que es van diferenciar a macròfags mitjançant èsters de forbol. Els aldehids apolars estudiats van ser el 2,4-decadienal (DDE) i lhexanal. La ...
Preadipocyte factor-1 (Pref-1) is a transmembrane protein highly expressed in preadipocytes. Pref-1 expression is, however, completely abolished in adipocytes. The extracellular domain of Pref-1 undergoes two proteolytic cleavage events that generate 50 and 25 kDa soluble products. To understand the function of Pref-1, we generated transgenic mice that express the full ectodomain corresponding to the large cleavage product of Pref-1 fused to human immunoglobulin-γ constant region. Mice expressing the Pref-1/hFc transgene in adipose tissue, driven by the adipocyte fatty acid-binding protein (aP2, also known as aFABP) promoter, showed a substantial decrease in total fat pad weight. Moreover, adipose tissue from transgenic mice showed reduced expression of adipocyte markers and adipocyte-secreted factors, including leptin and adiponectin, whereas the preadipocyte marker Pref-1 was increased. Pref-1 transgenic mice with a substantial, but not complete, loss of adipose tissue exhibited ...
Alzheimers disease (AD) and vascular dementia (VaD) are intertwined by mixed dementia (MD) harboring varying degrees of AD pathology in combination with cerebrovascular disease. The aim was to assess whether there is a difference in the cerebrospinal fluid (CSF) profile, of selected proteins, between patients with VaD and MD with subcortical vascular disease (SVD), AD, and healthy controls that could contribute in the separation of the groups. The study included 30 controls, 26 SVD patients (9 VaD and 17 MD) and 30 AD patients. The protein panel included total tau (T-tau), hyperphosphorylated tau 181 (P-tau181), amyloid β 1-42 (Aβ1-42), neurofilament light (NF-L), myelin basic protein (MBP), heart fatty acid binding protein (H-FABP), matrix metalloproteinases (MMP-1, -2, -3, -9, and -10), and tissue inhibitors of metalloproteinases (TIMP-1 and -2). Immunochemical methods were utilized for quantification of the proteins in CSF and data analysis was performed with a multivariate discriminant ...
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The first step in determining whether a fluorescent dye can be used for antibody labeling consists in collecting data on its physical interaction with the latter. In the present study, the interaction between the 2-(2-hydroxy-5-nitrobenzylidene)-1,3-indanedione (HNBID) dye and the IgG1 monoclonal mouse antibody anti-human heart fatty acid binding protein (anti-hFABP) has been investigated by fluorescence and circular dichroism spectroscopies and complementary structural results were obtained by molecular modeling. We have determined the parameters characterizing this interaction, namely the quenching and binding constants, classes of binding sites, and excited state lifetimes, and we have predicted the localization of HNBID within the Fc region of anti-hFABP. The key glycosidic and amino acid residues in anti-hFABP interacting with HNBID have also been identified. A similar systematic study was undertaken for the well-known fluorescein isothiocyanate fluorophore, for comparison purposes. Our results
By immunohistochemistry, we have demonstrated that endothelial cells account for the immunoreactive band similar to E-FABP, previously detected in human heart, intestine, and adipose tissue by PAGE-immunoblotting analysis.7 8 By cloning and sequencing of an E-FABP cDNA from cultured human endothelial cells (HUVECs and DMVECs), we have demonstrated that endothelial E-FABP is identical to keratinocyte E-FABP.13 FABPs are generally abundant in tissues, ranging between 0.1% and 6% of total protein.1 2 3 We previously reported that E-FABP is an exception, since its levels, as measured by binding capacity, were low in normal human tissues compared with other tissue FABPs. Using PAGE-immunoblotting analysis, we have shown that E-FABP is expressed at relatively high levels, ranging from 0.5% to 2% of total protein in tissues, HUVECs, and DMVECs. The discrepancy between the values of these two measurements could be explained by posttranslational modifications of E-FABP, which lower the affinity for ...
Western-blot analysis using antiserum to 3T3-L1-cell fatty acid binding protein (FABP) revealed that pig adipose tissue contains a 15 kDa protein immunologically similar to the murine protein. This 15 kDa protein was purified from pig adipose tissue by sequential application of Sephadex G-50 gel filtration, cation exchange and covalent chromatography on Thiol-Sepharose-4B. The purity of the pig protein was established by two-dimensional polyacrylamide-gel electrophoresis. Isoelectric focusing indicated that the pig adipose FABP (a-FABP) exists with two charge isoforms (pI 5.1 and 5.2), both of which persist after delipidation. The N-terminus of the purified pig a-FABP was blocked; however, cleavage with CNBr allowed recovery of a 12-amino-acid peptide which was identical with the murine a-FABP sequence (residues 36-48) at 10 of 12 positions. The pig a-FABP bound 12-(9-anthroyloxy)oleic acid saturably and stoichiometrically, with an apparent dissociation constant of 1.0 microM. Northern-blot ...
How is Salicylic Acid-Binding Protein 3 abbreviated? SABP3 stands for Salicylic Acid-Binding Protein 3. SABP3 is defined as Salicylic Acid-Binding Protein 3 rarely.
A critical feature of obesity is enhanced insulin secretion from pancreatic β-cells, enabling the majority of individuals to maintain glycaemic control despite adiposity and insulin resistance. Surprisingly, the factors coordinating this adaptive β-cell response with adiposity have not been delineated. Here we show that fatty acid binding protein 4 (FABP4/aP2) is an adipokine released from adipocytes under obesogenic conditions, such as hypoxia, to augment insulin secretion. The insulinotropic action of FABP4 was identified using an invitro system that recapitulates adipocyte to β-cell endocrine signalling, with glucose-stimulated insulin secretion (GSIS) as a functional readout, coupled with quantitative proteomics. Exogenous FABP4 potentiated GSIS invitro and invivo, and circulating FABP4 levels correlated with GSIS in humans. Insulin inhibited FABP4 release from adipocytes invitro, in mice and in humans, consistent with feedback regulation. These data suggest that FABP4 and insulin form an
A critical feature of obesity is enhanced insulin secretion from pancreatic beta-cells, enabling the majority of individuals to maintain glycaemic control despite adiposity and insulin resistance. Surprisingly, the factors coordinating this adaptive beta-cell response with adiposity have not been delineated. Here we show that fatty acid binding protein 4 (FABP4/aP2) is an adipokine released from adipocytes under obesogenic conditions, such as hypoxia, to augment insulin secretion. The insulinotropic action of FABP4 was identified using an in vitro system that recapitulates adipocyte to beta-cell endocrine signalling, with glucose-stimulated insulin secretion (GSIS) as a functional readout, coupled with quantitative proteomics. Exogenous FABP4 potentiated GSIS in vitro and in vivo, and circulating FABP4 levels correlated with GSIS in humans. Insulin inhibited FABP4 release from adipocytes in vitro, in mice and in humans, consistent with feedback regulation. These data suggest that FABP4 and insulin form
We have analyzed two gene products expressed in the early endoderm of Xenopus laevis: Xlhbox-8, a pancreas-specific transcription factor and intestinal fatty acid binding protein (IFABP), a marker of small intestinal epithelium. Expression of the pancreas marker relies on cell signaling mediated by both the TGF-beta and FGF classes of secreted peptide growth factors, whereas, expression of the more posterior small intestinal marker does not. Endodermal explants devoid of mesoderm express both markers in a regionalized manner. Cortical rotation is required for the expression of the more anterior marker, Xlhbox-8, but not for the small intestinal marker, IFABP. These findings suggest that endodermal patterning is dependent, in part, on the same events and signals known to play important roles in mesodermal development. Furthermore, inhibition of TGF-beta signaling in the endoderm leads to ectopic expression of both mesodermal and ectodermal markers, suggesting the TGF-beta signaling may play a ...
Recombinant Human FABP4 is produced by our E.coli expression system and the target gene encoding Cys2-Ala132 is expressed with a 6His tag at the N-terminus. Bon Opus Cat. #C136
Ever since FABP5 was first identified as a novel keratinocyte protein highly upregulated in psoriatic skin (22), broad and diverse roles of FABP5 such as neurogenesis (25), protective effect from lipotoxic injury (20), carcinoma cell growth (12), mammary tumorigenesis (19), and keratinocyte differentiation (4) have been elucidated. Maeda et al. (24) found that deletion of FABP5 in mice resulted in reduction in body weight gain and improvement of insulin sensitivity under HFD. They emphasized the function of FABP5 in adipocytes on the grounds that adipocytes isolated from FABP5−/− mice exhibited enhanced insulin sensitivity and that mice with adipocyte-specific overexpression of FABP5 displayed increased systemic insulin resistance. However, the possibility that FABP5 expressed in other cell types is crucial for the phenotype of FABP5−/− mice cannot be excluded given the wide range of FABP5 expression in other tissues, including skin (31), lung (30.7), and brain (21).. Very recently, ...
Alterations of the small-intestinal permeability (s-IP) might play an essential role in both diarrhoea-predominant IBS (D-IBS) and celiac disease (CD) patients. Our aims were to analyse in D-IBS patients the symptom profile along with the levels of urinary sucrose (Su), lactulose (La), mannitol (Ma), and circulating biomarkers (zonulin, intestinal fatty acid binding protein - I-FABP, and diamine oxidase - DAO) of the gastrointestinal (GI) barrier function. The pro-inflammatory interleukins 6 and 8 (IL-6 and IL-8), the plasma values of lipopolysaccharide (LPS), and Toll-like receptor 4 (TLR-4) were also investigated. Besides, these biomarkers were compared with those in CD and healthy controls (HC). Finally, comparisons were performed between D-IBS patients with [D-IBS(+)] and without [D-IBS(−)] increased s-IP according to normal or altered La/Ma ratio. The study included 39 D-IBS patients, 32 CD patients, and 20 HC. GI permeability was assayed by high-performance liquid chromatography determination in
Inductions of FABP in hepatic cytosol by administration of tiadenol and clofibric acid were studied in rats, mice and guinea-pigs. In rats and mice, [1-14C]oleic acid-binding capacity of hepatic cytosol was increased, in association with induction of
article{2910d34e-79d3-4a7a-880b-f6f182695042, abstract = {Objective . Intestinal ischaemia is a life-threatening condition with high mortality, and the lack of accurate and readily available diagnostic methods often results in delay in diagnosis and treatment. The aim of this study was to investigate the accuracy of different plasma biomarkers in diagnosing intestinal ischaemia. Material and methods . Prospective inclusion of patients older than 50 years with acute abdomen admitted to hospital in Karlskrona, Sweden, between 2001 and 2003. Venous blood was sampled prior to any surgery and within 24 h from onset of pain. D-lactate, alpha glutathione S-transferase, intestinal fatty acid binding protein, creatine kinase B, isoenzymes of lactate dehydrogenase (LD) and alkaline liver phosphatase (ALP) were analysed. D-dimer was analysed using four different commercially available test kits. Results . In-hospital mortalities among patients with (n = 10) and without (n = 61) intestinal ischaemia were 40 ...
Carlsson M, Orho-Melander M, Hedenbro J, et al. (2000). "The T 54 allele of the intestinal fatty acid-binding protein 2 is associated with a parental history of stroke". J. Clin. Endocrinol. Metab. 85 (8): 2801-4. doi:10.1210/jc.85.8.2801. PMID 10946885 ...