Collagen triple helix repeat containing-1 (CTHRC1), which was firstly identified overexpressed in the adventitia and neointima of injured rat arteries, could inhibit collagen expression and increase cell migration. It was then found to be ubiquitously expressed in numerous cell types such as fibroblasts and smooth muscle cells, and aberrantly up-regulated in several malignant tumors. However, the functional role of CTHRC1 and its related mechanism in breast cancer still remains unclear. CTHRC1 expressions in breast cancer tissues and cells were assessed by qRT-PCR, western blot and immunohistochemistry. The relative expression level of miR-134, miR-155, miR-30c and miR-630 in breast cancer cells respectively was detected by qRT-PCR. Wild type (Wt) and Mutant type (Mut) CTHRC1 3UTR sequences were cloned into a psi-CHECK2 reporter vector, and the relative luciferase activity was detected by dual-luciferase reporter assay in indicated cells. The effect of ectopic expression of miR-30c or gain and loss of
CTHRC1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 222 aa (31-243a.a.) and having a molecular mass of 24.1kDa
Sigma-Aldrich offers abstracts and full-text articles by [Sunny A Abbah, Dilip Thomas, Shane Browne, Timothy OBrien, Abhay Pandit, Dimitrios I Zeugolis].
The extracellular matrix protein F-spondin mediates axon guidance during neuronal development. Its N-terminal domain, termed the reelin-N domain, is conserved in F-spondins, reelins, and other extracellular matrix proteins. In this study, a recombinant human reelin-N domain has been expressed, purified, and shown to bind heparin. The crystal structure of the reelin-N domain resolved to 2.0 A reveals a variant immunoglobulin-like fold and potential heparin-binding sites. Substantial conformational variations even in secondary structure are observed between the two chemically identical reelin-N domains in one crystallographic asymmetric unit. The variations may result from extensive, highly specific interactions across the interface of the two reelin-N domains. The calculated values of buried surface area and the interfaces shape complementarity are consistent with the formation of a weak dimer. The homophilic asymmetric dimer can potentially offer advantages in binding to ligands such as ...
Background. Persistent asthma is characterized by airway remodeling. Whereas we have previously shown that neither β(2)-agonists nor corticosteroids inhibit extracellular matrix (ECM) protein release from airway smooth muscle (ASM) cells, the effect
Background We discovered the gene Collagen Triple Helix Repeat Containing 1 (Cthrc1) and reported its developmental expression and induction in adventitial cells of injured arteries and dermal cells of skin wounds. The role of Cthrc1 in normal adult tissues has not yet been determined. Methodology/Principal Findings We generated mutant mice with a novel Cthrc1 null allele by homologues recombination. Cthrc1 null mice appeared developmentally normal. On the C57BL/6J background, livers from Cthrc1 null mice accumulated vast quantities of lipid, leading to extensive macrovesicular steatosis. Glycogen levels in skeletal muscle and liver of Cthrc1 null mice on the 129S6/SvEv background were significantly increased. However, Cthrc1 expression is not detectable in these tissues in wild-type mice, suggesting that the lipid and glycogen storage phenotype may be a secondary effect due to loss of Cthrc1 production at a distant site. To investigate potential hormonal functions of Cthrc1, tissues from adult mice
The present invention provides compositions that enhance or inhibit the interactions of galectin-8 and galectin-8-like proteins with other extracellular matrix proteins or cell surface receptors, and methods for the use thereof as physiological modulators of cell adhesion and in treatment of tumors, both in vivo or ex vivo. It further provides compositions and methods for modulating the expression of galectin-8, and galectin-8-like proteins, particularly to novel antisense oligonucleotides.
CTHRC1 antibody (collagen triple helix repeat containing 1) for ELISA, IHC-P, WB. Anti-CTHRC1 pAb (GTX31944) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
... , Authors: Yi-Hong Zhou. Published in: Atlas Genet Cytogenet Oncol Haematol.
MAAAPGLLVW LLVLRLPWRV PGQLDPSTGR RFSEHKLCAD DECSMLMYRG EALEDFTGPD CRFVNFKKGD PVYVYYKLAR GWPEVWAGSV GRTFGYFPKD LIQVVHEYTK EELQVPTDET DFVCFDGGRD DFHNYNVEEL LGFLELYNSA ATDSEKAVEK TLQDMEKNPE LSKEREPEPE PVEANSEESD SVFSENTEDL QEQFTTQKHH SHANSQANHA QGEQASFESF EEMLQDKLKV PESENNKTSN SSQVSNEQDK IDAYKLLKKE MTLDLKTKFG STADALVSDD ETTRLVTSLE DDFDEELDTE YYAVGKEDEE NQEDFDELPL LTFTDGEDMK TPAKSGVEKY PTDKEQNSNE EDKVQLTVPP GIKNDDKNIL TTWGDTIFSI VTGGEETRDT MDLESSSSEE EKEDDDDALV PDSKQGKPQS ATDYSDPDNV DDGLFIVDIP KTNNDKEVNA EHHIKGKGRG VQESKRGLVQ DKTELEDENQ EGMTVHSSVH SNNLNSMPAA EKGKDTLKSA YDDTENDLKG AAIHISKGML HEEKPGEQIL EGGSESESAQ KAAGNQMNDR KIQQESLGSA PLMGDDHPNA SRDSVEGDAL VNGAKLHTLS VEHQREELKE ELVLKTQNQP RFSSPDEIDL PRELEDEVPI LGRNLPWQQE RDVAATASKQ MSEKIRLSEG EAKEDSLDEE FFHHKAMQGT EVGQTDQTDS TGGPAFLSKV EEDDYPSEEL LEDENAINAK RSKEKNPGNQ GRQFDVNLQV PDRAVLGTIH PDPEIEESKQ ETSMILDSEK TSETAAKGVN TGGREPNTMV EKERPLADKK AQRPFERSDF SDSIKIQTPE LGEVFQNKDS DYLKNDNPEE HLKTSGLAGE PEGELSKEDH ENTEKYMGTE SQGSAAAEPE DDSFHWTPHT ...
Description of Clinical Activities: I work as an anatomic pathologist focusing on GI and liver pathology, and am also responsible for ocular pathology. I have a special interest in diagnostic molecular pathology. Description of Research Activities: My research focuses on investigating pathogenetic mechanisms of chronic diabetic complications Diabetic retinopathy and cardiomyopathy are two major areas of research in our laboratory. Current projects involve analyses of epigenetic mechanisms such as histone acetylation, microRNA alteration and their relationship with alterations of vasoactive factors and extracellular matrix protein production. We examine these mechanisms at multiple levels of complexity in an attempt to develop potential therapies using a wide variety of techniques.. ...
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While evidence is mounting that cells exploit protein unfolding for mechanochemical signal conversion (mechanotransduction), what mechanisms are in place to deal with the unwanted consequences of exposing hydrophobic residues upon force-induced prote
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The xCELLigence System from Roche Applied Science enables researchers to monitor and quantitatively assess cell attachment and spreading in real-time. The technique is non-invasive and does neither require lab- and cost-intensive cell labeling nor cell lysis or fixation. With this quick and economical method, the user can monitor the effect of matrix proteins on biological events in one single experiment.
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CTHRC1兔多克隆抗体(ab85739)可与小鼠, 人样本反应并经WB, IHC, ICC/IF实验严格验证并得到2个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
PURPOSE: To characterize the molecular defect in the TGFBI gene in a Chinese family affected with an atypical lattice corneal dystrophy.. DESIGN: Case report and experimental study.. METHODS: Molecular genetic analysis was performed on the DNA extracted from peripheral leucocytes from a Chinese family with atypical lattice corneal dystrophy. Fifty normal unrelated subjects of Chinese origin were used as controls. All exons of the TGFBI gene were amplified by polymerase chain reaction and directly sequenced.. RESULTS: Bilateral, symmetrical, ridgy round pattern of opacities with uneven surfaces and thin lattice lines were noted in the proband. Analysis of exon 14 revealed a heterozygous T to A transition on codon 625. The mutation was not detected in the unaffected family member and 50 unaffected individuals.. CONCLUSIONS: The novel TGFBI gene mutation (V625D) is associated with an early,onset variant of lattice corneal dystrophy. This case highlights the utility of molecular genetic analysis in ...
Expression of procollagen C-proteinase enhancer in cultured rat heart fibroblasts: evidence for co-regulation with type I collagen.: Procollagen processing by p
Corneal dystrophy is caused by mutations in the genes Corneal dystrophy is a hereditray disease caused by mutations in the genes coding for proteins in the cornea. So far, more than 60 different mutations are found in the gene encoding the protein Transforming Growth Factor Beta-Induced protein (TGFBIp), all of which result in a corneal clouding and thereby severe visual impairment and pain for the patient.. With the new research project, the researchers want to study the molecular mechanisms underlying the deposition of TGFBIp. The researchers will seek to determine the functions of the TGFBIp as a mean to better understand the pathology of corneal dystrophy.. Based on the biochemical studies, the researchers also plan to make relevant animal models, which are expected to contribute to a better understanding of the disease, and thereby ultimately to contribute to the development of a non-surgical treatment.. The project takes place at the Department of Molecular Biology and Genetics at Aarhus ...
We recently showed that differential expression of extracellular matrix (ECM) genes delineates four subgroups of breast carcinomas (ECM1, -2, -3- and -4) with different clinical outcome. To further investigate the characteristics of ECM signature and its impact on tumor progression, we conducted unsupervised clustering analyses in 6 additional independent datasets of invasive breast tumors from different platforms for a total of 643 samples. Use of four different clustering algorithms identified ECM3 tumors as an independent group in all datasets tested. ECM3 showed a homogeneous gene pattern, consisting of 58 genes encoding 43 structural ECM proteins. From 26 to 41% of the cases were ECM3-enriched, and analysis of datasets relevant to gene expression in neoplastic or corresponding stromal cells showed that both stromal and breast carcinoma cells can coordinately express ECM3 genes. In in vitro experiments, β-estradiol induced ECM3 gene production in ER-positive breast carcinoma cell lines, ...
|h6>Highlights|/h6> |ul> |li>miRNA expression is altered in cancer, often through aberrant methylation |/li> |li>Altered expression of miRNAs that regulate extracellular matrix gene expression is associated with development of metastatic cancer |/li> |li>Novel approaches exploiting miRNA technology will aid in development of new treatments and diagnostic tools|/li> |/ul>
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Extracellular Matrix Proteins (ECM Proteins) are macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur
Fibulin-2 is a protein that in humans is encoded by the FBLN2 gene. This gene encodes an extracellular matrix protein, which belongs to the fibulin family. This protein binds various extracellular ligands and calcium. It may play a role during organ development, in particular, during the differentiation of heart, skeletal and neuronal structures. Alternatively spliced transcript variants encoding different isoforms have been identified. FBLN2 has been shown to interact with Laminin, alpha 1, Laminin, alpha 5 and Perlecan. GRCh38: Ensembl release 89: ENSG00000163520 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000064080 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Zhang RZ, Pan TC, Zhang ZY, Mattei MG, Timpl R, Chu ML (January 1995). "Fibulin-2 (FBLN2): human cDNA sequence, mRNA expression, and mapping of the gene on human and mouse chromosomes". Genomics. 22 (2): 425-30. doi:10.1006/geno.1994.1404. PMID 7806230. "Entrez Gene: FBLN2 fibulin 2". Utani, A; ...
human EPYC protein: member of the small leucine-rich repeat proteoglycan (SLRP) family; predominantly expressed in cartilage; RefSeq NM_004950
Recently published in the Journal of Dermatological Science, the paper "Role of alpha5beta1 and MIA (melanoma inhibitory activity) in the pathogenesis of vitiligo" revealed to the world a new way to consider vitiligo.. The Italian research group led by Matteo Bordignon (MD, PhD) discovered the presence of a single protein called MIA (Melanoma Inhibitory Activity) as the possible real pathogenetic step for vitiligo development.. These findings open new possibilities to finally achieve a cure for 100 millions of worldwide spread patients suffering for this disfiguring disease.. ...
a cytonaute of molecular size traveling toward a cell, before reaching the plasma membrane, would first need to go through a jungle of stems, branches, rain forest vines, and lianas. In tissues, this messy tangle is the extracellular matrix. Extracellular matrix is a scaffold of proteins and carbohydrates located around the cells that is synthesized by the cells themselves. Some authors duggest that this definition only applies to the insoluble components of the extracellular matrix. Extracellular matrix was invented by multicellular organisms. It was needed to keep cells together by adhesion, and therefore tissues appeared. During evolution, extracellular matrix got many other functions, not just adhesion, such as being responsible for the mechanical properties of most tissues (both in plant and animals), keeping cell morphology, allowing cell communication, setting pathways for cell migration, modulating cell differentiation and physiology, keeping growth factors in some places, and many ...
All vascular implants, including stents, heart valves and graft materials exhibit suboptimal biocompatibility that significantly reduces their clinical efficacy. A range of biomolecules in the subendothelial space have been shown to play critical roles in local regulation of thrombosis, endothelial growth and smooth muscle cell proliferation, making these attractive candidates for modulation of vascular device biointegration. However, classically used biomaterial coatings, such as fibronectin and laminin, modulate only one of these components; enhancing endothelial cell attachment, but also activating platelets and triggering thrombosis. This review examines a subset of extracellular matrix molecules that have demonstrated multi-faceted vascular compatibility and accordingly are promising candidates to improve the biointegration of vascular biomaterials.
Complete information for TGFBI gene (Protein Coding), Transforming Growth Factor Beta Induced, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Synonyms for Extracellular matrix in Free Thesaurus. Antonyms for Extracellular matrix. 1 antonym for extracellular: intracellular. What are synonyms for Extracellular matrix?
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
The addition of the new sequences to our analysis has provided additional support that the olfactomedin and TIGR proteins are related throughout the length of the molecule rather than only in the olfactomedin domain in the C terminus. In Fig. 2, the GXCXXT motif and the leucine-rich/leucine-zipper regions of the sequences are highlighted. All of the olfactomedin- and TIGR-related sequences possess a residual leucine zipper aligned with the leucine zipper of TIGR. The additional sequences have also helped to adjust our previous alignments (1). When we align only the TIGR and olfactomedin sequences the N-terminal region includes a (A/V)LEE(E/Y)K motif spanning residues 151 through 156 in HTIGR and 135 through 140 in MOLFA. With the addition of the more divergent sequences, that region appears to be only partially conserved among the HTIGR, COLFA, and HOLFC proteins and is significantly more divergent among the other sequences.. In addition to the support from our sequence analysis, structural ...
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In the ten-year interval since the first edition of this volume went to press, our knowledge of extracellular matrix (ECM) function and structure has enor- mously increased. Extracellular matrix and c
View Notes - BIO 320 Lec18.2009.notes from BIO 50160 at University of Texas. Lecture 18 Extracellular Matrix: Interactions between Cells and Their Environment Figure 19-3 Molecular Biology of the
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CONTEXT AND OBJECTIVE: We evaluated the predictive value of serum cartilage oligomeric matrix protein (sCOMP) levels over 20 years on the development of radiographic (RKOA) and painful knee osteoarthritis (KOA) in a longitudinal cohort of middle-aged women. MATERIALS AND METHODS: Five hundred and ninety-three women with no baseline KOA underwent 5-year knee radiographs over 20-years and were asked about knee pain a month before each assessment. A repeated measures logistic regression model was used where the outcomes were recorded at 5, 10, 15 and 20-years follow-up. RESULTS: The highest quartile of sCOMP was associated with increased risk of RKOA with overall OR of 1.97 (95% CI: 1.33-2.91) over 20 years when compared with the lowest sCOMP quartile. The association with painful KOA was similar and also independent, but only when the fourth and third sCOMP quartiles were compared. DISCUSSION AND CONCLUSION: This study demonstrates that sCOMP levels are predictive of subsequent structural changes and
OBJECTIVE:,br /,To test the hypothesis that physiological cyclic loading during a 30-min walking exercise causes an increase in serum cartilage oligomeric matrix protein (COMP) concentration in a healthy population.,br /,METHODS:,br /,Blood samples (5 ml) were drawn from 10 physically active adults immediately before and after, and 0.5h, 1.5h, 3.5h and 5.5h after a 30-min walking exercise on a level outdoor walking track at self-selected normal speed. On a separate day, blood samples were drawn from the same 10 subjects during 6h while they were resting in a chair. Serum COMP concentrations were determined using a commercial enzyme-linked immunosorbent assay (COMP ELISA). An activity monitor was used to record basic time-distance measurements of gait. Serum COMP concentrations within the exercise protocol and within the resting protocol were compared using separate repeated measures analyses of variance (alpha=0.05).,br /,RESULTS:,br /,In the exercise protocol, a first increase (9.7%; P=0.003) ...
Chondrocytes and synovial cells synthesize Cartilage Oligomeric Matrix Protein (COMP) when activated by proinflammatory cytokines. The aim of this study was to analyze and compare ultrasound parameters of joint inflammation, effusion and synovitis with the levels of COMP in the serum of patients with primary osteoarthritis. Ultrasound was done and the concentration of COMP (ng/mL was examined in 88 patients. 75% of patients had effusion (size 10.13±4.35 mm), 62.5% had effusion in lateral recessus (LR), 28.4% (size 8.53±2.27 mm) in suprapatelar (SR), and 27.3% (size 11.38±4.44 mm) in medial (MR). 67% of patients had synovitis size 4.84±3.57 mm in SR, 3.15±1.86 mm in MR; and 6.09±2.80 mm in LR. 17.0% of patients had nodular type of synovitis, 30.7% had diffusive, and 19.3% nodular - diffusive. There was a significant link between the size of synovitis and effusion in SR (r=0.966, p=0.000), MR (r=0.812, p=0.009) and LR (r=0.886, p=0.003). The median of COMP concentration was 54 (44.5-58) ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
We report here that joint inflammation in collagen-induced arthritis is more aggravated in CD44-knockout mice than in WT mice, and we provide evidence for molecular redundancy as a causal factor. Furthermore, we show that under the inflammatory cascade, RHAMM (receptor for hyaluronan-mediated motility), a hyaluronan receptor distinct from CD44, compensates for the loss of CD44 in binding hyaluronic acid, supporting cell migration, up-regulating genes involved with inflammation (as assessed by microarrays containing 13,000 cDNA clones), and exacerbating collagen-induced arthritis. Interestingly, we further found that the compensation for loss of the CD44 gene does not occur because of enhanced expression of the redundant gene (RHAMM), but rather because the loss of CD44 allows increased accumulation of the hyaluronic acid substrate, with which both CD44 and RHAMM engage, thus enabling augmented signaling through RHAMM. This model enlightens several aspects of molecular redundancy, which is widely
Osteoarthritis Cartilage. 2011 Oct;19(10):1246-53. doi: 10.1016/j.joca.2011.07.011. Epub 2011 Jul 29. Research Support, Non-U.S. Govt
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As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
In the present investigation we show that CHAD, a relatively abundant noncollagenous protein in cartilage extracellular matrix, interacts with β1 integrins on bovine chondrocytes. This interesting finding identifies CHAD as a candidate for mediating signals between the chondrocytes and the cartilage matrix.. CHAD is a member of the LRR protein family (35). Among other members in this family are the small cartilage proteoglycans biglycan, decorin, fibromodulin, and lumican. These proteoglycans are all known to interact with collagen (18, 41, 47), but it is not known if CHAD interacts with collagen or other matrix molecules.. Chondrocyte adhesion to CHAD was partially inhibited by a rat polyclonal antibody against β1 integrin. Species differences between cells and antibodies may explain why the inhibition was not total. Alternatively, other receptors than β1 integrins may also be involved in the adhesion to CHAD. We found that adhesion of chondrocytes to CHAD was dependent on Mg2+ or Mn2+ but ...
OBJECTIVE: The authors goals were to establish the cellular origin of the reduced cortical reelin expression that occurs in schizophrenia and to relate it to markers of synaptic pathology. METHOD: In situ hybridization was used to quantify reelin mRNA in the hippocampal formation and dorsolateral prefrontal cortex of brains from 13 subjects with schizophrenia and 12 subjects without schizophrenia. Results were correlated with the expression of three synaptic protein genes in the dentate gyrus. RESULTS: Reelin mRNA was expressed by layer I neurons, interneurons, and interstitial white matter neurons. In subjects with schizophrenia, less reelin mRNA was expressed by interstitial white matter neurons in the hippocampal formation and by all three cell types in the prefrontal cortex. Reelin and synaptic protein expression correlated positively. CONCLUSIONS: Interstitial white matter neurons, presumed remnants of the cortical subplate, contribute to the reduction in reelin mRNA in schizophrenia. Down