TY - JOUR. T1 - DIURNAL CHANGES IN MATERNAL PLASMA ESTRONE AND ESTRADIOL AT 30 TO 31, 34 TO 35 AND 38 TO 39 WEEKS GESTATIONAL-AGE. AU - Challis, John. AU - Patrick, JE. AU - Campbell, K. AU - Natale, R. AU - Richardson, B. PY - 1980. Y1 - 1980. U2 - 10.1111/j.1471-0528.1980.tb04462.x. DO - 10.1111/j.1471-0528.1980.tb04462.x. M3 - Article. VL - 87. SP - 983. EP - 988. JO - BJOG: an International Journal of Obstetrics and Gynecology. JF - BJOG: an International Journal of Obstetrics and Gynecology. SN - 1470-0328. IS - 11. ER - ...
These results are consistent with previously published studies indicating an important role for CYP1A2 in the liver (31, 35, 36). Again, we observed higher velocities and lower apparent Km values than previously reported. The extent of the contribution by CYP2C19 to 2-hydroxylation of estrone has been overlooked in previous studies, either as a result of not examining the recombinant enzyme or as a result of comparing activities at high substrate concentrations.. Based on the results from the recombinant enzymes, one would predict that CYP1A2 would be the major contributor at low estrone concentrations to 4-hydroxylation of estrone, but that CYP3A4/5 (see Table 3) could contribute as estrone concentrations increased. Only the CYP2C9 marker activity correlated with 4-hydroxylation of 25 μmol/L estrone in human hepatic microsomes. However, the lack of correlation with CYP1A2 marker activities may reflect the contribution of multiple enzymes at this high substrate concentration, as furafylline and ...
TY - JOUR. T1 - Endocrine profile in a patient with familial breast cancer. A case-control study. AU - Lynch, Henry T.. AU - Harris, R. E.. AU - Fishman, J.. AU - Lynch, J. F.. AU - Marrero, K.. AU - Maloney, K.. PY - 1979. Y1 - 1979. N2 - Urinary and blood hormonal profiles were studied throughout a monthly cycle in a patient with familial breast cancer. Two comparison cohorts (one high-risk and one low-risk) were studied concurrently. Findings disclosed that our breast cancer-affected patient showed a distinctive hormonal pattern characterized by significant elevation throughout the cycle of plasma estrone, estradiol, and prolactin. Save for a depression in plasma FSH in the early follicular phase, this hormone, as well as LH and progesterone patterns in our patient, were similar to the comparison cohorts. Urinary estrone and estradiol patterns in our patient were elevated early in the follicular phase. Our patient also showed a depression in urinary estrone, estradiol, and estriol following ...
... ,The procedure follows the basic principle of a competitive enzyme immunoassay where there is competition between an unlabeled antigen and an labelled antigen bound to the limited binding sites of a specific antiserum . The amount of enzyme conjugate complex (HRP-avidin: biotin-labelled estrone) bou,medicine,medical supply,medical supplies,medical product
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Estrone, a synthetically prepared or naturally occurring steroidal estrogen obtained from pregnant equine urine, is the primary circulating estrogen after menopause. Estrone is naturally derived from the peripheral conversion of androstenedione by an aromatase enzyme found in adipose tissues and is converted to estradiol in peripheral tissues. Estropipate is piperazine-stabilized estrone sulfate. Estrone, and estropipate are used to treat abnormalities related to gonadotropin hormone dysfunction, vasomotor symptoms, atrophic vaginitis, and vulvar atrophy associated with menopause, and for the prevention of osteoporosis due to estrogen deficiency ...
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BioAssay record AID 682056 submitted by ChEMBL: TP_TRANSPORTER: uptake (pH 5.0) of Estrone-3-sulfate at a concentration of 5.43 nM in OATP-B-expressing HEK293 cells.
I have been on TRT now for about 3 months. Ive noticed very sore and puffy nipples. I actually can feel the swollen gland in my nipple. Ive been taking 200mg/week test, .25mg anastrozole EOD. I just had my labs done and my test was 1200 and E2 21. The only abnormal was my Estrone was 87 with top scale being 77. Does anyone have any idea why this is happening? I thought controlling my E2 was not going to let this happen. Should I take clomid to help rid the estrogen from my receptors? Im just ...
Hi guys, I recently went off a medication, and after a horrible time with withdrawls, Im back to normal. There is one MAJOR problem.... I
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In our continuing quest to design efficient inhibitors of estrone sulfatase activity and to assess the recognition of estrone sulfate surrogates by estrone sulfatase, we synthesized and evaluated several sulfonate derivatives of 5,6,7,8-tetrahydronaphth-2-ol and estrone. 5,6,7,8-Tetrahydronaphth-2-methanesulfonate (11), and 5,6,7,8-tetrahydronaphth-2-(p-toluene)sulfonate (12) were found not to inhibit estrone sulfatase activity; estrone-3-methane-sulfonate (5), estrone-3-ethanesulfonate (6), estrone-3-butanesulfonate (7), and estrone-3-[(+)10-camphor]sulfonate (8) all weakly inhibited estrone sulfatase, and the best inhibitor, from this class of compounds, was estrone-3-(p-toluene)sulfonate (9). At 10 microM, it inhibited estrone sulfatase activity by 91%. These results emphasize some of the requirements needed for high-affinity binding to the enzyme.
OBJECTIVES : The purpose of this study was to evaluate the effect of cigarette smoking and alcohol use on the risk of endometrial cancer. The impact of smoking on serum estrone, estradiol, and androstenedione levels also was examined. STUDY DESIGN : This hospital-based case-control study included 168 women with endometrial carcinoma and 334...
A series of novel D-ring modified derivatives of estrone was synthesized and tested as inhibitors of steroid sulfatase (STS). The steroidal D-ring was cleaved via an iodoform reaction and thermal condensation of the resulting marrianolic acid derivative gave 16,17-seco-estra-1,3,5(10)-triene-16,17-imide derivatives, where a piperidinedione moiety is in place of the D-ring. This synthetic approach was found to give a higher overall yield than the literature method of Beckmann rearrangement. A range of alkyl side chains have been introduced on the nitrogen atom of the imido-ring and the corresponding 3-O-sulfamates synthesized. The new D-ring modified estrone derivatives bearing a propyl (39) and a 1-pyridin-3-ylmethyl (46) moiety had IC(50) values of 1 nM when tested in placental microsomes for the inhibition of STS. These compounds are therefore up to 18-fold more potent than EMATE, the very first highly potent irreversible steroidal STS inhibitor.
Dr. Weeks Comment: High testosterone is considered by some as a risk for prostate cancer. Many of us have suspected high estrogen instead and the research is driving the attention here, to estrone, and away from testosterone. Have your estrone level tested if you are worried about prostate cancer. "…In this cohort of older men, higher estrone levels were strongly associated with an increased risk of incident prostate cancer…". Sex hormones and the risk of incident prostate cancer.. Urology. 2010 Nov;76(5):1034-40. Epub 2010 May 7.. Daniels NA, Nielson CM, Hoffman AR, Bauer DC; Osteoporotic Fractures In Men (MrOS) Study Group.. Abstract. OBJECTIVE: To assess whether sex hormone levels are associated with subsequent development of prostate cancer.. METHODS: A case-cohort study was conducted within the ongoing Osteoporotic Fractures in Men cohort study of community-dwelling men ≥ 65 years old recruited at 6 US clinical sites. After a mean follow-up of 4.7 years, all men with ...
Hormonin tablets contain three active ingredients: estradiol, estriol and estrone (previously spelt oestradiol, oestriol and oestrone in the UK). These are all naturally occuring forms of the main female sex hormone, oestrogen.
These data indicate that the hormonal effects due to moderate alcohol consumption are seen early, within 4 weeks of initiation of ingestion.
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Materials. [3H]Methotrexate (46.8 Ci/mmol) and [3H]folate (30 Ci/mmol) were obtained from Moravek Biochemicals (Brea, CA), [3H]cimetidine (25.0 Ci/mmol) was from GE Healthcare (Chalfont St. Giles, UK) and [3H]estrone sulfate (57.3 Ci/mmol) and [3H]taurocholate (1.2 Ci/mmol) were from PerkinElmer Life Sciences, Inc. (Boston, MA). Folate, methotrexate, 5-methyltetrahydrofolate, folinate, geneticin, and sulfasalazine were obtained from Sigma-Aldrich Co. (St. Louis, MO). Zinc protoporphyrin IX (Calbiochem, San Diego, CA) was obtained from Merck (Darmstadt, Germany). Restriction enzymes were obtained from TOYOBO (Tokyo, Japan). All other reagents were of analytical grade and commercially obtained.. Cell Culture. Human embryonic kidney (HEK)293 and MDCKII cells were maintained at 37°C and 5% CO2 in Dulbeccos modified Eagles medium with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin.. Isolation of hPCFT/HCP1. The cDNA of hPCFT/HCP1 was cloned from the human intestine total ...
advanced pms complex clinically proven nutrients for nutritional pms support - BioResponse DIM - Patented, enhanced bioavailability and clinically proven to help balance hormones - Chasteberry extract - Clinically shown to help reduce PMS symptoms - Natural PMS support herbs and select nutrients including Calcium Clinical research has shown that the ingredients in PMSHarmony, including BioResponse DIM, Calcium, Magnesium, Vitamin D and Chasteberry extract help decrease many of the symptoms associated with PMS and help balance hormones. BioResponse DIM is a naturally-occurring indole found in cruciferous vegetables. Clinical research has shown it helps support healthy estrogen metabolism by increasing production of good estrogen metabolites, such as 2-hydroxy estrone, while reducing harmful metabolites, such as 16 alpha-hydroxy estrone. Patented BioResponse DIM utilizes solubility-enhancing micro-encapsulation technology that encases small particles of Diindolylmethane within a
1793 Purpose: To evaluate the associations of genetic polymorphisms in estrogen synthesis and metabolism enzymes CYP19, 17β-HSD1, CYP1A1, and CYP1A2 genes with blood levels of sex hormones among postmenopausal Chinese women. Method: Included in this study were 420 postmenopausal community controls from a population-based case-control study conducted in Shanghai. Fasting blood samples from those women were measured for blood DHEA, estradiol, estrone, estrone-sulfate, SHBG and testosterone. Genetic polymorphisms in the CYP19 codon 264, CYP1A1, and CYP1A2 were genotyped using PCR-RFLP methods. The CYP19 3-bp deletion and 17β-HSD1 (TTTA)n repeat and 12-bp deletion in 5′-UTR were genotyped by detecting fluorescent PCR amplimers on an ABI3700 automated DNA sequencer. Differences in plasma levels of hormones by genotypes were examined using variance analysis. Results: The geometric means of plasma levels of DHEA, estradiol, estrone, estrone-sulfate, SHBG and testosterone are 627.09 ng/ml, 10.41 ...
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Dehydration of theophylline monohydrate powder-effects of particle size and sample weight. Int J Pharma 1994 106 33-40. Ahlneck C, Zografi G. The molecular basis of moisture effects on the physical and chemical stability of drugs in the solid state. Int J Pharm 1990 62 87-95. Ahmed S, Owen CP, James K, Patel CK, Patel M. Acid dissociation constant, a potential phys-icochemical factor in the inhibition of the enzyme estrone sulfatase ES . Bioorg Med Chem Lett 2001, 9 Apr 11.... ...
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2-Deoxy-2-fluoro-D-glucopyranosides derived from phenol, estrone and 1,2:3,4-di-O-isopropylidene-α-D-galactopyranose were prepared and the rates of acid hydrolysis of phenyl β-D-glucopyranoside and phenyl 2-deoxy-2-fluoro-β-D-glucopyranoside compared.. ...
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Estriol 16-a-glucuronide is intended for Pharmaceuticals applications. All information about [2H3]-Estriol 16-a-glucuronide is provided in the MSDS. We deliver compounds with high purity levels and a comprehensive Certificate of Analysis. Connect to your member account to consult the documents ...
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TY - JOUR. T1 - Circulatory responses to systemic infusions of estrone and estradiol-17α in nonpregnant, oophorectomized ewes. AU - Rosenfeld, Charles R.. AU - Rivera, Richard. PY - 1978/10/15. Y1 - 1978/10/15. N2 - Uterine arterial blood flow dose-response curves to systemic infusions of either estrone (E1) or estradiol-17α (E2α) were determined in ten oophorectomized, nonpregnant ewes. Maximum uterine blood flow occurred with 5 μg per kilogram of E1 and ≥ 20 μg per kilogram of E2α. Total uterine blood flow measured with isotope-labeled microspheres rose more than tenfold following 30 μg per kilogram of either estrogen, as did blood flow to the myometrium, endometrium, and uterine caruncles (p , 0.05). Distribution of uterine blood flow was unaltered. Cervical and vaginal blood flows increased ten-to twenty-fold with E1 and E2α, while mammary gland blood flow rose 54 and 77 per cent. Significant increases in adrenal and thyroid blood flows were documented. The only significant ...
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Dr. Joshua Gatson, Assistant Professor of Surgery at the University of Texas Southwestern Medical Center in Dallas, investigates biomarkers and novel therapies for traumatic brain injury. His previous work has shown that estrone, one of the three naturally occurring estrogen hormones in the body, has shown some promise in reducing inflammation and cell death in the brain. His latest study is the first to demonstrate estrone provides those anti-inflammatory and antioxidant capabilities after traumatic brain injury. It is likewise the first to reveal the cellular pathways that are involved. His findings will be presented April 22, 2012 during Experimental Biology 2012 in San Diego, CA.. The study, conducted in male rats, compared 0.5 mg of estrone to a placebo, both given 30 minutes after the injury. It demonstrated that estrone is involved in promoting brain-derived neurotrophic factor (BDNF), which promotes cell survival. "BDNF, one of the main growth factors that regulates repair following ...
Looking for Dehydroepiandrosterone sulfate? Find out information about Dehydroepiandrosterone sulfate. C19H28O2 Dimorphous crystals with a melting point of 140-141°C, or leaflet crystals with a melting point of 152-153°C; soluble in alcohol, benzene, and... Explanation of Dehydroepiandrosterone sulfate
Esterified estrogens contain a mixture of estrogenic substances; the principle component is estrone. Preparations contain 75% to 85% sodium estrone sulfate and 6% to 15% sodium equilin sulfate such that the total is not <90%. Esterified estrogens are a man-made mixture of estrogens that are used to treat symptoms of menopause such as hot flashes, vaginal dryness, vaginal burning or irritation, or other hormonal changes in the vagina. It is being also for the prevention and treatment of osteoporosis.
use.. Androstenedione offers a fast-acting, over-the-counter alternative to prescription-only testosterone patches, creams and lotions. It is sold in capsules or pills for oral use, and it has recently become available in a liquid spray for sublingual use. The sublingual spray raises testosterone levels in less than 30 minutes.. The problem with androstenedione is that it is quickly metabolized into estrogens. Estrogens have strong anti-testosterone-like metabolic effects that last for a long time (days as opposed to hours). Androstenedione produces estrogens through two separate pathways. First, it can be directly metabolized into estrone (see Figure 1). Second, it can be metabolized to testosterone and then to estradiol. Both estradiol and estrone are strong estrogens which suppress the anabolic, metabolism-enhancing, fat-loss and tissue-healing properties of testosterone. I believe that many people will suffer estrogen-related side effects from androstenedione that will have far more negative ...
Oestriol hormone. Molecular model of the structure of the female sex hormone oestriol. Atoms are represented as rods and are colour-coded: carbon (blue), hydrogen (gold), and oxygen (red). Oestriol is one of three naturally occurring oestrogens, a class of hormones that are associated with the development of the female reproductive organs and secondary sex characteristics. Oestriol is produced at high levels by the placenta during pregnancy, although it is secreted by the ovaries at low levels in non-pregnant women. If oestriol levels are abnormally low during pregnancy, this can indicate that there is a problem with the development of the foetus. - Stock Image A619/0147
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Specific Aim 1: To determine whether aromatase activity is measurable in adipose tissue using the compound (see appendix) as a precursor and whether aromatase is decreased in the adipose tissue of obese women, and whether this varies by regional fat location. The investigators will accomplish this by examining precursor/product ratios of hormones infused through microdialysis using the compound as a substrate.. Hypothesis for specific aim 1: Aromatase activity will be measurable in adipose tissue using testosterone as a precursor. Estradiol, but not estrone production from androgen precursors will be decreased in obese women relative to those of normal weight.. Specific Aim 2: To determine whether nonspecific, systemic suppression of inflammation will lead to improved reproductive hormonal profiles of luteinizing hormone (LH), follicle stimulating hormone(FSH), Estrone conjugates (E1c) and pregnandiol glucuronide (Pdg) in obese women who undergo two menstrual cycles of study: one off ...
A hydroxylated metabolite of estradiol or estrone that has a hydroxyl group at C3-beta, 16-alpha, and 17-beta position. Estriol is a major urinary estrogen. During pregnancy, large amount of estriol is produced by the placenta. Isomers with inversion of the hydroxyl group or groups are called epiestriol. Though estriol is used as part of the primarily North American phenomenon of bioidentical hormone replacement therapy, it is not approved for use by the FDA or Health Canada. It is however available in the United States by prescription filled only by compounding pharmacies. It has also been approved and marketed throughout Europe and Asia for approximately 40 years for the treatment of post-menopausal hot flashes.
Potent and highly selective aromatase (CYP19) inhibitor (IC 50 = 15nM) that has no discernible effect on adrenocorticoid hormone synthesis. Reduces plasma estrogen levels and exhibits antitumor activity in vivo . Orally active.
I am new to cell culture and no one in my dept has worked with hormones before so I am completly out of my element here. My question is what stock concentration people use when working with estrogen and progesterone? I need a final estrogen concentration of 10-8 and a final progesterone cencentration of 10-7. I have already tried making stocks and working solutions myslef, but the cells never expressed markers of differentiation which that are supposed to do under hormone treatment. So I did something wrong ...
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TY - JOUR. T1 - The Effects of Estrone (Ogen) on Spinal Bone Density of Postmenopausal Women. AU - Harris, Steven T.. AU - Genant, Harry K.. AU - Baylink, David J.. AU - Gallagher, J. Christopher. AU - Karp, Sherry Katz. AU - Mcconnell, Maureen A.. AU - Green, Elizabeth M.. AU - Stoll, Ralph W.. PY - 1991/10. Y1 - 1991/10. N2 - The effects of cyclical treatment with estrone sulfate (0.3, 0.625, or 1.25 mg), plus calcium carbonate, on spinal trabecular bone density were compared with placebo in 120 postmenopausal women in this 2-year, multicenter, doubleblind study. While the placebo and 0.3-mg treatment groups lost bone density (-3.6% and -5.1%), the 0.625- and 1.25-mg treatment groups experienced no significant change from baseline at 24 months (-0.8% and +0.7%). The 1.25-mg treatment group was significantly different from the placebo group at 12,18, and 24 months. Although the 0.625-mg treatment group was significantly different from the placebo group only at 18 months, the data suggest that ...
Accepted name: estrone sulfotransferase. Reaction: 3-phosphoadenylyl sulfate + estrone = adenosine 3,5-bisphosphate + estrone 3-sulfate. Glossary: 3-phosphoadenylyl sulfate = PAPS. Other names: 3-phosphoadenylyl sulfate-estrone 3-sulfotransferase; estrogen sulfotransferase; estrogen sulphotransferase; oestrogen sulphotransferase; 3-phosphoadenylylsulfate:oestrone sulfotransferase. Systematic name: 3-phosphoadenylyl-sulfate:estrone 3-sulfotransferase. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9026-06-6. References:. 1. Adams, J.B. and Poulos, A. Enzymic synthesis of steroid sulphates. 3. Isolation and properties of estrogen sulphotransferase of bovine adrenal glands. Biochim. Biophys. Acta 146 (1967) 493-508. [PMID: 4965224]. 2. Rozhin, J., Zemlicka, J. and Brooks, S.C. Studies on bovine adrenal estrogen sulfotransferase. Inhibition and possible involvement of adenine-estrogen stacking. J. Biol. Chem. 252 (1967) 7214-7220.. 3. Adams, J.B., Ellyard, ...
Preadipocytes (3T3 L1) were used between 7 and 14 days after differentiation; they were incubated with 44 nM 3H-esterone. The medium was supplemented with 1 μM recombinant murine leptin, 10 nM recombinant human insulin, or 1 μM corticosterone for up to 72 hr. In a second series of experiments, cells were incubated for 48 hr with different concentrations of leptin, insulin or corticosterone, and compared with controls (plain medium). Cells were harvested, washed in buffer and homogenized, and protein was measured. Lipid extracts of cell homogenates were used for HPLC; the label distribution in free and acyl-estrone peaks was measured. Overall uptake of estrone (i.e., the sum of free and acyl-estrone) by cells was not affected by leptin or corticosterone, but strongly reduced by insulin. Leptin and corticosterone increased the synthesis of acyl-esterone in a dose- and time-dependent way. Insulin decreased acyl-estrone synthesis at low concentrations and with little change over time. The results ...