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TY - JOUR. T1 - Plasma erythromycin concentrations predict feeding outcomes in critically ill patients with feed intolerance. AU - Nguyen, N. Q.. AU - Grgurinovich, N. G.. AU - Bryant, L. K.. AU - Chapman, M. J.. AU - Holloway, R. H.. AU - Mangoni, A. A.. AU - Fraser, R. J.. PY - 2011/4. Y1 - 2011/4. N2 - Objective: Motilin receptors are rapidly down-regulated by exposure to erythromycin, and its progressive loss of clinical prokinetic effect may relate to higher plasma drug concentrations. This study aimed to evaluate the relationship between plasma erythromycin concentrations and feeding outcomes in critically ill patients.Design: Observational comparative study.Setting: Tertiary critical care unit.Patients: Twenty-nine feed-intolerant (gastric residual volume ,250 mL) mechanically ventilated, medical critically ill patients.Interventions: Patients received intravenous erythromycin 200 mg twice daily for feed intolerance.Measurements: Plasma erythromycin concentrations were measured 1 and 7 ...
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The genes for ribosomal proteins L4 and L22 from two erythromycin-resistant mutants of Escherichia coli have been isolated and sequenced. In the L4 mutant, an A-to-G transition in codon 63 predicted a Lys-to-Glu change in the protein. In the L22 strain, a 9-bp deletion removed codons 82 to 84, eliminating the sequence Met-Lys-Arg from the protein. Consistent with these DNA changes, in comparison with wild-type proteins, both mutant proteins had reduced first-dimension mobilities in two-dimensional polyacrylamide gels. Complementation of each mutation by a wild-type gene on a plasmid vector resulted in increased erythromycin sensitivity in the partial-diploid strains. The fraction of ribosomes containing the mutant form of the protein was increased by growth in the presence of erythromycin. Erythromycin binding was increased by the fraction of wild-type protein present in the ribosome population. The strain with the L4 mutation was found to be cold sensitive for growth at 20 degrees C, and ...
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Erythromycin combines the antimicrobial effect and prokinetic properties in Hepatic encephalopathy Liver cirrhosis Erythromycin Neomycin. Cholestasis is a condition where bile cannot flow from the liver to the duodenum. The two basic cholangitis, associated with inflammatory bowel disease; some drugs (e.g.. flucloxacillin and erythromycin) cimetidine, erythromycin, estrogen, and statins can cause cholestasis and may result in damage to the liver. Prescribing should be carefully done in all patients with severe liver disease. The main problems They include macrolides like erythromycin, azithro- mycin. The following antibiotics belong to this family: Erythromycin, Some of these antibiiotics can actually put us into liver failure.. i carry a list in my. Of erythromycin that contains a methoxy substitution at. C-6 of the erythronolide ring (6-0-methylerythromycin). Mild reversible liver injury, predominantly. Clinical manifestations of erythromycin estolate- induced liver disease were severe ...
The current study confirms our earlier findings that erythromycin administered intravenously in healthy volunteers has a dose related effect on the interdigestive motility of the stomach and the small intestine.3 A low dose (40 mg) of erythromycin induced a premature activity front at the antral level which migrated through the small intestine. A higher dose of erythromycin (200 mg) induced strong and prolonged antral contractile activity which was not propagated to the small intestine. The effect of a single dose of atropine or sumatriptan was used to investigate the effect of both treatments on the antral motor effects of two different doses of erythromycin. Prior administration of the muscarinic antagonist atropine blocked the generation of a premature activity front in the stomach by a low dose of erythromycin. However, prolonged antral contractile activity induced by a high dose of erythromycin remained unaffected by atropine. Similar findings were observed after administration of the ...
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TY - JOUR. T1 - Determination of erythromycin concentrations in rat plasma and liver by high-performance liquid chromatography with amperometric detection. AU - Hanada, Erika. AU - Ohtani, Hisakazu. AU - Kotaki, Hajime. AU - Sawada, Yasufumi. AU - Iga, Tatsuji. PY - 1997/5/9. Y1 - 1997/5/9. N2 - A simple method for the quantitative determination of erythromycin (EM) concentrations in rat plasma and liver by high-performance liquid chromatography with amperometric detection was developed. EM was extracted from 200 μl of plasma or liver homogenate sample under sodium hydroxide alkaline conditions with tert.-butyl methyl ether. Oleandomycin was used as an internal standard. The recovery rate of EM was up to 100%. The detector cell potential for the oxidation of EM was +1100 mV. The calibration curves were linear over the concentration ranges 0.1-20.0 μg/ml for plasma and 0.5-100.0 μg/g for liver. The method was applied to the determination of the plasma and liver concentrations of EM in rats ...
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OBJECTIVES: To test four 16-membered macrolides (josamycin, spiramycin, midecamycin and rokitamycin) along with other compounds in the same class (erythromycin, clarithromycin, roxithromycin and azithromycin) plus clindamycin and telithromycin, against Streptococcus pyogenes and Streptococcus pneumoniae isolates with well-characterized resistance genotypes. METHODS: Four hundred and eighty-six isolates of S. pyogenes and 375 isolates of S. pneumoniae were assayed for their macrolide susceptibilities and investigated by PCR to detect their different erythromycin resistance genes. All strains had been isolated over the period 2002-2003 from specimens of different human origin obtained in 14 different Italian centres. RESULTS: All 16-membered macrolides showed very low MICs (MIC(50)s and MIC(90)s, , or =0.06-0.5 mg/L) for the erythromycin-susceptible isolates and for those with the M phenotype, but the telithromycin MICs for the M-type isolates were at least four times higher (MIC(90)s, 0.5 mg/L). ...
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In Sweden, mares sometimes develop acute, often fatal, colitis when their foals are treated orally with erythromycin and rifampicin for Rhodococcus (R.) equi infection. Clostridium (C.) difficile, or its cytotoxin, was demonstrated in faecal samples from 5 of 11 (45%) mares with diarrhoea. By contrast C. difficile was not found in the faecal flora of 12 healthy mares with foals treated for R. equi infection or in 56 healthy mares with healthy untreated foals. No other enteric pathogen was isolated from any diarrhoeic mare. Of 7 investigated treated foals, 4 had a high (1651.0, 1468.3, 273.0 and 88.8 μg/g) faecal concentration of erythromycin. The dams of those 4 foals developed acute colitis, whereas the dams of 3 foals with a lower (26.3, 4.6 and 3.7 μg/g) faecal erythromycin concentration remained healthy, indicating that there might have been an accidental intake of erythromycin by mares. The foals treated with antibiotics were regarded as asymptomatic carriers and potential reservoirs, as ...
The erythromycin breath test (ERMBT) is a method used to measure metabolism (oxidation and elimination from the system) by a part of the cytochrome P450 system. Erythromycin is tagged with carbon-14 and given as an intravenous injection; after 20 minutes the subject blows up a balloon and the carbon dioxide exhaled that is tagged with carbon-14 shows the activity of the CYP3A4 isoenzyme on the erythromycin. Therefore, this activity can predict how other drugs that are metabolized by the CYP3A4 part of the cytochrome P450 system will be acted upon by a particular person. The test allows doctors to determine or predict an individuals drug treatment outcome. Will a patient develop serious or fatal side effects from a certain drug? Which foods and drugs should not be taken together? With this and other tests a physician may determine treatment outcomes in advance or study the effects of new drugs. Some patients have a congenital inability to synthesize certain enzymes, so drugs may build up to ...
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The results of this study showed that in Finland themefA gene is predominant among erythromycin-resistant M-phenotype isolates and the ermTR gene is predominant among isolates with MLSB resistance phenotypes. A comparison of susceptibilities between erythromycin-resistant S. pyogenes isolates in this study and isolates collected in 1990 (28) showed no changes in the antibiotic resistance patterns of the M- and CR-phenotype isolates. However, two interesting changes were seen in the antibiotic resistance pattern of the IR-phenotype isolates. In 1990, the proportion of the IR-phenotype isolates that were resistant to tetracycline was 10% (28), which was only a little more than the proportion found among erythromycin-susceptibleS. pyogenes isolates (4%). However, in 1994 and 1995 (this study), 93% of the IR phenotype isolates were resistant to tetracycline, which is comparable to the 82 and 100% rates of resistance found among the CR-phenotype isolates in 1990 (28) and 1994 and 1995, respectively, ...
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