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TY - JOUR. T1 - Plasma erythromycin concentrations predict feeding outcomes in critically ill patients with feed intolerance. AU - Nguyen, N. Q.. AU - Grgurinovich, N. G.. AU - Bryant, L. K.. AU - Chapman, M. J.. AU - Holloway, R. H.. AU - Mangoni, A. A.. AU - Fraser, R. J.. PY - 2011/4. Y1 - 2011/4. N2 - Objective: Motilin receptors are rapidly down-regulated by exposure to erythromycin, and its progressive loss of clinical prokinetic effect may relate to higher plasma drug concentrations. This study aimed to evaluate the relationship between plasma erythromycin concentrations and feeding outcomes in critically ill patients.Design: Observational comparative study.Setting: Tertiary critical care unit.Patients: Twenty-nine feed-intolerant (gastric residual volume ,250 mL) mechanically ventilated, medical critically ill patients.Interventions: Patients received intravenous erythromycin 200 mg twice daily for feed intolerance.Measurements: Plasma erythromycin concentrations were measured 1 and 7 ...
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Our results indicate that VLBW infants with feed intolerance due to non-anatomically obstructive gastrointestinal dysmotility achieved full enteral feeding significantly earlier after treatment with oral erythromycin. The median times taken to establish half, three quarters, and full enteral nutrition were 3.5, 8.5, and 13.5 days after the start of oral erythromycin. The median times taken to achieve half and full oral feeding in the placebo group were almost twice as long (table2). These findings agree with those of previous case series in preterm infants.13-15 Although a recent randomised controlled study did not show any benefit of intravenous erythromycin in improving the tolerance of enteral feeding in preterm infants,25these findings were not entirely unexpected because the study was not primarily designed to investigate the effectiveness of erythromycin on enteral feeding, but rather focused on the relation between chronic lung disease and intravenous erythromycin treatment for ...
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The genes for ribosomal proteins L4 and L22 from two erythromycin-resistant mutants of Escherichia coli have been isolated and sequenced. In the L4 mutant, an A-to-G transition in codon 63 predicted a Lys-to-Glu change in the protein. In the L22 strain, a 9-bp deletion removed codons 82 to 84, eliminating the sequence Met-Lys-Arg from the protein. Consistent with these DNA changes, in comparison with wild-type proteins, both mutant proteins had reduced first-dimension mobilities in two-dimensional polyacrylamide gels. Complementation of each mutation by a wild-type gene on a plasmid vector resulted in increased erythromycin sensitivity in the partial-diploid strains. The fraction of ribosomes containing the mutant form of the protein was increased by growth in the presence of erythromycin. Erythromycin binding was increased by the fraction of wild-type protein present in the ribosome population. The strain with the L4 mutation was found to be cold sensitive for growth at 20 degrees C, and ...
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The current study confirms our earlier findings that erythromycin administered intravenously in healthy volunteers has a dose related effect on the interdigestive motility of the stomach and the small intestine.3 A low dose (40 mg) of erythromycin induced a premature activity front at the antral level which migrated through the small intestine. A higher dose of erythromycin (200 mg) induced strong and prolonged antral contractile activity which was not propagated to the small intestine. The effect of a single dose of atropine or sumatriptan was used to investigate the effect of both treatments on the antral motor effects of two different doses of erythromycin. Prior administration of the muscarinic antagonist atropine blocked the generation of a premature activity front in the stomach by a low dose of erythromycin. However, prolonged antral contractile activity induced by a high dose of erythromycin remained unaffected by atropine. Similar findings were observed after administration of the ...
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TY - JOUR. T1 - Determination of erythromycin concentrations in rat plasma and liver by high-performance liquid chromatography with amperometric detection. AU - Hanada, Erika. AU - Ohtani, Hisakazu. AU - Kotaki, Hajime. AU - Sawada, Yasufumi. AU - Iga, Tatsuji. PY - 1997/5/9. Y1 - 1997/5/9. N2 - A simple method for the quantitative determination of erythromycin (EM) concentrations in rat plasma and liver by high-performance liquid chromatography with amperometric detection was developed. EM was extracted from 200 μl of plasma or liver homogenate sample under sodium hydroxide alkaline conditions with tert.-butyl methyl ether. Oleandomycin was used as an internal standard. The recovery rate of EM was up to 100%. The detector cell potential for the oxidation of EM was +1100 mV. The calibration curves were linear over the concentration ranges 0.1-20.0 μg/ml for plasma and 0.5-100.0 μg/g for liver. The method was applied to the determination of the plasma and liver concentrations of EM in rats ...
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TY - JOUR. T1 - Proton-coupled erythromycin antiport at rat blood-placenta barrier. AU - Sai, Yoshimichi. AU - Nishimura, Tomohiro. AU - Ochi, Kaori. AU - Tanaka, Noriaki. AU - Takagi, Akinori. AU - Tomi, Masatoshi. AU - Kose, Noriko. AU - Kobayashi, Yasuna. AU - Miyakoshi, Naoki. AU - Kitagaki, Shinji. AU - Mukai, Chisato. AU - Nakashima, Emi. PY - 2010/9. Y1 - 2010/9. N2 - The aim of the present study was to characterize the mechanism of erythromycin transport at the blood-placenta barrier, using TR-TBT 18d-1 cells as a model of rat syncytiotrophoblasts. [14C]Erythromycin was taken up by TR-TBT 18d-1 cells with a Michaelis constant of 466 μM. Although the uptake was not dependent on extracellular Na+ or Cl-, it was increased at weakly alkaline pH. Significant overshoot of [ 14C]erythromycin uptake by placental brush-border membrane vesicles was observed in the presence of an outwardly directed proton gradient. These results indicate that erythromycin is transferred by the H+-coupled transport ...
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