Protocol for preparing red blood cell ghosts - posted in Cell Biology: Hi all, I need to make RBC ghosts for running my experiments. I basically need to get the hemoglobin out of the rbcs and reseal them. As have the hemoglobin puts a limitation on the hematocrit I can use for my experiments. I would really appreciate if one of you could give me a detailed protocol. I am a mechanical engineer and that makes these processes a little more complicated than they are. For example, I followed...
Klara Pecankova, Pavel Majek, Jaroslav Cermak, Jan E. Dyr. Posttranslational Modifications of Red Blood Cell Ghost Proteins as Signatures for Distinguishing between Low- and High-Risk Myelodysplastic Syndrome Patients. Turk J Hematol. 2017; 34(1): 111- ...
TY - JOUR. T1 - Large scale isolation of human erythrocyte membranes by high volume molecular filtration. AU - Rosenberry, Terrone L.. AU - Chen, Jeffrey F.. AU - Lee, Mary M.L.. AU - Moulton, Thomas A.. AU - Onigman, Philip. PY - 1981/1. Y1 - 1981/1. N2 - A molecular filtration procedure for preparing large quantities of human erythrocyte ghost membranes is presented. Hemolysate ghost membranes are rapidly cycled in the retantate channel of the filtration apparatus, while hemoglobin is removed s it pass through Pellicon filters into the filtrate. Several-liter quantities of washed packed erythrocytes can be processed in a few hours with this system and the filtration procedure does not appear to alter erythrocyte or ghost membranes. Intact erythrocytes in isotonic solution can be circulated through the retentate channel for 16 h with only 3% hemolysis and with preservation of their orginal morphology in scanning electron microscopy. Ghost membranes isolated by the procedure are virtually ...
TY - JOUR. T1 - Scanning tunneling microscopy of human erythrocyte membranes. AU - Gaczynska, M.. AU - Chwialkowski, M.. AU - Olejniczak, W.. AU - Wojczuk, S.. AU - Bartosz, G.. PY - 1991/12/16. Y1 - 1991/12/16. N2 - Images of surfaces of human erythrocyte ghosts, lecithin liposomes, spectrin, erythrocyte membrane skeleton, concanavalin A and concanavalin A - decorated erythrocyte ghosts were obtained by scanning tunneling microscopy. The dimensions and surface topography of some membrane structures are described and discussed.. AB - Images of surfaces of human erythrocyte ghosts, lecithin liposomes, spectrin, erythrocyte membrane skeleton, concanavalin A and concanavalin A - decorated erythrocyte ghosts were obtained by scanning tunneling microscopy. The dimensions and surface topography of some membrane structures are described and discussed.. UR - http://www.scopus.com/inward/record.url?scp=0026344388&partnerID=8YFLogxK. UR - ...
Red blood cell (RBC) physiology is directly linked to many human disorders associated with low tissue oxygen levels or anemia including chronic obstructive pulmonary disease, congenital heart disease, sleep apnea and sickle cell anemia. Parasites such as Plasmodium spp. and phylum Apicomplexa directly target RBCs, and surface molecules within the RBC membrane are critical for pathogen interactions. Proteomics of RBC membrane ghost fractions has therefore been of considerable interest, but protocols described to date are either suboptimal or too extensive to be applicable to a larger set of clinical cohorts. Here, we describe an optimised erythrocyte isolation protocol from blood, tested for various storage conditions and explored using different fractionation conditions for isolating ghost RBC membranes. Liquid chromatography mass spectrometry (LC-MS) analysis on a Q-Exactive Orbitrap instrument was used to profile proteins isolated from the comparative conditions. Data analysis was run on the MASCOT
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A commonly used measure to reflect the intake of the long-chain omega-3 fatty acids EPA and DHA is the omega-3 index, defined as the sum of EPA + DHA as % of total fatty acids in erythrocyte membrane. When the omega-3 index changes it follows that the relative fractions of other fatty acids in the membrane are also changed. In the present study, increasing doses of a preparation of omega-3 rich phospholipids extracted from krill oil were administered orally to non-human primates for 12 weeks and the time course of EPA, DHA and 22 other fatty acids in erythrocytes was determined bi-weekly during treatment and for 8 weeks after cessation of treatment. Plasma concentrations of six endocannabinoid-type mediators being downstream metabolites of some fatty acids analyzed in erythrocytes were also determined. Six diabetic, dyslipidemic non-human primates were included, three in a vehicle control group and three being treated with the omega-3 rich phospholipid preparation. The vehicle control and test items
A commonly used measure to reflect the intake of the long-chain omega-3 fatty acids EPA and DHA is the omega-3 index, defined as the sum of EPA + DHA as % of total fatty acids in erythrocyte membrane. When the omega-3 index changes it follows that the relative fractions of other fatty acids in the membrane are also changed. In the present study, increasing doses of a preparation of omega-3 rich phospholipids extracted from krill oil were administered orally to non-human primates for 12 weeks and the time course of EPA, DHA and 22 other fatty acids in erythrocytes was determined bi-weekly during treatment and for 8 weeks after cessation of treatment. Plasma concentrations of six endocannabinoid-type mediators being downstream metabolites of some fatty acids analyzed in erythrocytes were also determined. Six diabetic, dyslipidemic non-human primates were included, three in a vehicle control group and three being treated with the omega-3 rich phospholipid preparation. The vehicle control and test items
The action of purified phospholipases on monomolecular films of various interfacial pressures is compared with the action on erythrocyte membranes. The phospholipases which cannot hydrolyse phospholipids of the intact erythrocyte membrane, phospholipase C from Bacillus cereus, phospholipase A2 from pig pancreas and Crotalus adamanteus and phospholipase D from cabbage, can ... read more hydrolyse phospholipid monolayers at pressure below 31 dynes/cm only. The phospholipases which can hydrolyse phospholipids of the intact erythrocyte membrane, phospholipase C from Clostridium welchii phospholipase A2 from Naja naja and bee venom and sphingomyelinase from Staphylococcus aureus, can hydrolyse phospholipid monolayers at pressure above 31 dynes/cm. It is concluded that the lipid packing in the outer monolayer of the erythrocyte membrane is comparable with a lateral surface pressure between 31 and 34.8 dynes/cm. show less ...
TY - JOUR. T1 - Biochemical and morphological properties of bovine erythrocyte membrane glycoproteins. AU - Fletcher, M. A.. AU - Brunschwig, J. P.. AU - Lo, H.. AU - Caldwell, K. E.. AU - Lo, T. M.. PY - 1982. Y1 - 1982. N2 - The major and minor sialoglycoproteins of the bovine erythrocyte have been solubilized and extensively purified. A comparison of composition revealed that the major glycoprotein had 77% carbohydrate and 23% peptide, and the minor one had 27% carbohydrate and 73% peptide. Molar ratios of sugars were related, however, the major glycoprotein had twice as much galactose and sialic acid as did the minor glycoprotein. Molecular weights, estimated from retardation coefficients of mobility in sodium dodecyl sulfate gel electrophoresis, were 55,000 for the major glycoprotein and 34,000 for the minor glycoprotein. The glycoproteins were studied by electron microscopy before and after delipidation and after ultracentrifugation. The major glycoprotein, prior to delipidation, formed ...
Saleemuddin, M.; Zimmermann, U.; Schneeweiss, F., 1977: Preparation of human erythrocyte ghosts in isotonic solution hemo globin content and poly peptide composition
Alnakshbandi, Abdulkadir A. (2015) Aminoglycosides induce fragility of human red cell membrane: An in vitro study. [Publication] Full text not available from this repository ...
We have measured Ca binding to fragmented human red cell membranes under equilibrium conditions in the presence of low concentrations of EGTA-buffered, ionized Ca. The ionic strength of the assay...
Lipoprotein metabolism influenced by training-induced changes in human skeletal muscle. Differing erythrocyte membrane skeletal protein defects in alpha and beta thalassemia
The article summarizes new insights into the molecular mechanisms for the maintenance and regulation of the asymmetric distribution of phospholipids in human erythrocyte membranes. We focus on phosphatidylserine, which is primarily found in the inner leaflet of the membrane lipid bilayer under low C...
Read "The role of multidrug resistance protein 1 (MRP1) in transport of fluorescent anions across the human erythrocyte membrane, The Journal of Membrane Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Abstract: Amount of cholesterol was distinctly decreased but content of scvalene, lanosterol, 7-dehydrocholesterol and 7-beta-hydroxycholesterol--increased in erythrocyte membranes of rats with Shvetz experimental leukosis. At the same time, osmotic stability of erythrocytes and a pattern of acidic erythrogramms were altered. After UV irradiation of rats amount of cholesterol was increased as well as other unidentified substances appeared in erythrocyte membranes. These alterations appear to be one of factors responsible for development of body resistance to leukosis ...
Resealed Erythrocytes as Drug Carriers and Its Therapeutic Applications: 10.4018/978-1-5225-0754-3.ch012: In this pharma innovative world, there are more than 30 drug delivery systems. Todays due to lacking the target specificity, the present scenario about drug
The flow method of reaction rate measurement has been adapted to the determination of the rate of diffusion of water into the human red cell. In seven experiments the half-time for diffusion exchange has been found to be 4.2 ± 1.1 msec., which is equivalent to a diffusion flow of 8.6 x 10-9 ml. H2O/(sec., red cell). This figure has been compared with the rate of water entrance under an osmotic pressure gradient, and has been found to be smaller by a factor of 2.5. The difference between these two rates of water entrance has been interpreted as indicating the presence of water-filled channels in the membrane. An estimate of the equivalent radius of these channels (on the assumption of uniform right cylindrical pores) leads to a value of 3.5 Å, which is viewed as an operational description of the resistance offered by the membrane to the passage of water.. ...
TY - JOUR. T1 - Control of the erythrocyte membrane shape. T2 - Recovery from the effect of crenating agents. AU - Alhanaty, E.. AU - Sheetz, Michael. PY - 1981/1/1. Y1 - 1981/1/1. N2 - Intact erythrocytes become immediately crenated upon addition of 2,4-dinitrophenol (DNP) or pyrenebutyric acid (PBA). However, when cells are incubated at 37° C in the presence of the crenating agents with glucose, they gradually (4-8 h) recover the normal biconcave disc form. The recovery process does not reflect a gradual inactivation of DNP or PBA since fresh cells are equally crenated by the supernatant from the recovered cells. Further, after recovery and removal of the crenating agents, cells are found to be desensitized to the readdition of DNP as well as to the addition of PBA, but they are more sensitive to cupping by chlorpromazine. This alteration in the cell membrane responsiveness was reversible upon further incubation in the absence of DNP. Recovery is dependent upon cellular metabolic state since ...
Clone REA368 recognizes the human CD233 antigen, a multi-pass membrane protein also known as anion exchange protein 1 (AE1) or solute carrier family 4 member 1 (SLC4A1). CD233 is a phylogenetically preserved transport protein responsible for mediating the electroneutral anion exchange of chloride for bicarbonate across a plasma membrane. It is the major integral membrane glycoprotein of the erythrocyte membrane and is required for the normal flexibility and stability of the erythrocyte membrane as well as for the normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. CD233 mediates the chloride-bicarbonate exchange in the kidney, and is required for the normal acidification of the urine. Additional information: Clone REA368 displays negligible binding to Fc receptors. - Belgique
Flotillins are prominent, oligomeric protein components of erythrocyte (RBC) membrane raft domains and are considered to play an important structural role in lateral organization of the plasma membrane. In our previous work on erythroid membranes and giant plasma membrane vesicles (GPMVs) derived from them we have shown that formation of functional domains (resting state rafts) depends on the presence of membrane palmitoylated protein 1 (MPP1/p55), pointing to its new physiological role. Exploration of the molecular mechanism of MPP1 function in organizing membrane domains described here, through searching for its molecular partners in RBC membrane by using different methods, led to the identification of the raft-marker proteins, flotillin 1 and flotillin 2, as hitherto unreported direct MPP1 binding-partners in the RBC membrane ...
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Complete information for EPB41L3 gene (Protein Coding), Erythrocyte Membrane Protein Band 4.1 Like 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for EPB41L3 gene (Protein Coding), Erythrocyte Membrane Protein Band 4.1 Like 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Homo sapiens erythrocyte membrane protein band 4.1-like 1 (EPB41L1), transcript variant 2, mRNA. (H00002036-R02) - Products - Abnova
The structure and function of the red cell membrane and associated ion transporters play an important role in the pathology of red cell genetic defects
This bald eagle spotted a dead fish floating on a lake and tried several times to swoop in and fly away with it. But the fish seems to be quite heavy, so t...
Looking for online definition of 40-kDa erythrocyte membrane protein in the Medical Dictionary? 40-kDa erythrocyte membrane protein explanation free. What is 40-kDa erythrocyte membrane protein? Meaning of 40-kDa erythrocyte membrane protein medical term. What does 40-kDa erythrocyte membrane protein mean?
Soejima A, Matsuzawa N, Miyake N, Karube M, Fukuoka K, Nakabayashi K, Kitamoto K, Nagasawa T.. Clin Nephrol 1999 Feb;51(2):92-7. BACKGROUND: Persistent hypoalbuminemia is a long-term poor prognostic factor in chronic hemodialysis patients. PATIENTS AND METHODS: We investigated the correlation between the degree of peroxidation of erythrocyte membrane lipids, erythrocyte alpha tocopherol content, erythrocyte glutathione peroxidase activity and serum albumin concentration in twelve patients with uremia not undergoing hemodialysis and fifteen patients on maintenance hemodialysis. RESULTS: The glutathione peroxidase activity in erythrocytes was higher in patients of uremia not undergoing hemodialysis than in chronic hemodialysis patients. A significant negative correlation was observed between the erythrocyte alpha tocopherol content and the degree of erythrocyte membrane lipid peroxidation in chronic hemodialysis patients. There was a statistically significant difference in the degree of ...
TY - JOUR. T1 - Model of red blood cell membrane skeleton. T2 - Electrical and mechanical properties. AU - Kozlov, M. M.. AU - Markin, V. S.. PY - 1987/12/21. Y1 - 1987/12/21. N2 - A theoretical membrane skeleton model of erythrocyte has been developed and successfully applied to interpret electrical and mechanical properties of the red blood cell spectrin-actin network. The model is based on the structure of the membrane skeleton that is comprised of unit cells each containing an actin protofilament and shooting forth a few spectrin heterodimers. The loose ends of the heterodimers of adjacent cells can form bonds with each other giving rise to an integrated network. The number of bonds depends on the temperature. The bond length being excessive (2·6 times the distance between the centers of adjacent cells), the bonds are flexible, and can thus be regarded as entropy springs. The advanced model has been employed to calculate the shear modulus of the membrane skeleton as well as to establish its ...
Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic microscopy (DHM). This interferometric and dye-free technique, possessing nanometric axial and microsecond temporal sensitivities enables to measure cell membrane fluctuations (CMF) on the whole cell surface. DHM acquisition is combined with a model which allows extracting the membrane fluctuation amplitude, while taking into account cell membrane topology. Uneven distribution of CMF amplitudes over the RBC surface is observed, showing maximal values in a ring corresponding to the highest points on the RBC torus as well as in some scattered areas in the inner region of the RBC. CMF amplitudes of 35.9+/-8.9 nm and 4.7+/-0.5 nm (averaged over the cell surface) were determined for normal and ethanol-fixed RBCs, respectively.
1) We have prepared murine monoclonal antibodies to the membrane domain of the human erythrocyte anion transport protein (band 3). (2) All of these antibodies react with regions of the protein located at the cytoplasmic surface of the red cell. (3) One of the antibodies reacts with an epitope present on a cytoplasmic loop of the protein located between the C-terminus and a point 168 amino acids from the C-terminus. The other antibodies recognize different epitopes on the C-terminal tail of the protein and the sequences likely to be involved in these epitopes are defined. (4) Our results show that the C-terminus of the red-cell anion transport protein is located on the cytoplasmic side of the red-cell membrane. (5) None of the antibodies inhibited sulphate exchange transport when introduced into resealed red-cell membranes; however, the bivalent form of one of the antibodies reduced the inhibitory potency of 4-acetamido-4-isothiocyanatostilbene disulphonate on sulphate exchange transport in ...
The enzyme lactoperoxidase was used to catalyse the radioiodination of membrane proteins in intact human erythrocytes and in erythrocyte ghosts. Two major proteins of the erythrocyte membrane were isolated after iodination of these two preparations, and the peptide maps of each protein so labelled were compared. Peptides from both proteins are labelled in the intact cell. In addition, further mobile peptides derived from one of the proteins are labelled only in the ghost preparation. Various sealed ghost preparations were also iodinated, lactoperoxidase being present only at either the cytoplasmic or extra-cellular surface of the membrane. The peptide maps of protein E (the major membrane protein) labelled in each case were compared. Two discrete sets of labelled peptides were consistently found. One group is obtained when lactoperoxidase is present at the extra-cellular surface and the other group is found when the enzyme is accessible only to the cytoplasmic surface of the membrane. ...
The distribution of specific glycoprotein receptors on the external surfaces of red cells was mapped, by the freeze-etching technique, to determine if the receptors coincided with the underlying 75-A intramembranous particles. Phytohemagglutinin, ferritin-conjugated phytohemagglutinin, and influenza virus were used as labeling agents since they can be seen by freeze-etching techniques and each reacts with a different site on the same glycoprotein molecule. The distribution of these labels was studied on intact human red cells, isolated ghost membranes, and trypsin-treated ghost membranes.. The results show that the receptors for these labels are distributed uniformly over the surfaces of normal red cell membranes in the same apparent distribution as that of the 75-A particles within the membrane. The association between the external receptors and the underlying particles is especially evident when trypsin-treated ghost membranes are labeled: the labeled receptor sites and the intramembranous ...
The authors evaluated the role of a hyperproteic, hypocaloric, polyunsaturated fatty acid (PUFA) supplemented diet on anthropometric parameters, erythrocyte membrane fatty acid composition and plasma antioxidant defences of non professional volleyball athletes. The athletes were divided in two groups: One (n=5) followed the Mediterranean diet, and the other (n=6) followed a high protein, low calorie diet with a 3g/day fish oil supplementation. All the athletes had anthropometric measurements taken, both at the beginning and at the end of the study, which lasted for 2 months. Body mass index and total body fat were significantly diminished in the second group, while they remained unchanged in the first. Plasma total antioxidant activity (TAA) was significantly increased in the plasma of both groups, with no differences between the groups, suggesting that physical activity, not the different diets, is the main contributor to the increase of plasma TAA. The second group showed a significant ...
Zhang, J, Tu, K, Xu, Y, Pan, L, Wu, C, Chen, X, Wu, M, Cheng, Z and Chen, B (2013) Sphingomyelin in erythrocyte membranes increases the total cholesterol content of erythrocyte membranes in patients with acute coronary syndrome. ...
Top performende anti-Maus erythrocyte Membrane Protein Band 4.9 (Dematin) Antikörper für Immunochromatography (IC) vergleichen & kaufen.
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Native spectrin molecule is a tetramer composed of two antiparallel heterodimers joined head to head so that each end of the native molecule includes the C-terminus of the alpha subunit and the N-terminus of the beta subunit ...
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A plasma membrane fraction of HeLa S3 cells, consisting of ghosts, is characterized more fully. A simple procedure is described which permits light and electron microscope study of the plasma membrane fraction through the entire depth of the final product pellet and through large areas parallel to the surface. Contamination by nuclei is 0.14%, too little for DNA detection by the diphenylamine reaction. Contamination by rough endoplasmic reticulum and ribosomes is small, a single ghost containing about 3% of the RNA in a single cell. Mitochondria were not encountered. Electron microscopy also shows (a) small vesicles associated with the outer surface of the ghosts, and (b) a filamentous web at the inner face of the ghost membrane. Sodium dodecyl sulfate (SDS)-polyacrylamide gel analysis shows that of the many Coomassie Blue-stained bands two were prominent. One, 43,000 daltons, co-migrated with purified rabbit muscle actin and constituted about 7.5% of the plasma membrane protein. The other major ...
Main parameters of lipid complex were studied in erythrocytes of whole blood and of the blood containing anticoagulant. Initial steps of blood coagulation involved activation of erythrocyte endogenous phospholipase A, which led to destabilization of erythrocyte lipid structures as a result of an increase in concentration of free fatty acids, accumulation of lysophospholipids as well as of alterations in microviscosity of erythrocyte membranes ...
The nanosponges look like red blood cells, and therefore serve as red blood cell decoys that collect the toxins. The nanosponges absorb damaging toxins and divert them away from their cellular targets. The nanosponges had a half-life of 40 hours in the researchers experiments in mice. Eventually the liver safely metabolized both the nanosponges and the sequestered toxins, with the liver incurring no discernible damage.. Each nanosponge has a diameter of approximately 85 nanometers and is made of a biocompatible polymer core wrapped in segments of red blood cells membranes.. Zhangs team separates the red blood cells from a small sample of blood using a centrifuge and then puts the cells into a solution that causes them to swell and burst, releasing hemoglobin and leaving RBC skins behind. The skins are then mixed with the ball-shaped nanoparticles until they are coated with a red blood cell membrane.. Just one red blood cell membrane can make thousands of nanosponges, which are 3,000 times ...
I am pleased to have had the opportunity to present an overview of red cell membranes in normal and disease states with my background of nearly 30 years in this area of research. I believe that this kind of publication on red cell membrane is a very timely summary of all the results obtained by the tremendous efforts worldwide by all of the scientists in this field during the past few decades. As reviewed in Chapter 1, the general concepts of red cell membrane abnormalities and the categories of each red cell membrane disorder are now well established. ...
Human protein 4.2 (P4.2) is a major membrane skeletal protein in erythrocytes. Individuals with P4.2 deficiency exhibit spherocytosis and experience various degrees of hemolytic anemia, suggesting a role for this protein in maintaining stability and integrity of the membrane. Molecular cloning of P4.2 cDNAs showed that P4.2 is a transglutaminaselike molecule in erythrocytes but lacks the essential cysteine for cross-linking activity. Two cDNA isoforms have been identified from a human reticulocyte cDNA library, with the long isoform containing a 90-base pair (bp) in-frame insertion encoding an extra 30 amino acids near the N-terminus. Characterization of the P4.2 gene suggests differential splicing as the mechanism for generating these two cDNA isoforms. The donor site for the short isoform (P4.2S) agrees better with the consensus than the donor site for the long isoform (P4.2L) does. Expression of P4.2L was detected by a long- isoform-specific antibody raised against a peptide within the ...
There are conflicting results regarding the erythrocyte membrane cholesterol and phospholipid content in patients with primary hypercholesterolemia (PHC), due to methodological problems in obtaining haemoglobin-free ghosts. At the same time, the diff
The effectiveness of penetration of erythrocyte membrane by sodium salt of 2,4-dichlorophenoxyacetic acid was analyzed. The experiment was executed in a dependence on different doses of the herbicide and at different times of incubation of red blood cells with 2,4-D-Na. It is known that...
The biomembrane is postulated as the initial target when Platinum(II) complexes attack cells. In this work, a spin-labeling ESR technique has been used to study the effects of cis-DCDP, cis-DBDP, cis-DIDP, trans-DCDP, and cis-DADP on the permeability of human erythrocyte membrane. We monitored the reduction processes of the ESR signal of a nitroxide spin label, (TEMPO), which leaks out through the membrane and is reduced by the external ascorbate. Our results indicate that cisplatin and its analogues can enhance the permeability of membranes to small moieties such as TEMPO and ascorbate, and the differences between these compounds are related to features of the leaving group. In addition, changes in the order parameter of 5DS spin label in membrane indicate that hydrolysis of these Pt(II) complexes result in membrane damage.. ...
Anemia is a condition that has multiple origins. One such origin is the destruction of red blood cells (RBCs) membrane induced by free radicals. Treatment of anemia could therefore be enhanced by the use of free radicals scavengers potentially found in some medicinal plants. In this study, the protective effect of Harungana madagascariensis on the RBCs membrane physiology was investigated in vitro and in vivo. In vitro hemolytic anemia was induced by incubation of fresh human RBCs with carbontetrachloride (CCl4) in Olive oil (Oo). Relaxation times of protons excited at 20 MHz (Carr-Purcell-Meiboom-Gill pulse sequence) in the absence or presence of paramagnetic Mn2+ ions (T 2i for
... are a suspension of stabilized erythrocytes of humans and mammals, obtained from erythrocyte mass of humans or whole blood of mammals.. The volume of erythrocytes and their number are preserved throughout the product validity period with the minimal deviations from the passport values, due to the method of effective stabilization developed by our company.. It is possible to obtain stabilized erythrocytes with different antigenic status (ABO system, Rh-factor, other anti-gene systems of human and mammal blood).. Stabilized erythrocytes can be used in the production of control materials for in vitro diagnostics, hematological research, for example, during the production of hematological controls for automatic hematological analyzers, including the possibility of determining normoblasts and reticulocytes.. Storage temperature: +2 +8°С.. Validity period: not less than 180 days.. Each series of stabilized erythrocytes is accompanied by a passport, with ...