The vast majority of therapeutic antibodies have a conformational or discontinuous epitope. Pepscan developed its proprietary CLIPSTM technology to construct constrained peptides which enable the 3D spatial conformation of these epitopes to be addressed. Our epitope mapping platform allows you to identify any type of epitope in a cost-effective and timely manner.. Pepscan developed this epitope mapping platform using solid support with a proprietary hydrogel matrix into which peptide sequences are directly synthesized using robust Fmoc peptide synthesis in a high density. This results in highly sensitive arrays and enables reliable detection of even the weakest binding signals. These immobilized peptide arrays are reusable and can be tested multiple times. This therefore permits screening of a series of antibodies or sera on a single array, making linear epitope mapping a fast and cost-effective option for best candidate selection and further development.. ...
Pleiner T, Bates M, Trakhanov S, Lee CT, Schliep JE, Chug H, Böhning M, Stark H, Urlaub H, Görlich D. 2015. Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation. eLife 4:e11349. doi: 10.7554/eLife.11349.. Published December 3, 2015. An error was identified in Figure 6d (bottom right panel). The image of XL177 cells stained with the Alexa Fluor 647 maleimide-labeled anti-Nup93 nanobody mistakenly duplicated the neighbouring Nup98 image. The error occurred during figure assembly in Adobe Illustrator.. The corrected Figure 6 is shown here:. ...
Epitope Mapping covers all the major methods for the identification and definition of epitopes. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells. The identification of combined B and T cell epitopes can also be achieved using synthetic peptides.
Virus-like particles (VLPs) formed by the human papillomavirus (HPV) L1 capsid protein are currently being tested in clinical trials as prophylactic vaccines against genital warts and cervical cancer. The efficacy of these vaccines is critically dependent upon L1 type-specific conformational epitopes. To investigate the molecular determinants of the HPV16 L1 conformational epitope recognized by monoclonal antibody 16A, we utilized a domain-swapping approach to generate a series of L1 proteins composed of a canine oral papillomavirus (COPV) L1 backbone containing different regions of HPV16 L1. Gross domain swaps, which did not alter the ability of L1 to assemble into VLPs, demonstrated that the L1 N-terminus encodes at least a component of the 16A antigenic determinant. Finer epitope mapping, using GST-L1 fusion proteins, mapped the 16A epitope to the L1 variable regions I and possibly II within the N-terminus. These results suggest that non-contiguous loop regions of L1 display critical components of a
P.C. ,PC at no.email.sorry, wrote in message news:,c0ar25$n7$1 at cruncher.dfci.harvard.edu,... , you mentioned that your deletions are tagged - the best control here is , to blot with anti-tag Ab the same set of deletions. No one can ask for , more. ...already done , , It is likely not the case, but theoretically your epitope still can be , conformational - protein can refold on the membrane. In fact, change , from SDS (or more commonly sarcosyl) into Triton is one of the , renaturation methods. ...this is very good!:) the feeling becomes stronger:) ...of course it could be renaturated on my PVDF:)).. it makes sense and very logic, and this means i have more work to do.... but if ill get a native antibody with that sort of affinity that will be awesome:)) , , Does your antibody IP native protein? IP with your Ab, detect on the , western with anti-tag and you shall be free of your intrinsic feeling! ... to be done next, after linear epitope mapping is completed ... then i wont have any feeling ...
A panel of anti-Fas mAbs bind FasthrRγ1 and FasthrRγ1 mutants. Fusion proteins were immobilized, and binding was detected by anti-Fas mAbs at various concen
EZ-Tn5™ , EZ-Tn5™ , and EZ-Tn5™ Insertion Kits: Insert selectable marker for many applications, including DNA sequencing and functional epitope mapping.
This tool enables users to analyze the T-cell or B-cell epitopes domains, epitope frequency, and red-colored epitope domains on protein 3D structure image. ...
Major criticism: Argument is that by targeting a highly conserved epitope, you can eliminate all quasispecies at once. The idea is, of course, that a highly conserved epitope is so critical that the virus cannot survive without it, so it will be present across all strains. You then go on to do some modeling/combinatorial work to predict common escape mutants. These escape mutants should also require this highly conserved epitope in order to be viable (otherwise the epitope isnt really that conserved). So either the planning for escape mutants should be unnecessary or if there are escape mutants, that means the domain youve targeted isnt so highly conserved (and suggests that the quasispecies problem may not be solved by that epitope). Either way, I think that planning for the possibility of escape mutants undermines the "broadly neutralizing antibody" thrust. Even so, if you were to plan for these mutants, then the antibodies you develop to attack the mutants could be similarly ineffective. ...
உணவு மற்றும் சூழலில் காணப்படும் பாக்டீரியா, வைரசு, மற்றும் சில தாவர பிறபொருளெதிரியாக்கிகள், A மற்றும் B கிளைக்கோ புரத பிறபொருளெதிரியாக்கிகளைப் போன்ற, எபிடோப்களைக் (Epitope) கொண்டுள்ளன. இந்த epitope களே பிறபொருளெதிரியாக்கிகளில் காணப்படும், பிறபொருளெதிரிகளால் அடையாளப்படுத்தக் கூடிய பகுதியாகும். பிறந்து முதல் வருட காலத்தில், இந்த சூழல் பிறபொருளெதிரியாக்கிகளுக்கு எதிராக உடலில் ...
Thirty percent of patients with severe hemophilia treated with factor fVIII (fVIII) develop anti-fVIII antibodies (inhibitors). This is the most significant com...
Looking for online definition of conformational epitope in the Medical Dictionary? conformational epitope explanation free. What is conformational epitope? Meaning of conformational epitope medical term. What does conformational epitope mean?
The use of antigen fragments generated by specific proteolytic cleavage is a relatively simple library approach for epitope mapping in which possible overlapping fragments are screened with the antibody on Western blots. Proteolytic fragmentation with numerous proteases having different cleavage specificites can be carried out on native and denaturated proteins, generating a small and large number of fragments, respectively. To determine the antigenic site of a monoclonal antibody, we have examined the limited proteolytic digestion of the transducin alpha-subunit with four different proteases and detected antibody binding to fragments by Western blot. Using this approach, the epitope for this antibody was localized within the amino-terminal 17 residues of transducin alpha-subunit.. ...
Monoclonal antibodies for dystrophin analysis: epitope mapping and improved binding to SDS treated muscle sections is an eagle-i resource of type Journal article at eagle-i Network Shared Resource Repository.
Here, the authors present a simple and efficient protocol to define a linear antigenic epitope using a purified monoclonal antibody and ...
TY - JOUR. T1 - Peptide epitope identification for tumor-reactive CD4 T cells. AU - Kobayashi, Hiroya. AU - Celis, Esteban. PY - 2008/4/1. Y1 - 2008/4/1. N2 - Because T lymphocytes have the capacity to recognize tumor cells, significant efforts are being devoted towards the development of T cell-based immunotherapy for cancer. Most of this work has centered in the induction of anti-tumor CD8 T cells, which exhibit cytolytic activity towards tumor cells expressing tumor-specific or tumor associated antigens. Unfortunately to this day, T cell-based immunotherapy for cancer remains suboptimal. One of the possible explanations is that these immunotherapies have ignored the role that CD4 T helper lymphocytes play in the generation and persistence of CD8 T cell responses. Thus, we believe that in order to obtain clinical benefits T cell-based immunotherapy must stimulate both CD8 and CD4 tumor-reactive T cell responses. During the past seven years our group has focused on the identification of CD4 T ...
The unique property of specific high-affinity binding to more or less any target of interest has made antibodies tremendously useful in numerous applications. Hence knowledge of the precise binding site (epitope) of antibodies on the target protein is one of the most important features for understanding its performance and determining its reliability in immunoassays. Here, we describe a high-resolution method for mapping epitopes of antibodies based on bacterial surface expression of antigen fragments followed by antibody-based flow cytometric sorting. Epitopes are determined by DNA sequencing of the sorted antibody-binding cells followed by sequence alignment back to the antigen sequence. The method described here has been useful for the mapping of both monoclonal and polyclonal antibodies with varying sizes of epitopes.. ...
Anti-HTT antibody epitopes and analysis by immunoblot.(A) Diagram representing antibody epitopes on human HTT protein (relative to GenBank accession CAD38447.1)
Greenbaum JA, Andersen PH, Blythe M, Bui HH, Cachau RE, Crowe J, Davies M, Kolaskar AS, Lund O, Morrison S, Mumey B, Ofran Y, Pellequer...
Hiv, Infection, Antibodies, Hiv Infection, Epitopes, Igg, Hiv-1, Homeostasis, Neutralizing Antibodies, Iga, Play, Autoimmune Responses, Cd4 Molecule, Epitope Mapping, Genetic Drift, Immune System, Immunity, Immunization, Lymphocyte, Lymphocyte Subset
A Proliferation Inducing Ligand (APRIL) is a TNF ligand that, via its receptors TACI and BCMA, is involved in both B cell physiology as well as in proliferation and survival of malignant B cells. To target APRIL-dependent stimulation of B cell cancers, we recently produced and characterized two monoclonal antagonistic anti-human APRIL antibodies called humanAPRIL.01A (hA.01A) and humanAPRIL.03A (hA.03A). In a first biochemical assay to validate their blocking activity, hA.01A was shown to fully prevent APRIL from binding to its receptors, whereas a substantial difference was detected for hA.03A, which inhibited APRIL binding to BCMA less efficiently than hA.01A. Epitope mapping subsequently revealed that hA.01A and hA.03A bind distinct sites on APRIL, which provided a structural rationale of their different blocking activities. Importantly, this differential inhibition profile can be used to functionally dissect BCMA and TACI-dependent signals and indicated that B cell survival and IgA ...
However, the ability to multiplex these various isotypes and in following recent FDA recommendations, performing epitope mapping of the protein of interest, yields the highest quality results from a single sample in one or a few wells provides many advantages. The advantages include reduction in human error, variability from one assay to the next, significant reduction in labor and method development cost, as well as getting the most detailed information in a single reaction. Choosing a technology that has also shown to have superior drug tolerance with minimal matrix interference also deserves attention ...
This epitope map visualizes all epitope residues associated with the mAb on to the structure of the DENV E protein. Epitope residues are depicted as spheres, colored according to domain, and with a size corresponding to the epitope resolution, for domain, peptide, and residue-level epitopes ...
Pk (V5) Epitope Tag (GKPIPNPLLGLDST), 1 mg. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
Pk (V5) Epitope Tag (GKPIPNPLLGLDST), 0.1 mg. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
|p|The Epitope Tag (Small Motif) Antibody Sampler Kit provides a convenient resource to detect small Epitope Tag fused protein by Western Blot. The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of pro
TY - JOUR. T1 - Epitope Mapping of SERCA2a Identifies an Antigenic Determinant That Induces Mainly Atrial Myocarditis in A/J Mice. AU - Krishnan, Bharathi. AU - Massilamany, Chandirasegaran. AU - Basavalingappa, Rakesh H.. AU - Gangaplara, Arunakumar. AU - Rajasekaran, Rajkumar A.. AU - Afzal, Muhammad Z.. AU - Khalilzad-Sharghi, Vahid. AU - Zhou, You. AU - Riethoven, Jean-Jack M. AU - Nandi, Shyam S.. AU - Mishra, Paras Kumar. AU - Sobel, Raymond A.. AU - Strande, Jennifer L.. AU - Steffen, David J. AU - Reddy, N R Jayagopala. PY - 2018/1/15. Y1 - 2018/1/15. N2 - Sarcoplasmic/endoplasmic reticulum Ca2+ adenosine triphosphatase (SERCA)2a, a critical regulator of calcium homeostasis, is known to be decreased in heart failure. Patients with myocarditis or dilated cardiomyopathy develop autoantibodies to SERCA2a suggesting that they may have pathogenetic significance. In this report, we describe epitope mapping analysis of SERCA2a in A/J mice that leads us to make five observations: 1) SERCA2a ...
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important pathogens, that hinder the development of global pork industry. Its nonstructural protein 11 (nsp11), with the nidoviral uridylate-specific endoribonuclease (NendoU) domain, is essential for PRRSV genome replication and it also contributes to host innate immunity suppression. However, the immunogenicity and immune structure of PRRSV nsp11 have not been well investigated yet. In this study, a monoclonal antibody (mAb), designated 3F9, that against nsp11 was generated. Subsequently, a series of partially overlapped fragments, covered the nsp1140-223aa, were expressed to test the reactivity with mAb 3F9, and the 111DCREY115 was found to be the core unit of the B-cell epitope recognized by mAb 3F9. Further investigation indicated that both genotype 1 and genotype 2 PRRSV can be recognized by mAb 3F9, due to the 111DCREY115 is conserved in both genotype virus. Meanwhile, this epitope, localized at the
Current diagnostic instruments to find out an infection with the helminth parasite Onchocerca volvulus have restricted efficiency traits. In earlier research, a proteome-wide display screen was performed to establish linear epitopes on this parasites proteome, leading to the discovery of 1110 antigenic peptide fragments. Here, we investigated three of these peptides utilizing peptide ELISAs and evaluated their sensitivity and specificity. Epitope mapping was carried out, and peptides had been constructed that contained solely the minimal epitope, flanked by a linker. Investigation of the efficiency of these minimal epitope peptides demonstrated that every one three of them have a specificity (as outlined by lack of response in non-helminth-infected people) of 100%, low cross-reactivity (5.6%, 5.6%, and 9.3%, respectively), however low sensitivity (36.9%, 46.5%, and 41.2%, respectively).. Some cross-reactivity was noticed in samples from people contaminated with soil-transmitted helminths or ...
Advances in the field of T cell immunology have contributed to the understanding that cross-reactivity is an intrinsic characteristic of the T cell receptor (TCR), and that each TCR can potentially interact with many different T cell epitopes. To better define the potential for TCR cross-reactivity between epitopes derived from the human genome, the human microbiome, and human pathogens, we developed a new immunoinformatics tool, JanusMatrix, that represents an extension of the validated T cell epitope mapping tool, EpiMatrix. Initial explorations, summarized in this synopsis, have uncovered what appear to be important differences in the TCR cross-reactivity of selected regulatory and effector T cell epitopes with other epitopes in the human genome, human microbiome, and selected human pathogens. In addition to exploring the T cell epitope relationships between human self, commensal and pathogen, JanusMatrix may also be useful to explore some aspects of heterologous immunity and to examine T cell
Conventional methods for locating the epitope of an antibody on an antigen all require amino acid sequencing at some stage of the protocol. The protein footprinting approach, for example, employs...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Of 17 evaluable patients, five developed specific anti-vaccine antibodies, and eight developed anti-Fab T-cell responses. T-cell reactivity was independent of the cellular immune status and was idiotype specific as shown by statistical regression analysis (P = 0.0024) and epitope mapping studies. Intradermal administration of uncoupled recombinant idiotype with appropriate adjuvants may overcome profound clinical immunosuppression and induce specific immune responses. ...
Computational methods provide approaches to identify epitopes in protein Ags to help characterizing potential biomarkers identified by high-throughput genomic or proteomic experiments. PEPOP version 1.0 was developed as an antigenic or immunogenic peptide prediction tool. We have now improved this tool by implementing 32 new methods (PEPOP version 2.0) to guide the choice of peptides that mimic discontinuous epitopes and thus potentially able to replace the cognate protein Ag in its interaction with an Ab. In the present work, we describe these new methods and the benchmarking of their performances. Benchmarking was carried out by comparing the peptides predicted by the different methods and the corresponding epitopes determined by X-ray crystallography in a dataset of 75 Ag-Ab complexes. The Sensitivity (Se) and Positive Predictive Value (PPV) parameters were used to assess the performance of these methods. The results were compared to that of peptides obtained either by chance or by using the
Free resource for searching and exporting immune epitopes. Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data.
Header - mouse anti-human CD227 antibody (BD Biosciences, Clone HMPV) in complex with its highlighted epitope sequence PD(T)RP. ...
Sigma-Aldrich offers abstracts and full-text articles by [Naphatsawan Boonsathorn, Sumolrat Panthong, Sarawut Koksunan, Malinee Chittaganpitch, Siripaporn Phuygun, Sunthareeya Waicharoen, Apichai Prachasupap, Tadahiro Sasaki, Ritsuko Kubota-Koketsu, Mayo Yasugi, Ken-Ichiro Ono, Yasuha Arai, Takeshi Kurosu, Pathom Sawanpanyalert, Kazuyoshi Ikuta, Yohei Watanabe].
... provides a collection of methods from computational immunology for the development of novel epitope-based vaccines including HLA ligand or potential T-Cell epitope prediction, an epitope selection framework for vaccine design, and a method to design optimal string-of-beads vaccines. Additionally, EpiToolKit provides several other tools ranging from HLA typing based on NGS data, to prediction of polymorphic peptides ...
Antibodies, Hiv, Hiv-1, Immunization, Neutralizing Antibodies, Rabbits, Glycoprotein, Animals, Proteins, Vaccines, Epitopes, Humoral Immune Responses, Identification, Virus, Water, Antibody Responses, Epitope Mapping, Neutralizing Antibody, Strains, Subunit Vaccines
Khavrutskii, I. V., S. Chaudhury, S. M. Stronsky, D. W. Lee, J. G. Benko, A. Wallqvist, S. Bavari, and C. L. Cooper. Quantitative analysis of repertoire-scale immunoglobulin properties in vaccine-induced B-cell responses. Frontiers in Immunology. 2017 August 14; 8:910. [PDF]. Chaudhury, S., G. D. Gromowski, D. R. Ripoll, I. V. Khavrutskii, V. Desai, and A. Wallqvist. Dengue virus antibody database: systematically linking serotype-specificity with epitope mapping in dengue virus. PLOS Neglected Tropical Diseases. 2017 February 21; 11(2):e0005395. [PDF]. Lee, D. W., I. V. Khavrutskii, A. Wallqvist, S. Bavari, C. L. Cooper, and S. Chaudhury. BRILIA: integrated tool for high-throughput annotation and lineage tree assembly of B-cell repertoires. Frontiers in Immunology. 2017 January 17; 7:681. [PDF]. ...
Large variety of antigen-specific SARS-CoV-2 Peptide Pools, SARS-CoV-2 Peptide Microarray & COVID-19 Epitope Mapping Sets comprising SARS-CoV-2 Epitopes.
Pepscan is a provider of peptide based products & services. High quality custom peptides services, meeting the needs of life science researchers worldwide.
Epitope and STC Technologies, two firms concentrating in specialized oral fluids and related devices for diagnostic testing, expect to announce a stock merger valued at roughly $200 million.
Epitope prediction based on random peptide library screening has become a focus as a promising method in immunoinformatics research. Some novel software and web-based servers have been proposed in recent years and have succeeded in given test cases. However, since the number of available mimotopes with the relevant structure of template-target complex is limited, a systematic evaluation of these methods is still absent. In this study, a new benchmark dataset was defined. Using this benchmark dataset and a representative dataset, five examples of the most popular epitope prediction software products which are based on random peptide library screening have been evaluated. Using the benchmark dataset, in no method did performance exceed a 0.42 precision and 0.37 sensitivity, and the MCC scores suggest that the epitope prediction results of these software programs are greater than random prediction about 0.09-0.13; while using the representative dataset, most of the values of these performance measures are
Immunogenic regions of the gp41 transmembrane protein of human immunodeficiency virus type 1 (HIV-1) were previously mapped by examining polyclonal sera from HIV-infected patients and rodent polyclonal and monoclonal antibodies (MAbs) to peptides of gp41. To define the epitopes within these regions to which infected humans respond during the course of infection, the specificity of human MAbs to these regions had to be studied. Using 10 human MAbs identified initially by their reactivity to whole gp41 in HIV-1 lysates, the epitopes within the immunodominant region of gp41 and within a second immunogenic region of gp41 have been mapped. Thus, five MAbs (from five different patients) to the immunodominant domain of gp41 in the vicinity of the cysteines at positions 598 and 604 (hereinafter designated cluster I) reacted with a stretch of 11 amino acids from positions 590 to 600. Four of these five MAbs were reactive with linear epitopes, while one MAb required the conformation conferred by the ...
Highlights: • A human monoclonal antibody against influenza virus was produced from a volunteer. • The antibody was generated from the PBMCs of the volunteer using the fusion method. • The antibody neutralized heterosubtypically group 1 influenza A viruses (H1 and H9). • The antibody targeted a novel epitope in globular head region of the hemagglutinin. • Sequences of the identified epitope are highly conserved among H1 and H9 subtypes. - Abstract: Most neutralizing antibodies elicited during influenza virus infection or by vaccination have a narrow spectrum because they usually target variable epitopes in the globular head region of hemagglutinin (HA). In this study, we describe a human monoclonal antibody (HuMAb), 5D7, that was prepared from the peripheral blood lymphocytes of a vaccinated volunteer using the fusion method. The HuMAb heterosubtypically neutralizes group 1 influenza A viruses, including seasonal H1N1, 2009 pandemic H1N1 (H1N1pdm) and avian H9N2, with a strong ...
Free resource for searching and exporting immune epitopes. Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data.
LifeTein Peptide Library: SARS-CoV-2 Receptor Binding Domain, 4 Fragments [LT-V013] - Overlapping Peptide Library: The SARS-CoV-2 Receptor Binding Domain is divided in to 4 fragments of 20 amino acids per peptide. The resulting overlapping peptide libraries can then be used for processes including continuous and linear epitope mapping, antibody screening and characterization. Reference 1: A highly conserved cryptic epitope in the receptor-binding
JPT Peptide Technologies is a DIN ISO 9001:2015 certified and GCLP compliant integrated provider of innovative peptide based catalog products and custom services.
A panel of 21 alpha-subunit (CD11a) and 10 beta-subunit (CD18) anti-LFA-1 mAbs was screened for ability to activate LFA-1. A single anti-CD11a mAb, MEM-83, was identified which was able to directly induce the binding of T cells to purified ICAM-1 immobilized on plastic. This ICAM-1 binding could be achieved by monovalent Fab fragments of mAb MEM-83 at concentrations equivalent to whole antibody, was associated with appearance of the "activation reporter" epitope detected by mAb 24, and was completely inhibited by anti-ICAM-1 and LFA-1 blocking mAbs. The epitope recognized by mAb MEM-83 was distinct from that recognized by mAb NKI-L16, an anti-CD11a mAb previously reported to induce LFA-1 activation, in that it was constitutively present on freshly isolated peripheral blood mononuclear cells and was not divalent cation dependent for expression. The ICAM-1 binding activity induced by mAb MEM-83 was, however, dependent on the presence of Mg2+ divalent cations. Using an in vitro-translated CD11a ...