0066]Reference herein to microorganism means any prokaryotic organism (e.g. bacteria) or lower eukaryotic organism (e.g. algae, fungi, protozoa). The preferred microorganisms of the present invention are members of the Enterobacteriaceae group of bacteria. The Enterobacteriaceae group comprises, but is not limited to, Enterobacter (e.g. Enterobacter aerogenes, Enterobacter amnigenus, Enterobacter asburiae, Enterobacter cancerogenus, Enterobacter cloacae, Enterobacter cowanii, Enterobacter dissolvens, Enterobacter gergoviae, Enterobacter hormaechei, Enterobacter intermedins, Enterobacter kobei, Enterobacter nimipressuralis, Enterobacter pyrinus, Enterobacter sakazakii, Enterobacter sp., Enterobacter sp. `MS 412`, Enterobacter sp. 16-31, Enterobacter sp. 2002-2301161, Enterobacter sp. 22, Enterobacter sp. 253a, Enterobacter sp. 3-45, Enterobacter sp. 76996, Enterobacter sp. B2/69, Enterobacter sp. B24a, Enterobacter sp. B24b, Enterobacter sp. B41, Enterobacter sp. B509, Enterobacter sp. B5R5, ...
Enterobacter asburiae sp. nov. is a new species that was formerly referred to as Enteric Group 17 and that consists of 71 strains, 70 of which were isolated from humans. Enterobacter asburiae sp. nov. strains gave positive reactions in tests for methyl red, citrate utilization (Simmons and Christensens), urea hydrolysis, L-ornithine decarboxylase, growth in KCN, acid and gas production from D-glucose, and acid production from L-arabinose, cellobiose, glycerol (negative in 1 to 2 days, positive in 3 to 7 days), lactose, D-mannitol, alpha-methyl-D-glucoside, salicin, D-sorbitol, sucrose, trehalose, and D-xylose. They gave negative reactions in the Voges-Proskauer test and in tests for indole, H2S production, phenylalanine, L-lysine decarboxylase, motility, gelatin, utilization of malonate, lipase, DNase, tyrosine clearing, acid production from adonitol, D-arabitol, dulcitol, erythritol, i(myo)-inositol, melibiose, and L-rhamnose. They gave variable reactions in tests for L-arginine dihydrolase ...
The descriptions of Enterobacter taylorae and Enterobacter cancerogenus show differences in key reactions (ornithine decarboxylase and D-sorbitol fermentation) that have not received attention and are inconsistent with the synonymy proposed by Grimont and Ageron (P. A. D. Grimont and E. Ageron, Res. Microbiol. 140:459-465, 1989). A reassessment of the biochemical properties confirms that they are synonymous. We believe that the priority of E. cancerogenus should be maintained in diagnostic and clinical microbiology even if the epithet could be misunderstood in a clinical setting.
The name Enterobacter gergoviae sp. nov. is proposed for a group of organisms isolated from various clinical sources in France, Africa, and the United States and from environmental sources. E. gergoviae deoxyribonucleic acid contains 60% guanine plus cytosine. Deoxyribonucleic acid relatedness studies showed E. gergoviae strains to be very highly related, as is characteristic of strains of a single species. The biochemical profiles of E. gergoviae strains were extremely similar. Phenotypically, E. gergoviae belongs to the family Enterobacteriaceae and is most similar to Enterobacter aerogenes. E. gergoviae is distinguishable from E. aerogenes by its positive urea reaction and negative reactions for KCN, sorbitol, mucate, and gelatinase. Strain CIP 76.01 (= ATCC 33028 = CDC 604-77) is the type strain of this new species.
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Enterobacter asburiae.
Many studies have been performed to determine the interaction between bacterial species and cancer. However, there has been no attempts to demonstrate a possible relationship between Enterobacter spp. and colon cancer so far. Therefore, in the present study, it is aimed to investigate the effects of Enterobacter strains on colon cancer. Bacterial proteins were isolated from 11 Enterobacter spp., one Morganella morganii, and one Escherichia coli strains, and applied onto NCM460 (Incell) and CRL1790 (ATCC) cell lines. Cell viability and proliferation were determined in MTS assay. Flow Cytometry was used to detect CD24 level and apoptosis. Real-Time PCR studies were performed to determine NFKB and Bcl2 expression. Graphpad Software was used for statistical analysis. The results showed that proteins, isolated from the Enterobacter spp., have significantly increased cell viability and proliferation, while decreasing the apoptosis of the cell lines tested. The data in the present study indicated that ...
Background. Enterobacter species have become increasingly important nosocomial pathogens. However, resistance to cephalosporins often complicates the treatment of Enterobacter infection. This study was conducted to evaluate the predictors of mortality and the impact of cephalosporin resistance on outcome in patients with Enterobacter bacteremia.. Methods. A total of 183 patients with Enterobacter bacteremia were retrospectively analyzed. Broad-spectrum cephalosporin resistance was defined as in vitro resistance to cefotaxime or ceftazidime. The main outcome measure was the 30-day mortality rate.. Results. Of 183 patients, 86 (47%) had bacteremia caused by broad-spectrum cephalosporin-resistant Enterobacter species, and their infections were classified as resistant. The 30-day mortality rate of patients with resistant infections (the resistant group) was significantly higher than that of patients with susceptible infections (the susceptible group) (33.7% vs. 18.6%; P = .021). When the 30-day ...
In this study of the diversity of AmpC beta-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC beta-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA DNA hybridization. The genetic environment of the bla(ACT-1) gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the bla(ACT-1), gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated bla(ACT-1) gene originated from the chromosome of E. hormaechei ...
Abstract: This study provides Enterobacter spp. lactose negative strains characterization susceptible of being wrongly identified as Salmonella spp. having into account its meaning for what concerns on food safety. More than five hundred feed samples for farm animal feeding were microbiologically analyzed according the Spanish Normative for feed, in which different media are accepted for detecting Salmonella spp. after performing preenrichement steps. In this study SS agar was chosen for differential isolation. Results show that 55% of those colonies supposed to be Salmonella spp. belonged to the genus Enterobacter when performing confirmation by means of API20E method and TSI/LIA tests, indicating that several strains of the genus Enterobacter have lost their condition of lactose positive microorganisms and becoming lactose negative, inducing confusion when trying to identify Salmonella spp. by traditional and reference methods. ...
Knowledge of the genus Enterobacter and its role in human disease has expanded exponentially in recent years. The incidence of infection in the hospital and the community has increased. New clinical syndromes have been recognized. Enterobacter spp. have also been implicated as causes of other syndromes that traditionally have been associated almost exclusively with more easily treatable pathogens, such as group A streptococci and staphylococci. Rapid emergence of multiple-drug resistance has been documented in individual patients during therapy and in populations and environments with strong selective pressure from antimicrobial agents, especially the cephalosporins. Therapeutic options for patients infected with multiply resistant strains have become severely limited. Carbapenems or, alternatively, fluoroquinolones are the most predictively active options, although resistance to both classes has been observed on rare occasions. Enterobacter spp. appear well adapted for survival and even ...
Shafeeq S, Wang X, Lünsdorf H, Brauner A, Römling U Microorganisms 8 (4) 522 [2020-04-05; online 2020-04-05] :Enterobacter ludwigii is a fermentative Gram-negative environmental species and accidental human pathogen that belongs to the Enterobacter cloacae complex with the general characteristics of the genus Enterobacter. The clinical isolate E. ludwigii CEB04 was derived from a urinary tract catheter of an individual not suffering from catheter-associated urinary tract infection. The draft genome sequence of the high biofilm forming E. ludwigii CEB04 was determined by PacBio sequencing. The chromosome of E. ludwigii CEB04 is comprised of one contig of 4,892,375 bps containing 4596 predicted protein-coding genes and 120 noncoding RNAs. E. ludwigii CEB04 harbors several antimicrobial resistance markers and has an extended cyclic-di-GMP signaling network compared to Escherichia coli K-12. Bioinformatics Compute and Storage [Service] NGI Stockholm (Genomics Applications) [Service] NGI Stockholm ...
This study provides Enterobacter spp. lactose negative strains characterization susceptible of being wrongly identified as Salmonella spp. having into account its meaning for what concerns on food safety. More than five hundred feed samples for farm animal feeding were microbiologically analyzed according the Spanish Normative for feed, in which different media are accepted for detecting Salmonella spp. after performing preenrichement steps. In this study SS agar was chosen for differential isolation. Results show that 55% of those colonies supposed to be Salmonella spp. belonged to the genus Enterobacter when performing confirmation by means of API20E method and TSI/LIA tests, indicating that several strains of the genus Enterobacter have lost their condition of lactose positive microorganisms and becoming lactose negative, inducing confusion when trying to identify Salmonella spp. by traditional and reference methods ...
Microbial ferulic acid decarboxylase (FADase) catalyzes the transformation of ferulic acid to 4-hydroxy-3-methoxystyrene (4-vinylguaiacol) via non-oxidative decarboxylation. Here we report the crystal structures of the Enterobacter sp. Px6-4 FADase and the enzyme in complex with substrate analogues. Our analyses revealed that FADase possessed a half-opened bottom β-barrel with the catalytic pocket located between the middle of the core β-barrel and the helical bottom. Its structure shared a high degree of similarity with members of the phenolic acid decarboxylase (PAD) superfamily. Structural analysis revealed that FADase catalyzed reactions by an
Enterobacter spp. are the third most common pathogen causing pneumonia and the fifth most common pathogen causing bloodstream and surgical site infections in intensive care facilities in the United States (15). Although ∼25% are resistant to expanded-spectrum cephalosporins, carbapenem-resistant Enterobacter spp. remain very unusual (3). Carbapenem resistance in E. aerogenes has been attributed to overproduction of chromosomal cephalosporinase and loss of porins (1, 4, 5, 16). E. cloacae rarely may acquire class A carbapenem-hydrolyzing β-lactamases, such as NMC-A (9, 11) and IMI-1 (14). The carbapenem-hydrolyzing enzyme KPC-2 has been recovered in isolates of Klebsiella spp. (2, 7, 18) and in an isolate of E. cloacae (A. Hossain, M. J. Ferraro, R. M. Pino, R. B. Dew, E. S. Moland, T. J. Lockhart, K. S. Thomson, R. V. Goering, and N. D. Hanson, Abstr. 43rd Intersci. Conf. Antimicrob. Agents Chemother., abstr. C1-664, 2003). A related enzyme, KPC-3, has been recovered in isolates of K. ...
TY - JOUR. T1 - Molecular epidemiology and antibiotic resistance of Enterobacter spp. from three distinct populations in Grampian, UK. AU - Wagenlehner, F. M.. AU - MacKenzie, Fiona. AU - Forbes, Kenneth James. AU - Gould, Ian M. PY - 2002. Y1 - 2002. N2 - The distribution of Enterobacter spp. within the population of Aberdeen Royal Infirmary was compared with the outpatient population with regard to molecular epidemiology and antibiotic resistance. Enterobacter spp. from 60 patients and one environmental site were characterised as ITU, non ITU and outpatients isolates. Thirty-five percent were blood culture isolates. Cefotaxime resistant strains in the hospital were frequent. Cefotaxime (64%) sensitive isolates were inducible for hyperproduction of Bush group I P-lactamase. Isolates were further investigated by PFGE. Isolates (27%) were clonally related and typed in four clusters. Consecutive isolates were studied in selected patients showing minor genomic changes. One environmental isolate ...
Enterobacter species are becoming increasingly important nosocomial pathogens (29). In the most recent National Nosocomial Infections Study data published,Enterobacter is the third-most-common pathogen recovered from the respiratory tract (16). Data from isolates recovered from intensive care units revealed that this organism was also the fourth-most-common pathogen recovered from surgical wounds, the fifth-most-common pathogen recovered from the urinary tract, and the fifth-most-common pathogen recovered from blood (16). Risk factors for nosocomial Enterobacter infection include the prior use of antimicrobial agents, a prolonged hospital stay, a serious underlying illness, immunosuppression, and the presence of a foreign device (29).. Resistance to expanded-spectrum cephalosporins, broad-spectrum penicillins, and aztreonam usually emerges inEnterobacter spp. due to a mutation in a chromosomal gene, ampD, that normally prevents high-level expression of this organisms chromosomal β-lactamase ...
TY - JOUR. T1 - Structure of the exopolysaccharide produced by Enterobacter amnigenus. AU - Cescutti, Paola. AU - Kallioinen, Anne. AU - Impallomeni, Giuseppe. AU - Toffanin, Renato. AU - Pollesello, Piero. AU - Leisola, Matti. AU - Eerikäinen, Tero. PY - 2005. Y1 - 2005. N2 - The bacterial species Enterobacter amnigenus was isolated from sugar beets harvested in Finland. It produced an exopolysaccharide rich in l-fucose, which gave viscous water solutions. Its primary structure was determined mainly by NMR spectroscopy and ESIMS of oligosaccharides and a polysaccharide with decreased molecular weight, obtained by Smith degradation of the O-deacetylated native polymer.. AB - The bacterial species Enterobacter amnigenus was isolated from sugar beets harvested in Finland. It produced an exopolysaccharide rich in l-fucose, which gave viscous water solutions. Its primary structure was determined mainly by NMR spectroscopy and ESIMS of oligosaccharides and a polysaccharide with decreased molecular ...
The waxworms can digest polyethylene plastic films into ethylene glycol, a compound which biodegrades rapidly. This unusual ability to process matter typically thought of as non-edible may originate with the waxworms ability to process beeswax. Secluded from the guts of Plodia interpunctella wax worms, there are two strains of bacteria, Enterobacter asburiae, and Bacillus sp. Scientists have discovered these two different strains of bacteria are capable of decomposing polyethylene.. Over 12 hours, laboratory tests have confirmed with a polyethylene shopping bag, containing approximately 100 Galleria mellonella that waxworms, were able to consume almost 0.1 gram of the plastic bag.. Plastic eating bugs is an excellent solution to the worlds overwhelming plastic problem. However, Melittologists, bee experts disagree, regarding the dangers of an extinct bee population from the world. The bee is an invaluable species to the ecosystem, and it is essential to fight the ever-growing plastic ...
|span||i|Enterobacter|/i| sp. strain ODB01, which was isolated from the Changqing oil field, can degrade crude oil efficiently and use crude oil as its sole source of carbon and energy. We report the complete genome sequence of ODB01. The results promote its application in the remediation of petroleum con|/span| …
Download Digital illustration of Enterobacter Gram-negative bacilli. #199523642 high-end stock photo. Expensive images and pictures of Digital illustration of Enterobacter Gram-negative bacilli. are authentic and high-quality, brought to you by professional photographers. bacteria, three dimensional
The genus Enterobacter is a member of the ESKAPE group, which contains the major resistant bacterial pathogens. First described in 1960, this group member has proven to be more complex as a result of the exponential evolution of phenotypic and genotypic methods. Today, 22 species belong to the Enterobacter genus. These species are described in the environment and have been reported as opportunistic pathogens in plants... ...
Some symptoms of Enterobacter infections include bacteremia, lower respiratory tract infections, skin infections, soft tissue infections, urinary tract infections, UTI, endocarditis, intraabdominal infections, septic arthritis, osteomyelitis, and ophthalmic infections. They are an opportunistic pathogens that rarely cause disease in otherwise healthy individuals. This bacteriums virulence seems to be due largely to an endotoxin that it produces. Nosocomial infections are the most frequent type of Enterobacter infections, but community-acquired infections are sometimes observed. The bacteria usually infects people who stay in the hospital, especially on the ICU, for long periods of time as well as people how have used many antimicrobial agents, have serious underlying conditions (eg: diabetes, malignancies, burns, mechanical ventilation, etc.), use foreign devices such as intravenous catheters, and immunosuppression.These infections can be contracted endogenously via colonization of the skin, ...
Enterobacter infections can include bacteremia, lower respiratory tract infections, skin and soft-tissue infections, urinary tract infections (UTIs), endocarditis, intra-abdominal infections, septic arthritis, osteomyelitis, CNS infections, and ophthalmic infections. Enterobacter infections can necessitate prolonged hospitalization, multiple ...
Environmental pollution implies any alterations in the surroundings, but it is restricted in use especially to mean any deterioration in the physical, chemical...
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Misc.Comments : Mobilizable expression vector encoding the lacZalpha (lacZ) peptide permitting visual detection of recombinants. Contains priming sites useful for sequencing. Replicates to high copy number in E.coli and Enterobacter aerogenes. Contains the basis of mobilization (bom) and origin of transfer (oriT) sequences from RP4. The order of the major features in this plasmid is:lacZ/MCS - pMB1 ori - kanR - oriT/bom. pARO181 (ATCC 77125) and pARO191 (77126) differ in the orientation of the MCS in lacZ. Can be used as an expression vector in bacteria of the family Enterobacteriaceae, or as a mobilizable suicide vector for site-specific insertional mutagenesis in diverse other bacterial strains. Restriction digests of the clone give the following sizes (kb): HindIII/PvuI--6.0; PvuI--6.0; EcoRI/BglII--3.7,2.3; EcoRI/PvuI--6.0. (ATCC staff) Medium is 1236 LB plus kanamycin ...
Chromosomal ampC genes in Enterobacter species other than Enterobacter cloacae, and ancestral association of the ACT-1 plasmid-encoded cephalosporinase to Enterobacter ...
A total of 50 samples were examined, which consisted of 25 coins and currency notes each. Among the currency screened, 10 coins and 15 notes showed growth of single bacterial species, 11 coins and 9 notes showed the growth of two types of bacterial species each, and three coins and one note had grown three different organisms. The common fungal isolate from two of the coins was Aspergillus niger (n = 2). The bacterial isolates include Staphylococcus aureus (n = 11), Klebsiella spp. (n = 6), Escherichia coli (n = 01), Staphylococcus epidermidis (n = 3), Citrobacter spp. (n = 7), Pseudomonas spp. (n = 4), Acinetobacter spp. (n = 4), Enterobacter agglomerans (n = 2), Micrococcus spp. (n = 1), α - haemolytic Streptococcus spp. (n = 1) and Bacillus spp. (n = 35).. We have observed that 96% of the coins and 100% of the currency notes were found to be contaminated with different bacterial species, of which S. aureus, Klebsiella spp. and E. coli can be considered as pathogens, as they have been ...
1979, Gavini F., Institut National de la Santé et de la Recherche Médicale, Lab. dEcologie et de Physiologie du Système Digestif - CUETM (Enterobacter agglomerans)
Procedures indicated for various Enterobacter infections may include the following: Removal of central venous catheters within 72 hours of gram-negative bacilli infections (This has been shown to low... more
Relationship between intestinal microbial dysbiosis and primary liver cancer [2019] [80000925] [Hepatobiliary & Pancreatic Diseases International] [Journal ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacter; complex; Enterobacter ...
是次 NASA 例行微生物檢查中,首度發現太空站中有抗藥性細菌存在。研究人員從在 2015 年收集到的樣本中,發現五種腸桿菌其中三種基因,均與同一物種的呈抗藥性腸桿菌屬細菌 Enterobacter bugandensis 相似。這三種細菌株有機會對初生嬰兒,或欠缺免疫力的病人患上菌血症、心內膜炎,或初生嬰兒敗血病等。不過,細菌至今未有對太空人造成任何影響。. 儘管如此,由於細菌對多種抗生素呈抗藥性反應,長遠有機會對太空人帶來一定健康威脅。另外,細菌株雖然未發現有致病性,但當中 112 個基因卻與致病株基因相同,而這類基因均與致病現象有關,情況令人憂慮;而研究人員利用電腦模型分析後,估計這些「太空站」細菌株有 79% ...
Deangelis KM, Dhaeseleer P, Chivian D, Fortney JL, Khudyakov J, Simmons B, Woo H, Arkin AP, Davenport K W, Goodwin L et al.. 2011. Complete genome sequence of Enterobacter lignolyticus SCF1.. Stand Genomic Sci. 5(1):69-85. ...
Deangelis KM, Dhaeseleer P, Chivian D, Fortney JL, Khudyakov J, Simmons B, Woo H, Arkin AP, Davenport K W, Goodwin L et al.. 2011. Complete genome sequence of Enterobacter lignolyticus SCF1.. Stand Genomic Sci. 5(1):69-85. ...
Looking for online definition of Enterobacter gergoviae in the Medical Dictionary? Enterobacter gergoviae explanation free. What is Enterobacter gergoviae? Meaning of Enterobacter gergoviae medical term. What does Enterobacter gergoviae mean?
Looking for online definition of Enterobacter agglomerans in the Medical Dictionary? Enterobacter agglomerans explanation free. What is Enterobacter agglomerans? Meaning of Enterobacter agglomerans medical term. What does Enterobacter agglomerans mean?
A chromosomal gene of Enterobacter cloacae encoding an outer membrane protein (OmpX) has been cloned. Overproduction of the OmpX protein decreased the quantity of porins in the outer membrane of the parental strain and of Escherichia coli HB101. The ompX gene was located by insertions of the gamma delta sequence into the recombinant plasmid. The polarity of the gene was determined by in vitro transcription and translation of the gamma delta-containing plasmids. The nucleotide sequence of the ompX gene was elucidated by using both inverted terminal repeats of the gamma delta sequence as starting points for M13 dideoxy sequencing. The gene was found to encode a precursor of the OmpX protein consisting of 172 amino acid residues with a molecular mass of 18.6 kDa. The protein contains an N-terminal signal sequence of 23 amino acid residues. The exact cleavage point was established by sequencing the N-terminal part of the mature protein. The OmpX protein has several characteristics in common with ...
In Escherichia coli, prolipoprotein signal peptidase is encoded by the lsp gene, which is organized into an operon consisting of ileS, lsp, and three open reading frames, designated genes x, orf-149, and orf-316. The Enterobacter aerogenes lsp gene was cloned and expressed in E. coli. The nucleotide sequence of the Enterobacter aerogenes lsp gene and a part of its flanking sequences were determined. A high degree of homology was found between the E. coli ileS-lsp operon and the corresponding genes in Enterobacter aerogenes. Furthermore, the same five genes which constitute an operon in E. coli were found in Enterobacter aerogenes in the same order. ...
Enterobacter cloacae species is responsible for nosocomial outbreaks in vulnerable patients in neonatal intensive care units (NICU). The environment can constitute the reservoir and source of infection in NICUs. Herein we report the impact of preventive measures implemented after an Enterobacter cloacae outbreak inside a NICU. This retrospective study was conducted in one level 3 NICU in Lyon, France, over a 6 year-period (2012-2018). After an outbreak of Enterobacter cloacae infections in hospitalized neonates in 2013, several measures were implemented including intensive biocleaning and education of medical staff. Clinical and microbiological characteristics of infected patients and evolution of colonization/infection with Enterobacter spp. in this NICU were retrieved. Moreover, whole genome sequencing was performed on 6 outbreak strains. Enterobacter spp. was isolated in 469 patients and 30 patients developed an infection including 2 meningitis and 12 fatal cases. Preventive measures and education of
The genus Enterobacter belonging to the family Enterobacteriaceae is polyphyletic based on 16S rRNA gene sequence analysis [1-3]. Recently, eleven species belonging to the genus Enterobacter were transferred into the genus Cronobacter and three novel genera (Lelliottia, Pluralibacter, and Kosakonia) based on multilocus sequence analysis of protein-coding genes, rpoB (RNA polymerase β-subunit gene), gyrB (DNA gyrase subunit B gene), infB (initiation translation factor 2 gene), and atpD (ATP synthase β-subunit gene) [1]. Enterobacter cowanii, E. radicincitans, E. oryzae and E. arachidis were reclassified as Kosakonia cowanii, K. radicincitans, K. oryzae and K. arachidis, respectively [1]. Enterobacter sacchari is a new species named for nitrogen-fixing bacteria in association with sugarcane (Saccharum officinarum L.) [2,4] and has been reclassified as Kosakonia sacchari [3]. K sacchari is able to colonize sugarcane plants, fix N2 in association with sugarcane plants and promote plant growth [4]. ...
Carbon metabolism is the most basic aspect of life. This map presents an overall view of central carbon metabolism, where the number of carbons is shown for each compound denoted by a circle, excluding a cofactor (CoA, CoM, THF, or THMPT) that is replaced by an asterisk. The map contains carbon utilization pathways of glycolysis (map00010), pentose phosphate pathway (map00030), and citrate cycle (map00020), and six known carbon fixation pathways (map00710 and map00720) as well as some pathways of methane metabolism (map00680). The six carbon fixation pathways are: (1) reductive pentose phosphate cycle (Calvin cycle) in plants and cyanobacteria that perform oxygenic photosynthesis, (2) reductive citrate cycle in photosynthetic green sulfur bacteria and some chemolithoautotrophs, (3) 3-hydroxypropionate bi-cycle in photosynthetic green nonsulfur bacteria, two variants of 4-hydroxybutyrate pathways in Crenarchaeota called (4) hydroxypropionate-hydroxybutyrate cycle and (5) ...
Methane is metabolized principally by methanotrophs and methanogens in the global carbon cycle. Methanotrophs consume methane as the only source of carbon, while methanogens produce methane as a metabolic byproduct. Methylotrophs, which are microorganisms that can obtain energy for growth by oxidizing one-carbon compounds, such as methanol and methane, are situated between methanotrophs and methanogens. Methanogens can obtain energy for growth by converting a limited number of substrates to methane under anaerobic conditions. Three types of methanogenic pathways are known: CO2 to methane [MD:M00567], methanol to methane [MD:M00356], and acetate to methane [MD:M00357]. Methanogens use 2-mercaptoethanesulfonate (CoM; coenzyme M) as the terminal methyl carrier in methanogenesis and have four enzymes for CoM biosynthesis [MD:M00358]. Coenzyme B-Coenzyme M heterodisulfide reductase (Hdr), requiring for the final reaction steps of methanogenic pathway, is divided into two types: cytoplasmic HdrABC in ...
Enterobacter aerogenes ATCC ® 15038D-5™ Designation: Genomic DNA from Enterobacter aerogenes strain IFO 12010 TypeStrain=False Application:
Enterobacter aerogenes ATCC ® 15038D-5™ Designation: Genomic DNA from Enterobacter aerogenes strain IFO 12010 TypeStrain=False Application:
TY - JOUR. T1 - Production of adenine arabinoside by gel-entrapped cells of Enterobacter aerogenes in water-organic cosolvent system. AU - Yokozeki, Kenzo. AU - Yamanaka, Shigeru. AU - Utagawa, Takashi. AU - Takinami, Koichi. AU - Hirose, Yoshio. AU - Tanaka, Atsuo. AU - Sonomoto, Kenji. AU - Fukui, Saburo. PY - 1982/12/1. Y1 - 1982/12/1. N2 - Gel-entrapped whole cells of Enterobacter aerogenes, which has a transglycosylation activity, were used to produce adenine arabinoside from uracil arabinoside and adenine, in an appropriate water-organic cosolvent system. Cells of E. aerogenes entrapped with a hydrophilic photo-crosslinkable resin prepolymer, ENT-4000, or a urethane prepolymer, PU-6, had a high and stable transglycosylation activity. To improve the poor solubility in water of the substrate (adenine) and product (adenine arabinoside), dimethyl sulfoxide was selected as the cosolvent based on the criteria of operational stability of the immobilized biocatalyst and solubility of both ...
Enterobacter sakazakii bacteria. Coloured scanning electron micrograph (SEM) of Enterobacter sakazakii bacteria. These are gram-negative rod- shaped bacteria. Enterobacter sp. live in the intestines of humans and animals, as well as soil, water and food. They are mostly opportunistic pathogens, causing diseases such as meningitis and pneumonia. Magnification: x4000 at 35mm size. - Stock Image B220/1054
TY - JOUR. T1 - Effect of crude glycerol-derived inhibitors on ethanol production by Enterobacter aerogenes. AU - Lee, Sang Jun. AU - Kim, Sung Bong. AU - Kang, Seong Woo. AU - Han, Sung Ok. AU - Park, Chulhwan. AU - Kim, Seung Wook. PY - 2012/1/1. Y1 - 2012/1/1. N2 - In this study, ethanol production from pure and crude glycerol using Enterobacter aerogenes ATCC 29007 was evaluated under anaerobic culture conditions. Inhibitory effects of substrate concentrations, pH, and salt concentrations were investigated based on crude glycerol components. Ethanol production was performed with pure glycerol concentrations ranging from 5 to 30 g/L to evaluate the effects of substrate concentration and osmotic pressure. The consumed glycerol was 5-14.33 g/L, and the yield of ethanol was higher than 0.75 mol ethanol/mol glycerol after 24 h of cultivation. To evaluate the inhibitory effects of salts (NaCl and KCl), experiments were performed with 0-20 g/L of each salt. Inhibitory effects of salts were ...
Enterobacter sp. SA187 is an endophytic bacterium that has been isolated from root nodules of the indigenous desert plant Indigofera argentea. SA187 could survive in the rhizosphere as well as in association with different plant species, and was able to provide abiotic stress tolerance to Arabidopsis thaliana. The genome sequence of SA187 was obtained by using Pacific BioScience (PacBio) single-molecule sequencing technology, with average coverage of 275X. The genome of SA187 consists of one single 4,429,597 bp chromosome, with an average 56% GC content and 4,347 predicted protein coding DNA sequences (CDS), 153 ncRNA, 7 rRNA, and 84 tRNA. Functional analysis of the SA187 genome revealed a large number of genes involved in uptake and exchange of nutrients, chemotaxis, mobilization and plant colonization. A high number of genes were also found to be involved in survival, defense against oxidative stress and production of antimicrobial compounds and toxins. Moreover, different metabolic pathways were
Abstract : Enterobacter aerogenes (E. aerogenes) has been commonly described as a versatile opportunistic pathogen in hospital infections. The aim of the present work was to evaluate the impact of biofield treatment on E. aerogenes for its phenotypic and genotypic characteristics. E. aerogenes bearing ATCC 13048 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to Mr. Trivedis biofield treatment and analyzed for antimicrobial susceptibility, minimum inhibitory concentration (MIC), biochemical reactions, and biotype using automated MicroScan Walk-Away® system. In addition, treated group of E. aerogenes was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship of E. aerogenes with different closely related bacterial species. Antimicrobial susceptibility results showed an alteration of 14.28% among ...
Abstract : Enterobacter aerogenes (E. aerogenes) has been commonly described as a versatile opportunistic pathogen in hospital infections. The aim of the present work was to evaluate the impact of biofield treatment on E. aerogenes for its phenotypic and genotypic characteristics. E. aerogenes bearing ATCC 13048 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to Mr. Trivedis biofield treatment and analyzed for antimicrobial susceptibility, minimum inhibitory concentration (MIC), biochemical reactions, and biotype using automated MicroScan Walk-Away® system. In addition, treated group of E. aerogenes was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship of E. aerogenes with different closely related bacterial species. Antimicrobial susceptibility results showed an alteration of 14.28% among ...
Enterobacter aerogenes causes disease in humans through inadvertent bacteria transfer in hospital settings. A selection of enteric bacteria like E. aerogenes are opportunistic and only infect those who already have suppressed host immunity defenses. Infants, the elderly, and those who are in the terminal stages of other disease or are immunosuppressed are prime candidates for such infections (9). Additionally, E. aerogenes as well as other enteric bacteria, is known to have drug-resistant characteristics. There has been some success in dealing with infections through antibiotics, however, the fast development of multidrug resistence has become an increasingly growing problem (3). These multiresistant strains have caused outbreaks in intensive care units (ICUs) in Belgium, France, Austria, and the United States and has further become more emergent than its sister species E. cloacaw (12). Research has shown that E. aerogenes is resistant to ampicillin and it has been more recently discovered that ...
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Enterobacter sakazakii, previously referred to as a yellow-pigmented Enterobacter cloacae was designated as a unique species in 1980. This reclassification was based on differences from E. cloacae in DNA relatedness, pigment production and biochemica
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This assay is only available as part of a panel and cannot be ordered individually.. Enterobacter cloacae is an anaerobic gram-negative nosocomial bacterium that can be acquired through the skin, gastrointestinal tract, urinary tract or derived externally due to the ubiquitous nature. This organism is an opportunistic pathogen, targeting compromised patients such as the young, old, or those that have a severe disease such as human immunodeficiency virus. Enterobacter infections can cause morbidity and mortality and are hard to manage due to multiple antibiotic resistance.. ...
Biochemical Test of Enterobacter aerogenes. They are capsulated, catalase +ve, citrate +ve, flagellated, indole -ve, gram -ve bacteria, etc.
Genus and Species: Enterobacter aerogenes Domain: Prokaryote Optimal Growth Medium: Nutrient Agar Optimal Growth Temperature: 30° C Package: Tube Biosafety Level: 1 Gram Stain: Gram-Negative Shape: Bacillus (rod-shaped)
Genus and Species: Enterobacter aerogenes Domain: Prokaryote Optimal Growth Medium: Nutrient Agar Optimal Growth Temperature: 30° C Package: MicroKwik Culture® Vial Biosafety Level: 1 Gram Stain: Gram-Negative Shape: Bacillus (rod-shaped)
Enterobacteriaceae is a large family of Gram-negative bacteria. It was first proposed by Rahn in 1936, and now includes over 30 genera and more than 100 species. Its classification above the level of family is still a subject of debate, but one classification places it in the order Enterobacterales of the class Gammaproteobacteria in the phylum Proteobacteria.[2][3][4][5] Enterobacteriaceae includes, along with many harmless symbionts, many of the more familiar pathogens, such as Salmonella, Escherichia coli, Klebsiella, and Shigella. Other disease-causing bacteria in this family include Enterobacter and Citrobacter. Members of the Enterobacteriaceae can be trivially referred to as enterobacteria or enteric bacteria,[6] as several members live in the intestines of animals. In fact, the etymology of the family is enterobacterium with the suffix to designate a family (aceae)-not after the genus Enterobacter (which would be Enterobacteraceae)-and the type genus is Escherichia. ...
Abstract Objective: To determine the cause and mode of transmission of a cluster of infections due to Enterobacter cloacae. Design and Setting: Retrospective cohort study in a neonatal intensive-care unit (NICU) from December 1996 to January 1997; environmental and laboratory investigations. Subjects: 60 infants hospitalized in the NICU during the outbreak period. Main Outcome Measures: Odds ratios (OR) linking E cloacae colonization or infection and various exposures. All available E cloacae isolates were typed and characterized by contour-clamped homogenous electric-field electrophoresis to confirm possible cross-transmission. Results: Of eight case-patients, two had bacteremia; one, pneumonia; one, soft-tissue infection; and four, respiratory colonization. Infants weighing |2,000 g and born before week 33 of gestation were more likely to become cases (P|.001). Multivariate analysis indicated that the use of multidose vials was independently associated with E cloacae carriage (OR, 16.3; 95% confidence
In February 1996 we have experienced an outbreak with a multiresistant E aerogenes strain in a 12-bed intensive care unit (ICU) separated in two rooms. Over a 3-day period, four patients were found to be colonized at multiple sites (endotracheal aspirate, urine) by E aerogenes. The medical and nursing charts were reviewed in order to define the sequence of events which led to colonization in these patients. It was found that one patient probably acted as the index-case since he was retrospectively found to be already colonized with E aerogenes in another ward before his admission to the ICU. The three other patients were housed in boxes immediately adjacent to the one of the index case but no strict isolation precautions were taken for these patients since the index case had not been reported as being colonized with a multiresistant organism. All four patients had previously received broad-spectrum antibiotics and three of them were intubated and mechanically ventilated. Colonization ultimately ...
DataMed is a prototype biomedical data search engine. Its goal is to discover data sets across data repositories or data aggregators. In the future it will allow searching outside these boundaries. DataMed supports the NIH-endorsed FAIR principles of Findability, Accessibility, Interoperability and Reusability of datasets with current functionality assisting in finding datasets and providing access information about them.
TY - JOUR. T1 - Prevalence of stably derepressed class I β-lactamase in multiresistant clinical isolates of Enterobacter cloacae in two Hungarian hospitals. AU - Pragai, Zoltan. AU - Csiszár, Károly. AU - Fodor, Eleonóra. AU - Nagy, Elisabeth. PY - 1998/1. Y1 - 1998/1. UR - http://www.scopus.com/inward/record.url?scp=0031974952&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0031974952&partnerID=8YFLogxK. U2 - 10.1111/j.1469-0691.1998.tb00337.x. DO - 10.1111/j.1469-0691.1998.tb00337.x. M3 - Article. AN - SCOPUS:0031974952. VL - 4. SP - 53. EP - 55. JO - Clinical Microbiology and Infection. JF - Clinical Microbiology and Infection. SN - 1198-743X. IS - 1. ER - ...
Fingerprint Dive into the research topics of Structural basis of reduced susceptibility to ceftazidime- avibactam and cefiderocol in enterobacter cloacae due to AmpC R2 loop deletion. Together they form a unique fingerprint. ...
The acquisition of iron is important for the pathogenicity of bacteria and blood. Three different culture environments (Fe stimulation, blood agar plate and normal plate) were used to stimulate Enterobacter cloacae, and their respective pathogenicities were compared at the proteomic, mRNA and metabolomic levels. 2D-DIGE combined with MALDI-TOF-MS/MS, RT-PCR and 1H NMR were used to analyze the differential expression levels of proteins, mRNA and metabolites. A total of 109 proteins were identified by 2D-DIGE and mass spectrometry after pairwise comparison within three culture environments, clustered into 3 classes and 183 functional categories, which were involved in 23 pathways. Based on the 2D-DIGE results, multiple proteins were selected for verification by mRNA expression. These results confirmed that most of the proteins were regulated at the transcriptional level. Thirty-eight metabolites were detected by NMR, which correlated with the differentially expressed proteins under different treatment
On the Approved Lists of Bacterial Names, and on the Approved Lists of Bacterial Names (Amended Edition), this nomenclatural name is incorrectly cited as Enterobacter cloacae (Jordon [sic] 1890) Hormaeche and Edwards 1960. Publication: Euzéby JP. Corrigenda to the Approved Lists of Bacterial Names and to the amended edition of the Approved Lists of Bacterial Names. Int. J. Syst. Bacteriol. 1997; 47:1271-1272. ...
Enterobacter: This germ is somewhat related to E. coli which you might have heard about. It is found in the GI tract -bowels mostly. When it gets out of the area its supposed to be in, it can cause disease. I think of urinary tract infections, intraabdominal abscesses and sometimes blood stream infections caused from this germ. It is somewhat tough so you might need an ID doc if it gets complicated. ...Read more ...
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Enterobacter sakazakii is an opportunistic pathogen that has been associated with sporadic cases and outbreaks causing meningitis, necrotizing enterocolitis and sepsis especially in neonates. However, up to now little is known about the mechanisms of pathogenicity in E. sakazakii. A necessary state in the successful colonization, establishment and ultimately production of disease by microbial pathogens is the ability to adhere to host surfaces such as mucous membranes, gastric and intestinal epithelial or endothelial tissue. This study examined for the first time the adherence ability of 50 E. sakazakii strains to the two epithelial cell lines HEp-2 and Caco-2, as well as the brain microvascular endothelial cell line HBMEC. Furthermore, the effects of bacterial culture conditions on the adherence behaviour were investigated. An attempt was made to characterize the factors involved in adherence. Two distinctive adherence patterns, a diffuse adhesion and the formation of localized clusters of bacteria on
Enterobacter sakazakii is an opportunistic pathogen that has been associated with sporadic cases and outbreaks causing meningitis, necrotizing enterocolitis and sepsis especially in neonates. However, up to now little is known about the mechanisms of pathogenicity in E. sakazakii. A necessary state in the successful colonization, establishment and ultimately production of disease by microbial pathogens is the ability to adhere to host surfaces such as mucous membranes, gastric and intestinal epithelial or endothelial tissue. This study examined for the first time the adherence ability of 50 E. sakazakii strains to the two epithelial cell lines HEp-2 and Caco-2, as well as the brain microvascular endothelial cell line HBMEC. Furthermore, the effects of bacterial culture conditions on the adherence behaviour were investigated. An attempt was made to characterize the factors involved in adherence. Two distinctive adherence patterns, a diffuse adhesion and the formation of localized clusters of bacteria on
ID ENTCC_3_PE3058 STANDARD; PRT; 338 AA. AC ENTCC_3_PE3058; D5CHN1; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Methylthioribose-1-phosphate isomerase; (ENTCC_3.PE3058). GN OrderedLocusNames=ECL_03090; OS ENTEROBACTER CLOACAE SUBSP. CLOACAE ATCC 13047. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex. OX NCBI_TaxID=716541; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ENTCC_3.PE3058. CC Enterobacter cloacae subsp. cloacae ATCC 13047 chromosome, complete CC genome. CC -!- ANNOTATIONS ORIGIN:D5CHN1_ENTCC CC -!- SIMILARITY: Belongs to the eIF-2B alpha/beta/delta subunits CC family. CC -!- GENE_FAMILY: HOG000224730 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; D5CHN1; -. DR EMBL; CP001918; ADF62626.1; -; Genomic_DNA. DR RefSeq; YP_003613575.1; NC_014121.1. DR ...
Background Enterobacter sakazakii is an emergent pathogen that has been associated with neonatal infections through contaminated powdered infant milk formula. The species was defined by Farmer et al....
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex; Enterobacter cloacae; Enterobacter cloacae subsp. ...
COGEM released a comprehensive database of pathogenicity assessment of around 2575 bacterial species in 2011. The database ranks the pathogenicity of species on a scale of 1 to 4 - 1 being not belonging to a recognized group of disease-invoking agents in humans or animals and having an extended history of safe usage and 4 being a species that can cause a very serious human disease, for which no prophylaxis is known ...
Alterations in the human gut microbiome associated with Helicobacter pylori infection [2019] [80001141] [FEBS Open Bio] [Journal ...
Lipase producing bacteria were screened in enrichment culture medium supplemented with olive oil as a sole source of carbon. Furthermore, methanol (30%, v/v) was also used to acquire the methanol tolerant lipase. The clear area around the colonies on the tributyrin agar plate was evaluated as lipase production. The greatest lipolytic strains were also examined on the olive oil plate complemented with phenol red, as a pH indicator. Results showed this isolate was a strain which displayed the maximum pink area around the colony. The 16S rDNA gene of MG isolate was amplified and sequenced (Genbank Accession No. MF927590.1) and compared by BLAST investigation to other bacteria in the NCBI database. The results proposed a near relationship between MG10 isolate and the other members of the Enterobacter genus with a extreme sequence homology (99%) to Enterobacter cloacae. The phylogenetic tree (Fig. 1) designated that the strain MG10 was associated with Enterobacter species and used for the following ...
Common Names of Plant Diseases...R. Jay Stipes, primary collator (last update 3/8/93) BACTERIAL DISEASES Bacterial wetwood Enterobacter nimipressuralis (Carter) Brenner et al. = Erwinia nimipressuralis Carter Bacillus megaterium de Bary Pseudomonas fluorescens Migula Discoloration (xylem) Bacteria Elm leaf scorch...