0066]Reference herein to "microorganism" means any prokaryotic organism (e.g. bacteria) or lower eukaryotic organism (e.g. algae, fungi, protozoa). The preferred microorganisms of the present invention are members of the Enterobacteriaceae group of bacteria. The Enterobacteriaceae group comprises, but is not limited to, Enterobacter (e.g. Enterobacter aerogenes, Enterobacter amnigenus, Enterobacter asburiae, Enterobacter cancerogenus, Enterobacter cloacae, Enterobacter cowanii, Enterobacter dissolvens, Enterobacter gergoviae, Enterobacter hormaechei, Enterobacter intermedins, Enterobacter kobei, Enterobacter nimipressuralis, Enterobacter pyrinus, Enterobacter sakazakii, Enterobacter sp., Enterobacter sp. `MS 412`, Enterobacter sp. 16-31, Enterobacter sp. 2002-2301161, Enterobacter sp. 22, Enterobacter sp. 253a, Enterobacter sp. 3-45, Enterobacter sp. 76996, Enterobacter sp. B2/69, Enterobacter sp. B24a, Enterobacter sp. B24b, Enterobacter sp. B41, Enterobacter sp. B509, Enterobacter sp. B5R5, ...
The name Enterobacter gergoviae sp. nov. is proposed for a group of organisms isolated from various clinical sources in France, Africa, and the United States and from environmental sources. E. gergoviae deoxyribonucleic acid contains 60% guanine plus cytosine. Deoxyribonucleic acid relatedness studies showed E. gergoviae strains to be very highly related, as is characteristic of strains of a single species. The biochemical profiles of E. gergoviae strains were extremely similar. Phenotypically, E. gergoviae belongs to the family Enterobacteriaceae and is most similar to Enterobacter aerogenes. E. gergoviae is distinguishable from E. aerogenes by its positive urea reaction and negative reactions for KCN, sorbitol, mucate, and gelatinase. Strain CIP 76.01 (= ATCC 33028 = CDC 604-77) is the type strain of this new species.
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Enterobacter asburiae.
Background. Enterobacter species have become increasingly important nosocomial pathogens. However, resistance to cephalosporins often complicates the treatment of Enterobacter infection. This study was conducted to evaluate the predictors of mortality and the impact of cephalosporin resistance on outcome in patients with Enterobacter bacteremia.. Methods. A total of 183 patients with Enterobacter bacteremia were retrospectively analyzed. Broad-spectrum cephalosporin resistance was defined as in vitro resistance to cefotaxime or ceftazidime. The main outcome measure was the 30-day mortality rate.. Results. Of 183 patients, 86 (47%) had bacteremia caused by broad-spectrum cephalosporin-resistant Enterobacter species, and their infections were classified as resistant. The 30-day mortality rate of patients with resistant infections (the resistant group) was significantly higher than that of patients with susceptible infections (the susceptible group) (33.7% vs. 18.6%; P = .021). When the 30-day ...
In this study of the diversity of AmpC beta-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC beta-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA DNA hybridization. The genetic environment of the bla(ACT-1) gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the bla(ACT-1), gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated bla(ACT-1) gene originated from the chromosome of E. hormaechei ...
Enterobacter spp. are the third most common pathogen causing pneumonia and the fifth most common pathogen causing bloodstream and surgical site infections in intensive care facilities in the United States (15). Although ∼25% are resistant to expanded-spectrum cephalosporins, carbapenem-resistant Enterobacter spp. remain very unusual (3). Carbapenem resistance in E. aerogenes has been attributed to overproduction of chromosomal cephalosporinase and loss of porins (1, 4, 5, 16). E. cloacae rarely may acquire class A carbapenem-hydrolyzing β-lactamases, such as NMC-A (9, 11) and IMI-1 (14). The carbapenem-hydrolyzing enzyme KPC-2 has been recovered in isolates of Klebsiella spp. (2, 7, 18) and in an isolate of E. cloacae (A. Hossain, M. J. Ferraro, R. M. Pino, R. B. Dew, E. S. Moland, T. J. Lockhart, K. S. Thomson, R. V. Goering, and N. D. Hanson, Abstr. 43rd Intersci. Conf. Antimicrob. Agents Chemother., abstr. C1-664, 2003). A related enzyme, KPC-3, has been recovered in isolates of K. ...
TY - JOUR. T1 - Molecular epidemiology and antibiotic resistance of Enterobacter spp. from three distinct populations in Grampian, UK. AU - Wagenlehner, F. M.. AU - MacKenzie, Fiona. AU - Forbes, Kenneth James. AU - Gould, Ian M. PY - 2002. Y1 - 2002. N2 - The distribution of Enterobacter spp. within the population of Aberdeen Royal Infirmary was compared with the outpatient population with regard to molecular epidemiology and antibiotic resistance. Enterobacter spp. from 60 patients and one environmental site were characterised as ITU, non ITU and outpatients isolates. Thirty-five percent were blood culture isolates. Cefotaxime resistant strains in the hospital were frequent. Cefotaxime (64%) sensitive isolates were inducible for hyperproduction of Bush group I P-lactamase. Isolates were further investigated by PFGE. Isolates (27%) were clonally related and typed in four clusters. Consecutive isolates were studied in selected patients showing minor genomic changes. One environmental isolate ...
Enterobacter infections can include bacteremia, lower respiratory tract infections, skin and soft-tissue infections, urinary tract infections (UTIs), endocarditis, intra-abdominal infections, septic arthritis, osteomyelitis, CNS infections, and ophthalmic infections. Enterobacter infections can necessitate prolonged hospitalization, multiple ...
Misc.Comments : Mobilizable expression vector encoding the lacZalpha (lacZ) peptide permitting visual detection of recombinants. Contains priming sites useful for sequencing. Replicates to high copy number in E.coli and Enterobacter aerogenes. Contains the basis of mobilization (bom) and origin of transfer (oriT) sequences from RP4. The order of the major features in this plasmid is:lacZ/MCS - pMB1 ori - kanR - oriT/bom. pARO181 (ATCC 77125) and pARO191 (77126) differ in the orientation of the MCS in lacZ. Can be used as an expression vector in bacteria of the family Enterobacteriaceae, or as a mobilizable suicide vector for site-specific insertional mutagenesis in diverse other bacterial strains. Restriction digests of the clone give the following sizes (kb): HindIII/PvuI--6.0; PvuI--6.0; EcoRI/BglII--3.7,2.3; EcoRI/PvuI--6.0. (ATCC staff) Medium is 1236 LB plus kanamycin ...
Chromosomal ampC genes in Enterobacter species other than Enterobacter cloacae, and ancestral association of the ACT-1 plasmid-encoded cephalosporinase to Enterobacter ...
A total of 50 samples were examined, which consisted of 25 coins and currency notes each. Among the currency screened, 10 coins and 15 notes showed growth of single bacterial species, 11 coins and 9 notes showed the growth of two types of bacterial species each, and three coins and one note had grown three different organisms. The common fungal isolate from two of the coins was Aspergillus niger (n = 2). The bacterial isolates include Staphylococcus aureus (n = 11), Klebsiella spp. (n = 6), Escherichia coli (n = 01), Staphylococcus epidermidis (n = 3), Citrobacter spp. (n = 7), Pseudomonas spp. (n = 4), Acinetobacter spp. (n = 4), Enterobacter agglomerans (n = 2), Micrococcus spp. (n = 1), α - haemolytic Streptococcus spp. (n = 1) and Bacillus spp. (n = 35).. We have observed that 96% of the coins and 100% of the currency notes were found to be contaminated with different bacterial species, of which S. aureus, Klebsiella spp. and E. coli can be considered as pathogens, as they have been ...
1979, Gavini F., Institut National de la Santé et de la Recherche Médicale, Lab. dEcologie et de Physiologie du Système Digestif - CUETM (Enterobacter agglomerans) <- G. ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacter; complex; Enterobacter ...
Deangelis KM, Dhaeseleer P, Chivian D, Fortney JL, Khudyakov J, Simmons B, Woo H, Arkin AP, Davenport K W, Goodwin L et al.. 2011. Complete genome sequence of "Enterobacter lignolyticus" SCF1.. Stand Genomic Sci. 5(1):69-85. ...
Looking for online definition of Enterobacter gergoviae in the Medical Dictionary? Enterobacter gergoviae explanation free. What is Enterobacter gergoviae? Meaning of Enterobacter gergoviae medical term. What does Enterobacter gergoviae mean?
Looking for online definition of Enterobacter agglomerans in the Medical Dictionary? Enterobacter agglomerans explanation free. What is Enterobacter agglomerans? Meaning of Enterobacter agglomerans medical term. What does Enterobacter agglomerans mean?
A chromosomal gene of Enterobacter cloacae encoding an outer membrane protein (OmpX) has been cloned. Overproduction of the OmpX protein decreased the quantity of porins in the outer membrane of the parental strain and of Escherichia coli HB101. The ompX gene was located by insertions of the gamma delta sequence into the recombinant plasmid. The polarity of the gene was determined by in vitro transcription and translation of the gamma delta-containing plasmids. The nucleotide sequence of the ompX gene was elucidated by using both inverted terminal repeats of the gamma delta sequence as starting points for M13 dideoxy sequencing. The gene was found to encode a precursor of the OmpX protein consisting of 172 amino acid residues with a molecular mass of 18.6 kDa. The protein contains an N-terminal signal sequence of 23 amino acid residues. The exact cleavage point was established by sequencing the N-terminal part of the mature protein. The OmpX protein has several characteristics in common with ...
The genus Enterobacter belonging to the family Enterobacteriaceae is polyphyletic based on 16S rRNA gene sequence analysis [1-3]. Recently, eleven species belonging to the genus Enterobacter were transferred into the genus Cronobacter and three novel genera (Lelliottia, Pluralibacter, and Kosakonia) based on multilocus sequence analysis of protein-coding genes, rpoB (RNA polymerase β-subunit gene), gyrB (DNA gyrase subunit B gene), infB (initiation translation factor 2 gene), and atpD (ATP synthase β-subunit gene) [1]. Enterobacter cowanii, E. radicincitans, E. oryzae and E. arachidis were reclassified as Kosakonia cowanii, K. radicincitans, K. oryzae and K. arachidis, respectively [1]. Enterobacter sacchari is a new species named for nitrogen-fixing bacteria in association with sugarcane (Saccharum officinarum L.) [2,4] and has been reclassified as Kosakonia sacchari [3]. K sacchari is able to colonize sugarcane plants, fix N2 in association with sugarcane plants and promote plant growth [4]. ...
Carbon metabolism is the most basic aspect of life. This map presents an overall view of central carbon metabolism, where the number of carbons is shown for each compound denoted by a circle, excluding a cofactor (CoA, CoM, THF, or THMPT) that is replaced by an asterisk. The map contains carbon utilization pathways of glycolysis (map00010), pentose phosphate pathway (map00030), and citrate cycle (map00020), and six known carbon fixation pathways (map00710 and map00720) as well as some pathways of methane metabolism (map00680). The six carbon fixation pathways are: (1) reductive pentose phosphate cycle (Calvin cycle) in plants and cyanobacteria that perform oxygenic photosynthesis, (2) reductive citrate cycle in photosynthetic green sulfur bacteria and some chemolithoautotrophs, (3) 3-hydroxypropionate bi-cycle in photosynthetic green nonsulfur bacteria, two variants of 4-hydroxybutyrate pathways in Crenarchaeota called (4) hydroxypropionate-hydroxybutyrate cycle and (5) ...
Methane is metabolized principally by methanotrophs and methanogens in the global carbon cycle. Methanotrophs consume methane as the only source of carbon, while methanogens produce methane as a metabolic byproduct. Methylotrophs, which are microorganisms that can obtain energy for growth by oxidizing one-carbon compounds, such as methanol and methane, are situated between methanotrophs and methanogens. Methanogens can obtain energy for growth by converting a limited number of substrates to methane under anaerobic conditions. Three types of methanogenic pathways are known: CO2 to methane [MD:M00567], methanol to methane [MD:M00356], and acetate to methane [MD:M00357]. Methanogens use 2-mercaptoethanesulfonate (CoM; coenzyme M) as the terminal methyl carrier in methanogenesis and have four enzymes for CoM biosynthesis [MD:M00358]. Coenzyme B-Coenzyme M heterodisulfide reductase (Hdr), requiring for the final reaction steps of methanogenic pathway, is divided into two types: cytoplasmic HdrABC in ...
Enterobacter aerogenes ATCC ® 15038D-5™ Designation: Genomic DNA from Enterobacter aerogenes strain IFO 12010 TypeStrain=False Application:
Enterobacter aerogenes ATCC ® 15038D-5™ Designation: Genomic DNA from Enterobacter aerogenes strain IFO 12010 TypeStrain=False Application:
Enterobacter sakazakii bacteria. Coloured scanning electron micrograph (SEM) of Enterobacter sakazakii bacteria. These are gram-negative rod- shaped bacteria. Enterobacter sp. live in the intestines of humans and animals, as well as soil, water and food. They are mostly opportunistic pathogens, causing diseases such as meningitis and pneumonia. Magnification: x4000 at 35mm size. - Stock Image B220/1054
TY - JOUR. T1 - Effect of crude glycerol-derived inhibitors on ethanol production by Enterobacter aerogenes. AU - Lee, Sang Jun. AU - Kim, Sung Bong. AU - Kang, Seong Woo. AU - Han, Sung Ok. AU - Park, Chulhwan. AU - Kim, Seung Wook. PY - 2012/1/1. Y1 - 2012/1/1. N2 - In this study, ethanol production from pure and crude glycerol using Enterobacter aerogenes ATCC 29007 was evaluated under anaerobic culture conditions. Inhibitory effects of substrate concentrations, pH, and salt concentrations were investigated based on crude glycerol components. Ethanol production was performed with pure glycerol concentrations ranging from 5 to 30 g/L to evaluate the effects of substrate concentration and osmotic pressure. The consumed glycerol was 5-14.33 g/L, and the yield of ethanol was higher than 0.75 mol ethanol/mol glycerol after 24 h of cultivation. To evaluate the inhibitory effects of salts (NaCl and KCl), experiments were performed with 0-20 g/L of each salt. Inhibitory effects of salts were ...
Enterobacter sp. SA187 is an endophytic bacterium that has been isolated from root nodules of the indigenous desert plant Indigofera argentea. SA187 could survive in the rhizosphere as well as in association with different plant species, and was able to provide abiotic stress tolerance to Arabidopsis thaliana. The genome sequence of SA187 was obtained by using Pacific BioScience (PacBio) single-molecule sequencing technology, with average coverage of 275X. The genome of SA187 consists of one single 4,429,597 bp chromosome, with an average 56% GC content and 4,347 predicted protein coding DNA sequences (CDS), 153 ncRNA, 7 rRNA, and 84 tRNA. Functional analysis of the SA187 genome revealed a large number of genes involved in uptake and exchange of nutrients, chemotaxis, mobilization and plant colonization. A high number of genes were also found to be involved in survival, defense against oxidative stress and production of antimicrobial compounds and toxins. Moreover, different metabolic pathways were
Abstract : Enterobacter aerogenes (E. aerogenes) has been commonly described as a versatile opportunistic pathogen in hospital infections. The aim of the present work was to evaluate the impact of biofield treatment on E. aerogenes for its phenotypic and genotypic characteristics. E. aerogenes bearing ATCC 13048 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to Mr. Trivedis biofield treatment and analyzed for antimicrobial susceptibility, minimum inhibitory concentration (MIC), biochemical reactions, and biotype using automated MicroScan Walk-Away® system. In addition, treated group of E. aerogenes was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship of E. aerogenes with different closely related bacterial species. Antimicrobial susceptibility results showed an alteration of 14.28% among ...
Abstract : Enterobacter aerogenes (E. aerogenes) has been commonly described as a versatile opportunistic pathogen in hospital infections. The aim of the present work was to evaluate the impact of biofield treatment on E. aerogenes for its phenotypic and genotypic characteristics. E. aerogenes bearing ATCC 13048 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to Mr. Trivedis biofield treatment and analyzed for antimicrobial susceptibility, minimum inhibitory concentration (MIC), biochemical reactions, and biotype using automated MicroScan Walk-Away® system. In addition, treated group of E. aerogenes was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship of E. aerogenes with different closely related bacterial species. Antimicrobial susceptibility results showed an alteration of 14.28% among ...
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Enterobacter sakazakii, previously referred to as a yellow-pigmented Enterobacter cloacae was designated as a unique species in 1980. This reclassification was based on differences from E. cloacae in DNA relatedness, pigment production and biochemica
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Enterobacteriaceae is a large family of Gram-negative bacteria. It was first proposed by Rahn in 1936, and now includes over 30 genera and more than 100 species. Its classification above the level of family is still a subject of debate, but one classification places it in the order Enterobacterales of the class Gammaproteobacteria in the phylum Proteobacteria.[2][3][4][5] Enterobacteriaceae includes, along with many harmless symbionts, many of the more familiar pathogens, such as Salmonella, Escherichia coli, Klebsiella, and Shigella. Other disease-causing bacteria in this family include Enterobacter and Citrobacter. Members of the Enterobacteriaceae can be trivially referred to as enterobacteria or "enteric bacteria",[6] as several members live in the intestines of animals. In fact, the etymology of the family is enterobacterium with the suffix to designate a family (aceae)-not after the genus Enterobacter (which would be "Enterobacteraceae")-and the type genus is Escherichia. ...
Abstract Objective: To determine the cause and mode of transmission of a cluster of infections due to Enterobacter cloacae. Design and Setting: Retrospective cohort study in a neonatal intensive-care unit (NICU) from December 1996 to January 1997; environmental and laboratory investigations. Subjects: 60 infants hospitalized in the NICU during the outbreak period. Main Outcome Measures: Odds ratios (OR) linking E cloacae colonization or infection and various exposures. All available E cloacae isolates were typed and characterized by contour-clamped homogenous electric-field electrophoresis to confirm possible cross-transmission. Results: Of eight case-patients, two had bacteremia; one, pneumonia; one, soft-tissue infection; and four, respiratory colonization. Infants weighing |2,000 g and born before week 33 of gestation were more likely to become cases (P|.001). Multivariate analysis indicated that the use of multidose vials was independently associated with E cloacae carriage (OR, 16.3; 95% confidence
In February 1996 we have experienced an outbreak with a multiresistant E aerogenes strain in a 12-bed intensive care unit (ICU) separated in two rooms. Over a 3-day period, four patients were found to be colonized at multiple sites (endotracheal aspirate, urine) by E aerogenes. The medical and nursing charts were reviewed in order to define the sequence of events which led to colonization in these patients. It was found that one patient probably acted as the index-case since he was retrospectively found to be already colonized with E aerogenes in another ward before his admission to the ICU. The three other patients were housed in boxes immediately adjacent to the one of the index case but no strict isolation precautions were taken for these patients since the index case had not been reported as being colonized with a multiresistant organism. All four patients had previously received broad-spectrum antibiotics and three of them were intubated and mechanically ventilated. Colonization ultimately ...
TY - JOUR. T1 - Prevalence of stably derepressed class I β-lactamase in multiresistant clinical isolates of Enterobacter cloacae in two Hungarian hospitals. AU - Pragai, Zoltan. AU - Csiszár, Károly. AU - Fodor, Eleonóra. AU - Nagy, Elisabeth. PY - 1998/1. Y1 - 1998/1. UR - http://www.scopus.com/inward/record.url?scp=0031974952&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0031974952&partnerID=8YFLogxK. U2 - 10.1111/j.1469-0691.1998.tb00337.x. DO - 10.1111/j.1469-0691.1998.tb00337.x. M3 - Article. AN - SCOPUS:0031974952. VL - 4. SP - 53. EP - 55. JO - Clinical Microbiology and Infection. JF - Clinical Microbiology and Infection. SN - 1198-743X. IS - 1. ER - ...
Enterobacter: This germ is somewhat related to "E. coli" which you might have heard about. It is found in the GI tract -bowels mostly. When it gets out of the area its supposed to be in, it can cause disease. I think of urinary tract infections, intraabdominal abscesses and sometimes blood stream infections caused from this germ. It is somewhat tough so you might need an ID doc if it gets complicated. ...Read more ...
Enterobacter sakazakii is an opportunistic pathogen that has been associated with sporadic cases and outbreaks causing meningitis, necrotizing enterocolitis and sepsis especially in neonates. However, up to now little is known about the mechanisms of pathogenicity in E. sakazakii. A necessary state in the successful colonization, establishment and ultimately production of disease by microbial pathogens is the ability to adhere to host surfaces such as mucous membranes, gastric and intestinal epithelial or endothelial tissue. This study examined for the first time the adherence ability of 50 E. sakazakii strains to the two epithelial cell lines HEp-2 and Caco-2, as well as the brain microvascular endothelial cell line HBMEC. Furthermore, the effects of bacterial culture conditions on the adherence behaviour were investigated. An attempt was made to characterize the factors involved in adherence. Two distinctive adherence patterns, a diffuse adhesion and the formation of localized clusters of bacteria on
Background Enterobacter sakazakii is an emergent pathogen that has been associated with neonatal infections through contaminated powdered infant milk formula. The species was defined by Farmer et al....
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex; Enterobacter cloacae; Enterobacter cloacae subsp. ...
Lipase producing bacteria were screened in enrichment culture medium supplemented with olive oil as a sole source of carbon. Furthermore, methanol (30%, v/v) was also used to acquire the methanol tolerant lipase. The clear area around the colonies on the tributyrin agar plate was evaluated as lipase production. The greatest lipolytic strains were also examined on the olive oil plate complemented with phenol red, as a pH indicator. Results showed this isolate was a strain which displayed the maximum pink area around the colony. The 16S rDNA gene of MG isolate was amplified and sequenced (Genbank Accession No. MF927590.1) and compared by BLAST investigation to other bacteria in the NCBI database. The results proposed a near relationship between MG10 isolate and the other members of the Enterobacter genus with a extreme sequence homology (99%) to Enterobacter cloacae. The phylogenetic tree (Fig. 1) designated that the strain MG10 was associated with Enterobacter species and used for the following ...
5 kosov - Paketi konturnih celic (2) - kartonske škatle.. Kombinirano zdravilo z antibakterijskim in protivnetnim učinkom, ki se uporablja pri boleznih prostate.. Lomefloksacin je baktericidni agent širokega spektra iz skupine fluorokinolonov. Vpliva na bakterijsko encimsko DNA girazo, ki omogoča nadpovprečno DNA, tvori kompleks s svojo tetramerno (A2B2 girazno podenoto) in moti transkripcijo in replikacijo DNA, kar vodi do smrti mikrobne celice.. Visoko aktivna na področju neprofitnega poslovanja pnevmofila.. Zmerno arabsko aerogeni, Enterobacter agglomerans.. Odporen na zdravilo Streptococcus spp., Pseudomonas cepacia, Ureaplasma urealyticum, Treponema pallidum, Mycoplasma hominis in anaerobne bakterije.. Večina mikroorganizmov lomefloksacin deluje v nizkih koncentracijah (koncentracija, potrebna za zatiranje rasti 90% sevov, običajno ne več kot 1 μg / ml). Odpornost je redka.. Ekstrakt prostate ima organotropni učinek na prostato. Zmanjšuje stopnjo edema, infiltracijo levkocitov ...
Background We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer. Methods Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments. Results By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed blaSHV-12 associated to the insertion sequence, IS26. Conclusion This is the first study demonstrated the occurrence of SHV-12 in Nigeria ...
CIFRI EST21 Enterobacter aerogenes KCTC 2190 salt tolerant library Enterobacter aerogenes KCTC 2190 cDNA similar to Bacterial Salt Tolerant Gene, mRNA ...
Enterobacter-ek, Citrobacter, Klebsiella eta Escherichiarekin batera bakterio koliformeen multzoa osatzen du. Koliformeak hestean bizi diren bakterioak dira, ezaugarri biokimiko amankomunak dituztenak eta garrantzi handikoak ingurugiro-mikrobiologian, kutsadura fekalaren adierazleak baitira.. Enterobacter giza zein animalien hesteetan bizi da, baina egoera librean ere aurki daiteke (uretan, lur-zoruan...) Espezie batzuk oportunistak dira, gaixotasunak sortuz immunitate-sistema ahula dutenengan; hori dela eta, infekzio nosokomial askoren jatorrian dago bakterio hau.. ...
1. Javed M, Ueltzhöffer V, Heinrich M, Siegrist HJ, Wildermuth R, Lorenz F, Neher RA, Willmann M. "Colistin susceptibility test evaluation of multiple-resistance level Pseudomonas aeruginosa isolates generated in a morbidostat device". Journal of Antimicrobial Chemotherapy, accepted. 2. Wendel AF, Meyer S, Deenen R, Köhrer K, Kolbe-Busch S, Pfeffer K, Willmann M, Kaasch AJ, MacKenzie CR. „Long-Term, Low-Frequency Cluster of a German-Imipenemase-1-Producing Enterobacter hormaechei ssp. steigerwaltii ST89 in a Tertiary Care Hospital in Germany." Microbial Drug Resistance, 2018. 3. Górska A, Peter S, Willmann M, Autenrieth I, Schlaberg R, Huson DH. "Dynamics of the human gut phageome during antibiotic treatment." Computational Biology and Chemistry, 2018 4. Peter S, Bezdan D, Oberhettinger P, Vogel W, Dörfel D, Dick J, Marschal M, Liese J, Weidenmaier C, Autenrieth I, Ossowski S, Willmann M. „Whole-genome sequencing enabling the detection of a colistin-resistant hypermutating Citrobacter ...
Gram-negative rod. Incubate at 30°C. In nutrient broth. NOTE: Bacterial and fungal cultures will be sold and shipped to institutions only. Desired Date of Arrival can be entered …
The draft genome of "Enterobacter lignolyticus" SCF1 was generated at the DOE Joint Genome Institute (JGI) using a combination of Illumina [31] and 454 technologies [32]. For this genome we constructed and sequenced an Illumina GAii shotgun library which generated 50,578,565 reads totaling 3,844 Mb, a 454 Titanium standard library which generated 643,713 reads and two paired end 454 libraries with average insert sizes of 12517 +/- 3129 bp kb and 10286 +/- 2571 bp which generated 346,353 reads totaling 339.3 Mb of 454 data. All general aspects of library construction and sequencing performed at the JGI can be found at the JGI website [33]. The initial draft assembly contained 28 contigs in 1 scaffold. The 454 Titanium standard data and the 454 paired end data were assembled together with Newbler, version 2.3. The Newbler consensus sequences were computationally shredded into 2 kb overlapping fake reads (shreds). Illumina sequencing data was assembled with VELVET, version 0.7.63 [34], and the ...
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PIRIFUR® is shown for the treatment of urinary tract diseases and attending indications delivered by gram-negative microorganisms touchy to nalidixic corrosive, including E. coli, Enterobacter sp, Klebsiella sp.. whats more, most Proteus sp. Mexico pharmacy drugs
Vu, H, and H Nikaido. "Role of beta-lactam hydrolysis in the mechanism of resistance of a beta-lactamase-constitutive Enterobacter cloacae strain to expanded-spectrum beta-lactams.." Antimicrobial Agents and Chemotherapy 27.3 (1985): 393-398. Web. 04 July. 2020. ...
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Check out The Kaji Review from WikEM Press, with over 750 emergency medicine study questions to help you ace that next test and keep up with the latest evidence! ...
Moreira, Maria Aparecida S. et al. Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses. Braz. J. Microbiol., June 2009, vol.40, no.2, p.241-247. ISSN 1517- ...
O presente trabalho teve por objetivo estudar o perfil de bactérias isoladas de infecções piogênicas de pacientes do Hospital Santa Casa de Misericórdia de Prudentópolis/PR, para testar a suscetibilidade destas frente a diversos antibióticos. Neste estudo, foram coletadas 39 amostras, das quais foram isoladas 42 cepas bacterianas. Destas, avaliou-se o perfil de suscetibilidade antimicrobianas de 36 cepas usando a técnica de Kirby-Bauer. Das cepas isoladas, foram encontrados valores similares de bactérias Gram-positivas e Gram-negativas (50%). Dentre as Gram-positivas, o gênero Staphylococcus se destacou, encontrado em aproximadamente 72% das amostras, Streptococcus e Enterococcus em 14%. Quanto às bactérias Gram-negativas, a Escherichia coli foi a mais frequente, sendo encontrada em 52% das amostras, seguida dos gêneros Pseudomonas, Salmonella, Proteus e Enterobacter. Analisando os sítios de infecção, observou-se que as infecções mais frequentes foram encontradas nos membros ...
The increasing trend of β-lactam resistance among Enterobacteriaceae is a worldwide threat. Enterobacteriaceae isolates causing intra-abdominal infections (IAI) from the Study for Monitoring Antimicrobial Resistance Trends (SMART) collected in 2008 and 2009 from the Asia-Pacific region were investigated. Detection of extended-spectrum β-lactamases (ESBLs), AmpC β-lactamases, and carbapenemases was performed by multiplex PCR. A total of 699 Enterobacteriaceae isolates with positive genotypic results, included Escherichia coli (n = 443), Klebsiella pneumoniae (n = 187), Enterobacter cloacae (n = 45), Klebsiella oxytoca (n = 9), Citrobacter freundii (n = 5), Proteus mirabilis (n = 3), Enterobacter aerogenes (n = 2), Morganella morganii (n = 2), and one each of Enterobacter asburiae, Proteus vulgaris, and Providencia rettgeri were analyzed. Nearly 20% of these β-lactamase-producing Enterobacteriaceae isolates were from community-associated IAI. CTX-M (588 isolates, including 428 [72.8%] with ...
Enterobacter aerogenes and Enterobacter cloacae complex are the two species of this genus most involved in healthcare-associated infections that are ESBL and carbapenemase producers. This study characterized, phenotypically and genotypically, 51 isolates of E. aerogenes and E. cloacae complex originating from infection or colonization in patients admitted to a public hospital in Recife, Pernambuco, Brazil, by antimicrobial susceptibility profile, analysis of β-lactamase genes (bla TEM, bla SHV, bla CTX-M, bla KPC, bla VIM, bla IMP and bla SPM), PCR and DNA sequencing, plasmid profile and ERIC-PCR. In both species, the genes bla TEM, bla CTX-M and bla KPC were detected. The DNA sequencing confirmed the variants bla TEM-1, bla CTX-M-15 and bla KPC-2 in isolates. More than one gene conferring resistance in the isolates, including the detection of the three previously cited genes in strains isolated from infection sites, was observed. The detection of bla CTX-M was more frequent in isolates from infection
Circadian clocks are fundamental properties of all eukaryotic organisms and at least some prokaryotic organisms. Recent studies in our laboratory have shown that the gastrointestinal system contains a circadian clock that controls many, if not all, aspects of gastrointestinal function. We now report that at least one species of intestinal bacteria, Enterobacter aerogenes, responds to the pineal and gastrointestinal hormone melatonin by an increase in swarming activity. This swarming behavior is expressed rhythmically, with a period of approximately 24 hrs. Transformation of E. aerogenes to express luciferase with a MotA promoter reveals circadian patterns of bioluminescence that are synchronized by melatonin and whose periods are temperature compensated from 26°C to 40°C. Bioinformatics suggest similarities between the E. aerogenes and cyanobacterial clocks, suggesting the circadian clock may have evolved very early in the evolution of life. They also point to a coordination of host circadian ...
CRE trends during 2006-2015 in the VHA recapitulate the epidemic of carbapenem-resistant K. pneumoniae in the United States and indicate that a "second epidemic" of carbapenem-resistant E. cloacae complex appears to be unfolding. In the United States, the predominant carbapenem-resistant K. pneumoniae genotype is sequence type (ST) 258, which is associated with Tn4401, a mobile genetic element containing blaKPC (7). In contrast, the genetic background of carbapenem-resistant E. cloacae complex is not well defined. Analysis of carbapenem-resistant E. cloacae from the US Midwest and New York, NY, demonstrated dissemination of E. cloacae complex ST171 harboring the blaKPC-3 gene (2,3,8). Further analysis demonstrated that ST171 was associated with a Tn4401 variant within a pBK30683-like plasmid; however, various other plasmids in Enterobacter spp. also harbor blaKPC-3 (4). Of note, in a northeastern US hospital, one third of carbapenem-resistant E. cloacae contained carbapenemases and the rest ...
A 5 day old full term male infant admitted with a two day history of fever, irritability, and convulsions was diagnosed as having Enterobacter cloacae septicaemia and meningitis. A computed tomography (CT) brain scan on admission showed cerebral oedema and areas of low density signals near the occipital horns of the lateral ventricles. A repeat CT scan five days after admission (fig 1) revealed the presence of diffuse pneumocephalus. T1 weighted magnetic resonance images of the brain three days later (fig 2), as well as a CT scan two weeks after admission, showed a pronounced interval increase in the collection of intracranial air. The baby died from multiorgan failure 18 days after admission. ...
Definition of Enterobacter cloacae. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
In material collected from Tannoch Loch, Dunbartonshire, a large free-living, hitherto undescribed amoeba was found. Individual specimens vary in length from 420 to 500µ and in width from 70 to 140µ. Viewed over a black background this amoeba looks dense, white, and opaque, while in transmitted light it has a dusky, almost black, hue, due to the presence in the endoplasm of a large number of small crystals uniform in size, slender and pointed at both ends. There is always a uroid at the hinder end surrounded by bits of debris. The one large nucleus is without a karyosome but has masses of chromatin scattered through the nuclear substance. As a rule there is only one large contractile vacuole, but occasionally a few small ones may be seen at the hinder end. A certain number of nutritive spheres are always present. The author considers this amoeba to be a new species and names it Amoeba taylorae.. ...
1OVM: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid
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1GCE: Structure of the extended-spectrum class C beta-lactamase of Enterobacter cloacae GC1, a natural mutant with a tandem tripeptide insertion.
Video articles in JoVE about 3 flanking region include Generation of Marked and Markerless Mutants in Model Cyanobacterial Species, Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning, Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells, Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production, Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA, Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope, Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis, The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker, Linear Amplification Mediated PCR - Localization of Genetic Elements and Characterization of Unknown Flanking DNA, Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and
NIAID recently announced a research funding opportunity to develop and/or produce diagnostics to quickly detect the key bacteria responsible for antibacterial-resistant infections in hospital settings. The request for applications (RFA) will support diagnostics research related to one or more of the following types of bacteria: Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter species, and extra-intestinal pathogenic Eschericihia coli. NIAID expects to fund 10-15 awards for a total of up to $12 million in 2015, and the maximum length of each award is 5 years ...
Statistical analysis of the data from the last 10 months showed a total of 186 cases of sepsis, out of which 86 cases of severe sepsis and septic shock with a very high rate of mortality (76 patients). Risk factors and comorbidities (a high rate of obesity, cardiac diseases, diabetes and immunodeficiency, elder patients (63.6 years old), and so on) with etiology ranging from MDR GNB (P. aeruginosa, A. baumannii, K. pneumoniae, Enterobacter spp) to gram positive cocci, C. difficile, fungal infections, and various viral infections (Influenza v., Parainfluenza, Enteroviruses and even Hantavirus) could explain the difficulties in the management of critically ill patients. ...
Procedure:. The sample is pipetted directly into ready-to-use vials. Between 0.1-1.0 ml can be directly added to a vial (depending upon the desired sensitivity). Coliform test completed in 12 hours. Yeast and Molds in 48 hours.. Results:. Yeast and Molds: 50 different yogurts tested using direct method; all samples that contained yeast or mold detected in system. None of clean samples detected. All inoculated samples detected. Plate count results agreed 100% with the BioLumix vial results.. Coliform: 45 different types of yogurts were tested; none contained coliform by the BioLumix method or the MPN method. Twenty-four Coliform strains (Citrobacter, Enterobacter, Escherichia, and Klebsiella) tested, and all detected in the vial. Twenty-one non-coliform bacteria; one detected, except three Salmonella strains. ...
Methyl Red (MR) test is a biochemical test performed on bacterial species to detect the ability of an organism to produce stable acids end products (Mixed-
An outbreak of multidrug-resistant Enterobacter cloacae infection lasted for 4 months in a neonatal intensive care unit (NICU). Forty-six isolates from the NICU and 20 epidemiologically unrelated strains were characterized by pulsed-field gel electrophoresis (PFGE) and repetitive extragenic palindromic unit b1-primed PCR (REPUb1-PCR) typing. The PFGE patterns after XbaI restriction of the bacterial DNA were analyzed by computer software (Gelcompar) using the UPGMA (unweighted pair group method with arithmetic averages) clustering method and the Dice coefficient. The 46 isolates from the NICU were classified by PFGE typing into five clusters: A (further classified into 7 subtypes, A1 to A7), B, C, D, and E. This outbreak was attributed to multiple genetically related strains of cluster A which had a similarity of 85.8% +/- 4.6%. The minor band differences among strains of cluster A were probably due to minor genetic mutations. The type A1 and A3 strains were isolated from the clinical specimens ...
Other names: ATCC 27155, Bacillus milletiae, Bacterium herbicola, CCUG 539, CFBP 3845, CIP 57.51, DSM 3493, Enterobacter agglomerans, Erwinia herbicola, Erwinia milletiae, ICMP 12534, ICPB 3435, NBRC 102470, NCTC 9381, P. agglomerans, Pantoea agglomerans, Pantoea herbicola, Pseudomonas herbicola ...
Other names: ATCC 27155, Bacillus milletiae, Bacterium herbicola, CCUG 539, CFBP 3845, CIP 57.51, DSM 3493, Enterobacter agglomerans, Erwinia herbicola, Erwinia milletiae, ICMP 12534, ICPB 3435, NBRC 102470, NCTC 9381, P. agglomerans, Pantoea agglomerans, Pantoea herbicola, Pseudomonas herbicola ...
A potential risk for workers exposed to inhalation of endotoxin, as well as the primary tissue changes, is the possibility of subsequent development of adult respiratory distress syndrome. Accordingly, hamsters were administered one hour aerosols of Enterobacter agglomerans and allowed to rest for six hours to produce maximum microlesions in the lung. One hour before this peak, the animals were injected intravascularly with the same suspension used in the aerosol. After one hour the animals were killed and bronchopulmonary lavages were made for analysis of free lung cells. As anaesthesia alone has been reported to be one of the operative procedures that enhance the effect of previous exposure to endotoxin, controls had to include sham anaesthesia with no intravascular injection. Endotoxin inhalation induced significant increases in total number of pulmonary leucocytes, mostly neutrophils, but with a concomitant relative decrease in number of alveolar macrophages. These polymorphonuclear ...
In article ,01HNUX051MGI0027IO at vax.rhodes.edu, HILL at NS.RHODES.EDU writes: ,From: HILL at NS.RHODES.EDU ,Subject: Re: Need help on MR-VP test ,Date: 7 Mar 1995 12:56:41 -0800 ,Pong Nim writes: ,,I am having problem obtaining a positive test for Methy Red (MR) ,,and Voges-Proskauer (VP) reaction. ,,I have grew Enterobacter aerogenes & Serratia marcescens in ,,MR-VP broth at 35 C for 48 hrs. Five drops of MR were added. ,,on one set of MR-VP broth. Twelve drops of VP reagent I and ,,three drops of VP reagent II were added to the second set of ,,MR-VP broth. All reagents were made fresh on the day of the test. ,,Still I cant seem to get any positive reaction at all. What ,,am I doing wrong. Your helpful advice would greatly be appreciate. ,In reply: ,The Enterobacter, of course, should be negative for the MR test -- and while ,some S. marcescens will be positive, not all will be. So probably you just ,have an MR-negative strain of Serratia. Try over with E. coli, and I bet itll ,work. ,As ...
The study evaluated the activity of ceftolozane-tazobactam (C/T) against 94 unique clinical isolates of complex (ECC). No difference was observed according to the ECC cluster. The activity greatly varied depending on the β-lactamase-producing profile: 100%, 67%, and 19% of wild-type, extended-spectrum β-lactamase (ESBL)-producing, and AmpC-overproducing strains, respectively, were susceptible to C/T. The use of C/T could be of interest for the treatment of some infections caused by ESBL-producing AmpC-nonoverexpressing ECC isolates.
The exopolysaccharide slime colanic acid has been isolated from representative strains of Escherichia coli, Salmonella typhimurium and Aerobacter cloacae. Analysis showed that each polymer contained glucose, galactose, fucose and glucuronic acid, together with acetate and pyruvate. The molar proportions of these components were 1:1·8:1·9:1:1:1 approximately. On the basis of periodate oxidation of the natural and deacetylated polysaccharide, glucose is proposed as the site of the acetyl groups. The pyruvate is attached to galactose. Three neutral oligosaccharides and ten electrophoretically mobile oligosaccharides were isolated and partially characterized. Four of the fragments were esters of pyruvic acid. Most oligosaccharides were isolated from all three polysaccharide preparations. Three further oligosaccharides were isolated from carboxyl-reduced colanic acid and sodium borotritide was used to label the glucose derived from glucuronic acid in these fragments. One trisaccharide was obtained ...
(2016) Benslama, Boulahrouf. Genomics Data. Enterobacter sp. strain Bisph2 was isolated from a sandy soil from Biskra, Algeria and exhibits glyphosate-degrading activity. Multilocus sequence analysis of the 16S rRNA, rpoB, hsp60, gyrB and dnaJ genes demonstrated that Bisph2 might be a member of a...
A new series of phosphonyl derivatives has been prepared and tested for inhibition of serine (classes A and C) beta-lactamases. The results were compared with those previously acquired with aryl phosphonate monoesters and with alkaline hydrolysis rates. A methyl p-nitrophenyl phosphate monoanion was markedly poorer as an inhibitor of the class C beta-lactamase of Enterobacter cloacae P99 than a comparable p-nitrophenyl phosphonate. Phosphonyl fluorides, thiophenyl esters, N-phenylphosphonamidates and a p-nitrophenyl thionophosphonate were, in general, comparable with p-nitrophenyl phosphonates in inhibitory power. The incorporation of a specific amino side chain led to an increase in the rates of inhibition of around 10(4)-fold. Apparently unresponsive to the addition of the side chain to the enzyme was N-phenyl methylphosphonamidate, where binding of the side chain may interfere with access of the leaving group to a proton which is necessary to active-site phosphonylation and inhibition. ...
Clinical findings a. Symptoms and signs most infections mild priority with online viagra buy shipping but infected will have a limited number of bacteria and destroy the antigen fig. Lancet. Comparison with - lactamase production are enterobacter species, p. Aeruginosa, serratia marcescens, escherichia coli, klebsiella pneumoniae, but significant number of patients, more often in critical care of children with ghd have normal intelligence. Cerebral spinal fluid and protein s deficiency should be performed. Left heart failure with hypervolemia owing to multiple classes. The physician is always required if there is an acute-phase reactantfib-rinogen levels are elevated, with many causes of bradycardia with atropine. The hib conjugate vaccine receipt and guillain-barr syndrome. Larger and major vessels entered. Differential diagnosis obstructive sleep apnea, upper airway obstruction eg, enlarged tonsils, especially if pulsatile, variations in tissue damage. Tee is particularly relevant when the ...
In the last year many works have been conducted to find rapid and simple ways to early detection of water microbial contamination. It is proved that the Electronic Nose (EN) technique can be successfully utilized as a method to detect the presence of some bacteria like cyanobacteria or Escherichia coli or Enterobacter aerogenes, and for the detection of chemical contamination. Most of the waterborne bacteria are well known and no dangerous for human healthy, but are responsible of some of the most problematic odor contamination in potable water. Most of the bacteria commonly found in water supply networks are generally ubiquitous, due to their simple nutritional requirements and ability to utilize many different organic compounds as energy sources. In fact, they are able to reduce sulfur and selenium, leading to the appearance of off-odors usually described as wet cloth, cockles, butane, rubber and rotten eggs. In this work a new EN instrument was applied to monitoring the off-flavor of drinking ...
Accepted name: ribulokinase Reaction: ATP + L(or D)-ribulose = ADP + L(or D)-ribulose 5-phosphate Other name(s): ribulokinase (phosphorylating); L-ribulokinase Systematic name: ATP:L(or D)-ribulose 5-phosphotransferase Comments: Ribitol and L-arabinitol can also act as acceptors. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9030-57-3. References: 1. Burma, D.P. and Horecker, B.L. Pentose fermentation by Lactobacillus plantarum. III. Ribulokinase. J. Biol. Chem. 231 (1958) 1039-1051. 2. Lee, N. and Bendet, I. Crystalline L-ribulokinase from Escherichia coli. J. Biol. Chem. 242 (1967) 2043-2050. [PMID: 5336963] 3. Simpson, F.J., Wolin, M.J. and Wood, W.A. Degradation of L-arabinose by Aerobacter aerogenes. I. A pathway involving phosphorylated intermediates. J. Biol. Chem. 230 (1958) 457-472. ...
Microbiology. I have been working on the antibiotic resistance mechanism and the cell membrane transporter in Pseudomonas aeruginosa and Enterobacter cloacae for eight years. Currently we are focusing on the following studies: 1) Investigating the presence of disinfectant resistant bacteria in clinical settings;2) Analyzing resistance genes in antibiotic resistant Enteric bacteria; 3) Examining the accumulation/efflux of antibiotic in the bacteri. ...
Microbiology. I have been working on the antibiotic resistance mechanism and the cell membrane transporter in Pseudomonas aeruginosa and Enterobacter cloacae for eight years. Currently we are focusing on the following studies: 1) Investigating the presence of disinfectant resistant bacteria in clinical settings;2) Analyzing resistance genes in antibiotic resistant Enteric bacteria; 3) Examining the accumulation/efflux of antibiotic in the bacteri. ...
Escherichia coli, Enterococcus, Klebsiella, Streptococcus, Staphylococcus and Enterobacter are many of the bacilli that often could induce UTI in canine. If bacilli obtain the urethral aperture and transfer up into the extra locations of urinary amplitude and alpha including within, the ambiance on the float can no most effective split sterile. Canine afflicted by bacterial overgrowth while in the float are a lot of suitable to acquaintance affliction and ache though informal urine. Loss of urge for food, fever, abhorrent urine odor or pus/blood in urine is a number of the accepted passion of UTI in canines ...
Resultados: Se analizaron 202 (56.7%) informes de pacientes con periodontitis crónica, 139 (39.1%) de periodontitis agresiva y 15 (4.2%) de individuos periodontalmente sanos. La presencia de microorganismos inusuales de tipo entérico fue 36% y la prevalencia de levaduras 7% en las personas con periodontitis. No se encontraron diferencias significantes entre los tres diagnósticos clínicos con respecto a la presencia de microorganismos entéricos y levaduras. La mayor prevalencia de organismos entéricos correspondió a los géneros Klebsiella, Enterobacter y a bacilos Gram negativos no fermentadores. Se encontraron asociaciones estadísticamente significativas entre la presencia o ausencia de microorganismos infrecuentes con la de algunos microorganismos periodontopáticos en los individuos con enfermedad periodontal ...
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Background: Silver-based products have been marketed as an alternative to antibiotics, and their consumption has increased. Bacteria may, however, develop resistance to silver. Aim: To study the presence of genes encoding silver resistance (silE, silP, silS) over time in three clinically important Enterobacteriaceae genera. Methods: Using polymerase chain reaction (PCR), 752 bloodstream isolates from the years 1990-2010 were investigated. Age, gender, and ward of patients were registered, and the susceptibility to antibiotics and silver nitrate was tested. Clonality and single nucleotide polymorphism were assessed with repetitive element sequence-based PCR, multi-locus sequence typing, and whole-genome sequencing. Findings: Genes encoding silver resistance were detected most frequently in Enterobacter spp. (48%), followed by Klebsiella spp. (41%) and Escherichia coli 4%. Phenotypical resistance to silver nitrate was found in Enterobacter (13%) and Klebsiella (3%) isolates. The lowest carriage ...
Discussion. Pantoea agglomerans, previously known as Enterobacter agglomerans, is a Gram-negative bacterium from the Enterobacteriaceae family. It is mainly a plant epiphyte commonly found on plant material and in soil, but it has been reported as an opportunistic pathogen in humans.2,3 Destructive bone lesions after direct penetrating injuries have been described. It is an uncommon cause, with only 31 cases found in the literature (Pantoea was however not isolated in all 31 cases).4,5 Durr et al. published a case report of a destructive lesion in the first metatarsal bone. It was thought to be a tumour, but on biopsy a 2 cm thorn was found within the lesion. The cortex of the metatarsal was destroyed and replaced with granulation tissue. Histology revealed chronic granulation and Pantoea agglomerans was isolated in cultures.4. Further reports of ten cases of osteomyelitis were found: one case after an open fracture, eight cases associated with penetrating injuries without fractures and one case ...
Brown apical necrosis (BAN) of walnut (Juglans regia L.) causes premature fruit drop and yield losses and has been reported to be an important walnut production problem in Spain, Italy, France, and Turkey (1,2). A number of organisms have been associated with BAN on walnut: Xanthomonas arboricola pv. juglandis, Fusarium spp., and Alternaria spp. (3). Since the spring of 2007, BAN was observed in 50 to 60% of the trees in walnut orchards in Taian City and Laiwu City, Shandong Province, China. Surface-disinfested tissue from premature walnut fruits was placed onto potato dextrose agar. Alternaria spp., X. arboricola pv. juglandis, and Pantoea agglomerans (formerly Enterobacter agglomerans) were isolated 76, 35, and 45% of the time, respectively. The P. agglomerans cultures formed a yellow lawn and were rod shaped with the body length of 1.5 to 3.0 μm, width of 0.5 to 1.0 μm, and four to six flagella. In biochemical tests, these bacteria were gram negative, lactose positive, and indole negative. ...
The microbiological quality of water from a wastewater treatment plant that uses sodium hypochlorite as a disinfectant was assessed. Mesophilic aerobic bacteria were not removed efficiently. This fact allowed for the isolation of several bacterial strains from the effluents. Molecular identification indicated that the strains were related to Aeromonas hydrophila, Escherichia coli (three strains), Enterobacter cloacae, Kluyvera cryocrescens (three strains), Kluyvera intermedia, Citrobacter freundii (two strains), Bacillus sp. and Enterobacter sp. The first five strains, which were isolated from the non-chlorinated effluent, were used to test resistance to chlorine disinfection using three sets of variables: disinfectant concentration (8, 20 and 30 mg·L−1), contact time (0, 15 and 30 min) and water temperature (20, 25 and 30 °C). The results demonstrated that the strains have independent responses to experimental conditions and that the most efficient treatment was an 8 mg·L−1 dose of disinfectant
In this study we analysed the spectrum of Enterobacteriaceae in tracheal aspirates of intubated PICU patients from 2005 to 2014. The spectrum of Enterobacteriaceae in lower respiratory tract material revealed Enterobacter spp., E.coli and Klebsiella spp. as the most common isolates (86%). Comparable data of matching study settings are scarce in the current literature. Wilson et al. [5] collected daily tracheal aspirates from intubated children. Consistent with our findings, the most common Gram-negative organisms isolated were Klebsiella spp. and E.coli, followed by Citrobacter freundii and Enterobacter cloacae. Lee et al. [34] described the microbiological spectrum and susceptibility pattern of clinical isolates from a PICU and found a rate of 20% ESBL-positive Klebsiella in 2005. Our study displayed a lower rate with only 6.5% of Klebsiella spp. isolates being ESBL-positive. However, more than half of E.coli isolates (55%), about a quarter of all Klebsiella spp. (28%) isolates and 4 out of 6 ...
The previously described facultatively anaerobic microorganismEnterobacter sp. DG-6, which transforms methoxylated and hydroxylated monoaromatic compounds both aerobically and anaerobically, has been further investigated. The moles percent guanine plus cytosine in DNA has been determined. Two plasmids have been isolated and studied as related to the capability of the bacterium to degrade 4-hydroxy-3-methoxy-cinnamic acid (ferulic acid). The anaerobic O-demethylation mechanism and the pathway of C1-compound oxidation are discussed.
From December 5, 1996, through January 25, 1997, a total of 10 adult patients at an ambulatory hemodialysis center in Maryland had gram-negative bacterial BSIs. Six BSIs were caused by Enterobacter cloacae, four by Pseudomonas aeruginosa, and two by Escherichia coli; two were polymicrobial BSIs. All patients at the hemodialysis center were dialyzed on CS3 hemodialysis machines that had WHOs. Results of a cohort study of all patients receiving dialysis at the center during the 2-month epidemic period indicated that the risk for gram-negative BSI was associated with exposure to any of three particular dialysis machines (seven BSIs in 20 patients who were exposed to one or more of the three machines versus three BSIs in 64 patients who were exposed to the other machines; relative risk=7.5; 95% confidence interval=2.1-26.2). Incompetent valves on WHO waste drain lines were present in eight of 26 dialysis machines and in two of the three implicated machines. Enterobacter cloacae was recovered from ...
INTRODUCTION: Respiratory tract infections are severe and most common types of infection treated by medical practitioners all over the world. Nosocomial pneumonia is the second most common infection, causing high morbidity and mortality and about 80% of nosocomial infections caused by Klebsiella pneumoniae are due to multidrug-resistant strains.. The emergence of antibiotic-resistant bacterial strains necessitates the exploration of alternative antibacterial therapies 1. Important causes of Gram-negative resistance includes extended-spectrum β-lactamases (ESBLs) in Klebsiella pneumoniae, high level third-generation cephalosporin (Amp C) β-lactamase resistance among Enterobacter species observed in pneumonia.. Recent data suggest that because of ESBLs and high-level amp C β-lactamase resistances, use of third-generation cephalosporins may be ineffective in many patients with nosocomial infections 2. EDTA is a polyamino carboxylic acid, a colorless water-soluble metallo-chelator and is known to ...
Previous research by Pollard shows the bactericidal action of Tergitol 7 against gram-positive bacteria. (11) Chapman later developed the medium for the selective isolation of Escherichia coli and other members of the coliform group. According to Chapmans published formula, a medium containing Tergitol 7, proteose peptone no. 3, yeast extract, lactose, and bromothymol blue permitted the unrestricted development of coliform bacteria, while inhibiting the growth of gram-negative spore-formers, gram-positive microorganisms and the swarming of Proteus spp. (1,2) He found the difference between E. coli and Enterobacter aerogenes and other coliforms to be distinct on this medium: E. coli produces yellow colonies with yellow zones and occasionally rust colored centers; E. aerogenes produces greenish-yellow colonies, while non-lactose fermenting microorganisms produce dark red colonies with bluish zones. In addition, Chapman proposed that counts of coliform organisms on Tergitol 7 Agar were found to be ...
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Lecture Exam 4/Final Spring 2016 MW Section- Monday 05/23 @ 8:00am. TTh Section- Tuesday 05/24 @ 8:00am. BIOL 220 IMAGES Enterobacter aerogenes capsule stain Mycobacterium smegmatis\Staphylococcus aureus acid fast st Streptococcus (Enterococcus) faecalis Gram st Gram negative diplococci. Spirillum volutans flagella stain. Office Hours for Spring 2016-M,T,W,Th 7:30 to 8:00 AM. M,W 12:30-2:00 PM. ...
TREATMENT: Renal replacement therapies are required to prevent fatal fluid overload, hyperkalemia, and other uremic complications. These include hemodialysis, peritoneal dialysis, or kidney transplantation. Other therapies include the administration of water-soluble vitamins, phosphate-binding medications, erythropoietin, iron and folic acid (to treat anemia), and bicarbonate buffers (to prevent acidemia). The diet of patients with ESRD is restricted to limited quantities of fluids, and small concentrations of sodium, potassium, and protein, to prevent complications like fluid retention and hyperkalemia. Tight control of blood pressure and blood glucose levels in patients with hypertension or diabetes, respectively, will prolong kidney function and prevent deterioration. ...
A diagnostic test used by hospitals says a recently isolated strain of bacteria is susceptible to the "last resort" antibiotic colistin. But the strain actually ignores treatment with colistin, causing lethal infections in animals.. Through heteroresistance, a genetically identical subpopulation of antibiotic-resistant bacteria can lurk within a crowd of antibiotic-susceptible bacteria. The phenomenon could be causing unexplained treatment failures in the clinic and highlights the need for more sensitive diagnostic tests, researchers say.. In Nature Microbiology (published online Monday, May 9), scientists led by David Weiss, PhD, describe colistin-heteroresistant strains of Enterobacter cloacae, a type of bacteria that has been causing an increasing number of infections in hospitals around the world.. "Heteroresistance has been observed previously and its clinical relevance debated," Weiss says. "We were able to show that it makes a difference in an animal model of infection, and is likely to ...