hepatitis C virus proteinase Cpro-1: cleaves HCV polyprotein p110 to generate N-terminal of p70; enzyme activity is unique HCV characteristic, different from other members of flaviviridiae; amino acid sequence has been determined
Our efforts to study HIV-1 protease structure and function in the current funding period have yielded exciting results that form the basis for a new hypothesis,...
Tobacco Etch Virus (TEV) protease is a cysteine protease that is commonly used to remove fusion tags, including His, GST and MBP, at the N or C termini of recombinant proteins. TEV protease specifically recognizes a seven amino acid sequence Glu-Asn-Leu-T
cysteine proteinase I: cleaves peptides primarily from the C-terminal region of thyroglobulin making thyroid hormone-containing sites accessible to cleavage by other endopeptidases
PD Dr. Thorsten Pfirrmann. I studied Technical Biology from 1995 to 2002 at the University of Stuttgart, Germany, which spawned my keen and longterm interest in the ubiquitin proteasome system. I carried out my Masters (Diploma) work examining the Saccharomyces cerevisiae specific deubiquitinating enzyme Ubp6 in the laboratory of Rohan Baker at the John Curtin School of Medical Research, Canberra, Australia. In 2002, I returned to Stuttgart to join the laboratory of Dieter Wolf, where as a Ph.D. student I discovered a novel conserved E3 ubiquitin ligase complex and experimentally addressed certain aspects of the deubiquitinating enzyme Ubp14. In 2006, I moved to Stockholm, Sweden for postdoctoral studies, and first worked on the function of the Ubiquitin C-terminal hydrolase UCH-L1 in the laboratory of Maria Masucci at Karolinska Institute. In 2008, I joined the laboratory of Per O. Ljungdahl at Stockholm University, where I focused on the ubiquitin proteasome dependent activation mechanism of ...
McCartney, S. A., Brignole, E. J., Kolegraff, K. N., Loveland, A. N., Ussin, L. M., and Gibson, W. Chemical rescue of I-site cleavage in living cells and in vitro discriminates between the cytomegalovirus protease, assemblin, and its precursor, pUL80a. J. Biol. Chem.280:33206-33212 (2005) Loveland, A. N., Chan, C.-K., Brignole, E. J., and Gibson, W. Cleavage of human cytomegalovirus protease pUL80a at internal and cryptic sites is not essential but enhances infectivity. J. Virol. 79:12961-12968 (2005) Wang, J., Loveland, A. N., Kattenhorn, L. M., Ploegh, H. L., and Gibson, W. High-molecular-weight protein (pUL48) of human cytomegalovirus is a competent deubiquitinating protease: Mutant viruses altered in its active-site cysteine or histidine are viable. J. Virol. 80:6003-6012 (2006). Margulies, B. J. and Gibson, W. The chemokine receptor homologue encoded by UL27 of human cytomegalovirus is heavily glycosylated and is present in infected human foreskin fibroblasts and enveloped virus particles. ...
Allcosmeticsource.com Alkaline Protease 100000u/g,1kg/bag,free shipping [EP170508005]- Alkaline Protease 100000u/g,1kg/bag,free shipping What is Alkaline Protease 100000u/g? The Alkaline Protease is a solid enzyme produced by submerged fermentation from Bacillus licheniformis according to the specification of GB/T23527-2009 (China). It is widely applied in detergent, leather depilation and softening, silk degumming, Heparin Sodium, Chondroitin and etc. Function of Alkaline Protease 100000u/g 1. Improve metabolic energy utilization
1GO8: Crystal Structure of a Complex between Pseudomonas Aeruginosa Alkaline Protease and its Cognate Inhibitor: Inhibition by a Zinc-NH2 Coordinative Bond
These intramembrane proteases differ from soluble proteases in a variety of aspects. They are composed of a number of transmembrane domains which harbor the
This study has demonstrated that LHN/A, BoNT/A which has been enzymatically treated to remove its native cell receptor binding domain, can be chemically derivatized without loss of endopeptidase activity, conjugated to a second protein (WGA) to form a stable, soluble conjugate, and delivered to a variety of cells in vitro by a ligand-dependent process to inhibit secretion in a dose-dependent manner via a mechanism involving endopeptidase-dependent cleavage of the natural BoNT/A substrate. These data clearly demonstrate that a hybrid protein has been created by chemically conjugating WGA and LHN/A and that this conjugate is biologically active. Replacement of the cell-binding moiety of a number of toxins has been reported previously (4, 27), but this work represents the first reported replacement of the BoNT cell-binding domain to result in the creation of a functional hybrid.. We have demonstrated internalization of functional endopeptidase into three different neuronal cell culture models. PC12 ...
1PJP: The 2.2 A crystal structure of human chymase in complex with succinyl-Ala-Ala-Pro-Phe-chloromethylketone: structural explanation for its dipeptidyl carboxypeptidase specificity.
... - Instruments Consumables Reagents Advanced BioMatrix,RANDOX,RANDOX ELISA,Biomedical, biochemical reagents, laboratory supplies, equipment, antibodies, ELISA kits, diagnostic reagents, methods of experimental techniques, general analytical instruments, material testing instruments and equipment, used laboratory equipment, instruments and equipment, life sciences, environmental monitoring equipment , measurement, measuring instruments, rotating wall bioreactor, three-dimensional tissue / stem cell culture system; microcapsule
Proteinase K Lyophilized is ideal for making your own Proteinase K solutions at a concentration suitable for your application in protein degradation
Click on a genes description to view its network relationships with genes known to be involved in "regulation of endopeptidase activity" ...
Gentaur molecular products has all kinds of products like :search , Genomed \ GENOMED Proteinase K _ 200 mg \ GN-PK-200 for more molecular products just contact us
It is a formaldehyde-free, next generation smoothing system that uses the latest advances in science, along with only the finest ingredients, to create a product that delivers the results of a traditional keratin smoothing treatment without the harmful chemicals ...
Existing models of the size and scope of investment activity traditionally assume an infinite pool of ex-ante identical projects, despite the fact that managers
長塚 正晃 , 齋藤 裕 , 白土 なほ子 , 藤原 紹生 , 小塚 和人 , 奥山 大輔 , 千葉 博 , 矢内原 巧 日本産科婦人科學會雜誌 51(9), 777-783, 1999 CiNii 外部リンク 機関リポジトリ 医中誌Web 参考文献23件 被引用文献4件 ...
TY - JOUR. T1 - Expression and characterization of human foamy virus proteinase. AU - Fenyöfalvi, György. AU - Bagossi, Péter. AU - Copeland, Terry D.. AU - Oroszlan, Stephen. AU - Boross, Péter. AU - Tözsér, József. PY - 1999/12/3. Y1 - 1999/12/3. N2 - The human foamy virus proteinase was expressed in fusion with maltose binding protein in Escherichia coli and purified. The specific activity of the fusion protein was similar to that of the processed enzyme. The kinetic constants on foamy virus cleavage site substrates were very low but comparable to those obtained with the gag-encoded avian proteinase on its own substrates. The proteinase showed preference for high ionic strength and a pH optimum of 6.6. None of the tested retroviral cleavage site peptides were substrates, however, some peptides representing cleavage sites in retrotransposons were properly processed by the enzyme. Copyright (C) 1999 Federation of European Biochemical Societies.. AB - The human foamy virus proteinase was ...
The deubiquitinating enzyme USP14 has been identified and biochemically studied, but its role in lung cancer remains to be elucidated. The aim of this study was to evaluate the prognostic significance of USP14 in patients with lung adenocarcinoma and to define its role in lung cancer cell proliferation. USP14 mRNA levels in different non-small cell lung cancer (NSCLC) cell lines were detected by real-time qPCR. USP14 protein levels in surgically resected samples from NSCLC patients, and in NSCLC cell lines, were detected by immunohistochemistry or Western blot. The correlation of USP14 expression with clinical characteristics and prognosis was determined by survival analysis. After silencing USP14, cell proliferation was assessed by MTT assay and the cell cycle was measured by FACS assay. It was found that USP14 expression was upregulated in NSCLC cells, especially in adenocarcinoma cells. Over-expression of USP14 was associated with shorter overall survival of patients. Downregulation of USP14
The IFN immune system comprises type I, II, and III IFNs, signals through the JAK-STAT pathway, and plays central roles in host defense against viral infection. Posttranslational modifications such as ubiquitination regulate diverse molecules in the IFN pathway. To search for the deubiquitinating enzymes (DUBs) involved in the antiviral activity of IFN, we used RNA interference screening to identify a human DUB, ubiquitin-specific protease (USP) 13, whose expression modulates the antiviral activity of IFN-α against dengue virus serotype 2 (DEN-2). The signaling events and anti-DEN-2 activities of IFN-α and IFN-γ were reduced in cells with USP13 knockdown but enhanced with USP13 overexpression. USP13 may regulate STAT1 protein because the protein level and stability of STAT1 were increased with USP13 overexpression. Furthermore, STAT1 ubiquitination was reduced in cells with USP13 overexpression and increased with USP13 knockdown regardless of with or without IFN-α treatment. Thus, USP13 ...
TY - JOUR. T1 - APH1, PEN2, and Nicastrin increase Aβ levels and γ-secretase activity. AU - Marlow, Laura. AU - Canet, Rosa M.. AU - Haugabook, Sharie J.. AU - Hardy, John A.. AU - Lahiri, Debomoy K.. AU - Sambamurti, Kumar. PY - 2003/6/6. Y1 - 2003/6/6. N2 - A major component of the amyloid plaque core in Alzheimers disease (AD) is the 40-42-residue amyloid β peptide (Aβ). Mutations linked to AD such as those in presenilins 1 (PS1) and 2 (PS2) invariably increase the longer Aβ42 species that forms neurotoxic oligomers. It is believed that PS1/2 constitute the catalytic subunit of the γ-secretase responsible for the final step in Aβ biogenesis. Recent genetic studies have identified a number of additional genes encoding APH1a, APH1b, PEN2, and Nicastrin proteins, which are part of the γ-secretase complex with PS1. Further, knockout studies using RNAi showed that these components are essential for γ-secretase activity. However, the nature of γ-secretase and how the aforementioned ...
Cohesin is a highly conserved multisubunit complex that holds sister chromatids together in mitotic cells. At the metaphase to anaphase transition, proteolytic cleavage of the α kleisin subunit (Rad21) by separase causes cohesins dissociation from chromosomes and triggers sister-chromatid disjunction. To investigate cohesins function in postmitotic cells, where it is widely expressed, we have created fruit flies whose Rad21 can be cleaved by TEV protease. Cleavage causes precocious separation of sister chromatids and massive chromosome missegregation in proliferating cells, but not disaggregation of polytene chromosomes in salivary glands. Crucially, cleavage in postmitotic neurons is lethal. In mushroom-body neurons, it causes defects in axon pruning, whereas in cholinergic neurons it causes highly abnormal larval locomotion. These data demonstrate essential roles for cohesin in nondividing cells and also introduce a powerful tool by which to investigate protein function in metazoa. © 2008 Elsevier
Separase löst alle eukaryotischen Anaphasen aus, indem sie kohäsionsvermittelndes Cohesin spaltet. Bis dahin wird diese essentielle Protease durch Securin inhibiert. Separase kann alternativ durch Assoziation mit Cdk1-Cyclin B1 gehemmt werden, aber der entsprechende Komplex ist in der frühen Mitose wenig abundant und kann nicht erklären, warum Securin in Vertebraten entbehrlich ist. Die Proteinphosphatase 2A (PP2A) bindet Separase ebenfalls, aber die physiologische Rolle dieser Interaktion bleibt rätselhaft. Durch die Inaktivierung des spindle assembly checkpoint (SAC) in der Metaphase kann die Ubiquitin-Ligase APC/C die proteasomale Zerstörung von Securin (und Cyclin B1) vermitteln und dadurch Separase aktivieren. Obwohl sie strukturell mit Caspasen verwandt ist, wurde Separase bisher nicht mit der Apoptose in Verbindung gebracht. Stattdessen wurde in zwei Studien eine Rolle der Hefe-Separase bei der Reparatur von DNS-Schäden vorgeschlagen. Die Frage, ob diese nichtkanonische ...
It is clear that intramembrane proteolysis regulates many biological processes. As with other intramembrane proteases such as γ‐secretase/presenilin and SPP (Martoglio and Golde, 2003; Selkoe and Schenk, 2003), rhomboids may turn out to be useful therapeutic targets (Opitz et al, 2002; Urban and Freeman, 2003). The lack of an in vitro assay for rhomboid activity has been a limiting factor in the biochemical analysis of their proteolytic mechanisms. We have now demonstrated rhomboid activity both in detergent‐solubilised membrane extracts and in a highly purified fraction. These two versions of the in vitro assay have different utilities. The activity of multiple rhomboids can be rapidly determined in TX100 membrane extracts; and in purified fractions, precise quantification of relative activities can be measured. Here we have exploited both approaches to show that diverse rhomboids have differential activities on three model substrates, and to compare the activities of a number of purified ...
Next-day shipping cDNA ORF clones derived from UBP13 ubiquitin-specific protease UBP13 available at GenScript, starting from $99.00.
Regulated intramembrane proteolysis is certainly a central mobile practice included in sign membrane layer and transduction proteins turnover. condition of MHCII-containing endosomes, highlighting SPPL2a as a possible medicinal focus on for using up and/or modulating T cells. The concept of intramembrane proteases (I-CLIPs) cleaving within the phospholipid bilayer was originally place forwards structured on digesting of the sterol regulatory elementCbinding proteins (SREBP; Goldstein and Brown, 1997; Kopan and Wolfe, 2004). Generally, I-CLIPs operate as component of a proteolytic series known to as governed intramembrane proteolysis (Split; Lichtenthaler et al., 2011). Intracellular websites (ICDs) of many Split substrates function as signaling elements after their proteolytic discharge as exemplified by the Level path (De Strooper et al., 1999; Freeman and Urban, 2002). Structured on their catalytic middle, serine, metallo, or aspartyl I-CLIPs (Wolfe, 2009) can end up being differentiated. The ...
Ready-to-use arrayed siRNA library in single-use plate sets Just rehydrate, and add cells for RNAi screening of human deubiquitinating enzymes Optimization plates available
The normal function of poly (ADP-ribose) polymerase-1 (PARP-1) is the routine repair of DNA damage by adding poly (ADP ribose) polymers in response to a variety of cellular stresses. Recently, it has become widely appreciated that PARP-1 also participates in diverse physiological and pathological functions from cell survival to several forms of cell death and has been implicated in gene transcription, immune responses, inflammation, learning, memory, synaptic functions, angiogenesis and aging. In the CNS, PARP inhibition attenuates injury in pathologies like cerebral ischemia, trauma and excitotoxicity demonstrating a central role of PARP-1 in these pathologies. PARP-1 is also a preferred substrate for several suicidal proteases and the proteolytic action of suicidal proteases (caspases, calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs)) on PARP-1 produces several specific proteolytic cleavage fragments with different molecular weights. These PARP-1 signature fragments are
Gan, Zhihua et al. Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro. Biol. Res., 2017, vol.50. ISSN 0716- ...
Hansson CA, Frykman S, Farmery MR, Tjernberg LO, Nilsberth C, Pursglove SE, Ito A, Winblad B, Cowburn RF, Thyberg J, Ankarcrona M. Nicastrin, presenilin, APH-1, and PEN-2 form active gamma-secretase complexes in mitochondria ...
Would anyone know structural charactersitics of enzymes catalyzing the hydrolysis of 4-methylumbelliferyl-p-guanidinobenzoate to its product, 4-methylumbelliferone? I am presently working with the identification of a sperm protein possessing this enzymatic capability. zu04516 at uabdpo.dpo.uab.edu ...
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Astacin Like Metallo Endopeptidase (ASTL) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species ...
The invention relates to a detergent composition having a wool compatible high alkaline protease with at least one mutation in each of the regions 96-110 and 123-135 according to the BPN-numbering. The detergent composition is preferably substantially free of bleach and does contain so-called dye transfer inhibition technology.
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Ubiquitin-specific protease that interacts with Bre5p to co-regulate anterograde and retrograde transport between endoplasmic reticulum and Golgi ...
Involved in the breakdown of the scaffold protein during the late stages of assembly of the herpes-virus virion. Inhibited by diisopropyl fluorophosphate. Type example of
Creative Peptides offers Endopeptidase L4 for your research. We also provide custom peptide synthesis, process development, GMP manufacturing.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants ...
Addresses: GOOBARLARSSON L, KAROLINSKA INST, CTR MICROBIOL & TUMORBIOL, BOX 280, S-17177 STOCKHOLM, SWEDEN. UPPSALA UNIV, BMC, DEPT MOLEC BIOL, S-11111 UPPSALA, SWEDEN. MEDIVIR AB, S-14144 HUDDINGE, SWEDEN.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-15 ...
Separase兔多克隆抗体(ab60027)可与人样本反应并经ELISA, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
目录号 规格 价格 说明书下载 PE001-01A 50U 150元 PE001-01B 500U 1000元 重组肠激酶说明 肠激酶(Enterokinase, light chain)是一种高度专一性识别Asp-Asp-Asp-Asp-Lys 序列的蛋白酶,在Lys的C端水解多肽。它可以将胰蛋白酶原转变为胰酶,也可以将带有这个识别序列的融合蛋白切开。由于肠激酶具有高度专一性,水解效率高的特点,它被广泛地应用于基因工程产品的开发,其应用之一是作为工具蛋白酶用于重组融合蛋白质的特异性断裂,尤其适用于生物工
USP provides answers to Frequently Asked Questions (FAQs) as a service to stakeholders and others who are seeking information regarding USPs organization, standards, standards-setting process, and other activities. These are provided for informational purposes only, and should not be construed as an official interpretation of USP text, or be relied upon to demonstrate compliance with USP standards or requirements. USP does not endorse any specific brand or product. For questions not answered here, USP provides multiple routes of support by which the public may seek additional information.
购买USP26兔多克隆抗体(ab101650),USP26抗体经WB验证,可与人样本反应。2篇文献引用,1个独立用户反馈。产品出库一年都在质保范围内。中国现货速达。
Yael Furman, talking about the new Step-Hear-system.. Step-Hear is installed near objects that a visually impaired person needs to find. A hand held unit tells you when you are near, and pressing a button causes a message to be spoken on the base unit.. http://step-hear.com/ ...
Meredith, J.E.; Thompson, L.A.; Toyn, J.H.; Marcin, L.; Barten, D.M.; Marcinkeviciene, J.; Kopcho, L.; Kim, Y.; Lin, A.; Guss, V.; Burton, C.; Iben, L.; Polson, C.; Cantone, J.; Ford, M.; Drexler, D.; Fiedler, T.; Lentz, K.A.; Grace, J.E.; Kolb, J.; Corsa, J.; Pierdomenico, M.; Jones, K.; Olson, R.E.; Macor, J.E.; Albright, C.F., 2008: P-glycoprotein efflux and other factors limit brain amyloid beta reduction by beta-site amyloid precursor protein-cleaving enzyme 1 inhibitors in mice
Looking for online definition of 41-kDa ubiquitin-specific protease in the Medical Dictionary? 41-kDa ubiquitin-specific protease explanation free. What is 41-kDa ubiquitin-specific protease? Meaning of 41-kDa ubiquitin-specific protease medical term. What does 41-kDa ubiquitin-specific protease mean?
Looking for online definition of Deubiquitinating enzyme 46 in the Medical Dictionary? Deubiquitinating enzyme 46 explanation free. What is Deubiquitinating enzyme 46? Meaning of Deubiquitinating enzyme 46 medical term. What does Deubiquitinating enzyme 46 mean?
The human deubiquitinating enzyme ubiquitin-specific protease 2 (USP2) regulates multiple cellular pathways, including cell proliferation and apoptosis. As a result of alternative splicing four USP2 isoenzymes are expressed in human cells of which all contain a weak peroxisome targeting signal of type 1 (PTS1) at their C-termini. Here, we systematically analyzed apoptotic effects induced by overexpression and intracellular localization for each isoform. All isoforms exhibit proapoptotic activity and are post-translationally imported into the matrix of peroxisomes in a PEX5-dependent manner. However, a significant fraction of the USP2 pool resides in the cytosol due to a weaker PTS1 and thus low affinity to the PTS receptor PEX5. Blocking of peroxisomal import did not interfere with the proapoptotic activity of USP2, suggesting that the enzyme performs its critical function outside of this compartment. Instead, increase of the efficiency of USP2 import into peroxisomes either by optimization of its