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We aimed to determine the associations of genetic polymorphisms of excision repair cross-complementation group 1 (ERCC1) rs11615, xeroderma pigmentosum group D (XPD/ERCC2) rs13181, X-ray repair cross complementing group 1 (XRCC1) rs25487, XRCC3 rs1799794, and breast cancer susceptibility gene 1 (BRCA1) rs1799966 from the DNA repair pathway and multiple drug resistance 1 (MDR1/ABCB1) rs1045642 with response to chemotherapy and survival of non-small cell lung cancer (NSCLC) in a Chinese population. ...
Purpose To evaluate the prognostic significance of DNA excision repair gene polymorphisms, excision repair cross-complementation group 1 ( ERCC1) and X-ray repair complementing defective repair in...
XPB antibody (excision repair cross-complementation group 3) for IHC-P, WB. Anti-XPB pAb (GTX55844) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Excision repair cross-complementing group 8 ( ERCC8) plays a critical role in DNA repair. Genetic polymorphisms in ERCC8may contribute to the risk of cancer development. We selected tag single...
Little is known about how the activity of DNA repair nucleases is controlled in cells. Insufficient activity would compromise DNA repair, whereas excessive activity might result in pathological genome cleavage events. In this study, we identify USP45 as a regulator of ERCC1 function in cells. Mass spectrometry analysis identified ERCC1-XPF and the SLX4 scaffold as potentials targets of USP45 together with a number of other proteins that co‐precipitated with USP45. These included MYH9, MYH10, MYL12B, ZFR and RBMX that have previously been reported to interact with USP45 (Sowa et al, 2009). There are two pools of ERCC1-XPF in cells-one is bound to the SLX4 scaffold protein and the other is not (Munoz et al, 2009; Stoepker et al, 2011b). Gel filtration experiments showed that USP45 was associated with both pools of XPF-ERCC1. This observation, together with the findings that USP45 co‐precipitates with XPF-ERCC1 in Slx4 null cells and that ERCC1 interacts with USP45 in a yeast two‐hybrid ...
Little is known about how the activity of DNA repair nucleases is controlled in cells. Insufficient activity would compromise DNA repair, whereas excessive activity might result in pathological genome cleavage events. In this study, we identify USP45 as a regulator of ERCC1 function in cells. Mass spectrometry analysis identified ERCC1-XPF and the SLX4 scaffold as potentials targets of USP45 together with a number of other proteins that co‐precipitated with USP45. These included MYH9, MYH10, MYL12B, ZFR and RBMX that have previously been reported to interact with USP45 (Sowa et al, 2009). There are two pools of ERCC1-XPF in cells-one is bound to the SLX4 scaffold protein and the other is not (Munoz et al, 2009; Stoepker et al, 2011b). Gel filtration experiments showed that USP45 was associated with both pools of XPF-ERCC1. This observation, together with the findings that USP45 co‐precipitates with XPF-ERCC1 in Slx4 null cells and that ERCC1 interacts with USP45 in a yeast two‐hybrid ...
Purpose: Chemoradiation therapy (CRT) is now widely recognized as bladder-preserving therapy for muscle-invasive bladder cancer (MIBC). However, some patients who fail CRT may miss the chance to be cured by cystectomy. Therefore, it is important to select patients with MIBC who are expected to have a good response to CRT. Several reports indicate that the excision repair cross-complementing group 1 (ERCC1) gene is associated with resistance to cisplatin and radiation therapy. In this study, we examined the correlation between ERCC1 and CRT in vitro and in vivo in bladder cancer. Experimental Design: Bladder cancer cell lines T24, 5637, Cl8-2 (multi-drug-resistant subline of T24), and CDDP10-3 (cisplatin-resistant subline of T24) were used for in vitro assays to measure ERCC1 expression level and growth inhibition with cisplatin or irradiation (IR). We then examined by immunohistochemistry whether ERCC1 nuclear staining correlates with the efficacy of CRT using cisplatin in 22 patients with MIBC. ...
A link between DNA damage and the process of aging has been firmly established (1, 2). The brain in particular is a vulnerable organ that is plagued by various neurodegenerative disorders that have been related to aging, i.e. Alzheimer and Parkinson disease. The study of the early onset of age-related neurodegenerative diseases is challenging, because there are not many confident early molecular determinants that predict their development. Therefore, progeroid syndromes (showing premature aging) are often used as a model for segmental aging as they show consistent and predictive elements of the aging phenotype (e.g. cessation of growth and development, hearing loss, and severe and progressive neuron dysfunction) (1, 3). These accelerated aging syndromes have in common that they carry defects in one or multiple proteins involved in DNA damage repair mechanisms.. A well established progeroid mouse model is the excision repair cross-complementing group 1 (Ercc1)1 gene knock-out (4, 5). The ...
casSAR Dugability of Q8BJW7 | Eme1 | Crossover junction endonuclease EME1 - Also known as EME1_MOUSE, Eme1. Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5-end at the branch nick. Typical substrates include 3-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. May self-associate (By similarity). Interacts with MUS81.
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Abnova Human ERCC3 Full-length ORF (NP_000113.1, 1 a.a. - 782 a.a.) Recombinant Protein with GST-tag at N-terminal 25µg Life Sciences:Protein Biology:Proteins:Proteins
References for Abcams Recombinant Human ERCC8 protein (ab114805). Please let us know if you have used this product in your publication
This gene encodes a protein that functions as an assembly component of multiple structure-specific endonucleases. These endonuclease complexes are required for repair of specific types of DNA lesions and critical for cellular responses to replication fork failure. Mutations in this gene were found in patients with Fanconi anemia. [provided by RefSeq, Sep 2016] ...
1BIX: The crystal structure of the human DNA repair endonuclease HAP1 suggests the recognition of extra-helical deoxyribose at DNA abasic sites.
ERCC5 - ERCC5 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 5 (ERCC5) available for purchase from OriGene - Your Gene Company.
Lung cancer is the leading cause of cancer deaths in Taiwan. The carcinogen in the environment is a key role in the development of lung cancer, and one of its main resource is tobacco. Activated carcinogens in the organism lead to mutations of crucial oncogenes resulting in tumor development. Genes such as Cytochrome P-450 family, GST (glutathione S-transferase) family, UGT (UDP-Glucuronosyltransferase) family, ERCC-1(excision repair cross-complementing rodent repair deficiency),ERCC-4 and ERCC-5,are encoding antioxidant enzymes or involving in the DNA repair process and the production of some transcription factors. In recent years, many studies have shown the correlation between these genes and the susceptibility of lung cancer. Each gene has a different role in the tumor development pathway. CYP, UGT, GST, NAT2 (N-acetyltransferase 2) and NQO1(NAD(P)H:quinono oxidoreductase 1) involve in the production of antioxidant enzymes. The antioxidant enzymes can detoxificate hydrogen peroxide or ...
Homo sapiens excision repair cross-complementing rodent repair deficiency, complementation group 8 (ERCC8), transcript variant 2, mRNA. (H00001161-R02) - Products - Abnova
Shop tRNA(fMet)-specific endonuclease ELISA Kit, Recombinant Protein and tRNA(fMet)-specific endonuclease Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
ERCC1 antibody [1C3] (excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)) for FACS, WB. Anti-ERCC1 mAb (GTX84554) is tested in Human samples. 100% Ab-Assurance.
X-Ray Repair Cross Complementing 1 (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 interacts with human…
Complete information for XRCC5 gene (Protein Coding), X-Ray Repair Cross Complementing 5, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ERCC8 gene (Protein Coding), ERCC Excision Repair 8, CSA Ubiquitin Ligase Complex Subunit, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
EME2 forms a heterodimer with MUS81 (MIM 606591) that functions as an XPF (MIM 278760)-type flap/fork endonuclease in DNA repair (Ciccia et al., 2007 [PubMed 17289582]).[supplied by OMIM, Mar 2008 ...
ERCC8兔多克隆抗体(ab107290)可与小鼠, 人样本反应并经WB实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
1. Olaussen KA, Dunant A, Fouret P, Brambilla E, Andre F, Haddad V. et al. DNA repair by ERCC1 in non-small-cell lung cancer and cisplatin-based adjuvant chemotherapy. The New England journal of medicine. 2006;355:983-91 2. Hubner RA, Riley RD, Billingham LJ, Popat S. Excision repair cross-complementation group 1 (ERCC1) status and lung cancer outcomes: a meta-analysis of published studies and recommendations. PLoS One. 2011;6:e25164 3. Allingham-Hawkins D, Lea A, Levine S. ERCC1 Expression Analysis to Guide Therapy in Non-Small Cell Lung Cancer. PLoS Curr. 2010;2:RRN1202 4. Azuma K, Komohara Y, Sasada T, Terazaki Y, Ikeda J, Hoshino T. et al. Excision repair cross-complementation group 1 predicts progression-free and overall survival in non-small cell lung cancer patients treated with platinum-based chemotherapy. Cancer Sci. 2007;98:1336-43 5. Malottki K, Popat S, Deeks JJ, Riley RD, Nicholson AG, Billingham L. Problems of variable biomarker evaluation in stratified medicine research-A case ...
Mung bean nuclease is a nuclease derived from sprouts of the mung bean Vigna radiata that removes nucleotides in a step-wise manner from single-stranded DNA molecules (ssDNA) and is used in biotechnological applications to remove such ssDNA from a mixture also containing double-stranded DNA (dsDNA). This enzyme is useful for transcript mapping, removal of single-stranded regions in DNA hybrids or single-stranded overhangs produced by restriction enzymes, etc. The enzyme degrades single-stranded DNA or RNA to nucleoside 5-monophosphates, but does not digest double-stranded DNA, double-stranded RNA, or DNA / RNA hybrids. Mung Bean Nuclease catalyzes the specific degradation of single-stranded DNA or RNA, and produces mono and oligonucleotides carrying a 5′-P terminus. Mung bean nuclease has a theoretical molecular weight of 39 kDa. Mung bean nuclease has a stringent single-stranded specificity for DNA or RNA and produces 5-phosphoryl oligo- and mononucleotides. Mung bean nuclease requires ...
Currently, quantitative real-time PCR (Q-PCR) of archival formalin-fixed, paraffin embedded (FFPE) tissue is a critical tool for research and is not well established in routine diagnostics. Therefore, continuous improvement in mathematics and statistics associated with interpreting final accurate and reproducible results are fundamental. This project describes and discusses specificity and sensitivity with respect to intra- and inter-assay variances by use of a commercial Human Reference RNA and individual RNA derived from colorectal cancer patients (n = 25). All patients were treated with 5-fluoruracil (5-FU) and a concomitant pelvic radiotherapy (50.4 Gy). Quality assessment of target tissue samples was evaluated by clinicopathological findings and optical density (OD) measurements. We analyzed the steady state messenger RNA (mRNA) expression level of a small panel of cancer relevant genes, excision repair cross-complementing group 1 (ERCC1), ribonucleoside-diphosphate reductase subunit M1 (RRM1),
Structure-specific endonucleases (SSEs) have key roles in DNA replication, recombination and repair, and emerging roles in transcription. These enzymes have specificity for DNA secondary structure rather than for sequence, and therefore their activity must be precisely controlled to ensure genome stability. In this Review, we discuss how SSEs are controlled as part of genome maintenance pathways in eukaryotes, with an emphasis on the elaborate mechanisms that regulate the members of the major SSE families - including the xeroderma pigmentosum group F-complementing protein (XPF) and MMS and UV-sensitive protein 81 (MUS81)-dependent nucleases, and the flap endonuclease 1 (FEN1), XPG and XPG-like endonuclease 1 (GEN1) enzymes - during processes such as DNA adduct repair, Holliday junction processing and replication stress ...
Label-free biosensors (LFBs) have demonstrated a great potential in cost-effective applications, and most of the DNA-based LFBs are based on the principle of binding-induced structural transformation. This review is a collection of the latest reported studies, which have employed structure-selective nucleic Recent Review Articles
ERCC2 - ERCC2 Mutant (L485P), Myc-DDK-tagged ORF clone of Homo sapiens excision repair cross-complementing rodent repair deficiency, complementation group 2 (ERCC2), transcript variant 1 as transfection-ready DNA available for purchase from OriGene - Your Gene Company.
The endo-exonuclease is an enzyme that possesses 5′-3′ exonucleolytic activity with dsDNA and endonucleolytic activity with ssDNA (17, 20, 34, 35). It acts early in the repair of DNA DSB (23). The expression of the endo-exonuclease in cancer cells is much higher than in normal primary cells (Fig. 1 and Table 1) and may be related to the rapid growth of the cancer cell. The expression of the endo-exonuclease is high during the fast-growing phase of the cell cycle and is expressed at very low levels at the stationary phase of the cell cycle (17, 18, 25, 36). In addition, the biological activity of the endo-exonuclease in transfected mammalian cells is shown by an increase in nuclease activity and increased resistance to DNA-damaging agents as well as elevated levels of recombination capacity (22, 37). These unique features of the endo-exonuclease suggest that it might be a target for drug development for the homologous recombination pathway of the DNA DSB repair and cancer. In a search for an ...
Colorectal cancer (CRC) is one of the leading causes of cancer mortality and 5-Fluorouracil (5-FU) is the most common chemotherapy agent of CRC. A high level of X-ray repair cross complementing group 1 (XRCC1) in cancer cells has been associated with the drug resistance occurrence. Moreover, the activation of adenosine monophosphate (AMP)-activated protein kinase (AMPK) has been indicated to regulate the cancer cell survival. Thus, this study was aimed to examine whether XRCC1 plays a role in the 5-FU/AMPK agonist (AICAR)-induced cytotoxic effect on CRC and the underlying mechanisms. Human HCT-116 colorectal cells were used in this study. It was shown that 5-FU increases the XRCC1 expression in HCT-116 cells and then affects the cell survival through CXCR4/Akt signaling. Moreover, 5-FU combined with AICAR further result in more survival inhibition in HCT-116 cells, accompanied with reduced CXCR4/Akt signaling activity and XRCC1 expression. These results elucidate the role and mechanism of XRCC1 in the
Similar to DNA repair endonuclease UVH1 (EC 3.1.-.-) (Ultraviolet hypersensitive 1) (AtRAD1) (DNA excision repair protein XP-F homolog ...
The SCOP classification for the Homing endonucleases superfamily including the families contained in it. Additional information provided includes InterPro annotation (if available), Functional annotation, and SUPERFAMILY links to genome assignments, alignments, domain combinations, taxonomic visualisation and hidden Markov model information.
The SCOP classification for the Homing endonucleases superfamily including the families contained in it. Additional information provided includes InterPro annotation (if available), Functional annotation, and SUPERFAMILY links to genome assignments, alignments, domain combinations, taxonomic visualisation and hidden Markov model information.
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ERCC4, 0.1 ml. The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5 incision made during nucleotide excision repair.
Authors: Tripsianes, Konstantinos; Folkers, Gert; Zheng, Chao; Das, Devashish; Grinstead, Jeffrey; Kaptein, Robert; Boelens, Rolf. Citation: Tripsianes, Konstantinos; Folkers, Gert; Zheng, Chao; Das, Devashish; Grinstead, Jeffrey; Kaptein, Robert; Boelens, Rolf. "Analysis of the XPA and ssDNA-binding surfaces on the central domain of human ERCC1 reveals evidence for subfunctionalization" Nucleic Acids Res. 35, 5789-5798 (2007).. Assembly members: ...
The DST strategy brings together both existing and new methodologies, such as the Ub fusion technique (22, 23); the split-Ub assay (24); ZF DNA-recognizing proteins (43-45); restriction nucleases that are derived either from engineered ZF proteins (37-41) or from homing endonucleases (35, 36) and that are configured (in DST designs) as split nucleases; DNA sequence-enabled assembly of a split protein (31); a new arrangement of split Ub-type domains in a polypeptide chain that enables a double proteolytic cleavage once two chains associate in vivo; and a new feedback mechanism that receives input from a circuit operating as a Boolean OR gate and involves the activation of split nucleases, which destroy the DST vector in normal (nontarget) cells (Figs. 1-3). A certainty that an HD in a cancer cell will be present in that cell and its progeny for as long as those cells endure may lead to a changed perspective on the nature of curative therapies. In what follows, I shall assume, for the sake of ...
DB-ID: Database ID of variant, grouping multiple observations of the same variant together, starting with the HGNC gene symbol, followed by an underscore (_) and a six digit number (e.g. DMD_012345). _000000 is used for variants where DNA was not analysed (change predicted from RNA analysis), variants seen in animal models or variants not seen in humans but functionally tested in vitro ...
GO Process. tRNA splicing, via endonucleolytic cleavage and ligation onclick="removeFacet(GO Process/tRNA splicing, via endonucleolytic cleavage and ligation)"> GO Process tRNA splicing, via endonucleolytic cleavage and ligation ...
1EVX: Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site.
Our observations suggest that variances happen in the processing and the creation of specific fragments that could give an crucial, under-examined system for
He discusses ligation which is related to the fact that there are particular enzymes (ligases) which assist the ends of the DNA strands to join. The process is called ligation. He mentions the Ku factors and DNA-PKcs, which are shown in the graphic in my last post. A heterodimer is a macromolecular complex composed of two different macromolecules. Endonucleases are enzymes which cleave certain chemical bonds. Try to not get bogged down in the jargon ...
The single washing and drying step in the NucleoSpin Plasmid QuickPure miniprep procedure effectively removes endonucleases from the final plasmid miniprep.
DNA-skadande ämnen är vanligt i cancerbehandling, då snabbt växande celler, såsom cancerceller är betydligt känsligare än normala celler för DNA skador. En grupp av ämnen som vanligen används i cancerbehandling är korsbindare av DNA. Dessa ämnen kommer reagera två gånger med DNA och skapa två bindningar mitt emot varandra. DNA strängen, som består av två delar, måste kunna separeras och kopieras (replikation) på ett tillförlitligt sätt för att cellerna ska kunna dela sig och bli flera. DNA strängen måste också kunna dela sig och bli avläst rätt för att nya proteiner ska kunna bildas (transkription). När korsbindarna har bundit till DNA strängarna, hindrar detta deras separation och därigenom förhindras även avläsningen och kopieringen. För att göra undersökningarna av DNA korsbindande ämnen ännu lite svårare, så ger korsbindare flera olika typer av skador. Dels kan det bli flera olika typer av korsbindningar, både mellan två DNA-strängar (ICL) vilket ...
In my hands, Mung Bean S1 Nuclease is easier to use than Nuclease S1. Mung bean is a single-strand-specific nuclease purified from germinated Mung bean sprouts. Degrades DNA and RNA to 5-phosphoryl mononucleotides. (S. C. Sung and M. Laskowski Sr. 1962 J. Biol. Chem. 237: 506.) Single polypeptide chain MW 39,000. Mung bean nuclease is the enzyme of choice over S1 Nuclease for applications where its low activity on dsDNA is desirable. Ratio of activity on ssDNA versus dsDNA is greater than 1500:1 (measured by hyperchromicity by USB ...
161909DNAOphiostoma novo-ulmiWild type I-OnuI 1atggcataca tgtcgcgcag agagtccatc aacccatgga ttctgactgg tttcgctgat 60gccgaaggat ccttcttgct gagaatccga aacaataaca agagctccgt gggttactct 120accgagttgg gctttcaaat cactctgcac aacaaggaca aatcgattct ggagaatatc 180cagtcgactt ggaaggtcgg cgtgattgct aactcaggcg acaatgccgt cagtctgaaa 240gttacgcgtt tcgaagattt gaaagtgatt atcgaccact tcgagaaata tccgctgatt 300acccagaaat tgggcgatta caagttgttt aaacaggcat tcagcgtcat ggagaacaaa 360gaacatctta aggagaatgg gattaaggag ctcgtacgaa tcaaagctaa gatgaattgg 420ggtctcactg acgaattgaa aaaagcattt ccagagaaca ttagcaaaga gcgccccctt 480atcaataaga acattccgaa tttcaaatgg ctggctggat tcacatctgg tgaaggctgc 540ttctttgtga acttgatcaa gtccaaatct aagctgggtg tacaggttca attggtcttc 600agcattactc agcacatcag agacaagaac ctgatgaatt cattgataac atacctaggc 660tgtggttaca tcaaagagaa gaacaagtcc gagttcagtt ggctcgactt tgtggttacc 720aaattcagcg atatcaacga caagatcatt ccggtattcc aggaaaatac tctgattggc 780gtcaaactcg aggactttga agattggtgc aaggttgcca aattgatcga agagaagaaa ...
Aim: Trabectedin sensitivity is increased in cells with functional nucleotide excision DNA repair, whereas efficient homologous recombination repair leads to resistance. On this basis, a retrospective study of mRNA expression of BRCA1 (breast cancer susceptibility 1 gene), XPG (Xeroderma pigmentosum group G gene) and ERCC1 (excision-repair cross complementing group 1 gene) in tumour samples from sarcoma patients treated with trabectedin was conducted, to correlate DNA repair profiles with patient outcome. Materials and methods: Quantification of expression in paraffin embedded tumour samples from 245 patients with advanced sarcomas was performed by qRT-PCR (quantitative real-time polymerase chain reaction). Median values were used as cut-off to define low/high mRNA expression. Results: Low BRCA1 mRNA expression in tumour samples correlated with statistically significant better response to trabectedin. In contrast to other DNA interacting agents, high expression of XPG was significantly ...