TY - JOUR. T1 - Non-cell autonomous cues for enhanced functionality of human embryonic stem cell-derived cardiomyocytes via maturation of sarcolemmal and mitochondrial K ATP channels. AU - Keung, Wendy. AU - Ren, Lihuan. AU - Sen Li, Li. AU - Wong, Andy On Tik. AU - Chopra, Anant. AU - Kong, Chi Wing. AU - Tomaselli, Gordon F.. AU - Chen, Christopher S.. AU - Li, Ronald A.. PY - 2016/9/28. Y1 - 2016/9/28. N2 - Human embryonic stem cells (hESCs) is a potential unlimited ex vivo source of ventricular (V) cardiomyocytes (CMs), but hESC-VCMs and their engineered tissues display immature traits. In adult VCMs, sarcolemmal (sarc) and mitochondrial (mito) ATP-sensitive potassium (K ATP) channels play crucial roles in excitability and cardioprotection. In this study, we aim to investigate the biological roles and use of sarcK ATP and mitoK ATP in hESC-VCM. We showed that SarcI K, ATP in single hESC-VCMs was dormant under baseline conditions, but became markedly activated by cyanide (CN) or the known ...
TY - JOUR. T1 - Transplantation of expanded bone marrow-derived very small embryonic-like stem cells (VSEL-SCs) improves left ventricular function and remodelling after myocardial infarction. AU - Zuba-Surma, Ewa K.. AU - Guo, Yiru. AU - Taher, Hisham. AU - Sanganalmath, Santosh K.. AU - Hunt, Greg. AU - Vincent, Robert J.. AU - Kucia, Magda. AU - Abdel-Latif, Ahmed. AU - Tang, Xian Liang. AU - Ratajczak, Mariusz Z.. AU - Dawn, Buddhadeb. AU - Bolli, Roberto. PY - 2011/6. Y1 - 2011/6. N2 - Adult bone marrow-derived very small embryonic-like stem cells (VSEL-SCs) exhibit a Sca-1+/Lin-/CD45- phenotype and can differentiate into various cell types, including cardiomyocytes and endothelial cells. We have previously reported that transplantation of a small number (1 × 106) of freshly isolated, non-expanded VSEL-SCs into infarcted mouse hearts resulted in improved left ventricular (LV) function and anatomy. Clinical translation, however, will require large numbers of cells. Because the frequency of ...
Movahednia, Mohammad Mehdi Ehdi, Kidwai, Fahad Karim Arim, Zou, Yu, Tong, Huei Jinn, Liu, Xiaochen, Islam, Intekhab, Toh, Wei Seong, Raghunath, Michael, Cao, Tong (2015). Differential effects of the extracellular microenvironment on human embryonic stem cell differentiation into keratinocytes and their subsequent replicative life span. Tissue Engineering - Part A 21 (42223) : 1432-1443. [email protected] Repository. https://doi.org/10.1089/ten.tea. ...
0010] Other researchers have also generated RBCs from ESCs; however, these methods either used non-human/non-primate stem cells or used an embryoid body-dependent method (i.e. no direct differentiation). These methods, however, produced a mixture of erythroid and myeloid cells. See Carotta S, et al., "Directed differentiation and mass cultivation of pure erythroid progenitors from mouse embryonic stem cells," Blood 104:1873-1880 (2004); Chadwick K, et al., "Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells," Blood 102:906-915 (2003); Kaufman D, et al., "Hematopoietic colony-forming cells derived from human embryonic stem cells," Proc. Natl. Acad. Sci. USA 98:10716-10721 (2001); Ng, E, et al., "Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation," Blood 106:1601-1603 (2005); and Zambidis E, et al., "Hematopoietic differentiation of human embryonic stem cells ...
Human embryonic stemcells (hESCs) can serve as a potentially limitless source of cells that may enable regeneration of diseased tissue and organs. Here we investigate the use of human embryonic stemcell-derived cardiomyocytes (hESC-CMs) in promoting recovery from cardiac ischemia reperfusion injury in a mouse model. Using microarrays, we have described the hESC-CM transcriptome within the spectrum of changes that occur between undifferentiated hESCs and fetal heart cells. The hESC-CMs expressed cardiomyocyte genes at levels similar to those found in 20-week fetal heart cells, making this population a good source of potential replacement cells in vivo. Echocardiographic studies showed significant improvement in heart function by 8 weeks after transplantation. Finally, we demonstrate long-term engraftment of hESC-CMs by using molecular imaging to track cellular localization, survival, and proliferation in vivo. Taken together, global gene expression profiling of hESC differentiation enables a ...
QUESTION: I know there have been recent successes with adult stem cell research but why do you say we should eliminate all embryonic stem cell research just because it hasnt been as effective? Im reading articles that say embryonic stem cells hold more potential than adult stem cells so it seems logical to pursue both types of research.. ANSWER: CLR does not consider the lack of success as the reason to reject embryonic stem cell research. In fact, we dont categorically reject all embryonic stem cell research. In our efforts to follow the guidelines in Gods Word we work to defend the lives of all people regardless of age, perceived quality, or status. Therefore we reject any procedure that intentionally destroys human life. Since the current process of harvesting embryonic stem cells involves the killing of embryos we must condemn the process.. Research groups continue to pursue methods of harvesting embryonic stem cells without destroying human lives. If effective harvesting methods are ...
In spite of serious cardiotoxicity side-effects, doxorubicin is frequently used for treatment of several types of cancers. Isolated human adult cardiomyocytes could be the best model for assessing drug-induced cardiotoxicity, while harvesting mature cardiomyocytes is restricted by some limitations such as biopsy size, cell numbers, viability, proliferative capacity and their disability to be passaged as a cell line. In the present study, human embryonic stem cell (hESC)-derived cardiomyocytes applied as a model for evaluation of doxorubicin cardiotoxicity. In this process, cardiogenic differentiated hESCs spheroids were exposed to different concentrations of doxorubicin for 24, 48 and 72 hours. The viability of spheroids as well as their morphology was assessed as important criterion of cardiotoxicity. Findings of the study showed that the viability of spheroids was significantly reduced at doses of 3 and 30 µM (P|0.05). Moreover, cell morphology was changed in the presence of same doses. Overall
A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.
Human embryonic stem cells (hESC) derived from the inner cell mass of pre-implantation human blastocysts have two unique properties-indefinite self-renewal in culture and pluripotency, or the ability to differentiate into tissues from all three embryonic germ layers. As a result, hESC are a promising source of cells for regenerative medicine applications and have enormous potential in modeling human embryonic development. To realize this potential, a deeper understanding of the basic biology of hESC, especially of the genes that regulate self-renewal and differentiation, will be necessary. ❧ The focus of our study is on Oct4, a POU domain transcription factor and critical regulator of pluripotency whose levels are precisely controlled in mouse embryonic stem cells (mESC). In contrast to the single murine Oct4 isoform, which is better understood and more widely studied, three alternatively spliced isoforms exist in humans-OCT4A, OCT4B, and OCT4B1. Studies of human OCT4 are further confounded by ...
... ,R&D Systems adds two new Stem Cell Antibody Panels to its expanding line of stem cell products. The Human Embryonic Stem Cell Marker Antibody Panel (Catalog # SC008) contains the antibodies against: alkaline phosphatase, Nanog, Oct-3/4, SSEA-1 and SSEA-4. The Human Embryonic Stem Cell Marker Antibo,biological,biology supply,biology supplies,biology product
The mitochondrion is emerging as a key organelle in stem cell biology, acting as a regulator of stem cell pluripotency and differentiation. In this study we sought to understand the effect of mitochondrial complex III inhibition during neuronal differentiation of mouse embryonic stem cells. When exposed to antimycin A, a specific complex III inhibitor, embryonic stem cells failed to differentiate into dopaminergic neurons, maintaining high Oct4 levels even when subjected to a specific differentiation protocol. Mitochondrial inhibition affected distinct populations of cells present in culture, inducing cell loss in differentiated cells, but not inducing apoptosis in mouse embryonic stem cells. A reduction in overall proliferation rate was observed, corresponding to a slight arrest in S phase. Moreover, antimycin A treatment induced a consistent increase in HIF-1α protein levels. The present work demonstrates that mitochondrial metabolism is critical for neuronal differentiation and emphasizes that
We have found an element in the cell that controls pluripotency, that is the ability of the human embryonic stem cell to differentiate or become almost any cell in the body," said senior author Kenneth S. Kosik, professor in the Department of Molecular, Cellular & Developmental Biology. Kosik is also co-director and Harriman Chair in Neuroscience Research of UCSBs Neuroscience Research Institute. "The beauty and elegance of stem cells is that they have these dual properties," said Kosik. "On the one hand, they can proliferate -- they can divide and renew. On the other hand, they can also transform themselves into any tissue in the body, any type of cell in the body." The research team includes James Thomson, who provided an important proof to the research effort. Thomson, an adjunct professor at UCSB, is considered the "father of human embryonic stem cell biology." Thomson pioneered work in the isolation and culture of non-human primate and human embryonic stem cells. These cells provide ...
Scientists produce functioning neurons from human embryonic stem cells Neurons will be used to create models of neurological diseases. Thursday, 09 August 2007 Scientists with the Institute of Stem Cell Biology and Medicine at UCLA were able to produce from human embryonic stem cells a highly pure, large quantity of functioning neurons that will allow them to create models of and study diseases such as Alzheimers, Parkinsons, prefrontal dementia and schizophrenia. Researchers previously had been able to produce neurons - the impulse-conducting cells in the brain and spinal cord - from human embryonic stem cells. However, the percentage of neurons in the cell culture was not high and the neurons were difficult to isolate from the other cells. UCLAs Yi Sun, an associate professor of psychiatry and biobehavioral sciences, and Howard Hughes Medical Institute investigator Thomas Südhof at the University of Texas Southwestern Medical Center were able to produce 70 to 80 percent of neurons in cell ...
Scientists have found that the DNA of human embryonic stem cells is chemically modified in a characteristic, predictable pattern. This pattern distinguishes human embryonic stem cells from normal adult cells and cell lines, including cancer cells. The study, which appears online today in Genome Research, should help researchers understand how epigenetic factors contribute to self-renewal and developmental pluripotence, unique characteristics of human embryonic stem cells that may one day allow them to be used for therapeutic cloning.
Peppiatt CM, Collins TJ, Mackenzie L et al. 2-Aminoethoxydiphenyl borate (2-APB) antagonises inositol 1,4,5-trisphosphate-induced calcium release, inhibits calcium pumps and has a use-dependent and slowly reversible action on store-operated calcium entry channels. Cell Calcium 2003; 34:97-108 ...
This protocol describes for the first time, a detailed method to generate vascular smooth muscle cells (SMC) from its three developmental contributers: neuroectoderm, paraxial mesoderm and lateral plate mesoderm progenitor cells, all of which can be derived from human embryonic stem cells. The derived SMCs display contractile ability upon stimulation and have been shown to support vessel formation when transplanted in-vivo. The developmental origin-specific SMC subtypes, enable the study of unique features of the derived SMC subtypes, such as extracellular matrix degradation ...
This protocol describes the generation of a mixed progenitor population from human embryonic stem cells and further isolation of renal cells.. ...
Embryonic stem (ES) cells are pluripotent cells derived from developing mouse blastocysts in vitro that maintain long‐term self renewal and the capacity to give rise to all cell types in the adult body (including some extraembryonic cell types) when subjected to the appropriate conditions
Because video data are complex and are comprised of many images, mining information from video material is difficult to do without the aid of computer software. Video bioinformatics is a powerful quantitative approach for extracting spatio-temporal data from video images using computer software to perform dating mining and analysis. In this article, we introduce a video bioinformatics method for quantifying the growth of human embryonic stem cells (hESC) by analyzing time-lapse videos collected in a Nikon BioStation CT incubator equipped with a camera for video imaging. In our experiments, hESC colonies that were attached to Matrigel were filmed for 48 hours in the BioStation CT. To determine the rate of growth of these colonies, recipes were developed using CL-Quant software which enables users to extract various types of data from video images. To accurately evaluate colony growth, three recipes were created. The first segmented the image into the colony and background, the second enhanced the ...
Sept. 14, 1999: The Wisconsin Alumni Research Foundation establishes WiCell as a clearinghouse to distribute stem cells and foster research.. -- Aug. 9, 2001: President Bush announces his decision to limit federal funding for embryonic stem cell research to cell lines in existence at that point in time.. -- Sept. 4, 2001: A team of Wisconsin scientists led by Dan Kaufman announces it has coaxed stem cells to become blood cells.. -- Nov. 30, 2001: Neural progenitor cells, stem cells that have migrated part way down the developmental pathway to becoming specific types of brain cells, are created and implanted in mice where the cells further develop into functioning neurons. The work was conducted in the laboratory of UW-Madison stem cell scientist Su-Chun Zhang at the Waisman Center.. -- Feb. 10, 2003: Wisconsin scientists James Thomson and Thomas Zwaka report the ability to manipulate genes in human stem cells, a technique critical to studying gene function and creating cells to mimic disease in ...
Effects of hydrodynamic culture on embryonic stem cell differentiation: cardiogenic modulation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
TY - JOUR. T1 - Gene expression patterns of Royan human embryonic stem cells correlate with their propensity and culture systems. AU - Rassouli, Hassan. AU - Khalaj, Mona. AU - Hassani, Seyedeh-Nafiseh. AU - Nemati, Shiva. AU - Salekdeh, Ghasem Hosseini. AU - Baharvand, Hossein. N1 - Copyright the Author(s). Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.. PY - 2019. Y1 - 2019. N2 - Objective: Human embryonic stem cells (hESCs) have the potential to give rise to all types of cells in the human body when appropriately induced to differentiate. Stem cells can differentiate spontaneously into the three-germ layer derivatives by embryoid bodies (EBs) formation. However, the two-dimensional (2D) adherent culture of hESCs under defined conditions is commonly used for directed differentiation toward a specific type of mature cells. In this study, we aimed to determine the ...
Since President Obama repealed research restrictions on human embryonic stem cells (hESCs) in 2009, hESC research has expanded significantly. Before the repeal there were 20 hESC-endorsed lines available for government research funding, now there are 128.. While government funding has been more accessible, investors have still been leery of investing in stem cell companies that use hESCs. There is still a great deal of concern regarding the political environment for this kind of research and many are concerned that if President Obama does not get reelected in 2012 that government funding will again be drastically reduced and that many hESC companies will not be able to survive the cuts. Although the increase in hESC lines available for government funded research has given a boost to the industry, other non-embryonic stem cell work is still funded at a significantly higher rate. According to a recent article in Bloomberg titled "Embryonic Stem-Cell Approvals Rise," the National Institutes of ...
Embryonic stem cells are obtained from early-stage embryos - a group of cells that forms when a womans egg is fertilized with a mans sperm in an in vitro fertilization clinic. Because human embryonic stem cells are extracted from human embryos, several questions and issues have been raised about the ethics of embryonic stem cell research.. The National Institutes of Health created guidelines for human stem cell research in 2009. The guidelines define embryonic stem cells and how they may be used in research, and include recommendations for the donation of embryonic stem cells. Also, the guidelines state embryonic stem cells from embryos created by in vitro fertilization can be used only when the embryo is no longer needed.. The embryos being used in embryonic stem cell research come from eggs that were fertilized at in vitro fertilization clinics but never implanted in a womans uterus. The stem cells are donated with informed consent from donors. The stem cells can live and grow in special ...
Primary stem cell-derived endothelial cells can be used for a variety of purposes (e.g., assays of cell-cell adhesion, migration, vascular tube formation, angiogenesis assays and many other applications) Standard biochemical procedures can be performed using endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, or immunofluorescent staining or flow cytometry, et al.. Primary stem cell-derived endothelial cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics without the express written consent of the company. Cell Biologics Terms and Conditions must be accepted before submitting an order.. Question 9: How much does isolation of stem cell-derived endothelial cells cost? ...
Over 70% of diffuse intrinsic pediatric gliomas, an aggressive brainstem tumor, harbor heterozygous mutations that create a K27M amino acid substitution (methionine replaces lysine 27) in the tail of histone H3.3. The role of the H3.3K27M mutation in tumorigenesis is not fully understood. Here, we use a human embryonic stem cell system to model this tumor. We show that H3.3K27M expression synergizes with p53 loss and PDGFRA activation in neural progenitor cells derived from human embryonic stem cells, resulting in neoplastic transformation. Genome-wide analyses indicate a resetting of the transformed precursors to a developmentally more primitive stem cell state, with evidence of major modifications of histone marks at several master regulator genes. Drug screening assays identified a compound targeting the protein menin as an inhibitor of tumor cell growth in vitro and in mice.
Purpose: A potential application of human embryonic stem cells (hESCs) is the generation of corneal epithelial (CEpi) and endothelial (CEndo) cells to be used for corneal disease treatment. In this study, we designed a new method to induce hESC differentiating to CEpi and CEndo like cells for cornea substitute construction.. Methods: Human embryonic stem cells (hESC) were cultured in a transwell coculture system with differentiated human corneal stromal cells to differentiate into periocular mesenchymal precursor (POMPs). Next, the CEndo-like cells expressed N-cadherin/vimention were derived from POMPs with lens epithelial cell-conditioned medium, and isolated by Fluorescence-activated cell sorting (FACS). To obtain CEpi-like cells, the hESCs were cultured in the limbal stem cells conditioned medium for directional differentiation. The epithelial markers were detected by immunocytochemistry. And then, the induced CEpi and CEndo like cells were cultured on the acellular porcine cornea matrix ...
Epigenetics is the study of changes in gene expression that occur in cells without alterations to DNA sequence. Epigenetic modifications are critical components of eukaryotic gene regulation and chromatin organization. Different epigenetic mechanisms, including the post-translational modifications of DNA-associated histone proteins play a role in the activation or repression of genes. ❧ One of my research goals was to define the epigenetic signature of cultured human embryonic stem cells (hESCs) and to determine how their epigenomes change during lineage commitment. Pluripotent hESCs are capable of self-renewal and have the capacity to differentiate into any lineage of the embryo. However, hESCs grown in culture are heterogeneous in nature, consisting of a mixture of pluripotent to differentiated cells, making investigation of pluripotent hESCs difficult. Therefore precise definition of pluripotent cells present in culture is critical in order to use these cells for future stem cell based ...
Im going to venture in here and throw in my two cents in hopes that you wrote this piece in earnest, not realizing the scientific implications of your accusations and postulations. However, I must say, first and foremost, for the integrity of this piece and any others youve written on human embryonic stem cells, you are not a scientist. As such, it is not only unfair for you to make assumptions, but whats worse is that you are actually distributing your opinion as fact. Describing human embryonic stem cell research as "the killing of young human beings for spare parts" is not only inaccurate, but entirely untrue. It is because of people like yourself that human embryonic stem cells have become synomous with the idea of scientists growing a baby matrix-style and carving it up into little pieces, throwing away the rest. This is NOT what human embryonic stem cell research is in ANY sense.. I agree that there may well be ethical issues in terms of the ways that eggs (oocytes) and embryos are ...
Background and Aims: Human embryonic stem cells (hESCs) are pluripotent cells and thus provide a promising cell source for clinical applications of regenerative medicine. Currently hESCs are cultured on fibroblast feeder cell layers, which provide necessary cell-cell interactions for the attachment and soluble factors enabling the undifferentiated growth of hESCs. However, culturing of feeder cells is expensive and laborious. In addition, xeno-products, used in feeder cell and hESC cultures could transmit animal pathogens to hESCs, and cause rejections when transplanted to patients. Therefore there is a need to develop xeno- and feeder cell-free culturing methods for hESCs. The first aim of this research project was to set up and compare two commercial xeno-products containing feeder cell-free culturing methods for hESCs. The second aim was to optimize a novel, defined, serum- and xeno-free Reges medium, developed in Regea, into feeder cell-free conditions ...
France looked set on Thursday to maintain its curbs on human embryonic stem cell research after the conservative government fought off a parliamentary bid to liberalize the country
After years of animal trials, the first human has been injected with cells from human embryonic stem cells, according to Geron Corporation, the company which is sponsoring the controversial study. This is the first human embryonic stem cell trial in the world, Geron CEO Dr.
Embryonic stem cells are primitive cells derived from a blastocyst, or early stage embryo. These cells have not yet formed into adult stem cells.Embryonic stem cells are referred to as being pluripotent, meaning they have the capacity to become any cell in the human body. Because of this, and their ability to replicate indefinitely, embryonic stem cells can be employed as
ANN ARBOR, Mich. - The University of Michigans first human embryonic stem cell line will be placed on the U.S. National Institutes of Healths registry, making the cells available for federally-funded research. It is the first of the stem cell lines derived at the University of Michigan to be placed on the registry.. The line, known as UM4-6, is a genetically normal line, derived in October 2010 from a cluster of about 30 cells removed from a donated five-day-old embryo roughly the size of the period at the end of this sentence. That embryo was created for reproduction but was no longer needed for that purpose and was therefore about to be discarded.. "This is significant, because acceptance of these cells on the registry demonstrates our attention to details of proper oversight, consenting, and following of NIH guidelines established in 2009," says Gary Smith, Ph.D., who derived the line and also is co-director of the U-M Consortium for Stem Cell Therapies, part of the A. Alfred Taubman ...
Oct4 is one of the master pluripotency genes that controls differentiation of human embryonic stem cells (hESCs). We generated HES2 and HES3 hESC lines stably transduced with lentivirus carrying Oct4 short hairpin RNA (shRNA) that display 80-90% reduction of Oct4 expression. Analysis of pluripotency marker expression shows that these Oct4 shRNA-transduced hESCs display normal wild-type expression levels of the pluripotency marker CD9 but an absence of GCTM2 expression. These hESC-derived adipocyte precursor cells display a characteristic morphology and can be propagated and cryopreserved as a standard stem cell line. Interestingly, Oct4 shRNA-transduced hESCs display a remarkably high lineage-specific spontaneous differentiation toward adipocytes. After two weeks of spontaneous differentiation under feeder-free conditions, 60-70% of cells display a mature adipocyte morphology as well as the expression of multiple adipocyte-specific mRNAs as assessed by RT-PCR. The upregulation of trophoblast, ...
The role of fibroblast growth factor-2 (FGF-2) in maintaining undifferentiated human embryonic stem cells (hESC) was investigated using a targeted phosphoproteomics approach to specifically profile tyrosine phosphorylation events following FGF-2 stimulation. A cumulative total number of 735 unique tyrosine phosphorylation sites on 430 proteins were identified, by far the largest inventory to date for hESC. Early signaling events in FGF-2 stimulated hESC were quantitatively monitored using stable isotope dimethyl labeling, resulting in temporal tyrosine phosphorylation profiles of 316 unique phosphotyrosine peptides originating from 188 proteins. Apart from the rapid activation of all four FGF receptors, trans-activation of several other receptor tyrosine kinases (RTKs) was observed as well as induced tyrosine phosphorylation of downstream proteins such as PI3-K, MAPK and several Src family members. Both PI3-K and MAPK have been linked to hESC maintenance through FGF-2 mediated signaling. The ...
Human embryonic stem cells (hESCs) hold vast promise in science and medicine because of their potential to replicate indefinitely and their capability to differentiate to any cell type found in the adult. Many environmental cues, including soluble factors and intercellular signals, affect hESC differentiation and self-renewal decisions. By integrating a variety of carefully synthesized materials, engineers at the University of Wisconsin have developed a culture system that precisely regulates the size and shape of hESC colonies by confining them to three-dimensional microwells, while providing desired soluble and immobilized chemical factors. By measuring growth and differentiation rates of hESC colonies of different sizes, the UW-MRSEC has identified an optimum self-renewing colony size of 100-200 um diameter; smaller colonies grow slowly, while larger colonies exhibit undesired spontaneous differentiation. Results of this study illustrate the importance of regulating intercellular interactions ...
Despite the enthusiasm for bioengineering of functional renal tissues for transplantation, many obstacles remain before the potential of this technology can be realized in a clinical setting. Viable tissue engineering strategies for the kidney require identification of the necessary cell populations, efficient scaffolds, and the 3D culture conditions to develop and support the unique architecture and physiological function of this vital organ. Our studies have previously demonstrated that decellularized sections of rhesus monkey kidneys of all age groups provide a natural extracellular matrix (ECM) with sufficient structural properties with spatial and organizational influences on human embryonic stem cell (hESC) migration and differentiation. To further explore the use of decellularized natural kidney scaffolds for renal tissue engineering, pluripotent hESC were seeded in whole- or on sections of kidney ECM and cell migration and phenotype compared with the established differentiation assays for hESC.
Diverse types of stem cells represent a potentially attractive source of cardiac cells for the treatment of cardiovascular diseases. However, most of the functional benefits reported for stem cell have been modest and mainly due to paracrine effects rather than differentiation into cardiomyocytes of the applied cells. Therefore, new tools need to be developed in order to improve the efficiency of stem cell differentiation towards specific cardiovascular lineages. Here we show that microRNAs that display early differential expression during ventricular maturation, such as miR-27b, inhibits cardiac differentiation from mouse embryonic stem cells whereas miRNAs that display late differential expression, such as miR-23b, regulates the beating phenotype during in vitro cardiac differentiation from Embryonic Stem Cells (ESCs). This study could have an impact on regenerative medicine since we showed that miR-27b and miR-23b overexpression differentially modify the ESC cell fate towards the cardiac lineage.
For Adult stem cells. Myth: Embryonic stem cell research has the greatest promise. Fact: Up to now, no human being has ever been cured of a disease using embryonic stem cells. Adult stem cells, on the other hand, have already cured thousands. There is the example of the use of bone marrow cells from the hipbone to repair scar tissue on the heart after heart attacks. Research using adult cells is 20-30 years ahead of embryonic stem cells and holds greater promise.. Based on the "The Ten Great Myths in the Debate Over Stem Cell Research" by Tadeusz Pacholczyk, Ph.D. Http://www.ncbcenter.org/10Myths.pdf. Adult stem cells are currently used for the medical treatment of ...
Stem Cells. 2013 Dec;31(12):2759-66. doi: 10.1002/stem.1413. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Govt
Most of the researchers and other people in the field of embryonic stem cell research believed that the existing embryonic stem cells lines were quite inadequate to get some drastic and revolutionary results in bio-medical. It is also not possible under current stem cell policy to diagnose the defects and diseases in embryonic stem cell (Faden and Gearhart). Bush administration has allocated only $25 million share in 2003 for embryonic stem cell research from the $18.3 billion budget of National Institute of Health whereas it allocated $190.7 million for research on adult stem cell which have lot less potential than embryonic stem cell. This approach of Bush administration has retarding effect on embryonic stem cell research and delays the advancement in bio-medical field for an indefinite period ...
Embryonic stem cells are pluripotent, meaning they are able to grow (i.e. differentiate) into all derivatives of the three primary germ layers: ectoderm, endoderm and mesoderm. In other words, they can develop into each of the more than 200 cell types of the adult body as long as they are specified to do so. Embryonic stem cells are distinguished by two distinctive properties: their pluripotency, and their ability to replicate indefinitely. ES cells are pluripotent, that is, they are able to differentiate into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm. These include each of the more than 220 cell types in the adult body. Pluripotency distinguishes embryonic stem cells from adult stem cells found in adults; while embryonic stem cells can generate all cell types in the body, adult stem cells are multipotent and can produce only a limited number of cell types. Additionally, under defined conditions, embryonic stem cells are capable of propagating themselves ...
Human Embryonic Stem Cell Marker Antibody Panel. Contains 25 g each of anti-Alkaline Phosphatase, anti-Nanog, anti-Oct-3/4, anti-SSEA-1, and anti-SSEA-4. from R&D Systems,,biological,biology supply,biology supplies,biology product
b,Objective,/b,: To develop an embryoid body-free directed differentiation protocol for the rapid generation of functional vascular endothelial cells derived from human embryonic stem cells (hESCs) and to assess the system for microRNA regulation and angiogenesis.,p,,/p, ,b,Methods and Results,/b,: The production of defined cell lineages from hESCs is a critical requirement for evaluating their potential in regenerative medicine. We developed a feeder- and serum-free protocol. Directed endothelial differentiation of hESCs revealed rapid loss of pluripotency markers and progressive induction of mRNA and protein expression of vascular markers (including CD31 and vascular endothelial [VE]-cadherin) and angiogenic growth factors (including vascular endothelial growth factor), increased expression of angiogenesis-associated microRNAs (including miR-126 and miR-210), and induction of endothelial cell morphological features. In vitro, differentiated cells produced nitric oxide, migrated across a wound, ...
AIM: Dendritic cell (DC)-based vaccines have a potential utility for use in the treatment of malignancy. Human embryonic stem cells (hESCs) may provide a more cost-effective and reliable source of DCs for immunotherapy purposes, providing on-demand access for patients. METHOD: We developed a protocol to generate DCs from hESCs in vitro in the absence of serum and feeder cells. This protocol uses growth factors bone morphogenetic protein-4, granulocyte macrophage-colony stimulating factor (GM-CSF), stem cell factor and VEGF in serum-free media to generate hESC-derived monocytic cells. These cells are further differentiated to hESC-derived immature DCs with GM-CSF and IL-4, and matured to hESC-derived mature DCs with a maturation cocktail consisting of GM-CSF, TNF-alpha, IL-1beta, IFN-gamma and PGE2. RESULTS: This study demonstrates the applicability of our defined differentiation process in generating functional hESC-derived DCs from multiple hESC lines. We show that hESC-derived immature DCs phagocytose
Embryonic stem cells (ES cells) represent a population of self renewing pluripotent cells, capable of differentiating into derivatives of the 3 embryonic germ layers and are therefore a promising source material for generation of replacement pancreatic beta cells for transplantation in type 1 diabetes. ES cells can be induced to undergo unregulated differentiation when cultured as floating clusters (embryoid bodies). Successful generation of pancreatic endocrine cells from ES cells has thus far relied on embryoid body formation as an initial step. However, selection and expansion of sufficient quantities of insulin -secreting cells has remained a problem. Data presented here show a novel route for ES cell differentiation avoiding embryoid body formation and resulting in a cell population that expresses markers of pancreatic endocrine cell differentiation. The CCE mouse ES cell line was cultured in standard ES cell medium. Cultures were grown to high confluence to create large differentiated ...
Clinical use of mesenchymal stem cells (MSCs) requires a uniform cell population, and their harvesting is invasive and produces a limited number of cells. Human embryonic stem cell-derived MSCs (hESC-MSCs) can differentiate into three germ layers and possess immunosuppressive effects in vitro. Anticancer treatment is a well-known risk factor for premature ovarian failure (POF). In this study, we investigated the effect of hESC-MSC on recovery of ovarian function in cisplatin-induced POF in mice. Female mice received intraperitoneal cisplatin for 10 days. On day 12, CHA15-derived hESC-MSCs were transplanted into the mice by tail vein injection. An injection of PBS served as the negative control. Ovaries were removed 28 days after transplantation for assessment of ovarian histology, immunostaining, and fertility testing by superovulation and in vitro fertilization. hESC-MSC transplantation into mice with cisplatin-induced damage restored body weight and ovary size. Mean primary and primordial follicle
Adult stem cells offer the same pluripotency as embryonic stem cells, but without the danger of forming teratomas (tumors), which remains a serious risk from embryonic stem cells. It is neither necessary nor desirable to use embryonic stem cells in the treatment of spinal cord injuries or other disorders, since a growing number of studies are showing increasing success with adult stem cells. In fact, the only stem cell studies that have ever shown success in the treatment of any human disease have involved adult stem cells, since no study has ever been conducted in which a disease was successfully treated with human embryonic stem cells, although this fact is not generally reported by the media. Ever since researchers first isolated human embryonic stem cells in 1998, there has never been a successful treatment for any human disease in a human being by embryonic stem cells. Embryonic stem cells have in fact proven to be very problematic, whereas bone marrow and cord blood stem cells, by ...
In short, the authors of this study that is just published in Lancet differentiated human embryonic stem cells (hESC) into retinal pigment epithelium (RPE), a key eye tissue that is often affected in retinal degeneration. Then, they transplanted these RPE cells into two patients suffering from Stargardts disease and age-related macular degeneration. The purpose of the trial was to find out whether this procedure is safe. Interestingly, there was a pleasant surprise that both patients seem to have some improvements in eye sight. While there is still a lot to do before stem cells therapy will become widely applicable as a general treatment, this finding is an encouraging first sign.. Reference. Embryonic stem cell trials for macular degeneration: a preliminary report [Lancet][pdf]. ...