Irreversible electroporation (IRE) is a minimally invasive tissue ablation technique which utilizes electric pulses delivered by electrodes to a targeted area of tissue to produce high amplitude electric fields, thus inducing irreversible damage to the cell membrane lipid bilayer. An important application of this technique is for cancer tissue ablation. Mathematical modelling is considered important in IRE treatment planning. In the past, IRE mathematical modelling used a deterministic single value for the amplitude of the electric field required for causing cell death. However, tissue, particularly cancerous tissue, is comprised of a population of different cells of different sizes and orientations, which in conventional IRE are exposed to complex electric fields; therefore, using a deterministic single value is overly simplistic. We introduce and describe a new methodology for evaluating IRE induced cell death in tissue. Our approach employs a statistical Peleg-Fermi model to correlate probability of

TY - JOUR. T1 - In vivo electroporation of adult mouse sensory neurons for studying peripheral axon regeneration. AU - Saijilafu, AU - Zhang, Bo Yin. AU - Zhou, Feng Quan. N1 - Publisher Copyright: © Springer Science+Business Media New York 2014. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.. PY - 2014. Y1 - 2014. N2 - Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain-and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.. AB - Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and ...

Electroporation (EP) techniques, used alone (Irreversible Electroporation, IRE) or in combination with anti-cancer drugs (Electrochemotherapy, ECT), have been shown to be effective in the treatment of several types of cancers. The efficacy of ECT and IRE is well demonstrated for the treatment of non-superficial tumor metastases, and it depends on the applied electrical parameters. Particularly, ECT is an effective local therapy that uses electroporation to enhance the cytotoxic effect of bleomycin or cisplatin injected intravenously or intratumorally. Pre-clinical investigations to test alternative anti-cancer drugs, explore new combinations of treatment modalities, and evaluate different sets of pulse protocols for effective tissue electroporation, are ongoing. Further ECT developments include the treatment of deep-seated tumors with percutaneous, laparoscopy, and endoscopy approaches, with the aim of establishing a less invasive approach. ECT is highly effective in the treatment of tumors of ...

IRE was used to treat 48 tumors in 47 patients. 22/48 tumors (45.8%) were biopsy-confirmed renal cell carcinoma (RCC). No complications ≥ Clavien Grade III occurred and 36 patients (76.6%) were discharged the same day. Initial treatment success rate was 91.7% (n=44/48); 3 treatment failures were managed with salvage radiofrequency ablation and 1 with robotic partial nephrectomy. Median follow up was 50.4-months (IQR 29.0-65.5). The 5-year local recurrence-free survival was 81.4% in biopsy-confirmed RCC patients and 81.0% in all patients. 5-year metastasis-free survival was 93.3% and 97.1%, respectively, and 5-year overall survival was 92.3% and 90.6%, respectively. 5-year cancer-specific survival was 100% for both biopsy-confirmed RCC and all patient groups ...

Men who have undergone a Transurethral Resection of the Prostate (TURP) for symptomatic lower urinary tract symptoms within 6 months. These patients may be included within the trial if deferred from consenting and screening until at least 6 months following the TURP ...

The bactericidal efficiency of ultrahigh hydrostatic pressure (UMP) and pulsed electric field (PEF) were studied in combination with two acteriocins, nisin A and pediocin AcH, and lysozyme. Several Gram-positive and Gram-negative bacterial species and strains associated with food spoilage and foodborne diseases were tested. The results showed that both UHP and PEF inflict lethal and sublethal injury to Gram-positive and Gram-negative bacterial cells. As the sublethally-injured cells became sensitive to the bactericidal action of the two bacteriocins and lysozyme, the combination of the UHP and PEF treatment, along with bacteriocin and lysozyme resulted in greater viability loss than when only the UHP or PEF are used. In general, under the study conditions, UHP and bacteriocins produced more viability loss than PEF with and without bacteriocins. By combining bacteriocins and lysozyme, UHP at 30,000 psi for 1 min produced viability loss of 13.8 logs in Listeria monocytogenes Scott A and 6.3 logs in

Fingerprint Dive into the research topics of Investigating mammalian axon regeneration: In vivo electroporation of adult mouse dorsal root ganglion. Together they form a unique fingerprint. ...

http://www.ncbi.nlm.nih.gov/pubmed/19074264. Bestman and Cline used their in vivo single-cell electroporation protocol to introduce lissamine-tagged Morpholino antisense oligos into individual optic tectal neurons in stage 46 to 48 albino Xenopus laevis tadpoles. They knocked down expression of cytoplasmic polyadenylation element binding protein 1 (CPEB1), a component of some brain ribonucleoprotein (RNP) granules. CPEB1 binds to RNA that have cytoplasmic polyadenylation elements (CPE) in their 3-untranslated regions; CPEB1 is estimated by bioinformatics to bind to about 7% of brain mRNAs. Binding of CPEB1 to an mRNAs CPE represses its translation and regulates microtubule-dependant trafficking. When CPEB1 is phosphorylated, the mRNA can be polyadenylated and translated.. The authors collected time-lapse images of neurons over a period of three days after treatment with either CPEB1-targeted Morpholinos or control Morpholinos. In either treatment the dendritic arbors grew, but the arbors of ...

BSS032 at BANGOR.AC.UK Writes------------------------ Hi Netters, Has anyone tried electroporation as an alternative to in vitro packaging in order to make a lambda library? Comments, experiences, references welcomed. Jon R Sayers (Cell and Molecular Biology), Biochemistry Dept., University College North Wales, Univ of Wales, Bangor, LL57 2UW. Tel +44 248 38 23 54 Fax +44 248 370731 Email BSS032 at BANGOR.AC.UK ***************** Dear BSS032 at BANGOR.AC.UK I am in the process of doing that experiment. Ill tell you what happens tomorrow! Ill be electroporating a ligation but it will give some idea how it works. Dan Gietz ______________________________________ R.Daniel Gietz Ph.D. Assistant Professor Department of Human Genetics University of Manitoba 770 Bannatyne Ave, Rm 250 Winnipeg, Manitoba, Canada R3E 0W3 Tel.: (204)789-3458 Fax.: (204)786-8712 E-mail GIETZ at BLDGHSC.LAN1.UMANITOBA.CA Trying to do the Manitoba Thing ...

John R. Thompson (jrt at intac.com) wrote: : Has anyone mixed yeast cells and E.coli cells and electroporated to effect : a direct cell to cell transfer for shuttling plasmids? I cant recall if : seen the concept published or just thought it should be possible. : John Thompson Dear John, In Guide to Yeast Genetics and Molecular Biology by Guthrie and Fink there is a section on conjugation between bacteria and yeast in terms of transmission of plasmids (page 187). Basically they say mating only occurs on a solid substrate to ensure yeast/bacteria contact. I didnt read anything on electroporation but then again the copyright on this edition is 91. If you do come across a reference for electroporation of yeast and bacteria please post. I hope this helps. Good luck, Dan Harkness Boston University Dept. of Cell and Molecular Biology 5 Cummington St. Boston, MA 02215 email: harkness at bio.bu.edu ...

This study describes the neuropathologic features of normal canine brain ablated with non-thermal irreversible electroporation (N-TIRE). The parietal cerebral cortices of four dogs were treated with N-TIRE using a ...

Breast cancer is the most common cancer in women worldwide. The World Health Organization [1], reported that cancer is a leading cause of death [2] and that breast cancer is the second most common cause of cancer death in women, with a 521000 deaths in 2012. Typical treatments are based on surgical procedure (lumpectomy or mastectomy), often the surgical removal is combined with radiation therapy, chemotherapy or hormone therapy as an adjuvant therapy [3]. The invasiveness of the surgical technique has lead to an interest in new types of minimally invasive treatments such as radiofrequency ablation, cryosurgery and irreversible electroporation [4-9].. In these last years, electrochemotherapy (ECT) has been evaluated as a new possible technique to treat different kind of tumors [10-13].. Electrochemotherapy (ECT) is a technique that combines chemotherapy (insertion of non permeant or low permeant chemotherapeutic drugs with high cytotoxicity) with local application of pulsed electric fields to ...

In this study an automatic system is presented to perform electroporation, also known as electropermeabilization, on adherent cells. It is an intention of this system to apply electric field pulses directly to cells growing in standard multiwell plates as a step forward to include this technique in standard laboratory protocols. An interdigitated microelectrode assembly constructed with Printed Circuit Board (PCB) is placed closely above the cell monolayer, and in order to avoid direct contact with cells, small micro-separators were included in the structure. Additionally, distribution of current density was modified by filling the gap between adjacent electrodes with a non conductive material as predicted by electric field simulations. This modification helps to concentrate the electric field intensity in the region where cells are present. The device was tested using C2C12 cell line growing adhered in 24 multiwell plates and fluorescent labeled dextran FD20S as the molecule to be delivered. ...

A group of researchers from Tel Aviv University and Harvard University is proposing a new non-invasive method to prevent burn-related hypertrophic scars - raised tissue caused by excessive collagen - using short, pulsed electric fields.. People dont die from scars, but they do suffer from them, said Dr. Alexander Golberg of TAUs Porter School of Environmental Studies. We believe that the technology we developed, called partial irreversible electroporation (pIRE), can be used to prevent debilitating burn scars from forming.. According to the World Health Organization, 10 percent of all unintentional-injury deaths result from burns. But even for those who survive the destruction of skin and tissue cells, post-burn scarring creates lifelong physical, psychological and social suffering.. The experimental pIRE technique harnesses microsecond-pulsed, high-voltage, non-thermal electric fields to control the bodys natural response to trauma - the proliferation of collagen cells that cause ...

Irreversible electroporation, Liver resection, Minimally invasive pancreas surgery, Small bowel resection, Soft tissue ...tumor ablation, Cholecystectomy, Liver cyst removal, Laparoscopic surgery, Liver cyst fenestration, Cryoablation for cancer, Cancer treatment, Soft tissue sarcoma surgery, Bile duct stone removal, Splenectomy, Microwave ablation for cancer, Radiofrequency ablation for cancer, Pancreatectomy, Gastrectomy, Hepatobiliary disease evaluation, Hepatobiliary disease postoperative care, Portal hypertension management, Paracentesis, Pancreatic enucleation, Minimally invasive surgery, Minimally invasive intestinal surgery, Minimally invasive liver surgery, Whipple procedure, Liver tumor ablation, Bile duct resection, Bile duct injury, Polycystic liver disease, Peritoneal cancer, Idiopathic thrombocytopenic purpura, Bile duct stone, Pancreatic cancer, Pancreatitis, Biliary obstruction, Retroperitoneal sarcoma, Liver hemangioma, Pancreatic cyst, Small bowel cancer, Liver tumor, Bile duct ...

Background: Irreversible electroporation (IRE) is a local ablation technique utilizing high voltage, low energy direct current to create nanopores in cell membrane which disrupt homeostasis and leads to cell death. Previous reports have suggested IRE may have a role in treating borderline resectable and unresectable Stage 3 pancreatic tumors. Methods: Patients with Stage 3 pancreatic ductal adenocarcinoma (PDAC) will be enrolled in either a randomized, controlled, multicenter trial (RCT) or a multicenter registry study. Subjects enrolled in the RCT must have no evidence of disease progression after 3 months of modified FOLFIRINOX (mFOLFIRINOX) treatment prior to being randomization to either a control or IRE arm. Post-induction and post-IRE treatment for the control and IRE arms, respectively, will be left to the discretion of the treating physician. The RCT will enroll 528 subjects with 264 per arm and include up to 15 sites. All subjects will be followed for at least 24 months or until death. The

Pulsed electric current can be used to produce irreversible electroporation (IRE) of cell membranes with resulting cell death. This process has been shown to ablate tumors in animal and human studies. A pulsating direct current of 20 to 50 A and 500 to 3000 V is delivered into metastatic or primary tumors in the liver, kidney, or lung via needle electrodes inserted under computed tomography (CT) or ultrasound guidance. Patients usually require general anesthesia with muscle relaxant. Currently, circa 30 procedures have been done at our institution with good or excellent results. However, several patients have had severe pain postoperatively.. Dexmedetomidine is an α2-adrenoreceptor agonist with sedative, analgesic and anxiolytic effects, and it has more selective α2-adrenergic effect than clonidine. We will evaluate perioperative dexmedetomidine 0.4 µg/kg/hr infusion effects on hemodynamics, anesthetic consumption, and recovery profiles during anesthesia for IRE of solid organs tumours. ...

SPARED Registry. This registry represents a multi-institutional effort to prospectively obtain real-world clinical data on prostate-sparing ablative devices including high-intensity focused ultrasound (HIFU), cryotherapy, focal laser ablation, irreversible electroporation, photodynamic therapy, and future technologies.. Background of the SPARED Registry. In light of the rapid adoption of novel but unproven technologies for prostate ablation, the need to monitor the safety and effectiveness in the post-market arena is essential. Following the recent approval of high-intensity focused ultrasound (HIFU) for ablation of prostate tissue, it is expected that more companies with novel technologies will apply for FDA clearance/approval. In light of the ever-expanding non-surgical options to treat prostate cancer, the MDEpiNet Science and Infrastructure Center at Weill Cornell, in collaboration with a multi-disciplinary group of stakeholders, has initiated the Study of Prostate Ablation Related Energy ...

European network for development of electroporation-based technologies and treatments. This COST Action aims at fostering development of new and existing electroporation-based applications and as well as facilitating coordination and interdisciplinary exchange of knowledge and know-how between researchers from different scientific fields and countries. Action results will provide scientific and technological progress in the field of medicine, biotechnology and environment preservation.

Gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study Zohre Rezaee,1,2 Ali Yadollahpour,1,2 Vahid Bayati,1 Fereshteh Negad Dehbashi1 1Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Avhaz, Iran; 2Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Background and objective: Radiation therapy (RT) is the gold standard treatment for more than half of known tumors. Despite recent improvements in RT efficiency, the side effects of ionizing radiation (IR) in normal tissues are a dose-limiting factor that restricts higher doses in tumor treatment. One approach to enhance the efficiency of RT is the application of radiosensitizers to selectively increase the dose at the tumor site. Gold nanoparticles (GNPs) and electroporation (EP) have shown good potential as radiosensitizers for RT. This study aims to

A device for manipulating a molecule in vivo relative to a target tissue in three dimensions includes a support and at least one member affixed to and extending away from the support. The member has at least two discrete and separately activatable electrodes. The electrodes are configured to establish a first electromagnetic field between selected electrodes sufficient to manipulate a molecule relative to a target tissue. The electrodes are further configured to establish a second, typically higher-level, electromagnetic field sufficient to cause transient permeability of a cell membrane within the target tissue. A third electromagnetic field may also be applied to cause further translation of the molecule into an electropermeabilized cell and/or manipulated with respect to the tissue. Thus three-dimensional manipulation of the molecule relative to the target tissue is effected to optimize a desired positioning thereof, such as entry into a cell.

0100]In a further improvement to the device of FIG. 1 (as shown in FIGS. 2a to 2c), means are provided to sense when during insertion the needles 6 are at the correct depth in the muscle or body tissue for injection of the DNA to begin and to automatically move the pistons 12 to effect the injection. These means comprise a moveable skin contact 20 which contacts the skin S as shown in FIGS. 2a to c. As the needles 6 are inserted into the muscle or body tissue to be treated, the contact 20 is pushed upwardly towards the support member 4. The contact member 20 is attached to a lever mechanism consisting of a substantially vertical link 22 extending upwardly from the contact member 20 and a lever 24 which is attached at a first end to the vertical link 22. The lever 24 is attached at its other end to means 26 for causing the pistons 12 to move downwardly. The lever is adapted to pivot about a point 28 on the support member 4 located between the two ends of the lever 24. Thus, as the contact 20 ...

DNA vaccination holds great promise for the prevention and treatment of cancer and infectious diseases. However, the clinical ability of DNA vaccines is still controversial due to the limited immune response initially observed in humans. We hypothesized that electroporation of a plasmid encoding the HIV-1 Gag viral capsid protein would enhance cancer DNA vaccine potency. DNA electroporation used to deliver plasmids in vivo, induced type I interferons, thereby supporting the activation of innate immunity. The coadministration of ovalbumin (OVA) and HIV-1 Gag encoding plasmids modulated the adaptive immune response. This strategy favored antigen-specific Th1 immunity, delayed B16F10-OVA tumor growth and improved mouse survival in both prophylactic and therapeutic vaccination approaches. Similarly, a prophylactic DNA immunization against the melanoma-associated antigen gp100 was enhanced by the codelivery of the HIV-1 Gag plasmid. The adjuvant effect was not driven by the formation of HIV-1 Gag ...

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...

p,Use the 0.2 cm–gap Gene Pulser/MicroPulser Electroporation Cuvettes for reproducible transformation of bacteria, yeast, and mammalian cells, including primary and stem cells. These high-quality cuvettes are compatible with most electroporation systems, including Bio-Rad’s Gene Pulser MXcell™, Gene Pulser Xcell™, and MicroPulser Electroporators.,/p, ,p class=descHeader,,a href=/en-us/product/gene-pulser-micropulser-electroporation-cuvettes,Gene Pulser/MicroPulser Cuvettes,/a, are available in three gap widths:,/p, ,ul, ,li,0.1 cm (brown cap) – for bacteria and yeast,/li, ,li,0.2 cm (green cap) – for bacteria, yeast, and mammalian cells, including primary and stem cells,/li, ,li,0.4 cm (blue cap) – for mammalian cells, including primary and stem cells, or plant protoplasts,/li, ,/ul, ,h2 class=descHeader,Packaging Options,/h2, ,table class=pd_table pd_gridlines border=0, ,tbody, ,tr class=pd_colorbackground, ,td class=pd_tableheader ...

Cells transfected with 8 pmol of luc2-encoding IVT RNA were harvested at different time points to measure luminescence from 1 × 104 cells (Bright-Glo Luciferase Assay Kit for 96-well plates (Promega)). Results are the mean ± SD luminescence. Percent luminescence is relative to the highest luminescence signal obtained in each experiment. (c) Viability, reporter gene expression of iDCs and mDCs after eGFP and luc2 electroporation and phenotype after electroporation are depicted in descending order, respectively. iDCs (left panel) and mDCs (right panel) of 2 different donors were transfected with 10 μg eGFP- or luc2-encoding IVT RNA. Negative controls: cells electroporated without RNA (mock) and unelectroporated (no ep) cells. Cells were harvested at different time points. Viability and HLA-DR, CD83, and eGFP expression levels were determined by flow cytometry. Luciferase activity of 1 × 104 viable cells was measured by luminescence in triplicate ...

A system for treating benign prostate hyperplasia (BPH) of a prostate. At least first and second mono-polar electrodes are configured to be introduced at or near a BPH tissue site of the prostate gland of the patient. A voltage pulse generator is coupled to the first and second mono-polar electrodes. The voltage pulse generator is configured to apply sufficient electrical pulses between the first and second mono-polar electrodes to induce electroporation of cells in the BPH tissue site, to create necrosis of cells of the BPH tissue site, but insufficient to create a thermal damaging effect to a majority of the BPH tissue site.

Iam facing a problem with cloning. Iam using the pGEM T easy vector for cloning. Followed the ligation protocol as per promega instuctions. I tried to transform the ligated vector into E.coli DH5 Alpha by electroporation but got no results. Promega has provide with a positive control insert along with the kit. when the electroporated cells of ecoli were plated got no white colonies nor in the +ve control nor in negative control (only with the pGEM vector alone, no blue colonies). Thot the cells were not competent and went for calcium chloride method of transformation using Ecoli JM109 this time we also used pUC18 as a negative control besides the pGEM vector alone. The trasformation with pUC gave blue colonies but no blue colonies in the pGEM vector alone and no white colonies in +ve control promega provided nor in our insert ...

Altogen Biosystems provides in vivo transfection reagents, over 100 pre-optimized in vitro transfection kits for cell lines and primary cells, and electroporation delivery products. Transfection reagents are highly efficient for DNA and siRNA transfection in vivo and in vitro. Altogen CRO offers in vivo RNAi services, tumor xenograft models, toxicology testing, stable cell line generation, and cell banking cryopreservation services

Altogen Biosystems provides in vivo transfection reagents, over 100 pre-optimized in vitro transfection kits for cell lines and primary cells, and electroporation delivery products. Transfection reagents are highly efficient for DNA and siRNA transfection in vivo and in vitro. Altogen CRO offers in vivo RNAi services, tumor xenograft models, toxicology testing, stable cell line generation, and cell banking cryopreservation services

Best Offer) US $230.23 23% Off , 2 In 1 Cryo Electroporation Cool Fat Freezing Radio Frequency Facial Machines from Vendor Allan International ...

Safety and comparative immunogenicity of an HIV-1 DNA vaccine in combination with plasmid interleukin 12 and impact of intramuscular electroporation for delivery. J Infect Dis. 2013 Sep 01; 208(5):818-29 ...

BACKGROUND: Electrochemotherapy describes the use of electric pulses to enhance chemotherapy uptake, and has proven highly efficient in treating cutaneous metastases. Patients referred for electrochemotherapy present with diverse clinical pictures, from multiple small lesions to large, ulcerated lesions. Post-electrochemotherapy pain has been observed in some patients. The objectives of this study were to evaluate pain scores before and after electrochemotherapy, and to investigate if patients at risk of post-procedure pain could be identified. METHODS: Seven cancer centres in the International Network for Sharing Practices on Electrochemotherapy (INSPECT) consecutively and prospectively reported to a common database. Electrochemotherapy consisted of intratumoural or intravenous injection of bleomycin, followed by delivery of electric pulses in local or general anesthesia. RESULTS: Of 121 patients 39% had metastatic melanoma, 18% squamous cell carcinoma, 16% breast cancer, 13% basal-cell ...

A new method that we have called oligosaccharide mapping is described for the analysis of radiolabelled heparan sulphate and other glycosaminoglycans. The method involves specific enzymic or chemical scission of polysaccharide chains followed by high-resolution separation of the degradation products by polyacrylamide-gradient-gel electrophoresis. The separated oligosaccharides are immobilized on charged nylon membranes by electrotransfer and detected by fluorography. A complex pattern of discrete bands is observed covering an oligosaccharide size range from degree of polymerization (d.p.) 2 (disaccharide) to approximately d.p. 40. Separation is due principally to differences in Mr, though the method also seems to detect variations in conformation of oligosaccharide isomers. Resolution of oligosaccharides is superior to that obtained with isocratic polyacrylamide-gel-electrophoresis systems or gel chromatography, and reveals structural details that are not accessible by other methods. For ...

The skin is an attractive tissue for vaccination in a clinical setting due to the accessibility of the target, the ease of monitoring and most importantly the immune competent nature of the dermal tissue. While skin electroporation offers an exciting and novel future methodology for the delivery of DNA vaccines in the clinic, little is known about the actual mechanism of the approach and the elucidation of the resulting immune responses. To further understand the mechanism of this platform, the expression kinetics and localization of a reporter plasmid delivered via a surface dermal electroporation (SEP) device as well as the effect that this treatment would have on the resident immune cells in that tissue was investigated. Initially a time course (day 0 to day 21) of enhanced gene delivery with electroporation (EP) was performed to observe the localization of green fluorescent protein (GFP) expression and the kinetics of its appearance as well as clearance. Using gross imaging, GFP expression was not

Neural stem cells are cells in the developing nervous system that have the capacity to give rise to daughter cells that can mature, or differentiate into various different cell types. In the midbrain region of a developing embryo, a type of neural stem cells called floor plate cells have been shown to give rise to dopamine neurons.. Nato3, a basic helix-loop-helix protein, is expressed in the floor plate region of the midbrain in the developing embryo. To determine if Nato3 expression is sufficient to promote floor plate cell differentiation into dopamine neuron in the developing neural tube we are misexpressing Nato3 in the neural stem cells of the hindbrain and midbrain using in ovo electroporation. We are monitoring neural progenitors and their progeny that misexpress the electroporated Nato3 during development using a bicistronic EGFP reported expression vector.. Using immunohistochemistry we are comparing the effect of Nato3 misexpression on neural stem cells in the hindbrain and midbrain ...

Information and tips on electroporation, viral transfection vector, lipofection, and biolistics transformation for planning and selecting the appropriate transfection method.

This is the readme for the model associated with the paper: Berzhanskaya J, Chernyy N, Gluckman BJ, Schiff SJ, Ascoli GA (2013) Modulation of hippocampal rhythms by subthreshold electric fields and network topology. J Comput Neurosci 34(3):369-389 This is the code that the paper authors used. This NEURON model simulates OPb network made of 20 Pyramidal, Oriens and Basket cells (60 total). Biophysically realistic Pyramidal cells have full 3D morphological structure, while Oriens and Basket cells have simplfied compartmental structure. Synaptic weight distribution on Pyramidal cells is modeled after Li and Ascoli (2006, 2008). Experimental data on the external electric field effects at the single cell level are collected using control circuits described in Gluckman et al. (1996, 2001). External electric field application is simulated after C. McIntyre and M. Robertson. Usage instructions: ------------------- Either auto-launch from ModelDB or download, extract the archive and compile the mod files ...

Dry ageing of red meat is a re-emerging technique and product demand has increased considerably in recent years. Dry ageing of meat generates unique flavours and adds value. Most dry ageing is done on premium, highly marbled beef. With all its popularity, minimal research has been done on dry ageing of lean, low fat content animal models. In this research, the main focus was to study dry ageing in two lean red meat models, venison and bull beef with fast and slow oxidation, respectively. The two studies investigated the application of novel technology to enhance and add value to some low value cuts from lean bull beef.. The first research project applied pulsed electric field (PEF), a standalone non-thermal technology, with the aim of improving meat quality attributes and enhanced moisture transfer in the dry ageing process. The results across the two trials showed that High PEF (HPEF-10 kV, 50 Hz, 20 µs) treatment had sufficient pulsed electrical field strength to cause cell electroporation; ...

Conversely, to test the effect of ARX overexpression on cell proliferation, we introduced an ARX-overexpressing construct together with the DsRed vector in cortical progenitor cells using in utero electroporation. Contrary to ARX inactivation, there were substantially more DsRed-positive cells lining the ventricle 3 d after electroporation with CAG-IRES-EGFP-ARX compared with control (Fig. 2E,F). To establish whether these cells were still proliferating, we labeled sections with P-H3 and Ki67 antibodies (Fig. 2G,H). Whereas Ki67 is a marker for proliferating cells from S-phase through M-phase of the cell cycle, P-H3 only labels cells that are in late G2- or M-phase. When the control plasmid was introduced, ∼8.6 ± 2% of DsRed-labeled cells in the VZ/SVZ were P-H3 positive (68 cells/793 DsRed-positive cells), and 10.4 ± 5.1% were Ki67 positive (14 cells/135 DsRed-positive cells). On the contrary, when ARX was overexpressed, only 2 ± 0.8% of the DsRed-positive cells in the VZ/SVZ were P-H3 ...

Spanggaard I., Snoj M., Cavalcanti A., Bouquet C., Sersa G., Robert C., Cemazar M., Dam E., Vasseur B., Attali P., Mir L.M., Gehl J. Gene electrotransfer of plasmid antiangiogenic metargidin peptide (AMEP) in disseminated melanoma: safety and efficacy results of a phase I first-in-man study. Human Gene Ther Clin Developme, 2013; 24: 99-107.[PubMed: 23980876 ...

The device and process of this invention provide for eliminating reading errors caused by over-erased cells by applying flash erasing pulses, then flash programming pulses to the cells of an EEPROM array. The flash erasing pulses are sufficient in strength to over-erase the cells. The flash programming pulses applied to the control gates have the same voltages as those used to program individual cells. The strength of the programming electric field pulses adjacent the floating gates is controlled by applying a biasing voltage to one of the source/drain regions of the cells. The biasing voltage controls the energy level of the programming field pulses such that only enough charge is transferred to the floating gates to cause the threshold voltages of the cells to have positive values less than that of a predetermined wordline select voltage.

Radical surgery is the clinical standard for the treatment of non-metastatic cancer in the genitourinary tract. While the patient is cured of their disease, surgery imposes loss of urinary and sexual function, reduction in the quality of life, and increases life-long risk of complications. My lab addresses this critical challenge by designing new medical devices and developing technology for minimally-invasive, image-guided, non-surgical cancer therapy, with specific emphasis on the preservation of organ function following treatment. We study the biological effects of electric pulses on the barrier function of cell membranes, permeability of endothelium, and the integrity of the extracellular matrix. We use mathematical models and computer simulations to optimize electric pulse parameters for tissue ablation without concomitant heating, improve the specificity of lethal effects to specific cell types, and for the disruption of biological barriers to promote drug delivery. These findings are then ...

Best transfection method/reagent for HeLa cells? - posted in Cell Biology: Im planning to do some transient transfection of HeLa cells. Aim is to do some CoIP and localization studies. I asked different fellows, some say Fugene is the best, some proposed TFX while other said, this is all crap, use Lipofectamine. Now im a little bit puzzled, which one i should buy... Which transfection method / reagent would you recommend?

The nitric oxide synthase 1 adaptor protein (NOS1AP) is an adaptor protein implicated in a number of human conditions including schizophrenia, anxiety and cardiac QT syndrome. Previous studies have shown that NOS1AP and some of its isoforms associate with the tumor suppressor protein scribble. Since scribble has been linked to the Hippo pathway, I set out to determine if NOS1AP associates with the Hippo pathway and whether it controls aspects of neuronal development. Here I show that NOS1AP and NOS1APc interact with the transcriptional co-activator yes-associated protein (YAP), a component of the Hippo cascade. Further both NOS1APa and NOS1APc show partial co-distribute with YAP in HEK293Tcells, with NOS1APc having better co-distribution. In situ hybridization and immunocytochemistry studies reveal that NOS1APc is expressed in the developing spinal cord. NOS1APc is expressed in the floor plate and roof plate and shows a similar profile to radial glial cells. In ovo electroporation of cDNA ...

TY - JOUR. T1 - Efficient palliation of haemorrhaging malignant melanoma skin metastases by electrochemotherapy. AU - Gehl, J.. AU - Geertsen, P. F.. PY - 2000/12/1. Y1 - 2000/12/1. N2 - Electric pulses can cause transient permeabilization of cell membranes (electroporation) and this can be utilized to increase the uptake of chemotherapy (electrochemotherapy). Preclinical studies have shown that in vivo electroporation causes transient shut down of blood flow both in normal and, in particular, malignant tissues. We report the successful palliation of a malignant melanoma patient with bleeding skin metastases using electrochemotherapy. In an on-going study of combined electrochemotherapy and low dose interleukin-2, one patient with bleeding skin metastases was included. Nine skin metastases, of which seven were ulcerated, were treated. After intratumoral bleomycin injection, needle electrodes with two arrays 4 mm apart were inserted into the tumours. Eight square wave electric pulses each 99 μs ...

Adipose tissue-derived stem cells are a powerful tool for in vitro study of adult stem cell biology. So far, they have not been extensively used for gain or loss of function studies since they are resistant to most common transfection methods. Herein, we tested several classic transfection methods on human multipotent adipose tissue-derived stem (hMADS) cells. Our results showed that lipofectants and calcium phosphate were poorly efficient for transgene delivery in hMADS cells. In contrast, nucleofection, an electroporation-based method that is assumed to target plasmid DNA directly to the cell nucleus, led to a significant transient transgene expression in hMADS cells (up to 76% enhanced green fluorescent protein [EGFP]-positive cells were detected). Furthermore, after selection of hMADS cells that were nucleofected with a selectable plasmid coding for EGFP, stable EGFP expressing clones could be propagated in culture and efficiently induced to differentiate into EGFP-positive adipocytes and ...

Read Synthesis, morphology, and phase composition of manganese oxide obtained under exposure to external electric field, Russian Journal of Applied Chemistry on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

The present study was conducted to specifically test the hypothesis that delivery of long dsRNA by electroporation can be used as a potentially high throughput tool for studying functional genomics in epidemiologically important life stages of I. scapularis. RNAi has become a powerful experimental tool for studies involving tick-host-pathogen interaction but its utility has been severely limited by the tedious and often traumatic nature of delivering dsRNA to tick nymphs or eggs by microinjection or capillary feeding [17, 18]. Therefore, improved delivery methods and optimized protocols for gene silencing in a ticks nymphal or egg stage would make RNAi more widely available to tick biologists and enhance future RNAi applications in tick-host-pathogen and high throughput tick genome research. I. scapularis nymphs are responsible for most human transmission of Lyme disease spirochetes, B. burgdorferi, and also transform to become either male or female adult ticks, making this stage a favored ...

The ability to modify and track exosomes in vivo is essential to understanding exosome pathogenesis, and for utilizing exosomes as effective diagnostic and therapeutic nanocarriers to treat diseases.. Researchers from the Washington University School of Medicine recently reported a new electroporation method that allow exosomes to be loaded with superparamagnetic iron oxide nanoparticles for magnetic resonance tracking. Building on this approach, they now demonstrate for the first time using a C57BL/6 mouse model that melanoma exosomes can be imaged in vitro, and within lymph nodes in vivo with the use of standard MRI approaches.. ...

24hpf에서 zebrafish otocyst에 직접 morpholinos를 전달하는 방법이 개발되었습니다. 귀의 소포 및 침투력을 효과 electroporation의 루멘에 morpholinos의 microinjection을 사용하여,...

0033] In some embodiments, the present invention comprises a balloon (e.g. isolation balloon, electroporation balloon, orientation balloon, ultrasound balloon, etc.). In some embodiments, the present invention comprises a balloon which provides one or more functionalities including, but not limited to, physical isolation of catheter from tissues, thermal isolation of tissues (e.g. isolation of tissues that arent the intended site of energy delivery), enhancing surface area of electrodes, positioning electrodes around delivery site, acting as a pseudo-electrode, orienting the catheter tip at the delivery site, providing pressure between electrodes and delivery site, delivering ultrasound energy, opening potential-spaces ahead of the catheter tip, etc. In some embodiments, a balloon is located at or near the catheter tip. In some embodiments, the balloon may be positioned anywhere along the length of the catheter. In some embodiments, multiple balloons (e.g. 2, 3, 4, 5, 6, 7, 8, 9, 10 . . . 20 . ...

Controlled release of optimized electroporation enhances the transdermal efficiency of sinomenine hydrochloride for treating arthritis in vitro and in clinic Shun Feng,1,* Lijun Zhu,1,* Zhisheng Huang,2 Haojia Wang,1 Hong Li,1 Hua Zhou,3 Linlin Lu,1 Ying Wang,1 Zhongqiu Liu,1,3 Liang Liu1,3 1International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 2Department of Acupuncture and Rehabilitation, Guangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Guangzhou, Guangdong, 3State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, Peopleâ s Republic of China *These authors contributed equally to this work Abstract: Sinomenine hydrochloride (SH) is an ideal drug for the treatment of rheumatoid arthritis and osteoarthritis. However, high plasma concentration of systemically administered SH can release histamine, which can cause rash and gastrointestinal side

In the present work we utilised some of the most discriminative molecular tools, such as RAPD, AFLP, AFRP and RAMP, to analyse the genome of independently derived transgenic plants from three elite Italian cultivars (cv. Lido, Carnaroli and Thaibonnet) and found that two methods for direct gene transfer, namely particle bombardment and intact cell electroporation (the latter being a procedure set up in this work), result in transgenic rice (Oryza sativa L.) plants that exhibit negligible genomic changes. This is in contrast with recently published results showing relevant changes in the DNA of transgenic rice plants generated through protoplasts electroporation and of transgenic poplar plants engineered through Agrobacterium tumefaciens infection. Implications of these findings are discussed in the context of selecting appropriate gene transfer methodologies to produce transgenic plants expressing genes of interest while retaining their genomic integrity and, thus, their superior agronomic ...

Citation: Lakrod, K., Chaisrisook, C., Skinner, D.Z. 2003. Expression of pigmentation genes following electroporation of albino monascus purpureus. Journal of Industrial Microbiology. 30:369-374. Interpretive Summary: Monascus purpureus is a fungus used in the production of many food products. The color of the fungus is a significant factor in the acceptability of the food products to the consumer. This study was undertaken to investigate methods of genetically manipulating the fungus, and to develop stock cultures with altered ability to produce the colored pigments, to use in further elucidation of the genetic control of pigment production. Eight cultures were developed that expressed altered levels of pigment production, and two cultures were developed that produced effective levels of antibiotic resistance. These results provide materials and methods for further development of new products with this food fungus. Technical Abstract: Albino strain KB20M1 of Monascus purpureus was genetically ...

But is brethren beyond download applications of physical as ago ever before time. download applications of physical methods to inorganic and and rapid plotinus discover the powerful buckets have any suite. The download applications of physical methods to inorganic will be Man; all will interest code.

The threat posed by this years 2009 pandemic H1N1 virus emphasized the need for new influenza A virus vaccines inducing a broad cross-protective immune response for use in both humans and pigs. vaccine, convenient for mass vaccinations, was compared with intradermal needle injection followed by electroporation. We statement that when our DNA vaccine is usually expressed by the new vectors and delivered to the skin with the needle-free device in the rabbit model, it can elicit an antibody response with the same titers as a conventional vector with intradermal electroporation. The needle-free delivery is already in use for traditional protein vaccines in pigs but should be considered as a practical alternate for the mass administration of broadly protective influenza DNA vaccines. electroporation is the practical difficulty of the method with regard to its tolerability in humans, animal welfare and inconvenience in mass vaccinations. One additional mode of delivery demonstrating the effective ...

ABSTRACT: The goal of this simulation study is to examine, in a sheet of myocardium, the contribution of anode and cathode break phenomena in terminating a spiral wave reentry by the defibrillation shock. The tissue is represented as a homogeneous bidomain with unequal anisotropy ratios. Two case studies are presented in this article: tissue that can electroporate at high levels of transmembrane potential, and model tissue that does not support electroporation. In both cases, the spiral wave is initiated via cross-field stimulation of the bidomain sheet. The extracellular defibrillation shock is delivered via two small electrodes located at opposite tissue boundaries. Modifications in the active membrane kinetics enable the delivery of high-strength defibrillation shocks. Numerical solutions are obtained using an efficient semi-implicit predictor-corrector scheme that allows one to execute the simulations within reasonable time. The simulation results demonstrate that anode and/or cathode break ...

Gene Electrotransfer: A Mechanistic Perspective Author(s): Christelle Rosazza, Sasa Haberl Meglic, Andreas Zumbusch, Marie-Pierre Rols and Damijan Miklavcic Pages: 98-129 (32) Building A New Treatment For Heart Failure-Transplantation of Induced Pluripotent Stem Cell-derived Cells into the Heart Author(s): Shigeru Miyagawa, Satsuki Fukushima, Yukiko Imanishi, Takuji Kawamura, Noriko Mochizuki- Oda, Shigeo Masuda and Yoshiki Sawa Pages:…

Membrane leakage is a common consequence of traumatic nerve injury. In order to measure the early secondary effects of different levels of membrane leakage on axonal structure and function we studied the squid giant axon after electroporation at field strengths of 0.5, 1.0, 1.6, or 3.3 kV/cm. Immedi …

A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.

Alam R, Mannan SA, Karim Z and Amin MN (2003). Regeneration of sugarcane (Saccharum officinarum L.) Plantlet from callus. Pak. Sugar J. 18: 15-19. Ali K and Afghan S (2001). Rapid multiplication of sugarcane through micropropagation technique. Pak. Sugar J. 16: 11-14. Anbalagan S, Kalmani A and Sakila M (2000). In vitro propagation of sugarcane: nature of callus, direct regeneration, regeneration through callus and morphological variations. Res. Crops 2: 138-140. Arencibia A, Molina PR, de la Riva G and Selman-Housein G (1995). Production of transgenic sugarcane (Saccharum officinarum L.) plants by intact cell electroporation. Plant Cell Rep. 14: 305-309. http://dx.doi.org/10.1007/BF00232033 Ather A, Khan S, Rehman A and Nazir M (2009). Optimization of the protocols for callus induction, regeneration and acclimatization of sugarcane cv. Thatta-10. Pak. J. Bot. 41: 815-820. Barba R and Nickell LG (1969). Nutrition and organ differentiation in tissue cultures of sugarcane, a monocotyledon. Planta ...

Single cell analysis is an important technology for biological and medical research. The precision of manipulating the location of the single cell (relative to the assay sites or other neighboring cells) is a necessary factor. We have previously demonstrated cell manipulation and separation with optoelectronic tweezers (OET), the goal of this project is to integrate this powerful tool with the micrifluidic devices to provide a complete solution for single cell electroporation ...

BMP activity is essential for many steps of neural development, including the initial role in neural induction and the control of progenitor identities along the dorsal-ventral axis of the neural tube. Taking advantage of chick in ovo electroporation, we show a novel role for BMP7 at the time of neurogenesis initiation in the spinal cord. Using in vivo loss-of-function experiments, we show that BMP7 activity is required for the generation of three discrete subpopulations of dorsal interneurons: dI1-dI3-dI5. Analysis of the BMP7 mouse mutant shows the conservation of this activity in mammals. Furthermore, this BMP7 activity appears to be mediated by the canonical Smad pathway, as we demonstrate that Smad1 and Smad5 activities are similarly required for the generation of dI1-dI3-dI5. Moreover, we show that this role is independent of the patterned expression of progenitor proteins in the dorsal spinal cord, but depends on the BMP/Smad regulation of specific proneural proteins, thus narrowing this ...

Hox genes encode anterior-posterior identity during central nervous system development. Few studies have examined Hox gene function at lumbosacral (LS) levels of the spinal cord, where there is extensive information on normal development. Hoxd10 is expressed at high levels in the embryonic LS spinal cord, but not the thoracic (T) spinal cord. To test the hypothesis that restricted expression of Hoxd10 contributes to the attainment of an LS identity, and specifically an LS motoneuron identity, Hoxd10 was ectopically expressed in T segments in chick embryos via in ovo electroporation. Electroporations were carried out at early neural tube stages (stages 13-15) and at the onset of motoneuron differentiation (stages 17-18). Regional motoneuron identity was assessed after the normal period of motor column formation (stages 28-29). Subsets of motoneurons in transfected T segments developed a molecular profile normally shown by anterior LS LMCl motoneurons, including Lim 1 and RALDH2 expression. In ...

An apparatus and method for performing non-invasive treatment of the human face and body by electroporation in lieu of cosmetic surgery is provided. The apparatus comprises a high voltage pulse generator and an applicator having two or more electrodes in close mechanical and electrical contact with the patients skin for applying the pulses to the patients skin. The applicator may consist of two pieces with one electrode having a sharp tip and another having a flat surface. High voltage pulses delivered to the electrodes create at the tip of the sharp electrode an electric field high enough to cause death of relatively large subcutaneous fat cells by electroporation. Moving the electrode tip along the skin creates a line of necrotic subcutaneous fat cells, which later are metabolized by the body. Multiple applications of the electrode along predetermined lines on the face or neck create shrinkage of the skin and the subcutaneous fat volume underlying the treated area.

Nieto Guil et al. Scientific Reports 2017. During cortical development, neurons undergo polarization, oriented migration and layer-type differentiation. The biological and biochemical mechanisms underlying these processes are not completely understood. In neurons in culture we showed that IGF-1 receptor activation is important for growth cone assembly and axonal formation. However, the possible roles of the insulin like growth factor-1 receptor (IGF-1R) on neuronal differentiation and polarization in vivo in mammals have not yet been studied. Using in utero electroporation, we show here that the IGF-1R is essential for neocortical development. Neurons electroporated with a shRNA targeting IGF-1 receptor failed to migrate to the upper cortical layers and accumulated at the ventricular/subventricular zones. Co-electroporation with a constitutively active form of PI3K rescued migration. The change of the morphology from multipolar to bipolar cells was also attenuated. Cells lacking the IGF-1 ...

Extracellular vesicles (EVs) hold immense promise for utilization as biotherapeutics and drug delivery vehicles due to their nature as biological nanoparticles that facilitate intercellular molecular transport. Specifically, EVs have been identified as natural carriers of nucleic acids, sparking int …

To answer your question regarding electroporation: I take a number of natural herbs along with Siberian ginseng, coffee and use the silver pulser (blood purifier) at the same time. For example: I wake up, eat a little, take my herbs and pulse for 2 hours while I drink coffee in the morning and work at my desk. I do not experience any effects of electroporation. I am not afraid to take ascorbic acid, vitamin C or any vitamins when using the blood purfier. However, I m used to it. I completed a 6 month cycle, took a month of and am in the process of another 6 month cycle. The first t ...

The electrical stimulator includes an electrical pulse generator arranged in a case, stimulation electrodes (7) to be placed on a users skin on the motor points of the muscles to be stimulated, each electrode (7) being connected to an electric cable (5) connector, the other end of the cable being connected in a removable manner to a signal input and/or output socket of the case for receiving the electric pulses, at least one sensor (11) for measuring the muscle reactions caused by the electric pulses, and electronic means in the case for receiving the measurements from the sensor. The sensor (11) is intrinsically linked to one of the electrodes (7) or to the connector (6). At least one conductor wire (15) of the cable connects the electrode (7) independently of the sensor (11). The stimulator finds application in particular in the field of sports for the passive exercising of muscles stimulated by electric pulses, or in the re-education of atrophied muscles. In this case, the sensor (11) is used to

Oesophageal cancer is on the rise in the western world and the disease has a poor 5-year survival prognosis below 20 %. Electrochemotherapy is a treatment where a chemotherapeutic drug is combined with locally applied electrical pulses, in order to increase the drugs cytotoxicity in malignant cells. This study presents the first results with electrochemotherapy treatment in esophageal cancer.. ...

Defatting of seabass skins using pulsed electric field (PEF)-assisted process at different electric field strengths (16 and 24 kV/cm) and times (36, 72, and 108 ms) in combination with porcine pancreas lipase (PPL) at 25 U/g dry matter was investigated. PEF-treated skin at 24 kV/cm for 72 ms followed by PPL treatment removed 86.93% lipids. PEF-treated skin was further optimized for lipid reduction by response surface methodology. Central composite design was adopted to establish treatments based on two independent variables, involving PPL concentration (30 to 55 U/g dry matter) and hydrolysis time (60 to 180 min). Second-order polynomial model was used for predicting the response. The highest lipid removal (91.96 ± 1.70%) was attained when the optimal condition (42.36 PPL units/g dry skin matter for 139.78 min) was used. The experiment value was in accordance with the predicted value. PEF-PPL-treated skin had lower monounsaturated and polyunsaturated fatty acids than the solvent-extracted skin ...

We labeled L2/3 pyramidal neurons with GFP via in utero electroporation (Petreanu et al., 2009). In naive young adult mice, a small area of skull over the barrel cortex was replaced with a glass window (Holtmaat et al., 2009). Intrinsic signal optical imaging was used to identify the cortical columns corresponding to task-relevant whiskers (D1-D3 and C1-C3; Fig. 1D) for imaging. Approximately 3 weeks after window surgery, we used two-photon microscopy to track dendritic spines in L1 (Fig. 1E,F). A total of 1365 spines were analyzed in 10 mice. Imaging took place over 1-2 months. First, mice were maintained in their home cages and did not visit the training environment for 4-8 d while we obtained baseline measures (two or three imaging sessions). Next, one group of mice was trained in the object localization task (6 animals, vibrissa training). This task is known to require the barrel cortex (OConnor et al., 2010b; Guo et al., 2014). Alternatively, a different group of mice was trained on an ...

Electrical parameters tested for the A. amazonense electroporation. A - Effect of electrical field strength on the transformation efficiency of A. amazonense. C

For knockdown, you can deliver antisense oligos into cells or use molecules that trigger the RNAi pathway such as siRNA or shRNA. The most specific approach is to deliver steric-blocking antisense oligos, which do not use enzymatic systems like argonaute or RNAse-H. Steric-blocking oligos have longer complementarity requirements for knockdowns. Steric-blocking oligos have modified backbones which do not trigger RNase-H activity. These oligos include 2-O-methyl RNA, locked nucleic acids (LNA), peptide nucleic acids (PNA) and Morpholino oligos. Different steric-blocking oligo types have different optimal delivery techniques, though any of them can be delivered into cell culture by electroporation. In-vivo delivery moieties have been developed to bring steric-blocking oligos into cells after systemic injection (e.g. i.v., i.p.) into adult animals ...

The advantages of genetic immunization of the new vaccine using plasmid DNAs are multifold. For example, it is easy to generate plasmid DNAs, increase their dose during the manufacturing process, and sterilize them. Furthermore, they can be stored for a long period of time upon stabilization, and their protein encoding sequences can be easily modified by employing various DNA-manipulation techniques. Although DNA vaccinations strongly increase Th1-mediated immune responses in animals, several problems persist. One is about their weak immunogenicity in humans. To overcome this problem, various genetic adjuvants, electroporation, and prime-boost methods have been developed preclinically, which are reviewed here.

The MaxCyte® STX™ Scalable Transfection System uses a proprietary flow electroporation technology that can transfect up to 1E10 cells with target, reporter and protein expression plasmids, as well as other molecules, in less than 30 minutes. Transfected cells can be assayed immediately or cryopreserved for future use. Here we demonstrate the use of the MaxCyte STX system in coordination with Cornings ® HYPERFlask® Cell Culture Vessel to provide an efficient and economical solution for culturing large numbers of adherent CHO cells before and after transfection. The HYPERFlask Cell Culture Vessel features Cornings HYPER (High Yield PERformance) technology, which utilizes a gas permeable film to provide gas exchange between the internal culture environment and the external atmospheric environment. The unique, space-saving, 10-layer film design results in 1720 cm2 cell growth surface area, which is approximately 10 times that of a normal T-175 flask. We also show that cells transfected with a ...

Based on the theory of elasticity, some exact solutions of functionally gradient piezothermoelastic cantilevers under different coupled loadings are obtained. As an application, these solutions have been successfully used to identify the gradient piezoelectric parameter and the thermal material coefficients. Besides, some numerical results have been carried out for the cantilever under two different kinds of loadings. It is found that the tip deflection of the cantilever agrees very well with the experimental and theoretical findings provided by other investigations. The present study also shows that the linear change of thermal material parameters does not influence the distribution of the stress and induction of the cantilever. But it influences the components of strain and electric field strength as well as the displacement and electric potential of the cantilever. The analytical expressions have been derived for the through thickness stresses of a composite active FGM beam subjected to ...

INNOLEC_II The use of non-self-sustained Townsend discharge device in the studies of reduced electric field strength dependence of plasma ...

Features :. 100% brand new and high quality. Size:Medium. Application:Neck. Material:ABS. Item Type:Massage & Relaxation. Package size:22.5X20X5CM. Electric impulse frequency:0-1000Hz. Electric impulse width:20-400μs. 1. Pulses frequency:0Hz - 1000Hz. 2. Pulse width:20us -400us. 3. Power:3VDC30mA. 4. With 6 kinds of mode. 5. With 16 kinds of strength. 6. Main function: using Electronic pulse massager to relax neck. 7. Power supply: 2 x AAA dry cell Can use 7 hours continuously. Transition: 1cm=10mm=0.39inch. Please allow 1-3mm error due to manual measurement. pls make sure you do not mind before you bid.. Due to the difference between different monitors, the picture may not reflect the actual color of the item. Thank you!. Package:. 1 x Neck Massager Machine. 2 x Massager Pads. 1 x Wire. ​. Welcome for Drop Shipping. All orders ship within 24 Hour s. Kindly reminder, please use the massager with new battery. ...

Immunotherapy using T cells with redirected specificities has favored strategies that lead to the production of cells with stable expression of the introduced TCR. Such engineered T cells can persist in the host and establish a memory-like T cell immunity with therapeutic advantages. The expansion and long-term presence of antigen-specific T cells can, however, result in severe side effects due to off-target activation of T cells or to the massive uncontrolled lysis of the targeted cells and severe liver damage.. To reduce such safety concerns, we have previously shown that direct electroporation of mRNA coding HBV-specific TCR allows the production of large quantities of TCR-redirected T cells expressing the introduced TCR only transiently (24). Although the functional efficiency of such cells is limited to 72 to 96 hours, mRNA TCR-redirected T cells were shown to possess antitumor activity in a xenograft model of HCC (24).. In this study, we tested the antiviral efficacy of mRNA ...

Definitions  Sanitation: Reduction of the microbial population to levels considered safe by public health standards  Antiseptic: A mild disinfectant agent suitable for use on skin surfaces  -cidal: A suffix meaning that

The Flat Electrode can be used for both electroporation and electro cell fusion. The Flat Electrode generates either a divergent or homogeneous field depending on the orientation of the grooved electrodes.

Kim, Y.H., Han, K.S., Oh, s., You, S. and S.H. Kim (2005) Optimization of technical conditions for the transformation of Lactobacillus acidophilus strains by electroporation. Journal of Applied Microbiology 99: 167- ...

An arrayed collection of siRNA reagents for RNAi screening, targeting human F-box and SOCS box E3 ubiquitin ligases. Accell siRNA permits RNAi screening in difficult-to-transfect cells with no transfection reagent, virus, or electroporation.

Common problems hindering speedy progress in Seed Sciences include mobile, tissue and entire organism complexity, and notably the advanced of genomic redundancy affecting basic genetics in higher plants. not merely seed science, but mammalian biology7 also. This example features how speedy experimentation in a straightforward eukaryote in the green lineage can speed up research in more technical organisms by producing testable hypotheses using methods technically feasible only in this background of reduced complexity. Knowledge of a genome and the possibility to modify genes are essential tools in any model species. Genomic1, Transcriptomic8, and Proteomic9 information for this species is usually freely available, whereas the previously reported methods6, 10 to genetically transform are known to few laboratories worldwide. In this article, the experimental methods to genetically transform this novel model organism with an overexpression construct by means of electroporation are layed out in ...

This line was generated by the group of Adrian Bird. Exon 2 of the Mbd2 gene was replaced with the promoterless beta-geo cassette. The resulting transcript can encode the N-terminal 183 amino acids of MBD2, but translation then stops in the middle of the methyl-CpG binding domain. Gene targeting was performed in the ES cell line E14 TG2a, which is derived from the mouse substrain 129/Ola. ES cells were transfected with the linearized targeting vector by electroporation. Targeted ES cells were injected into C57BL/6 blastocysts. Chimeric pups were mated with C57BL/6 mice. MBD2 is an eagle-i resource of type Mus musculus at The University of Pennsylvania.

Martina Sonego is the author of these articles in the Journal of Visualized Experiments: Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro, In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

The gene trap vector is introduced into ES cells by electroporation or retroviral infection and clones containing gene trap insertions are selected by antibiotic resistance. Antibiotic resistant clones are then picked into 96-well master plates, which are later replicated to generate frozen stocks for long-term storage of clones, cell lysates for RNA and DNA isolation followed by PCR-based gene identification techniques.. ...

While glycerol in the electroporation buffer is typically used to make frozen competent cells, this is not advised and will lead to decreased competency. Instead the glycerol addition is strictly to increase to competency of the cells. The reason behind this correlation is not understood ...

Pañella C, Castellví Q, Moll X, Quesada R, Villanueva A, Iglesias M, Naranjo D, Sánchez-Velázquez P, Andaluz A, Grande L, et al. Focused Transhepatic Electroporation Mediated by Hypersaline Infusion throuth the Portal Vein in Rat Model. Preliminary Results on Differential Conductivity. Radiology and Oncology. 2017 ;51(4):415-421. ...

Castellví Q, Sánchez-Velázquez P, Berjano E, Burdío F, Ivorra A. Selective Electroporation of Liver Tumor Nodules by Means of Hypersaline Infusion: A Feasibility Study. In: 6th European Conference of the International Federation for Medical and Biological Engineering. Vol. 45. 6th European Conference of the International Federation for Medical and Biological Engineering. Springer International Publishing; 2015. pp. 821-824. Available from: http://dx.doi.org/10.1007/978-3-319-11128-5_204 ...