Other Inquire More and Share Questions If Any Before the Purchase on This Report At: www.absolutereports.com/enquiry/pre-order-enquiry/13903159 This report also splits the market by region: Americas- United States, Canada, Mexico, Brazil, APAC, China, Japan, Korea, Southeast Asia, India, Australia, Europe, Germany, France, UK, Italy, Russia, Spain, Middle East & Africa, Egypt, South Africa, Israel, Turkey, GCC Countries. Detailed TOC of Global Irreversible Electroporation Ablators Market Growth 2019-2024. 1 Scope of the Report. 1.1 Market Introduction. 1.2 Research Objectives. 1.3 Years Considered. 1.4 Market Research Methodology. 1.5 Economic Indicators. 1.6 Currency Considered. 2 Executive Summary. 2.1 World Market Overview. 2.2 Irreversible Electroporation Ablators Segment by Type. 2.3 Irreversible Electroporation Ablators Consumption by Type. 2.4 Irreversible Electroporation Ablators Segment by Application. 2.5 Irreversible Electroporation Ablators Consumption by Application. 3 Global ...
Global Irreversible Electroporation Ablators Market 2020 by Manufacturers, Type and Application, Forecast to 2025 is currently an appended report by MarketQuest.biz that will help you make informed decisions, know opportunities, plan new projects, explore drivers and restraints, plan effective business strategies, and provides a vision on the industry forecast. The report targets the major aspects related to global Irreversible Electroporation Ablators market growth, development plan, and focuses on significant tactics. The market has experienced an amazing change structure-wise such as product developments, launches, and trends. The market is evaluated on the basis of segments including types and applications. It demonstrates the market size, market share, market trends, and development rate. The report analyzes the progress of this market movement of significant players in this industry. Scope of the Global Irreversible Electroporation Ablators Industry:. The market report provides an ...
A new method for the ablation of undesirable tissue such as cells of a cancerous or non-cancerous tumor is disclosed. It involves the placement of electrodes into or near the vicinity of the undesirable tissue through the application of electrical pulses causing irreversible electroporation of the cells throughout the entire area of the undesirable tissue. The electric pulses irreversibly permeate the cell membranes, thereby invoking cell death. The irreversibly permeabilized cells are left in situ and are removed by the body immune system. The amount of tissue ablation achievable through the use of irreversible electroporation without inducing thermal damage is considerable.
To report on the first short-term oncologic outcomes of percutaneous irreversible electroporation for small renal masses.Patients with cT1a renal masses treated with irreversible electroporation from April 2013 through December 2016 were reviewed.
Exposing a biological cell to an electric field results in structural changes in the cell plasma membrane. If the electric field is sufficiently high, the changes in the trans-membrane voltage cause the membrane to become permeable to molecules which otherwise cannot cross it. The described phenomenon is termed electroporation [1] and can be either reversible or irreversible. If the electric field at given pulse characteristics exceeds the reversible electroporation threshold, the cell eventually returns to its normal state and survives; this is called reversible electroporation. However, if the electric field exceeds the threshold of irreversible electroporation or the cell is exposed to the electric field too long, the changes in the membrane lead to irreversible electroporation (IRE) characterized by cell death.. Both reversible and irreversible electroporation have found their use in treatment of tumors [2]. Combination of reversible electroporation and cytotoxic drugs used in chemotherapy ...
Irreversible electroporation is a minimally invasive technique for the non-thermal destruction of cells in a targeted volume of tissue, using brief electric pulses, (~100 µs long) delivered through electrodes placed into or around the targeted region. These electric pulses destabilize the integrity of the cell membrane, resulting in the creation of nanoscale defects that increase a cells permeability to exchange with its environment. When the energy of the pulses is high enough, the cell cannot recover from these effects and dies in a non-thermal manner that does not damage neighboring structures, including the extracellular matrix. IRE has been shown to spare the major vasculature, myelin sheaths, and other supporting tissues, permitting its use in proximity to these vital structures. This technique has been proposed to be harnessed as an advantageous non-thermal focal ablation technique for diseased tissues, including tumors. IRE electric pulses may be delivered through small (ø ≈ 1 mm) ...
Irreversible electroporation (IRE), a new tissue ablation procedure available since 2007, could meet the requirements for ideal focal therapy of …
PurposeNumerical simulations are used for treatment planning in clinical applications of irreversible electroporation (IRE) to determine ablation size and shape. To assess the reliability of simulations for treatment planning, we compared simulation results with empiric outcomes of renal IRE using computed tomography (CT) and histology in an animal model.MethodsThe ablation size and shape for six different IRE parameter sets (70-90 pulses, 2,000-2,700 V, 70-100 µs) for monopolar and bipolar electrodes was simulated using a numerical model. Employing these treatment parameters, 35 CT-guided IRE ablations were created in both kidneys of six pigs and followed up with CT immediately and after 24 h. Histopathology was analyzed from postablation day 1.ResultsAblation zones on CT measured 81 ± 18 % (day 0, p ≤ 0.05) and 115 ± 18 % (day 1, p ≤ 0.09) of the simulated size for monopolar electrodes, and 190 ± 33 % (day 0, p ≤ 0.001) and 234 ± 12 % (day 1, p ≤ 0.0001) for bipolar electrodes. ...
Ablation Technologies Market by Product (Radiofrequency, Ultrasound, Irreversible Electroporation, Cryotherapy, Microwave), Application (General & Cosmetic Surgery, Cardiovascular, Cancer, Ophthalmology, Orthopedics) - Global Forecasts to 2019 - Market research report and industry analysis - 8399979
Read Intracranial Nonthermal Irreversible Electroporation: InVivo Analysis, The Journal of Membrane Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Nanoknife - irreversible electroporation blijkt succesvolle operatietechniek te zijn bij lokaal uitgezaaide alvleesklierkanker. Slechts 6 patiënten (3%) kreeg recidief op 2,5 jaar meting. Mediane overleving nu al 24.9 maanden (range 4.9-85 maanden). Artikel update 8 mei 2019
mRNA electroporation of dendritic cells (DCs) facilitates processing and presentation of multiple peptides derived from whole antigen, tailored to different HLA molecules. Clinical responses to electroporated moDC vaccines, however, have been suboptimal. Human Langerhans-type DCs (LCs) are the most potent conventional DC subtype for inducing CD8+ cytotoxic T lymphocytes (CTLs) in vitro. We recently demonstrated that Wilms tumor 1 (WT1) mRNA-electroporated LCs are superior to moDCs as stimulators of tumor antigen-specific CD8+ CTLs, even though they are comparable stimulators of allogeneic T cell proliferative responses. A detailed comparative evaluation of the effects of mRNA electroporation on LCs versus moDCs, however, is needed. Immature and partially-matured human moDCs and LCs electroporated with mRNA were compared for transfection efficiency, phenotypic changes, viability, retention of transgene expression after cryopreservation, and immunogenicity. Student t test was used for each pairwise
15% on Ingenio® EZporator® Electroporation System - Valid Till Dec 31, 2019 !. The Ingenio® EZporator® Electroporation System is an easy-to-use and cost-effective electroporation system for high-efficiency transfection of mammalian cells, manufactured by Mirus Bio, USA. - PR12797768
Electroporation as a delivery method is increasingly important in gene therapy, not only in vivo but also in in vitro experimental systems. Different applications of gene electrotransfer require high viability of cells and high transfection efficiency of gene electrotransfer. It was already demonstrated that the addition of fetal bovine serum (FBS) immediately after gene electrotransfer leads to improved cell survival and transfection efficiency. Therefore, the aim of the study was to determine whether prolonged incubation of cells in FBS, for more than standard 5 min, can lead to increased transfection efficiency and improved cell survival. Different murine melanoma and murine and human endothelial cell lines were transfected with plasmid encoding green fluorescent protein and then incubated for different periods of time in FBS (5-30 min). Transfection efficiency was determined by flow cytometry and fluorescence microscopy and cell survival by cell viability assay. Prolonged incubation of cells ...
Generator Only - ECM 830 Square Wave Electroporation System, BTX - Model 45-0052 - Each : This square wave electroporation system is designed for all
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Molecular medicine through gene therapy is challenged to achieve targeted action. This is now possible utilizing bionic electrode arrays for focal delivery of naked (plasmid) DNA via gene electrotransfer. Here, we establish the properties of array-based electroporation affecting targeted gene delivery. An array with eight 300 μm platinum ring electrodes configured as a cochlear implant bionic interface was used to transduce HEK293 cell monolayers with a plasmid-DNA green fluorescent protein (GFP) reporter gene construct. Electroporation parameters were pulse intensity, number, duration, separation and electrode configuration. The latter determined the shape of the electric fields, which were mapped using a voltage probe. Electrode array-based electroporation was found to require ~100 × lower applied voltages for cell transduction than conventional electroporation. This was found to be due to compression of the field lines orthogonal to the array. A circular area of GFP-positive cells was created when
hi! is anyone out there tried electroporating Jurkat cells? If so, can you post the electroporation conditions please. cheers venkat -- Venkat Pisupati Lab -44-01223-334131 Wellcome/CRC Institute Fax -44-01223-334134 Dept. of Genetics E.mail. vnp at mole.bio.cam.ac.uk University of Cambridge UK CB2 1QR ...
This study aimed to construct DNA vaccines encoding the mouse P1A tumor antigen and to generate a protective immune response against the P815 mastocytoma, as a model for vaccines against human MAGE-type tumor antigens. DNA vaccines were constructed and delivered to mice by intramuscular electroporation before tumor challenge. Immunization with a plasmid coding for the full-length P1A significantly delayed tumor growth and mice survived at least 10 days longer than untreated controls. 10% of the mice completely rejected the P815 tumors while 50% of them showed a regression phase followed by tumor regrowth. Mice immunized by electroporation of a P1A(35-43) minigene-encoding plasmid failed to reject tumor and even delay tumor growth. The P1A(35-43)-encoding plasmid was modified and helper epitope sequences were inserted. However, these modified plasmids were not able to improve the response against P815 mastocytoma. Consistent with these results, a 12-fold higher CTL activity was observed when the plasmid
Electroporation is performed in a controlled manner in either individual or multiple biological cells or biological tissue by monitoring the electrical impedance, defined herein as the ratio of current to voltage in the electroporation cell. The impedance detects the onset of electroporation in the biological cell(s), and this information is used to control the intensity and duration of the voltage to assure that electroporation has occurred without destroying the cell(s). This is applicable to electroporation in general. In addition, a particular method and apparatus are disclosed in which electroporation and/or mass transfer across a cell membrane are accomplished by securing a cell across an opening in a barrier between two chambers such that the cell closes the opening. The barrier is either electrically insulating, impermeable to the solute, or both, depending on whether pore formation, diffusive transport of the solute across the membrane, or both are sought. Electroporation is achieved by
A method and device are herein described to treat a target region of tissue, using at least one energy delivery device coupled to a power source and positioned in a treatment position so as to irreversibly electroporate tissue to ablate a target region, and introduce regenerative materials into a treated region.
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The ECM 399 is an exponential decay wave electroporation system specifically designed to deliver the field strengths and pulse lengths required for the simple transformation of bacterial and yeast cells. In low voltage mode the ECM 399 has been used for limited transfections of mammalian cells.
Use this modular electroporation system to transfect all cell types. Preset transfection protocols for common mammalian and bacterial cells; easy programming.
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Single-cell transfection of adherent cells has been accomplished using single-cell electroporation (SCEP) with a pulled capillary. HEPES-buffered physiological saline solution containing pEGFP plasmid at a low concentration (0.16 similar to 0.78 mu g/pL) filled a 15 cm long capillary with a tip opening of 2 mu m. The electric field is applied to individual cells by bringing the tip close to the cell and subsequently applying one or two brief electric pulses. Many individual cells can thus be transfected with a small volume of plasmid-containing solution (similar to 1 mu L). The extent of electroporation is determined by measuring the percentage loss of freely diffusing thiols (chiefly reduced glutathione) that have been derivatized with the fluorogenic ThioGlo 1. A mass transport model is used to fit the time-dependent fluorescence intensity decay in the target cells. The fits, which are excellent, yield the electroporation-induced fluorescence loss at steady state and the mass transfer rate through the
The electroporation array is comprised of three technologies: microwire glass electrodes, microelectronic multiplexer stimulator chips and microfluidic flow chamber. Various substances, such as genes, gene silencing RNAi, gene inhibition agents or drugs, can be perfused into the microfluidic flow chamber. The entry of the various substances into the cells will be facilitated by electroporation. An applied electric potential causes nanoscale pores to open in the cell membrane allowing substances in the solution to freely diffuse into the cell. The specific cells selected for electroporation are defined using the computer controlled microelectronic stimulator array. An
Transgenic techniques have rapidly evolved in recent years. However, the efficiency of these techniques to produce viable offspring is still disappointingly low. The purpose of this study was to assess in vitro development, transgene expression, and integration following pronuclear or cytoplasmic microinjection of condensed or linear green fluorescent protein DNA into murine embryos using electroporation. In experiment 1, the effect of embryo orientation (group or linear) within the electroporation chamber on development was evaluated using zygotes which received one pulse duration (10 msec), and one of two voltages (250 or 400 V). Zygotes that received 400 V had the lowest development score (Group, 2.06 ? 0.12; Linear, 1.97 ? 0.13), irrespective of orientation. Embryos that received 250 V had the highest development of the voltage treated groups (Group 3.42 ? 0.12; Linear 3.32 ? 0.12), irrespective of orientation, and development was lower than the control embryos (Control 4.28 ? 0.12; Mannitol ...
RNA isolation from Xenopus inner ear sensory endorgans for transcriptional profiling and molecular cloning / Casilda Trujillo-Provencio [and others] -- Synthesis of biotin-labeled RNA for gene expression measurements using oligonucleotide arrays / Ana E. Vázquez, Liping Nie, and Ebenezer N. Yamoah -- In situ hybridization approach to study mRNA expression and distribution in cochlear frozen sections / Hakim Hiel -- Lineage analysis of inner ear cells using genomic tags for clonal identification / Takunori Satoh and Donna M. Fekete -- Genetic fate-mapping approaches : new means to explore the embryonic origins of the cochlear nucleus / Jun Chul Kim and Susan M. Dymecki -- The practical use of Cre and loxP technologies in mouse auditory research / Yiling Yu and Jian Zuo -- Helios gene gun-mediated transfection of the inner ear sensory epithelium / Inna A. Belyantseva -- Electroporation-mediated gene transfer to the developing mouse inner ear / John V. Brigande [and others] -- Isolation of ...
A study of voltage fluctuations in bilayer lipid membranes during electroporation and under current-clamp conditions is presented. Qualitative considerations based on the electroporation theory are used in order to explain the phenomenon on long time scale. Indeed, the current-clamp condition induces a feedback mechanism on the pore formation and therefore on the macroscopic conductance. Voltage fluctuations can thus be recorded. These fluctuations are nonstationary long-living and have a flicker power spectrum over nearly four decades of frequency between about 10-2 and 102Hz. The study of the fluctuations in the time domain has been performed by introducing an electrical model of the system formed by the membrane and the circuit under current-clamp configuration. The analysis of the time series gives a characteristic time of 100ms for the circuitry response to the fragments of electroporation signals with characteristic times faster than 100ms. During electroporation, the response to an ...
Serša, I.; Bajd, F.; Kranjc, M.; Busse, H.; Garnov, N.; Trampel, R.; Miklavčič, D.: Comparison of single-shot rapid acquisition with relaxation enhancement and echo planar current density MRI sequences for monitoring of electric pulse delivery in irreversible electroporation. In: 1st World congress on electroporation and pulsed electric fields in biology, medicine and food & environmental technologies, pp. 83 - 86 (Eds. Jarm, T.; Kramar, P.). Springer, Singapore (2016 ...
Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice. However, current protocols for electroporation either require the investment in specialized electroporators or corrosive pre-treatment of zygotes which compromises embryo viability. Here, we describe an easily adaptable approach for the introduction of specific mutations in C57BL/6 mice by electroporation of intact zygotes using a common electroporator with synthetic CRISPR/Cas9 components and minimal technical requirement. Direct comparison to conventional pronuclear injection demonstrates significantly reduced physical damage and thus improved embryo development with successful genome editing in up to 100% of living offspring. Hence, our novel approach for Easy Electroporation of Zygotes (EEZy) allows highly efficient generation of CRISPR/Cas9 transgenic mice while reducing the numbers of animals required.
The aim of the study is the evaluation of the ablation efficiency of the percutaneous irreversible electroporation (IRE) as primary ablation therapy of locally...
In this study, we dissolved this problem by a combination of in-vitro fertilization and electroporation techniques using CRISPR/Cas9 system (Fig. 1). We confirmed successful genome editing in in-vitro fertilized embryos without loss of survival and succeeded in the generation of Myostatin (MSTN) mutant pigs (Fig. 2). In vitro fertilization techniques in pigs has been well established. Electroporation does not require special skills and can be used to treat 40-50 embryos simultaneously, indicating that this method produces mutant pigs much more easily and quickly than previously reported. We believe that this method dramatically accelerates genome modifications in pigs in terms of the cost and techniques, and it will impact on the broad field of experimental animal research, biology, and medical science.. ...
At P1, Cells Electroporated with hNkx2.1 at E14.5 Express Lhx6 and GABA(A) The overlay image (panel on the left) and separate channels (optical sectioning panel
Electroporation is a simple yet powerful technique for breaching the cell membrane barrier. The applications of electroporation can be generally divided into two categories: the release of intracellular proteins, nucleic acids and other metabolites for analysis and the delivery of exogenous reagents
Our model of defibrillation cannot be directly scaled to the human heart. Block of conduction was evident in all of the papillary muscles in the rabbit heart, yet the larger size of human papillary muscles might prevent this effect from occurring to the same extent. It appears that the space constant is a good estimate of the size of the affected papillary muscles and bundles, which could exhibit transient block after shock. If the thickness of the fiber is comparable to the spatial depth of electroporation, then the failure of conduction is more likely to occur in thinner, compared with thicker, bundles. The latter might be electroporated less and only at the surface while the large functional core is preserved where electroporation cannot reach. Thus, we suggest that the right and left bundles, as well as the entire Purkinje system in humans, are likely to be affected by the selective blockade associated with electroporation. Clinical evidence of postshock bradycardia, asystole, and widening ...
For electroporation experiments, sgRNA was produced using the Guide-it sgRNA In Vitro Transcription Kit and combined with Guide-it Recombinant Cas9 (Electroporation-ready) to produce Cas9 RNPs. The ssDNA was produced according to the Guide-it Long ssDNA Production System Protocol-At-A-Glance. Cellartis hiPSC cells, ChiPSC 18 were electroporated using a Neon electroporation system according to the guidelines provided for each cell line. For each sample, 5 x 105 cells were electroporated with 1 µg of ssDNA or dsDNA in either the presence or absence of 2.25 µg of Cas9 nuclease combined with 0.45 µg of sgRNA. Cytotoxicity was assessed 48 hr after electroporation. Sequencing of genomic areas of interest was performed after establishing clonal lines.. The transfected cells were subjected to FACS analysis three or five days post-transfection for HEK293. Cytotoxicity analysis was performed five days post-transfection.. For the experiments with HEK293 cells, we used the Guide-it CRISPR/Cas9 System, a ...
eng] Despite the discovery of novel inhibitors of tumor angiogenesis, protein-based antiangiogenic cancer therapy suffers some limitations that antiangiogenic gene therapy could overcome. We investigated the effect on tumor growth and metastasis of three antiangiogenic plasmids administered by two intratumoral electrotransfers. Plasmids encoding recombinant disintegrin domain of ADAM-15 (RDD), thrombospondin 1 (TSP-1) and the soluble isoform of the VEGF receptor 1 (sFlt-1) were injected into B16F10 melanoma-bearing C57BL/6 mice followed by electroporation. Tumor volume was measured daily using a digital caliper. Metastasis was monitored by in vivo bioluminescence after surgical removal of the primary luciferase-encoding tumor 5 days after intratumoral electrotransfer. Markers of vascularization and cell proliferation were quantified by immunohistochemistry. All the plasmids induced a significant inhibition of tumor growth, doubling of mean survival time and long term survivors (~40% vs 0% in ...
PET scans of lung tumor near airway before (left) and 3 months after (right) irreversible electroporation treatment (credit: Constantinos Sofocleous, Memorial
CRT defibrillators receive additional? end lifesaving therapy to quickly an unusually rapid, life-threatening heart rhythm CRT and CRT-D is increasingly important therapeutic option for patients with moderate and severe heart failure since Medtronic began clinical evaluation of of its CRT systems in 1997.. About CRTIn CRT, a stopwatch? sized device chest chest and is connected by lines to the left and right ventricles of the heart. Electrical pulses, the device synchronizes heart beats, which pumps blood through the body better.. courtesy of you, the entire Kaiser Daily Health Policy view Report, search the archives, or sign up for email delivery at Kaiser Daily Health Policy Report Reprint for imperial network. A free service of The Henry J. Released. Kaiser Family Foundation. 2005 Advisory Board Company and Kaiser Family Foundation. All rights reserved.The technique of, as irreversible electroporation are known was headed by a research team headed by Boris Rubinsky, currently on leave a ...
To investigate the reliability of simulations for planning pancreatic irreversible electroporation (IRE) ablations compared with computed tomography (CT) and pathology outcomes in an animal model. Simulations were performed varying treatment parameters, including field strength (1.5-2.5 kV/cm), p...
Methods and apparatus are provided for treatment of heart arrhythmia via renal neuromodulation. Such neuromodulation may effectuate irreversible electroporation or electrofusion, ablation, necrosis a
Cell-autonomous functions of genes in the brain can be studied by inducing loss or gain of function in sparse populations of cells....
Trial Design. Pancreatic cancer resectability is defined by the absence of metastases and only limited invasion of arteries (SMA, common hepatic artery, celiac trunk) and veins (superior mesenteric vein / portal vein) (Katz et al. 2013, Ann Surg Oncol 20:2787). Only Stage III pancreatic cancer (AJCC - TNM 7th edition) will be enrolled in the study. All cases will be reviewed at the multidisciplinary meeting to confirm eligibility. After obtaining their informed consent patients will receive 4 cycles of FOLFIRINOX chemotherapy. Following reassessment, patients without progression will proceed to local treatment. Diagnostic laparoscopy will be performed to exclude distant metastases. Unresectable tumors up to 4cm will be randomized between stereotactic body radiotherapy (SBRT) (Arm A) versus irreversible electroporation (IRE) (Arm B). Tumors larger than 4cm will be randomized to chemo-radiation (Intensity modulated radiation therapy: 28 × 2Gy = 56 Gy to the primary tumor and metastatic lymph ...
nexavar online cheap taking the oral vascular endothelial growth factor receptor (VEGFR) tyrosine kinase inhibitors (TKIs) sorafenib and sunitinib for renal cell carcinoma (RCC) had a significantly elevated danger for cardiovascular events, significantly stroke, in accordance with the outcomes of a study published not too long ago in Cancer. Strati﫿cation for sufferers with hepatocellular carcinoma. Irreversible electroporation, utilizing the NanoKnife, is a new therapy for liver most cancers that is administered by radiologists. In a commentary revealed final yr, a group of physicians decried the cost of this drug, particularly for the reason that Nationwide Institutes of Well being and Cystic Fibrosis Foundation helped help its development (JAMA ...
Irreversible electroporation (IRE) is a minimally invasive tissue ablation technique which utilizes electric pulses delivered by electrodes to a targeted area of tissue to produce high amplitude electric fields, thus inducing irreversible damage to the cell membrane lipid bilayer. An important application of this technique is for cancer tissue ablation. Mathematical modelling is considered important in IRE treatment planning. In the past, IRE mathematical modelling used a deterministic single value for the amplitude of the electric field required for causing cell death. However, tissue, particularly cancerous tissue, is comprised of a population of different cells of different sizes and orientations, which in conventional IRE are exposed to complex electric fields; therefore, using a deterministic single value is overly simplistic. We introduce and describe a new methodology for evaluating IRE induced cell death in tissue. Our approach employs a statistical Peleg-Fermi model to correlate probability of
TY - JOUR. T1 - In vivo electroporation of adult mouse sensory neurons for studying peripheral axon regeneration. AU - Saijilafu, AU - Zhang, Bo Yin. AU - Zhou, Feng Quan. N1 - Publisher Copyright: © Springer Science+Business Media New York 2014. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.. PY - 2014. Y1 - 2014. N2 - Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain-and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.. AB - Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and ...
IRE was used to treat 48 tumors in 47 patients. 22/48 tumors (45.8%) were biopsy-confirmed renal cell carcinoma (RCC). No complications ≥ Clavien Grade III occurred and 36 patients (76.6%) were discharged the same day. Initial treatment success rate was 91.7% (n=44/48); 3 treatment failures were managed with salvage radiofrequency ablation and 1 with robotic partial nephrectomy. Median follow up was 50.4-months (IQR 29.0-65.5). The 5-year local recurrence-free survival was 81.4% in biopsy-confirmed RCC patients and 81.0% in all patients. 5-year metastasis-free survival was 93.3% and 97.1%, respectively, and 5-year overall survival was 92.3% and 90.6%, respectively. 5-year cancer-specific survival was 100% for both biopsy-confirmed RCC and all patient groups ...
Men who have undergone a Transurethral Resection of the Prostate (TURP) for symptomatic lower urinary tract symptoms within 6 months. These patients may be included within the trial if deferred from consenting and screening until at least 6 months following the TURP ...
The bactericidal efficiency of ultrahigh hydrostatic pressure (UMP) and pulsed electric field (PEF) were studied in combination with two acteriocins, nisin A and pediocin AcH, and lysozyme. Several Gram-positive and Gram-negative bacterial species and strains associated with food spoilage and foodborne diseases were tested. The results showed that both UHP and PEF inflict lethal and sublethal injury to Gram-positive and Gram-negative bacterial cells. As the sublethally-injured cells became sensitive to the bactericidal action of the two bacteriocins and lysozyme, the combination of the UHP and PEF treatment, along with bacteriocin and lysozyme resulted in greater viability loss than when only the UHP or PEF are used. In general, under the study conditions, UHP and bacteriocins produced more viability loss than PEF with and without bacteriocins. By combining bacteriocins and lysozyme, UHP at 30,000 psi for 1 min produced viability loss of 13.8 logs in Listeria monocytogenes Scott A and 6.3 logs in
Fingerprint Dive into the research topics of Investigating mammalian axon regeneration: In vivo electroporation of adult mouse dorsal root ganglion. Together they form a unique fingerprint. ...
http://www.ncbi.nlm.nih.gov/pubmed/19074264. Bestman and Cline used their in vivo single-cell electroporation protocol to introduce lissamine-tagged Morpholino antisense oligos into individual optic tectal neurons in stage 46 to 48 albino Xenopus laevis tadpoles. They knocked down expression of cytoplasmic polyadenylation element binding protein 1 (CPEB1), a component of some brain ribonucleoprotein (RNP) granules. CPEB1 binds to RNA that have cytoplasmic polyadenylation elements (CPE) in their 3-untranslated regions; CPEB1 is estimated by bioinformatics to bind to about 7% of brain mRNAs. Binding of CPEB1 to an mRNAs CPE represses its translation and regulates microtubule-dependant trafficking. When CPEB1 is phosphorylated, the mRNA can be polyadenylated and translated.. The authors collected time-lapse images of neurons over a period of three days after treatment with either CPEB1-targeted Morpholinos or control Morpholinos. In either treatment the dendritic arbors grew, but the arbors of ...
BSS032 at BANGOR.AC.UK Writes------------------------ Hi Netters, Has anyone tried electroporation as an alternative to in vitro packaging in order to make a lambda library? Comments, experiences, references welcomed. Jon R Sayers (Cell and Molecular Biology), Biochemistry Dept., University College North Wales, Univ of Wales, Bangor, LL57 2UW. Tel +44 248 38 23 54 Fax +44 248 370731 Email BSS032 at BANGOR.AC.UK ***************** Dear BSS032 at BANGOR.AC.UK I am in the process of doing that experiment. Ill tell you what happens tomorrow! Ill be electroporating a ligation but it will give some idea how it works. Dan Gietz ______________________________________ R.Daniel Gietz Ph.D. Assistant Professor Department of Human Genetics University of Manitoba 770 Bannatyne Ave, Rm 250 Winnipeg, Manitoba, Canada R3E 0W3 Tel.: (204)789-3458 Fax.: (204)786-8712 E-mail GIETZ at BLDGHSC.LAN1.UMANITOBA.CA Trying to do the Manitoba Thing ...
In this study an automatic system is presented to perform electroporation, also known as electropermeabilization, on adherent cells. It is an intention of this system to apply electric field pulses directly to cells growing in standard multiwell plates as a step forward to include this technique in standard laboratory protocols. An interdigitated microelectrode assembly constructed with Printed Circuit Board (PCB) is placed closely above the cell monolayer, and in order to avoid direct contact with cells, small micro-separators were included in the structure. Additionally, distribution of current density was modified by filling the gap between adjacent electrodes with a non conductive material as predicted by electric field simulations. This modification helps to concentrate the electric field intensity in the region where cells are present. The device was tested using C2C12 cell line growing adhered in 24 multiwell plates and fluorescent labeled dextran FD20S as the molecule to be delivered. ...
A group of researchers from Tel Aviv University and Harvard University is proposing a new non-invasive method to prevent burn-related hypertrophic scars - raised tissue caused by excessive collagen - using short, pulsed electric fields.. People dont die from scars, but they do suffer from them, said Dr. Alexander Golberg of TAUs Porter School of Environmental Studies. We believe that the technology we developed, called partial irreversible electroporation (pIRE), can be used to prevent debilitating burn scars from forming.. According to the World Health Organization, 10 percent of all unintentional-injury deaths result from burns. But even for those who survive the destruction of skin and tissue cells, post-burn scarring creates lifelong physical, psychological and social suffering.. The experimental pIRE technique harnesses microsecond-pulsed, high-voltage, non-thermal electric fields to control the bodys natural response to trauma - the proliferation of collagen cells that cause ...
Irreversible electroporation, Liver resection, Minimally invasive pancreas surgery, Small bowel resection, Soft tissue ...tumor ablation, Cholecystectomy, Liver cyst removal, Laparoscopic surgery, Liver cyst fenestration, Cryoablation for cancer, Cancer treatment, Soft tissue sarcoma surgery, Bile duct stone removal, Splenectomy, Microwave ablation for cancer, Radiofrequency ablation for cancer, Pancreatectomy, Gastrectomy, Hepatobiliary disease evaluation, Hepatobiliary disease postoperative care, Portal hypertension management, Paracentesis, Pancreatic enucleation, Minimally invasive surgery, Minimally invasive intestinal surgery, Minimally invasive liver surgery, Whipple procedure, Liver tumor ablation, Bile duct resection, Bile duct injury, Polycystic liver disease, Peritoneal cancer, Idiopathic thrombocytopenic purpura, Bile duct stone, Pancreatic cancer, Pancreatitis, Biliary obstruction, Retroperitoneal sarcoma, Liver hemangioma, Pancreatic cyst, Small bowel cancer, Liver tumor, Bile duct ...
Background: Irreversible electroporation (IRE) is a local ablation technique utilizing high voltage, low energy direct current to create nanopores in cell membrane which disrupt homeostasis and leads to cell death. Previous reports have suggested IRE may have a role in treating borderline resectable and unresectable Stage 3 pancreatic tumors. Methods: Patients with Stage 3 pancreatic ductal adenocarcinoma (PDAC) will be enrolled in either a randomized, controlled, multicenter trial (RCT) or a multicenter registry study. Subjects enrolled in the RCT must have no evidence of disease progression after 3 months of modified FOLFIRINOX (mFOLFIRINOX) treatment prior to being randomization to either a control or IRE arm. Post-induction and post-IRE treatment for the control and IRE arms, respectively, will be left to the discretion of the treating physician. The RCT will enroll 528 subjects with 264 per arm and include up to 15 sites. All subjects will be followed for at least 24 months or until death. The
Pulsed electric current can be used to produce irreversible electroporation (IRE) of cell membranes with resulting cell death. This process has been shown to ablate tumors in animal and human studies. A pulsating direct current of 20 to 50 A and 500 to 3000 V is delivered into metastatic or primary tumors in the liver, kidney, or lung via needle electrodes inserted under computed tomography (CT) or ultrasound guidance. Patients usually require general anesthesia with muscle relaxant. Currently, circa 30 procedures have been done at our institution with good or excellent results. However, several patients have had severe pain postoperatively.. Dexmedetomidine is an α2-adrenoreceptor agonist with sedative, analgesic and anxiolytic effects, and it has more selective α2-adrenergic effect than clonidine. We will evaluate perioperative dexmedetomidine 0.4 µg/kg/hr infusion effects on hemodynamics, anesthetic consumption, and recovery profiles during anesthesia for IRE of solid organs tumours. ...
SPARED Registry. This registry represents a multi-institutional effort to prospectively obtain real-world clinical data on prostate-sparing ablative devices including high-intensity focused ultrasound (HIFU), cryotherapy, focal laser ablation, irreversible electroporation, photodynamic therapy, and future technologies.. Background of the SPARED Registry. In light of the rapid adoption of novel but unproven technologies for prostate ablation, the need to monitor the safety and effectiveness in the post-market arena is essential. Following the recent approval of high-intensity focused ultrasound (HIFU) for ablation of prostate tissue, it is expected that more companies with novel technologies will apply for FDA clearance/approval. In light of the ever-expanding non-surgical options to treat prostate cancer, the MDEpiNet Science and Infrastructure Center at Weill Cornell, in collaboration with a multi-disciplinary group of stakeholders, has initiated the Study of Prostate Ablation Related Energy ...
European network for development of electroporation-based technologies and treatments. This COST Action aims at fostering development of new and existing electroporation-based applications and as well as facilitating coordination and interdisciplinary exchange of knowledge and know-how between researchers from different scientific fields and countries. Action results will provide scientific and technological progress in the field of medicine, biotechnology and environment preservation.
Gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study Zohre Rezaee,1,2 Ali Yadollahpour,1,2 Vahid Bayati,1 Fereshteh Negad Dehbashi1 1Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Avhaz, Iran; 2Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Background and objective: Radiation therapy (RT) is the gold standard treatment for more than half of known tumors. Despite recent improvements in RT efficiency, the side effects of ionizing radiation (IR) in normal tissues are a dose-limiting factor that restricts higher doses in tumor treatment. One approach to enhance the efficiency of RT is the application of radiosensitizers to selectively increase the dose at the tumor site. Gold nanoparticles (GNPs) and electroporation (EP) have shown good potential as radiosensitizers for RT. This study aims to
A device for manipulating a molecule in vivo relative to a target tissue in three dimensions includes a support and at least one member affixed to and extending away from the support. The member has at least two discrete and separately activatable electrodes. The electrodes are configured to establish a first electromagnetic field between selected electrodes sufficient to manipulate a molecule relative to a target tissue. The electrodes are further configured to establish a second, typically higher-level, electromagnetic field sufficient to cause transient permeability of a cell membrane within the target tissue. A third electromagnetic field may also be applied to cause further translation of the molecule into an electropermeabilized cell and/or manipulated with respect to the tissue. Thus three-dimensional manipulation of the molecule relative to the target tissue is effected to optimize a desired positioning thereof, such as entry into a cell.
0100]In a further improvement to the device of FIG. 1 (as shown in FIGS. 2a to 2c), means are provided to sense when during insertion the needles 6 are at the correct depth in the muscle or body tissue for injection of the DNA to begin and to automatically move the pistons 12 to effect the injection. These means comprise a moveable skin contact 20 which contacts the skin S as shown in FIGS. 2a to c. As the needles 6 are inserted into the muscle or body tissue to be treated, the contact 20 is pushed upwardly towards the support member 4. The contact member 20 is attached to a lever mechanism consisting of a substantially vertical link 22 extending upwardly from the contact member 20 and a lever 24 which is attached at a first end to the vertical link 22. The lever 24 is attached at its other end to means 26 for causing the pistons 12 to move downwardly. The lever is adapted to pivot about a point 28 on the support member 4 located between the two ends of the lever 24. Thus, as the contact 20 ...
DNA vaccination holds great promise for the prevention and treatment of cancer and infectious diseases. However, the clinical ability of DNA vaccines is still controversial due to the limited immune response initially observed in humans. We hypothesized that electroporation of a plasmid encoding the HIV-1 Gag viral capsid protein would enhance cancer DNA vaccine potency. DNA electroporation used to deliver plasmids in vivo, induced type I interferons, thereby supporting the activation of innate immunity. The coadministration of ovalbumin (OVA) and HIV-1 Gag encoding plasmids modulated the adaptive immune response. This strategy favored antigen-specific Th1 immunity, delayed B16F10-OVA tumor growth and improved mouse survival in both prophylactic and therapeutic vaccination approaches. Similarly, a prophylactic DNA immunization against the melanoma-associated antigen gp100 was enhanced by the codelivery of the HIV-1 Gag plasmid. The adjuvant effect was not driven by the formation of HIV-1 Gag ...
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p,Use the 0.2 cm–gap Gene Pulser/MicroPulser Electroporation Cuvettes for reproducible transformation of bacteria, yeast, and mammalian cells, including primary and stem cells. These high-quality cuvettes are compatible with most electroporation systems, including Bio-Rad’s Gene Pulser MXcell™, Gene Pulser Xcell™, and MicroPulser Electroporators.,/p, ,p class=descHeader,,a href=/en-us/product/gene-pulser-micropulser-electroporation-cuvettes,Gene Pulser/MicroPulser Cuvettes,/a, are available in three gap widths:,/p, ,ul, ,li,0.1 cm (brown cap) – for bacteria and yeast,/li, ,li,0.2 cm (green cap) – for bacteria, yeast, and mammalian cells, including primary and stem cells,/li, ,li,0.4 cm (blue cap) – for mammalian cells, including primary and stem cells, or plant protoplasts,/li, ,/ul, ,h2 class=descHeader,Packaging Options,/h2, ,table class=pd_table pd_gridlines border=0, ,tbody, ,tr class=pd_colorbackground, ,td class=pd_tableheader ...
Cells transfected with 8 pmol of luc2-encoding IVT RNA were harvested at different time points to measure luminescence from 1 × 104 cells (Bright-Glo Luciferase Assay Kit for 96-well plates (Promega)). Results are the mean ± SD luminescence. Percent luminescence is relative to the highest luminescence signal obtained in each experiment. (c) Viability, reporter gene expression of iDCs and mDCs after eGFP and luc2 electroporation and phenotype after electroporation are depicted in descending order, respectively. iDCs (left panel) and mDCs (right panel) of 2 different donors were transfected with 10 μg eGFP- or luc2-encoding IVT RNA. Negative controls: cells electroporated without RNA (mock) and unelectroporated (no ep) cells. Cells were harvested at different time points. Viability and HLA-DR, CD83, and eGFP expression levels were determined by flow cytometry. Luciferase activity of 1 × 104 viable cells was measured by luminescence in triplicate ...
A system for treating benign prostate hyperplasia (BPH) of a prostate. At least first and second mono-polar electrodes are configured to be introduced at or near a BPH tissue site of the prostate gland of the patient. A voltage pulse generator is coupled to the first and second mono-polar electrodes. The voltage pulse generator is configured to apply sufficient electrical pulses between the first and second mono-polar electrodes to induce electroporation of cells in the BPH tissue site, to create necrosis of cells of the BPH tissue site, but insufficient to create a thermal damaging effect to a majority of the BPH tissue site.
Iam facing a problem with cloning. Iam using the pGEM T easy vector for cloning. Followed the ligation protocol as per promega instuctions. I tried to transform the ligated vector into E.coli DH5 Alpha by electroporation but got no results. Promega has provide with a positive control insert along with the kit. when the electroporated cells of ecoli were plated got no white colonies nor in the +ve control nor in negative control (only with the pGEM vector alone, no blue colonies). Thot the cells were not competent and went for calcium chloride method of transformation using Ecoli JM109 this time we also used pUC18 as a negative control besides the pGEM vector alone. The trasformation with pUC gave blue colonies but no blue colonies in the pGEM vector alone and no white colonies in +ve control promega provided nor in our insert ...
Altogen Biosystems provides in vivo transfection reagents, over 100 pre-optimized in vitro transfection kits for cell lines and primary cells, and electroporation delivery products. Transfection reagents are highly efficient for DNA and siRNA transfection in vivo and in vitro. Altogen CRO offers in vivo RNAi services, tumor xenograft models, toxicology testing, stable cell line generation, and cell banking cryopreservation services
Altogen Biosystems provides in vivo transfection reagents, over 100 pre-optimized in vitro transfection kits for cell lines and primary cells, and electroporation delivery products. Transfection reagents are highly efficient for DNA and siRNA transfection in vivo and in vitro. Altogen CRO offers in vivo RNAi services, tumor xenograft models, toxicology testing, stable cell line generation, and cell banking cryopreservation services
Safety and comparative immunogenicity of an HIV-1 DNA vaccine in combination with plasmid interleukin 12 and impact of intramuscular electroporation for delivery. J Infect Dis. 2013 Sep 01; 208(5):818-29 ...
BACKGROUND: Electrochemotherapy describes the use of electric pulses to enhance chemotherapy uptake, and has proven highly efficient in treating cutaneous metastases. Patients referred for electrochemotherapy present with diverse clinical pictures, from multiple small lesions to large, ulcerated lesions. Post-electrochemotherapy pain has been observed in some patients. The objectives of this study were to evaluate pain scores before and after electrochemotherapy, and to investigate if patients at risk of post-procedure pain could be identified. METHODS: Seven cancer centres in the International Network for Sharing Practices on Electrochemotherapy (INSPECT) consecutively and prospectively reported to a common database. Electrochemotherapy consisted of intratumoural or intravenous injection of bleomycin, followed by delivery of electric pulses in local or general anesthesia. RESULTS: Of 121 patients 39% had metastatic melanoma, 18% squamous cell carcinoma, 16% breast cancer, 13% basal-cell ...
A new method that we have called oligosaccharide mapping is described for the analysis of radiolabelled heparan sulphate and other glycosaminoglycans. The method involves specific enzymic or chemical scission of polysaccharide chains followed by high-resolution separation of the degradation products by polyacrylamide-gradient-gel electrophoresis. The separated oligosaccharides are immobilized on charged nylon membranes by electrotransfer and detected by fluorography. A complex pattern of discrete bands is observed covering an oligosaccharide size range from degree of polymerization (d.p.) 2 (disaccharide) to approximately d.p. 40. Separation is due principally to differences in Mr, though the method also seems to detect variations in conformation of oligosaccharide isomers. Resolution of oligosaccharides is superior to that obtained with isocratic polyacrylamide-gel-electrophoresis systems or gel chromatography, and reveals structural details that are not accessible by other methods. For ...
The skin is an attractive tissue for vaccination in a clinical setting due to the accessibility of the target, the ease of monitoring and most importantly the immune competent nature of the dermal tissue. While skin electroporation offers an exciting and novel future methodology for the delivery of DNA vaccines in the clinic, little is known about the actual mechanism of the approach and the elucidation of the resulting immune responses. To further understand the mechanism of this platform, the expression kinetics and localization of a reporter plasmid delivered via a surface dermal electroporation (SEP) device as well as the effect that this treatment would have on the resident immune cells in that tissue was investigated. Initially a time course (day 0 to day 21) of enhanced gene delivery with electroporation (EP) was performed to observe the localization of green fluorescent protein (GFP) expression and the kinetics of its appearance as well as clearance. Using gross imaging, GFP expression was not
Neural stem cells are cells in the developing nervous system that have the capacity to give rise to daughter cells that can mature, or differentiate into various different cell types. In the midbrain region of a developing embryo, a type of neural stem cells called floor plate cells have been shown to give rise to dopamine neurons.. Nato3, a basic helix-loop-helix protein, is expressed in the floor plate region of the midbrain in the developing embryo. To determine if Nato3 expression is sufficient to promote floor plate cell differentiation into dopamine neuron in the developing neural tube we are misexpressing Nato3 in the neural stem cells of the hindbrain and midbrain using in ovo electroporation. We are monitoring neural progenitors and their progeny that misexpress the electroporated Nato3 during development using a bicistronic EGFP reported expression vector.. Using immunohistochemistry we are comparing the effect of Nato3 misexpression on neural stem cells in the hindbrain and midbrain ...
This is the readme for the model associated with the paper: Berzhanskaya J, Chernyy N, Gluckman BJ, Schiff SJ, Ascoli GA (2013) Modulation of hippocampal rhythms by subthreshold electric fields and network topology. J Comput Neurosci 34(3):369-389 This is the code that the paper authors used. This NEURON model simulates OPb network made of 20 Pyramidal, Oriens and Basket cells (60 total). Biophysically realistic Pyramidal cells have full 3D morphological structure, while Oriens and Basket cells have simplfied compartmental structure. Synaptic weight distribution on Pyramidal cells is modeled after Li and Ascoli (2006, 2008). Experimental data on the external electric field effects at the single cell level are collected using control circuits described in Gluckman et al. (1996, 2001). External electric field application is simulated after C. McIntyre and M. Robertson. Usage instructions: ------------------- Either auto-launch from ModelDB or download, extract the archive and compile the mod files ...
Dry ageing of red meat is a re-emerging technique and product demand has increased considerably in recent years. Dry ageing of meat generates unique flavours and adds value. Most dry ageing is done on premium, highly marbled beef. With all its popularity, minimal research has been done on dry ageing of lean, low fat content animal models. In this research, the main focus was to study dry ageing in two lean red meat models, venison and bull beef with fast and slow oxidation, respectively. The two studies investigated the application of novel technology to enhance and add value to some low value cuts from lean bull beef.. The first research project applied pulsed electric field (PEF), a standalone non-thermal technology, with the aim of improving meat quality attributes and enhanced moisture transfer in the dry ageing process. The results across the two trials showed that High PEF (HPEF-10 kV, 50 Hz, 20 µs) treatment had sufficient pulsed electrical field strength to cause cell electroporation; ...
Conversely, to test the effect of ARX overexpression on cell proliferation, we introduced an ARX-overexpressing construct together with the DsRed vector in cortical progenitor cells using in utero electroporation. Contrary to ARX inactivation, there were substantially more DsRed-positive cells lining the ventricle 3 d after electroporation with CAG-IRES-EGFP-ARX compared with control (Fig. 2E,F). To establish whether these cells were still proliferating, we labeled sections with P-H3 and Ki67 antibodies (Fig. 2G,H). Whereas Ki67 is a marker for proliferating cells from S-phase through M-phase of the cell cycle, P-H3 only labels cells that are in late G2- or M-phase. When the control plasmid was introduced, ∼8.6 ± 2% of DsRed-labeled cells in the VZ/SVZ were P-H3 positive (68 cells/793 DsRed-positive cells), and 10.4 ± 5.1% were Ki67 positive (14 cells/135 DsRed-positive cells). On the contrary, when ARX was overexpressed, only 2 ± 0.8% of the DsRed-positive cells in the VZ/SVZ were P-H3 ...
Spanggaard I., Snoj M., Cavalcanti A., Bouquet C., Sersa G., Robert C., Cemazar M., Dam E., Vasseur B., Attali P., Mir L.M., Gehl J. Gene electrotransfer of plasmid antiangiogenic metargidin peptide (AMEP) in disseminated melanoma: safety and efficacy results of a phase I first-in-man study. Human Gene Ther Clin Developme, 2013; 24: 99-107.[PubMed: 23980876 ...
The device and process of this invention provide for eliminating reading errors caused by over-erased cells by applying flash erasing pulses, then flash programming pulses to the cells of an EEPROM array. The flash erasing pulses are sufficient in strength to over-erase the cells. The flash programming pulses applied to the control gates have the same voltages as those used to program individual cells. The strength of the programming electric field pulses adjacent the floating gates is controlled by applying a biasing voltage to one of the source/drain regions of the cells. The biasing voltage controls the energy level of the programming field pulses such that only enough charge is transferred to the floating gates to cause the threshold voltages of the cells to have positive values less than that of a predetermined wordline select voltage.
Radical surgery is the clinical standard for the treatment of non-metastatic cancer in the genitourinary tract. While the patient is cured of their disease, surgery imposes loss of urinary and sexual function, reduction in the quality of life, and increases life-long risk of complications. My lab addresses this critical challenge by designing new medical devices and developing technology for minimally-invasive, image-guided, non-surgical cancer therapy, with specific emphasis on the preservation of organ function following treatment. We study the biological effects of electric pulses on the barrier function of cell membranes, permeability of endothelium, and the integrity of the extracellular matrix. We use mathematical models and computer simulations to optimize electric pulse parameters for tissue ablation without concomitant heating, improve the specificity of lethal effects to specific cell types, and for the disruption of biological barriers to promote drug delivery. These findings are then ...
Best transfection method/reagent for HeLa cells? - posted in Cell Biology: Im planning to do some transient transfection of HeLa cells. Aim is to do some CoIP and localization studies. I asked different fellows, some say Fugene is the best, some proposed TFX while other said, this is all crap, use Lipofectamine. Now im a little bit puzzled, which one i should buy... Which transfection method / reagent would you recommend?
The nitric oxide synthase 1 adaptor protein (NOS1AP) is an adaptor protein implicated in a number of human conditions including schizophrenia, anxiety and cardiac QT syndrome. Previous studies have shown that NOS1AP and some of its isoforms associate with the tumor suppressor protein scribble. Since scribble has been linked to the Hippo pathway, I set out to determine if NOS1AP associates with the Hippo pathway and whether it controls aspects of neuronal development. Here I show that NOS1AP and NOS1APc interact with the transcriptional co-activator yes-associated protein (YAP), a component of the Hippo cascade. Further both NOS1APa and NOS1APc show partial co-distribute with YAP in HEK293Tcells, with NOS1APc having better co-distribution. In situ hybridization and immunocytochemistry studies reveal that NOS1APc is expressed in the developing spinal cord. NOS1APc is expressed in the floor plate and roof plate and shows a similar profile to radial glial cells. In ovo electroporation of cDNA ...