Profacgen offers professional Electrophoretic Mobility Shift Assay (EMSA) service to help your study on DNA-protein interaction, RNA-protein interaction, and even DNA-RNA interaction.
Capillary electrophoretic mobility shift assay for binding of DNA with NFAT3, a transcription factor from H9c2 cardiac myoblast cells ...
TY - JOUR. T1 - Protein-deoxyribonucleic acid interactions linked to gene expression. T2 - electrophoretic mobility shift assay.. AU - Javed, Amjad. AU - Zaidi, Sayyed K.. AU - Gutierrez, Soraya E.. AU - Lengner, Christopher J.. AU - Harrington, Kimberly S.. AU - Hovhannisyan, Hayk. AU - Cho, Brian C.. AU - Pratap, Jitesh. AU - Pockwinse, Shirwin M.. AU - Montecino, Martin. AU - van Wijnen, André J.. AU - Lian, Jane B.. AU - Stein, Janet L.. AU - Stein, Gary S.. PY - 2004. Y1 - 2004. UR - http://www.scopus.com/inward/record.url?scp=5444248659&partnerID=8YFLogxK. M3 - Article. C2 - 15269397. AN - SCOPUS:5444248659. VL - 285. SP - 45. EP - 55. JO - Methods in molecular biology (Clifton, N.J.). JF - Methods in molecular biology (Clifton, N.J.). SN - 1064-3745. ER - ...
Die elektrophoretische Mobilität Shift-Assay (EMSA) ist eine biochemische Verfahren zur Bindung zwischen Proteinen und Nukleinsäuren zu erhellen. In dieser...
هنا نقدم بروتوكول لتحليل التفاعلات RNA / البروتين. ويستند هذا التحول فحص التنقل الكهربي (EMSA) ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
In article ,39t6d7$ja2 at nuscc.nus.sg,, sci30828 at leonis.nus.sg (C G PADMASHREE) writes: ,I am an inexperienced undergraduate who has been assigned a project on ,gel mobility shift assays involving binding of steroid hormones to their ,receptors.I request for help to find suitable references on this ,subject.Early help would be greatly appreciated.Thank you. , Try a reference availablr in most molecular biology labs: Ausubel et.al. (eds) Current protocols in Molecular Biology (Red Book) Volume 2, Chapter 12 DNA-Protein interactions Emmanuel ...
Kyle Tamshen, Robert Kousnetsov, and Amanda Dewey use electrophoretic mobility shift assays to analyze the interactions between protein LIN-31 and DNA from lin-39.. ...
Event Electromobility & Low Carbon Vehicles 2016-17. This course provides an understanding of the policy background and principles behind the concept of electromobility. Youll gain an understanding of the latest changes in research, legislation, ...
Furthermore, the examination of DNA-bound FOXO3a by electrophoretic mobility-shift assay demonstrated a marked increase in nuclear, DNA bound FOXO3a in GK rats with MI compared to Sham-GK and corresponding post-MI Wistar controls (Figure 5D). ...
This kit is not sensitive enough for a complex mixuture of protein and RNA. This kit has most optimum results when used with a more purified sample. The analysis of a complex solution with low concentrations of the actual target molecule requires more sensitivity than fluorescence can provide. From Molecular Probes technical assistance ...
Gel shift assay: (aka gel mobility shift assay (GMSA), band shift assay (BSA), electrophoretic mobility shift assay (EMSA)) A method by which one can determine whether a particular protein preparation contains factors which bind to a particular DNA fragment. When a radiolabeled DNA fragment is run on a gel, it shows a characteristic mobility. If it is first incubated with a cellular extract of proteins (or with purified protein), any protein-DNA complexes will migrate slower than the naked DNA - a shifted band.. Gene: A unit of DNA which performs one function. Usually, this is equated with the production of one RNA or one protein. A gene contains coding regions, introns, untranslated regions and control regions.. Genome: The total DNA contained in each cell of an organism. Mammalian genomic DNA (including that of humans) contains 6x109 base pairs of DNA per diploid cell. There are somewhere in the order of a hundred thousand genes, including coding regions, 5 and 3 untranslated regions, ...
DNA binding capacity of Orf8 and Orf16 by electrophoretic mobility shift assays (EMSAs). Preparation of DNA substrate is graphically shown in panel A. US8 and U
Separation of antigens of low electrophoretic mobility by the method of immunofiltration 的翻译是:采用免疫渗滤法分离电泳迁移率较低的抗原 是什么意思?英文翻译中文,中文翻译英文,怎么说?-我要翻译网
Gentaur molecular products has all kinds of products like :search , Signosis 2011 \ GAS_ISRE EMSA Kit \ GS-0017 for more molecular products just contact us
Mobility & Seniors - |p|There is nothing nicer than seeing your horse or pony striding out soundly whether they are five or twenty five, but there may be periods when their mobility is not so good and this can be influenced by a number
Yes folks, it does not get any easier - to resolve your dogs mobility issues. Its all-natural, with money-back guarantee. Better than 90% success rate.
888-609-0242 Worried you or a loved one developed an NTM Infection after Heart Surgery in Rossville TN? Call us anytime 24/7 for a Free Case Evaluation... No Fee Unless You Win!
Hypocrea jecorina (anamorph Trichoderma reesei) is a filamentous ascomycete of industrial importance due to its hydrolases (e.g., xylanases and cellulases). The regulation of gene expression can influence the composition of the hydrolase cocktail, and thus, transcription factors are a major target of current research. Here, we design an approach for identifying a repressor of a xylanase-encoding gene. We used streptavidin affinity chromatography to isolate the Xylanase promoter-binding protein 1 (Xpp1). The optimal conditions and templates for the chromatography step were chosen according to the results of an electrophoretic mobility shift assay performed under repressing conditions, which yielded a DNA-protein complex specific to the AGAA-box (the previously identified, tetranucleotide cis-acting element). After isolating AGAA-box binding proteins, the eluted proteins were identified with Nano-HPLC/tandem MS-coupled detection. We compared the identified peptides to sequences in the H. jecorina genome
ERRα interacts with the mouse Sirt3 promoter in vitro and in vivo.A, Electrophoretic mobility shift assay was executed using a biotin probe. Biotin-labeled dou
Whether you purchased your mobility product from us or not, SpinLife® can help you locate and purchase the exact part you need! ...
EMSA troubleshooting - posted in Molecular Biology: Hi All, I am trying to optimize an EMSA to look at DNA binding by NF-kB. I started with 293T cells treated with TNF for either 30 or 60 minutes. I extracted the nuclear fraction using high salt buffer (420mM) with glycerol and checked for cytoplasmic contamination by western prior to performing the EMSA. The protein concentration of each sample was normalized using high salt buffer (i.e. the salt concentration in the binding reacti...
Gentaur molecular products has all kinds of products like :search , Panomics \ ERE EMSA Kit \ AY1012 for more molecular products just contact us
Confused by the difference between mobility work & stretching? Learn how to properly structure a mobility plan and increase your performance.
As people age, they might suffer mobility issues. Discover how a proactive approach to improving mobility can prevent future complications in the Elderly.
Global Mobility - For almost two decades, Deloitte has taken a leadership role in advancing the World Economic Forums objective of
Global Mobility - For almost two decades, Deloitte has taken a leadership role in advancing the World Economic Forums objective of
FT is a complex trait with polygenic inheritance. While the genetic basis of FT has been widely studied in cereals by bi-parental linkage mapping and expression profiling, exploitation of the allelic and phenotypic variation of FT in rye by association studies has lagged behind [20, 21, 24]. This study reports the first candidate gene-based association studies in rye examining the genetic basis of FT.. Statistically significant SNP-FT associations were identified in nine candidate genes hypothesized to be involved in the frost responsive network among which the transcription factor Ice2 is one of the key factors. The function of Ice2 was characterized both in wheat and Arabidopsis [25, 26]. Over-expression of TaIce2 and AtIce2 in transgenic Arabidopsis plants resulted in increased FT of transgenic plants and was associated with higher expression levels of the Cbf gene family. Using electrophoresis mobility shift assays, Badawi et al. [25] further showed that TaIce2 binds to the promoter region ...
Epicatechin and cocoa metabolites caused an increase in ApoAI expression in HepG2 cells. Electrophoretic mobility shift assays revealed the involvement of Sites A and B of the ApoAI promoter in the induction of ApoAI mRNA upon incubation with cocoa metabolites. Using supershift assays, we demonstrated the binding of HNF-3β, HNF-4, ER-α, and RXR-α to Site A and the binding of HNF-3β, NFY, and Sp1 to Site B. Luciferase assays performed with a construct containing Site B confirmed its role in the upregulation of ApoAI by cocoa metabolites. Incubation with 3-methyl-epicatechin led to an increase in HNF-3β mRNA, HNF-3β, ER-α, Sp1, and NFY protein levels and the activation of ApoAI transcription mediated by NFY, Sp1, and ER-α. ...
MATERIALS AND METHODS: To this end, first, the MMPs inhibition factor was purified from an alkali-solubilized fraction of RJ (Apis mellifera) by C18 reverse-phase column chromatography and identified as 10-hydroxy-2-decenoic acid (10H2DA) by LTQ XL analysis. Next, examination was made of why 10H2DA could inhibit the activity of MMPs: With RASFs isolated from Rheumatoid tissues by enzymatic digestion, cultures in monolayers were treated with 10H2DA (0. 5, 1, 2mM) or PBS for 2h followed by stimulation with TNF-alpha (10ng/ml) for 2h, mRNA. Protein levels of MMP-1, MMP-3 were measured by real-time PCR and enzyme-linked immunosorbent assay(ELISA), the DNA binding activity of activator protein-1(AP-1) and nuclear factor kappaB (NF-kappaB) by electrophoretic mobility shift assay(EMSA), and the protein kinase activity of p38, ERK and JNK by kinase assay ...
The structure of Cas9 bound to AcrIIA4 suggested that the inhibitor competes for the initial DNA recognition event of PAM binding. However, we previously found that Cas9 binds so tightly to target DNA that its off-rate is negligible (7, 8). This implies an unusual nonequilibrium mode of inhibition, in which AcrIIA4 requires access to Cas9-sgRNA before formation of the Cas9-sgRNA-DNA complex. To test this prediction, we used biolayer interferometry (BLI) to measure the binding of catalytically inactivated Cas9 (dCas9) to a DNA target in the presence of an inhibitor. These experiments were performed under stoichiometric binding conditions in which Cas9 was present at concentrations greater than the dissociation constant of the Cas9-AcrIIA4 interaction. Preincubation of Cas9 with AcrIIA4 markedly inhibited Cas9 binding to DNA (on-rate) in a dose-dependent fashion, including complete prevention of target engagement (Fig. 3C and fig. S7). An electrophoretic mobility shift assay (EMSA) further ...
Methods The antitumor effect of C. crepidioides was evaluated in S-180-cell-bearing mice. Cell growth was assessed using a colorimetric assay. Nitrite and nitrate levels were measured by colorimetry. The expression levels of inducible NO synthase (iNOS) in murine RAW264.7 macrophages was assessed by reverse transcriptase-polymerase chain reaction. Activation of iNOS promoter was detected by reporter gene. Activation of nuclear factor-κB (NF-κB) was evaluated by electrophoretic mobility shift assay. The role of NF-κB signaling was analyzed using inhibitors of NF-κB and dominant-negative mutants, and Western blot analysis ...
Jurkat nuclear extract (1 day growth) for use in WB and EMSA. For studies related to T cells, viral infection (e.g HIV), cytokines, differentiation, and cancer.
In further experiments, EA.hy 926 cells were transiently transfected with different pGl3-Basic-derived constructs containing fragments of 1.6-kb to 0.33-kb of the human eNOS promoter cloned before a luciferase reporter gene. The 1.6-kb-eNOS-promoter-luciferase construct showed a significant (,7.5-fold) increase in (basal) promoter activity compared with pGl3-Basic. The nonstimulated activities of the shorter promoter fragments did not differ significantly from those of p-eNOS-1600-Hu-Luc: p-eNOS-1111-Hu-Luc had 118% ± 21% of p-eNOS-1600-Hu-Luc, p-eNOS-633-Hu-Luc 78% ± 20%, and p-eNOS-326-Hu-Luc 143% ± 37% (n = 3, each). The transfected cells were incubated for 24 h with either ethanol (1.25%, v/v) or the French wine "L.Chevaliers" (10%, v/v). The wine increased the activities of the 1.6-kb-, 1.1-kb-, and the 0.6-kb-promoter fragments more than 2.5-fold; the activity of the 0.3-kb-promoter fragment was still increased 1.7-fold (Fig. 5B).. Using electrophoretic mobility shift assay, we tested ...
Use near-infrared fluorescent oligonucleotides in EMSA applications. Then image on the Odyssey Infrared Imagers for faster, safer gel shift assay imaging.
Buy our human hepatocellular liver carcinoma cell line nuclear lysate, acetaldehyde treated. ab14661 has been validated in electrophoretic mobility shift…
A single-nucleotide polymorphism (C/A) located within an E-box at the −20 position of the human angiotensinogen (AGT) promoter may regulate transcriptional activation through differential recruitment of the transcription factors upstream stimulatory factor (USF) 1 and 2. To study the contribution of USF1 on AGT gene expression, mice carrying a (−20C) human AGT (hAGT) transgene were bred with mice harboring a USF1 gene trap allele designed to knock down USF1 expression. USF1 mRNA was reduced relative to controls in liver (9±1%), perigenital adipose (16±3%), kidney (17±1%), and brain (34±2%) in double-transgenic mice. This decrease was confirmed by electrophoretic mobility shift assay. Chromatin immunoprecipitation analyses revealed a decrease in USF1, with retention of USF2 binding at the hAGT promoter in the liver of male mice. hAGT expression was reduced in the liver and other tissues of female but not male mice. The decrease in endogenous AGT expression was insufficient to alter ...
978 TGFβ is a proliferation suppressor in untransformed epithelial cells. However, in solid tumors, particularly at later stages of disease, increased TGFβ production by the tumor cells contributes to cancer progression through paracrine stimulation of cells in the tumor micro-environment. We therefore proposed that the blockade of TGFβ1 production by the tumor cells would lead to suppression of tumor growth. In this study we investigated the potential components critical for signaling pathways mediating TGFβ1 production using an siRNA approaches, electrophoretic mobility shift assays (EMSA), EMSA super-shift analysis, and TGFβ1 promoter AP-1 luciferase reporter assays. A TGFβ-sensitive, untransformed rat intestinal epithelial cell line, IEC4-1, and a human colon carcinoma (HCC) cell line, FET, were employed in these studies. FET cells stably transfected with tetracycline-controllable dominant-negative mutants of TGFβ type II receptors (DN RII) were also utilized. Our results indicate ...
BACKGROUND. The prostrate cancer (PCa) susceptibility gene glycine N-methyltransferase (GNMT) is associated with the metastasis potential of PCa cells. Androgens, which participate in transcriptional regulation via an androgen receptor (AR), play an important role in PCa development. Our two goals were to determine whether GNMT is regulated by androgen, and to map androgen response elements (AREs).. METHODS. Real-time PCR was used to evaluate GNMT mRNA expression levels after LNCaP and DU145 cells were treated with R1881 (a synthetic AR agonist). Putative AREs were identified and tested using the MatInspector program and a luciferase reporter assay respectively. Electrophoretic mobility shift assay and chromatin immunoprecipitation were used to verify the binding properties of the GNMT-ARE motif.. RESULTS. Results from real-time PCR analyses indicate that R1881 is capable of inducing significant GNMT mRNA levels in AR-positive LNCaP cells, but not in AR-negative DU145 cells. According to ...
The CCD-11Lu human lung fibroblast cell line was used as an in vitro model. Cells were pretreated with CAPE followed by stimulation with interleukin-4 and tumor necrosis factor alpha. The levels of eotaxin in cultured supernatants were measured by enzyme-linked immunosorbent assay. The amounts of STAT6 and phosphorylated STAT6 in cellular nuclear protein extracts were determined by Western blot analysis. STAT6 DNA binding activities were detected by electrophoretic mobility shift assay. ...
All possible two-, three-, and C646 datasheet four-way SNP interactions were tested using 20-fold cross-validation in an exhaustive search (considering all possible SNP combinations). The conditional logistic regression analysis was performed using SPSS (v16.0) to confirm the reported interactive effects in MDR, which may be caused by the main effects from the component loci instead of the epistatic interactions. A logistic regression analysis with P < 0.05 could support the corresponding significant MDR interaction model. Electrophoretic mobility shift assay The human complementary DNA clone of CDX1 (pCMV6-CDX1) was produced by OriGene (OriGene Technologies,. Rockville, MD, USA). CDX1 protein preparation was made by transfecting pCMV6-CDX1 construct into HEK293 cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Cells were harvested 48-h post-transfection, and nuclear extractions were performed. using a nuclear extraction kit (Panomics, Fremont, CA, USA). Protein concentration was ...
As more of the world’s cities become congested and polluted, new business models and technologies are emerging to solve the mobility challenge.
The regulation of macrophage activator protein-1 (AP-1) gene expression by LPS and cytokines is of potentially crucial importance in the pathogenesis of several diseases. The action of LPS and four cytokines on AP-1 gene expression in the murine macrophage J774.2 cell line was, therefore, studied. Exposure of the cells to IL-6 produced no changes in the mRNA levels of all AP-1 members studied. In contrast, the expression of JunB, c-jun and c-fos, but not JunD, was increased by LPS, TNF-α, IFN-γ and IL-1, albeit with different kinetics and magnitude of induction. Electrophoretic mobility shift assays showed a close correlation between the expression of the AP-1 genes and the functional AP-1 DNA binding activity and, additionally, demonstrated the participation of heterodimeric interactions between the different members. These studies provide insights into the potential mechanisms that may be involved in the mediator-specific modulation of AP-1 regulated macrophage gene expression.. ...
Aberrant regulation of the Wnt/β-catenin pathway plays important roles in colorectal carcinogenesis, with over 90% of cases of sporadic colon cancer featuring β-catenin accumulation. While ubiquitination-mediated degradation is widely accepted as a major route for β-catenin protein turnover, little is known about the regulation of β-catenin in transcriptional level. …Elf3, a member of the E-twenty-six family of transcription factors, drives β-catenin transactivation and associates with poor survival of colorectal cancer (CRC) patients. … first found recurrent amplification and upregulation of Elf3 in CRC tissues, and further Gene Set Enrichment Analysis identified significant association between Elf3 expression and activity of WNT/β-catenin pathway. Chromatin immunoprecipitation and electrophoretic mobility shift assay consistently revealed that Elf3 binds to and transactivates β-catenin promoter. Ectopic expression of Elf3 induces accumulation of β-catenin in both nucleus and ...
Tumor suppressor protein p53 possesses two DNA-binding sites. One that is located within its core domain is responsible for sequence-specific DNA binding of the protein, non-specific binding to internal segments of single- or double-stranded DNA, and to certain kinds of non-B DNA structures. The other that is contained in the C-terminus of the protein binds to damaged DNA. Binding of active, latent, and in vitro-activated p53 protein to DNA fragments modified by antitumor cisplatin was studied using electrophoretic mobility shift assay in agarose gels and immunoblotting analysis. We found that both latent and active p53 forms bound to random sequences of DNA globally modified by cisplatin with a higher affinity than to unmodified DNA. Interestingly, the latent form exhibited a more pronounced selectivity for platinated DNA than the active p53. Consistently with this observation, the preference of the latent form for platinated DNA decreased as a consequence of the activation of latent p53 by ...
The project will start with the design and ordering of CooA (wild type/mutants) and its response element (wild type/mutants) sequences. CooA mutants were reported to have higher affinity for CO. They were included in our order to shorten CO response time if needed. Two promoters, PCOOF and PCOOM, were previously reported as strong and weak promoters of CooA. We will also design several PCOOF promoter mutants (point mutations). These mutants are expected to have changed affinity for CooA. Binding affinity of CooA and mutated promoters will be determined as a part of characterization work package which includes Isothermal Titration Calorimetry (ITC), Electrophoretic Mobility Shift Assay (EMSA) and Intrinsic Tryptophan Fluorescence (ITF). Following this, promoters with different CooA affinities will be coupled and constructs will be prepared for in-vivo cell sensor experiments ...
The project will start with the design and ordering of CooA (wild type/mutants) and its response element (wild type/mutants) sequences. CooA mutants were reported to have higher affinity for CO. They were included in our order to shorten CO response time if needed. Two promoters, PCOOF and PCOOM, were previously reported as strong and weak promoters of CooA. We will also design several PCOOF promoter mutants (point mutations). These mutants are expected to have changed affinity for CooA. Binding affinity of CooA and mutated promoters will be determined as a part of characterization work package which includes Isothermal Titration Calorimetry (ITC), Electrophoretic Mobility Shift Assay (EMSA) and Intrinsic Tryptophan Fluorescence (ITF). Following this, promoters with different CooA affinities will be coupled and constructs will be prepared for in-vivo cell sensor experiments ...
Recent reports claimed that thymoquinone exhibited inhibitory results over the cell proliferation of various cancer cell strains. This research was done to investigate the antitumor and anti-angiogenic results of thymoquinone on osteosarcoma in vitro As well as in vivo. Our outcomes confirmed that thymoquinone induced a higher proportion of expansion inhibition and apoptosis within the human osteosarcoma mobile line SaOS-two in comparison with that of Regulate, and thymoquinone considerably blocked human umbilical vein endothelial mobile (HUVEC) tube development inside a dose-dependent way. To analyze the achievable mechanisms linked to these activities, we executed electrophoretic mobility shift assay (EMSA) and western blot Examination, and located that thymoquinone considerably downregulated NF-κB DNA-binding action, XIAP, survivin and VEGF in SaOS-two cells ...
Fewer girls than boys are referred for ADHD treatment, but they have a similar pattern of impairment and receive similar treatment. Evolution of socio-economic inequalities in mortality in small geographical areas of the two largest cities in Spain (Barcelona and Madrid), 1996-2007. An experimental system for the study of metastasis has been developed using an epithelioid cell line of hepatic origin which had previously been chemically transformed in vitro. Electrophoretic mobility shift assays were carried out to study the ability of IntI1 to bind the integrase primary target sites attI and aadA1 attC.. Sutureless repair of uncomplicated gastroschisis can be performed safely, however, it is associated with a significant increase in time to full feeds and time to discharge. Proper preparation for barium study of the stomach should involve treatment with a mucolytic agent about 15 minutes before the examination. There is a renewed interest in teaching psychotherapy in psychiatry training programs ...
Abramson, Harold Alexander, Furchgott, Robert F., Ponder, Eric (March 1939) The electrophoretic mobility of rabbit erythrocytes and ghosts. J Gen Physiol., 22 (4). pp. 545-553. ...