Circulating immune complexes (CIC) were isolated from 25 patients with rheumatoid arthritis (RA) by anti-C1q affinity chromatography. The components were detected by silver stained sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels and identified by the Western blot. The CIC were composed of 20 different polypeptides, including albumin, immunoglobulin, complement, and acute phase reactants. Two components (molecular weight 48 kD and 45 kD respectively) remained unidentified. The 48 kD polypeptide was found in CIC from six out of 14 patients (43%) with extra-articular RA, but from none of eight patients with vasculitic complications of other connective tissue diseases. All immunoreactants were more frequently found in the patients with extra-articular features of RA. Although these results emphasise that most CIC in RA are composed of endogenous proteins, the 48 kD polypeptide is a candidate for an extrinsic antigen in RA. ...
Browse professional translators on TM-Town in the field of Sodium Dodecylsulfate-Polyacrylamide Gel Electrophoresis and language pair of Danish to Hungarian including the top 10 translators by translation units
The objective of this study was to investigate the effect of mungbean protein isolate (MPI) on the potential possibility of water binding agent and as a substrate for the microbial transglutaminase (MTGase) in myofibrillar protein. Cooking loss (CL,%), gel strength (GS, gf), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM) were measured. The addition of MPI reduced CL, indicating that it has a water binding capacity during cooking. The major protein band (53 kDa) of MPI appeared when MPI was mixed with MP, but it disappeared when MTGase was incorporated. MPI treatment changed the endothermic peaks as compared with those of CTL. MTGase (1%) mediated pork MP increased CL and GS (P , 0.05), and reduced peak temperatures with vanishing of endothermic intensity at 1st and 3rd peaks, suggesting the structural changes of protein gelation. In microstructures, MTGase treatment showed a finely stranded ...
A new chitin-binding lectin was purified from a Bangladeshi cultivar Deshi of potato (Solanum tuberosum L.) through anion-exchange and affinity chromatographies using a chitin column. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed the molecular mass of the lectin as 20,000 Daltons. This molecular mass was almost half of the molecular masses of chitin-binding lectins derived from other potatoes. The lectin showed both bactericidal and growth-inhibiting activities against Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli, Salmonella enteritidis and Shigella boydii) pathogenic bacteria. It also showed antifungal activity against Rhizopus spp., Penicillium spp. and Aspergillus niger. Biofilm produced by the bacterium Pseudomonas aeruginosa was dose-dependently reduced by 5-20% in 24 h after administration of the lectin, which was attributed to the glycan-binding property of the lectin having affinity to GlcNAc polymers. It was the first ...
Infection of barley leaves by powdery mildew (Erysiphe graminis f.sp. hordei) causes increased dark respiration, par tof which is associated with active host responses to infection, and a consequence of which is reduced plant growth. The pathogen cannot be grown separately from the host. Therefore, in order to examine those changes in respiratory activity peculiar to the host, attempts were made to isolate protoplasts from infected tissues, and from healthy controls. Isolation of useful numbers (, 106cm−6) of viable mesophyll protoplasts from infected tissues was possible with one among several batches of commercial Cellulysin tested; on analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), this batch contained a low molecular weight protein at 20.1 kDa not found in other batches. In all isolated protoplasts, total respiration increased with the age of the source-leaf, but within 24 h of inoculation respiration was stimulated by infection. Protoplasts from ...
To determine whether there is diversity among clinical isolates of Helicobacter pylori in Chinese patients with peptic ulcer disease, 40 strains of H. pylori were isolated from antral biopsy specimens obtained at the gastroenterology clinic of Xiangya Hospital from January 1996 to June 1998. Total protein profile by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and DNA diversity by polymerase chain reaction-random amplified polymorphic DNA (PCR-RAPD) fingerprinting were performed with these isolates. All the isolates from peptic ulcer disease were relatively homogeneous in protein profiles, but they showed a great DNA sequence diversity by PCR-RAPD fingerprinting. In Chinese patients H. pylori demonstrated an enormous diversity. The diversity among clinical isolates of H. pylori could be distinctly demonstrated and this observation will be helpful in the management of intrafamilial and recurrent H. pylori infection. PCR-RAPD fingerprinting is an efficient method of distinguishing
Background: Prawns and shrimp certainly are a regular cause of sea food allergy mediated by IgE antibodies. adjustments. Quickly the shell and meats of each varieties had been combined in 1 M phosphate-buffered saline (pH 7.2) and extracted overnight in 4 °C under regular blending. The homogenates had been centrifuged at 14 000 rpm at 4 °C for a quarter-hour. The supernatants had been sterile-filtered lyophilised and kept at after that ?20 °C until make use of. For preparation of the boiled extracts the homogenates of the prawns were boiled for 5 minutes before extraction as described above. The protein concentration of each extract was decided using the Total Protein Kit (Sigma-Aldrich UK) and bovine serum albumin as a standard. SDS-PAGE of prawn extracts Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed as described by Nakano et al. (17) with some modifications. The samples of the extracts were heated at 97 °C for 4 minutes and Precision Plus Protein ...
article{9ffe9df9-5a4c-440c-bdd1-534be5ba851a, abstract = {We report a case of a 5-year-old child who suffered an oral allergy syndrome and lip angiedema after eating grapes. We obtained a positive prick test with commercial grape extract and a positive prick-by-prick test with pulp and peel of fresh white grape (Moscatel variety) and pulp and peel of blue grape. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by immunoblotting revealed specific immunoglobulin E (IgE) antibodies in the patients serum against a 94,000 molecular-weight antigenic band. Lip open challenge was positive.}, author = {Rodriguez, Angel and Trujillo, María Jesús and Matheu, Victor and Baeza, María Luisa and Zapatero, Lidia and Martinez, Maribel}, issn = {0905-6157}, language = {eng}, number = {5}, pages = {289--290}, publisher = {Wiley-Blackwell}, series = {Pediatric Allergy and Immunology}, title = {Allergy to grape: a case report}, url = {http://dx.doi.org/10.1034/j.1399-3038.2001.00064.x}, volume ...
Alkaline phosphatase from human liver was purified to homogeneity. The purification procedure included solubilization with butanol, fractionation with acetone, and chromatography on concanavalin A-Sepharose, DEAE-cellulose, Sephadex G-200 and DEAE-Sephadex. Purity was established by standard and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The isoelectric point of the protein was determined to be 4.0. Sephadex-gel filtration gave a mol.wt. of 146000, although a higher value was obtained in the presence of 100mM-NaC1. The subunit mol.wt. 76700, was determined by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. Neuraminidase treatment resulted in two enzyme-activity bands on isoelectric-focused gels with isoelectric points of 6.6 and 6.8. The desialylated enzyme gave only one protein band on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis with a subunit molecular weight indistinguishable from that of the non-neuraminidase-treated protein. The desialylated ...
The giardins are a family of approximately 30000 Mr structural proteins found in microribbons attached to microtubules in the disc cytoskeleton of Giardia. After examining the solubility of giardins in various agents, a method has been developed to extract these polypeptides and subsequently precipitate them selectively. The giardin chains are soluble in 10 mM-HEPES/EDTA buffer at high pH and low ionic strength, but become insoluble in 10 mM-MES/EDTA buffer at pH 6.7 when the ionic strength is raised above 50 mM salt. By dialysing giardin extracts in turn against dissociating and reassembly buffers, the purification is obtained of a subset of giardin chains identified by sodium dodecyl sulphate/polyacrylamide gel electrophoresis as the cytoskeleton bands 14a, 14b and 15. The structures forming under assembly conditions are all composed of fine filaments, 2-3 nm in diameter. Filaments after the first cycle of assembly are found in bundles, narrow ribbons of two or three filaments, and large ...
Both SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) and Western blotting are vital and very common methodology for protein extraction and ...
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TY - JOUR. T1 - An electrophoretic difference between surface and secreted IgM of murine splenocytes. AU - Melcher, U.. AU - Uhr, J. W.. PY - 1973. Y1 - 1973. N2 - μ chains on the surface of murine splenocytes are more heterogeneous on SDS polyacrylamide gel electrophoresis than both secreted and intracellular μ chains labeled with [3H]tyrosine. No difference in heterogeneity among cell surface, secreted, or intracellular L chains was detected. The possible role of carbohydrate in μ chain heterogeneity is discussed.. AB - μ chains on the surface of murine splenocytes are more heterogeneous on SDS polyacrylamide gel electrophoresis than both secreted and intracellular μ chains labeled with [3H]tyrosine. No difference in heterogeneity among cell surface, secreted, or intracellular L chains was detected. The possible role of carbohydrate in μ chain heterogeneity is discussed.. UR - http://www.scopus.com/inward/record.url?scp=0015816392&partnerID=8YFLogxK. UR - ...
misc{eca87be0-f7fa-474e-a1e5-8de2cbfa3eba, abstract = {In order to identify the protein responsible for a dopamine peroxidizing activity, previously described in human normal and parkinsonian substantia nigra by our group, we developed non-denaturing polyacrylamide gel electrophoresis conditions, mimicking the characteristic colour in vitro reaction, resulting from cyclic oxidation of dopamine (DA). After separating protein mixtures from human normal midbrain homogenates on two sets of identical native gels, one gel set was subjected to specific activity staining by using DA and hydrogen peroxide. An activity red/orange band appeared in midbrain tissue lanes, similarly to the lane where commercial horseradish peroxidase (HRP) was present as control of peroxidative activity. The second set of gels, stained with Coomassie Blue, showed other, not enzymatically active protein bands. Mass spectrometry analysis of the bands containing the activity and the corresponding Coomassie Blue bands revealed ...
87 were evaluated for analysis of variability in seed storage proteins by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Electrophorogram for each variety were scored and presence or absence of each band noted and was entered in a binary data matrix. Based on the data of SDS-PAGE gels cluster analysis was performed to check the variations among varieties. The overall result shows low degree of heterogeneity however different varieties reveal differential protein banding pattern. It is concluded that SDS-PAGE analysis of wheat endosperm protein is useful for evaluation of genetic variability and cultivars identification that help in wheat breeding program ...
The present invention relates to a method for detection of protein using a counter-dye composition, on polyacrylamide gels, and the counter-dye composition for detection of protein on polyacrylamide gels. More specifically, the present invention relates to a method for detection of protein in a high sensitivity on polyacrylamide gels in a rapid and simple manner, comprising the step of staining the polyacrylamide gels with a counter-dye composition containing an acidic organic dye and a basic organic dye, and the counter-dye composition for detection of protein on polyacrylamide gels.
G. DUBOIS, J. C. TURPIN, N. BAUMANN; Electrophoretic Characterization of A and B Isoenzymes of Arylsulfatase. Biochem Soc Trans 1 April 1974; 2 (2): 256. doi: https://doi.org/10.1042/bst0020256. Download citation file:. ...
SDS-Polyacrylamide Gel Electrophoresis is a technique to separate proteins according to elctrophoretic mobility - a function of polypeptide chain length or protein mass). SDS-Polyacrylamide Gel Electrophoresis can also be used to separate DNA and RNA molecules.. SDS stands for sodium dodecyl sulfate. SDS is an anionic detergent that disrupts non-covalent interactions in native proteins. SDS is used to create denaturing conditions to separate proteins by molecular weight and also confers negative charge to the proteins in proportion to its mass. By denaturing the proteins with SDS, proteins can be separated by their mass alone; without SDS, other molecular properties, such as a charge and shape, would interfere with the separation process (proteins that are strongly negative, for example, would move faster down a gel, even if they were larger, without SDS). In addition, a loading dye is introduced that helps bind the protein to the gel and make it more recognizable when exposed by UV ...
1. The identification, isolation and characterisation of a mammalian mRNA required choice of a cell type which synthesises only one or a few proteins. The mammalian reticulocyte synthesises globin almost exclusively. This cell type can be isolated in large quantities and has low endogenous ribonuclease activity, thus making it a suitable system for the isolation of large amounts of undegraded RNA. Preliminary experiments indicated that the reticulocyte RNA component sedimenting at 9s had many of the characteristics expected of the globin mRNAs. 2. The characterisation of an mRNA which can only be labelled to a small extent in vivo and which is only about l of the total RNA requires the development of large-scale isolation procedures. Preliminary experiments designed to circumvent the difficulty of labelling reticulocyte 9s RNA in vivo involved the isolation of uridine-9s RNA from cultures of 14 day mouse embryo liver cells. The technique of preparative polyacrylamide gel electrophoresis was ...
The Novex® NuPAGE® SDS-PAGE Gel System is a revolutionary high-performance polyacrylamide gel electrophoresis system that simulates the denaturing conditions of the traditional Laemmli system (Tris-glycine SDS-PAGE gels) without using SDS detergent. NuPAGE® SDS-PAGE Gels use a unique buffer formulation that maintains a low operating pH during electrophoresis. This eliminates the
Analysis of Mutant SOD1 Electrophoretic Mobility by Blue Native Gel Electrophoresis; Evidence for Soluble Multimeric Assemblies. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
TY - JOUR. T1 - Fast staining and destaining of sodium dodecyl sulfate-polyacrylamide gels. AU - Lloyd, Matthew D.. PY - 1996/10/1. Y1 - 1996/10/1. UR - http://linkinghub.elsevier.com/retrieve/pii/S0003269796903899. UR - http://dx.doi.org/10.1006/abio.1996.0389. U2 - 10.1006/abio.1996.0389. DO - 10.1006/abio.1996.0389. M3 - Article. VL - 241. SP - 139. EP - 140. JO - Analytical Biochemistry. JF - Analytical Biochemistry. SN - 0003-2697. IS - 1. ER - ...
This new edition is almost a completely new text, with eight of the ten chapters written by new authors. It presents the most reliable methods for essential procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements of the procedures. In addition, it describes major new techniques developed since the previous edition.
I am planning to run a Blue Native gel, followed by electroblotting for Western analysis. I have read that the Coomassie blue that binds proteins in Blue Native electrophoresis interferes with protein transfer to a membrane. Yet, there are many reports of transfer from BN gels. Does anyone have experience with this procedure who could give me some tips? And is it possible that a nitrocellulose membrane presents more of a problem than PVDF? Thanks for any help. Nora Plesofsky ...
Table E1 in the online supplement), as classified previously (25). ELISA kit (Roche Applied Technology, Burgess Hill, UK) according to the manufacturers instructions. A more detailed description is supplied in the web dietary supplement. Real-time Polymerase String Response Total RNA was isolated using RNeasy Mini Package (Qiagen, Western Sussex, UK) and reverse-transcribed with random primers and AMV reverse transcriptase (Promega, Southampton, UK). mRNA was quantified by real-time polymerase chain reaction (PCR) (Rotor Gene 3000; Corbett Study, Sydney, Australia) using SYBR Green PCR Expert Blend Reagent Mouse monoclonal to PTEN (Qiagen) and QuantiTect primer assays (Qiagen) for HO-1, NQO1, MnSOD, catalase, and IL-6. mRNA manifestation was normalized to 18S rRNA manifestation. A more detailed description is offered in the online supplement. Western Blotting Whole-cell protein extracts were fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to a ...
Novex Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both Morenative and denatured proteins. Novex Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands ...
SDS-PAGE analysis of recombinant NCAM and FGFR1 proteins. Coomassie blue-stained gel of His-tagged soluble proteins expressed in 293-EBNA cells. The positions o
Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis, or SDS-PAGE, is a widely-used technique for separating mixtures of proteins based on their size...
To study the mechanisms of RNA splicing we have analyzed the products generated by in vitro processing of a truncated 32P-labeled human beta-globin RNA precursor that contains the first two exons and the first intervening sequence (IVS1). Six major RNA products were detected and characterized. The first detectable RNA processing event is cleavage at the 5 GT of IVS1. Subsequently, accurately spliced RNA and the excised, intact IVS1 are simultaneously observed. The IVS1-containing RNA processing products have several unusual properties, which include: anomalous electrophoretic mobilities on polyacrylamide gels; a block to reverse transcription near the 3 end of IVS1; the presence of a nuclease-resistant component within IVS1. The block to reverse transcription and the nuclease-resistant component map to the same site near the 3 end of IVS1. The nuclease-resistant component appears to be a modified adenosine residue that contains an RNA branch. Based upon these and other structural studies we ...
A computer-implemented method, computer program product, and system for detecting anomalous behavior of computing devices are provided. The computer-implemented method for detecting anomalous behavior of computing devices may include establishing a network of computing devices; receiving shared data from the networked devices; determining device behavior; predicting future device behavior, detecting anomalous device behavior, and sending an alert in response to a detection of anomalous device behavior.
By way of example, females endure a higher extent of endothelial and smooth muscle dysfunc tion in contrast with men. Hence, it has been suggested the endothel
Does anyone have any hints for transferring proteins from non- denaturing poly-acrylamide gels to nitro-cellulose? I have been transferring multi-protein/DNA complexes from EMSA gels with varying success, and I am wondering whether or not a step such as soaking the shift gel in SDS prior to transfer would aid the transfer. ANy other hints. Thank you. Dave ...
Shop online for a wide selection of Alfa Aesar Laemmli SDS Sample Buffer, reducing, 6X For protein sample preparation to be used in the Laemmli SDS-PAGE system
Id like to know if somebody has found the same problem when running big proteins (about 100KDa) in PAGE. I use a really specific antibody to develop western blot and Ive detected that: when I use an 8%-acrilamide gel, this protein appears with his specific size, 100KDa; BUT when I use a 10%-acrilamide gel, my before-specific-antibody now detects a really intensiv band, but very much faster (lower size) than before!! I cant understand this. Ive just studied a lot of conditions, like frozen samples, lesser boiling time, changing lysis buffer, ect ...
A biomarker, or biological marker, is in general a substance used as an indicator of a biological state. It is a characteristic that is objectively measured and evaluated as an indicator of normal biological processes, pathogenic processes, or pharmacologic responses to a therapeutic intervention. Biomarker discovery has been accelerated by proteomics, which study to entire protein expression at any given time in cell or tissue. If a certain protein is the functional molecule in cells, measurement of the differential expression of proteins could indicate disease-specific changes in tissues or organs.
Identity tests rely on the genetic differences among individuals. The most informative genetic markers for the genetic characterization of people are variable number of tandem repeat (VNTR) loci...
Gel electrophoresis is a technique that involves the movement of molecules through a gelatin-like material in an electrical field. Since the distance that a molecule will move is dependent on its size, gel electrophoresis is a good technique to separate different size DNA fragments. The smaller fragments will move the farthest from the sample well. An electrophoresis apparatus has five major components: (a) electrical current; (b) the test sample (e.g., DNA); (c) the gelatin medium (agarose) that the sample moves through; (d) a liquid to conduct the electrical current (usually a buffer); and (e) a stain used to highlight the migrated samples ...
Referenced from the main protocol, any comments about the protocol should be made here; i.e. how it was developed. Any comments added should be signed (by adding *~~~~: in front) and explained. Links to FAQs/tips provided by other sources such as the manufacturer or other websites would be best made here ...
十二烷基硫酸钠聚丙烯酰胺凝胶电泳,也称SDS-PAGE,是一种被广泛使用,仅根据分子量大小来分离蛋白质混合物的技术。 阴离子去污剂SDS,在变性的线性蛋白表面沿长度均匀分布使其带电。 将它们上样到聚丙烯酰胺凝胶后,...
コンテンツ --, ,div id=Content, ,h2,AFM,/h2, ,img src=http://openwetware.org/images/7/76/SANY0194.JPG alt=AFM width=580 height=400/, ,p, ,img src=http://openwetware.org/images/1/1b/SANY0195.JPGwidth=200 height=180/,   AFM enables us to observe nano size structures composed of DNA. Make an observation sample. We put an observation object on a mica for five minutes and wash it in buffer. (Tris/Tris-HCL 20 mM Mg,sup,2+,/sup, 12.5 mM pH = 7.4) ,/p, ,h2, Electrophoresis,/h2, ,p,,img src=http://openwetware.org/images/8/88/SANY0197.JPG alt=AFM width=580 height=400/,   We can see whether constructing DNA structures is achieved or not by Electrophoresis. It also informs us of DNA length of approximately.,/br,   The electrophoretic condition is cf. Result & Method.,/font,,/p, ,h2, How to create micelle,/h2, ,p,,img src=http://openwetware.org/images/1/17/%E8%B6%85%E9%9F%B3%E6%B3%A2%E3%83%9B%E3%83%A2%E3%82%B8%E3%83%8A%E3%82%A4%E3%82%B6%E3%83%BC.jpg alt=soni ...
Sodium, Electrophoresis, Escherichia, Escherichia Coli, Family, Flexibility, Inhibition, Muscle, Mutagenesis, PH, Polyacrylamide Gel Electrophoresis, Polymerization, Polymers, Report, Serpins, Site-directed Mutagenesis, Skeletal Muscle, Sodium Dodecyl Sulfate, Temperature
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Proteins are separated based on their size and charge with gel electrophoresis. Polyacrylamide gel is typically used and with the presence of an electrical field, the proteins molecules are mobilize
Since the New Bands Panel was launched early this year, weve reviewed more than 80 bands and counting. The vast majority are unsigned, but weve also seen a number of our alums begin to enjoy some greatly deserved success as the months have gone on.. To celebrate some of the great talents weve discovered along the way, we asked our panellists to vote for their favourite acts to date.. We then got those bands to agree to offer up one of their tracks as a free download for the month of December, so today we can present to you our end-of-year playlist, The Best of the FFS New Bands Panel 2010. The downloads will be available either until our download limit has run out or until December 31st comes - whichever is first - so get cracking. You can download each track individually, or skip to the bottom to get them all in one. The voting took place a few weeks ago now, so no doubt some of the acts weve come across since will have to make do with a spot on the 2011 list, but for now, take a listen. ...
Electrophoresis is a technique of isolating and visually identifying different biomolecules. This is done by passing an electrical current through the gel to separate charged molecules of different weights. If the molecule youre interested in isnt common enough, its band will be virtually invisible and difficult to measure.. DNA and RNA can be amplified somewhat before running electrophoresis, but it isnt practical to do this with proteins. Therefore, a large tissue sample is needed to run these assays. This can limit the usefulness of the technique, especially in medical analysis. Its virtually impossible to run electrophoresis on samples from a single cell; flow cytometry and immunohistochemistry are more commonly used to assess cell-by-cell expression of proteins. A technique called PCR is excellent at precisely measuring tiny amounts of RNA.. ...
Christian Hogdohm Gel Electrophoresis I. Introduction: A typical electrophoresis has five major parts: the electrical current, DNA, RNA, or protein
Gel Electrophoresis, Agarose, Agarose additive, UV transparent sheets, permeable support mesh, drying frames, rapid coomassie stain, gel box, etc - Page H10
The (Chilean) flat oyster, Ostrea chilensis, is native to New Zealand and the west coast of South America. It is a commercially important species in New Zealand because of its exquisite taste that attracts premium prices. This thesis describes the first isolation and partial charcterisation of an oyster haemolymph calcium-dependent carbohydrate-binding protein. This protein chiletin was originally isolated from oyster haemolymph by binding to the agarose-galactan matrix of a Sepharose column. Chiletin was predominantly composed of a 24 kilodalton (kDa) band when examined with one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis under non-reducing conditions and a 12 kDa band with reduction of disulphide bonds. The N-terminal sequence of the 24 kDa band was determined to be IAGPGWEKYN. This sequence was not homologous to any known protein. Examination of isolated chiletin with two-dimensional protein analysis gel electrophoresis revealed the presence of three (~12 kDa) ...
About 10% of the Chinese population are chronic carriers of hepatitis B virus (HBV). Thus, the development of a highly efficient process for the preparation of a vaccine based on a recombinant hepatitis B surface antigen (HBsAg) is very important to the Chinese national immunization program. To this end, the ion exchange chromatography recovery of CHO-HBsAg from a recombinant Chinese hamster ovary cell line was shown to increase from about 55 to 80% by the addition of 1% poly(ethylene glycol) (PEG 10,000) to the mobile phase. Furthermore, based on analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the intact glycoprotein form of CHO-HBsAg was completely preserved by the addition of PEG. In the absence of PEG the glycoprotein form of CHO-HBsAg was also spread out into the high salt elution fraction. High-performance size-exclusion chromatography with on-line multiangle-laser-light scattering (HPSEC-MALLS) analysis was performed to monitor the status of the ...
SUMMARY: Fourteen strains of Streptococcus mutans serotype c were examined for their cell-surface protein antigens in terms of hydrophobicity, M r and immunochemical specificities. Thirteen strains were hydrophobic, while strain GS-5 was markedly hydrophilic as compared to the other strains tested. Cell-surface protein antigens were then analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western immunoblotting. A protein antigen of M r 190000 (PAc) was found in cell extracts and culture supernatants of all the hydrophobic strains. Neither culture supernatant nor cell extract of strain GS-5 contained PAc. Strain GS-5, however, produced extracellularly a large amount of a protein of M r 155000 (PAGS-5) which reacted with rabbit anti-PAc serum. Immunodiffusion analysis showed that PAGS-5 lacked a part of the antigenic moieties in the PAc molecule. SDS-PAGE and radioimmunoassay showed a small amount of PAGS-5 on the cell surface of strain GS-5. These findings suggest that
The effects of anoxic submergence (16 h at 15°C) on cellular mRNA contents were assessed in five organs of anoxia tolerant turtles Trachemys scripta elegans. Poly(A)+ RNA was extracted from liver, red and white skeletal muscle, kidney and heart of control and anoxic turtles, as well as from heart and kidney of turtles allowed 24 h aerobic recovery (at 15°C) after anoxia exposure. Poly(A)+ RNA content increased by 30% in white muscle from anoxic turtles relative to control animals but was unchanged by metabolic state in other organs. Extracted mRNA was translated in vitro in a wheat germ lysate system and the 35S-labelled polypeptides that were produced were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Overall translational activity of the mRNA pool [cpm 35S-methionine incorporated per microgram poly(A)+ RNA] was altered by anoxia exposure in three organs, increasing by 38 and 18% in liver and kidney and decreasing by 42% in red muscle. Anoxia exposure also led to ...
The isolation of a salt-soluble homogeneous elastin from the aortas of lathyritic chicks by chromatography on DEAE-cellulose and salt precipitation is described. These new techniques, as well as some previously published by other workers, were evaluated with the help of antiserum raised in sheep against insoluble chick elastin. The purified elastin was very basic and behaved in a predictable manner in coacervation studies. The protein migrated in sodium dodecyl sulphate-polyacrylamide gels as a single band moving slightly faster than pyruvate kinase (mol.wt. 57000).. ...
TY - JOUR. T1 - Separation of rat pituitary growth hormone and prolactin by SDS polyacrylamide gel electrophoresis. AU - Zanini, A.. AU - Giannattasio, G.. AU - Meldolesi, J.. PY - 1974. Y1 - 1974. N2 - Isolation of growth hormone (GH) and prolactin (PRL) from rat pituitary homogenates and cell fractions was carried out by polyacrylamide gel electrophoresis using a high resolution Na dodecylsulfate (SDS) system. With this technique GH and PRL are resolved as discrete and well separated bands. The method appears particularly adequate for quantitative determinations of the hormones and of the hormone bound radioactivity not only in pituitary homogenates but also in isolated cell fractions, since the treatment with SDS results in the complete solubilization of all samples and therefore allows the electrophoretic migrations of all hormone molecules. Using the SDS gel system, it was also possible to estimate the degree of contamination by nonhormone proteins in GH and PRL bands separated by the usual ...
A novel immunogenic antigen, the 6-kDa early secretory antigenic target (ESAT-6), from short-term culture filtrates of Mycobacterium tuberculosis was purified by hydrophobic interaction chromatography and anion-exchange chromatography by use of fast protein liquid chromatography. The antigen focused at two different pIs of 4.0 and 4.5 during isoelectric focusing, and each of these components separated into three spots ranging from 4 to 6 kDa during two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The apparent differences in molecular masses or pIs of these isoforms were not due to posttranslational glycosylation. The molecular weight of the purified native protein was determined by applying gel filtration and nondenaturing polyacrylamide gel electrophoresis and found to be 24 kDa. ESAT-6 is recognized by the murine monoclonal antibody HYB 76-8, which was used to screen a recombinant lambda gt11 M. tuberculosis DNA library. A phage expressing a gene product recognized by ...
Protein electrophoresis is a method for analysing the proteins in a fluid or an extract. The electrophoresis may be performed with a small volume of sample in a number of alternative ways with or without a supporting medium: SDS polyacrylamide gel electrophoresis (in short: gel electrophoresis, PAGE, or SDS-electrophoresis), free-flow electrophoresis, electrofocusing, isotachophoresis, affinity electrophoresis, immunoelectrophoresis, counterelectrophoresis, and capillary electrophoresis. Each method has many variations with individual advantages and limitations. Gel electrophoresis is often performed in combination with electroblotting immunoblotting to give additional information about a specific protein. Because of practical limitations, protein electrophoresis is generally not suited as a preparative method. SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a collection of related techniques to separate proteins according to their electrophoretic mobility (a ...
Escherichia coli IMP dehydrogenase (EC 1.2.1.14) was purified by affinity chromatography on immobilized nucleotides. The enzyme binds to agarose-bound 8-(6-aminohexyl)-AMP, N6-(6-aminohexyl)-AMP and 8-(8-amino-octyl)-IMP but not to immobilized NAD+ or Cibacron Blue F3G-A. AMP proved to be an effective eluent. A large-scale purification scheme in which 8-(6-aminohexyl)-AMP-agarose was used resulted in a homogeneous preparation of IMP dehydrogenase. The enzyme was also purified by immunoprecipitation with monospecific antisera. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, N-terminal amino acid analysis and tryptic finger-printing demonstrated that IMP dehydrogenase comprises identical subunits of mol.wt. 58000. Trypsin and Pronase cleave the 58000-mol.wt. subunit into peptides of mol.wts. 42000 and 14000, with a concomitant decrease in enzyme activity. These observations rationalize much of the contradictory data on the subunit composition of the enzyme found in the literature. ...
Methods for microbial classification are not always capable of distinguishing between isolates at the species level. We have previously characterised four Ferroplasma isolates that were ,98.9% similar at the 16S rDNA level, the isolates showed marked phenotypic differences, and one isolate was borderline on the 70% species boundary from DNA-DNA similarity data. In this study we have used statistical comparisons of two-dimensional polyacylamide gel electrophoresis gels for classification of closely related isolates. From the protein profile similarities an un-rooted tree was constructed that was congruent with a tree derived from DNA-DNA similarities.. ...
Any type of data that can be translated into a densitometric curve is considered a fingerprint type in the BioNumerics and GelCompar II software. This includes commonly used genotyping methods employing agarose or polyacrylamide slab gel electrophoresis (PFGE, rep-PCR, RAPD, PCR-DGGE, etc.), in which case the data are usually imported as two-dimensional gel images (bitmaps). Another major group consists of capillary electrophoresis profiles such as AFLP, ARISA, T-RFLP, etc. Here, the raw electropherograms generated by an automated sequencer (genetic analyzer) or derived peak table text files can be imported. Finally, any other profile (generated e.g. by gas chromatography, HPLC or spectrophotometry) that can be seen as peaks or bands, can be analyzed as a fingerprint.
Any type of data that can be translated into a densitometric curve is considered a fingerprint type in the BioNumerics and GelCompar II software. This includes commonly used genotyping methods employing agarose or polyacrylamide slab gel electrophoresis (PFGE, rep-PCR, RAPD, PCR-DGGE, etc.), in which case the data are usually imported as two-dimensional gel images (bitmaps). Another major group consists of capillary electrophoresis profiles such as AFLP, ARISA, T-RFLP, etc. Here, the raw electropherograms generated by an automated sequencer (genetic analyzer) or derived peak table text files can be imported. Finally, any other profile (generated e.g. by gas chromatography, HPLC or spectrophotometry) that can be seen as peaks or bands, can be analyzed as a fingerprint.
An apparent receptor for the egg peptide speract (Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly) was identified by covalently coupling a radiolabeled speract analogue to intact spermatozoa and was then purified by DEAE-Sepharose chromatography and preparative gel electrophoresis after solubilization with Lubrol PX. The purified, crosslinked protein was digested with Staphylococcus aureus V8 protease and a resultant peptide, purified from polyacrylamide slab gel slices, was shown to have the amino acid sequence Val-Ser-Ala-Pro-Phe-Asp-Leu-Glu-Ala-Pro-Phe-Ile-Ile-Asp-Gly-Ile. Polyclonal antiserum, generated against a synthetic peptide that corresponded to the above sequence, immunoprecipitated the radiolabeled crosslinked protein and reacted with a Mr 77,000 protein on immunoblots, demonstrating that the sequenced peptide originated from the apparent receptor. A clone containing a 2.5-kilobase insert was subsequently isolated from a sea urchin testis cDNA library that contained DNA sequences encoding an ...
An apparent receptor for the egg peptide speract (Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly) was identified by covalently coupling a radiolabeled speract analogue to intact spermatozoa and was then purified by DEAE-Sepharose chromatography and preparative gel electrophoresis after solubilization with Lubrol PX. The purified, crosslinked protein was digested with Staphylococcus aureus V8 protease and a resultant peptide, purified from polyacrylamide slab gel slices, was shown to have the amino acid sequence Val-Ser-Ala-Pro-Phe-Asp-Leu-Glu-Ala-Pro-Phe-Ile-Ile-Asp-Gly-Ile. Polyclonal antiserum, generated against a synthetic peptide that corresponded to the above sequence, immunoprecipitated the radiolabeled crosslinked protein and reacted with a Mr 77,000 protein on immunoblots, demonstrating that the sequenced peptide originated from the apparent receptor. A clone containing a 2.5-kilobase insert was subsequently isolated from a sea urchin testis cDNA library that contained DNA sequences encoding an ...
The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) protocol involving 22% (w/w) polyethylene glycol (PEG)-10,000, and 18% (w/w) citrate with a yield of 39.7%, specific activity of 2600 U/mg and purification factor of 4.8. It was shown to have a molecular weight of 40 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The purified thermophile enzyme was stable in alkaline pH range (9.0-11.0) with the optimum pH of 9.0. It was highly stable at 60 °C and retained 100% activity even after 90 minutes, suggesting that it belong to the family of thermophilous. Collectively, our obtained data revealed that the thermophilic protease produced by B.
TY - JOUR. T1 - Electrochemical and homogeneous exchange kinetics for transition-metal aquo couples. T2 - Anomalous behavior of hexaaquoiron(III/II). AU - Hupp, Joseph T. AU - Weaver, Michael J.. PY - 1983. Y1 - 1983. N2 - Rate data for electrochemical and homogeneous redox reactions involving Ruaq 3+/2+, Vaq 3+/2+, Feaq 3+/2+, Euaq 3+/2+, and Craq 3+/2+ redox couples (where aq represents aquo ligands) have been analyzed and compared by using the rate relations due to Marcus in order to ascertain how the kinetics of outer-sphere electron exchange are dependent upon the metal redox center. The work-corrected rate constants for electrochemical exchange at mercury electrodes, ke ex, were found to be in uniformly good agreement with the rate constants for homogeneous self-exchange, kh ex, extracted from cross-reaction data involving outer-sphere coreactants, yielding the reactivity sequence Ruaq 3+/2+ , Vaq 3+/2+ , Feaq 3+/2+ ≳ Euaq 3+/2+ ≳ Craq 3+/2+. However, the measured rate constant for ...
Two-dimensional gel electrophoresis of nuclear phosphoproteins of Novikoff hepatoma and regenerating liver.: Two-dimensional polyacrylamide gel electrophoresis
Blood platelets are important components of hemostasis, contributing to healing of wounds by forming thrombi and to the initiation of repair processes. They are also involved, however, in the...
Breast cancer is one of the most common cancers in women, and rated the second most common cancer and a significant cause of death in females in the Sudan. This study aims to identify tumor protein that elicits humoral immune responses in breast cancer patient in comparison to tissues from healthy individuals as well as from normal tissues of the cancer patient. Serum samples and breast cancer tissue specimens were collected from breast cancer patients (n = 9) and from healthy individuals (n = 5) at Khartoum Teaching Hospital. Breast cancer tissues were homogenized in PBS, centrifuged and the supernatants were lysed in 2X SDS-PAGE sample buffer. The preparation then boiled and the resulting supernatants were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Total proteins were separated on SDS-PAGE and transferred to the nitrocellulose paper, then analyzed by immunoblotting for total proteins and serum antibodies using serum from patients
The [35S]methionine-labeled proteins released in the medium conditioned by normal and transformed mouse fibroblasts have been analyzed by sodium dodecyl sulfate-polyacrylamide electrophoresis. Three major proteins, fibronectin, procollagens, and a protein with a molecular weight of 45,000 (45K protein) have been identified. The 45K protein, which has not yet been described, accounts for about 30% of the proteins released by control 3T3 fibroblasts or mouse embryo cultures. Quantitation of the radioactivity incorporated by the 45K protein indicated a 10- to 15-fold decrease in 3T3 fibroblasts transformed by Kirsten, Abelson, or Rous sarcoma viruses. The amounts of fibronectin and procollagens released in the medium by transformed cells were also reduced by factors of 3- and 5-fold, respectively. Pulse chase experiments have shown that the decreased level of the 45K protein in the medium of transformed cells cannot be explained by a reduced rate of secretion or by extracellular proteolytic ...
Mouse lymphocyte H-2 and Ia glycoproteins have been analyzed with a two-dimensional (2-D) acrylamide gel electrophoresis technique, in which proteins are separated first according to their charge in isoelectrofocusing gels and then according to their size in sodium dodecyl sulfate gels. Individual polypeptide chains from radiolabeled cells are resolved as discrete spots on autoradiograms of the gels, forming patterns which are characteristic of the proteins in the sample. 2-D gels of H-2K, H-2D, and Ia glycoproteins immunoprecipitated from 35S-methionine-labeled cells reveal that these proteins exist in the cells as complex arrays of molecules heterogeneous in both size and charge. Lactoperoxidase-catalyzed radioiodination of lymphocyte surfaces labels only subsets of the total H-2 and Ia molecules with 125I, indicating that some of the molecules may represent cytoplasmic precursors of the cell surface proteins. This theory is supported by the kinetics of labeling of various spots in ...
The [35S]methionine-labeled proteins released in the medium conditioned by normal and transformed mouse fibroblasts have been analyzed by sodium dodecyl sulfate-polyacrylamide electrophoresis. Three major proteins, fibronectin, procollagens, and a protein with a molecular weight of 45,000 (45K protein) have been identified. The 45K protein, which has not yet been described, accounts for about 30% of the proteins released by control 3T3 fibroblasts or mouse embryo cultures. Quantitation of the radioactivity incorporated by the 45K protein indicated a 10- to 15-fold decrease in 3T3 fibroblasts transformed by Kirsten, Abelson, or Rous sarcoma viruses. The amounts of fibronectin and procollagens released in the medium by transformed cells were also reduced by factors of 3- and 5-fold, respectively. Pulse chase experiments have shown that the decreased level of the 45K protein in the medium of transformed cells cannot be explained by a reduced rate of secretion or by extracellular proteolytic ...
Tankan, Rajani, Vasant Patil, Prasanna Aithal (2014) Clinical study on different procedures of nasya with bhringaraja taila in khalitya (alopecia). [Publication] Full text not available from this repository ...
A subset of patients with hypersensitivity reactions to the aromatic anticonvulsants phenytoin, carbamazepine, and phenobarbital have circulating antibodies that recognize members of the rat cytochrome P450 (CYP) 3A subfamily. These antibodies do not recognize related human CYP3A proteins despite the high degree of structural similarity. To investigate the relationship between P450-mediated drug metabolism and the development of anti-P450 antibodies, we initiated epitope mapping studies by screening a library of fusion proteins constructed from rat CYP3A1 with an anti-CYP3A1-positive patient serum sample. Positive signals from colony lifts were confirmed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and immunoblotting, and a 26-amino acid sequence corresponding to amino acids 342-367 of the CYP3A1 protein (NKAPPTY-DTVMEMEYLDMVLNETLRL) was identified as containing the epitope recognized by IgG3 antibodies in this serum sample. By subjecting inserts from two clones into a second ...
A cytosolic insulin-sensitive serine kinase has been purified to apparent homogeneity in parallel from livers of control or acutely insulin-treated rats. The kinase is labile and requires rapid purification for stability. The kinase migrates as a band of apparent Mr = 90,000 on denaturing gels and elutes as a monomer on Superose 12 gel filtration. After sodium dodecyl sulfate-polyacrylamide gel electrophoresis and renaturation, the 90-kDa band presumed to be the kinase shows kinase activity toward myelin basic protein in situ. Substrates of the kinase include Leu-Arg-Arg-Ala-Ser-Leu-Gly (Kemptide), ribosomal protein S6, S6 peptide, a proline-rich peptide substrate, microtubule-associated protein 2, and myelin basic protein. The kinase also phosphorylates histones H1 and H2B, but does not autophosphorylate to a significant stoichiometry. The activity of the kinase is inhibited by fluoride, glycerophosphate, p-nitrophenyl phosphate, p-nitrophenol, heparin, quercetin, poly-L-lysine, and potassium phosphate
Photographs of two-dimensional electrophoresis gels with annotation of the spots of identified proteins. The left image shows a silver-stained gel of embryo chi
A curved-surface cassette/gel system is disclosed in which the walls of a cassette and the major surfaces of a slab-shaped electrophoresis gel in the cassette coact to substantially exclude liquid or gas from between either wall and the major surfaces of the gel. Exclusion is accomplished by exerting a normal force at all points on the walls of the cassette and at all points on the major surfaces of the gel. A curvature may be present in at least one wall of the cassette and in at least one major surface of the cassette. A cassette headpiece may be divided by septa which form an edge seal with the slab gel. The spaces formed between the septa function as wells into which sample materials may be placed.
MODEL RELEASED. Protein analysis. Technician using a proteomics imaging system to analyse proteins isolated by SDS polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE is used to analyse the protein composition of cells and tissues. A sample has SDS (sodium dodecyl sulphate) added to it, which denatures it and applies an overall negative charge. It is then placed into a gel that has an electrical current passed through it, which separates the samples proteins based on their differences in size and charge. Photographed at Dundee University in Dundee, Scotland. - Stock Image C001/8753
ice for 30 min. The concentration of the extracted protein was quantified by the Bradford protein assay kit. Later, the protein samples were mixed with 2× protein buffer and boiled for 5 min. The lysates of treated and control cell samples were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrotransfered onto a polyvinylidene difluoride membrane. The membranes were blocked with 5% bovine serum albumin in Tris-buffered saline-Tween-20 (TBST) and then incubated with the primary Pertussis Toxin at 4 °C overnight. Subsequently, the membranes that were washed three times with TBST for about 15 min each time were further incubated with the corresponding secondary antibodies for 1.5 h. The protein bands were examined using the ECL reagent according to instructions and visualized by the Quantity One software. 2.10. Statistical analysis ...
Medox-Bio electrophoretic systems are designed to suit various applications related to Nucleic acid separation, Protein seperation, Immuno protein separation and Protein transfer in laboratories. These computer designed systems are made out of well-polished and clear Plexiglass sheets fused together by using high-strength specific adhesives.
Fingerprint Dive into the research topics of Immunochemical characterization of human TL-like (T48) and Ly 1-like (T72) glycoproteins using two-dimensional polyacrylamide gel electrophoresis. Together they form a unique fingerprint. ...
A first step in characterizing a protein often involves determining its molecular weight. From this information, different proteins may be compared and the number of amino acid residues in a protein can be determined. Here, students determine the molecular weight of two unknown proteins by comparing their electrophoretic migration with the migration of standard proteins. The protein standards and unknowns have been pre-stained so that your students can follow ...
Gel electrophoresis is the most commonly used technique to study DNA. DNA is a very large molecule that contains genetic information. DNA can be broken down to smaller pieces of different sizes and these pieces are then separated using gel electrophoresis. DNA always has a negative charge, and moves towards the anode. Proteins are large and complex molecules made of amino acids. Proteins can be studied by gel electrophoresis in two ways. One way is to take a mixture of proteins and separate them in the gel. The other way is to break down a single protein into smaller pieces. The smaller pieces can then be separated in the gel. Proteins can be positively or negatively charged. To separate proteins by size only, protein mixtures can be coated with a chemical called sodium dodecyl sulfate (SDS) to give all proteins a negative charge before putting the mixture into the gel. ...
Nurkhamidah, S. and Woo, E. M. (2011), Effects of crystallinity and molecular weight on crack behavior in crystalline poly(L-lactic acid). J. Appl. Polym. Sci., 122: 1976-1985. doi: 10.1002/app.34021 ...
Polyacrylamide gel electrophoresis is a widely used method to study short DNA fragments in solution. It is, however, a relative method requiring length markers to assess mobility, shape, flexibility, and molecularity of the DNA structures of interest. In recent literature we have encountered the use of oligo(dT) fragments as the native PAGE length markers. We show here that this practice is inadequate because oligo(dT) migration is strongly retarded in native polyacrylamide gels. This conclusion is qualitatively true irrespective of the conditions of electrophoresis, oligo(dT) length, and gel concentration. Depending on their length, oligo(dT) fragments migrate 2-4 times slower than that would correspond to their nucleotide number. This leads to erroneous conclusions, e.g., determination of the number of associated molecules in guanine quadruplexes or other DNA complexes. (c) 2006 Elsevier Inc. All rights reserved ...
Dissociative effect of Nutlin-3a, Nutlin-3b, and RO-5963 on the preformed p53·MDM2 complex. Representative electrophoresis polyacrylamide gels in nondenaturing conditions for the analysis of the dissociation power of the inhibitors tested. (A) The reaction mixture containing 25 M p53·MDM2 complex, treated in the absence (lane 3) or in the presence (lanes 4-14) of 0.040, 0.060, 0.080, 0.12, 0.33, 0.67, 1.33, 3.33, 6.67, 33.33, and 333.33 μM Nutlin-3a, respectively, were run on a 12% nondenaturing polyacrylamide gel. Thirty micromolar MDM2 and p53 was run alone in lanes 1 and 2, respectively. (B) As in (A), but using Nutlin-3b, at concentration of (lanes 4-14) of 1.33, 6.67, 13.33, 20.00, 66.67, 133.33, 266.67, 333.33, 400.00, 833.33, and 1666.67 μM. p53 and MDM2 (25 μM both) were run alone in lanes 1 and 2, respectively. (C) As in (A), but using RO-5963, at concentration of 0.10, 0.21, 0.42, 0.83, 1.67, 3.33, 8.33, 16.67, 33.33, 166.67, and 333.33 μM (lanes 4-14), respectively. (D) The ...
Browse Sigma-Aldrichs SDS-PAGE to find products in Acrylamides, Buffers, Detergents, Gel Casting Reagents, Gel Cross-Linkers, Gel Loading Reagents, Ready-to Use Gels, Reducing Agents, TruPAGE™ Precast Gels, Urea
TAE Buffer 50X conc.Wielkość opakowania: 500 mlTAE Electrophoresis Buffer (50X) is a solution used in Agarose Gel Electrophoresis (AGE) typically for the separation of nucleic acids (i.e. DNA and RNA) and as a running buffer for preparative work. Tris-Acetate-EDTA (TAE) is not only used in nucleic acid agarose and polyacrylamide gel electrophoresis but also in agarose and polyacrylamide gel preparation ...
Results. The levels of BD were most influenced by methods 2 and 3, and remained almost unchanged when the method 1 was used. The lag-time of 99mTc-LDL produced by method 2 doubled but it was decreased by 23% when the method 3 was employed. No change in the lag-time compared to the native LDL was observed with the method 1. The TBARS levels were 3-5 fold higher than in native LDL when methods 1 and 2 were used, but 33% lower in products made by the method 3. The number of thiol groups increased 3 fold in method 1, was only slightly elevated in method 3, but reduced in method 2 compared to native LDL. NH2-groups were increased with all three labeling procedures, but this increase was not considered significant. REM was altered only in products obtained by methods 1 (1.5× increase) and by method 2 (1.25× increase). No fragmentation of Apo B using sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) electrophoresis was observed by 99mTc-LDL produced in any of the methods. The increase of lipid ...
Of 14 strains of Bacillus thuringiensis selected from 956 isolates from soil samples from Brazil, 12 were toxic to larvae of Aedes fluviatilis and two were nontoxic. Nine of the 14 strains were serotyped as subspecies israelensis (serotype 14), one as subspecies kurstaki (serotype 3a 3b) one as subspecies morrisoni (serotype 8a 8b) and three did not agglutinate any antisera. Electrophoresis of whole cell proteins showed that all subsp. israelensis strains formed a homogeneous group which included two non-typable toxic strains, and could be readily distinguished from reference strains toxic for lepidoptera or coleoptera ...
In other words, if you want to be drunk and anti-social and violent until all hours after the OFarrell Government proposals have been passed, all you need to do is pick the right venue in the right inner Sydney precinct. Sure, you can agree or disagree with Robertsons overall stance on the issue, but you cant deny that he too has a point there.. News Limiteds David Penberthy has offered his usual boofhead libertarianism schtick in response. The shorter Penbo: dont blame alcohol, blame the idiots who get violent after a few drinks: you and me are entitled to get pissed as much as we want so long as we dont coward punch anyone. This is the kind of mentality I would ordinarily expect to find at the bar of an RSL after (yep) a few drinks, not splashed all over the HTML and news print produced by Australias largest media company. But then I remember that this is News Limited we are talking about, and that by definition, even companies touting cow manure have a target market. There are some ...
In other words, if you want to be drunk and anti-social and violent until all hours after the OFarrell Government proposals have been passed, all you need to do is pick the right venue in the right inner Sydney precinct. Sure, you can agree or disagree with Robertsons overall stance on the issue, but you cant deny that he too has a point there.. News Limiteds David Penberthy has offered his usual boofhead libertarianism schtick in response. The shorter Penbo: dont blame alcohol, blame the idiots who get violent after a few drinks: you and me are entitled to get pissed as much as we want so long as we dont coward punch anyone. This is the kind of mentality I would ordinarily expect to find at the bar of an RSL after (yep) a few drinks, not splashed all over the HTML and news print produced by Australias largest media company. But then I remember that this is News Limited we are talking about, and that by definition, even companies touting cow manure have a target market. There are some ...
In the next 12 months, the mobile tide will rise in Canada, and dotMobi Advisory Group chair Michael OFarrell has a lot to say about why that is, and how to take advantage of the coming opportunities in mobile marketing.
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This unit contains procedures for electrophoretically transferring proteins onto a variety of membranes including polyvinylidene difluoride (PVDF) and nitrocellulose, and derivatized membranes. The choice of membrane type for electrotransfer is depen