A continuous forced flow electrophoresis cell and a method of operation for the fractionation of an aqueous liquid, such as blood, containing at least two compounds, the relative mobilities of which in an electric field vary as a function of the pH, in order to obtain one fraction enriched and one depleted in one of the compounds, the cell having six compartments divided by ion permeable membranes, the end cell containing an anode and cathode respectively, the central cells being separated by a microporous membrane. The liquid is fed to one of the central cells the filtered fraction being removed, after passage through the microporous membrane, from the other. A main electrolyte is fed to and from the end cells and an auxiliary electrolyte to the intermediate cells such that the pH in one intermediate cell differs from that of the other.
A method for the isolation of brush-border membranes from newborn-rat kidney, employing centrifugation and free-flow electrophoresis, is described. The composition and purity of the preparation was assessed by determination of enzyme activities specific for various cellular membranes. Free-flow electrophoresis resolves the newborn-rat renal membrane suspension into two populations of alkaline phosphatase-enriched brush-border membranes, designated A and B, with the A peak also showing activity of (Na+ + K+)-stimulated ATPase, the basolateral membrane marker enzyme, whereas those of the B peak were enriched 11-fold in alkaline phosphatase and substantially decreased in (Na+ + K+)-stimulated ATPase activity. Membranes in the A peak showed a 7-fold enrichment of alkaline phosphatase, and (Na+ + K+)-stimulated ATPase activity similar to that of the original homogenate. Proline uptake employed to assess osmotic dependency revealed 7% binding of proline to the B vesicles and 31% to the A vesicles. ...
Fig 1. Principle of free-flow electrophoresis. The miniaturization of FFE implies several advantages especially considering sample volume and separation speed. In contrast to the tens of milliliters of sample consumed by conventional large scale FFE devices, microfluidic FFE systems require only tens of nanoliters up to hundreds of microliters of sample. This is especially interesting in clinical analysis where often only low sample volumes are available. Furthermore, instead of residence times of up to tens of minutes, microfluidic FFE (µ-FFE) devices separate within several seconds. The aim of this project is to develop a microfluidic free-flow electrophoresis device (as shown in figure 2 and 3) which eventually might find application in so called proteomics-on-a-chip systems for proteome analysis.. ...
FFE Service GmbH offers the following products: free flow electrophoresis system, an upgrade kit for existing instruments and a FFE robotic system.
FFE Service GmbH conducts Free Flow Electrophoresis training courses, where customers can bring their own sample and elaborate the optimum protocols during the training.
article{5aecc78e-10a8-4a8d-a992-f32f2a699c72, abstract = {Microchip-based free flow acoustophoresis (FFA) in combination with two-dimensional cell prefocusing enables concurrent multiple target outlet fractionation of leukocytes into subpopulations (lymphocytes, monocytes, and granulocytes); we report on this method here. We also observed significantly increased accuracy in size-based fractionation of microbeads as compared to previously presented FFA multiple outlet systems. Fluorescence microscopy illustrates the importance of two-dimensional prefocusing where a sample mixture of 3, 7, and 10 mu m beads are separated into well-confined particle streams and collected in their respective target outlets. Flow cytometry data for lymphocytes and granulocytes, respectively, in their corresponding outlets verify concurrent isolation of leukocyte subpopulations with high purity (95.2 +/- 0.6% and 98.5 +/- 0.7%) and high recovery (86.5 +/- 10.9% and 68.4 +/- 10.6%). A relatively low purity and high ...
May 2, 2007. S. 1267 (110th). A bill to maintain the free flow of information to the public by providing conditions for the federally compelled disclosure of information by certain persons connected with the news media. In GovTrack.us, a database of bills in the U.S. Congress.
Free Flow IPA is a American IPA style beer brewed by Otter Creek Brewing in Middlebury, VT. 4.08 average with 268 ratings, reviews and opinions.
Although the phenomenon we now call dielectrophoresis was described in passing as far back as the early 20th century, it was only subject to serious study, named and first understood by Herbert Pohl in the 1950s.[9][10] Recently, dielectrophoresis has been revived due to its potential in the manipulation of microparticles,[2][4][5][11]nanoparticles and cells. Dielectrophoresis occurs when a polarizable particle is suspended in a non-uniform electric field. The electric field polarizes the particle, and the poles then experience a force along the field lines, which can be either attractive or repulsive according to the orientation on the dipole. Since the field is non-uniform, the pole experiencing the greatest electric field will dominate over the other, and the particle will move. The orientation of the dipole is dependent on the relative polarizability of the particle and medium, in accordance with Maxwell-Wagner-Sillars polarization. Since the direction of the force is dependent on field ...
Microreactors have gained increasing attention in their application toward continuous micro flow synthesis. An unsolved problem of continuous flow synthesis is the lack of techniques for continuous...
This thesis presents two new concepts for separation of micro particles using dielectrophoresis, demonstrated by calculated examples, as well as a new method for obtaining dielectric data on living cells. The thesis is based on four papers.. Paper I describes how the trapping efficiency of micro particles may be significantly increased when superpositioned electric fields are employed in a high conductivity medium. Avoiding low conductivity media is important when working with living cells. Calculations were performed to predict trajectories of Escherichia coli bacteria in the system with superpositioned electric fields, and a model was developed which employed two arrays of interdigitated electrodes in a micro channel.. Paper II proposes a new concept for separation of micro particles, based on repetitive dielectrophoretic trapping and release in a flow system. Calculations show that the resolution increases as a direct function of the number of trap and release steps, and that a difference in ...
Vital Baby Soft Spout Babys 1st Tumbler - a 120ml cup with a soft spout. Perfectly sized for little hands and can be used either non-spill or free flow as your baby develops. Simply remove the 2-in1 non-spill valve from the cap, and the cup can be used as a free-flow cup. With the value in place, liquid will only flow when your baby drinks from the soft spout. Vital Baby cups have soft spouts, and grips that are kind and gentle to your babys sensitive mouth and tiny hands, allowing you to choose the cup that best suits your babys changing needs as it develops. Age range from 4+ months. All Vital Baby cups are dishwasher safe if placed on the top shelf and can be steam sterilised. We really have thought of everything! 1. Both non-spill and free flow drinking 2. Protect your babys sensitive gums with our soft spout 3. Built-in measurement markings to monitor how much liquid baby is drinking 4. Cleverly designed hygienic spout cover 5. 120ml/4oz capacity 6. BPA-Free
Our research takes advantage of Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), a technology that we have extensively modified over the years to give us a suite of methods for comprehensive analysis of glycosylation processes of the ER. These innovations have led to many exciting collaborations with investigators both at UT Southwestern and around the world, involving biological samples from various animals, parasites, plants, and bacteria. Here is a partial list of labs that have recently collaborated with us to use FACE, with PubMed links to Resulting publication that resulted:. ...
That episode was so over the top gross that it is leading me to believe that Shonda is going for the shock factor and no longer confident that her characters stories are strong enough to keep us coming back. All, I know is that I couldnt get through that episode fast enough and the case of that poor man has stayed with me even today. I feel nauseous about it and this is not how I want to feel. If I want to know about the health issues of the indigent people of third world countries, Ill go to National Geographic ...
Tong Ze Bu Tong; Bu Tong Ze Tong" Huang Di Nei Jing "If there is free flow, there is no pain; if there is pain, there is lack of free flow." In Traditional Chinese Medicine, Qi (air or fluid) is stimulated at specific Acupuncture points to create a balanced flow throughout the body, this is said to balance the Yin and Yang of the body and create a smooth flow of [...]. ...
A method of simultaneously forming a multiplicity of surface mountable solid state capacitors is disclosed. The method comprises mounting on a substrate a wafer of powdered solid state capacitor forming metal, sintering the wafer and metal together to fuse the interface between wafer and substrate to each other and convert the wafer into a porous integral mass, dividing the sintered wafer into a multiplicity of mutually spaced sub-units, isolating the interface or boundary between the substrate and sub-units one from the other by a resin infusion or by a dielectric deposition step, causing the sub-units to be converted to capacitors by sequential anodizing and manganizing steps, bonding a cathode plate to the counter electrode components present on the upper surfaces of the sub-units in electrical contact therewith, filling the voids between adjacent sub-units with insulative resin material by injecting same between the plates utilizing the plates as elements of a mold, and after hardening of the resin
Medox-Bio electrophoretic systems are designed to suit various applications related to Nucleic acid separation, Protein seperation, Immuno protein separation and Protein transfer in laboratories. These computer designed systems are made out of well-polished and clear Plexiglass sheets fused together by using high-strength specific adhesives.
An electrophoretic analysis of the dissolved precipitate and supernatant fluid was conducted in the equivalent zone. For microelectrophoresis on paper, a Flynn and De Mayo type of chamber as modified...
Although bacterial culture remains the gold standard for detection of viable bacteria in environmental specimens, the typical time requirement of twenty-four hours can delay and even jeopardize appropriate determination. In addition, culture is incapable of detecting viable but not culturable (VBNC) species. Conversely, nucleic acid and antibody-based methods decrease time to detection but rarely characterize viability of the bacteria detected. Through selection by membrane permeability, the method described in this work employs positive dielectrophoresis (pDEP) for separation and purification of viable and VBNC species from water and allows concentration of bacteria for downstream applications.
Nucleotides are the sub-units that make up DNA, and are made up of even smaller sub-units. The differences between these sub-units result in the unique base pairs that record genetic information and make up genes. More » ...
Electrophoretic variation of proteins encoded by 14 loci was analyzed in eight (five continental and three insular) populations of the Chilean leptodactylid frog Batrachyla leptopus.
Tumor initiating cells (TICs), also known as cancer stem cells, are a finite population of cancer cells that have the ability to transplant a new tumor from an...
Sigma-Aldrich offers abstracts and full-text articles by [Rebecca Soffe, Shi-Yang Tang, Sara Baratchi, Sofia Nahavandi, Mahyar Nasabi, Jonathan M Cooper, Arnan Mitchell, Khashayar Khoshmanesh].
A device for holding an IV tube in a pumping apparatus and for selectively occluding the tube when the door of the pumping apparatus is opened, to prevent free flow of fluid in the tube. The device consists of a base member to which the IV tube is attached or through which it passes, and a slide clamp which slides relative to the base member and relative to the tube. The IV tube is passed through an elongated aperture in the slide clamp, and the slide clamp is slidingly mounted on the base member. The aperture in the slide clamp has a relatively open hole at one end, through which the tube passes when the slide clamp is in one position, allowing fluid to flow through the tube. The aperture also has a relatively constricted slot at the other end, through which the tube passes when the slide clamp is in a second position, preventing fluid flow through the tube. A flexible cantilevered arm on the base interlocks with a locking projection on the slide clamp to lock the slide clamp in the second
Body plan: molds Hyphae - long filaments that invade their food source and provide a large surface area to absorb nutrients Mycelium - a tangled mat of hyphae Some have cells separated by cell walls called septa, others do not - coenocytic Even those with septa often have large pores that allow free flow of organelles between cells
Build confidence as your toddler grows and needs larger amounts of liquid. Its soft spout, easy grip base and large 280ml capacity make our Toddler Trainer Cup perfect for this. It can be used as non spill or free flow. Our award winning range of Vital Baby® Trainer Cups is specially designed to protect sensitive gums, teach how to hold, build confidence and ultimately help to develop baby s drinking skills as they grow. Every cup comes complete with a non-spill valve which has been cleverly designed to provide a smooth drinking action and a steady flow of liquid from just 4 months old!
Earth - Spleen-Pancreas/Stomach - is associated with the harvest time. It is the patient and nourishing mediator, it represents balance and neutrality. The colour is yellow, the flavour bland or sweet and the negative emotions are worry and over thinking; positive is fairness and openness - and singing is associated with the free flow of spleen Chi. You can learn more by watching the following short video. The Five Phases correspond to each other through nourishing and controlling cycles. Metal nourishes Water, Water nourishes Wood, Wood nourishes Fire, Fire nourishes Earth, Earth nourishes Metal ...
Talk of such policies is often met by objections that this will lead to a dangerous balkanisation of global capital markets, preventing the free flow of capital and stymieing its allocative efficiency benefits," he said. "But since the evidence for the benefits of financial integration is at best elusive and ambiguous, some balkanisation of short term international debt markets could be a good thing ...
WHAT CAUSES SNORING? The noisy sounds of snoring occur when there is an obstruction to the free flow of air through the passages at the back of the mouth
This report is chilling in its implications on the free flow of information to the American people. Under the bill, which is written in extremely broa
Snoring is most annoying health situation, snoring, it is a noise reason when there is an obstacle to the free flow of air through the passages at the back of the mouth and nose. That area is the collapsible part of the airway. Snoring happens when these structures hit each other and vibrate during breathing.. ...
Hubby saya memang dah nak try makan kat restoran ni masa dia datang ke JB sebelum tu tapi tak berani dan tak best sorang2...sbb dia menyediakan hidangan steamboat dan BBQ secara buffet. Alhamdulillah amat puasa hati makan kat sini. Pilihannya amatlah banyak berpuluh2 jenis rasanya...macam2 ada,ayam lebih 10 jenis, daging lembu, daging kambing dan ikan ,pelbagai jenis fishballs, meatballs, udang, sotong, kerang, mussels, sayur, dessert (aiskrim, ABC, cendol, buah-buahan etc) dan air yang free flow (soft drink, juice). Harga RM70++ untuk dua orang ...
Advanced search is divided into two main parts, and one or more groups in each of the main parts. The main parts are the "Search for" (including) and the "Remove from search" (excluding) part. (The excluding part might not be visible until you hit "NOT" for the first time ...
Definition : Tubes covered with supporting media that are used to separate an ionic mixture of solutes into its components by the differences in migration rate when an electric field is applied (electrophoresis). These columns are usually made of glass or plastic (e.g., acrylic) tubes; they are usually mounted vertically, with both ends placed into buffer wells that make the electrical connection between the columns and the electrodes. Typically, several columns are run simultaneously in an electrophoretic system.. Entry Terms : "Columns, Electrophoresis" , "Immunoelectrophoresis Equipment" , "Apparatus, Electrophoresis" , "Electrophoresis Equipment" , "Chromatography Equipment". UMDC code : 18551 ...
Mulhall, HJ, Abdallat, R, Liang, X, Fedele, S, Lewis, MP, Porter, S, Tsinkalovsky, O, Johannessen, AC, Hughes, MP, Costea, DE and Labeed, FH (2010) Rapid detection of oral cancer: Electrophysiological characterization by dielectrophoresis technology In: Nanotech Conference & Expo 2010, 2010-06-21 - 2010-06-24, Anaheim, USA. Armellin, R, Lavagna, M, Starkey, RP and Lewis, MJ (2006) Aero-gravity assist maneuvers: Coupled trajectory and vehicle shape optimization In: AIAA/AAS Astrodynamics Specialist Conference and Exhibit, 2006-08-21 - 2006-08-24, Keystone, U.S.A.. ...
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Article: Smith, James P.; Huang, Chao; Kirby, Brian J.; (2015) "Enhancing Sensitivity and Specificity in Rare Cell Capture Microdevices with Dielectrophoresis", Biomicrofluidics, 9(1). DOI. Abstract: The capture and subsequent analysis of rare cells, such as circulating tumor cells from a peripheral blood sample, has the potential to advance our understanding and treatment of a wide range of diseases. There is a particular need for high purity (i.e., high specificity) techniques to isolate these cells, reducing the time and cost required for single-cell genetic analyses by decreasing the number of contaminating cells analyzed. Previous work has shown that antibody-based immunocapture can be combined with dielectrophoresis (DEP) to differentially isolate cancer cells from leukocytes in a characterization device. Here, we build on that work by developing numerical simulations that identify microfluidic obstacle array geometries where DEP-immunocapture can be used to maximize the capture of target ...
Cavalli, sforza L.; Santachiara, S A.; Wang, L; Erdelyi, E; and Barchas, J, "Electrophoretic study of 5-hydroxytryptophan decarboxylase from brain and liver in several species." (1974). Subject Strain Bibliography 1974. 607 ...
TY - GEN. T1 - Dielectrophoresis of DNA using 3D Carbon Electrodes. T2 - Electrophoresis. AU - Martinez-Duarte, R. AU - Camacho-Alanis, F. AU - Renaud, P. AU - Ros, A. PY - 2013. Y1 - 2013. M3 - Other contribution. ER - ...
Mote B. Normal serum protein values in newborn infants, determined by cellulose acetate electrophoresis. J Am Osteopath Assoc 1967;67(4):430. doi: https://doi.org/.. Download citation file:. ...
Carbon nanomaterials have a large surface area and complex particles shape architecture. These qualities are successfully applying to develop new drug carriers, fillers, or nanodetectors [1]. At the same time, the utilization efficiency of such materials is caused by maintaining of the nanodispersity. An agglomeration and partially sedimentation of nanotubes in aqueous media is a well-known fact. The stability of the nanotube dispersion mostly depends on the uniformity of the boundary layer and nanotube morphology. The hydrophobic nature of defectless areas of their surface and the hydrophilicity of some types of defects in the nanotube boundary layer, namely carboxy- or phenolic groups [2, 3] which are formed during nanotube purification and washing, are competing during dispersing in the aqueous media. An increase in stability of the nanotube dispersion in water can be achieved through the surface charge via formation of the double electrical layer (EDL). Among other carbon materials, carbon ...
Fluid-Screen, Inc. presents a bacterial concentration and filtration method based on dielectrophoresis (DEP). DEP has been known to induce particle motion since the 1960s. However, yields and reproducibility have consistently been low in practice, reducing the potential of DEP for practical applications. Here we present a novel electrode design to induce high electric field gradients. This design allows rapid and efficient capture of diverse bacteria from aqueous solutions of varied conductivity. By using the novel spiral electrode design and tuning the electric field to species-specific frequencies, we show capture of over 99% of bacteria within seconds of gradient application. Our method of DEP induces responses in both the representative Gram-negative Escherichia coli and Gram-positive Enterococcus faecalis bacteria. Equilibrium repositioning of suspended bacteria can be seen within miliseconds for low-conductivity medium and within seconds for higher conductivity solutions such as environmental
Development and Validation of a Simple and Rapid Capillary Zone Electrophoresis Method for Quantification of Heparin in a Pharmaceutical Product and Stability Studies Abstract.
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The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transmitted through the chamber, it exits at the opposite end of the chamber at a characteristic position. Methods according to
The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exists at the opposite end of the chamber at a characteristic position. Methods according to
URN zum Zitieren dieses Dokuments: urn:nbn:de:bvb:355-epub-219889. Hansen, Ernil und Hannig, Kurt (1984) Electrophoretic separation of lymphoid cells. Methods in enzymology 108, S. 180-197 ...
In order to point out the driven forces in the loading and release of protein onto thermally sensitive poly(N-isopropylacrylamide) (NIPAM) nanogel particles, which largely claimed in drug delivery and analytical processes, various polyNIPAM based nanogel particles were prepared, and the loading and release of two model proteins: bovine serum albumin (BSA) and -Globulins (G), onto various cationically or anionically charged polyNIPAM nanocrogel particles were investigated. At first, the nanogels particles were well characterized regarding their colloidal properties (swelling ability, particle size, and electrokinetic properties). Then, the loading and release of proteins behaviour onto the elaborated particles were investigated as a function of temperature, pH, and salinity. It was found that the loaded amount of both proteins increases as a function of temperature irrespective of the nature of charge of polyNIPAM nanogels. The protein loaded amount onto the two lower charged polyNIPAM ...
This thesis presents two new concepts for separation of micro particles using dielectrophoresis, demonstrated by calculated examples, as well as a new method for obtaining dielectric data on living cells. The thesis is based on four papers.. Paper I describes how the trapping efficiency of micro particles may be significantly increased when superpositioned electric fields are employed in a high conductivity medium. Avoiding low conductivity media is important when working with living cells. Calculations were performed to predict trajectories of Escherichia coli bacteria in the system with superpositioned electric fields, and a model was developed which employed two arrays of interdigitated electrodes in a micro channel.. Paper II proposes a new concept for separation of micro particles, based on repetitive dielectrophoretic trapping and release in a flow system. Calculations show that the resolution increases as a direct function of the number of trap and release steps, and that a difference in ...
An activated carbon fiber composite for separation and purification, or catalytic processing of fluids is described. The activated composite comprises carbon fibers rigidly bonded to form an open, permeable, rigid monolith capable of being formed to near-net-shape. Separation and purification of gases are effected by means of a controlled pore structure that is developed in the carbon fibers contained in the composite. The open, permeable structure allows the free flow of gases through the monolith accompanied by high rates of adsorption. By modification of the pore structure and bulk density the composite can be rendered suitable for applications such as gas storage, catalysis, and liquid phase processing.