Early Growth Response Transcription Factors: A family of transcription factors that are induced by GROWTH FACTORS and contain a highly conserved DNA-binding domain composed of three ZINC FINGER MOTIFS.

TY - JOUR. T1 - TGF-β inducible early gene 1 regulates osteoclast differentiation and survival by mediating the NFATc1, AKT, and MEK/ERK signaling pathways. AU - Cicek, Muzaffer. AU - Vrabel, Anne. AU - Sturchio, Catherine. AU - Pederson, Larry. AU - Hawse, John R.. AU - Subramaniam, Malayannan. AU - Spelsberg, Thomas C.. AU - Oursler, Merry Jo. PY - 2011. Y1 - 2011. N2 - TGF-β Inducible Early Gene-1 (TIEG1) is a Krüppel-like transcription factor (KLF10) that was originally cloned from human osteoblasts as an early response gene to TGF-β treatment. As reported previously, TIEG1-/- mice have decreased cortical bone thickness and vertebral bone volume and have increased spacing between the trabeculae in the femoral head relative to wildtype controls. Here, we have investigated the role of TIEG1 in osteoclasts to further determine their potential role in mediating this phenotype. We have found that TIEG1-/- osteoclast precursors differentiated more slowly compared to wildtype precursors in ...

Early Growth Response Protein 1: An early growth response transcription factor that has been implicated in regulation of CELL PROLIFERATION and APOPTOSIS.

Transcriptional regulator. Recognizes and binds to the DNA sequence 5-GCG(T/G)GGGCG-3(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death.

Transcriptional regulator. Recognizes and binds to the DNA sequence 5-GCGGGGGCG-3 (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation (By similarity).

Other researchers who were studying kidney development previously identified transcription factor KLF12 as a transcriptional repressor of the AP-2α gene. It was then discovered that AP-2α expression is also reduced in advanced CRC tumor tissue compared to matched normal tissue and that loss of AP-2α promoted CRC invasion. This connection illuminated a potential link between KLF12 and CRC. In vitro studies show that KLF12 promotes gastric cancer (GC) cell proliferation and invasion, and that KLF12 levels are elevated in about 40% of poorly differentiated GCs and correlate with tumor size. Furthermore, recent genome-wide analyses find high KLF12 levels in approximately 40% of esophageal adenocarcinomas and in 45% of salivary tumors. Until now, however, the role of KLF12 in CRC remained unclear.. The MUSC research team designed a series of in vitro and in vivo experiments to clarify the role of KLF12 in CRC. The first set of studies examined KLF12 expression in 7 human CRC cell lines. They found ...

Transcriptional regulator (PubMed:20121949). Recognizes and binds to the DNA sequence 5-GCG(T/G)GGGCG-3(EGR-site) in the promoter region of target genes (By similarity). Binds double-stranded target DNA, irrespective of the cytosine methylation status (PubMed:25258363, PubMed:25999311). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (By similarity ...

Microglial hyperactivity contributes to neuronal damage resulting from CNS injury and disease. However although P2X7 receptor activation is well recognized to regulate processing and release of cytokines little is known concerning its role in regulating the Cladribine transcription of inflammatory genes nor the molecular mechanisms underlying these transcriptional effects. In the present studies we identify that the transcription factors early growth response (Egr)-1 -2 and -3 are downstream signaling targets of P2X7 receptors in microglia and that their activation is sensitive to MEK and p38 mitogen-activated protein kinase (MAPK) inhibitors. Moreover using RNAi we demonstrate that Egr factors and P2X7 receptors are necessary for BzATP-mediated attenuation of iNOS and stimulation of TNF-α and IL-6 gene expression. BzATP also attenuates neuronal death induced by LPS conditioned medium and P2X7 receptors are required for this effect. These studies are the first to identify Egr factors as ...

The periodic formation of plant organs such as leaves and flowers gives rise to intricate patterns that have fascinated biologists and mathematicians alike for hundreds of years. The plant hormone auxin plays a central role in establishing these patterns by promoting organ formation at sites where it accumulates due to its polar, cell-to-cell transport. Although experimental evidence as well as modeling suggest that feedback from auxin to its transport direction may help specify phyllotactic patterns, the nature of this feedback remains unclear. Here we reveal that polarization of the auxin efflux carrier PIN-FORMED 1 (PIN1) is regulated by the auxin response transcription factor MONOPTEROS (MP). We find that in the shoot, cell polarity patterns follow MP expression, which in turn follows auxin distribution patterns. By perturbing MP activity both globally and locally, we show that localized MP activity is necessary for the generation of polarity convergence patterns and that localized MP ...

A sandwich ELISA kit for quantitative measurement of Mouse EGR1 (Early Growth Response Protein 1) in samples from Serum, Plasma, Cell supernatant

TY - JOUR. T1 - Early growth response gene 1 (EGR1) regulates heparanase gene transcription in tumour cells. AU - De Mestre, Amanda. AU - Rao, Sudha. AU - Hornby, June. AU - Soe-Htwe, Thura. AU - Khachigian, Levon. AU - Hulett, Mark. PY - 2005/10/21. Y1 - 2005/10/21. N2 - Heparanase is an endoglycosidase that degrades heparan sulfate chains of heparan sulfate proteoglycans, a key component of extracellular matrix and basement membranes. Studies using heparanase inhibitors and gene silencing have provided evidence to support an important role for heparanase in tumor metastasis and angiogenesis. The expression of heparanase is normally very tightly controlled, however, it is commonly deregulated in tumor cells, which express elevated heparanase activity that correlates with high levels of heparanase mRNA. We recently identified the transcription factor early growth response gene 1, EGR1, as a key regulator of inducible heparanase transcription in T cells. In this study using chromatin ...

Summary: Medical University of South Carolina (MUSC) investigators report preclinical research showing that Krüppel-like factor 12 (KLF12) promotes colorectal cancer (CRC) cell growth by activating early growth response protein 1 (EGR1), in the July 2016 issue of PLOS One. Data also reveal that levels of KLF12 and EGR1 correlate synergistically with a poor CRC prognosis. Results indicate that KLF12 plays an important role in CRC progression and provides a potential novel prognostic marker and therapeutic target.. Results of preclinical studies by MUSC investigators reported in the July 2016 issue of PLOS One (doi:10.1371/journal.pone.0159899) demonstrate for the first time that the transcription factor Krüppel-like factor 12 (KLF12) promotes poor colorectal cancer (CRC) cell growth, in part, by activating EGR1. Furthermore, data demonstrate that KLF12 and early growth response protein 1 (EGR1) levels synergistically correlate with CRC prognoses.. CRC is the third most common and third ...

Dr. Zhao is interested in neurodegenerative and neuropsychiatric disorders research. In her graduate school in Arizona State University, she worked in Dr. Steve Macknics lab and found that hypoxia, caused by capillary constrictions in the hippocampus, contributes to neurodegeneration in epileptic mouse model 1. She completed her Ph.D. dissertation project in Dr. Amelia Gallitanos lab. She found that the transcription factor early growth response 3 (EGR3) regulates the serotonin 2A receptor gene (Htr2a) probably through both direct and indirect ways 2,3. Identification of the mechanism could provide information for the proposed regulatory network which integrates genetic and environmental factors influences on schizophrenia and could provide future therapeutic targets to diagnose and treat schizophrenia. Currently, she is working on how the aging process contributes to changes in neural stem cell activity in the subventricular zone.. Publications:. ...

Adhesion of Human Osteoblasts Cell on TiN Thin Film Deposited by Cathodic Arc Plasma Deposition - Human osteoblast;TiN film;Cell adhesion;Surface modification;Cytoskeleton;

TY - JOUR. T1 - Inositol polyphosphate multikinase is a coactivator of p53-mediated transcription and cell death. AU - Xu, Risheng. AU - Sen, Nilkantha. AU - Paul, Bindu D.. AU - Snowman, Adele M.. AU - Rao, Feng. AU - Vandiver, M. Scott. AU - Xu, Jing. AU - Snyder, Solomon H.. PY - 2013/4/2. Y1 - 2013/4/2. N2 - The tumor suppressor protein p53 is a critical stress response transcription factor that induces the expression of genes leading to cell cycle arrest, apoptosis, and tumor suppression. We found that mammalian inositol polyphosphate multikinase (IPMK) stimulated p53-mediated transcription by binding to p53 and enhancing its acetylation by the acetyltransferase p300 independently of its inositol phosphate and lipid kinase activities. Genetic or RNA interference (RNAi)-mediated knockdown of IPMK resulted in decreased activation of p53, decreased recruitment of p53 and p300 to target gene promoters, abrogated transcription of p53 target genes, and enhanced cell viability. Additionally, ...

The Caenorhabditis elegans oxidative stress response transcription factor, SKN-1, is essential for the maintenance of redox homeostasis and is a functional ortholog of the Nrf family of transcription factors. The numerous levels of regulation that govern these transcription factors underscore their importance. Here, we add a thioredoxin, encoded by trx-1, to the expansive list of SKN-1 regulators. We report that loss of trx-1 promotes nuclear localization of intestinal SKN-1 in a redox-independent, cell non-autonomous fashion from the ASJ neurons. Furthermore, this regulation is not general to the thioredoxin family, as two other C. elegans thioredoxins TRX-2 and TRX-3 do not play a role in this process. Moreover, TRX-1-dependent regulation requires signaling from the p38 MAPK signaling pathway. However, while TRX-1 regulates SKN-1 nuclear localization, classical SKN-1 transcriptional activity associated with stress response remains largely unaffected. Interestingly, RNA-Seq analysis revealed ...

Background Within the scanning model of translation initiation, reinitiation is a non-canonical mechanism that operates on mRNAs harboring upstream open reading frames. The h subunit of eukaryotic initiation factor 3 (eIF3) boosts translation reinitiation on the uORF-containing mRNA coding for the Arabidopsis bZip transcription factor, AtbZip11, among others. The RPL24B protein of the large ribosomal subunit, which is encoded by SHORT VALVE1, likewise fosters translation of uORF-containing mRNAs, for example mRNAs for auxin response transcription factors (ARFs). Results Here we tested the hypothesis that RPL24B and eIF3h affect translation reinitiation in a similar fashion. First, like eif3h mutants, rpl24b mutants under-translate the AtbZip11 mRNA, and the detailed spectrum of translational defects in rpl24b is remarkably similar to that of eif3h. Second, eif3h mutants display defects in auxin mediated organogenesis and gene expression, similar to rpl24b. Like AtbZip11, the uORF-containing ARF mRNAs

Fungi cause disease in plant and animal hosts. The extent to which virulence determinants are conserved between both classes of pathogens is unknown. We have developed a dual plant-animal infection model based on a single strain of Fusarium oxysporum, the causal agent of vascular wilt disease in plants and an emerging opportunistic pathogen of humans. Injection of microconidia of the well-characterized tomato pathogenic isolate 4287 in the lateral tail vein of immunodepressed mice resulted in disseminated infection of multiple organs and death of the animals. Knockout mutants in genes encoding a Pmk1-type mitogen-activated protein kinase, the pH response transcription factor PacC or a class V chitin synthase, all previously shown to be implicated in virulence on tomato plants, were tested in the disseminated mouse model. Our results indicate that some of these virulence factors play functionally distinct roles during infection of tomato and mice. Thus, a single F. oxysporum strain can be used to ...

Participates as a hydrogen donor in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, accompanied by the transfer of 2 electrons and 2 protons. It is involved in many cellular processes, including deoxyribonucleotide synthesis, repair of oxidatively damaged proteins, protein folding, sulfur metabolism, and redox homeostasis. Thioredoxin-dependent enzymes include phosphoadenosine-phosphosulfate reductase MET16, alkyl- hydroperoxide reductase DOT5, thioredoxin peroxidases TSA1 and TSA2, alkyl hydroperoxide reductase AHP1, and peroxiredoxin HYR1. Thioredoxin is also involved in protection against reducing stress. As part of the LMA1 complex, it is involved in the facilitation of vesicle fusion such as homotypic vacuole and ER- derived COPII vesicle fusion with the Golgi. This activity does not require the redox mechanism. Through its capacity to inactivate the stress response transcription factor YAP1 and its regulator the hydroperoxide stress sensor ...

EGR1 (Early Growth Response 1), Authors: Reeti Bandyopadhyay, Véronique Baron. Published in: Atlas Genet Cytogenet Oncol Haematol.

This study characterised the effects of persistent peripheral inflammation of the foot on pain and spinal cord expression of cyclooxygenase-1 and -2 (COX-1 and COX-2) and early growth response gene 1 (Egr-1), known markers of neuronal plasticity, in a clinical model of naturally-occurring inflammatory disease and hyperalgesia in sheep (footrot), before and after routine treatment (parenteral treatment with antibiotics and antiseptic footbathing). The temporal pattern of expression of COX-1, COX-2 and Egr-1 mRNA and protein were analysed using real-time PCR and Western blotting. Animals affected with persistent peripheral inflammation displayed significant hyperalgesia and lameness (a proxy indicator of spontaneous pain) restricted to the inflamed limb. Hyperalgesia and lameness were significantly attenuated 1 day after treatment, and resolved further by day 7 and day 3, respectively. COX-2 but not COX-1, protein expression was up-regulated in spinal cord from lame animals on day 0, before treatment.

Pollination in flowering plants requires that anthers release pollen when the gynoecium is competent to support fertilization. We show that in Arabidopsis thaliana, two paralogous auxin response transcription factors, ARF6 and ARF8, regulate both stamen and gynoecium maturation. arf6 arf8 double-null mutant flowers arrested as infertile closed buds with short petals, short stamen filaments, undehisced anthers that did not release pollen and immature gynoecia. Numerous developmentally regulated genes failed to be induced. ARF6 and ARF8 thus coordinate the transition from immature to mature fertile flowers. Jasmonic acid (JA) measurements and JA feeding experiments showed that decreased jasmonate production caused the block in pollen release, but not the gynoecium arrest. The double mutant had altered auxin responsive gene expression. However, whole flower auxin levels did not change during flower maturation, suggesting that auxin might regulate flower maturation only under specific envi

The worlds first wiki where authorship really matters. Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts.

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Complete information for EGR1 gene (Protein Coding), Early Growth Response 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

Wilms tumor protein is a protein that in humans is encoded by the WT1 gene on chromosome 11p. This gene encodes a transcription factor that contains four zinc finger motifs at the C-terminus and a proline / glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a subset of patients with Wilms tumor, the genes namesake. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. The WT1 gene product shows similarity to the zinc fingers of the mammalian growth regulated early growth response protein 1 (EGR1) and (EGR2) proteins. Wilms tumour tumor suppressor gene1 (WT1) causes an embryonic malignancy of the kidney, affecting around 1 in 10,000 infants. It occurs in both sporadic and ...

A cellular transcriptional coactivator that was originally identified by its requirement for the stable assembly IMMEDIATE-EARLY PROTEINS of the HERPES SIMPLEX VIRUS. It is a nuclear protein that is a transcriptional coactivator for a number of transcription factors including VP16 PROTEIN; GA-BINDING PROTEIN; EARLY GROWTH RESPONSE PROTEIN 2; and E2F4 TRANSCRIPTION FACTOR. It also interacts with and stabilizes HERPES SIMPLEX VIRUS PROTEIN VMW65 and helps regulate GENETIC TRANSCRIPTION of IMMEDIATE-EARLY GENES in HERPES SIMPLEX VIRUS ...

TY - JOUR. T1 - Isolation of a human homolog of osteoclast inhibitory lectin that inhibits the formation and function of osteoclasts. AU - Yun, Shan Hu. AU - Zhou, Hong. AU - Myers, Damian. AU - Quinn, Julian M W. AU - Atkins, Gerald J.. AU - Ly, Chi. AU - Gange, Christine. AU - Kartsogiannis, Vicky. AU - Elliott, Jan. AU - Kostakis, Panagiota. AU - Zannettino, Andrew C W. AU - Cromer, Brett. AU - Mckinstry, William J.. AU - Findlay, David M.. AU - Gillespie, Matthew T.. AU - Kong, Wah Ng. PY - 2004/1/1. Y1 - 2004/1/1. N2 - Osteoclast inhibitory lectin (OCIL) is a newly recognized inhibitor of osteoclast formation. We identified a human homolog of OCIL and its gene, determined its regulation in human osteoblast cell lines, and established that it can inhibit murine and human osteoclast formation and resorption. OCIL shows promise as a new antiresorptive. Introduction: Murine and rat osteoclast inhibitory lectins (mOCIL and rOCIL, respectively) are type II membrane C-type lectins expressed by ...

While other growers may dip in and out of barley, Philip Hirst has grown it for as long as he can remember. Barleys always been there. Im not a fan of

TIS21(/BTG2/PC3), orthologs of mouse, human and rat, respectively, is initially identified as one of the early growth response genes and induced by various stimulations. TIS21 belongs to antiproliferative (APRO) gene family containing the BTG-Box A (Y(50)-N(71)) and BTG-Box B (L(97)-E(115)), which are highly conserved among various species. On the other hand, it has lately been found that the expression of TIS21 is constitutive and high in thymus, lung alveolar epithelium, proximal tubule of kidney and basal cell layer of prostate acini. Potential roles of TIS21 have been suggested as transcriptional co-regulator, differentiation and antiapoptotic factor in neurogenesis, key mediator of the stage-specific expansion of thymocyte and negative regulator of hematopoietic progenitor expansion, and tumor suppressor gene in both mouse and human. In addition, as pan-cell cycle regulator TIS21 induces G1/S arrest by pRB dependently and pRB independently and G2/M arrest and cell death in the p53 null ...

Mon., April 30, 11 a.m. Genetic Alterations in Pancreatic Cancer, a Center for Inherited Disease Research seminar with Scott Kern; Suite 2000, Triad Bldg., 333 Cassell Dr. JHMI Mon., April 30, noon. From Books to Bytes: Access and Fair Use in the Digital Environment, an Information Security Institute seminar with James Neal. Part of the Spring 2001 Policy Seminar Series. Glass Pavilion, Levering. HW. Mon., April 30, noon. Egr Proteins in Neuronal Apoptosis, a Neuroscience seminar with Jay Baraban; 1-191 Meyer. JHMI Mon., April 30, noon. The Politics of Budget Surpluses: Can We Afford to Grow Old? an Institute for Policy Studies seminar with Paul Posner, U.S. General Accounting Office; 526 Wyman Park Bldg. HW. Mon., April 30, 12:15 p.m. Determinants of Positive Outcomes for Children, a Population and Family Health/Population Center joint seminar with Kristin Moore, Child Trends Inc.; W2030 SPH. JHMI. Mon., April 30, 12:15 p.m. Chaperoning Cell-Fate Determination in Volvox, a Carnegie ...

1 March 1996 Amino response protein snagsDAIRY cows fed protected amino acids methionine and lysine are failing to give the expected response in milk prote

98 cl 3.0 v6 egr check engin light i replace oxygen sensor and cat and muffler and egr and the idle is still rough - Acura 1997 CL question

Osteosarcoma is the most common primary malignant bone tumor that most frequently affects young adults. There is increasing evidence that miRNAs (miRs) are important modulators of gene expression and their deregulation has been reported in various human tumors. This study investigated differences in miR expression in normal human osteoblast cells versus malignant human osteosarcoma cells differ in their miR expression. Analysis of miRNA expression in normal osteoblast cell lines (hOBc, NHOST, hFOB) and osteosarcoma cell lines (KHOS, Saos, U-2OS) were completed using miR microarray technology with unsupervised hierarchical clustering analysis. The relative expression levels of these miRs were confirmed by real-time quantitative RT-PCR. A number of miRs were found to have different expression in the osteosarcoma cells when compared to normal human osteoblasts. Three miRs, miR-199a-3p, miR-127-3p and miR-376c were significantly decreased in osteosarcoma cell lines while miR-151-3p and miR-191 were ...

Publishers Accepted Manuscript: Incorporation of new particle formation and early growth treatments into WRF/Chem: Model improvement, evaluation, and impacts of anthropogenic aerosols over East Asia ...