BacMap
Salmonella enterica subsp. enterica serovar Newport. Salmonella enterica subsp. enterica serovar Newport is common worldwide. Outbreak investigations and targeted studies have identified dairy cattle as the main reservoir this serotype. Antimicrobial resistance (Newport MDR-AmpC) is particularly problematic in this serotype, and the prevalence of Newport MDR-AmpC isolates from humans in the United States has increased from 0% during 1996-1997 to 26% in 2001. MDR strains have been recorded as resistant to ampicillin, chloramphenicol, streptomycin, sulphonamides and tetracycline (ACSSuT) and many of these strains show intermediate or full resistance to third-generation cephalosporins, kanamycin, potentiated sulphonamides, and gentamicin. Salmonella enterica subsp. enterica serovar Newport str SL254. The SL254 strain is an MDR strain from one of two distinct lineages of the Newport serovar. (NCBI BioProject: bp_list[1 ...
Population Screening Using Sewage Reveals Pan-Resistant Bacteria in Hospital and Community Samples. | Physicians Weekly for...
The presence of pan-resistant bacteria worldwide possesses a threat to global health. It is difficult to evaluate the extent of carriage of resistant bacteria in the population. Sewage sampling is a possible way to monitor populations. We evaluated the presence of pan-resistant bacteria in Israeli sewage collected from all over Israel, by modifying the pour plate method for heterotrophic plate count technique using commercial selective agar plates. This method enables convenient and fast sewage sampling and detection. We found that sewage in Israel contains multiple pan-resistant bacteria including carbapenemase resistant Enterobacteriacae carrying blaKPC and blaNDM-1, MRSA and VRE. blaKPC carrying Klebsiella pneumonia and Enterobacter cloacae were the most common Enterobacteriacae drug resistant bacteria found in the sewage locations we sampled. Klebsiella pneumonia, Enterobacter spp., Escherichia coli and Citrobacter spp. were the 4 main CRE isolated from Israeli sewage and also from clinical ...
Clinical outcomes of intestinal transplant recipients colonized with multidrug-resistant organisms: a retrospective study<...
TY - JOUR. T1 - Clinical outcomes of intestinal transplant recipients colonized with multidrug-resistant organisms. T2 - a retrospective study. AU - Simkins, Jacques. AU - Morris, Michele I.. AU - Camargo, Jose F.. AU - Vianna, Rodrigo. AU - Beduschi, Thiago. AU - Abbo, Lilian M.. PY - 2017/9/1. Y1 - 2017/9/1. N2 - Rates of multidrug-resistant organisms (MDRO) colonization among intestinal transplant (ITx) recipients have not been reported. Colonization rates with vancomycin-resistant Enterococcus (VRE), carbapenem-resistant Gram-negative bacteria (CR-GNB), and methicillin-resistant Staphylococcus aureus (MRSA) were obtained retrospectively in adults undergoing ITx (isolated or multivisceral) from 1/2009 to 12/2015. We assessed for VRE, CR-GNB, and MRSA bacteremia during the first year post-transplant for patients colonized with VRE, CR-GNB, and MRSA, respectively, and for those who were not colonized. We evaluated whether the number of hospitalization days and one year post-transplant survival ...
VARIOUS MEDICINAL PLANTS: A PROMISING TREATMENT FOR MULTIDRUG-RESISTANT BACTERIA ISOLATED FROM WOUND INFECTION | INTERNATIONAL...
INTRODUCTION: The term multidrug-resistants (MDR) applies to the bacterium that is simultaneously resistant to some antimicrobials belonging to different chemical classes 1-3.. Furthermore, Antibiotic resistance is a global challenge that impacts all pharmaceutically used antibiotic.. In recent years pharmaceutical companies have almost stopped producing new antibiotics which have led researchers to look for alternative antimicrobial. Herbs widely use for the treatment of infectious diseases in many developing countries 4. Therefore, in Sudan, with a high percentage of multidrug-resistant bacteria, we in urgent need to develop a new drug from our traditional medicine.. A wide range of medicinal plant parts use for extract as unprocessed drugs, and they possess various medicinal properties. The secondary metabolism of the plant was found to be a source of various phytochemicals that could directly be used as intermediates for the production of new drugs 5-8.. Nosocomial infection is one of the ...
Researchers use novel phage therapy to treat patient infected with multidrug-resistant bacterium
Scientists and physicians at University of California San Diego School of Medicine, working with colleagues at the U.S. Navy Medical Research Center - Biological Defense Research Directorate, Texas A&M University, a San Diego-based biotech and elsewhere, have successfully used an experimental therapy involving bacteriophages -- viruses that target and consume specific strains of bacteria -- to treat a patient near death from a multidrug-resistant bacterium.
Pre GI: SWBIT SVG BLASTN
General Information: The SL254 strain is an MDR strain from one of two distinct lineages of the Newport serovar. Salmonella enterica subsp. enterica serovar Newport is common worldwide. Outbreak investigations and targeted studies have identified dairy cattle as the main reservoir this serotype. Antimicrobial resistance (Newport MDR-AmpC) is particularly problematic in this serotype, and the prevalence of Newport MDR-AmpC isolates from humans in the United States has increased from 0% during 1996-1997 to 26% in 2001. MDR strains have been recorded as resistant to ampicillin, chloramphenicol, streptomycin, sulphonamides and tetracycline (ACSSuT) and many of these strains show intermediate or full resistance to third-generation cephalosporins, kanamycin, potentiated sulphonamides, and gentamicin. This group of Enterobactericiae have pathogenic characteristics and are one of the most common causes of enteric infections (food poisoning) worldwide. They were named after the scientist Dr. Daniel ...
Antibiotic Resistance
This page includes the following topics and synonyms: Antibiotic Resistance, Antibiotic Resistant Infection, Antimicrobial Resistance, Multidrug-Resistant Organism, Multi-Drug Resistant Bacteria.
Drug-Resistant Bacteria Lethal In Hospital Acquired Pneumonia | Medpage Today
PORTO ALEGRE, Brazil -- Hospital-acquired pneumonia caused by multidrug resistant strains of Pseudomonas bacteria are frequently lethal.
RCSB PDB - 3OQA: Crystal Structures of Multidrug-Resistant Clinical Isolate 769 HIV-1 Protease Variants Macromolecule...
UTSW study identifies a way to prevent burn injury infection -- without antibiotics | EurekAlert! Science News
A new way to fight multidrug-resistant bacteria by blinding them rather than killing them proved highly effective in a model of burn injuries, UT Southwestern Medical Center research shows.
Course Content - #94213: Multidrug-Resistant Microbial Infections - NetCE
Today, a generation of microbes has emerged that are so resistant to available medications that they might again become serious threats. Even the once easily treated organisms, such as staphylococcus and streptococcus, have acquired resistance to many standard antimicrobials, making them much harder to treat. The urgency of this situation has prompted the Centers for Disease Control and Prevention (CDC) to issue guidelines that might help keep the situation under control, if universally followed. These guidelines include the concept that the proliferation of resistant organisms can be diminished if good sanitary practices are adhered to. This course will provide an overview of the mechanics of multidrug resistance and the most common resistant organisms.
Regulations Archives - Infectious Diseases Hub
The US FDA has issued a safety communication regarding investigational FMT, warning that there is a risk of serious infection caused by the transfer of multidrug-resistant organisms. ...
A 63 kb genomic resistance island found in a multidrug-resistant Acinetobacter baumannii isolate of European clone I from 1977 ...
Objectives. Multidrug-resistant Acinetobacter strain HK302 was isolated from an outbreak of nosocomial infections in Switzerland in 1977. The aim of the present study was to assess whether this archive strain belongs to one of the known international clonal lineages of Acinetobacter baumannii and whether it harbours a genomic structure related to the AbaR1-like resistance islands.. Methods. Multilocus sequence typing (MLST) and HindIII ribotyping were used to determine the taxonomic position of HK302 at the species and subspecies (clonal) levels. The position and structure of the putative resistance island were investigated by AbaR1-based PCR mapping followed by restriction analysis and partial sequencing of amplicons. A. baumannii AYE harbouring AbaR1 was used as a positive control for PCR mapping.. Results. The MLST allelic profile (1-1-1-1-5-1-1) and HindIII ribotype of HK302 were typical of A. baumannii European (EU) clone I. In addition, an AbaR1-related region inserted into the ATPase gene ...
Welcome to CDC stacks | VIM-2-producing Multidrug-Resistant Pseudomonas aeruginosa ST175 Clone, Spain - 18603 | Emerging...
A total of 183 patients were colonized or infected with multidrug-resistant Pseudomonas aeruginosa isolates at a hospital in Spain during 2007-2010; prevalence increased over this period from 2.8% to 15.3%. To characterize these isolates, we performed molecular epidemiologic and drug resistance analysis. Genotyping showed that 104 (56.8%) isolates belonged to a single major clone (clone B), which was identified by multilocus sequence typing as sequence type (ST) 175. This clone was initially isolated from 5 patients in 2008, and then isolated from 23 patients in 2009 and 76 patients in 2010. PCR analysis of clone B isolates identified the bla(VIM-2) gene in all but 1 isolate, which harbored bla(IMP-22). ST175 isolates were susceptible to only amikacin (75%) and colistin (100%). Emergence of the ST175 clone represents a major health problem because it compromises therapy for treatment of P. aeruginosa nosocomial infections ...
Simple Method for Markerless Gene Deletion in Multidrug-Resistant Acinetobacter baumannii
The traditional markerless gene deletion technique based on overlap extension PCR has been used for generating gene deletions in multidrug-resistant Acinetobacter baumannii. However, the method is time-consuming because it requires restriction digestion of the PCR products in DNA cloning and the con …
Characteristics of multidrug-resistant Acinetobacter baumannii strains isolated in Geneva during colonization or infection |...
Carbapenem resistance in A. baumannii is most often associated with class D β-lactamases (OXA-23-like, OXA-40-like and OXA-58-like) and MBLs. OXA-type carbapenemases are predominant in A. baumannii, particularly in worldwide outbreaks of OXA-23 [24]. The molecular analysis of the isolates tested in this study revealed that 14 strains (51.8 %) carried the blaOXA-23-like gene and that two strains carried a blaOXA-24-like gene. All of the strains had a blaOXA-51-like gene, and four strains had a blaOXA-58 gene. In this study, the OXA-58 isolates presented lower MIC values for meropenem than OXA-23-like-positive isolates, which systematically exhibited higher MIC values (Table 1). The isolates with non-acquired OXA genes displayed a marked variation and included some carbapenem-resistant genes. Naturally occurring OXA carbapenemases, such as OXA-51-like enzymes (e.g., OXA 64-66, OXA 68-71, OXA 78-80, OXA-82, OXA-86, OXA-92 and OXA104-112), have been identified in A. baumannii isolates worldwide. In ...
Draft Genome of Multidrug-Resistant Klebsiella pneumoniae 223/14 Carrying KPC-6, Isolated from a General Hospital in Malaysia ...
Abstract. We performed whole genome sequencing on a clinical multidrug-resistant Klebsiella pneumoniae strain 223/14. Investigation into its draft genome revealed the presence of KPC-6 variant, suggesting carbapenemase is present in this isolate. We found a plasmid-borne KPC gene (882 bp) inserted between two transposase genes in the genome of K. pneumoniae 223/14.. Keywords: Klebsiella pneumoniae Carbapenemase (KPC), multidrug resistance, whole genome sequencing ...
Trends in multidrug-resistant gram-negative bacilli and the role of prolonged β-lactam infusion in the intensive care unit |...
Multidrug-resistant gram-negative bacilli are emerging threats in the intensive care unit setting worldwide. Extended-spectrum β-lactamases, AmpC β-lactamases, and carbapenem-resistant Enterobacteriaceae are increasing at an alarming rate, leaving limited therapeutic options. In addition, multidrug resistance among Pseudomonas aeruginosa and Acinetobacter baumannii has widely disseminated and become a frequent cause of nosocomial infections within many intensive care units. Therefore, resistance is increasing to all currently available antibiotics, including cephalosporins, penicillins, aztreonam, carbapenems, fluoroquinolones, and aminoglycosides. Some multidrug-resistant gram-negative bacteria remain susceptible to only a few antibiotics such as tigecycline, fosfomycin, and polymyxins. The steady trend of increasing resistance coupled with the lack of novel antibiotics targeting resistant gram-negative bacilli has forced clinicians to increasingly apply more aggressive dosing strategies, ...
Table 3 - Multidrug-Resistant Acinetobacter baumannii - Volume 11, Number 1-January 2005 - Emerging Infectious Disease journal ...
To understand the epidemiology of multidrug-resistant (MDR) Acinetobacter baumannii and define individual risk factors for MDR, we used epidemiologic methods, performed organism typing by pulsed-field gel electrophoresis (PFGE), and conducted a matched case-control retrospective study. We investigated 118 patients, on 27 wards, in whom MDR A. baumannii was isolated from clinical cultures. Each case-patient had a control without MDR A. baumannii and was matched for hospital length of stay, ward, and calendar time. The epidemiologic investigation found small clusters of up to 6 patients each with no common identified source. Ten different PFGE clones were found, of which 2 dominated. The PFGE pattern differed within temporospatial clusters, and antimicrobial drug susceptibility patterns varied within and between clones. Multivariate analysis identified the following significant risk factors: male sex, cardiovascular disease, having undergone mechanical ventilation, and having been treated with
Welcome to CDC stacks | Multidrug-Resistant Acinetobacter baumannii Clone, France - 19025 | Emerging Infectious Diseases
In February 2006, a patient colonized with a multidrug-resistant sequence type 56 Acinetobacter baumannii strain was admitted to a hospital in Madrid, Spain. This strain spread rapidly and caused a large outbreak in the hospital. Clinicians should be ...
Genomic definition of hypervirulent and multidrug-resistant Klebsiella pneumoniae clonal groups. - Institut Pasteur
Multidrug-resistant and highly virulent Klebsiella pneumoniae isolates are emerging, but the clonal groups (CGs) corresponding to these high-risk strains have remained imprecisely defined. We aimed to identify K. pneumoniae CGs on the basis of genome-wide sequence variation and to provide a simple bioinformatics tool to extract virulence and resistance gene data from genomic data. We sequenced 48 K. pneumoniae isolates, mostly of serotypes K1 and K2, and compared the genomes with 119 publicly available genomes. A total of 694 highly conserved genes were included in a core-genome multilocus sequence typing scheme, and cluster analysis of the data enabled precise definition of globally distributed hypervirulent and multidrug-resistant CGs. In addition, we created a freely accessible database, BIGSdb-Kp, to enable rapid extraction of medically and epidemiologically relevant information from genomic sequences of K. pneumoniae. Although drug-resistant and virulent K. pneumoniae populations were largely
multidrug-resistant acinetobacter baumannii Archives - PacBio
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Kitchen Cutting Boards Remain a Source of Multidrug-Resistant Bacteria After Use
The spread of multidrug-resistant bacteria has been associated with the hospital setting, but these findings suggest that transmission of drug-resistant E. coli occurs both in the hospital and households, says Andreas Widmer, MD, lead author of the study. Our findings emphasize the importance of hand hygiene, not only after handling raw poultry, but also after contact with cutting boards used in poultry preparation.. Researchers from University Hospital in Basel, Switzerland collected and examined 298 cutting boards (154 from University Hospital and 144 from private households) after preparation of various meats (i.e., poultry, beef/veal, pork, lamb, game and fish) and before being cleaned. They also collected 20 pairs of gloves from hospital kitchen employees after they handled raw poultry. These samples were tested for the presence of ESBL-producing Enterobacteriaceae, a family of gram-negative bacteria that includes Salmonella, E. coli and Klebsiella. In testing the cutting boards, ...
An outbreak of blaOXA-51-like- and blaOXA-66-positive Acinetobacter baumannii ST208 in the emergency intensive care unit |...
A series of clinical isolates of drug-resistant (DR) Acinetobacter baumannii with diverse drug susceptibility was detected from eight patients in the emergency intensive care unit of Tokai University Hospital. The initial isolate was obtained in March 2010 (A. baumannii Tokai strain 1); subsequently, seven isolates were obtained from patients (A. baumannii Tokai strains 2-8) and one isolate was obtained from an air-fluidized bed used by five of the patients during the 3 months from August to November 2011. The isolates were classified into three types of antimicrobial drug resistance patterns (RRR, SRR and SSR) according to their susceptibility (S) or resistance (R) to imipenem, amikacin and ciprofloxacin, respectively. Genotyping of these isolates by multilocus sequence typing revealed one sequence type, ST208, whilst that by a DiversiLab analysis revealed two subtypes. All the isolates were positive for bla
OXA-51-like and bla
OXA-66, as assessed by PCR and DNA sequencing. A. baumannii Tokai strains 1
Common antibiotic azithromycin effectively kills many multidrug-resistant bacteria
Contrary to current medical dogma, researchers at University of California, San Diego School of Medicine and Skaggs School of Pharmacy and Pharmaceutical Sciences report that the common antibiotic azithromycin kills many multidrug-resistant bacteria very effectively - when tested under conditions that closely resemble the human body and its natural antimicrobial factors.
Resistant Pseudomonas aeruginosa Infections Pharmaceutical and Healthcare Pipeline Review H1 | Medgadget
Summary Pharmaceutical and Healthcare disease pipeline guide Resistant Pseudomonas aeruginosa Infections - Pipeline Review, H1 2017, provides an overview o
Resistant Pseudomonas Aeruginosa Infections Drugs Market Insights: Global Industry Analysis, Market Drivers, Restraints,...
Resistant Pseudomonas Aeruginosa Infections Drugs Market Insights: Global Industry Analysis, Market Drivers, Restraints, Opportunities, Applications, Trends And Forecasts 2020-2026
African Journal of Microbiology Research - prevalence of multidrug resistant staphylococcus aureus in gonoshastho nagar...
Antibiotics are used in the prevention of different kinds of infectious diseases, but now-a-days, multidrug resistant strain of pathogenic bacteria is increasing due to the excess or misuse of antibiotics. These antibiotic resistant bacteria have become a great problem for the big population of Bangladesh. In this study, seven clinical samples were screened from about 250 patients having upper respiratory infections at Gonoshasthya Nagar Hospital, Dhanmondi, Dhaka. The isolated S. aureus was screened for their antibiotic resistance profiles. In this study, eleven antimicrobial drugs were used. The objective of this study was to check the sensitivity and resistance ability of S. aureus in different antibiotic concentrations and growth rate at room temperature after isolation, purification and characterization because infectious diseases could play a great role in human health in developing countries due to multidrug resistance activity of bacteria. S. aureus was found to be resistant to eight commonly
Within-host Diversity of Multidrug-Resistant Escherichia coli in the Gut and Bladder
Uropathogenic E. coli are paradoxically able to both cause disease in the urinary tract, and reside there asymptomatically. The pandemic, multi-drug resistant E. coli subclone ST131-H30 (H30) is of special interest, as it has been found to persist in the gut and bladder of healthy people. In order to understand this persistence, we investigated whether H30 is competitive in these niches and thus able to persist by excluding other E. coli, as well as whether H30 may persist via within-host adaptation. In order to assess the E. coli clonal landscape, we developed a novel method based on deep sequencing of two loci, along with an algorithm for analysis of resulting data. Using this method, we assessed fecal and urinary samples from healthy women carrying H30, and found that even in the absence of antibiotic use, H30 could completely dominate the gut and, especially, urine of healthy carriers. In order to ascertain whether H30 adapts within host, we employed population-level whole genome sequencing, ...
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Background: The emergence and rapid spreading of multidrug-resistant Acinetobacter baumannii strains has become a major health threat worldwide. To better understand the genetic recombination related with the acquisition ...
Genomic and Functional Analysis of Emerging Virulent and Multidrug-Resistant Escherichia coli Lineage Sequence Type 648 |...
Notably, ST648 clade 2 strains almost ubiquitously carried an identical ESBL plasmid, as discussed below. Strains from humans and animals with different backgrounds were distributed throughout the phylogeny (Fig. 1A), suggesting ST648s interspecies movement and zoonotic potential while lacking clear genomic signatures that would indicate ecological adaptation to individual host species and infection sites. The geographical distributions were also similar, where strains from different continents occurred throughout the phylogeny (Fig. 1A). These results suggest that ST648 is a host generalist rather than a specialist, with the capability of frequent cross-species transmission, thriving in different clinical and nonclinical contexts, similar to ST131 (8). By including strains from multiple geographic regions and wildlife (Fig. 1A), unique insights into this newly emerging clonal lineage were discovered beyond the usually narrower human, clinically centered focus.. We analyzed the accessory gene ...
CD36 | Selective Inhibitors of Protein Methyltransferases
is an innately multidrug-resistant pathogen which is emerging in cystic fibrosis (CF) patients. species exhibits innate resistance to many antibiotics, including cephalosporins (except ceftazidime), aztreonam, and aminoglycosides (1, 8C10). Clinical strains frequently harbor acquired resistances, especially to ceftazidime, ciprofloxacin, and carbapenems. We have recently described the first resistance-nodulation-cell division (RND)-type multidrug efflux pump in MexAB-OprM efflux pump: AxyABM can extrude cephalosporins (except cefepime), fluoroquinolones, and chloramphenicol. Moreover, AxyABM plays a major role in the innate resistance to aztreonam. Nevertheless, the mechanism(s) leading to aminoglycoside and cefepime resistance remain(s) unknown. It is likely that other efflux systems contribute to the antibiotic resistance of AXX-A strain (GenBank accession number type:entrez-nucleotide,attrs:text:AFRQ01000000″,term_id:339120535″AFRQ01000000). We examined this sequence looking ...
124182-57-6 IC50 | Selective Inhibitors of Protein Methyltransferases
is an innately multidrug-resistant pathogen which is emerging in cystic fibrosis (CF) patients. species exhibits innate resistance to many antibiotics, including cephalosporins (except ceftazidime), aztreonam, and aminoglycosides (1, 8C10). Clinical strains frequently harbor acquired resistances, especially to ceftazidime, ciprofloxacin, and carbapenems. We have recently described the first resistance-nodulation-cell division (RND)-type multidrug efflux pump in MexAB-OprM efflux pump: AxyABM can extrude cephalosporins (except cefepime), fluoroquinolones, and chloramphenicol. Moreover, AxyABM plays a major role in the innate resistance to aztreonam. Nevertheless, the mechanism(s) leading to aminoglycoside and cefepime resistance remain(s) unknown. It is likely that other efflux systems contribute to the antibiotic resistance of AXX-A strain (GenBank accession number type:entrez-nucleotide,attrs:text:AFRQ01000000″,term_id:339120535″AFRQ01000000). We examined this sequence looking ...
Multidrug-resistant organism (MDRO) - St. Jude Childrens Research Hospital
A multidrug-resistant organism is a germ that is resistant to many antibiotics. Find out about prevention and treatment. Get answers to your questions.
Multidrug efflux pump accessory protein elisa and antibody
Shop Multidrug efflux pump accessory protein ELISA Kit, Recombinant Protein and Multidrug efflux pump accessory protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
ESIU alert: pan-resistant bacteria in urinary tract infections | Uroweb
Recently the emergence of pan-resistant bacterial pathogens was published in The Lancet Infectious Disease, online August 11, 2010. In this study 107 enterobacteria isolates from UK, India and Pakistan harbouring a broad spectrum metallo-β- lactamase 1 (called New Delhi metallo-β-lactamase 1; NDM-1) were found to be highly resistant against most antibiotics. Two Klebsiella isolates from the UK have been shown to be resistant against all available antibiotics. These isolates or the plasmids conveying resistance were shown to have been transferred also from country-to-country.. This is the true beginning of a post-antibiotic era, which will have a dramatic impact on our current urological practice. Enterobacteria are the most important species causing urinary tract infections (UTI). Most of the isolates harbouring this NDM-1 enzyme also were from community-acquired UTI.. Whereas in Gram-positive bacteria several new antibiotics are seen on the market, there will be no new antibiotics against ...
Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of...
Serotyping The thirteen isolates belonged to three serotypes: ten strains (77%) to serotype 4b; two strains (15%) to 1/2a, and one strain (8%) to 1/2b (Fig. 3). DISCUSSION Most industrialized countries have developed surveillance systems for listeriosis since 1987 (25), following outbreaks that demonstrated the importance of the foodborne transmission of the disease. Listeriosis has been mainly reported by those countries, with few or even no reports from Africa, Asia and South America. According to Rocourt et al. (25), whether this reflects different consumption patterns, dietary habits, different host susceptibility, or lack of testing facilities is not known. In order to help filling this gap, collecting some data from Brazil was one of the aims of this research. The first antibiotic resistant strain of L. monocytogenes was described in 1988 and, since then, other resistant strains have been detected in food and clinical isolates, including multidrug resistant strains (23). In this study, all ...
IJMS | Free Full-Text | Biochemistry of Bacterial Multidrug Efflux Pumps
Bacterial pathogens that are multi-drug resistant compromise the effectiveness of treatment when they are the causative agents of infectious disease. These multi-drug resistance mechanisms allow bacteria to survive in the presence of clinically useful antimicrobial agents, thus reducing the efficacy of chemotherapy towards infectious disease. Importantly, active multi-drug efflux is a major mechanism for bacterial pathogen drug resistance. Therefore, because of their overwhelming presence in bacterial pathogens, these active multi-drug efflux mechanisms remain a major area of intense study, so that ultimately measures may be discovered to inhibit these active multi-drug efflux pumps.
Cambridge partners with India to fight multidrug resistant TB | University of Cambridge
The Cambridge-Chennai Centre Partnership on Antimicrobial Resistant Tuberculosis will bring together a multidisciplinary team of international researchers, and will be led by Professor Sharon Peacock and Dr Soumya Swaminathan. The team, including Professors Lalita Ramakrishnan, Ken Smith, Tom Blundell and Andres Floto, will focus on developing new diagnostic tools and treatments to address the sharp rise in cases of multidrug resistant tuberculosis (TB). This will include research into:
Expression of the RND-Type Efflux Pump AdeABC in Acinetobacter baumannii Is Regulated by the AdeRS Two-Component System |...
Analysis of adeABC gene expression in CIP 70-10 resistant mutants.Since it is likely that expression of AdeABC in clinical isolate BM4454 is secondary to an alteration in AdeRS, we analyzed this regulatory system in A. baumannii CIP 70-10. This reference strain is susceptible to antibiotics and harbors the adeABC and adeRS genes, as evidenced by PCR (data not shown) and sequencing (GenBank accession no. AY426969). The AdeR regulator from CIP 70-10 shared 98% amino acid identity with that from BM4454, and the AdeS sensor shared 96.7% amino acid identity with that from BM4454, although the latter protein was shorter by 4 amino acids. The proteins contained the conserved motifs previously reported for histidine kinases and response regulators (32), and inside these motifs, no differences were detected between the sequences from the two strains. Spontaneous one-step mutants of CIP 70-10 exhibiting a multidrug resistance phenotype indistinguishable from that of BM4454 were selected on BHI agar ...
The diversity of definitions of multidrug-resistant (MDR) and pandrug-resistant (PDR) Acinetobacter baumannii and Pseudomonas...
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Pseudomonas Aeruginosa AmpR: An Acute-Chronic Switch Regulator - PubMed
Pseudomonas aeruginosa is one of the most intractable human pathogens that pose serious clinical challenge due to extensive prevalence of multidrug-resistant clinical isolates. Armed with abundant virulence and antibiotic resistance mechanisms, it is a major etiologic agent in a number of acute and …
Old antibiotics for emerging multidrug-resistant bacteria. - PubMed - NCBI
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
UM Research Repository
Parasakthi, N.; Vadivelu, J.; Ariffin, H.; Iyer, L.; Palasubramaniam, Selvi; Arasu, A. (2000) Epidemiology and molecular characterization of nosocomially transmitted multidrug-resistant Klebsiella pneumoniae. International Journal of Infectious Diseases, 4 (3). pp. 123-128. ISSN 12019712. Full text not available from this repository ...
ARDB-Antibiotic Resistance Genes Database
1) Magnet S, et al. (2001) Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454.. Antimicrob Agents Chemother 45(12):3375-80 PubMed: 11709311 ...
ARDB-Antibiotic Resistance Genes Database
1) Magnet S, et al. (2001) Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454.. Antimicrob Agents Chemother 45(12):3375-80 PubMed: 11709311 ...
HCV Research and News: 07/21/10
The authors note that as and when ART becomes universally available to drug users with HIV, and their health status improves, so their other health problems will take on increased prominence, such as non-AIDS related comorbitities and TB, all of which will come with their own treatment priorities. HIV infected drug users with TB co-infection creates various clinical challenges since TB can be difficult to diagnose in HIV patients due to atypical chest radiographs, high-rates of TB in parts of the body outside the usual setting of the lungs, and the reduced sensitivity of skin tests used to diagnose TB in HIV patients. While people with latent TB but not HIV infection have a roughly 1 in 11 lifetime risk of having their TB develop into full blown disease, it becomes a 1 in 11 annual risk in patients with HIV co-infection. Concentration of people with HIV and substance use disorders behind bars facilitates transmission of TB, including multidrug resistant strains, due to overcrowding and increased ...
RE: multi drug resistance protein
Good Morning David, Ive done several years of Multi Drug Resistance Protein work. It was a tough nut to crack back a few years ago. We use JSB-1 primary antibody [far superior to C219] and the procedure is long... weve added extra IgG fragments to give the DAB some extra places to bind. Its really a nice, clean, amplified method. I can send you the worksheet for the procedure if youd like. Please find the related publications below (methods paper is #2). I think #3 may be of special interest to you. 1)Toth, K., Vaughan, M.M., Slocum, H.K., Fredericks, W.J., Chen, Y., Arredondo, M.A., Harstrick, A., Karakousis, C., Baker, R.M., Rustum, Y.M. Comparison of an Immunoperoxidase sandwich Staining Method and Western Blot Detection of P-glycoprotein in Human Cell Lines and Sarcomas. Amer. J. Path. 140:1009-1016, 1992. 2)Toth, K., Vaughan, M.M., Slocum, H.K., Arredondo, M.A., Takita, H., Baker, R.M., Tsuro, T., and Rustum, Y.M. New Immunohistochemical Sandwich Staining Method for MDR1 ...
Introduction - Multi Drug Resistance: A Global Concern
Multiple drug resistance among bacteria has become a global issue with a considerable impact on the mortality associated with infectious diseases. This book is a detailed compilation of available knowledge on the surveillance and mechanisms of antibiotic resistance in various countries throughout the world. Readers will be updated on current information on the understanding of mechanisms involved in drug resistance and the geographical distribution of resistance determinant markers. This volume should be a useful guide for microbiologists and clinicians interested in designing antimicrobial therapies tailored for patients in specific geographical regions ...
The role of artificial intelligence in the battle against antimicrobial-resistant bacteria | SpringerLink
Rapid-Sequencing the Superbug | Medgadget
A recent investigative report by Steve Silberman from Wired about the highly resistant Acinetobacter baumannii, encountered by clinicians treating wounded
Prevelence of Multi Drug Resistant S. aureus in Rayalaseema Region, Andra Pradesh, South India| Abstract
To study the prevalence and antibiotic resistance patterns ofMDR S. aureus frompyogenic infections in diabetic and non-diabetic patients fromdifferent..
News & Events | European Centre for Disease Prevention and Control
Antimicrobial resistance represent a serious threat to public health and patient safety and is a worldwide problem. Each year, in the European Union (EU) at least 25 000 patients die of infections with multidrug-resistant bacteria. ...
Facts About Multi-Drug Resistant Organisms | Way to Grow | CHKD
MDROs are everywhere in the world. Hospitals and other healthcare settings battle resistant organisms to keep them from spreading throughout their buildings. MDROs are found in the community as well. Examples of infections from MDROs in the community are drug resistant pneumonias and skin and soft tissue infections.
Molecular Mechanisms of Antibiotic Resistance - Part II
All antibiotic resistance mechanisms in bacteria ultimately work by preventing, or mitigating the effects of, an antibacterial inhibitor binding to it
Photoactivated QDs Kill Antibiotic-Resistant Superbugs | BioScan | Apr 2016 | BioPhotonics
A technique for treating bacterial infections has successfully used light-activated quantum dots (QDs) to kill multiple multidrug-resistant strains.
IPPE: What is Risk from Multi-Drug Resistant Antibiotics? | The Poultry Site
Dr Hurd added that all scientific risk assessments have shown that there is negligible risk from antibiotic use on the farm. He said very few people are going to be treated with the antibiotic that are used on the farm when they are treated for a food poisoning event and most doctors will not prescribe antibiotics for food poising in the first place ...
Releases of Multi-drug Resistant Bacteria to the Enviorment
chilling reality is that EPA states in its sludge rule that exposure through the air, water, or food to any of the pollutants (e ...