Drosophila willistoni (Sturtevant, 1916) é uma espécie do grupo willistoni de Drosophila que apresenta ampla distribuição geográfica desde o sul dos Estados Unidos (Flórida) e México até o norte da Argentina. Esta espécie tem sido alvo de muitos estudos evolutivos dentro do grupo devido à sua considerável capacidade de explorar de forma bem sucedida diversos tipos de ambientes e também por sua grande variabilidade genética expressa através de diferentes marcadores. A linhagem 17A2 de D. willistoni foi coletada em 1991 no Estado do Rio Grande do Sul, Brasil (30°05S, 51°39W), e vem sendo mantida desde então no laboratório de Drosophila da Universidade Federal do Rio Grande do Sul. Diferentemente das demais linhagens de D. willistoni mantidas no laboratório, a linhagem 17A2 já produziu espontaneamente machos do tipo white (olhos brancos) e do tipo sepia (olhos marrons) em estoques mantidos a 170C. A fim de avaliar se esta linhagem é potencialmente hipermutável, nós ...
Events - 56th Annual Drosophila Research Conference - Genetics Society Of America - Sheraton Chicago Hotel - Chicago - Illinois - - - Event Overview: \r\n The Annual Drosophila Research Conference is the preeminent meeting for Drosophila researchers. From students and trainees to PIs and Faculty, there is something for everyone. Nearly 1,000 abstracts are submitted each year and over 150 are selected for platform presentations ranging from model organisms to human disease to cell biology, pattern formation and more. In addition there are over a dozen plenary sessions that are varied in scope. Trainees can also benefit from the experience as there are several sessions that can help them with their career search, presentation skills, grant writing, and much more. Workshops on other topics of interest are also held in conjunction with the conference. The Annual Drosophila Research Conference is an opportunity to learn about the latest research, network with colleagues and share your scientific
There are thousands of different species of flies. Larvae feed not on the vegetable matter itself, but on the yeasts and microorganisms present on the decaying breeding substrate. All that is needed for development is a moist film of fermenting material. The Hawaiian species of Drosophila (estimated to be more than 500, with roughly 380 species described) are sometimes recognized as a separate genus or subgenus, Idiomyia,[3][47] but this is not widely accepted. About 250 species are part of the genus Scaptomyza, which arose from the Hawaiian Drosophila and later recolonized continental areas. They are particularly fond of bananas among fruits. [24], The following section is based on the following Drosophila species: Drosophila serrata, Drosophila pseudoobscura, Drosophila melanogaster, and Drosophila neotestacea. [9] A few species have switched to being parasites or predators. The term Drosophila, meaning dew-loving, is a modern scientific Latin adaptation from Greek words δρόσος, ...
TY - JOUR. T1 - Transcription control of a gene for Drosophila transcription factor, DREF by DRE and cis-elements conserved between Drosophila melanogaster and virilis. AU - Kwon, Eunjeong. AU - Seto, Hirokazu. AU - Hirose, Fumiko. AU - Ohshima, Nobuko. AU - Takahashi, Yasuhiko. AU - Nishida, Yasuyoshi. AU - Yamaguchi, Masamitsu. PY - 2003/5/8. Y1 - 2003/5/8. N2 - A DNA replication-related element (DRE)-binding factor (DREF) has been revealed to be an important transcription factor for activating promoters of cell proliferation and differentiation related genes. The amino acid sequences of DREF are conserved in evolutionary separate Drosophila species, Drosophila melanogaster (Dm) and Drosophila virilis (Dv) in three regions. In the present study, evidence was obtained that there are several highly conserved regions in the 5′ flanking region between the DmDREF and DvDREF genes. Band mobility shift assays using oligonucleotides corresponding to these conserved regions revealed that specific ...
Flybase THE Database of Drosophila Genes & Genomes. NCBI Pubmed citations from the biomedical literature. Flymove images and movies of Drosophila development. Atlas of Drosophila development illustrations of embryogenesis. Drosophila protocols a list of lab homepages with Drosophila protocols JEDI network of junior Drosophila investigators. BSDB the British society of Developmental Biology US fly meeting the American annual Drosophila research conference. European fly meeting the European biannual Drosophila research conference. ...
TY - JOUR. T1 - Characterizing recurrent positive selection at fast-evolving genes in Drosophila miranda and Drosophila pseudoobscura. AU - Jensen, Jeffrey. AU - Bachtrog, Doris. PY - 2010. Y1 - 2010. N2 - Characterizing the distribution of selection coefficients in natural populations remains a central challenge in evolutionary biology. We resequenced a subset of 19 fast-evolving protein-coding genes in the sister species Drosophila miranda and D. pseudoobscura and their flanking regions to characterize the spatial footprint left by recurrent and recent selection. Consistent with previous findings, fast-evolving genes and their flanking regions show reduced levels of neutral diversity compared with randomly chosen genes, as expected under recurrent selection models. Applying a variety of statistical tests designed for the detection of selection at different evolutionary timescales, we attempt to characterize parameters of adaptive evolution. In D. miranda, fast-evolving genes generally show ...
DNA packaging affects a genes availability for transcription. Typically, loosely-packed euchromatic regions are gene rich and exhibit higher levels of gene transcription, while densely-packed heterochromatic regions are gene poor and exhibit less gene transcription. The 4th chromosome, or F Element, of Drosophila is unique, as it contains ~80 genes and is heterochromatic in nature, yet these genes are actively transcribed. Thus, Drosophila species provide an ideal model system for exploring how genes are accessed in heterochromatic regions and how well these mechanisms are conserved over millions of years of evolution. Working alongside the Genomics Education Partnership (GEP), genes of the F Element and the control D Element are being annotated across Drosophila species to identify unique features of genes on the F Element. My work has focused on contig1 of Drosophila ananassae. We have annotated the coding spans (CDS) of contig1, which contains five genes located on the autosomal euchromatic 3L
Cactophilic Drosophila species provide a valuable model to study gene-environment interactions and ecological adaptation. Drosophila buzzatii and Drosophila mojavensis are two cactophilic species that belong to the repleta group, but have very different geographical distributions and primary host plants. To investigate the genomic basis of ecological adaptation, we sequenced the genome and developmental transcriptome of D. buzzatii and compared its gene content with that of D. mojavensis and two other noncactophilic Drosophila species in the same subgenus. The newly sequenced D. buzzatii genome (161.5 Mb) comprises 826 scaffolds (|3 kb) and contains 13,657 annotated protein-coding genes. Using RNA sequencing data of five life-stages we found expression of 15,026 genes, 80% protein-coding genes, and 20% noncoding RNA genes. In total, we detected 1,294 genes putatively under positive selection. Interestingly, among genes under positive selection in the D. mojavensis lineage, there is an excess of genes
At the Genetics Society of America 53rd Annual Drosophila Research Conference in Chicago, shared several scientific research their knowledge about some of these diseases, including ataxia-telangiectasia , a neurodegenerative disorder, Rett syndrome, a neurological disorder, and kidney stones, a common health condition. All are the subject of ongoing research by the Drosophila model system.. Studying Drosophila Advances researching human diseasesMore than two thirds of the human genes colleagues in the well-studied fruit fly, Drosophila melanogaster, so although it may seem that people do not have much in common with flies, the correspondence of our genetic instructions is astonishing. In fact, there are hundreds of hereditary diseases in humans, the Drosophila colleagues.I do not know holding Lecture in Northern IrelandThe Northern Ireland office to the British Psychological Society and the Queens University of Belfast are happy six eminent psychologist known to throughout Ireland and Britain, ...
Previous experiments have shown two germline stem cell genes, bam and bgcn, to be under strong positive selection in Drosophila melanogaster and Drosophila simulans (Bauer DuMont et al. 2007). This prompted the question of whether the same pattern of selection observed in these two species was present in the germline stem cell genes of other Drosophila lineages? The Aquadro Lab has been sequencing many germline stem cell genes in Drosophila species, and the answer to this question so far has been that some lineages show strong positive selection and some do not. This observation led the Aquadro Lab to begin to test hypotheses about the driver - or drivers - of the positive selection in the germline stem cell genes across some Drosophila lineages. One hypothesis proposed by Bauer DuMont et al. (2007) is that coevolution with pathogens such as the reproductive parasite, Wolbachia pipientis, infecting the germline could be driving this observed selection. This project looked for signs of selection ...
TY - JOUR. T1 - Further studies on gene polymorphism in the mainbody and geographically isolated populations of Drosophila pseudoobscura. AU - Prakash, S.. PY - 1977/12/1. Y1 - 1977/12/1. N2 - The authors have examined polymorphism at 22 additional loci in the populations from the mainbody of Drosophila pseudoobscura and an isolated population from Bogota, Colombia, which also shows partial reproductive isolation from mainbody populations. These studies extend previous observations of reduced gene polymorphism and apparent lack of unique allele in the Bogota population.. AB - The authors have examined polymorphism at 22 additional loci in the populations from the mainbody of Drosophila pseudoobscura and an isolated population from Bogota, Colombia, which also shows partial reproductive isolation from mainbody populations. These studies extend previous observations of reduced gene polymorphism and apparent lack of unique allele in the Bogota population.. UR - ...
We have analyzed the sterility associated with introgressions of the distal one-fourth of the X chromosome from either Drosophila mauritiana or Drosophila sechellia into the genome of Drosophila simulans using a series of visible and DNA markers. Because in Drosophila hybrids, male sterility is usually complete and is often tightly linked with each of several markers used in crosses, a simple genetic basis has generally been assumed. In our low resolution mapping experiment, we were not able to reject the null hypothesis that a single gene, introgressed from either D. mauritiana or D. sechellia, is the cause of male sterility. High resolution mapping, however, reveals a much more complex picture. At least three distinct factors from D. mauritiana, or two from D. sechellia, were identified that need to be jointly present to confer full sterility. Each individual factor by itself is relatively ineffective in causing sterility, or even a partial spermatogenic defect. Moreover, there appear to be ...
Other articles where Drosophila serrata is discussed: evolution: Ethological (behavioral) isolation: The vinegar flies Drosophila serrata, D. birchii, and D. dominicana are three sibling species (that is, species nearly indistinguishable morphologically) that are endemic in Australia and on the islands of New Guinea and New Britain. In many areas these three species occupy the same territory, but no hybrids…
Many introns in Drosophila and other invertebrates are less than 80 nucleotides in length, too small to be recognized by the vertebrate splicing machinery. Comparison of nuclear splicing extracts from human HeLa and Drosophila Kc cells has revealed species-specificity, consistent with the observed size differences. Here we present additional results with the 68 nucleotide fifth intron of the Drosophila myosin heavy chain gene. As observed with the 74 nucleotide second intron of the Drosophila white gene, the wild-type myosin intron is accurately spliced in a homologous extract, and increasing the size by 16 nucleotides both eliminates splicing in the Drosophila extract and allows accurate splicing in the human extract. In contrast to previous results, however, an upstream cryptic 5 splice site is activated when the wild-type myosin intron is tested in a human HeLa cell nuclear extract, resulting in the removal of a 98 nucleotide intron. The size dependence of splicing in Drosophila extracts is ...
Drosophila buzzatii has been found sympatric in Argentina with a closely-related sibling species, D. serido. The biogeographical, reproductive and chromosomal data allow us to combine these species into an evolutionary unit, the buzzatii cluster. Salivary gland chromosomes also have been used to determine their phylogenetic relationships with other closely related species, showing that the buzzatii cluster species share two inversions-2d2 and 2s6-with the species of the martensis cluster. Both clusters arose from South American populations of the ancestor of the mulleri complex, and we propose to include D. buzzatii and D. serido in the mulleri complex of the repleta group.. ...
TY - JOUR. T1 - Higher rates of nucleotide substitution in Drosophila than in mammals. AU - Moriyama, Etsuko N.. PY - 1987/1/1. Y1 - 1987/1/1. N2 - To examine whether the rate of nucleotide substitution is affected by generation time of the organism, I attempted to estimate an accurate rate of synonymous (silent) substitution in Drosophila lineages, using alcohol dehydrogenase (Adh) and heat shock protein 82 (hsp82) genes. The results obtained suggest that the rate of synonymous substitution in Drosophila lineages is roughly 10-8 per site per year. This rate is approximately two times higher than that of rodents and ten times greater than higher primates. The higher rate in Drosophila may be explained by the shorter generation times of the Drosophila species, though the possibility that the mutation mechanism in Drosophila may differ from that in mammals cannot be excluded.. AB - To examine whether the rate of nucleotide substitution is affected by generation time of the organism, I attempted to ...
The standard view of adaptation to larval crowding in fruitflies, built on results from 25 years of multiple experimental evolution studies on Drosophila melanogaster, was that enhanced competitive ability evolves primarily through increased larval feeding and foraging rate, and increased larval tolerance to nitrogenous wastes, at the cost of efficiency of food conversion to biomass. These results were at odds from the predictions of classical K-selection theory, notably the expectation that selection at high density should result in the increase of efficiency of conversion of food to biomass, and were better interpreted through the lens of α-selection. We show here that populations of D. ananassae and D. n. nasuta subjected to extreme larval crowding evolve greater competitive ability and pre-adult survivorship at high density, primarily through a combination of reduced larval duration, faster attainment of minimum critical size for pupation, greater time efficiency of food conversion to ...
Kitagawa M., Oyama T., Kawashima T., Yedvobnick B., Kumar A., Matsuno K., Harigaya K.. Mastermind (Mam) has been implicated as an important positive regulator of the Notch signaling pathway by genetic studies using Drosophila melanogaster. Here we describe a biochemical mechanism of action of Mam within the Notch signaling pathway. Expression of a human sequence related to Drosophila Mam (hMam-1) in mammalian cells augments induction of Hairy Enhancer of split (HES) promoters by Notch signaling. hMam-1 stabilizes and participates in the DNA binding complex of the intracellular domain of human Notch1 and a CSL protein. Truncated versions of hMam-1 that can maintain an association with the complex behave in a dominant negative fashion and depress transactivation. Furthermore, Drosophila Mam forms a similar complex with the intracellular domain of Drosophila Notch and Drosophila CSL protein during activation of Enhancer of split, the Drosophila counterpart of HES. These results indicate that Mam is ...
In Drosophila ananassae, artificial selection was carried out for fast and slow remating speed for 10 generations. Response to selection resulted in rapid divergence in remating time in each of two replicates of both fast and slow lines. There were significant differences in mean remat-ing time in females among fast, slow, and control lines. Regression coefficients for both fast and slow lines are significantly different from zero. The realized heritability over 10 genera-tions of selection is from 0.26 to 0.33 for two replicates of fast line and from 0.23 to 0.27 for two replicates of slow line. These findings suggest that female remating time in D. ananassae is under polygenic control. Remating frequency of females showed a correlated response in both fast and slow lines. At generation 10, correlated response to selection was also investigated. Mating propensity of D. ananassae of fast and slow lines was observed in an Elens-Wattiaux mating chamber. Fifteen pairs per test showed that on the ...
Many new Drosophila genomes have been sequenced in recent years using new-generation sequencing platforms and assembly methods. Transposable elements (TEs), being repetitive sequences, are often misassembled, especially in the genomes sequenced with short reads. Consequently, the mobile fraction of many of the new genomes has not been analyzed in detail or compared with that of other genomes sequenced with different methods, which could shed light into the understanding of genome and TE evolution. Here we compare the TE content of three genomes: D. buzzatii st-1, j-19, and D. mojavensis. We have sequenced a new D. buzzatii genome (j-19) that complements the D. buzzatii reference genome (st-1) already published, and compared their TE contents with that of D. mojavensis. We found an underestimation of TE sequences in Drosophila genus NGS-genomes when compared to Sanger-genomes. To be able to compare genomes sequenced with different technologies, we developed a coverage-based method and applied it to the D
The Annual Drosophila Research Conference is the premier meeting for Drosophila researchers. As many as 1,000 presentations cover the full diversity of Drosophila investigations, from genetics to molecular biology, cell biology, development, immunology, physiology, neuroscience, evolution, and more.. ...
Hi Marc, I am working a little bit with drosophila epithelium, specifically the abdomen. What I can say is that it is not very difficult to dissect Drosophila pupae (at leats with 26h APF). There is a protocol in the web from a Nicolas Gompel that is very good. Good Luck Pedro Marco Antunes wrote: , , Hello! , Im interested in working in the Drosophila pupal epithelium. , However, most literature about Drosophila pupa is very old... , Does anyone have some ideas about the difficulties and protocols for , dissecting and manipulating Drosophila pupae (without getting it killed)? , Also, does anyone know which is the best part of the pupa to visualize the , epithelium? The Thorax or the Abdomen? , Thank you for any help! , Marc , _______________________________________________ , Dros mailing list , Dros from net.bio.net , http://www.bio.net/biomail/listinfo/dros , , -- View this message in context: http://www.nabble.com/Drosophila-pupal-epithelium-tf2941767.html#a9310296 Sent from the Bio.net - ...
Accurate models of gene structure including untranslated regions (UTRs), intron-exon boundaries, as well as coding sequences are essential for proper interpretation of molecular genetics (Fire et al. 1998, Jinek et al. 2012), demographic inference (Halligan and Keightley 2006, Parsch et al. 2010, Clemente and Vogl 2012), tests of selection (Mcdonald and Kreitman 1991), and comparative genomics (Chen et al. 2014). The Drosophila offer an excellent model for comparative genomics, with high-quality sequenced genomes for 12 species(Drosophila Twelve Genomes Consortium 2007) as well as draft genomes for an additional eight species (Chen et al. 2014) spanning a total of 63 million years (Tamura et al. 2004). Previous gene models provided for the 12 Drosophila genomes focused on gene prediction with the aid of homology to establish putative annotations of coding sequences across taxa with 15,000−16,000 genes for most species (Drosophila Twelve Genomes Consortium 2007). These gene models produce ...
Species of the Drosophila obscura species group (e.g., D. pseudoobscura, D. subobscura) have served as favorable models in evolutionary studies since the 1930s. Despite numbers of studies conducted with varied types of data, the basal phylogeny in this group is still controversial, presumably owing to not only the hypothetical rapid radiation history of this group, but also limited taxon sampling from the Old World (esp. the Oriental and Afrotropical regions). Here we reconstruct the phylogeny of this group by using sequence data from 6 loci of 21 species (including 16 Old World ones) covering all the 6 subgroups of this group, estimate the divergence times among lineages, and statistically test the rapid radiation hypothesis. Phylogenetic analyses indicate that each of the subobscura, sinobscura, affinis, and pseudoobscura subgroups is monophyletic. The subobscura and microlabis subgroups form the basal clade in the obscura group. Partial species of the obscura subgroup (the D. ambigua/D. obscura
Species of the Drosophila obscura species group (e.g., D. pseudoobscura, D. subobscura) have served as favorable models in evolutionary studies since the 1930s. Despite numbers of studies conducted with varied types of data, the basal phylogeny in this group is still controversial, presumably owing to not only the hypothetical rapid radiation history of this group, but also limited taxon sampling from the Old World (esp. the Oriental and Afrotropical regions). Here we reconstruct the phylogeny of this group by using sequence data from 6 loci of 21 species (including 16 Old World ones) covering all the 6 subgroups of this group, estimate the divergence times among lineages, and statistically test the rapid radiation hypothesis. Phylogenetic analyses indicate that each of the subobscura, sinobscura, affinis, and pseudoobscura subgroups is monophyletic. The subobscura and microlabis subgroups form the basal clade in the obscura group. Partial species of the obscura subgroup (the D. ambigua/D. obscura
Domain architectures containing the following SCOP superfamilies _gap_,57625,57424,88713,_gap_ in Drosophila ananassae 1.3. Domain architectures illustrate each occurrence of _gap_,57625,57424,88713,_gap_.
Königer, Annabella (2019): The molecular basis of cold tolerance in Drosophila ananassae. Dissertation, LMU München: Faculty of Biology ...
In Drosophila embryos, checkpoints maintain genome stability by delaying cell cycle progression that allows time for damage repair or to complete DNA synthesis. Drosophila MOF, a member of MYST histone acetyl transferase is an essential component of male X hyperactivation process. Until recently its involvement in G2/M cell cycle arrest and defects in ionizing radiation induced DNA damage pathways was not well established. Drosophila MOF is highly expressed during early embryogenesis. In the present study we show that haplo-insufficiency of maternal MOF leads to spontaneous mitotic defects like mitotic asynchrony, mitotic catastrophe and chromatid bridges in the syncytial embryos. Such abnormal nuclei are eliminated and digested in the yolk tissues by nuclear fall out mechanism. MOF negatively regulates Drosophila checkpoint kinase 2 tumor suppressor homologue. In response to DNA damage the checkpoint gene Chk2 (Drosophila mnk) is activated in the mof mutants, there by causing centrosomal inactivation
TY - JOUR. T1 - Molecular and cytological analysis of widely-used Gal4 driver lines for Drosophila neurobiology. AU - Ogienko, Anna A.. AU - Andreyeva, Evgeniya N.. AU - Omelina, Evgeniya S.. AU - Oshchepkova, Anastasiya L.. AU - Pindyurin, Alexey V.. PY - 2020/10/1. Y1 - 2020/10/1. N2 - Background: The Drosophila central nervous system (CNS) is a convenient model system for the study of the molecular mechanisms of conserved neurobiological processes. The manipulation of gene activity in specific cell types and subtypes of the Drosophila CNS is frequently achieved by employing the binary Gal4/UAS system. However, many Gal4 driver lines available from the Bloomington Drosophila Stock Center (BDSC) and commonly used in Drosophila neurobiology are still not well characterized. Among these are three lines with Gal4 driven by the elav promoter (BDSC #8760, #8765, and #458), one line with Gal4 driven by the repo promoter (BDSC #7415), and the 69B-Gal4 line (BDSC #1774). For most of these lines, the ...
Two recent studies have presented conflicting views on variation present within the 294 base third domain of the 12S rRNA gene in the genus Drosophila, and in D. pseudoobscura in particular. One study suggested that this gene is highly invariant across the genus, while another recovered 22 distinct haplotypes from 22 strains of D. pseudoobscura. We have sequenced this gene in numerous lines of D. pseudoobscura and its relatives, noting only two haplotypes in the third domain, and we failed to confirm any of the published sequences. Second, we note that the published sequence divergence between strains of D. pseudoobscura was as great as that documented between distantly related Drosophila species. Third, we show that the published polymorphisms of this region within D. pseudoobscura would disrupt the secondary structure of the resulting molecule. We conclude that the published 12S rRNA sequences of D. pseudoobscura do not accurately reflect variability of the functional gene, and that this gene is
TY - JOUR. T1 - The actin-binding protein Lasp promotes Oskar accumulation at the posterior pole of the Drosophila embryo. AU - Suyama, Ritsuko. AU - Jenny, Andreas. AU - Curado, Silvia. AU - Pellis-van Berkel, Wendy. AU - Ephrussi, Anne. PY - 2009/4/14. Y1 - 2009/4/14. N2 - During Drosophila oogenesis, oskar mRNA is transported to the posterior pole of the oocyte, where it is locally translated and induces germ-plasm assembly. Oskar protein recruits all of the components necessary for the establishment of posterior embryonic structures and of the germline. Tight localization of Oskar is essential, as its ectopic expression causes severe patterning defects. Here, we show that the Drosophila homolog of mammalian Lasp1 protein, an actin-binding protein previously implicated in cell migration in vertebrate cell culture, contributes to the accumulation of Oskar protein at the posterior pole of the embryo. The reduced number of primordial germ cells in embryos derived from lasp mutant females can be ...
Cyclin Y is a highly conserved member of the Cyclin superfamily of proteins. In Drosophila the Cyclin Y gene (CycY) is required for progression through several stages of development but the specific pathways that Cyclin Y belongs to and that account for its requirement are not known. Studies in human and Drosophila cell lines have shown that membrane-localized Cyclin Y is required for phosphorylation of the wingless/Wnt co-receptor, arrow/LRP6, and for full activation of the canonical wingless/Wnt pathway. CycY null Drosophila, however, do not phenocopy loss-of-function mutations in canonical wingless pathway genes, suggesting that Cyclin Y may have additional roles outside the wingless pathway in vivo. To identify roles for Cyclin Y in Drosophila I used RNAi to knock down CycY expression in 31 distinct tissue patterns. The screen revealed that expression of the CycY shRNA in specific tissue patterns causes larval lethality and other developmental defects. Knockdown of CycY but not arrow in imaginal
Circularization was recently recognized to broadly expand transcriptome complexity. Here, we exploit massive Drosophila total RNA-sequencing data, |5 billion paired-end reads from |100 libraries covering diverse developmental stages, tissues, and cultured cells, to rigorously annotate |2,500 fruit fly circular RNAs. These mostly derive from back-splicing of protein-coding genes and lack poly(A) tails, and the circularization of hundreds of genes is conserved across multiple Drosophila species. We elucidate structural and sequence properties of Drosophila circular RNAs, which exhibit commonalities and distinctions from mammalian circles. Notably, Drosophila circular RNAs harbor |1,000 well-conserved canonical miRNA seed matches, especially within coding regions, and coding conserved miRNA sites reside preferentially within circularized exons. Finally, we analyze the developmental and tissue specificity of circular RNAs and note their preferred derivation from neural genes and enhanced accumulation in
A single species of fly, Drosophila melanogaster, has been the subject of scientific research for more than one hundred years. Why does this tiny insect merit such intense scrutiny?. Drosophilas importance as a research organism began with its short life cycle, ability to reproduce in large numbers, and easy-to-see mutant phenotypes. Over time, laboratory investigation revealed surprising similarities between flies and other animals at the level of genes, gene networks, cell interactions, physiology, immunity, and behavior. Like humans, flies learn and remember, fight microbial infection, and slow down as they age. Scientists use Drosophila to investigate complex biological activities in a simple but intact living system. Fly research provides answers to some of the most challenging questions in biology and biomedicine, including how cells transmit signals and form ordered structures, how we can interpret the wealth of human genome data now available, and how we can develop effective treatments ...
Metz, Charles William, Moses, Mildred S., Mason, Eleanor D. (July 1923) Genetic studies on Drosophila virilis with considerations on the genetics of other species of Drosophila. Carnegie Institution of Washington Publication No. 328 . Carnegie Institution of Washington , Washington, D.C., pp. 1-94. ...
Vectors derived from the Drosophila P element transposon are widely used to make transgenic Drosophila. Insertion of most P-element-derived vectors is nonrandom, but they exhibit a broad specificity of target sites. During experiments to identify cis-acting regulatory elements of the Drosophila segmentation gene engrailed, we identified a fragment of engrailed DNA that, when included within a P-element vector, strikingly alters the specificity of target sites. P-element vectors that contain this fragment of engrailed regulatory DNA insert at a high frequency near genes expressed in stripes.. ...
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N6-methyladenosine (m6A) is the most common internal modification of eukaryotic messenger RNA (mRNA) and is decoded by YTH domain proteins1, 2, 3, 4, 5, 6, 7. The mammalian mRNA m6A methylosome is a complex of nuclear proteins that includes METTL3 (methyltransferase-like 3), METTL14, WTAP (Wilms tumour 1-associated protein) and KIAA1429. Drosophila has corresponding homologues named Ime4 and KAR4 (Inducer of meiosis 4 and Karyogamy protein 4), and Female-lethal (2)d (Fl(2)d) and Virilizer (Vir)8, 9, 10, 11, 12. In Drosophila, fl(2)d and vir are required for sex-dependent regulation of alternative splicing of the sex determination factor Sex lethal (Sxl)13. However, the functions of m6A in introns in the regulation of alternative splicing remain uncertain3. Here we show that m6A is absent in the mRNA of Drosophila lacking Ime4. In contrast to mouse and plant knockout models5, 7, 14, Drosophila Ime4-null mutants remain viable, though flightless, and show a sex bias towards maleness. This is ...
The circulatory system of Drosophila melanogaster represents an easily amenable genetic model whose analysis at different levels, i.e., from single molecules up to functional anatomy, has provided new insights into general aspects of cardiogenesis, heart physiology and cardiac aging, to name a few examples. In recent years, the Drosophila heart has also attracted the attention of researchers in the field of biomedicine. This development is mainly due to the fact that several genes causing human heart disease are also present in Drosophila, where they play the same or similar roles in heart development, maintenance or physiology as their respective counterparts in humans. This review will attempt to briefly introduce the anatomy of the Drosophila circulatory system and then focus on the different cell types and non-cellular tissue that constitute the heart.
The generation of cell polarity through the localization of specific mRNAs and proteins to discrete subcellular sites is fundamental to asymmetric cell division, tissue morphogenesis, cell migration, and most other developmental processes. While many different localized mRNAs and proteins have been described, the mechanisms by which such molecules become localized are only poorly understood. In the first part of this dissertation, I describe my efforts to unravel the mechanism by which gurken (grk) mRNA becomes localized to the anterodorsal corner of the Drosophila oocyte during mid-oogenesis. Such localization is a key step in the polarization of the mature Drosophila egg and future embryo; defects in grk mRNA localization result in the production of depolarized eggs that give rise to embryos that fail to specify ectodermal, endodermal and mesodermal germ layers and die before hatching. I show, using a transgenic fly assay system, that a conserved sequence element within the grk mRNA, called ...
P elements containing a 7 kb DNA fragment from the middle of the Drosophila bithorax complex insert preferentially into the bithorax complex or into the adjacent chromosome regions. This homing property is similar to that reported for the engrailed promoter (Hama, C., Ali, Z. and Kornberg, T. B. (1990) Genes Dev. 4, 1079-1093). The 7 kb fragment does not contain any known promoter, but it acts as a boundary element separating adjacent segmental domains. An enhancer-trap P element was constructed with the homing fragment and the selectable marker flanked by FRT sites. P insertions can be trimmed down by Flp-mediated recombination to just the lacZ reporter, so that the (beta)-galactosidase pattern is not influenced by sequences inside the P element. Twenty insertions into the bithorax complex express (beta)-galactosidase in segmentally limited patterns, reflecting the segmental domains of the bithorax complex where the elements reside. The mapping of segmental domains has now been revised, with ...
The chordotonal (Ch) organ, an internal stretch receptor located in the subepidermal layer, is one of the major sensory organs in the peripheral nervous system of Drosophila melanogaster. Although the cell lineage of the Ch organ has been well characterized in many studies, the determination machinery of Ch organ precursor cells (COPs) remains largely unresolved. Here we report that the rhomboid (rho) gene and the activity of the Drosophila EGF receptor (DER) signaling pathway are necessary to induce specifically three of the eight COPs in an embryonic abdominal hemisegment. The cell-lineage analysis of COPs using the yeast flpase (flp/FRT) method indicated that each of the eight COPs originated from an individual undifferentiated ectodermal cell. The eight COPs in each abdominal hemisegment seemed to be determined by a two-phase induction: first, five COPs are determined by the action of the proneural gene atonal and neurogenic genes. Subsequently, these five COPs start to express the rho gene, ...
Drosophila is an insect from the order Diptera, also called the fruit fly. The genus Drosophila includes about 400 species, found all over the planet. Life of the drosophila Drosophila flies...
Polyamine transport is elevated in many tumor types, suggesting that toxic polyamine-drug conjugates could be targeted to cancer cells via the polyamine transporter (PAT). We have previously reported the use of Chinese hamster ovary (CHO) cells and its PAT-deficient mutant cell line, CHO-MG, to screen anthracene-polyamine conjugates for their PAT-selective targeting ability. We report here a novel Drosophila-based model for screening anthracene-polyamine conjugates in a developing and intact epithelium (Drosophila imaginal discs), wherein cell-cell adhesion properties are maintained. Data from the Drosophila assay are consistent with previous results in CHO cells, indicating that the Drosophila epithelium has a PAT with vertebrate-like characteristics. This assay will be of use to medicinal chemists interested in screening drugs that use PAT for cellular entry, and it offers the possibility of genetic dissection of the polyamine transport process, including identification of a Drosophila PAT.
The cytokine-activated Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway plays an important role in the control of a wide variety of biological processes. When misregulated, JAK/STAT signaling is associated with various human diseases, such as immune disorders and tumorigenesis. To gain insights into the mechanisms by which JAK/STAT signaling participates in these diverse biological responses, we carried out a genome-wide RNA interference (RNAi) screen in cultured Drosophila cells. We identified 121 genes whose double-stranded RNA (dsRNA)-mediated knockdowns affected STAT92E activity. Of the 29 positive regulators, 13 are required for the tyrosine phosphorylation of STAT92E. Furthermore, we found that the Drosophila homologs of RanBP3 and RanBP10 are negative regulators of JAK/STAT signaling through their control of nucleocytoplasmic transport of STAT92E. In addition, we identified a key negative regulator of Drosophila JAK/STAT signaling, protein tyrosine ...
The relatively simple communication, breeding and egg-making systems that govern reproduction in female Drosophila retain homology to eusocial species in which these same systems are modified to the social condition. Despite having no parental care, division of labour or subfertile caste, Drosophila may nonetheless offer a living test of certain sociobiological hypotheses framed around gene function. In this review, we make this case, and do so around the recent discovery that the non-social fly, Drosophila melanogaster, can respond to the ovary-suppressing queen pheromone of the honey bee Apis meliffera. Here, we first explain the sociobiological imperative to reconcile kin theory with molecular biology, and qualify a potential role for Drosophila. Then, we offer three applications for the fly-pheromone assay. First, the availability and accessibility of massive mutant libraries makes immediately feasible any number of open or targeted gene screens against the ovary-inhibiting response. The sheer
Fissioncytorace-1, a member of the nasuta-albomicans complex of Drosophila is an evolutionary product of centric fission, which had occurred in the chromosome X3 of Cytorace 1, a hydridization product of Drosophila nasuta nasuta male (2n=8) and Drosophila nasuta albomicans female (2n=6). Cytorace 1 (males 2n=7; females 2n=6) has inherited this chromosome from its D. n. albomicans parent. The chromosome X3 of D. n. albomicans is a derivative of a centric fusion between the acrocentric chromosome 3 and the chromosome X of D. n. nasuta. The Fissioncytorace-1 has crossed 200 generations from the time of its evolution in the laboratory environment. When this centromeric fission race was subjected to some of the morphophenotypic and fitness assessment to find its overall population fitness showed, increased body size, sternopleural bristle, ovarioles, lifetime fecundity and fertility with reduced interspecific competitive ability and hatching success when compared with its parent (Cytorace 1). These ...
Applications are invited for a postdoc position and a full-time technician = position in Drosophila epigenetics research laboratory of Dr. Tulin at the = Fox Chase Cancer Center, Philadelphia, PA. Both positions planned for at = least three years, with possible renewal. The successful applicants will = use Drosophila model system to study epigenetics of development and = cancer. The primary research focus of Dr. Tulin=92s lab is on = fundamentals of chromatin reprogramming and RNA fate regulation during = normal development and carcinogenics, as well as on translating = fundamental research for clinical applications in oncology. Projects in = Dr. Tulin=92s lab cover the molecular mechanisms of the chromatin = remodeling and regulation of gene expression and employ Drosophila model = and in vitro assays as well as human cells, mouse models. Applicants for the postdoctoral position should have a Ph.D. in molecular = biology, molecular genetics, biochemistry, or a related field and 0-3 = years of ...
TY - JOUR. T1 - Effect of adult experience on oviposition choice and short-distance attraction in Drosophila buzzatii. AU - Hedrick, Philip W.. AU - Barker, J. S F. AU - Armstrong, T.. PY - 1990/9. Y1 - 1990/9. N2 - In a series of experiments, no consistent effect of adult experience, i.e., exposure to the naturally occurring yeasts, Candida sonorensis and Clavispora opuntiae, on oviposition choice or short-distance attraction in inbred lines of Drosophila buzzatii was found. The lack of consistent effect on oviposition choice was also found in one experiment in which the flies were starved and in another experiment in which choice was determined on 2 consecutive days.. AB - In a series of experiments, no consistent effect of adult experience, i.e., exposure to the naturally occurring yeasts, Candida sonorensis and Clavispora opuntiae, on oviposition choice or short-distance attraction in inbred lines of Drosophila buzzatii was found. The lack of consistent effect on oviposition choice was also ...
Drosophila suzukii Matsumura (Figure 1A) is one of the most serious pests of thin-skinned fruits including blueberry, raspberry, cherry, grape and strawberry [1â 5]. Mixtures of yeast, sugar, and water; fruit purees, distillates from apple cider vinegar or wine; ethanol, acetic acid, and phenylethanol in 1: 22: 5 ratios arâ ¦ It differs from other species of drosophilas by having a sawed oviscapto (organ used for laying eggs) that allows it to attack healthy fruits. Introduction. The spotted wing Drosophila (Drosophila suzukii) is an invasive and serious economic pest to small and stone fruits and its control is difficult. Another advantage of using a trap in this manner is that you can wait to treat your crops until you are sure you have the SWD on your property. The Spotted Wing Drosophila (SWD), Drosophila suzukii, is a small vinegar fly that damages many fruit crops. Although the spotted-wing drosophila Drosophila suzukii originates in Southeast Asia, this pest is now widespread in North ...
Assembly/Alignment/Annotation of 12 related Drosophila species: »Assembly/Alignment/Annotation, LBNL, USA BDGP Single Nucleotide Polymorphism (SNP) Project: »Berkeley Drosophila Genome Project, University of California, Berkeley, USA Berkeley Drosophila Genome Project (BDGP): »BDGP, University of California, Berkeley, USA BDTNP, ChIP/chip in vivo DNA binding data: »Berkeley Drosophila Transcription Network Project, University of California, Berkeley, USA CluSTr protein sequence similarity analysis of Drosophila: »CluSTr proteome analysis, EBI, UK D. pseudoobscura genome project: »Human Genome Sequencing Center, Baylor College of Medicine, USA D. simulans genome project: »Genome Sequencing Center, Washington University, USA D. yakuba genome project: »Genome Sequencing Center, Washington University, USA D.melanogaster UCSC Genome Browser Gateway: »University of California, Santa Cruz, USA DDBJ, the DNA Data Bank of Japan: »DDBJ, National Institute of Genetics, Mishima, Japan DNase I ...
The SpottedWingFlyBase is a dedicated online resource for Drosophila suzukii genomics. The recently introduced and rapidly spreading Spotted Wing Drosophila (Drosophila suzukii) has unique anatomy among Drosophila species that enables it to become a serious economic pest. Female D. suzukii has a serrated ovipositor and exhibits a preference for ovipositing in sound ripe and ripening fruit as opposed to the overripe fruit that other Drosophila species are known to infest. Since its initial detection in the continental United States in 2008 in the berry-growing central coastal region of California, significant crop losses have been reported not only in California, but also throughout the United States, Canada, and Europe among growers of berry crops and soft-skinned stone fruits.. The goal of our database and web server is to provide comparative genomics resources to enable and streamline basic research on Drosophila suzukii biology as well as applied research to develop effective monitoring and ...
TY - JOUR. T1 - Evolution of the LINE-like I element in the Drosophila melanogaster species subgroup. AU - Sezutsu, Hideki. AU - Nitasaka, Eiji. AU - Yamazaki, Tsuneyuki. PY - 1995/3/1. Y1 - 1995/3/1. N2 - LINE-like retrotransposons, the so-called I elements, control the system of I-R (inducer-reactive) hybrid dysgenesis in Drosophila melanogaster. I elements are present in many Drosophila species. It has been suggested that active, complete I elements, located at different sites on the chromosomes, invaded natural populations of D. melanogaster recently (1920-1970). But old strains lacking active I elements have only defective I elements located in the chromocenter. We have cloned I elements from D. melanogaster and the melanogaster subgroup. In D. melanogaster, the nucleotide sequences of chromocentral I elements differed from those on chromosome arms by as much as 7%. All the I elements of D. mauritiana and D. sechellia are more closely related to the chromosomal I elements of D. melanogaster ...
To study genes that may be crucial for the male germ cell development of Drosophila we screened a cDNA expression library with a polyclonal antiserum against testis proteins of Drosophila hydei. We identified a cDNA fragment that exhibited a complete sequence similarity with the cDNA of the laminin B2 chain, an important component of the extracellular matrix. Transcripts of laminin B2 were detected in the RNA of male germ cells with the polymerase chain reaction and by in situ hybridization. We studied the reaction of different polyclonal antibodies including those against a Drosophila laminin B2-lac fusion protein, the entire Drosophila laminin complex, or against the mouse laminin complex and against laminin A and B1 chains with specific structures in developing male germ cells of Drosophila. Antigenic sites against laminin B2 were found in the lampbrush loops in primary spermatocyte nuclei, in nuclei of spermatids, and in heads of spermatozoa. The axonemes of elongating spermatids react with ...
Lab Reagents Drosophila Antibody Laboratories manufactures the antibody for drosophila hemocytes reagents distributed by Genprice. The Antibody For Drosophila Hemocytes reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact drosophila Antibody. Other Antibody products are available in stock. Specificity: Antibody Category: For Group: Drosophila Hemocytes. Drosophila Hemocytes information ...
Domain architectures containing the following SCOP superfamilies 54236,47031,50729,_gap_ in Drosophila mojavensis 1.3. Domain architectures illustrate each occurrence of 54236,47031,50729,_gap_.
Dedicated to the memory of George Lefevre in recognition of his exhaustive cytogenetic analysis of the X chromosome, |b|The Genome of Drosophila melanogaster|/b| is the complete compendium of what is known about the genes and chromosomes of this widely used model organism. The volume is an up-to-date revision of Lindsley and Grells 1968 work, Genetic Variations of Drosophila melanogaster. The new edition contains complete descriptions of normal and mutant genes including phenotypic, cytological, molecular, and bibliographic information. In addition, it describes thousands of recorded chromosome rearrangements used in research on Drosophila. This handbook and its accompanying polytene chromosome maps, are sturdily bound into the book as foldouts and available as a separate set, are essential research tools for the Drosophila community.|br||br|Key Features |br|* Describes phenotype, cytology, and molecular biology of all recorded genes of Drosophila melanogaster, plus references to the literature|br
VALIATI, Victor Hugo and VALENTE, Vera Lucia S.. Chromosomal polymorphism in urban populations of Drosophila paulistorum. Braz. J. Genet. [online]. 1997, vol.20, n.4, pp.-. ISSN 0100-8455. http://dx.doi.org/10.1590/S0100-84551997000400004.. Drosophila paulistorum populations colonizing the urban area of Porto Alegre, southern Brazil, were studied with the objective of characterizing their chromosomal polymorphism in this new environment. Despite being geographically and ecologically marginal and the fact that the colonization of the urban area seems to be a recent event, the populations showed a large number of inversions on all chromosome arms. Differences regarding inversion frequencies and percentage of heterozygosis were found when we compared the samples with respect to geographical, microenvironmental and temporal aspects. Such differences, however, could be attributed to both selective and stochastic factors. ...
TY - JOUR. T1 - The initiation of pair-rule stripes in the Drosophila blastoderm. AU - Small, Stephen. AU - Levine, Michael. PY - 1991/8. Y1 - 1991/8. N2 - The interactions between the products of gap genes and pair-rule promoters results in the single most dramatic increase in the spatial complexity of gene expression during the segmentation process. We attempt to relate recent findings on the regulation of striped patterns of gene expression in the early Drosophila embryo to general strategies of gene expression and development employed by higher organisms.. AB - The interactions between the products of gap genes and pair-rule promoters results in the single most dramatic increase in the spatial complexity of gene expression during the segmentation process. We attempt to relate recent findings on the regulation of striped patterns of gene expression in the early Drosophila embryo to general strategies of gene expression and development employed by higher organisms.. UR - ...
Electrophysiological analysis of cultured neurons provides a potential approach toward understanding the physiological defects that may contribute to abnormal behavior exhibited by mutants of the fruit fly Drosophila. However, its application has been restricted by an inability to identify a particular functional or anatomical subpopulation of neurons from the CNS. To study neurons composing the CNS mushroom body proposed as a center for insect olfactory learning, we utilized a Drosophila enhancer detector line that expresses a lacZ reporter gene in these neurons and identified them in acutely dissociated larval CNS cultures by vital fluorescent staining. The patch-clamp analysis suggests that whole-cell voltage-activated K+ currents can be classified into two types in identified mushroom body neurons. Type 1 current comprises a TEA-sensitive slowly inactivating current and noninactivating component while type 2 current contains a 4-AP-sensitive transient A-current and a noninactivating ...
P-13. Transcription Profiling of Cell Death in Drosophila Melanogaster. S. Chittaranjan, E. Garland, D. Freeman, S. Jones, M. Marra, and S. Gorski, Genome Sequence Centre BC Cancer Agency, Vancouver, CANADA. Programmed Cell death - PCD - is a highly conserved and genetically controlled event that plays important roles in animal development, homeostasis and disease. We are employing a genomics approach, using EST and SAGE, to identify new PCD genes that are transciptionally regulated in the salivary glands - SGs - of Drosophila. We constructed a SG-specific cDNA library and SG-miniSAGE libraries from three consecutive developmental stages leading up to PCD.5461 high quality 3 ESTs from our SG cDNA library were used to perform BLAST analysis against Drosophila predicted genes and genomic sequence from the Drosophila Genome Project. The majority of the ESTs matched known or predicted genes in Drosophila including a number of ecdysone-induced genes and known PCD genes. We also identified a ...
The Huntingtons disease (HD) gene encodes a protein, huntingtin, with no known function and no detectable sequence similarity to other proteins in current databases. To gain insight into the normal biological role of huntingtin, we isolated and sequenced a cDNA encoding a protein that is a likely homolog of the HD gene product in Drosophila melanogaster. We also determined the complete sequence of 43,125 contiguous base pairs of genomic DNA that encompass the Drosophila HD gene, allowing the intron-exon structure and 5- and 3-flanking regions to be delineated. The predicted Drosophila huntingtin protein has 3583 amino acids, which is several hundred amino acids larger than any other previously characterized member of the HD family. Analysis of the genomic and cDNA sequences indicates that Drosophila HD has 29 exons, compared with the 67 exons present in vertebrate HD genes, and that Drosophila huntingtin lacks the polyglutamine and polyproline stretches present in its mammalian counterparts. ...
TY - JOUR. T1 - The M/SAR elements of the bithorax complex in Drosophila melanogaster. AU - Boykova, T. V.. AU - Orlando, V.. AU - Lupo, R.. AU - Bogachev, S. S.. PY - 2005/11. Y1 - 2005/11. N2 - The bithorax (BX) complex of Drosophila is a complex polygenic region with a multifactorial system of regulation. One of the levels of the regulatory system of the BX complex is its association with the nuclear skeleton structures through a specific interaction of the M/SAR DNA with the nuclear matrix proteins. In the present work, M/SAR elements were mapped on the molecular-genetic map of the region. All of the elements examined were found to colocalize with regulatory elements and form clusters that restrict/bracket the genetically active domains. All M/SAR DNA revealed was shown to bins specifically to the purified Drosophila melanogaster lamin.. AB - The bithorax (BX) complex of Drosophila is a complex polygenic region with a multifactorial system of regulation. One of the levels of the regulatory ...
The Vienna Drosophila Resource Center (VDRC) is a professionally organized bioresource center of international significance that aims to promote scientific discoveries in Drosophila melanogaster. Our primary aim is to maintain unique transgenic Drosophila stocks and DNA resources and to distribute them both locally and worldwide. We also acquire, create, and develop new resources, according to emerging new technologies and the needs of the local and international Drosophila research community.. ...
Semantic Scholar extracted view of Biochemical phylogeny of the eight species in the Drosophila melanogaster subgroup, including D. sechellia and D. orena. by M L Cariou
Abdelwahid, E., Yokokura, T., Krieser, R. J., Balasundaram, S., Fowle, W. H. and White, K. (2007). Mitochondrial disruption in Drosophila apoptosis. Dev. Cell 12(5): 793-806. PubMed citation: 17488629 Abrams, J. M., et al. (1993). Programmed cell death during Drosophila embryogenesis. Development 117: 29-43. PubMed Citation: 8223253 Asano, M, Nevins, J. R. and Wharton, R. P. (1996). Ectopic E2F expression induces S phase and apoptosis in Drosophila imaginal discs. Genes Dev. 10: 1422-32. PubMed Citation: 8647438 Avdonin, V., et al. (1998). Apoptotic proteins Reaper and Grim induce stable inactivation in voltage-gated K+ channels. Proc. Natl. Acad. Sci. 95(20): 11703-8. PubMed Citation: 9751729 Baer, M. M., Bilstein, A., Caussinus, E., Csiszar, A., Affolter, M. and Leptin, M. (2010). The role of apoptosis in shaping the tracheal system in the Drosophila embryo. Mech. Dev. 127(1-2): 28-35. PubMed Citation: 19995601 Bardet, P. L. et al. (2008). A fluorescent reporter of caspase activity for live ...
Males of this genus are known to have the longest sperm cells of any organism on Earth.[5] One species, Drosophila bifurca, has sperm 58 mm (2.3 in) long.[5][6][7] The cells are mostly tail, and are delivered to the females in tangled coils. Those Drosophila species with very long sperms make relatively few sperm cells.[8] D. melanogaster sperm cells are a more modest 1.8 mm long, although this is still about 300 times longer than a human sperm. Several species in the D. melanogaster species group are known to mate by traumatic insemination, in which the male pierces the females abdomen with his penis and injects his sperm through the wound into her abdominal cavity (haemocoel).[9][10]. Drosophila vary widely in their reproductive capacity. Those such as D. melanogaster that breed in large, scarce resources have ovaries that mature 10-20 eggs at a time, and can be laid together in one place. Others, which breed in common but less nutritious places (such as leaves), may only lay one egg each ...
TY - JOUR. T1 - Cloning, heterologous expression and developmental regulation of a Drosophila receptor for tachykinin-like peptides. AU - Li, X. J.. AU - Wolfgang, W.. AU - Wu, Y. N.. AU - North, R. A.. AU - Forte, M.. PY - 1991. Y1 - 1991. N2 - We identified clones encoding a Drosophila receptor for tachykinin-like peptides by low stringency screening of an embryonic cDNA library with probes from the bovine substance K receptor. The cDNAs encode a seven transmembrane domain protein (DTKR) of 519 amino acids with 40-48% amino acid identity to mammalian tachykinin receptors within transmembrane regions. Xenopus oocytes injected with DTKR cRNAs showed selective responses to vertebrate substance P, its agonists and not to other vertebrate tachykinin peptides. These responses were eliminated by treatment of oocytes with pertussis toxin. In the adult fly, Northern and PCR analysis demonstrated preferential expression of DTKR in the head; in situ hybridization indicated that DTKR is accumulated in the ...
TY - JOUR. T1 - Localization of transcripts from the wingless gene in whole Drosophila embryos. AU - Baker, N. E.. PY - 1988. Y1 - 1988. N2 - In situ hybridization has been used to detect transcription in whole Drosophila embryos. Improved results have been obtained by incubating the hybridized embryos in liquid emulsion prior to autoradiographic exposure. This technique has been used to map the distribution of transcripts from the segment-polarity gene wingless (wg), which is transcribed in a stripe in each segment of the trunk region. By the extended germband stage additional areas of transcription in the head and caudal regions define a total of 21 domains, comprising the foregut, six regions in the head, three thoracic and ten abdominal segments, and the hindgut. At the end of the extended germband stage, the pattern of wg transcription is no longer uniform in the dorsoventral axis: wg transcripts are absent from the lateral epidermis. This pattern of wg transcription is discussed with ...
The Notch receptor of Drosophila and its homologues in other organisms mediate cell-cell interactions required for the correct partitioning of cell fates within equivalence groups. Genes related to Notch and other components of the Notch signaling pathway represent a well conserved system for signal transduction, having been isolated from organisms as diverse as flies, worms, sea urchins, frogs, fish, chickens, mice, rats, and humans (reviewed by Lardelli et al., 1995). The expression and requirements for Notch signaling are pleiotropic through development, in contrast to other tissue or cell type specific receptors. How the Notch signaling cascade mediates pattern formation in so many tissues and cell types is not well understood. The research contained herein increases the understanding of Notch signaling by studying its role during Drosophila oogenesis. Additionally, this research lends insight into several important processes that take place during Drosophila oogenesis, including ...
2011 Nobel Laureate Jules Hoffmann, Ph.D. described his scientific journey including the discovery of Toll receptors and innate immunity in the keynote lecture on Apr. 3, 2013, the opening night of the Genetics Society of Americas 54th Annual Drosophila Research Conference in Washington, D.C., Apr. 3-7, 2013.
Humanised fruit fly models are transgenic Drosophila melanogaster strains expressing human genes. Specifically, we study the characteristics of fly models expressing human genes involved in neurological disorders such as Alzheimers and Parkinsons Diseases (AD and PD). Interestingly, these Drosophila neurodegenerative disease models show a high degree of conservation in the fundamental biological pathways and in the molecular, genetic and pathophysiological aspects of neurodegenerative human diseases. These characteristics explain why Drosophila models have paved the way for the development of initial fast screening for potential drug candidates in vivo, and represent also a promising tool for biomedical research in neuroscience ...
Comparative Analysises and Genome Databases= =Sequence Traces of Drosophila Genomes= =Genetic and Protein Interaction Databases= =Sequence Analysis= =Atlases, Images, and Videos= =Other= =Related Projects= :[http://www.gmod.org/ GMOD] :[http://www.geneontology.org/ Gene Ontology (GO)] :[http://www.genomeknowledge.org/ Reactome] :[http://www.fruitfly.org/ BDGP (Berkeley Drosophila Genome Project)] :[http://dgrc.cgb.indiana.edu/ DGRC (Drosophila Genomics Resource Center)] :[http://www.ou.edu/journals/dis/byissue.html DIS by issue] :[http://www.flyexpress.net/ FlyExpress] :[http://www.sdbonline.org/fly/aimain/1aahome.htm Interactive Fly] :[http://www.modencode.org/ modENCODE] :[http://www.textpresso.org/fly/ Textpresso for Fly] =Stock Collections= ===D. melanogaster Collections=== :[http://flystocks.bio.indiana.edu/ Bloomington] :[http://www.drosdel.org.uk/ DrosDel] :[http://drosophila.med.harvard.edu/ Exelixis (Harvard)] :[http://flypush.imgen.bcm.tmc.edu/pscreen/ GDP (Baylor)] ...
Drosophila melanogaster, the common fruit fly, has been used as a model organism in both medical and scientific research for over a century. Work by Thomas Hunt Morgan (1866-1945) and his students at Columbia University at the beginning of the twentieth century led to great discoveries such as sex-linked inheritance and that ionising radiation causes mutations in genes. However, the use of Drosophila was not limited to genetic research. Experimentation with this model organism has also led to discoveries in neuroscience and neurodevelopment, including the basis of circadian rhythms. Its complex nervous system, conserved neurological function, and human disease-related loci allow Drosophila to be an ideal model organism for the study of neurodegenerative disease, for which it is used today, aiding research into diseases such as Alzheimers and Parkinsons, which are becoming more prevalent in todays ageing population.. Keywords Drosophila melanogaster, Thomas Hunt Morgan, Alfred Henry ...
Fingerprint Dive into the research topics of Daughterless is required for Drosophila photoreceptor cell determination, eye morphogenesis, and cell cycle progression. Together they form a unique fingerprint. ...
We report the complete sequence of a calcium channel alpha 1 subunit cDNA cloned from a Drosophila head cDNA library. This cDNA encodes a deduced protein containing 2516 amino acids with a predicted molecular weight of 276,493. The deduced protein shares many features with vertebrate homologs, including four repeat structures, each containing six transmembrane domains, a conserved ion selectivity filter region between transmembrane domains 5 and 6, and an EF hand in the carboxy tail. The Drosophila subunit has unusually long initial amino and terminal carboxy tails. The region corresponding to the last transmembrane domain (IVS6) and the adjacent cytoplasmic domain has been postulated to form a phenylalkylamine-binding site in vertebrate calcium channels. This region is conserved in the Drosophila sequence, while domains thought to be involved in dihydropyridine binding show numerous changes. The Drosophila subunit exhibits 78.3% sequence similarity to the rat brain type D calcium channel alpha ...
Rab proteins are small GTPases that play important roles in transport of vesicle cargo and recruitment, association of motor and other proteins with vesicles, and docking and fusion of vesicles at defined locations. In vertebrates, ,75 Rab genes have been identified, some of which have been intensively studied for their roles in endosome and synaptic vesicle trafficking. Recent studies of the functions of certain Rab proteins have revealed specific roles in mediating developmental signal transduction. We have begun a systematic genetic study of the 33 Rab genes in Drosophila. Most of the fly proteins are clearly related to specific vertebrate proteins. We report here the creation of a set of transgenic fly lines that allow spatially and temporally regulated expression of Drosophila Rab proteins. We generated fluorescent protein-tagged wild-type, dominant-negative, and constitutively active forms of 31 Drosophila Rab proteins. We describe Drosophila Rab expression patterns during embryogenesis, ...
Applications are invited for one post of Junior Research Fellow for the ongoing DBT project entitled Unraveling the adaptive genome evolution by whole genome sequencing of Drosophila nasuta nasuta and laboratory evolved four hybrid strains, Cytoraces for the remaining period of 2 years.. Position Title: Junior Research Fellow. Project Title: Unraveling the adaptive genome evolution by whole genome sequencing of Drosophila nasuta nasuta and laboratory evolved four hybrid strains, Cytoraces. Qualifications: JRF position - I or II class (Above 55%) M.Sc. in Genetics/ Zoology/ Genomics/ Bioinformatics. NET/GATE exam qualified candidates or Non- NET candidates with experience in handling Drosophila and also research experience in the genome analysis will be preferred.. Duration: 2 years. Fellowship: The value of the fellowship would be as per DBT regulations.. How To Apply:. The eligible candidates may send application on plain paper with a biodata along with copies of marks cards, certificates, ...
TY - JOUR. T1 - Signaling mechanisms in induction of the R7 photoreceptor in the developing Drosophila retina. AU - Yamamoto, Daisuke. PY - 1994/4. Y1 - 1994/4. N2 - The Drosophila compound eye is an excellent experimental system for analysing fate induction of identifiable single cells. Each ommatidium, a unit eye, contains eight photoreceptors (R1‐R8), and the differentiation of these photoreceptors occurs in the larval eye imaginal disc in discrete steps: first R8 is determined, then R2/R5, R3/R4, R1/R6 and finally R7. Induction of R7, in particular, has been extensively studied at the molecular level. The R8 photoreceptor presents on its surface a ligand, Bride of Sevenless, that binds and activates Sevenless receptor tyrosine kinase in the R7 precursor. Autophosphorylated Sevenless initiates a Ras1‐mediated cascade, which eventually activates transcription factors in the nucleus via Raf1 and MAP kinases, resulting in R7 development. However, recent studies indicate that Sevenless (Sev) ...
Drosophila nikananu adolah saikua langau dari genus Drosophila. Spesies ko juo marupokan bagian dari famili Drosophila, ordo Diptera, kelas Insecta, filum Arthropoda, dan kingdom Animalia. Istilah Drosophila, baarati panyuko ambun, adolah adaptasi saintifik Latin modern dari kato Yunani δρόσος, drósos, ambun, dan δρόσος, drósos, panyuko, nan ditambahkan jo akiaran Latin feminin -a. ...
Drosophila paratarsata adolah saikua lalek dari genus Drosophila. Spesies ko juo marupokan bagian dari famili Drosophila, ordo Diptera, kelas Insecta, filum Arthropoda, dan kingdom Animalia. Istilah Drosophila, baarati panyuko ambun, adolah adaptasi saintifik Latin modern dari kato Yunani δρόσος, drósos, ambun, dan δρόσος, drósos, panyuko, nan ditambahkan jo akiaran Latin feminin -a. ...
TY - JOUR. T1 - Drosophila in cancer researchan expanding role. AU - Potter, Christopher J.. AU - Turenchalk, Gregory S.. AU - Xu, Tian. N1 - Funding Information: We thank Karen Wehner and members of the Xu Lab for helpful comments. This work was supported by NIH grant RO1CA69408. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 2000/1/1. Y1 - 2000/1/1. N2 - In recent years, Drosophila researchers have developed powerful genetic techniques that allow for the rapid identification and characterization of genes involved in tumor formation and development. The high level of gene and pathway conservation, the similarity of cellular processes and the emerging evidence of functional conservation of tumor suppressors between Drosophila and mammals, argue that studies of tumorigenesis in flies can directly contribute to the understanding of human cancer. In this review, we explore the historical and current roles of Drosophila in cancer research, as well as speculate on the future of ...