Background Assessment of the biodiversity of communities of small organisms is most readily done using PCR-based analysis of environmental samples consisting of mixtures of individuals. Known as metagenetics, this approach has transformed understanding of microbial communities and is beginning to be applied to metazoans as well. Unlike microbial studies, where analysis of the 16S ribosomal DNA sequence is standard, the best gene for metazoan metagenetics is less clear. In this study we designed a set of PCR primers for the mitochondrial 12S ribosomal DNA sequence based on 64 complete mitochondrial genomes and then tested their efficacy. Methodology/Principal Findings A total of the 64 complete mitochondrial genome sequences representing all metazoan classes available in GenBank were downloaded using the NCBI Taxonomy Browser. Alignment of sequences was performed for the excised mitochondrial 12S ribosomal DNA sequences, and conserved regions were identified for all 64 mitochondrial genomes. These
Chronic wounds affect millions of people and cost billions of dollars in the United States each year. These wounds harbor polymicrobial biofilm communities, which can be difficult to elucidate using culturing methods. Clinical molecular microbiological methods are increasingly being employed to investigate the microbiota of chronic infections, including wounds, as part of standard patient care. However, molecular testing is more sensitive than culturing, which results in markedly different results being reported to clinicians. This study compares the results of aerobic culturing and molecular testing (culture-free 16S ribosomal DNA sequencing), and it examines the relative abundance score that is generated by the molecular test and the usefulness of the relative abundance score in predicting the likelihood that the same organism would be detected by culture. Parallel samples from 51 chronic wounds were studied using aerobic culturing and 16S DNA sequencing for the identification of bacteria. One hundred
Strain D-14T, a brown-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from oil-contaminated soil. It was able to grow at 20-40 °C, at pH 6.0-10.0 and at 0-1 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain D-14T belonged to the genus Lysobacter and was closely related to Lysobacter caeni BUT-8T (99.0 % sequence similarity), Lysobacter ruishenii CTN-1T (98.5 %), Lysobacter daejeonensis GH1-9T (98.2 %) and Lysobacter panacisoli CJ29T (97.2 %). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl-N-methyl-ethanolamine. The predominant fatty acids of strain D-14T were iso-C15 : 0, iso-C16 : 0, summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl), summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), iso-C14 : 0, C11 : 0iso 3-OH, C15 : 1iso F and C16 : 0. The genomic DNA G+C content of this novel strain
The nuclear ribosomal repeats for the 18S, 5.8S, and 26S RNAs of two closely related Picea (spruce) species were characterized by restriction mapping and Southern blot hybridization. Restriction polymorphisms were identified in the IGS and ITS sequences; however, no polymorphism was species specific. As many as five different rDNA repeat units were observed in individual genomes. The repeat size for these gymnosperms ranged from a minimum of 32 kbp to greater than 40 kbp, two- to threefold larger than the typical angiosperm rDNA unit. Slot-blot hybridizations were used to determine the nuclear rDNA copy concentration. Among P. rubens individuals threefold variation was observed in the rDNA copy concentration, and among P. mariana individuals such variation was as much as sixfold. At a size greater than 32 kbp and at a concentration averaging 1.2-1.3 x 10(4) copies/pg, the rDNA constitutes approximately 4% of the total genome. Regression analysis revealed a significant relationship between copy
Vol 63: Ribosomal DNA Sequence Heterogeneity Reflects Intraspecies Phylogenies and Predicts Genome Structure in Two Contrasting Yeast Species.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Vol 13: Molecular identification of clinical difficult-to-identify microbes from sequencing 16S ribosomal DNA and internal transcribed spacer 2.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Ben Hanelt, D. Van Schyndel, C. M. Adema, L. A. Lewis, E. S. Loker. The Phylogenetic Position of Rhopaluva ophiocomae (Orthonectida) Based on 18s Ribosomal DNA Sequence Analysis. -Molecular Biology and Evolution, 1996,. 13 (9), lk 1187-1191. Veebiversioon. ...
Learn about Exubera (Insulin Human [rDNA origin]) may treat, uses, dosage, side effects, drug interactions, warnings, patient labeling, reviews, and related medications.
Aime, M. C., P. B. Matheny, D. A. Henk, E. M. Frieders, R. H. Nilsson, M. Piepenbring, D. J. McLaughlin, L. J. Szabo, D. Begerow, J. P. Sampaio, R. Bauer, M. Wei , F. Oberwinkler, and D. S. Hibbett. 2006. An overview of the higher-level classification of Pucciniomycotina based on combined analyses of nuclear large and small subunit rDNA sequences. Mycologia 98: 896 905.. Bauer, R., D. Begerow, J. Sampaio, M. Weiβ, F. Oberwinkler. 2006. The simple-septate basidiomycetes: a synopsis. Mycological Progress 5: 41 66.. Hibbett, D. S., M. Binder, J. F. Bischoff, M. Blackwell, P. F. Cannon, O. E. Eriksson, S. Huhndorf, T. James, P. M. Kirk, R. L cking, T. Lumbsch, F. Lutzoni, P. B. Matheny, D. J. Mclaughlin, M. J. Powell, S. Redhead, C. L. Schoch, J. W. Spatafora, J. A. Stalpers, R. Vilgalys, M. C. Aime, A. Aptroot, R. Bauer, D. Begerow, G. L. Benny, L. A. Castlebury, P. W. Crous, Y.-C. Dai, W. Gams, D. M. Geiser, G. W. Griffith, C. Gueidan, D. L. Hawksworth, G. Hestmark, K. Hosaka, R. A. Humber, K. ...
Strange looking elevated amplification plots from ChIP-qPCR samples - posted in PCR, RT-PCR and Real-Time PCR: Hi everyone, I am hoping that you all can help as I am not an expert in qPCR. I performed a ChIP experiment to assess binding to human ribosomal gene repeats. I used primers that have been published in multiple papers and a positive ChIP control: UBF. I have attached the strange amplification plots that I get when doing a standard curve using a dilution series of 1:10 of...
背景:临床真菌引起的血流感染日益增加,其中念珠菌属引起的感染占真菌感染的90.0%以上,主要包括白色念珠菌(66.0%)、光滑念珠菌(11.2%)、热带念珠菌(7.6%)、近平滑念珠菌(5.6%)和克柔念珠菌(2.4%) 5种念珠菌,占临床念珠菌属感染的90.0%以上。目前,检测和鉴定念珠菌属/种血流感染主要依赖血培养和血清学试验,但固有的方法学缺陷难以满足临床快速、准确鉴定血流感染的需要。. 目的:分别建立念珠菌属和5种念珠菌(白色念珠菌、光滑念珠菌、近平滑念珠菌、热带念珠菌和克柔念珠菌)的real-time PCR快速检测平台,并对所建方法及其临床应用价值进行初步评价。. 方法:. 1.引物和探针设计:分别以上述5种念珠菌标准菌株的5.8S rRNA 基因(5.8S rDNA)序列和内转录间隔序列(ITS)作为参考序列,通过属、种间序列比对,在5.8S ...
Addgene NGS Result TTAATGATTAACCCGCCATGCTACTTATCTACGTAGCCATGCTCTAGGAAGATCCAACATATCCTGGTGT GGAGTAGGGGACGCTGCTCTGACAGAGGCTCGGGGGCCTGAGCTGGCTCTGTGAGCTGGGGAGGAGGCAG ACAGCCAGGCCTTTGTCTGCAAGCAGACCTGGCAGCATTGGGCTGGCCGCCCCCCAGGGCCTCCTCTTCA TGCCCAGTGAATGACTCACCTTGGCACAGACACAATGTTCGGGGTGGGCACAGTGCCTGCTTCCCGCCGC ACCCCAGCCCCCCTCAAATGCCTTCCGAGAAGCCCATTGAGCAGGGGGCTTGCATTGCACCCCAGCCTGA CAGCCTGGCATCTTGGGATAAAAGCAGCACAGCCCCCTAGGGGCTGCCCTTGCTGTGTGGCGCCACCGGC GGTGGAGAACAAGGCTCTATTCAGCCTGTGCCCAGGAAAGGGGATCAGGGGATGCCCAGGCATGGACAGT GGGTGGCAGGGGGGGAGAGGAGGGCTGTCTGCTTCCCAGAAGTCCAAGGACACAAATGGGTGAGGGGAGA GCTCTCCCCATAGCTGGGCTGCGGCCCAACCCCACCCCCTCAGGCTATGCCAGGGGGTGTTGCCAGGGGC ACCCGGGCATCGCCAGTCTAGCCCACTCCTTCATAAAGCCCTCGCATCCCAGGAGCGAGCAGAGCCAGAG CAGGTTGGAGAGGAGACGCATCACCTCCGCTGCTCGCGGGGATCCCGCCACCATGGAGACAGACACACTC CTGCTATGGGTACTGCTGCTCTGGGTTCCAGGTTCCACTGGTGACAGATCTGCCGCAGGCAGCACGCTGG ACAAAATCGCCAAAAACGGTGTGATTGTCGTCGGTCACCGTGAATCTTCAGTGCCTTTCTCTTATTACGA ...
Abstract: 对大连某城市污水处理厂活性污泥进行长期驯化,筛选得到好氧条件下以对氨基苯磺酸(4-aminobenzenesulphonate,4-ABS)为唯一碳源和能源生长的高效降解菌株W1.根据菌株W1的形态特征、生理生化特征及16S rDNA序列分析,初步鉴定为Pannonibacter菌属.通过考察生长条件对降解效果的影响,确定了该菌株降解4-ABS的优化条件为:接种量10%、 30℃、 pH 7、摇床转速150 r/min,并且在有外加碳源的情况下仍保持较高的4-ABS降解活性.在4-ABS的降解过程中,4-ABS自身含有的氨基和磺酸基会以NH+4和SO2-4的形式释放到水体中,但浓度仅为理论释放量的77.6%和91.5%,推测原因是部分释放的NH+4和SO2-4被菌株W1作为氮源和硫源利用;菌株W1可以耐受2 500 mg/L的4-ABS,且在32 ...
هدف: نگرانی از وجود آفلاتوکسین در مواد غذایی و خطراتی که این سم برای سلامت انسان و حیوانات دارد، باعث پیدایش راه‌های مختلف حذف یا کاهش این سم شده است؛ از جمله این روش‌ها، کنترل زیستی قارچ توسط ریززنده‌های دیگر است. در این تحقیق از باکتری باسیلوس آمیلولیکوفاسینس جدا شده از خاک باغ پسته از باغات شهرستان دامغان به‌عنوان عامل کنترل زیستی برای مهار رشد و تولید آفلاتوکسین قارچ آسپرژیلوس پارازیتیکوس استاندارد NRRL2999 استفاده شد. مواد و روش‌ها: پس از 72 ساعت کشت باکتری در دمای 30 درجه سانتی‌گراد، مایع‌رویی آن به‌عنوان منبع ترکیبات ضد قارچی جداسازی شد. غلظت‌های
A polyphasic study was carried out to clarify the taxonomic position of two Gram-positive bacteria isolated from soil samples of the Grotta dei Cervi (Italy), a relatively unexplored hypogean environment. The strains, 20-5T and 23-23T, showed phenotypic and phylogenetic characteristics that were consistent with their classification in the genus Agromyces. 16S rRNA gene sequence comparisons revealed that the two strains formed distinct phyletic lines within the genus Agromyces. Based on 16S rRNA gene sequence similarity, chemotaxonomic data and the results of DNA-DNA relatedness studies, it is proposed that the two isolates represent two novel species of the genus Agromyces. Pronounced differences in a broad range of phenotypic characteristics and DNA G+C content distinguished the two strains from each other and from previously described species of the genus Agromyces. Two novel species are proposed: Agromyces salentinus sp. nov. (type strain, 20-5T=HKI 0320T=DSM 16198T=NCIMB 13990T) and Agromyces
A novel Ferrimonas species is described on the basis of phenotypic, chemotaxonomic and phylogenetic studies. Four halophilic organisms were isolated from marine sand and marine macroalgae samples by using high-pH marine agar 2216. An analysis of the nearly complete 16S rRNA gene sequences of these new isolates indicated that they were phylogenetically close (16S rRNA gene sequence similarity |99·5 %, gyrB gene sequence similarity |97·8 %), and were most closely related to Ferrimonas balearica (16S rRNA gene sequence similarity 97·1-97·3 %, gyrB gene sequence similarity 84·4-85·0 %). Chemotaxonomic data (major menaquinone MK7; major fatty acids C16 : 0 and C18 : 1 ω9c) supported the affiliation of the new isolates to the genus Ferrimonas. The results of physiological and biochemical tests allowed phenotypic differentiation of the isolates from F. balearica. It is therefore proposed that the new isolates represent a novel species with the name Ferrimonas marina sp. nov. and type strain A4D-4T (
A Gram-stain-positive, aerobic, non-motile, rod-shaped bacterium, strain 0704C9-2T, was isolated from hydrothermal sediment of the Indian Ocean. The organism grew with 0-5 % (w/v) NaCl and at 10-37 °C, with optimal growth occurring with 1 % NaCl and at 28-30 °C. Comparative 16S rRNA gene sequence analysis revealed that strain 0704C9-2T belonged to the genus Microbacterium . It exhibited highest 16S rRNA gene sequence similarity with Microbacterium testaceum DSM 20166T (98.4 %). Levels of similarity with the type strains of all other recognized species of the genus Microbacterium were less than 98.0 %. DNA-DNA hybridization experiments with strain 0704C9-2T and its closest relative, M. testaceum DSM 20166T, revealed a low reassociation value of 42.9 %. The DNA G+C content of strain 0704C9-2T was 73.3 mol%. The cell-wall peptidoglycan contained ornithine and the acyl type was glycolyl. The major whole-cell sugars were mannose, galactose, rhamnose and glucose. The major cellular fatty acids were
A bacterial strain, B5-2(T), was isolated from an ice core drilled from Muztagh Glacier, China. Strain B5-2(T) was a Gram-stainnegative, short rod-shaped, motile by polar flagella, aerobic bacterium. The major fatty acids of strain B5-2(T) were summed feature 8 (C-18 : 1 omega 7c and/ or C-18 : 1 omega 6c) and iso-C-13 : 0. The G+C content of the DNA from strain B5-2(T) was 69.3 mol%. The predominant isoprenoid quinone of strain B5-2(T) was Q-10. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine, an unidentified phospholipid and sulfoquinovosyldiacylglycerol. Comparative 16S rRNA gene sequence analysis revealed that the novel strain B5-2(T) shared highest similarity (96.7 %) with Aureimonas altamirensis S21B(T). On the basis of the results of this polyphasic study, strain B5-2(T) represents a novel species of the genus Aureimonas, for which the name Aureimonas glaciei sp. nov. is ...
The identification of mycobacteria can be a complicated, expensive, and difficult process; many laboratories are now referring uncommon organisms to laboratories that have the capability of using additional technology. Nucleic acid probes have offered laboratories the ability to rapidly and accurately identify four of the most common mycobacterial species, and they have rarely misidentified an organism (3).. A more important issue is the inaccuracy of phenotypic methods in providing a reliable and timely identification of the other mycobacteria (14). Nucleic acid sequencing of 16S rDNA has been investigated as a definitive method for the identification of many microorganisms, including mycobacteria (3, 5, 8, 11, 12, 16-18, 23), and its use is becoming more extensive. Most of the studies used a small number of organisms for evaluation or included only common species and/or the type species from culture collections.. We sought to determine whether 16S rDNA sequencing with a commercially available ...
Community Structure, Diversity, and Vertical Distribution of Archaea Revealed by 16S rRNA Gene Analysis in the Deep Sea Sediment of the Ulleung Basin, East Sea - archaeal diversity;16S rRNA gene;marine group;Ulleung Basin;East Sea;
Widdel 1981) Kuever 2006 may be the type and only species of the genus and the order GEBAproject. class represents a separate lineage within the which is only distantly related to most other members of this class. The closest relatives based on 16S rRNA gene sequence similarity values are the type strains of (87.6% sequence identity) and (87.2%) both belonging to the family within the order [9]. The most similar cloned 16S rRNA gene EUB-42 [10] shared only 95.5% sequence similarity with and was retrieved from anaerobic sludge. Strain 2st14T WYE-354 represents the only stress of this varieties obtainable from a tradition collection so far. Available data from cultivation 3rd party studies (environmental testing and genomic studies) didnt surpass 86% series similarity indicating that people of this varieties are limited to specific habitats which are undersampled generally in most conditions or are in low great quantity (status Oct 2010). The solitary genomic 16S rRNA series of stress 2st14T was ...
Bacillus tusciae Bonjour & Aragno 1994 is a hydrogen-oxidizing, thermoacidophilic spore former that lives as a facultative chemolithoautotroph in solfataras. Although 16S rRNA gene sequencing was well established at the time of the initial description of the organism, 16S sequence data were not available and the strain was placed into the genus Bacillus based on limited chemotaxonomic information. Despite the now obvious misplacement of strain T2(T) as a member of the genus Bacillus in 16S rRNA-based phylogenetic trees, the misclassification remained uncorrected for many years, which was likely due to the extremely difficult, analysis-hampering cultivation conditions and poor growth rate of the strain. Here we provide a taxonomic re-evaluation of strain T2(T) (= DSM 2912 = NBRC 15312) and propose its reclassification as the type strain of a new species, Kyrpidia tusciae, and the type species of the new genus Kyrpidia, which is a sister-group of Alicyclobacillus. The family Alicyclobacillaceae da ...
18-26S rDNA loci were mapped on chromosomes in four species of Paris, and the number and position of rDNA sites in these species were compared for analysis of the distribution of the sites. All the plants were diploids, and the genome consisted of five chromosomes, A, B, C, D and E. (1) P. polyphylla var. yunnanensis, 2n = 10 = 6m + 4t. Two 18-26S rDNA loci were detected on the short arms of C and D chromosomes; (2) P. forrestii, 2n = 10 = 6m + 4t. One locus was detected on the long arm of B chromosome, and also two loci on the short arms of C and D chromosomes; (3) P. axialis. 2n = 10 = 6m(2sat) + 4t(2sat) + 1 - 2B. Two loci were detected on the short arms of C and D chromosomes. One locus was detected in the cell with two B-chromosomes (B), but none was detected in that with only one B chromosome, indicating that rRNA gene existed on B chromsome, and an unequal division occurred during mitotic cycle of B-chromosomes. (4) P. daliensis, 2n = 10 = 4m + 2sm + 2st + 2t. One locus was detected on ...
Background: In plants, the 5 S rRNA genes usually occur as separate tandems (S-type arrangement) or, less commonly, linked to 35 S rDNA units (L-type). The activity of linked genes remains unknown so far. We studied the homogeneity and expression of 5 S genes in several species from family Asteraceae known to contain linked 35 S-5 S units. Additionally, their methylation status was determined using bisulfite sequencing. Fluorescence in situ hybridization was applied to reveal the sub-nuclear positions of rDNA arrays. Results: We found that homogenization of L-type units went to completion in most (4/6) but not all species. Two species contained major L-type and minor S-type units (termed L-s-type). The linked genes dominate 5 S rDNA expression while the separate tandems do not seem to be expressed. Members of tribe Anthemideae evolved functional variants of the polymerase III promoter in which a residing C-box element differs from the canonical angiosperm motif by as much as 30%. On this basis, ...
We isolated Saccharomyces cerevisiae yeast strains that are able to carry out the second fermentation of sparkling wine from spontaneously fermenting musts in El Penedès (Spain) by specifically designed selection protocols. All of them (26 strains) showed one of two very similar mitochondrial DNA (mtDNA) restriction patterns, whereas their karyotypes differed. These strains showed high rates of karyotype instability, which were dependent on both the medium and the strain, during vegetative growth. In all cases, the mtDNA restriction pattern was conserved in strains kept under the same conditions. Analysis of different repetitive sequences in their genomes suggested that ribosomal DNA repeats play an important role in the changes in size observed in chromosome XII, whereas SUC genes or Ty elements did not show amplification or transposition processes that could be related to rearrangements of the chromosomes showing these sequences. Karyotype changes also occurred in monosporidic diploid ...
Green algae (Chlorophyta) are a morphologically heterogeneous group that is undergoing considerable revisions at present. Especially in coccoid genera, there have been striking cases of polyphyly, when species originally placed in one genus were shown to belong to up to three different classes. The coccoid chlorophycean genus Bracteacoccus Tereg was until recently considered monophyletic, but with the advent of new molecular data, it no longer appears as such. The goal of my project is to monograph the genus Bracteacoccus. I collect 18S ribosomal DNA sequences (nuclear gene) as well as rbcL sequences (chloroplast, protein-coding gene). Phylogeny obtained from the sequence data can be subsequently used as a starting point for further research: well supported clades can be examined for defining traits. Like other coccoid genera, Bracteacoccus has very simple morphology and therefore few characters to be scored. Transmission electron microscopy may provide one or several taxonomically useful ...
Green algae (Chlorophyta) are a morphologically heterogeneous group that is undergoing considerable revisions at present. Especially in coccoid genera, there have been striking cases of polyphyly, when species originally placed in one genus were shown to belong to up to three different classes. The coccoid chlorophycean genus Bracteacoccus Tereg was until recently considered monophyletic, but with the advent of new molecular data, it no longer appears as such. The goal of my project is to monograph the genus Bracteacoccus. I collect 18S ribosomal DNA sequences (nuclear gene) as well as rbcL sequences (chloroplast, protein-coding gene). Phylogeny obtained from the sequence data can be subsequently used as a starting point for further research: well supported clades can be examined for defining traits. Like other coccoid genera, Bracteacoccus has very simple morphology and therefore few characters to be scored. Transmission electron microscopy may provide one or several taxonomically useful ...
Changes in the structure and composition of a protistan community were characterized through the analysis of small-subunit ribosomal RNA gene (18S) sequences for a 3-day bottle incubation using a single sample collected in the western North Atlantic. Cloning and sequencing was used to investigate changes in perceived species richness and diversity as a consequence of environmental perturbation. The treatments included a control (unamended seawater), inorganic nutrient enrichment, and enrichment with a complex organic mixture. Five clone libraries were constructed and analyzed at the time of collection (t-0 h) and after 24 (t-24 h) and 72 (t-72 h) h for the control, and at t-72 h for the inorganic and organic enrichments, resulting in an analysis of 1,626 partial 18S rDNA sequences that clustered into 238 operational taxonomic units (OTUs). Analysis of the clone libraries revealed that protistan assemblages were highly dynamic and changed substantially at both the OTU level and higher taxonomic ...
The higher proliferation rate of cancer cells requires an increased rate of protein synthesis. Thus, cancer cells often show increased rates of ribosomal DNA (rDNA) transcription and have more ribosomes and larger nucleoli, which are nuclear structures that function in ribosome biogenesis. Neumüller et al. identified genes in yeast that, when ablated, resulted in smaller or larger nucleoli. A similar analysis in Drosophila enabled the identification of evolutionarily conserved molecular complexes that increase or decrease nucleolar size when the complex constituents were targeted by RNA interference. Understanding how cells regulate rDNA transcription could provide new therapeutic avenues for interfering with the unrestricted growth that occurs in cancer.. ...
Under the intluence of 5-tluoro-uridine, the ultrastructure of the rDNA transcription units in Xenopus oocytes is altered. Whereas part of the matrix units maintains anormal aspect or shows various degrees of inhibition, in a strong proportion of the transcription units the alternating pattern of matrix units and fibril-free spacer regions is no longer recognized. Transcriptional complexes are found along the entire DNP axis, including the regions of the spacers. These observations support biochemical data on transcription in rDNA spacer region ...
H1S186ph. H1.4 serine 186 (reported as H1S187) phosphorylation is preferentially associated with active rDNA promoters and enriched at hormone response element (PMID: 20439994). ...
Sulfitobacter sp. ATCC ® BAA-1142D™ Designation: Genomic DNA from EE-36 TypeStrain=False Application: Genome sequencing strain
Thermobaculum terrenum ATCC ® BAA-798D-5™ Designation: Genomic DNA from Thermobaculum terrenum strain YNP1 (ATCC ® BAA-798™) TypeStrain=False Application:
TCDRVDCK (*) OWC Drive Dock with USB-C (USB 3.1 Gen 2) Dual Drive Bay Solution. Add up to two 2.5" and 3.5" SATA drives. Connect via USB 3.1 Gen 2 (up to 10Gb/s). Internal power with independent power switches. *New, Open Box*
As discussed in this http://www.daz3d.com/forums/discussion/58379/v4-uvs-for-g3f/ thread. Heres my method for converting textures to Gen3
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... FIGURA 4-3 Vias de sinalização e funções do TLRs. Os TLRs 1, 2, 5 e 6 utilizam a proteína adaptadora MyD88 e
Want to know more about Aluvia Side Effects? started aluvia+colamziv a month ago ,iam pregnant and everything seemed fine lately m | Wed, 23 Mar 2011 |
Obtaining full-length 16S rRNA gene sequences is important for generating accurate taxonomy assignments of bacteria, which normally is realized via clone library construction. However, the application of clone library has been hindered due to its limitations in sample throughput and in capturing minor populations (<1 % of total microorganisms). To overcome these limitations, a new strategy, two-step denaturing gradient gel electrophoresis (2S-DGGE), is developed to obtain full-length 16S rRNA gene sequences. 2S-DGGE can compare microbial communities based on its first-round DGGE profiles and generate partial 16S rRNA gene sequences (8-534 bp, Escherichia coli numbering). Then, strain-specific primers can be designed based on sequence information of bacteria of interest to PCR amplify their remaining 16S rRNA gene sequences (515-1541 bps, E. coli numbering). The second-round DGGE can confirm DNA sequence purity of these PCR products. Finally, the full-length 16S rRNA gene sequences can be ...
A Gram-stain-positive, aerobic, non-spore-forming, atrichous and short rod-shaped endophytic actinomycete, designated strain BGMRC 2075 , was isolated from the leaves of Kandelia candel, and was subjected to polyphasic characterization to unravel its taxonomic position. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BGMRC 2075 belongs to the genus Nocardioides ,showing the highest 16S rRNA gene sequence similarity to Nocardioides aestuarii JC2056 (96.1 %), Nocardioides agariphilus MSL-28 (95.1 %) andNocardioides islandiensis MSL-26 (95.1 %). The predominant cellular fatty acids of strain BGMRC 2075 were iso-C16 : 0, C17 : 1ω8c and C17 : 0. The major menaquinone was MK-8(H4). The diagnostic diamino acid in the cell-wall peptidoglycan was ll-2,6-diaminopimelic acid. The predominant cell-wall sugars were composed of ribose and glucose. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, ...
Strains VIM M 10366(T), YIM M 10378(T) and YIM M 10400(T) were isolated from marine sediments collected from the Xisha Islands in the South China Sea. All three isolates were able to grow optimally at pH 7.0, 28-37 degrees C and 0-3% (w/v) NaCl. Comparison of 16S rRNA gene sequences showed that these strains are members of the genus Streptomyces, exhibiting moderately high 16S rRNA gene sequence similarities of 97.0-98.8% to members of the most closely related Streptomyces species. Morphological characteristics, physiological characteristics and compositions of whole-cell sugars and phospholipids are consistent with the diagnostic characteristics of the genus Streptomyces, but still allowed differentiation amongst the three strains and their neighbours. Based on 16S rRNA gene sequence analysis, DNA DNA relatedness, phenotypic characteristics and chemotaxonomic data, strains VIM M 10366(T), VIM M 10378(T) and VIM M 10400(T) were identified as members of three novel species of the genus ...
A Gram-staining-positive, cocci, halotolerant bacterial strain, designated as SV-16T, was isolated from marine sediment and subjected to a polyphasic taxonomic study. The strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Salinicoccus. Growth occurs at temperatures in the range between 25-37 °C (optimum 30 °C), pH 7.0-11.0 (optimum 8.0) and at NaCl concentrations up to 25 .0 % (optimum 15.0 %). The highest level of 16S rRNA gene sequence similarity was with Salinicoccus carnicancri CrmT (98.6 %) followed by Salinicoccus halodurans W24T (96.6 %). The predominant polar lipids are diphosphatidylglycerol (DPG), phosphatidylinositol (PI) and phosphatidylglycerol (PG). The major cellular fatty acids are iso-C15: 0, anteiso-C15: 0, iso-C17: 0 and anteiso C17: 0. The draft genome of strain SV-16T consisted of 2,591,284 bp with G+C content of 48.7 mol %. On the basis of the phenotypic characteristics and genotypic ...
INTRODUCTION. The genus Bacillus is a phenotypically large, diverse collection of Gram-positive or Gram-variable staining, endospore-forming, aerobic or facultatively anaerobic, rod-shaped bacteria that have undergone considerable reclassification as advances in molecular biology have revealed a high phylogenetic heterogeneity (5, 21). The genus Bacillus and related genera are distributed widely in nature and include thermophilic, psychrophilic, acidophilic, alkalophilic and halophilic bacteria that utilize a wide range of carbon sources for heterotrophic growth or grow autotrophically.. The investigations on phylogenetic divergence of the genus Bacillus and its mesophilic and thermophilic members indicated the need for further and extensive studies to place some of these bacilli in appropriate taxonomic levels (1, 23, 21). With the accumulation of further 16S rRNA gene sequence data, Bacillus has been divided into more manageable and better-defined groups (16). According to Ludwig et al. (2007) ...
In Egypt, four species of Tilapia have been described based on morphometric, meristic and cytotaxonomical characteristics. These species are Tilapia zillii, Oreochromis niloticus, Oreochromis aureus and Sarotherodon galilaeus. The accurate identification of these fishes is complicated by the high variation in these characters,similarity among species and in some cases by the size of the fish. In this paper, we examined the use of polymerase chain reaction (PCR)and restriction fragment length polymorphisms (RFLPs) analysis of the nuclear small subunit ribosomal RNA gene (srDNA) for molecular identification of Tilapia spp. in Egypt. The present study aims to evaluate such advanced molecular biological approach for identification of Tilapia spp. Genomic DNA was extracted from the four species of Tilapia. About 2000 bp 18S ribosomal DNA was amplified by PCR using specific primers. The technique of restriction fragment length polymorphisms was used to identify the specific 18S rDNA for each species. O.
article{7225551, abstract = {A Gram-stain-positive, ovoid, lactic acid bacterium, strain LMG 27676(T), was isolated from a spoiled sous-vide-cooked rutabaga. 16S rRNA gene sequence analysis indicated that the novel strain belongs to the genus Leuconostoc, with Leuconostoc kimchii and Leuconostoc miyukkimchii as the nearest neighbours (99.1 and 98.8\% 16S rRNA gene sequence similarity towards the type strain, respectively). Phylogenetic analysis of the 16S rRNA gene, multilocus sequence analysis of the pheS, rpoA and atpA genes, and biochemical and genotypic characteristics allowed differentiation of strain LMG 27676(T) from all established species of the genus Leuconostoc. Strain LMG 27676(T) (=R-50029(T)=MHB 277(T)=DSM 27776(T)) therefore represents the type strain of a novel species, for which the name Leuconostoc rapi sp. nov. is proposed.}, author = {Lyhs, Ulrike and Snauwaert, Isabel and Pihlajaviita, Seija and De Vuyst, Luc and Vandamme, Peter}, issn = {1466-5026}, journal = {INTERNATIONAL ...
Background. 16S rRNA gene sequences are routinely assigned to operational taxonomic units (OTUs) that are then used to analyze complex microbial communities. A number of methods have been employed to carry out the assignment of 16S rRNA gene sequences to OTUs leading to confusion over which method is the most rigorous. A recent study suggested that a clustering method should be selected based on its ability to generate stable OTU assignments that do not change as additional sequences are added to the dataset. In contrast, we contend that the ability of the method to properly represent the distances between the sequences is more important. Methods. Our analysis implemented five de novo clustering algorithms including the single linkage, complete linkage, average linkage, abundance-based greedy clustering, distance-based greedy clustering, and the open and closed-reference methods. Using two previously published datasets we used the Matthews Correlation Coefficient (MCC) to assess the stability and
Looking for online definition of DNA-DNA Reassociation in the Medical Dictionary? DNA-DNA Reassociation explanation free. What is DNA-DNA Reassociation? Meaning of DNA-DNA Reassociation medical term. What does DNA-DNA Reassociation mean?
Gammaproteobacteria belonging and related to the genus Microbulbifer are an emerging group of complex carbohydrate-degrading marine bacteria. Previously, all of the representatives were placed within Microbulbifer or were unclassified. Recently, a new genus, Teredinibacter, represented by a single species, Teredinibacter turnerae, was formed to include an endosymbiotic branch of these organisms. In this study, based on 16S rRNA gene sequence similarity and phenotypic analyses, a new genus, Saccharophagus, is proposed to accommodate the most versatile marine carbohydrate degrader yet identified, Saccharophagus degradans gen. nov., sp. nov. 2-40(T) (=ATCC 43961(T)=DSM 17024(T)). S. degradans strain 2-40(T) can degrade 10 tested complex polysaccharides: agar, alginate, chitin, cellulose, fucoidan, laminarin, pectin, pullulan, starch and xylan. S. degradans 2-40(T) shares 90.5% 16S rRNA gene sequence similarity with the type strain of the Microbulbifer type species, Microbulbifer hydrolyticus ...
Among multigene families, ribosomal RNA (rRNA) genes are the most frequently studied and have been explored as cytogenetic markers to study the evolutionary history of karyotypes among animals and plants. In this report, we applied cytogenetic and genomic methods to investigate the organization of rRNA genes among cichlid fishes. Cichlids are a group of fishes that are of increasing scientific interest due to their rapid and convergent adaptive radiation, which has led to extensive ecological diversity. The present paper reports the cytogenetic mapping of the 5S rRNA genes from 18 South American, 22 African and one Asian species and the 18S rRNA genes from 3 African species. The data obtained were comparatively analyzed with previously published information related to the mapping of rRNA genes in cichlids. The number of 5S rRNA clusters per diploid genome ranged from 2 to 15, with the most common pattern being the presence of 2 chromosomes bearing a 5S rDNA cluster. Regarding 18S rDNA mapping, the
Ribosomal DNA (rDNA) of plants is present in high copy number and shows variation between and within species in the length of the intergenic spacer (IGS). The 45S rDNA of flowering plants includes the 5.8S, 18S and 25S rDNA genes, the internal transcribed spacer (ITS1 and ITS2), and the intergenic spacer 45S-IGS (25S-18S). This study identified six different types of 45S-IGS, A to F, which at 363 bp, 1121 bp, 1717 bp, 1969 bp, 2036 bp and 2111 bp in length, respectively, were much shorter than the reported reference IGS sequences in B. oleracea var. alboglabra. The shortest two IGS types, A and B, lacked the transcription initiation site, non-transcribed spacer, and external transcribed spacer. Functional behavior of those two IGS types in relation to rRNA synthesis is a subject of further investigation. The other four IGSs had subtle variations in the transcription termination site, guanine-cytosine (GC) content, and number of tandem repeats, but the external transcribed spacers of these four IGSs were
An obligately aerobic, chemoheterotrophic, mesophilic prosthecate bacterium, designated strain CGM1-3ENT, was isolated from the enrichment cultures of forest soil from Cheonggyesan Mountain, Republic of Korea. Cells were Gram-reaction-negative, motile rods (1.3–2.4 µm long by 0.30–0.75 µm wide) with single flagella. The strain grew at 10–37 °C (optimum 25–30 °C) and at pH 4.5–9.5 (optimum 5.0–7.0). The major cellular fatty acids were C16 : 0, C18 : 1ω7c 11-methyl, C12 : 1 3-OH and summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c). The genomic DNA G+C content of strain CGM1-3ENT was 63.7 mol%. The closest phylogenetic neighbour to strain CGM1-3ENT was identified as Asticcacaulis biprosthecium DSM 4723T (97.2 % 16S rRNA gene sequence similarity) and the DNA–DNA hybridization value between strain CGM1-3ENT and A. biprosthecium DSM 4723T was less than 24.5 %. Strain ...
A Gram-positive, rod-shaped, endospore-forming organism, strain BL3-6(T), was isolated from tidal flat sediments of the Yellow Sea in the region of Tae-An. A 16S rRNA gene sequence analysis demonstrated that this isolate belongs to the Bacillus cereu
Abstract. Mealybugs (Hemiptera: Pseudococcidae) are small, plant-sucking insects which comprise the second largest family of scale insects (Coccoidea). Relationships among many pseudococcid genera are poorly known and there is no stable higher level classification. Here we review previous hypotheses on relationships and classification and present the first comprehensive phylogenetic study of the Pseudococcidae based on analysis of nucleotide sequence data. We used three nuclear genes, comprising two noncontiguous fragments of elongation factor 1α (EF-1α 5′ and EF-1α 3′), fragments of the D2 and D10 expansion regions of the large subunit ribosomal DNA gene (28S), and a region of the small subunit ribosomal DNA gene (18S). We sampled sixty-four species of mealybug belonging to thirty-five genera and representing each of the five subfamilies which had been recognized previously, and included four species of Puto (Putoidae) and one species each of Aclerda (Aclerdidae) and Icerya ...
... , abstract = {Phylogenetic relationships of resupinate Homobasidiomycetes (Corticiaceae s. lat. and others) were studied using ribosomal DNA (rDNA) sequences from a broad sample of resupinate and nonresupinate taxa. Two datasets were analysed using parsimony, acoredataset of 142 species, each of which is represented by four rDNA regions (mitochondrial and nuclear large and small subunits), and a full clataset of 656 species, most of which were represented only by nuclear large subunit rDNA sequences. Both datasets were analysed using traditional heuristic methods with bootstrapping, and the full clataset was also analysed with the Parsimony Ratchet, using equal character weights and six-parameter weighted parsimony. Analyses of both datasets supported monophyly of the eight major clades of Homobasicliomycetes recognised by Hibbett and Thorn, as well as independent lineages corresponding to the Gloeophyllum clade, corticioid clade and jaapia argillacea. Analyses ...
An obligately anaerobic bacterial strain designated T-1-35(T) was isolated as a dominant cultivable cellulose-degrading bacterium from soil of a Japanese rice field as an anaerobic filter-paper degrader. Cells of strain T-1-35(T) stained Gram-positive and were non-spore-forming rods with rounded ends, 0.8-1.0 3.5-15.0 m, and motile by means of two to four polar flagella. Cells of strain T-1-35(T) exhibited pleomorphism: in aged cultures (over 90 days of incubation), almost all cells were irregularly shaped. Although no spore formation was observed, cells tolerated high temperatures, up to 90 C for 10 min. The temperature range for growth was 15-40 C, with an optimum at 35 C. The pH range for growth was 5.5-9.0, with an optimum at pH 8.0-8.5 (slightly alkaliphilic). Strain T-1-35(T) fermented some carbohydrates to produce ethanol and lactate as the major products. Major cellular fatty acids were iso-C16 : 0 and iso-C13 : 0 3-OH. Phylogenetic analysis based on the 16S rRNA gene sequence revealed ...
A novel Gram-positive, aerobic, actinobacterial strain, CF6/1T, was isolated in 2007 during environmental screening of arid desert soil in the Sahara near to Ourba, Chad. The isolate was found to grow best in a temperature range of 20-37 °C and at pH 6.0-8.5 and showed no NaCl tolerance, forming black-coloured and nearly circular colonies on GYM agar. Chemotaxonomic and molecular characteristics determined for the isolate match those previously described for members of the genus Geodermatophilus. The DNA G + C content of the novel strain was determined to be 74.9 mol %. The peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid. The main phospholipids were determined to be phosphatidylethanolamine, phosphatidylinositol, phosphatidylcholine, diphosphatidylglycerol and traces of phosphatidylglycerol; MK-9(H4) was identified as the dominant menaquinone and galactose as the diagnostic sugar. The major cellular fatty acids were found to be the branched-chain ...
Antibodies for proteins involved in negative regulation of transcription elongation from RNA polymerase I promoter pathways, according to their Panther/Gene Ontology Classification
This is the first evaluation of a novel fast broad-range 16S rDNA PCR/sequencing assay in a Canadian patient population, and the first study of the clinical utility of DPO primers for the routine molecular analysis of heart valve tissue in consecutive patients with and without infective IE. Additionally, our study had the advantage of extensive clinical information to better delineate the Dukes minor criteria for all patients. Our study confirms the higher sensitivity of molecular heart valve testing compared to tissue culture [35-38]. However, our novel assay has one of the highest reported sensitivities of a user-developed broad-range 16S rDNA PCR to date. Although pre-operative blood cultures make a microbiologic diagnosis in approximately two-thirds of patients suspected of having IE, subsequent molecular analysis of heart valve tissue contributed to the microbiologic diagnosis of 31 % of our patients. However, the change in diagnosis would only be expected to contribute to the clinical ...
Read "Genetic Variation of Nuclear Ribosomal DNA in Cockroaches (Order Blattaria): Phylogenetic Analysis of Restriction Fragment Length Polymorphism, Russian Journal of Genetics" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
The family Mactridae is composed of a diverse group of marine organisms, commonly known as trough shells or surf clams, which illustrate a global distribution. Although this family includes some of the most fished and cultured bivalve species, their chromosomes are poorly studied. In this work, we analyzed the chromosomes of Spisula solida, Spisula subtruncata and Mactra stultorum by means of fluorochrome staining, C-banding and fluorescent in situ hybridization using 28S ribosomal DNA (rDNA), 5S rDNA, H3 histone gene and telomeric probes. All three trough shells presented 2n = 38 chromosomes but different karyotype compositions. As happens in most bivalves, GC-rich regions were limited to the nucleolus organizing regions in Spisula solida. In contrast, many GC-rich heterochromatic bands were detected in both Spisula subtruncata and Mactra stultorum. Although the three trough shells presented single 5S rDNA and H3 histone gene clusters, their chromosomal locations differed. Regarding major rDNA clusters
Aime, M. C., P. B. Matheny, D. A. Henk, E. M. Frieders, R. H. Nilsson, D. J. McLaughlin, L. J. Szabo, and D. S. Hibbett. 2006. An overview of the higher-level classification of Pucciniomycotina based on combined analyses of nuclear large and small subunit rDNA sequences. Mycologia 98: 869-905.. Alexopoulos, C.J., Mims, C.W. and Blackwell, M. 1996. Introductory Mycology. John Wiley and Sons, New York.. Arora, D. 1986. Mushrooms Demystified. Ten Speed Press, Berkeley, California.. Bauer, R., Begerow, D., Oberwinkler, F., Piepenbring, M. and Berbee, M. L 2001. Ustilaginomycetes. Pp. 57-84. In: The Mycota VII. Systematics and Evolution. Part B. (Mclaughlin, D. J., McLaughlin, E. G. and Lemke, P. A., eds.). Springer-Verlag, Berlin.. Begerow D, Stoll M, and R. Bauer. 2006. A phylogenetic hypothesis of Ustilaginomycotina based on multiple gene analyses and morphological data. Mycologia 98: 906 916.. Benjamin, D.R. 1995. Mushrooms: poisons and panaceas. W.H. Freeman and Company, New York.. Buller, ...
We have initiated comparative studies of ribosomal RNA (rRNA) gene structure to explore its potential to provide taxonomically useful data within the large red algal order Gigartinales. In southern Australia, this group is extremely diverse and includes large numbers of endemic taxa, many of potential economic importance. The 5.8S rRNA gene occurs in the middle region of the ribosomal DNA (rDNA) cistron and is flanked by two internal transcribed spacers (ITSs). These spacers contain regions of DNA, which are highly consented at the generic level and above, interspersed with highly divergent sequences. The 5.8S and associated ITS s of 11 species of Gigartinales (including five species of the largest Australian endemic marine algal genus, Mychodea), plus five taxa belonging to other orders, were amplified by the polymerase chain reaction. The size of the 5.8S rDNA and its flanking ITSs varied not only within and between genera, but also at the species level. However, this rDNA sequence appears to be
Looking for Amoebobacter? Find out information about Amoebobacter. A genus of bacteria in the family Chromatiaceae; cells are spherical and nonmotile, have gas vacuoles, and contain bacteriochlorophyll a on vesicular... Explanation of Amoebobacter
The knowledge of cytogenetics in the harvestmen family Phalangiidae has been based on taxa from the Northern Hemisphere. We performed cytogenetic analysis on Guruia africana (Karsch, 1878) (2n=24) and four species of the genus Rhampsinitus Simon, 1879 (2n=24, 26, 34) from South Africa. Fluorescence in situ hybridization with an 18S rDNA probe was used to analyze the number and the distribution of this cluster in the family Phalangiidae for the first time. The results support the cytogenetic characteristics typical for the majority of harvestmen taxa, i.e. the predominance of small biarmed chromosomes and the absence of morphologically well-differentiated sex chromosomes as an ancestral state. We identified the number of 18S rDNA sites ranging from two in R. qachasneki Kauri, 1962 to seven in one population of R. leighi Pocock, 1903. Moreover, we found differences in the number and localization of 18S rDNA sites in R. leighi between populations from two localities and between sexes of R. capensis (Loman,
Drosophila mercatorum is a bisexual species, but certain strains are capable of parthenogenetic reproduction in the laboratory. We investigated the parthenogenetic capacity of the virgin daughters of females captured from a natural, bisexual population in Hawaii. An isozyme survey indicated the natural population is polymorphic at about 50% of its loci, and its individuals heterozygous at 18% of their loci. The predominant mode of parthenogenesis in D. mercatorum causes homozygosity for all loci in a single generation. Despite this radical change in genetic state, 23% of the virgin female lines produced adult parthenogenetic progeny, and 16% produced parthenogenetic progeny themselves capable of parthenogenetic reproduction. The parthenogenetic rate as measured by the number of parthenogenetic progeny themselves capable of parthenogenesis divided by the number of eggs laid is around 10-5 for the virgin female lines. We argue that one of the major reasons for this low rate is that very few of the ...
The resolution of the OFRG analysis was evaluated by examining the nucleotide sequences of the clones in six clusters distributed throughout the UPGMA tree (Fig. 3A, clusters 1 and 4 to 8). Clusters 2 and 3 (Fig. 3A) were not used for this analysis because only two clones from these groups were fully sequenced. For each cluster, pairwise sequence analysis showed that clones with an average sequence identity of 97% were grouped in the same cluster. The range of identities was 92.7 to 100%, and 79% of the values were between 96 and 98%. Thus, this OFRG analysis approximated species level resolution because DNA-DNA reassociation experiments have been used to show that bacterial rDNA with sequence identities of 97% or more are likely to come from the same species (25). In general, the resolution of OFRG depends on probe set properties, such as the number of probes, their discriminatory power, and the suitability of the set for a specific environment. Further refinements of probe selection algorithms ...
Complete information for RRN3 gene (Protein Coding), RRN3 Homolog, RNA Polymerase I Transcription Factor, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The five most frequent keywords within the labels of environmental samples which yielded hits were microbi (9.4%), hypersalin (9.1%), http://www.selleckchem.com/products/pazopanib.html mat (8.6%), len, miniprim, new, view, world (8.5%) and food (3.4%). The single most frequent keyword within the labels of environmental samples which yielded hits of a higher score than the highest scoring species was hypersalin, len, mat, microbi, miniprim, new, view, world (12.5%). These key words are in line with the ecology and the niche from where strains of H. praevalens have been isolated. Figure 1 shows the phylogenetic neighborhood of H. praevalens GSLT in a 16S rRNA gene based tree.. The sequences of the four 16S rRNA gene copies in the genome differ from each other by up to five nucleotides, and differ by up to five nucleotides from the previously published 16S rRNA gene sequence ("type":"entrez-nucleotide","attrs":"text":"AB022034″,"term_id":"4127263″,"term_text":"AB022034″AB022034). ...
... : Phylogram (neighbor-joining method) showing genetic relationship between strain ETL-1982 and other microorganisms based on the 16S rRNA gene sequence analysis ...
Bacterial community composition, as revealed by deep 16S sequence analyses, is argued to contribute to diverse human health and disease states (10). While the microbial community structure has been shown to influence susceptibility to infection in models of gastrointestinal disease (2, 4, 5, 34), the application of this concept to the female urinary tract has not been pursued. To define the existence and compositions of bladder bacterial communities in human females without the confounding factor of possible vulvo-vaginal contamination, we carefully sampled urine directly from female bladders using TUC and SPA. Deep 16S rRNA gene sequencing of these samples revealed that bacterial bladder communities of different types do exist in women, although not in all individuals. These data confirm and extend results of earlier studies (17, 21-23), clearly showing that urine specimens reported to clinicians as "culture-negative" or "insignificant growth" can contain varied bacterial communities that can ...
Cavalcanti, Sarah Desir e Barbosa et al. Analysis of the genetic polymorphism of Paracoccidioides brasiliensis and Paracoccidioides cerebriformis "Moore" by random amplified polymorphic DNA (RAPD) and 28S ribosomal DNA sequencing: Paracoccidioides cerebriformis revisited. Rev. Inst. Med. trop. S. Paulo, June 2005, vol.47, no.3, p.119-123. ISSN 0036- ...
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16. A method of stabilizing a spine with a spinal stabilization device comprising a rod-shaped implant comprising a continuous one-piece rod-shaped member having a first end and a second end opposite the first end along a longitudinal axis, the rod-shaped member being made from a plastic material exhibiting flexibility, a longitudinal bore provided in the rod-shaped member, a reinforcing rod accommodated in the bore and made from a more rigid material than the material of the rod-shaped member, the reinforcing rod having a first end and a second end, wherein the reinforcing rod is slidable in the bore; and wherein the reinforcing rod comprises a stop contacting the rod-shaped member to limit movement of the reinforcing rod relative to the rod-shaped member, and at least two bone anchoring elements, each bone anchoring element having a receiving part for receiving the rod-shaped member, the method comprising:attaching the first bone anchoring element to a bone or vertebra;attaching the second ...
Kroh, A. (2012). Holasteroida incertae sedis. In: Kroh, A. & Mooi, R. (2017). World Echinoidea Database. Accessed through: World Register of Marine Species at http://marinespecies.org/aphia.php?p=taxdetails&id=510772 on 2018-01- ...
Slopalinida is a heterokont order. David J. Patterson Kostka M, Hampl V, Cepicka I, Flegr J (October 2004). "Phylogenetic position of Protoopalina intestinalis based on SSU rRNA gene sequence". Mol. Phylogenet. Evol. 33 (1): 220-4. doi:10.1016/j.ympev.2004.05.009. PMID 15324850. Patterson, D.J. (1985). "The fine structure of Opalina ranarum (Family Opalinidae): Opalinid phylogeny and classification". Protistologica. 21: 413-428. "www.ncbi.nlm.nih.gov". Retrieved 2009-06-17 ...
View Notes - lab Write-up from ENGLISH 1011 at Berkeley. Jacob Zipperstein H. Bio Med January 28, 2009 Title: DNA Restriction Analysis (Lambda DNA) Purpose: The purpose of this lab is to analyze
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
A method to estimate the ice volume and ice-thickness distribution of alpine glaciers - Volume 55 Issue 191 - Daniel Farinotti, Matthias Huss, Andreas Bauder, Martin Funk, Martin Truffer
The SeqRank project aims are to develop approaches for automatic selection of high-quality molecular sequences for microbial strains, through the use of modern ranking techniques. Currently, the SeqRank algorithm can automate the process of selecting a high-quality 16S rRNA gene sequence for any given prokaryotic strain, based on a series of quality statistics, the latest status of taxonomy and the latest sequence information from the INSDC databases. The extended SeqRank workflow applies SeqRank to all type strains of a given prokaryotic genus or family, adds an automatically chosen outgroup sequence, and infers a phylogenetic tree from the resulting list of sequences.. Contact: [email protected] or the StrainInfo team (see contact). ...
It has been shown that the overall transcription of ribosomal RNA genes can be stimulated by many signals (41); however, increased transcription is not due to an increased number of actively transcribed rDNA units but instead is due to changes in the rate of transcription, especially of elongation (42, 43). B-WICH is an ATP-dependent chromatin remodeling complex containing SNF2h, a human ISWI ATPase, and it was shown to associate with Pol I facilitating its transcription (30). The SIRT7 interaction with components of the B-WICH complex supports a hypothesis where SIRT7 regulates the rate of elongation of Pol I through the ATP-dependent remodeling activities of B-WICH.. SIRT7 knockdown is known to inhibit rDNA transcription (9, 10), and our results show for the first time that SIRT7 knockdown also leads to a reduction in the large subunit of Pol I at the protein level but not at the mRNA level. A question to be addressed in future studies is whether this regulation of Pol I protein level occurs ...
Designation of Streptomycete 16S and 23S rRNA-based target regions for oligonucleotide probes.: The 16S and 23S rRNA of various Streptomyces species were partia
Peptoniphilus asaccharolyticus DSM 20463 strain CCUG 9988 clone 8 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial ...
The internal transcribed spacers (ITS) of the nuclear ribosomal DNA have been sequenced from 29 collections of Phillipsia, mainly from the New World. The P domingensis complex, collections with a range of colors but otherwise referable to P domingensis s.l. based on spore ornamentation, were studied. Three distinctive species of Phillipsia also were included. The sequences were analysed to infer phylogenetic relationships within Phillipnsia, using parsimony. Morphological features were studied separately, and then evaluated in the context of the ITS phylogeny. Four distinct rDNA lineages, supported by ascospore ornamentation, were identified: the P. crispata the P. domingensis, the P. olivacea and the P. carnicolor lineages. SEM photographs of the ascospores are presented. Phillipsia lutea and another yellow form were nested within the P. dominagensis complex, of those with reddish hymenial colors. Color has been emphasized in taxonomy of Phillipsia, but these results suggest that individuals ...
Sequence logo and spacer length distribution of representative signals for the genomes (A) E. coli k-12; (B) S. coelicolor; (C) A. fulgidus; and (C) Synechocyst
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Supplementary table Species, systematic position and accession number of mitochondrial genome sequences used in the phylogenetic analysis and for gene order comparisons
另外,在43株具分解辛基苯酚聚氧乙基醇能力的菌株中,Pseudomonas nitroreducens TX1生長速率最快且對辛基苯酚聚氧乙基醇的耗氧活性最高,並能生長在0.05%到20%的辛基苯酚聚氧乙基醇濃度中,其生長速率在0.34到0.44 hr-1之間。以高效能液相層析質譜儀 (HPLC-MS) 分析菌株TX1分解辛基苯酚聚氧乙基醇的代謝產物,推斷此菌株能減短聚氧乙烯鏈並生成辛基苯酚。本研究發現菌株TX1可以進一步以支鏈型辛基苯酚 (4-t-octylphenol) 為唯一碳源生長。P. nitroreducens TX1為第一株被發現同時具有分解辛基苯酚聚氧乙基醇與支鏈型辛基苯酚能力的菌株。以高效能液相層析質譜儀 (LC-ESI-Q-TOF) 分析此菌株以支鏈型辛基苯酚為唯一碳源生長過程中的代謝產物,發現有中間代謝物辛基鄰苯二酚 (4-t-octylcatechol) 生成。其中偵測到兩個經間位裂解 (meta-cleavage) 途徑產生之代謝物: ...
Here, we present a protocol for tracing genomic DNA (gDNA) contamination in RNA samples. The presented method utilizes primers specific ...
The quality of your garden soil will contribute 50% to the success of your gardening business. Dont overlook its importance. Find out all you need to know
In the interphase cell nucleus, the ribosomal genes are located in the fibrillar centers and in the associated dense fibrillar component of the nucleolus (Hernandez-Verdun, 1983; 1986; Goessens,...
Taxonomy of the species Kitasatospora azatica corrig. (Nakagaito et al. 1993) Zhang et al. 1997 and Kitasatosporia azatica (sic) (Nakagaito et al. 1993) Zhang et al. 1997
Gene sequencing is the process of identifying the base nucleotides in an organisms DNA. Practical applications of gene sequencing...
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Stults JR, Snoeyenbos-West O, Methe B, Lovley DR, Chandler DP. 2001. Application of the 5 fluorogenic exonuclease assay (TaqMan) for quantitative ribosomal DNA and rRNA analysis in sediments.. Appl Environ Microbiol. 67(6):2781-9. ...
List of words make out of Aesculin. All anagrams of Aesculin. Words made after unscrambling Aesculin. Scrabble Points. Puzzle Solver. Word Creation.
COMPARISON OF THE POTENCY OF POLYVALENT ANTIMENINGOCOCCUS SERUM PRODUCED WITH FOUR AND SIX REPRESENTATIVE STRAINS AND THAT PRODUCED WITH SIXTY STRAINS, AS DETERMINED BY THE AGGLUTINATION ...
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Its been a long time since I had a What Genus Are You? post! But, its back and I hope to keep this somewhat a regular thing! Hunting the Archives site for pretty things has become a new fix for junk/dress hunting - and it doesnt cost a penny ...