Email: [email protected] We have conducted a human cDNA sequencing project for identification of unknown human transcripts in the past 8 years. A distinctive point of our project from other cDNA sequencing projects is in that we have focused our sequencing efforts on long cDNA clones (,4 kb), particularly those encoding large proteins in brain. This approach has brought us a unique cDNA resource consisting of large cDNA clones for newly identified human genes, which are known as KIAA cDNAs. The number of KIAA cDNAs has exceeded 2000 this year (the total number of the sequenced nucleotide residues, about 10 Mb) and we have made them publicly available. Because the number of genes encoding large proteins (,100 kDa) is always smaller than 10% of the total number of genes in eukaryotes, the number of human genes encoding large proteins is likely less than 3000. If this estimate is correct, we must have certainly entered endgame of the identification of human transcribed sequences expressed in the ...
Gene profiling was performed using a PCR-based subtractive hybridisation method, called Representation Difference Analysis (RDA), and kidney tissue from congenic versus hypertensive controls for the SHRSPGla. The RDA method itself I have also refined using glycerol-enhanced mini-polyacrylamide gel electrophoresis to optimize the separation of differentially expressed RDA fragments. I have isolated 20 such fragments that are differentially expressed and have been able to positively identify them by BLASTA search against known sequences on Genbank. These include the cytochrome P450 4A3 gene, which has been well documented as differentially expressed between SHR and WKY kidneys, and the low affinity Na-dependent glucose transporter (SGLT2). The expression of SGLT2 mRNA has been shown to be kidney specific and I have shown that it is differentially expressed in the congenic strain of SP.WKYGla2a versus the parental SHRSPGla. Two novel genes have also been mapped onto our target chromosome 2 within ...
We have conducted a human cDNA project to predict protein-coding sequences (CDSs) in large cDNAs (, 4 kb) since 1994, and the number of newly identified genes, known as KIAA genes, already exceeds 2000. The ultimate goal of this project is to clarify the physiological functions of the proteins encoded by KIAA genes. To this end, the project has recently been expanded to include isolation and characterization of mouse KIAA-counterpart genes. We herein present the entire sequences and the chromosome loci of 500 mKIAA cDNA clones and 13 novel cDNA clones that were incidentally identified during this project. The average size of the 513 cDNA sequences reached 4.3 kb and that of the deduced amino acid sequences from these cDNAs was 816 amino acid residues. By comparison of the predicted CDSs between mouse and human KIAAs, 12 mKIAA cDNA clones were assumed to be differently spliced isoforms of the human cDNA clones. The comparison of mouse and human sequences also revealed that four pairs of human ...
www.MOLUNA.de Differential Display Methods and Protocols [4221492] - Part I. MethodologiesnGlobal Analysis of Gene Expression by Differential Display: A Mathematical ModelnShitao Yang and Peng LiangnAutomation of Fluorescent Differential Display With Digital ReadoutnJonathan D. Meade, Yong-jig Cho, Jeffrey S. Fisher, Jamie C. Walden, Zhen Guo, and Peng LiangnOrdered Differential DisplaynMikhail V. Matz and Ella A. MeleshkevitchnGeneCalling®: Transcript Profiling Coupled
Synthesize full-length cDNA from ng of total RNA. Easy and efficient process maintains true gene representation. cDNA is immediately ready for downstream applications such as PCR, cloning, library construction & subtractive hybridization.
Synthesize full-length cDNA from ng of total RNA. Easy and efficient process maintains true gene representation. cDNA is immediately ready for downstream applications such as PCR, cloning, library construction & subtractive hybridization.
Verification result of the assembled full-length cDNA. Lane M represents the DL 2000 DNA size marker (Takara). Lane 1 represents the overlapping 3 end cDNA a
Definition of complementary DNA - synthetic DNA in which the sequence of bases is complementary to that of a given example of DNA.
有许多人说克隆是一件痛苦的工作,或许,有更多人的感觉使用Takara-Clontech产品进行克隆是一种愉悦。无论您是利用Takara经典的pMD系列进行传统克隆方法,还是使用In-fusion进行的新一代克隆操作;无论您是使用Takara经典的PrimeScript进行cDNA合成,还是使用SMARTer技术进行的新一代cDNA合成操作,Takara-Clontech带给您的都将是舒心、欣喜、兴奋 ...
有许多人说克隆是一件痛苦的工作,或许,有更多人的感觉使用Takara-Clontech产品进行克隆是一种愉悦。无论您是利用Takara经典的pMD系列进行传统克隆方法,还是使用In-fusion进行的新一代克隆操作;无论您是使用Takara经典的PrimeScript进行cDNA合成,还是使用SMARTer技术进行的新一代cDNA合成操作,Takara-Clontech带给您的都将是舒心、欣喜、兴奋 ...
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.. In our series of projects for accumulating sequence information on the coding sequences of unidentified human genes, we have newly determined the sequences of 100 cDNA clones from a set of size-fractionated human brain cDNA libraries, and predicted the coding sequences of the corresponding genes, named KIAA0711 to KIAA0810. These cDNA clones were selected according to their coding potentials of large proteins (50 kDa and more) in vitro. The average sizes of the inserts and corresponding open reading frames were 4.3 kb and 2.6 kb (869 amino acid residues), respectively. Sequence analyses against the public databases indicated that the predicted coding sequences of 78 genes were similar to those of known genes, 64% of which (50 genes) were categorized as proteins functionally related to cell signaling/communication, cell structure/motility and nucleic acid management. As additional information ...
Creative Biogene is a biotechnology company which has the expertise and experience to provide you with high quality subtractive cDNA library construction services.
Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5 ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins ...
EN] We have developed an integrated method to generate a normalized cDNA collection enriched in full-length and rare transcripts from citrus, using different species and multiple tissues and developmental stages. Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. In this regard, the availability of full-length cDNA clones facilitates functional analysis of the corresponding genes enabling manipulation of their expression and the generation of a variety of tagged versions of the native protein. The development of full-length cDNA sequences has the power to improve the quality of genome annotation, as well as provide tools for functional characterization of ...
Identification of cellular inhibitors in opposition to Chikungunya virus replication by a cDNA expression cloning combined with MinION sequencing cDNA expression cloning has been confirmed to be a robust technique inside the search for cellular parts that administration virus replication. On this study, cDNA library screening using a pool of cDNA derived from interferon-treated human […]. ...
Since KIAA genes were characterized as unknown genes at least at the time of being sequenced, little is known about their biological functions. To understand physiological roles of KIAA genes in human, we expect that functional characterization using a model animal makes a great contribution of exploration of physiological functions. In this respect, we selected mouse as the model mammal, and started cloning cDNAs for mouse homologues of KIAA genes ...
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Nagase T، Ishikawa K، Nakajima D، Ohira M، Seki N، Miyajima N، Tanaka A، Kotani H، Nomura N، Ohara O (Apr 1997). Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Research. 4 (2): 141-50. PMID 9205841. doi:10.1093/dnares/4.2.141. ...
Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Aug 1998). Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5 (1): 31-9. doi:10.1093/dnares/5.1.31. PMID 9628581 ...
J:60127 Pascolo S, Tsoukatou D, Mamalaki C, Identification of thymus specific and developmentally regulated genes by an improved version of the mRNA differential display technique. Dev Immunol. 1999;7(1):1-7 ...
NEB offers several reagents for cDNA synthesis upstream of applications such as qPCR and qRT-PCR, including reverse transcriptase.
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Our RT-qPCR kits demonstrate high specificity due to a novel hot-start mechanism, allowing you to detect target sequences down to one copy
The cDNA template used to generate the probe was obtained by PCR as described in the paper. AM12-ORF-R: 5′-ATGTCAAA-GCGTCTCCTTGCG-3′ and AM12-ORF-L: 5′-ATCAT-TTCACATCAGCAGTAGC-3′. As the second primer could not be identified, the sequence shown is NM_001142850.1 ...
Fingerprint Dive into the research topics of Cloning and characterization of a mouse brain calcitonin receptor complementary deoxyribonucleic acid and mapping of the calcitonin receptor gene. Together they form a unique fingerprint. ...
P-57. Generating and Sequencing Full-length cDNAs of Novel Human Genes Within the German cDNA Consortium. Ruth E. Wellenreuther, Stefan Wiemann, Daniela Heiss, Nina Claudino, Annemarie Poustka, Department of Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Heidelberg, GERMANY. We generate human cDNA libraries that are enriched in full length clones i.e. from the translation start to the poly A tail. These libraries are used for a) systematic sequencing within the cDNA consortium of the Genome Project aiming at the identification and analysis of as many new genes as possible and b) for screening to isolate full length clones of partial genes. Libraries are created by directional cloning of cDNAs into plasmid vectors. Full-length enrichment is achieved via Clontechs SMART technology. This method is PCR-based, and in our modified strategy, we amplify and clone selective size windows of the cDNA fraction above 3 kb.Clones from the libraries generated within this project are ...
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The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this area is still limited. Several lines of investigation have been used to elucidate the structure and function of the genes in the human genome. Even so, gene prediction remains a difficult task, as the varieties of transcripts of a gene may vary to a great extent. We thus performed an exhaustive integrative characterization of 41,118 full-length cDNAs that capture the gene transcripts as complete functional cassettes, providing an unequivocal report of structural and functional diversity at the gene level. Our international collaboration has validated 21,037 human gene candidates by analysis of high-quality full-length cDNA clones through curation using unified criteria. This led to the identification of 5,155 new gene candidates. It also manifested the most reliable way to control the quality of the ...
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Information about approximately 38,000 full-length cDNA clones that were completely sequenced in the Rice full-length cDNA project is shown in the database. The full-length cDNA clones were collected from various tissues treated under various stress conditions. The database contains not only information about complete nucleotide sequences and encoded amino acid sequences, but also results of homology searches against public databases, mapping information, information about patterns of alternative splicing, protein domains and transmembrane structures, and information about cellular localizations and gene functions annotated with Gene Ontology.. ...
4 Genome Science Laboratory, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan. E-mail: [email protected] Functional annotation of DNA sequences is recognized as one of the most important processes in genome analysis. In this poster, we present an analysis of detecting functional motifs from the mouse full-length cDNA data, which are being sequenced by RIKEN. Our method detects functional motifs as commonly conserved regions between given cDNA sequences. Detection of such regions may give useful information for the functional assignment of cDNA sequences if the regions can be related with specific functions.. Our method mainly consists of the following two steps, both of which are based on the graph theory.. (1) Clustering of sequences: First, we compute all pairwise similarities of given protein sequences (which are translated from full-length cDNA sequences), and construct a linkage graph. In the graph, a vertex corresponds to a protein, and an edge is weighted by the ...
A large number of cDNA inserts were sequenced from a high-quality library of chicken bursal lymphocyte cDNAs. Comparisons to public gene databases indicate that the cDNA collection represents more than 2,000 new, full-length transcripts. This resource defines the structure and the coding potential o …
Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro ...
Histone H2B type 2-F is a protein that in humans is encoded by the HIST2H2BF gene. GRCh38: Ensembl release 89: ENSG00000203814 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000105827 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Entrez Gene: HIST2H2BF histone cluster 2, H2bf. Strausberg RL, Feingold EA, Grouse LH, et al. (2003). Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-903. doi:10.1073/pnas.242603899. PMC 139241 . PMID 12477932. Cheung WL, Ajiro K, Samejima K, et al. (2003). Apoptotic phosphorylation of histone H2B is mediated by mammalian sterile twenty kinase. Cell. 113 (4): 507-17. doi:10.1016/S0092-8674(03)00355-6. PMID 12757711. Gerhard DS, Wagner L, Feingold EA, et al. (2004). The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14 (10B): 2121-7. doi:10.1101/gr.2596504. PMC ...
The Universal RiboClone™ cDNA Synthesis System contains the reagents required for synthesis of double-stranded cDNA from mRNA and subsequent ligation into a suitable vector.
Build: Sat Nov 17 23:53:08 EST 2018 (commit: a759bb7). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 • 301-435-0888. ...
Bcl-2-associated transcription factor 1 is a Bcl-2 family protein in humans that is encoded by the BCLAF1 gene. This gene encodes a transcriptional repressor that interacts with several members of the BCL-2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different protein isoforms have been found for this gene. GRCh38: Ensembl release 89: ENSG00000029363 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000037608 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Nagase T, Seki N, Ishikawa K, Tanaka A, Nomura N (Nov 1996). Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell ...
Breakthrough of antimicrobial peptides (AMP) is to a large extent based on testing of fractions of organic samples in bacterial development inhibition assays. goals. had been at that correct period just obtainable seeing that clones from a cDNA collection. 5 The overall idea was to create and characterize this peptide without prior direct BINA chemical or isolation synthesis. AMP Production Because of the little BINA bit of Rabbit polyclonal to PLSCR1. genuine peptide directly retrieved from isolates additional studies always rely on a technique to recover even more of the materials of interest. That is essential to exploit their setting of actions and their pharmaceutical potential. Since it converted out in addition it is among the larger challenges when learning more technical AMPs. Generally you can find three different techniques that may be used: immediate isolation of peptides from organic sources chemical substance synthesis or recombinant manifestation of peptides in transgenic ...
Martin, D.; Bohlmann, J., 2004: Identification of Vitis vinifera (-)-a-terpineol synthase by in silico screening of full-length cDNA ESTs and functional characterization of recombinant terpene synthase
A variation on differential display, restriction‐mediated differential display (RMDD) presents an alternative approach to the fragment display technologies
Background A limiting factor of cDNA microarray technology is the need for a substantial amount of RNA per labeling reaction. Thus, 20-200 micro-grams total RNA or 0.5-2 micro-grams poly (A) RNA is typically ...
x-charset gb2312,Hi, All, Does anybody knows how to access Rikens(Japan) full length Arabidopsis cDNA? There is a page maintained at http://www.gsc.riken.go.jp/Plant/database/index.html. Awfully, it does not contain a serch engine or Tools like Blast or Fasta etc.And it obviously is not a complete package. I would like to download the cDNA sequences. Thanks for your kindly inputs. Baiqing LIN --- ,/x-charset ...
To whom it may concern, I am looking for a cDNA expression library of wheat or barley made of reproductive organs, preferably reproductive buds. Please contact. Thanks in advance, Avi. ----------------------------------------------------------------------- Avi Golan ...
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KIAA1543兔多克隆抗体(ab122864)可与人样本反应并经IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Development of a method for mRNA differential display in filamentous fungi: comparison of mRNA differential display reverse transcription polymerase chain reaction and cDNA amplified fragment length polymorphism in Leptosphaeria maculans
TY - JOUR. T1 - Novel patterns of gene expression in pituitary adenomas identified by complementary deoxyribonucleic acid microarrays and quantitative reverse transcription-polymerase chain reaction. AU - Evans, Chheng Orn. AU - Young, Andrew N.. AU - Brown, Milton R.. AU - Brat, Daniel J.. AU - Parks, John S.. AU - Neish, Andrew S.. AU - Oyesiku, Nelson M.. PY - 2001. Y1 - 2001. N2 - Pituitary adenomas account for approximately 10% of intracranial tumors, but little is known of the oncogenesis of these tumors. The identification of tumor-specific genes may further elucidate the pathways of tumor formation. We used complementary DNA microarrays to examine gene expression profiles in nonfunctioning, PRL, GH, and ACTH secreting adenomas, compared with normal pituitary. Microarray analysis showed that 128 of 7075 genes examined were differentially expressed. We then analyzed three genes with unique expression patterns and oncogenic importance by RT-real time quantitative PCR in 37 pituitaries. ...
Definition of differential display in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is differential display? Meaning of differential display as a finance term. What does differential display mean in finance?
TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal ),RT-PCR,PCR, RT-PCR, qPCR, qRT-PCR,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOv
Looking for differential display? Find out information about differential display. double density. data dictionary. Deployment Descriptor. A Unix copy command with special options suitable for block-oriented devices; it was often used in... Explanation of differential display
First-strand cDNA Synthesis System for Quantitative RT-PCR has been designed for the highest efficiency conversion of RNA to cDNA and is fully optimized for quantitative real-time PCR applications.
Mouse cDNA clones for a putative Ah receptor have been isolated from a cDNA library of mRNA from Hepa-1 cells by an oligonucleotide probe produced by PCR with a pair of primers which was synthesized according to the reported N-terminal sequence of 26 amino acids. The cDNA clones encode a polypeptide …
OriGene offers comprehensive product solutions for studying human protein kinases. Our functional kinome cDNA collection was featured in a Cell publication in 2008.
Purified Human Fetal Brain Cytoplasmic Lysate from Creative Biomart. Human Fetal Brain Cytoplasmic Lysate can be used for research.
QIAGENs single cell NGS solutions combine industry-leading performance, robustness, and sensitivity to deliver exceptional data quality. Our complete solutions cover the entire single cell NGS workflow, starting from single cells and ending with PCR-free NGS libraries, all with a single kit. Whether you are analyzing isolated single cells or complex tissues for sequence and structural variants, conducting PGD research, analyzing gene expression and detecting rare transcripts, or generating de novo genomes from a single bacterium, QIAseqs single cell NGS kits provide you with unprecedented sensitivity and accuracy at competitive all-in pricing that allows you to analyze more cells. Discover QIAseq solutions for single cell NGS today ...
METHOD XYZToLab(xyzval, wpoint) xyz=[xyzval[0]/wpoint[0],xyzval[1]/wpoint[1],xyzval[2]/wpoint[2]] i=0 while i , 3 if xyz[i] , 216.0 / 24389 // See BruceLindbloom.com xyz[i]=pow(xyz[i], 1.0/3.0) else kappa=24389.0/27 // See BruceLindbloom.com xyz[i]=(16.0 + kappa*xyz[i]) / 116 end i=i+1 end return [116.0*xyz[1] - 16, 500 * (xyz[0] - xyz[1]), 200 * (xyz[1] - xyz[2])] END METHOD METHOD LabToXYZ(lab,wpoint) fy=(lab[0]+16)/116.0 fx=fy+lab[1]/500.0 fz=fy-lab[2]/200.0 fxcb=fx*fx*fx fzcb=fz*fz*fz xyz=[fxcb, 0, fzcb] eps=216.0/24389 // See BruceLindbloom.com if fxcb ,= eps: xyz[0]=(108.0*fx/841)-432.0/24389 if fzcb ,= eps: xyz[2]=(108.0*fz/841)-432.0/24389 if lab[0] , 8 // See BruceLindbloom.com xyz[1]=pow(((lab[0]+16)/116.0), 3.0) else xyz[1]=lab[0]*27.0/24389 // See BruceLindbloom.com end xyz[0]=xyz[0]*wpoint[0] xyz[1]=xyz[1]*wpoint[1] xyz[2]=xyz[2]*wpoint[2] return xyz END METHOD METHOD SRGBToLab(rgb) return XYZToLab(XYZFromsRGB(rgb), [0.9504559270516716, 1, 1.0890577507598784]) END METHOD METHOD ...
TransOMICs cDNA clone collections are derived from the rigorously sequenced Mammalian Genome Collection created by the NIH and are 100% guaranteed to match the Genbank sequence.
This book offers practical advice to researchers interested in using the technique of RT-PCR differential display to help them understand the function of individual genes in specific tissues or cells of an organism, either as part of normal development and aging, or in disease processes.
With enhanced promotor, GSK3B cDNA ORF Clone, Mouse in pCMV3-N-OFPSpark is expression-ready, and confirmed by full-length sequence & expression validation
Cloning of Eg7 cDNA. (A) The open box represents the coding region (1,360 amino acids). Eg7.1-Eg7.4 correspond to partial cDNAs obtained by screening a Xe
View mouse BC052040 Chr2:115581716-115778768 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
Basics of microarrays Petter Mostad 2003 Why microarrays? Microarrays work by hybridizing strands of DNA in a sample against complementary DNA in spots on a chip. Expression analysis measure relative amounts
গল্পের শুরু ১৮১৫ সালে লস এঞ্জেলসে এক মুভি Stuntman এর ভয়াবহ পাগলাটে এক লাফ দিয়ে। রয় ওয়াকার নামে এই ভদ্রলোক Stuntman হিসেবে তার জীবনের প্রথম মুভিতে রেল ব্রিজ হতে এক অকল্পনীয় লাফ দিয়ে নিজেকে প্রায় পঙ্গু করে ফেলে হাসপাতালে ভর্তি হন। আর কমলালেবুর বাগানে কমলা তুলতে গিয়ে হাত ভেঙ্গে ফেলা আলেকজান্দ্রিয়া নামে এক ছোট্ট পরীও ভর্তি হয় ঐ হাসপাতালে। এক সকালে সে তার প্রিয় নার্সকে চরকা কাটা এক ...
Cats: they are the subject of millions of videos and hundreds of memes. This video has answers to some of the biggest cat-related questions out there: Why does catnip make most cats go crazy? How does kitty litter clump? And what does it mean ...