For those of you who like the cutting edge or want to help the KDE developers continue their phenomenal development pace, but arent yet ready to compile from CVS, the KDE Project has just released KDE 2.2alpha1. A list of some of the significant additions and improvements versus the recent 2.1 release is below (please add any unlisted significant changes in the comments section), as well as a list of currently available pre-compiled binaries (please read the KDE Binary Packages Policy). Please bear in mind that this is an alpha release and those enjoying a stable desktop should use KDE 2.1.1.
For those of you who like the cutting edge or want to help the KDE developers continue their phenomenal development pace, but arent yet ready to compile from CVS, the KDE Project has just released KDE 2.2alpha1. A list of some of the significant additions and improvements versus the recent 2.1 release is below (please add any unlisted significant changes in the comments section), as well as a list of currently available pre-compiled binaries (please read the KDE Binary Packages Policy). Please bear in mind that this is an alpha release and those enjoying a stable desktop should use KDE 2.1.1.
The objective of the present study was to investigate in rabbit corpora lutea (CL), at both the cellular and molecular level, intraluteal cyclooxygenase (COX)-1, COX-2 and prostaglandin (PG) E2-9-ketoreductase (PGE2-9-K) enzymatic activities as well as in vitro PGE2 and PGF2alpha synthesis following PGF2alpha treatment at either early- (day-4) or mid-luteal (day-9) stage of pseudopregnancy. By immunohistochemistry, positive staining for COX-2 was localized in luteal and endothelial cells of stromal arteries at both the stages. In CL of both stages, basal COX-2 mRNA levels were poorly expressed, but rose (P , 0.01) 4- to 10-fold 1.5-6 h after treatment and then gradually decreased within 24 h. Compared to mid-stage, day-4 CL had lower (P , 0.01) COX-2 and PGE2-9-K basal activities, and PGF2alpha synthesis rate, but higher (P , 0.01) PGE2 production. Independent of luteal stage, PGF2alpha treatment did not affect COX-1 activity. In day-4 CL, PGF2alpha induced an increase (P , 0.01) in both COX-2 ...
In this study we report that 8-iso-PGF2α, a major and stable product of lipid peroxidation, was potent in causing cell death of cultured brain microvascular endothelial cells as well as of ex vivo preparation of microvessels but not of human umbilical vein endothelium, smooth muscle, or astroglia; another related isoprostane, 8-iso-PGE2, did not trigger endothelial cell death. 8-Iso-PGF2α-induced cytotoxicity was TXA2 dependent and mostly of oncotic nature. Associated with these effects, intraventricular injection of 8-iso-PGF2α caused cystlike formations in the periventricular area.. The nature of cell death induced by 8-iso-PGF2α seems to be primarily oncotic rather than apoptotic necrosis (Figure 2). This inference is supported by the data that 8-iso-PGF2α caused ,8% of cell DNA fragmentation, a time-dependent increase in PI incorporation and LDH release, indicative of membrane disruption (Figure 2), and its effects, as well as those of its mediator TXA2, were unaltered by caspase ...
These results suggest that repeated sauna therapy may protect against oxidative stress, which leads to the prevention of atherosclerosis.
Endothelin-1 stimulates prostaglandin F2 alpha release from human endometrium.: Despite a key role in the pathogenesis of menorrhagia, the factors controlling t
Lutalyse 5 mg/ml Injection 30ml. Cattle To more effectively control the time of oestrus in cycling cows. To treat cows which have...
Our objective was to evaluate the effect of a second PGF2α treatment (25 mg of dinoprost) or a double dose of PGF2α (50 mg of dinoprost) during a Resynch protocol on luteal regression and pregnancies per artificial insemination (P/AI) in lactating dairy cows. Lactating Holstein cows (n = 1,100) were randomly assigned at a nonpregnancy diagnosis to receive (1) Ovsynch (control: 100 µg of GnRH; 7 d, 25 mg of PGF2α; 56 h, 100 µg of GnRH), (2) Ovsynch with a second PGF2α treatment (GPPG: 100 µg of GnRH; 7 d, 25 mg of PGF2α; 24 h, 25 mg of PGF2α; 32 h, 100 µg of GnRH), or (3) Ovsynch with a double dose of PGF2α (GDDP: 100 µg of GnRH; 7 d, 50 mg of PGF2α; 56 h, 100 µg of GnRH ...
Advice for mothers using Unoprostone (Rescula) while breastfeeding. Includes possible effects on breastfed infants and lactation.
Anonymous Access: cvs -d :pserver:[email protected]:/var/cvsroot co gentoo-x86/media-gfx/pixels2pgf Developer Access: cvs -d :ext:${USER}@cvs.gentoo.org:/var/cvsroot co gentoo-x86/media-gfx/pixels2pgf More Information: http://anoncvs.gentoo.org/ ...
Elevated level of free 8-iso-prostaglandin F2alpha in the decidua basalis of women with preeclampsia. Increased contents of phospholipids, cholesterol, and lipid peroxides in decidua basalis in women with preeclampsia
Mouse 8-iso-PGF2α(8-isoprostane) ELISA Kit from ELISA Genie is a pre-coated immunoassay with a sensitivity of 9.375pg/ml and a range of 15.625-1000pg/ml and has been designed to measure Mouse 8-iso-PGF2α(8-isoprostane) ELISA Kit in serum, plasma & cell culture supernatant samples. The Mouse 8-iso-PGF2α(8-isoprostane) ELISA Kit accurately measures natural Mouse 8-iso-PGF2α(8-isoprostane) levels quantified versus standard curves obtained and is based on antibodies raised against a recombinant Mouse 8-iso-PGF2α(8-isoprostane) peptide.
Isoprostanes are stable products of arachidonic acid peroxidation and are regarded as the most reliable markers of oxidative stress in vivo. Here we describe the LC-MS/MS procedure enabling simultaneous determination of four regioisomers (8-iso prostaglandin F2α, 8-iso-15(R)-prostaglandin F2α, 11β-prostaglandin F2α, 15(R)-prostaglandin F2α) in plasma samples collected from mice. The four plasma isoprostanes are determined by LC-ESI-MS/MS with deuterated 8-iso-PGF2α-d4 as an internal standard (I.S.). For plasma samples spiked with the isoprostanes at a level of 200 pg/mL each, the method imprecision has been below 7.1% and mean inaccuracy equaled 8.7%. The applicability of the proposed approach has been verified by the assessment of changes in isoprostane levels in plasma samples derived from mice exposed to tert-butyl hydroperoxide (TBHP), a model inducer of oxidative stress, or to antitumor drug doxorubicin (DOX) known for potent stimulation of redox cycling. Compared to the control group of
Oxidative stress and inflammation are supposed to be the key players of several acute and chronic diseases, and also for progressive aging process. Eicosanoids, especially prostaglandin F-2 alpha (PGF(2 alpha)) and F-2-isoprostanes are endogenous compounds that are involved both in physiology and the above mentioned pathologies. These compounds are biosynthesized mainly from esterified arachidonic acid through both enzymatic and non-enzymatic free radical-catalysed reactions in vivo, respectively. They have shown to possess potent biological activities in addition to their application as biomarkers of oxidative stress and inflammation. Recent advancement of methodologies has made it possible to quantify these compounds more reliably and apply them in various in vivo studies successfully. Today, experimental and clinical studies have revealed that both PGF(2 alpha) and F-2-isoprostanes are involved in severe acute or chronic inflammatory conditions such as rheumatic diseases, asthma, risk factors ...
Answer:Lutalyse is a brand name for a natural prostaglandin. There are several different prostaglandins on the market, some of which are synthetic. The synthetic ones are not used in cats since they are likely too powerful. So we only use Lutalyse, which is specifically prostaglandin F-2alpha. An alternate name for it you will sometimes see used is dinoprost tromethamine. Lutalyse is actually only labeled for use in cattle, not small animals, despite its long history of use in dogs and cats. You are going to have to indulge me in a short lesson on reproduction to explain why this drug is used differently in large animals versus cats. In cattle, it is a luteolytic agent. This means that it is able to shut down a structure on the ovary called the corpus luteum (CL). A CL forms on the ovary for every ovarian follicle that ovulates an egg. The function of the CL is to produce progesterone, which is the dominant hormone in pregnancy (and false pregnancy). So while the CLs are producing progesterone, ...
File scanned at 300 ppi (Monochrome, 256 Grayscale) using Capture Perfect 3.0.82 on a Canon DR-9080C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR ...
Hey everyone :) Im new to the site, so it will be cool to see the stuff you guys post in the future after Christmas time. I wanted to show you...
SUGINO Norihiro , OKUDA Kiyoshi Luteolysis is defined as the loss of function and subsequent involution of the luteal structure. The luteolytic process is usually subdivided, whereby the decline in progesterone is described as funct … Journal of Reproduction and Development 53(5), 977-986, 2007-10-01 J-STAGE Ichushi Web References (65) Cited by (4) ...
With heifers given two injections of Lutalyse 11 days apart, and inseminated 80 hours after the second injection, only 20% in heat before 48 hours conceived compared with 65% that were in heat 48 to 80 hours after injection ...
TY - JOUR. T1 - Influence of prostaglandin F2α analogues on the secretory function of bovine luteal cells and ovarian arterial contractility in vitro. AU - Korzekwa, A. J.. AU - Lukasik, K.. AU - Pilawski, W.. AU - Piotrowska-Tomala, K. K.. AU - Jaroszewski, J. J.. AU - Yoshioka, S.. AU - Okuda, K.. AU - Skarzynski, D. J.. PY - 2014/1. Y1 - 2014/1. N2 - Although prostaglandin (PG) F2α analogues are routinely used for oestrus synchronisation in cattle, their effects on the function of the bovine corpus luteum (CL), and on ovarian arterial contractility, may not reflect the physiological effects of endogenous PGF2α. In the first of two related experiments, the effects of different analogues of PGF2α (aPGF2α) on the secretory function and apoptosis of cultured bovine cells of the CL were assessed. Enzymatically-isolated bovine luteal cells (from between days 8 and 12 of the oestrous cycle), were stimulated for 24h with naturally-occurring PGF2α or aPGF2α (dinoprost, cloprostenol or ...
Oxidative stress is assumed to be the key underlying factor in the pathogenesis of many common diseases. This study describes the basal levels of 8-iso-PGF(2alpha ), a major F(2)-isoprostane and an in vivo oxidative stress biomarker in healthy subjects from three countries, namely Italy, Poland and Sweden, in relation to their smoking habits, age and gender. It studied urinary 8-iso-PGF(2alpha ) in 588 subjects from Sweden (n=220), Italy (n=203) and Poland (n=165). Polish subjects had the highest levels of F(2)-isoprostanes followed by the Swedish and Italians when adjusted for smoking, age, sex and creatinine and the inter-country differences were statistically significant. Smokers had significantly higher levels of 8-iso-PGF(2alpha ) compared to non-smokers in all countries and there was a moderate decrease with age. Women had only slightly lower 8-iso-PGF(2alpha ) than men. There is a difference in F(2)-isoprostane levels in vivo between countries. Smoking, age and gender affect isoprostane ...
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PubMed journal article Increases in urinary 9alpha,11beta-prostaglandin f2 indicate mast cell activation in wine-induced asthm were found in PRIME PubMed. Download Prime PubMed App to iPhone or iPad.
Ma, T.; Jiang, H.; Gao, Y.; Zhao, Y.; Dai, L.; Xiong, Q.; Xu, Y.; Zhao, Z.; Zhang, J., 2012: Microarray analysis of differentially expressed microRNAs in non-regressed and regressed bovine corpus luteum tissue; microRNA-378 may suppress luteal cell apoptosis by targeting the interferon gamma receptor 1 gene
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Progesterone administered before breeding may increase estrous expression and subsequent fertility in lactating dairy cows. Holstein cows (n=302) were assigned randomly at calving to three groups. Cows received no treatment (controls), one injection of prostaglandin F 2-alpha (PGF), or progesterone plus one injection of PGF (PRID + PGF). The average interval to estrus was 5 days shorter in PRID + PGF cows compared to cows given only PGF. In addition, more cows were observed in estrus 2 to 5 days after treatment in the PRID + PGF group compared to the PGF group. Average days from calving to conception were reduced in PRID + PGF cows by 15 to 20 days compared to control and PGF cows. We concluded that prebeeding progesterone in combination with PGF appears to enhance fertility ...
A study undertaken to characterise the oestrous cycle in Zebu cows following oestrous induction with PGF (Prostaglandin F2alpha) and examines the function of the subsequently formed CL (corpus luteum) by monitoring levels of peripheral plasma progesterone ...
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The forum for the discussion of the use of hormones in the mare to induce estrus; maintain pregnancy; abort foals; and also use of hormones to stimulate or quieten stallions.
A naturally occurring prostaglandin that has oxytocic, luteolytic, and abortifacient activities. Due to its vasocontractile properties, the compound has a variety of other biological actions ...
8 PROSTAGLANDIN F2a ANALOGUES 10. Ideal implantation is into the connective tissue layer between the mucosa and the rhabdospincter (24).
Thanks to the infrastructure that was developed during the Fedora 7 release cycle, Alpha is accompanied by live CDs of both the GNOME and KDE desktops, making testing possible without installing any software on an existing system. As the Alpha release is largely targeted at developers and contains many bleeding edge packages, this is the best method for less experienced users who want to get involved with testing. Alpha images can be downloaded from any of our mirrors by visiting this page . Remember that live images can be used on USB media via the livecd-iso-to-disk utility available in the livecd-tools package on existing Fedora systems; Refer to this article for more instructions. ...
Thanks to the infrastructure that was developed during the Fedora 7 release cycle, Alpha is accompanied by live CDs of both the GNOME and KDE desktops, making testing possible without installing any software on an existing system. As the Alpha release is largely targeted at developers and contains many bleeding edge packages, this is the best method for less experienced users who want to get involved with testing. Alpha images can be downloaded from any of our mirrors by visiting this page . Remember that live images can be used on USB media via the livecd-iso-to-disk utility available in the livecd-tools package on existing Fedora systems; Refer to this article for more instructions. ...
Palm has moved Ares, its Web-based development environment for WebOS applications, to an alpha release stage, a Palm official said in a blog earlier this month
The function calculates nonparametric indices for testing the validity of an ISOP-Model, suggested by Scheiblechner, for a given set of items. ...
Hi, Does anyone know how to show, by means of probability generating functions, that for large n and small p, (n →∞, p→0), such that
INTRODUCTION: The most recently discovered severe acute respiratory syndrome Coronavirus 2 (SARS-COV-2) that causes COVID-19, subjected the entire world in turmoil health-wise and economically. With higher burden of malaria in Nigeria and other Sub-Saharan African countries coupled with fragile healthcare system and delivery, these may pose a threat in the diagnosis and management of COVID-19 patients co-infected with malaria. Free radicals have been implicated in the progression and pathogenesis of malaria and COVID-19 through Fentons reaction and cytokine storm respectively. METHODS: the current research comprises of seventy-four (74) participants; 20 apparently healthy controls and 54 COVID-19 patients (20 among which were co-infected with malaria). Serum levels of 8-iso PGF2α and Alphatocopherol were determined among the study participants using ELISA technique and colorimetric assay, respectively. RESULTS: results revealed statistically significant elevation of 8-iso PGF2α in COVID-19 patients
The present study reveals that 15-F2t-IsoP causes greater constriction of periventricular microvessels of the fetus than of newborn and juvenile animals due to greater thromboxane generation through a previously undescribed mechanism of action of 15-F2t-IsoP, which seems to involve activation of distinct calcium channels and an interaction between vascular and perivascular cells. Data indicate that 15-F2t-IsoP elicits vasoconstriction by increasing the release of thromboxane from brain astroglial and endothelial but not vascular smooth muscle cells; the increased thromboxane synthesis seems to result from increased entry of extracellular calcium into cells, possibly through N-type voltage-dependent calcium channels in astrocytes and non-voltage-dependent calcium channels in endothelial cells.. The effects of 15-F2t-IsoP have been found to be markedly inhibited by thromboxane receptor blockers7 22 23 24 ; however, ligand binding studies suggest that 15-F2t-IsoP does not directly interact with the ...
Prostaglandin F2α (PGF2α in prostanoid nomenclature), pharmaceutically termed dinoprost (INN), is a naturally occurring prostaglandin used in medicine to induce labor and as an abortifacient. In domestic mammals, it is produced by the uterus when stimulated by oxytocin, in the event that there has been no implantation during the luteal phase. It acts on the corpus luteum to cause luteolysis, forming a corpus albicans and stopping the production of progesterone. Action of PGF2α is dependent on the number of receptors on the corpus luteum membrane. The PGF2α isoform 8-iso-PGF2α was found in significantly increased amounts in patients with endometriosis, thus being a potential causative link in endometriosis-associated oxidative stress. PGF2α acts by binding to the prostaglandin F2α receptor. It is released in response to an increase in oxytocin levels in the uterus, and stimulates both luteolytic activity and the release of oxytocin. Because PGF2α is linked with an increase in uterine ...
This study investigated whether or not there was an overall difference in the pulmonary oxidative marker, 8-isoprostane (PGF2-a), found in exhaled breath condensate, during pregnancy versus the non-pregnant state. The utility of this information was important secondary to the effect of maternal asthma on pregnancy and outcome, as it has been demonstrated in past studies that overall pregnant females with asthma have been shown to have an increased risk of exacerbation requiring medical intervention.The primary goal of this study sought to determine if there is a difference in PGF2a levels in the exhaled breath condensate of pregnant versus non-pregnant females. In order to achieve this goal a cross-sectional study was performed consisting of two groups and were compared to one another. A group of 16 healthy, non-pregnant females aged 18-35 years old was compared to a group of 6 healthy, pregnant females in their third trimester of pregnancy. Both groups exhaled breath condensate was collected and 8
Recent studies suggest that chemokines may mediate the luteolytic action of PGF2α (PGF). Our objective was to identify chemokines induced by PGF in vivo and to determine the effects of IL8 on specific luteal cell types in vitro. Midcycle cows were injected with saline or PGF, ovaries were removed after 0.5 - 4 h and chemokine expression was analyzed by qPCR. In vitro expression of IL8 was analyzed after PGF administration and with cell signaling inhibitors to determine the mechanism of PGF-induced chemokine expression. Purified neutrophils were analyzed for migration and activation in response to IL8 and PGF. Purified luteal cell types (steroidogenic, endothelial and fibroblast cells) were used to identify which cells respond to chemokines. Neutrophils and peripheral blood mononuclear cells (PBMCs) were co-cultured with steroidogenic cells to determine their effect on progesterone production. IL8, CXCL2, CCL2, and CCL8 transcripts were rapidly increased following PGF treatment in vivo and. The
Background: Cigarette smoking is a risk factor for several diseases including cardiovascular disease, chronic obstructive pulmonary disease and lung cancer, but the role of specific smoke constituents in these diseases has not been clearly established. Methods: The relationship between biomarkers of potential harm (BOPH), associated with inflammation [white blood cell (WBC), high sensitivity C-reactive protein (hs-CRP), fibrinogen, von Willebrand factor (vWF)], oxidative stress [8-epi-prostaglandin F2α (8-epiPGF2α)] and platelet activation -[11-dehydro-thromboxin B2 (11-dehTxB2)], and machine measured tar yields (grouped into 4 categories), biomarkers of exposure (BOE) to cigarette smoke, nicotine and five metabolites [(Nicotine equivalents) - NE)], 4-methylnitrosamino-1-(3-pyridyl)-1-butanol (total NNAL), carboxyhemoglobin (COHb), 1-hydroxypyrene (1-OHP), 3-hydroxypropylmercapturic acid (3-HPMA) and monohydroxybutenyl-mercapturic acid (MHBMA), was investigated in 3,585 adult smokers (AS) and ...
The origin and physiological significance of high pulses of prostaglandin F2α (PGF2α) in uterine venous blood that occur 2-3 days after luteolysis are not well understood. We studied the relationship between contractions of the uterus evoked by exogenous oxytocin (OT) and PGF2α concentration in uterine venous blood on day 17 of the porcine oestrous cycle. The infusion of OT into the uterine artery produced an immediate increase in the uterine intraluminal pressure (UIP) (p , 0.001) and a simultaneous elevation in PGF2α concentration in uterine venous blood (p , 0.0001). The infusion of indomethacin (IND) into the uterine artery slightly decreased PGF2α concentration in uterine venous blood, but it did not suppress uterine contraction or the rapid increase in PGF2α concentration in uterine venous blood just after OT infusion (p , 0.0001), which was lower that in gilts not treated with IND. We conclude that the spikes of PGF2α concentration in uterine venous blood occurring after OT ...
Prostaglandin D2 (PGD2) was recently found to be stereospecifically converted to the compound (5Z,13E)-(15S)-9 alpha,11 beta,15-trihydroxyprosta-5,13-dien-1-oic acid (9 alpha,11 beta-PGF2) by a human liver cytosolic NADPH-dependent 11-ketoreductase enzyme. Because PGD2 is a potent bronchoconstrictor …
In ruminants, uterine pulses of prostaglandin (PG) F 2α characterize luteolysis, while increased PGE 2 /PGE 1 distinguish early pregnancy. This study evaluated intrauterine (IU) infusions of PGF 2α and PGE 1 pulses on corpus luteum (CL) function and gene expression. Cows on day 10 of estrous cycle received 4 IU infusions (every 6 h; n = 5/treatment) of saline, PGE 1 (2 mg PGE 1), PGF 2α (0.25 mg PGF 2α), or PGE 1 + PGF 2α. A luteal biopsy was collected at 30 min after third infusion for determination of gene expression by RNA-Seq. As expected, IU pulses of PGF 2α decreased (P , 0.01) P4 luteal volume. However, there were no differences in circulating P4 or luteal volume between saline, PGE 1, and PGE 1 + PGF 2α, indicating inhibition of PGF 2α -induced luteolysis by IU pulses of PGE 1. After third pulse of PGF 2α, luteal expression of 955 genes were altered (false discovery rate [FDR] , 0.01), representing both typical and novel luteolytic transcriptomic changes. Surprisingly, after ...
In the current study, our results demonstrated that the IκB/NF-κB signaling pathway was involved in the expression and activation of MMP-2 in human ciliary muscle cells. Downregulation of IκBα by siRNA resulted in significant translocation of NF-κBp65 from the cytoplasm to the nucleus after 24 h to 72 h. The secretion of MMP-2 increased as well as the activation from pro-MMP-2 to active-MMP-2. In addition, the expression of MT1-MMP increased while the expression of TIMP-2 reduced both in mRNA and protein levels. It is implied from these results that NF-κB is an important transcriptional factor involved in the control of uveoscleral outflow through the promotion of MMP-2 expression. It has been reported that NF-κB is involved in various activities and plays a key role in multiple biological processes including chemical stress, physical stress, physiologic stress, receptor ligands, proinflammatory cytokines, and apoptosis. At the same time, there are also multiple and complex signal ...
Endometrial cells from the uteri of pregnant and nonpregnant cattle were cultured. The presence of one or both of two viruses, noncytopathic mucosal disease virus and bovine syncytial virus, was demonstrated in seven of 19 endometria investigated. It was necessary to subculture the cells an average of four times to detect the viral infections. Difficulties were encountered in producing endometrial cell cultures from cows at term or near the end of term and also from older animals. The infections detected may be significant because both of the viruses isolated are capable of infecting the bovine fetus in utero and mucosal disease virus has been associated with bovine fetal diseases.
Definition of f2-isoprostanes in the Definitions.net dictionary. Meaning of f2-isoprostanes. What does f2-isoprostanes mean? Information and translations of f2-isoprostanes in the most comprehensive dictionary definitions resource on the web.
We investigated whether an EPA-enriched high-fat meal could improve postprandial vascular function. Seventeen healthy men ingested 2 test meals (51 g fat), 1 wk apart, in random order: 5 g EPA plus high-oleic sunflower oil (HOS) vs. HOS only. A second high-fat meal (44 g fat), the same on both study days, was provided 4 h later. Blood pressure and arterial function were measured using digital volume pulse (DVP) to derive a stiffness index (DVP-SI) and reflection index in fasting subjects at 3 and 6 h following the test meal. Blood samples were taken following the test meal for plasma 8-isoprostane F2alpha, nitric oxide (NO) metabolites (NOx), glucose, insulin, triacylglycerol, and fatty acid analysis ...
Sboros V, Averkiou M, Lampaskis M, et al. Imaging of the Ovine Corpus Luteum Microcirculation with Contrast Ultrasound. Ultrasound in Medicine & Biology. 2011;37:59-68. doi:10.1016/j.ultrasmedbio.2010.10.013 ...