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This paper presents a new hermetic encapsulation method for negative-pressure-driven polydimethylsiloxane (PDMS) microfluidic devices. The hermetic materials used in this encapsulation are mainly para
An electronic microvalve design and fabrication process for a miniature gas valve. In this microvalve design an objective is to minimize the operating voltage of the valve and minimize the force necessary to close the valve and hold it tightly closed against high gas pressures. The microvalve is an integral structure made on one piece of silicon and is a flow through valve with inlet and outlet on opposite sides of the silicon wafer.
TY - JOUR. T1 - PDMS microchannel surface modification with teflon for algal lipid research. AU - Park, Jae Woo. AU - Na, Sangcheol. AU - Kang, Myeongwoo. AU - Sim, Sang Jun. AU - Jeon, Noo Li. PY - 2017/9/1. Y1 - 2017/9/1. N2 - This paper presents a simple method for modifying the polydimethylsiloxane (PDMS) microfluidic channels with Teflon for algal lipid research. When culturing and staining algae inside microfluidic devices, the small molecule dyes absorbed by the microchannel surface render it difficult for imaging and quantification. PDMS surface coated with Teflon-AF resists the absorption of hydrophobic dye molecules (i.e., BODIPY and Nile red) as confirmed using fluorescence microscopy. Here, we introduce a surface modification of PDMS microchannel using Teflon-AF using a procedure of filling and drying to directly treat the PDMS surface with perfluorinated materials. This method can be used to prevent the absorption of fluorescent probe and obtain clear fluorescence micrographs ...
Microphase separation behavior on the surfaces of poly(dimethylsiloxane)-block-poly(2,2,3,3,4,4,4-heptafluorobutyl methacrylate) (PDMS-b-PHFBMA) diblock copolymer coatings was investigated. The PDMS-b-PHFBMA diblock copolymers were successfully synthesized via atom transfer radical polymerization (ATRP). The chemical structure of the copolymers was characterized by nuclear magnetic resonance and Fourier transform infrared spectroscopy. Surface composition was studied by X-ray photoelectron spectroscopy. Copolymer microstructure was investigated by atomic force microscopy. The microstructure observations show that well-organized phase-separated surfaces consist of hydrophobic domain from PDMS segments and more hydrophobic domain from PHFBMA segments in the copolymers. The increase in the PHFBMA content can strengthen the microphase separation behavior in the PDMS-b-PHFBMA diblock copolymers. And the increase in the annealing temperature can also strengthen the microphase separation behavior in ...
Sigma-Aldrich offers Aldrich-433012, Poly(dimethylsiloxane), vinyl terminated for your research needs. Find product specific information including CAS, MSDS, protocols and references.
This paper presents a chip-scale random lasing action utilizing polydimethylsiloxane (PDMS) wrinkles with random periods as disordered medium. Nanoscale wrinkles with long range disorder structures are formed on the oxidized surface of a PDMS slab and confirmed by atomic force microscopy. Light multiply scattered at each PDMS wrinkle-dye interfaces is optically amplified in the presence of pump gain. The shift of laser emission wavelength when pumping at different regions indicates the randomness of the winkle period. In addition, a relatively low threshold of about 27 μJ/mm{sup 2} is realized, which is comparable with traditional optofluidic dye laser. This is due to the unique sinusoidal Bragg-grating-like random structure. Contrast to conventional microfluidic dye laser that inevitably requires the accurate design and implementation of microcavity to provide optical feedback, the convenience in both fabrication and operation makes PDMS wrinkle based random laser a promising underlying ...
Pyrene-substituted polyhydromethylsiloxanes (PHMS-Py-x) were synthesised by the hydrosilylation reaction of prop-3-enyloxymethylpyrene with polyhydromethylsiloxane (M-n = 3700). The ratio of pyrene substituent to Si-H unit was varied to afford a range of pyrene-functionalised polysiloxanes. These copolymers were subsequently incorporated into polydimethylsiloxane (PDMS) elastomers by curing via either Pt(0) catalysed hydrosilylation with divinyl-terminated PDMS (M-n = 186) and tetrakis(dimethylsiloxy) silane, or Sn(II) catalysed condensation with alpha,omega-dihydroxyPDMS (M-n = 26 000) and tetraethoxysilane. An alternative method involving the synthesis and integration of [3-(pyren-1-ylmethoxy)propyl]triethoxysilane (Py-TEOS) into PDMS elastomers was also investigated: a mixture of alpha,omega-dihydroxyPDMS (M-n = 26 000), tetraethoxysilane, and Py-TEOS was cured using an Sn( II) catalyst. Certain of the resulting fluorescent pyrene-labelled elastomers were studied by differential scanning ...
We report a porous and green three dimensional (3-D) polydimethylsiloxane (PDMS)-graphene sponge with hydrophobic and oleophilic properties using the sugar templating method. The prepared sponge exhibited high adsorption performance for the removal of petroleum products, organic solvents and emulsified oil-w
PolyDimethylsiloxane PDMS Affects Gene Expression in PC12 Cells Differentiating into Neuronal-Like Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
In the emerging field of MEMS microfluidics, micropumps and microvalves are two of the most important devices with a wide spectrum of applications such as programmable drug delivery systems, lab-on-a chip devices, µTAS (micro total analysis system), micro electric cooling applications etc. These microfluidic components are dominating the MEMS applications by virtue of their improved performance and reliability, cost and surface area reduction, etc. Micropumps convert the applied electrical energy into kinetic energy of the fluid, while microvalves maintain the directionality of fluid flow in a microfluidic channel. The aim of this work is to develop micropumps, microchannels and microvalves and combine them to develop a complete system that can be used in any of the said application fields with appropriate modifications. The micropump is modeled and simulated using COMSOL Multiphysics using solid stress-strain, piezoelectric and incompressible Navier-Stokes modes. In order to simulate the ...
Dive into the research topics of A polydimethylsiloxane diaphragm integrated with a sputtered thin film NdFeB magnet. Together they form a unique fingerprint. ...
Microfluidic lab-on-a-chip cell tradition methods have been gaining reputation by providing the potential for lowering the quantity of samples and reagents and higher management over mobile microenvironment. Polydimethylsiloxane (PDMS) is the generally used polymer for microfluidic cell tradition units due to a budget and simple fabrication methods, non-toxicity, biocompatibility, excessive gasoline permeability, and optical transparency. However, the intrinsic hydrophobic nature of PDMS makes cell seeding difficult when utilized on PDMS floor. The hydrophobicity of the PDMS floor additionally permits the non-specific absorption/adsorption of small molecules and biomolecules which may have an effect on the mobile behaviour and capabilities. Hydrophilic modification of PDMS floor is indispensable for profitable cell seeding. This evaluate collates totally different methods with their benefits and downsides which were used to enhance PDMS hydrophilicity to facilitate endothelial cells seeding in ...
Micropatterning is the art of miniaturisation of patterns. Especially used for electronics, it has recently[when?] become a standard in biomaterials engineering and for fundamental research on cellular biology by mean of soft lithography. It generally uses photolithography methods but many techniques have been developed [1]. In cellular biology, micropatterns can be used to control the geometry of adhesion and substrate rigidity. This tool helped scientists to discover how the environment influences processes such as the orientation of the cell division axis, organelle positioning, cytoskeleton rearrangement cell differentiation and directionality of cell migration.[2] [3] Micropatterns can be made on a wide range of substrates, from glass to polyacrylamide and Polydimethylsiloxane (PDMS). The polyacrylamide and PDMS in particular come into handy because they let scientists specifically regulate the stiffness of the substrate, and they allow researchers to measure cellular forces (traction force ...
Learn about the specialized features in COMSOL Multiphysics® for performing shape and topology optimization with the example of a Tesla microvalve.
The capillary burst microvalve offers an attractive means to regulate microliquid flow owing to its simple structure and operation process. However, there existed no rigorous theoretical work to elucidate how the valve works and consequently to predi
A self-priming and bubble tolerant planar micro-pump, which is fabricated by traditional technology, has been demonstrated and characterized. The micro-pump has a simple three-layered structure. Its two pump housings are made of polycarbonate and they are fabricated by computer numerical control (CNC) machine. The actuation membrane, which acts as the inlet and outlet valve membrane is cast in polydimethylsiloxane (PDMS). Using the PDMS membrane to act as the actuation membrane and valve membrane, we have solved the problem of sealing and poor compression ratio that most silicon based micro-pump faced. From the model of the membrane stroke volume, the flow rate of the pump is insensitive to the pump output pressure, and the output flow rate is linearly varying with actuating frequency. Flow rate up to 1000 ul/min of liquid has been achieved. More than 2m pump-head has been obtained when using water as the pumping medium. The robustness of the pump makes it suitable for disposable applications ...
Optical micropillar Bragg cavities of different diameters and coupled by a small bridge have been realized experimentally by means of a focused ion beam system. The resonator modes in these coupled microcavities are either localized in one pillar or delocalized over the whole photonic structure, a fact that could be exploited to control the coupling between two spatially separated quantum dots, i.e. placed in different pillars, via the enhanced electromagnetic field in such a coupled microcavity. A simplified two dimensional simulation has been used to predict the resonant wavelengths and design the optical modes in these coupled Bragg cavities.. ©2007 Optical Society of America. Full Article , PDF Article ...
A flow controller module comprising at least one micro flow sensor and a microvalve, integrated in a micro flow channel, is disclosed. The micro flow sensor comprises a pressure sensitive flow sensor. At the position of the micro flow sensor, the micro flow channel is provided with an orifice so that pressure of a flow may be enlarged to facilitate measurement of the flow speed. The microvalve comprises a silicon microbridge with a mesa structure and is driven by a voltage. The microvalve may operate under a normally closed mode or a normally open mode. Disclosed in this invention is also a flow sensor suited in the integrated flow controller module. Methods for preparing the flow sensor and the flow controller module are also disclosed.
The capillary driven flow in microchannel is a self-driven flow by the natural phenomenon called surface tension of the fluid. The gradients in surface tension force which influence the flow field in microchannel is generated by the modulation of contact angle through a defined hydrophilization of the PDMS (Polydimethylsiloxane) microchannel surface. PDMS which is hydrophobic in nature is treated with various surface treatments in order to convert it to hydrophilic. The contact angle made by the fluid with the PDMS microchannel surface is altered when the surface is converted from hydrophobic to hydrophilic. The flow rate of fluid in the microchannel is directly proportional to the hydrophilicity of that microchannel since the capillary force which is the driving force of the flow is dependent on the contact angle. Flow control mechanism of capillary driven flow in microchannel using non-mechanical forces is developed by treating the microchannel surfaces with various surface treatments. The ...
The artificial leaves designed by the authors were microfluidic devices fabricated from poly(dimethylsiloxane) (PDMS) and fixed to glass substrates (see Fig. 1). The devices possessed n parallel channels (of length = L = 35 mm, width = ω, height = h) separated by a distance d (that varied between 30µm and 1500µm); the channels were fixed between the glass substrate and a layer of PDMS with thickness δ (that varied between 45µm and 750µm). The width of the devices was W = 30 mm. Air with a controlled relative percent humidity (RH, ranged from 20% to 100%) was flowed over the artificial leaves at 1 L*min^-1. Plexiglass was kept 1 mm above the devices to ensure that water vapor diffused from the channels into an atmosphere of constant RH. The microfluidic devices were then connected to a beaker of water 30 cm below that rested on a computer-monitored mass balance. As water evaporated from the channels, water from the beaker would flow upward and into the device. Measurements of the ...
Purpose: : To develop surgical techniques and devices for cell sheet transplantation into the subretinal space. Methods: : Retinal pigment epithelial (RPE) cells were derived from induced pluripotent stem cells of cynomolgus monkeys and RPE cell sheets were prepared by the expansion of picked-up iPS cell-derived RPE cell colonies. RPE cell sheets were cut in the sizes which were fitted for transplantation into the subretinal space with the laser microdissection (LMD) system (PALM Microbeam; Carl Zeiss). The pneumatic balloon actuator (PBA) was made of polydimethylsiloxane (PDMS) membrane and driven by pneumatic pressure. Employing the principle of PBA, a square shaped device was retracted into the cylindrical shape whose diameter of 2mm. By using the device, we tried to perform sequential motions so as to introduce and transplant RPE sheets into the subretinal space. Results: : By using LMD system, RPE cell sheets were able to cut in any size and shape. RPE cell sheets were easily placed on the ...
SPECIFIC POLYMERS offers Research Program services to its customers to accompany them in there R&D activity by bringing an expertise in the synthesis of functional building-blocks, monomers and polymers and their formulation to develop high-performance resins and materials. Our objective is to support your research and find answers to your objectives by synthesizing and delivering the necessary amount of matters to your laboratories and thus validate proof of concepts in your field of research ...
SPECIFIC POLYMERS offers Research Program services to its customers to accompany them in there R&D activity by bringing an expertise in the synthesis of functional building-blocks, monomers and polymers and their formulation to develop high-performance resins and materials. Our objective is to support your research and find answers to your objectives by synthesizing and delivering the necessary amount of matters to your laboratories and thus validate proof of concepts in your field of research ...
To study the effect of the additional solvent treatment of the silane-coated master mold on PDMS molding, right before (undiluted) PDMS casting, some master molds were dipped into toluene or hexane for 1 min and dried with nitrogen gun. Results Effect of solvent treatment on PDMS filling into nanoholes Figure 1 shows the scanning electron microscopy (SEM) image of the master mold consisting of array of holes. with various diameters. There are a total selleck chemical of ten different diameters in the mold; shown here are representative three with diameters of 500, 300, and 120 nm (smallest). Figure 1d is the cross-sectional view of the holes with diameter of 300 nm near a large etched area in order to reveal the etched profile, which shows a nearly vertical profile with depth close to 1,000 nm. However, the hole could be slightly shallower for smaller diameters due to the difficulty for etching species to diffuse into and for etching products to get out of the holes. Smaller holes are not ...
Author(s): Calabrese DR, Wenning B, Finlay JA, Callow ME, Callow JA, Fischer D, Ober CK. Publication type: Article. Publication status: Published. Journal: Polymers for Advanced Technologies Year: 2015. Volume: 26. Issue: 7. Pages: 829-836. Print publication date: 01/07/2015. Online publication date: 30/04/2015. Acceptance date: 05/03/2015. ISSN (print): 1042-7147. ISSN (electronic): 1099-1581. Publisher: John Wiley & Sons, Inc.. URL: http://dx.doi.org/10.1002/pat.3515. DOI: 10.1002/pat.3515. ...
Tampere University of Technology is at the leading edge of technology development and a sought-after collaboration partner among the scientific and business communities. The University produces competent graduates who enter careers in the different sectors of society.. ...
When 3T3 fibroblasts are seeded onto the FN-coated PDMS substrates, their initial projected area, as observed through RICM, typically expands in a uniform fashion and in accordance with the underlying pattern: i.e. it expands radially in a circular form on the control substrate (figure 1b); and lengthwise in a rectangular form on the striped substrates (insets in figure 3a-c). It is worth noting that 3T3s on the wider stripes would also spread radially initially, and only expand lengthwise upon reaching the stripe edges. In addition, depending on initial position of the cell relative to an edge, the shape of the initial expanding area may range from fractions of a circle to a full circle (insets in figure 3a,b). Evident from the later RICM images in the insets, as time progresses, the uniformity and symmetry of the expanding cells projected area eventually breaks for all the substrate conditions, concurrent with the onset of perimeter undulations of the projected area. The spread curves follow ...
19. Jeong Hoon Lee, Yong-Ak Song, Jongyoon Han, Multiplexed Proteomic Sample Preconcentration Device Using Surface-Patterned Ion-Selective Membrane, Lab Chip,2008, 8, 596 - 601. (IF~5.82). 18. Jeong Hoon Lee, Yong-Ak Song, Steven R. Tannenbaum, Jongyoon Han, Increase of Reaction Rate and Sensitivity of Low-Abundance Enzyme Assay using Micro/Nanofluidic Preconcentration Chip, Anal. Chem., 80 (9), 3198 -3204, 2008 (IF~5.64). 17. Seok Chung, Jeong Hoon Lee, Moung-Woon Moon, Jongyoon Han, Roger D. Kamm, Non-Lithographic Wrinkle Nanochannels for Protein Preconcentration, Adv. Mater. 20 (16), 3011 -3016, 2008 (IF~19.7). 16. Jeong Hoon Lee, Seok Chung, Sung Jae Kim, Jongyoon Han, Poly((dimethylsiloxane)-Based Protein Preconcentration using a Nanogap Generated by Junction Gap Breakdown, Anal. Chem. 2007, 79(17); 6868-6873. (IF~5.64). 15. Sung Jae Kim, Ying-Chih Wang, Jeong Hoon Lee, Hongchul Jang, Jongyoon Han, Concentration polarization and nonlinear electrokinetic flow near a nanofluidic ...
Then the flux from the donor to the acceptor compartment is measured within 24h. The system does not need to go into steady state, it is sufficient to achieve a measurable change of concentration in the acceptor PDMS disk (see concentration profiles in Figure 1b). For very hydrophobic compounds, it would take too long to wait until the PDMS membrane is saturated. Therefore the membrane was preloaded prior to the experiment with half of the donor PDMS disk concentration (steady state concentration). It was experimentally confirmed that this membrane concentration did not change over the course of the experiment.. Using measured permeability values in PDMS-PAMPA system and measured partition coefficients between PDMS and water3, the artificial in-vitro membrane permeability was normalized with in-vivo elimination rate in small fish3. Figure 3 demonstrates that the measured permeability is proportional to the passive elimination rate constant in fish and can be used to predict the minimum in-vivo ...
INTRODUCTION: Microfluidics systems usually consist of materials like PMMA--poly(methyl methacrylate) and PDMS--poly(dimethylsiloxane) and not polystyrene (PS), which is usually used for cell culture. Cellular and molecular responses in cells grown on PS are well characterized due to decades of accumulated research. In contrast, the experience base is limited for materials used in microfludics chip fabrication. METHODS: The effect of different materials (PS, PMMA and perforated PMMA with a piece of PDMS underneath) on the growth and differentiation of PC12 (adrenal phaeochromocytoma) cells into neuronal-like cells was investigated using cell viability, cell cycle distribution, morphology, and gene expression analysis ...
Researchers demonstrate control of self-collapse behavior of polydimethylsiloxane (PDMS) stamps precisely at the nanoscale when integrated with chemical lift-off lithography to establish a new nanolithography method: self-collapse lithography.
Soo Sang Chae , Haesik Min , Jeong Hun Lee , Byounghar Hwang , Woong Mo Sung ,Woo Soon Jang , Young Bum Yoo , Jinyoung Oh , Jee Ho Park , Daeseung Kang ,Doseok Kim , Youn Sang Kim , and Hong Koo Baik, Fabrication of a Multidomain and Ultrafast-Switching Liquid Crystal Alignment Layer Using Contact Printing with a Poly(dimethylsiloxane) Stamp, Adv. Mater. (2013). html ...
Soo Sang Chae , Haesik Min , Jeong Hun Lee , Byounghar Hwang , Woong Mo Sung ,Woo Soon Jang , Young Bum Yoo , Jinyoung Oh , Jee Ho Park , Daeseung Kang ,Doseok Kim , Youn Sang Kim , and Hong Koo Baik, Fabrication of a Multidomain and Ultrafast-Switching Liquid Crystal Alignment Layer Using Contact Printing with a Poly(dimethylsiloxane) Stamp, Adv. Mater. (2013). html ...
a) The average number of cells dispensed into each micropillar in the actual chip when 80 cells were dispensed into 40 nL alginate spots in six Cellvitro ™ 532 chips, 78 ± 9 cells / chip (CV 12%) (b) ASFA ™ Scanner After scanning the Cellvitro ™ 532 chip using the Ez Analyzer, measure the number of cells on one micropillar using the Ez Analyzer (green: real live cells, red: analysis results). Number measurement result (3192 micropillars in 6 chips ...
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External forces are increasingly recognized as major regulators of cellular structure and function, yet the underlying mechanism by which cells sense forces and transduce them into intracellular biochemical signals and behavioral responses (mechanotransduction) is largely undetermined. To aid in the mechan
Single-molecule experiments are a long way from real-world applications, though. So the researchers created a 6 mm wide polymer disk by blending their mechanoacid with poly(dimethylsiloxane) and the dye rhodamine B, which turns pink when protonated. When they hit the disk with a hammer, the polymer released acid that slowly spread through the material and reacted with the dye to create a pink bruise. The researchers could estimate when the blow was struck by measuring the extent of the color change, and they were accurate to within a few minutes for periods up to half an hour. It marks the first example of a mechanoacid being used in a bulk material, the team says.. ...
Zhu, X.L., Zhang, Y., Chandra, D., Cheng, S.C., Kikkawa, J.M. and Yang, S. Two-dimensional photonic crystals with anisotropic unit cells imprinted from poly(dimethylsiloxane) membranes under elastic deformation. Appl. Phys. Lett. 93, doi:10.1063/1.3006061 (2008). * (Selected to November 10, 2008 issue of Virtual J. Nanoscale Sci. & Tech., Am. Inst. Phys. & Am. Phys. Soc ...
Sun N, Lee YT, Zhang RY, Kao R, Teng PC, Yang Y, Yang P, Wang JJ, Smalley M, Chen PJ, Kim M, Chou SJ, Bao L, Wang J, Zhang X, Qi D, Palomique J, Nissen N, Han SB, Sadeghi S, Finn RS, Saab S, Busuttil RW, Markovic D, Elashoff D, Yu HH, Li H, Heaney AP, Posadas E, You S, Yang JD, Pei R, Agopian VG, Tseng HR, Zhu Y. Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring. Nat Commun. 2020 09 07; 11(1):4489 ...
MOUNTAIN VIEW, California, and OSAKA, Japan, March 18-Picoliter Inc. and Hosokawa Micron Group announced today that they have entered into an agreement whereby Hosokawa Micron will explore developm... | Press Release - Archive - Page 3
This study explores the use of polydimethylsiloxane (PDMS) reinforced with vapor-grown carbon fibers (VGCF) as a strain sensor. The operational principal of the nanocomposite sensors with VGCF content above the percolation ...
SILRES® BS 5138 is a highly efficient aqueous and water-dilutable emulsion based on a non-reactive Polydimethylsiloxane. It is used especially for hydrophobation of glass- and stone wool. It exhibits high thermal stability and good compatibility with common binders, like phenolic resins. SILRES® BS 5138 can also be used for hydrophobation of porous mineral insulation materials like Perlite, Vermiculite oder Expanded Clay.
POWERSOFT® UP 68 is a nonionic micro emulsion concentrate of a reactive modified aminofunctional polydimethylsiloxane for use in non-yellowing and medium hydrophilic textile finishing.
本聯盟具備完整的黃光微影製程設備及能力,可使用不同底材的光罩(如玻璃、石英、及膠片光罩)及光阻(如正光阻及負光阻)。膠片光罩為快速、低價位的光罩選擇,可符合大部分微流體通道之製作需求。若是微結構小於 5 micrometer,則可選用玻璃或石英光罩。同時本聯盟並具備製作特殊三維微結構,高深寬比微結構以及無光罩曝光特殊光敏材料等之技術。. 軟微影製程(Soft lithography): ...
Fig. 1 Assembly of 3D microstructures using the SEAL process.. (A) Microstructures are fabricated by pressing and heating polymer into a patterned PDMS base mold and delaminating these structures onto a substrate to create the first layer. A second layer is then formed by using a similar molding process against a Teflon surface, which allows the features to remain in the PDMS mold after cooling. The second layer is aligned, placed into contact with the first layer, and sintered by using a mild heating step. (B) Schematic depicting the alignment and sintering equipment, consisting of a mask aligner retrofitted with a Peltier heater. A glass slide containing the first layer is suspended upside down from a fixed mask holder by means of a vacuum while the second layer, still in the PDMS mold, is placed on the wafer chuck, aligned using the stage rotation and translation knobs, put into contact, and heated until they fuse. This approach can be used to create a variety of microstructures, including (C ...
76095 CV/Resume/Work Experience for Design/Software PDMS/PDS, Engineering Piping candidate/job seeker, oil and gas cvs & resumes for hire database search
TY - JOUR. T1 - Micro-patterning of animal cells on PDMS substrates in the presence of serum without use of adhesion inhibitors. AU - De Silva, Mauris N.. AU - Desai, Ravi. AU - Odde, David J.. N1 - Funding Information: The authors would like to thank Yaakov Nahmias, Abhinav Arneja, and Andrew Bicek for their advice in HUVEC and LLCPK1 cell culture and the staff of the Nano Fabrication Center of the University of Minnesota, Dr. Babak Ziaie, and Tingrui Pan for their advice in fabrication technologies. Funding for this project was partially provided by NSF-BITS Grant No. EIA0130875 and through the Microfabricated Neural Networks Interest Group by the Biomedical Engineering Institute at University of Minnesota.. PY - 2004/9. Y1 - 2004/9. KW - Cell patterning. KW - Microcontact printing. KW - Micropatterning. KW - PDMS. UR - http://www.scopus.com/inward/record.url?scp=4544299869&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=4544299869&partnerID=8YFLogxK. U2 - ...
The goal of this study was the preparation, physicochemical characterization, and microbiological evaluation of novel hydroxyapatite doped with silver/polydimethylsiloxane (Ag:HAp-PDMS) composite layers. In the first stage, the deposition of polydimethylsiloxane (PDMS) polymer layer on commercially pure Si disks has been produced in atmospheric pressure corona discharges. Finally, the new silver doped hydroxyapatite/polydimethylsiloxane composite layer has been obtained by the thermal evaporation technique. The Ag:HAp-PDMS composite layers were characterized by various techniques, such as Scanning Electron Microscopy (SEM), Glow Discharge Optical Emission Spectroscopy (GDOES), and X-ray photoelectron spectroscopy (XPS). The antimicrobial activity of the Ag:HAp-PDMS composite layer was assessed against |i|Candida albicans|/i| ATCC 10231 (ATCC—American Type Culture Collection) by culture based and confirmed by SEM and Confocal Laser Scanning Microscopy (CLSM) methods. This is the first study
Aqueous biocompatible tribosystems are desirable for a variety of tissue-contacting medical devices. L-3,4-dihydroxyphenylalanine (DOPA) and lysine (K) peptide mimics of mussel adhesive proteins strongly interact with surfaces and may be useful for surface attachment of lubricating polymers in tribosystems. Here, we describe a significant improvement in lubrication properties of poly (dimethylsiloxane) (PDMS) surfaces when modified with PEG-DOPA-K. Surfaces were characterized by optical and atomic force microscopy, contact angle, PM-IRRAS, and X-ray photoelectron spectroscopy. Such surfaces, tested over the course of 200 rotations (∼8 m in length), maintained an extremely low friction coefficient (μ) (0.03 ± 0.00) compared to bare PDMS (0.98 ± 0.02). These results indicate the potential applications of PEG-DOPA-K for the modification of device surfaces. Extremely low μ values were maintained over relatively long length scales and a range of sliding speeds without the need for substrate ...
Page contains details about poly(dimethylsiloxane)/graphene/graphene oxide/reduced graphene oxide film . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Maintaining a proper pH level is crucial for successful cell culturing. Mammalian cells are commonly cultured in incubators, where the cell culture medium is saturated with a mixture of air and 5% carbon dioxide (CO,inf,2,/inf,). Therefore, to keep cell culture medium pH in an acceptable level outside these incubators, a suitable CO,inf,2,/inf, concentration must be dissolved in the medium. However, it can be very difficult to control and measure precisely local concentration levels. Furthermore, possible undesired concentration gradients generated during long-term cell culturing are almost impossible to detect. Therefore, we have developed a computational model to estimate CO,inf,2,/inf, transport in silicone-based microfluidic devices. An extensive set of experiments was used to validate the finite element model. The model parameters were obtained using suitable measurement set-ups and the model was validated using a fully functional cell cultivation device. The predictions obtained by the ...
The way in which bacterial communities colonize flow in porous media is of importance but basic knowledge on the dynamic of these phenomena is still missing. The aim of this work is to develop microfluidic experiments in order to progress in the understanding of bacteria capture in filters and membranes. PDMS microfluidic devices mimicking filtration processes have been developed to allow a direct dynamic observation of bacteria across 10 or 20 micrometers width microchannels. When filtered in such devices, bacteria behave surprisingly: Escherichia coli, Pseudomonas aeruginosa or Staphylococcus aureus accumulate in the downstream zone of the filter and form large streamers which oscillate in the flow. In this study streamer formation is put in evidence for bacteria suspension in non nutritive conditions in less than one hour. This result is totally different from the one observed in same system with inert particles or dead bacteria which are captured in the bottleneck zone and are accumulated in the
Silicone rubber as used in blood contacting situations usually contains fillers, catalyst and other additives. In this study, polydimethylsiloxane (PDMS) alone was cross-linked by ionizing radiation in a nitrogen atmosphere to produce a pure silicone rubber. This material exhibited a Lee-White clotting time over 45 minutes and a clotting time of 90 minutes when air-blood contact was minimized. Comparable times for commercial silicones are 25 and 60 minutes. In extracorporeal testing using a Mini Lung, an irradiated PDMS coated membrane demonstrated greatly improved thromboresistance over standard silicone rubber membranes. (Author)
Chlorhexidine (CHX) has been incorporated into the composition of polymethyl methacrylate (PMMA) dental restorations to enhance their antimicrobial performance. However, the controlled delivery of CHX remains a challenge. Although previous findings with pure silica or polymer coatings demonstrated the resistance to bacterial adhesion, they did not provide antibacterial activity beyond the coated surface. Polydimethylsiloxane (PDMS) and mesoporous silica nanoparticles (MSNs) are widely used in biomedical science as a transfer medium in drug delivery systems. Here, the MSNs are used to encapsulate CHX, and the combination is added to PDMS. A thin coating film is formed on the PMMA, using oxygen plasma and thermal treatment. The liquid chromatography analysis shows that the coating film has high encapsulation efficiency and loading capacity, with a slow and stable release rate of CHX. The cytotoxicity tests also show that the coating does not affect the proinflammatory cytokines, cellular mitotic activity,
A PED (precision extrusion deposition)/replica molding process enables scaffold guided tissue engineering of a heterocellular microfluidic device. We investigate two types of cell-laden devices: the first with a 3D microfluidic manifold fully embedded in a PDMS (polydimethylsiloxane) substrate and the second a channel network on the surface of the PDMS substrate for cell printing directly into device channels. Fully embedded networks are leak-resistant with simplified construction methods. Channels exposed to the surface are used as mold to hold bioprinted cell-laden matrix for controlled cell placement throughout the network from inlet to outlet. The result is a 3D cell-laden microfluidic device with improved leak-resistance (up to 2.0 mL/min), pervasive diffusion and control of internal architecture.. ...
But what if the product B turned into another product C? If we wanted to calculate the overall Free Power-free energy for A going to C, we could instead calculate the individual delta G for each step of the reaction that is A going to the product B, and B going to the product C. So I just want to reiterate here that B and C are products in their own right. Theyre not transition states. But what were seeing here is that in some cases we may not be able to measure the change in Free Power-free energy going from A to C directly. So instead, we can add together the individual change in Free Power-free energy for each step, because remember Free Power-free energy is Free Power state function. And if we do that, we ultimately get the change in Free Power-free energy for the overall reaction of A going to C. Now one fun way that I kind of remember the state function like quality of delta G, as well as some other variables in chemistry, is that my chemistry professor used to tell us that life is not ...
But what if the product B turned into another product C? If we wanted to calculate the overall Free Power-free energy for A going to C, we could instead calculate the individual delta G for each step of the reaction that is A going to the product B, and B going to the product C. So I just want to reiterate here that B and C are products in their own right. Theyre not transition states. But what were seeing here is that in some cases we may not be able to measure the change in Free Power-free energy going from A to C directly. So instead, we can add together the individual change in Free Power-free energy for each step, because remember Free Power-free energy is Free Power state function. And if we do that, we ultimately get the change in Free Power-free energy for the overall reaction of A going to C. Now one fun way that I kind of remember the state function like quality of delta G, as well as some other variables in chemistry, is that my chemistry professor used to tell us that life is not ...
This work describes the centrifugal casting and fast curing of double-sided, polydimethylsiloxane (PDMS)-based components with microfeatures. Centrifugal casting permits simultaneous patterning of multiple sides of a component and allows control of the thickness of the part in an enclosed mold without entrapment of bubbles. Spinning molds filled with PDMS at thousands of revolutions per minute for several minutes causes entrapped bubbles within the PDMS to migrate toward the axis of rotation or dissolve into solution. To cure the parts quickly (,10 min), active elements heat and cool cavities filled with PDMS after the completion of spinning. Microfluidic channels produced from the process have a low coefficient of variation (,2% for the height and width of channels measured in 20 parts). This process is also capable of molding functional channels in opposite sides of a part as demonstrated through a device with a system of valves typical to multilayer soft lithography.. ...
TY - JOUR. T1 - Axisymmetric polydimethysiloxane microchannels for in vitro hemodynamic studies. AU - Lima, Rui. AU - Oliveira, Monica. AU - Ishikawa, T.. AU - Kaji, H.. AU - Tanaka, S.. AU - Nishizawa, M.. AU - Yamaguchi, T. PY - 2009/9. Y1 - 2009/9. N2 - The current microdevices used for biomedical research are often manufactured using microelectromechanical systems (MEMS) technology. Although it is possible to fabricate precise and reproducible rectangular microchannels using soft lithography techniques, this kind of geometry may not reflect the actual physiology of the microcirculation. Here, we present a simple method to fabricate circular polydimethysiloxane (PDMS) microchannels aiming to mimic an in vivo microvascular environment and suitable for state-of-the-art microscale flow visualization techniques, such as confocal µPIV/PTV. By using a confocal µPTV system individual red blood cells (RBCs) were successfully tracked trough a 75 µm circular PDMS microchannel. The results show that ...
This experiment was adapted by John Kania from one developed by Michael Davis, Microcontact Printing on Gold CD-Rs. PDMS is cured by an organometallic crosslinking reaction to give an optically transparent polymer with the ability to reproduce surface features. In the experiment the polymer is cured in contact with a coin or the printed side…
The first step in microcontact printing is the production of an elastomeric malleable stamp by means of a mold. The stamp is then loaded with a special ink and is pressed onto the metal surface to be printed. The ink sticks to the metal surface and reproduces the microstructure of the stamp in a monomolecular layer. This monolayer acts as a corrosion-resistant mask in the subsequent etching process: the coated areas are not affected, whereas the metal in the uncoated areas is etched away, transferring the microstructure to the metal. Precious and coinage metals are both used, as are materials with oxidic surfaces, such as silicon and aluminum. Each type of surface requires a different type of ink to stick to it: precious and coinage metals need ink molecules that can be bound by means of a metal-sulfur bond. Oxidic surfaces bind molecules with an acid functionality, such as carboxylic acids or phosphonic acids. Substrates that have different types of metals on their surface are thus not easy ...
Nanopoint has developed a novel micro-fabricated live cell containment device called cellTRAYTM designed specifically for imaging live cells. The device enables the precise containment of cells in an optical glass substrate, the size of a standard 1
Nanopoint has developed a novel micro-fabricated live cell containment device called cellTRAYTM designed specifically for imaging live cells. The device enables the precise containment of cells in an optical glass substrate, the size of a standard 1
A living cell has numerous proteins, only a few thousand of which have been identified to date. Cell-free protein synthesis is a useful and promising technique to discover and produce various proteins that might be beneficial for biotechnological, pharmaceutical, and medical applications. For this study, we evaluated the performance and the general applicability of our previously developed microreactor array chip to cell-free protein synthesis by comparisons with a commercially available system. The microreactor array chip comprises a temperature control chip made of glass and a disposable reaction chamber chip made of polydimethylsiloxane (PDMS). For evaluation of the microreactor array chip, rat adipose-type fatty acid binding protein, glyceraldehyde-3-phosphate dehydrogenase, cyclophilin, and firefly luciferase were synthesized from their respective DNA templates using a cell-free extract prepared from ,i,Escherichia coli,/i,. All these proteins were synthesized in the microreactor array ...
A microfluidic device, with a temperature control unit to study the behaviour of temperature sensitive hydrogel, has been designed, simulated and fabricated. The system consists of a PDMS (polydimethylsiloxane) microchannel sealed on a Pyrex substrate with microfabricated titanium electrodes for heating and sensing elements. A thermal insulating layer in-between the electrodes and the substrate was found to increase the heat transfer to the fluid and decrease the lateral heat propagation. The temperature profile and the heat distribution in the system were investigated using the commercial software package CFD-ACE+. The device was electrically and thermally characterised. Such a system, biocompatible and re-usable, could be a potential candidate for biomedical applications such as DNA amplification and protein synthesis ...
The interactions between a cancer cell and its extracellular matrix (ECM) have been the focus of an increasing amount of investigation. The role of the intermediate filament keratin in cancer has also been coming into focus of late, but more research is needed to understand how this piece fits in the puzzle of cytoskeleton-mediated invasion and metastasis. In Panc-1 invasive pancreatic cancer cells, keratin phosphorylation in conjunction with actin inhibition was found to be sufficient to reduce cell area below either treatment alone. We then analyzed intersecting keratin and actin fibers in the cytoskeleton of cyclically stretched cells and found no directional correlation. The role of keratin organization in Panc-1 cellular morphological adaptation and directed migration was then analyzed by culturing cells on cyclically stretched polydimethylsiloxane (PDMS) substrates, nanoscale grates, and rigid pillars. In general, the reorganization of the keratin cytoskeleton allows the cell to become ...
The interactions between a cancer cell and its extracellular matrix (ECM) have been the focus of an increasing amount of investigation. The role of the intermediate filament keratin in cancer has also been coming into focus of late, but more research is needed to understand how this piece fits in the puzzle of cytoskeleton-mediated invasion and metastasis. In Panc-1 invasive pancreatic cancer cells, keratin phosphorylation in conjunction with actin inhibition was found to be sufficient to reduce cell area below either treatment alone. We then analyzed intersecting keratin and actin fibers in the cytoskeleton of cyclically stretched cells and found no directional correlation. The role of keratin organization in Panc-1 cellular morphological adaptation and directed migration was then analyzed by culturing cells on cyclically stretched polydimethylsiloxane (PDMS) substrates, nanoscale grates, and rigid pillars. In general, the reorganization of the keratin cytoskeleton allows the cell to become ...
The mechanical properties of polymeric nanocomposites are strongly affected by the nature of the interphase between filler and matrix, which can be controlled by means of surface chemistry. In this report, we utilize intermodulation atomic force microscopy (ImAFM) to probe local mechanical response with nanometer-scale resolution of poly(dimethylsiloxane) (PDMS),coatings with and without 20 wt of hydrophobic silica nanoparticles. The data evaluation is carried out without inferring any contact mechanics model, and is thus model-independent. ImAFM imaging reveals a small but readily measurable inhomogeneous mechanical response of the pure PDMS surface layer. The analysis of energy dissipation measured with ImAFM showed a lowering of the viscous response due to the presence of the hydrophobic silica nanoparticles in the polymer matrix. An enhanced elastic response was also evident from the in-phase stiffness of the matrix, which was found to increase by a factor of 1.5 in presence of the ...
Abstract: Microfluidic systems integrated with protein and DNA micro- and nanoarrays have been the most sought-after technologies to satisfy the growing demand for high-throughput disease diagnostics. As the sensitivity of these systems relies on the bio-functionalities of the patterned recognition biomolecules, the pr
A microactuator device includes a base with at least one electrode pad and a permeation membrane. Permeation membrane is typically a water-permeable membrane that is able to deform by applying an electric charge to the electrode pad. The actuator device can be incorporated into valve assembly to open and close the valve. The valve assembly can have a reciprocating valve member operated by the deforming of the water-permeable member. Alternatively, the valve assembly can have an opening positioned to cooperate with the water-permeable membrane so that the deformation of the membrane closes the opening.
Recent experimental data dealing with gas-liquid two-phase flow regimes and their transitions in microchannels with circular and near-circular cross-sections are reviewed and compared. It is shown that, for microchannels with hydraulic diameters close to 1 mm, the available data are in good agreement. These data are used as the basis for the development of a simple Weber number-based flow regime map that divides the entire flow map into four zones: a surface tension dominated zone including bubbly and plug flow patterns; an inertia dominated zone representing the annular flow regime; a dispersed/churn flow zone; and a transition zone that consists of other intermittent flow patterns. Comparison is als o made with the limited available data representing channels with slightly larger hydraulic diameters or different cross-sectional geometries, and the effects of channel cross-sectional geometry and size are examined and discussed. The areas in need of further systematic experimental investigation ...
Looking for microfabrication? Find out information about microfabrication. The technology of fabricating microsystems from silicon wafers, using standard semiconductor process technologies in combination with specially developed... Explanation of microfabrication
Solute transport in porous media has always been a focus topic for hydrogeologists. Hydrodynamic dispersion is one of the most important mechanism in solute transport in porous media. Furthermore, dispersion behaviors vary substantially under different saturations. Recent research has introduced fluorescence microscopy methods such as fluorescence recovery after photobleaching (FRAP) ... read more to investigate solute dispersion problem due to its noninvasive nature creating concentration input inside porous medium. In this study, a fluorescence microscopy setup is developed to conduct dispersion experiments in Polydimethylsiloxane (PDMS) microfluidic models. For saturated condition, various dispersion experiments under different flow rates and porosities are conducted. Subsequently, based on the results, a nonlinear negative longitudinal dispersivity-porosity relationship is drawn. Under unsaturated condition, by utilizing the decay nature of the fluorescent molecule, distinction between ...
Conjugated polyelectrolytes (CP) show interesting electrical and optical properties for organic electronics as well as for life science applications. Their possibilities of supramolecular assembly with nanowire like misfolded proteins, amyloids, as well as synthetic polypeptides or DNA forming conducting or luminescent nano composites is highly interesting as being a truly bottom up approach for fabrication of OLEDs, photovoltaics as well as logic devices. The conformation and aggregation dependent luminescence properties from the special class of CPs, Luminescent conjugated polyelectrolytes (LCP), have been utilised and developed as sensors to follow and study biomolecular interactions, DNA hybridisation, protein-protein interactions and staining of living cell cultures and tissue slides. In this thesis we are bringing the evolution a few steps further by applying new types of experimental techniques, such as light scattering and fluorescence correlation spectroscopy, combined with standard ...
TY - JOUR. T1 - Adhesion and migration of cells responding to microtopography. AU - Estévez, Maruxa. AU - Martínez, Elena. AU - Yarwood, Stephen J. AU - Dalby, Matthew J.. AU - Samitier, Josep. N1 - © 2014 Wiley Periodicals, Inc.. PY - 2015/5. Y1 - 2015/5. N2 - It is known that cells respond strongly to microtopography. However, cellular mechanisms of response are unclear. Here, we study wild-type fibroblasts responding to 25 µm(2) posts and compare their response to that of FAK(-/-) fibroblasts and fibroblasts with PMA treatment to stimulate protein kinase C (PKC) and the small g-protein Rac. FAK knockout cells modulated adhesion number and size in a similar way to cells on topography; that is, they used more, smaller adhesions, but migration was almost completely stalled demonstrating the importance of FAK signaling in contact guidance and adhesion turnover. Little similarity, however, was observed to PKC stimulated cells and cells on the topography. Interestingly, with PKC stimulation the ...
Cancer tumor cells exist within a mechanically and heterogeneous microenvironment which undergoes active adjustments throughout neoplastic development chemically. suggest that elevated matrix rigidity and collagen thickness promote improved grip causes, and that metastatic cells generate higher causes AP24534 enzyme inhibitor than non-metastatic cells across all matrix properties examined. Additionally, we discovered that cell dispersing for these cell lines includes a immediate romantic relationship with collagen thickness, but a biphasic romantic relationship with substrate rigidity, indicating that cell region alone will not dictate the magnitude of grip stress AP24534 enzyme inhibitor generation. Jointly, these data claim that mobile contractile drive may play a significant function in metastasis, which the physical properties from the stromal environment may regulate cellular force era. These results are crucial for understanding the physical systems of metastasis as well as the role from ...
Local surface mechanical properties of polymeric nanocomposites play a significant role in theirperformance. Atomic Force Microscopy (AFM) can be used to perform measurements of suchproperties with high lateral resolution. The interphase between filler and matrix, and how it can becontrolled by means of surface chemistry is of particular interest. In this work we compare threeoperating modes of AFM: Tapping mode, PeakForce QNM (Quantitative Nanomechanical Mapping)and Intermodulation AFM (ImAFM), for their ability to capture the tip-surface force and to extractlocal mechanical properties by applying different contact mechanics models. Layers ofpoly(dimethylsiloxane) (PDMS) with and without 20 wt.% of hydrophobic silica nanoparticles werestudied employing these AFM modes. We show that tapping mode AFM can provide qualitativeinformation, but it is insufficient to accurately and quantitatively discriminate surface propertiessince this mode does not allow extraction of the tip-surface force. ...
The overuse and misuse of antibiotics have resulted in the emergence of antibiotic-resistant pathogens along with the perturbation of healthy human microbiota via killing putatively beneficial commensal bacteria. Hence, targeting infectious bacterial pathogens is important for reducing the evolution of antibiotic-resistant bacteria and preserving the endogenous human microbiome. Cell lytic enzymes including bacteriophage endolysins, autolysins, and peptidoglycan targeting enzymes are useful antibiotic alternatives, and genetic information of numerous cell lytic enzymes is currently available. However, the identification of their antimicrobial function and specificity has been limited due to time-intensive protocols to identify their specific targets. Here, we developed Lego-like sandwich microchip for rapidly accessing the function of diverse genes that are suggestive of encoding cell lytic enzymes. This approach can be used to quantify antimicrobial activity in a high-throughput manner by ...
We describe the fabrication of deformable microstructures by low-pressure-soft-microembossing (mu SEmb) that provides in vitro experimental test-beds to investigate the interplay of mechanical and chemical stimuli on cell behavior in a highly controlled environment. Soft microembossing exploits the softness and plasticity of parafilm to fabricate microstructures by pressing a silicon master or an elastomeric poly(dimethylsiloxane) stamp into the parafilm. We demonstrate that a protein-resistant comb polymer can be printed into the raised features of the embossed micro structures, which imparts protein, and hence cell resistance to those regions of the microstructures. These two features of our fabrication methodology-microembossing followed by spatially selective transfer of a nonfouling polymer-forms the core of our strategy to pattern cells within the parafilm microstructures, such that the cells are confined within bottoms of the microstructures. Cell culture experiments demonstrated the ...
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Solid Phase Microextraction with Polystyrene-Poly(dimethylsiloxane) block copolymers, Andrew Schlaus and Rebecca Lyons, Advisor. ...
It is well known in neurophysiology textbooks (see e.g. [12]) that neurons are biological excitable systems possessing an absolute and a relative refractory period. These properties are fundamental for the propagation of nerve impulse and for information processing in the brain. Excitability is a generic property that is also found in biological [8], chemical [11], and optical systems [5]. An excitable system possess a rest state. If perturbed above a certain threshold - the excitable threshold - with a single perturbation, it emits a pulse with a characteristic shape (light pulse in optics, electrical pulse in neurons). If perturbed with two successive pulses above the excitable threshold, it can respond by emitting two identical pulses if the perturbation pulses are well separated temporally. It can emit a unique pulse if the second perturbation occurs too early after the first one : we are then in the absolute refractory period. However, in an intermediate regime, the relative refractory ...
Microchip-based chromatography columns from PharmaFluidics can improve proteomic analyses of biopsies, digests, culture media, and other complex biological samples.
Hence, another aim of this thesis was to evaluate the potential of antibody-targeted polymersomes for the implementation of drug targeting strategies to the brain (section 3.3). For this purpose, the anti-human insulin receptor antibody 83-14 (83 14 mAb) was used as targeting vector because this antibody has been shown to undergo transcytosis in vivo upon binding to the insulin receptor with high affinity (Pardridge et al., 1995). Polymersomes based on poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) [PDMS-b-PMOXA] block copolymers were used in this study. Characterization of polymersomes confirmed their hollow sphere and vesicle-shaped morphology. Fluorescence correlation spectroscopy experiments showed the successful conjugation of the 83 14 mAb to the polymersomes. Flow cytometry analysis revealed binding and uptake of the 83 14 mAb conjugated polymersomes by brain capillary endothelial cells expressing the insulin receptor. Competitive uptake inhibition studies confirmed the ...
The effect of local TTX application on the gamma power. (a) Lay-out of the experimental setup. The rat (1) was kept at 37 °C on a heating blanket (2) and fixed in a stereotaxic frame (3) by ear bars (4) and incisor bar (5). Electrodes were stereotaxically placed in burr holes (6) and local field potentials were by an MPA8 headstage (7) and AM3600 amplifier (8), digitized by a Power 4101 (9), controlled by spike 2 software (10). Guide cannulas (11) were placed such that the inserted quartz infusion cannula (12) perfused TTX locally. 1 μl TTX solution was supplied through a thin tube (13) driven by the MSM micropump (14) driven by an arduino (15) controlled by pulsed voltage output generated by spike software (16). (b) Local field potentials recorded from hippocampus area CA1 before (top trace), 15 min (middle trace) and 30 min (bottom trace) after the start of a 1 μl TTX application over 3 min. (c) Gamma power was determined as a running average from local field potential recordings in the ...
Grünberger, A., Probst, C., Wiechert, W., and Kohlheyer, D. (2012).Towards high-throughput single cell growth optimization and production analysis using picoliter bioreactors. Presented at the Metabolic Engineering IX 2012, Biarritz, France ...
The purpose of this study was to investigate the effects of bovine serum albumin (BSA), lipase and glutaraldehyde on the surface of polydimethylsiloxane (PDMS). PDMS blocks of 15 by 15mm were fabricated using replica ...
In an apparatus for etching a glass substrate according to the present invention, impurities that are attached to the surface of a glass substrate, which are formed by assembling a color filter substrate and a TFT substrate provided in the etching bath filled with etchant, are removed by using ultrasonic oscillation generated from an ultrasonic oscillator, by which a glass substrate having uniform thickness and surface is obtained.
We use a polymer known as PDMS to create molds for use in various experiments. The reason is that PDMS is a very microscope friendly substance, in that it is a clear substance allowing the experimenter to use it as a sort of cover glass. To create a mold one simply makes a template (a negative of the mold you want) and pours a mixture of PDMS with curing agent onto the template. Using a defined volume one can create molds of specified height, width, and depth. We are practicing methods to achieve a substance depth equivalent to that of a glass slide (~1mm). Current templates include a microfluidic channel template and a protein stamper design. The channel template is a square (60mm x 60mm) with 9 channels equally spaced of equal dimensions (~1mm x 60mm x 100um). The PDMS will harden so that the ridges of the template become channels on the mold. When affixed to glass, one will be able to perform single molecule tethering experiments. The other template contains many (about 30 or so) indented ...
Silicone Fluid, Is chemically equivalent to DOW CORNING® 200 FLUID 12,500CS. 100% active, 12,500 cSt, polydimethylsiloxane polymer.